FvH4_7g30370

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000653
Physical Location & Seq
Forward (+)
22135910 .. 22137666
1757 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g30370.t1

Sequence Viewer

Length: 990 bp
ATGGAAGGAGGTTTCGCCCAAAAAACACCGATGAATCAAAGGAAACAGCTCCGAACTAACAAAACAGTAGAAAAAGTTAAGAAAAAGCTCAGAACACCGGTCATCTACAAGGATGTCGAGGAGCTCCTGAGGCTGATGAAGAGGACTCGGCCTGTCCTAAAAGACATAGATCCATCTGCCTGTCGTGTCTGCGCCGGCTCAGTTGATCACATTGAGAACTGCTGTTCCAAGGGAGCATATGCTGTTCGCAGGAGGTGTGTTTGTGGCCATGGTGGTCACTGGCCCTGGGAGAGCAGATGCCCCAAGGACTGGCGCAGACCCTCCAGTATACCCGAGCTCCCGTCCATCCAGGAACTTGAAGAGGAGGTTATGAAGAAAAAGAACAAGGTCAAGGACGAGTGGCACGATGAGGATGATGACTCTGAGGATGAGGATCGTGATGGCTACCCTGGCTACATCCGTTTCGGTGGTGACAGGAAGCAAAAGTTCAAATTTCCAGATTTTGAAGAAGACAAAGAGGAGCTGCTGAGATTGATGAAGAGGATTCGACCTGTTGTCGAAAAAGACATAGATCCATATGCCTGTCTTGTTTGCGCCGGCACAGTTGATCACTTCGATGAGGATGAATGCCCATGCCTAGATAGAATCCCACCAGGCGCAGAACTAGGACCTCAATATGACAAGGTTTGCATTGGTTGTGGTAATATTGGAGAGATCTGCTGTTCCAAGGGAGCATATGCTGTTCGCATGAAGTGTTATTGTCCCCGTGATGATCACTGGCCCTGGGAGCGCAGGTGCCCCAAGGACTGGCGCAGACCCTCCAGTATACCCGAGCTCCCGTCCATCCAGGAACTTGAAGAGGAGGTTACGAAGAAAAAGAACAAGGACATCAAGTACGTGGATGAGGTTATGGTCGAGGATGACAAGAATTCGATCAAGGAGCTCCCGTTCACTGAACAATGCCAGATCTCAACACCAGATGAACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

330

Amino Acids

38.3

Weight (kDa)

5.75

Isoelectric Point (pI)

60.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 783
Acc36I ACCTGC 1 cut(s) 783
AccB1I GGYRCC 1 cut(s) 795
AccB7I CCANNNNNTGG 2 cut(s) 309, 807
AccI GTMKAC 2 cut(s) 328, 826
AclWI GGATC 3 cut(s) 164, 441, 566
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 2 cut(s) 491, 928
AfaI GTAC 1 cut(s) 896
AfiI CCNNNNNNNGG 2 cut(s) 309, 807
AgeI ACCGGT 1 cut(s) 97
AgsI TTSAA 4 cut(s) 359, 490, 506, 857
AhdI GACNNNNNGTC 1 cut(s) 554
AjnI CCWGG 6 cut(s) 284, 348, 448, 652, 782, 846
AloI GAACNNNNNNTCC 4 cut(s) 209, 241, 932, 964
AluBI AGCT 7 cut(s) 49, 88, 124, 337, 523, 835, 943
AluI AGCT 7 cut(s) 49, 88, 124, 337, 523, 835, 943
Alw21I GWGCWC 4 cut(s) 126, 339, 837, 945
AlwI GGATC 3 cut(s) 164, 441, 566
Ama87I CYCGRG 2 cut(s) 332, 830
AoxI GGCC 4 cut(s) 149, 265, 281, 779
ApeKI GCWGC 1 cut(s) 523
ApoI RAATTY 2 cut(s) 491, 928
AsiGI ACCGGT 1 cut(s) 97
AspLEI GCGC 6 cut(s) 194, 315, 596, 659, 792, 813
AspS9I GGNCC 3 cut(s) 282, 668, 780
AsuHPI GGTGA 1 cut(s) 482
AvaI CYCGRG 2 cut(s) 332, 830
AvaII GGWCC 1 cut(s) 668
AxyI CCTNAGG 1 cut(s) 128
BaeGI GKGCMC 1 cut(s) 800
BalI TGGCCA 1 cut(s) 267
BanI GGYRCC 1 cut(s) 795
BanII GRGCYC 4 cut(s) 126, 339, 837, 945
BbsI GAAGAC 1 cut(s) 516
Bbv12I GWGCWC 4 cut(s) 126, 339, 837, 945
BbvI GCAGC 1 cut(s) 510
BccI CCATC 4 cut(s) 181, 353, 434, 851
BciT130I CCWGG 6 cut(s) 286, 350, 450, 654, 784, 848
BclI TGATCA 3 cut(s) 205, 607, 772
BfaI CTAG 2 cut(s) 638, 665
BfuAI ACCTGC 1 cut(s) 783
BglII AGATCT 2 cut(s) 714, 966
BisI GCNGC 1 cut(s) 524
BlsI GCNGC 1 cut(s) 525
Bme1390I CCNGG 6 cut(s) 286, 350, 450, 654, 784, 848
Bme18I GGWCC 1 cut(s) 668
BmeRI GACNNNNNGTC 1 cut(s) 554
BmeT110I CYCGRG 2 cut(s) 332, 830
BmgT120I GGNCC 3 cut(s) 282, 668, 780
BmiI GGNNCC 1 cut(s) 797
BmrFI CCNGG 6 cut(s) 286, 350, 450, 654, 784, 848
BmsI GCATC 1 cut(s) 287
BpiI GAAGAC 1 cut(s) 516
BpmI CTGGAG 2 cut(s) 307, 805
BsaAI YACGTR 1 cut(s) 898
BsaBI GATNNNNATC 1 cut(s) 432
BsaWI WCCGGW 1 cut(s) 97
BsaXI ACNNNNNCTCC 8 cut(s) 321, 351, 779, 809, 819, 849, 932, 962
Bsc4I CCNNNNNNNGG 2 cut(s) 309, 807
Bse118I RCCGGY 3 cut(s) 97, 194, 596
Bse1I ACTGG 6 cut(s) 284, 314, 324, 782, 812, 822
Bse21I CCTNAGG 1 cut(s) 128
Bse8I GATNNNNATC 1 cut(s) 432
BseBI CCWGG 6 cut(s) 286, 350, 450, 654, 784, 848
BseGI GGATG 9 cut(s) 118, 345, 418, 433, 456, 628, 843, 907, 925
BseJI GATNNNNATC 1 cut(s) 432
BseLI CCNNNNNNNGG 2 cut(s) 309, 807
BseMII CTCAG 5 cut(s) 103, 119, 213, 414, 518
BseNI ACTGG 6 cut(s) 284, 314, 324, 782, 812, 822
BseRI GAGGAG 4 cut(s) 134, 377, 533, 875
BseSI GKGCMC 1 cut(s) 800
BseXI GCAGC 1 cut(s) 510
BshFI GGCC 4 cut(s) 151, 267, 283, 781
BshNI GGYRCC 1 cut(s) 795
BshTI ACCGGT 1 cut(s) 97
BsiHKAI GWGCWC 4 cut(s) 126, 339, 837, 945
BsiHKCI CYCGRG 2 cut(s) 332, 830
BsiSI CCGG 3 cut(s) 98, 195, 597
BslFI GGGAC 1 cut(s) 747
BslI CCNNNNNNNGG 2 cut(s) 309, 807
BsmFI GGGAC 1 cut(s) 747
BsmI GAATGC 1 cut(s) 632
BsnI GGCC 4 cut(s) 151, 267, 283, 781
BsoBI CYCGRG 2 cut(s) 332, 830
Bsp1286I GDGCHC 5 cut(s) 126, 339, 800, 837, 945
Bsp143I GATC 9 cut(s) 169, 205, 433, 571, 607, 714, 772, 933, 966
Bsp19I CCATGG 1 cut(s) 268
BspANI GGCC 4 cut(s) 151, 267, 283, 781
BspCNI CTCAG 5 cut(s) 102, 120, 212, 415, 519
BspLI GGNNCC 1 cut(s) 797
BspMI ACCTGC 1 cut(s) 783
BspPI GGATC 3 cut(s) 164, 441, 566
BspT107I GGYRCC 1 cut(s) 795
BsrFI RCCGGY 3 cut(s) 97, 194, 596
BsrI ACTGG 6 cut(s) 284, 314, 324, 782, 812, 822
BssAI RCCGGY 3 cut(s) 97, 194, 596
BssMI GATC 9 cut(s) 169, 205, 433, 571, 607, 714, 772, 933, 966
BssNAI GTATAC 2 cut(s) 329, 827
BssT1I CCWWGG 5 cut(s) 228, 268, 303, 726, 801
Bst1107I GTATAC 2 cut(s) 329, 827
Bst2UI CCWGG 6 cut(s) 286, 350, 450, 654, 784, 848
Bst4CI ACNGT 2 cut(s) 67, 604
Bst6I CTCTTC 4 cut(s) 134, 354, 533, 852
BstBAI YACGTR 1 cut(s) 898
BstC8I GCNNGC 2 cut(s) 196, 598
BstDEI CTNAG 5 cut(s) 89, 128, 199, 423, 527
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 9 cut(s) 118, 345, 418, 433, 456, 628, 843, 907, 925
BstHHI GCGC 6 cut(s) 194, 315, 596, 659, 792, 813
BstKTI GATC 9 cut(s) 172, 208, 436, 574, 610, 717, 775, 936, 969
BstMBI GATC 9 cut(s) 169, 205, 433, 571, 607, 714, 772, 933, 966
BstMWI GCNNNNNNNGC 3 cut(s) 130, 450, 787
BstNI CCWGG 6 cut(s) 286, 350, 450, 654, 784, 848
BstSCI CCNGG 6 cut(s) 284, 348, 448, 652, 782, 846
BstSLI GKGCMC 1 cut(s) 800
BstV1I GCAGC 1 cut(s) 510
BstV2I GAAGAC 1 cut(s) 516
BstX2I RGATCY 4 cut(s) 169, 571, 714, 966
BstYI RGATCY 4 cut(s) 169, 571, 714, 966
BstZ17I GTATAC 2 cut(s) 329, 827
Bsu36I CCTNAGG 1 cut(s) 128
BsuRI GGCC 4 cut(s) 151, 267, 283, 781
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 9 cut(s) 118, 345, 418, 433, 456, 628, 843, 907, 925
BtsIMutI CAGTG 3 cut(s) 277, 775, 951
BveI ACCTGC 1 cut(s) 783
Cac8I GCNNGC 2 cut(s) 196, 598
CfoI GCGC 6 cut(s) 194, 315, 596, 659, 792, 813
Cfr10I RCCGGY 3 cut(s) 97, 194, 596
Cfr13I GGNCC 3 cut(s) 282, 668, 780
Csp6I GTAC 1 cut(s) 895
CspAI ACCGGT 1 cut(s) 97
CviAII CATG 3 cut(s) 269, 633, 748
CviQI GTAC 1 cut(s) 895
DdeI CTNAG 5 cut(s) 89, 128, 199, 423, 527
DpnI GATC 9 cut(s) 171, 207, 435, 573, 609, 716, 774, 935, 968
DpnII GATC 9 cut(s) 169, 205, 433, 571, 607, 714, 772, 933, 966
DriI GACNNNNNGTC 1 cut(s) 554
EaeI YGGCCR 1 cut(s) 265
Eam1104I CTCTTC 4 cut(s) 134, 354, 533, 852
Eam1105I GACNNNNNGTC 1 cut(s) 554
EarI CTCTTC 4 cut(s) 134, 354, 533, 852
Ecl136II GAGCTC 4 cut(s) 124, 337, 835, 943
Eco130I CCWWGG 5 cut(s) 228, 268, 303, 726, 801
Eco24I GRGCYC 4 cut(s) 126, 339, 837, 945
Eco47I GGWCC 1 cut(s) 668
Eco53kI GAGCTC 4 cut(s) 124, 337, 835, 943
Eco81I CCTNAGG 1 cut(s) 128
Eco88I CYCGRG 2 cut(s) 332, 830
EcoICRI GAGCTC 4 cut(s) 124, 337, 835, 943
EcoO109I RGGNCCY 1 cut(s) 668
EcoRI GAATTC 1 cut(s) 928
EcoRII CCWGG 6 cut(s) 284, 348, 448, 652, 782, 846
EcoT14I CCWWGG 5 cut(s) 228, 268, 303, 726, 801
EcoT38I GRGCYC 4 cut(s) 126, 339, 837, 945
ErhI CCWWGG 5 cut(s) 228, 268, 303, 726, 801
FaeI CATG 3 cut(s) 272, 636, 751
FaqI GGGAC 1 cut(s) 747
FatI CATG 3 cut(s) 268, 632, 747
FauNDI CATATG 3 cut(s) 238, 577, 736
FbaI TGATCA 3 cut(s) 205, 607, 772
FblI GTMKAC 2 cut(s) 328, 826
Fnu4HI GCNGC 1 cut(s) 524
FokI GGATG 9 cut(s) 125, 332, 425, 440, 443, 635, 830, 914, 932
FriOI GRGCYC 4 cut(s) 126, 339, 837, 945
Fsp4HI GCNGC 1 cut(s) 524
FspBI CTAG 2 cut(s) 638, 665
GlaI GCGC 6 cut(s) 193, 314, 595, 658, 791, 812
GluI GCNGC 1 cut(s) 524
GsuI CTGGAG 2 cut(s) 307, 805
HaeIII GGCC 4 cut(s) 151, 267, 283, 781
HapII CCGG 3 cut(s) 98, 195, 597
HhaI GCGC 6 cut(s) 194, 315, 596, 659, 792, 813
Hin1II CATG 3 cut(s) 272, 636, 751
Hin6I GCGC 6 cut(s) 192, 313, 594, 657, 790, 811
HinP1I GCGC 6 cut(s) 192, 313, 594, 657, 790, 811
HinfI GANTC 5 cut(s) 34, 145, 419, 544, 645
HpaII CCGG 3 cut(s) 98, 195, 597
HphI GGTGA 1 cut(s) 482
Hpy166II GTNNAC 3 cut(s) 329, 827, 951
Hpy188I TCNGA 3 cut(s) 53, 92, 424
Hpy188III TCNNGA 3 cut(s) 127, 437, 497
Hpy8I GTNNAC 3 cut(s) 329, 827, 951
HpyCH4III ACNGT 2 cut(s) 67, 604
HpyCH4IV ACGT 1 cut(s) 897
HpyCH4V TGCA 1 cut(s) 690
HpyF10VI GCNNNNNNNGC 3 cut(s) 130, 450, 787
HpyF3I CTNAG 5 cut(s) 89, 128, 199, 423, 527
HpySE526I ACGT 1 cut(s) 897
Hsp92II CATG 3 cut(s) 272, 636, 751
HspAI GCGC 6 cut(s) 192, 313, 594, 657, 790, 811
KroI GCCGGC 2 cut(s) 194, 596
KroNI GCCGGC 2 cut(s) 196, 598
Ksp22I TGATCA 3 cut(s) 205, 607, 772
Kzo9I GATC 9 cut(s) 169, 205, 433, 571, 607, 714, 772, 933, 966
Lsp1109I GCAGC 1 cut(s) 510
LweI GCATC 1 cut(s) 287
MaeI CTAG 2 cut(s) 638, 665
MaeII ACGT 1 cut(s) 897
MaeIII GTNAC 3 cut(s) 275, 470, 865
MalI GATC 9 cut(s) 171, 207, 435, 573, 609, 716, 774, 935, 968
MboI GATC 9 cut(s) 169, 205, 433, 571, 607, 714, 772, 933, 966
MboII GAAGA 8 cut(s) 151, 371, 385, 518, 521, 550, 869, 883
MflI RGATCY 4 cut(s) 169, 571, 714, 966
MhlI GDGCHC 5 cut(s) 126, 339, 800, 837, 945
MlsI TGGCCA 1 cut(s) 267
MluCI AATT 2 cut(s) 491, 928
MluNI TGGCCA 1 cut(s) 267
MlyI GAGTC 2 cut(s) 139, 413
Mox20I TGGCCA 1 cut(s) 267
MroNI GCCGGC 2 cut(s) 194, 596
MscI TGGCCA 1 cut(s) 267
MseI TTAA 1 cut(s) 78
MslI CAYNNNNRTG 1 cut(s) 615
Msp20I TGGCCA 1 cut(s) 267
MspI CCGG 3 cut(s) 98, 195, 597
MspR9I CCNGG 6 cut(s) 286, 350, 450, 654, 784, 848
Mva1269I GAATGC 1 cut(s) 632
MvaI CCWGG 6 cut(s) 286, 350, 450, 654, 784, 848
MwoI GCNNNNNNNGC 3 cut(s) 130, 450, 787
NaeI GCCGGC 2 cut(s) 196, 598
NcoI CCATGG 1 cut(s) 268
NdeI CATATG 3 cut(s) 238, 577, 736
NdeII GATC 9 cut(s) 169, 205, 433, 571, 607, 714, 772, 933, 966
NgoMIV GCCGGC 2 cut(s) 194, 596
NlaIII CATG 3 cut(s) 272, 636, 751
NlaIV GGNNCC 1 cut(s) 797
NmeAIII GCCGAG 1 cut(s) 127
NmuCI GTSAC 2 cut(s) 275, 470
PaqCI CACCTGC 1 cut(s) 783
PasI CCCWGGG 2 cut(s) 285, 783
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PctI GAATGC 1 cut(s) 632
PdiI GCCGGC 2 cut(s) 196, 598
PfeI GAWTC 3 cut(s) 34, 544, 645
PflMI CCANNNNNTGG 2 cut(s) 309, 807
PfoI TCCNGGA 2 cut(s) 348, 846
PinAI ACCGGT 1 cut(s) 97
PkrI GCNGC 1 cut(s) 525
PleI GAGTC 2 cut(s) 139, 413
PpsI GAGTC 2 cut(s) 139, 413
Ppu21I YACGTR 1 cut(s) 898
PpuMI RGGWCCY 1 cut(s) 668
Psp124BI GAGCTC 4 cut(s) 126, 339, 837, 945
Psp5II RGGWCCY 1 cut(s) 668
Psp6I CCWGG 6 cut(s) 284, 348, 448, 652, 782, 846
PspGI CCWGG 6 cut(s) 284, 348, 448, 652, 782, 846
PspN4I GGNNCC 1 cut(s) 797
PspPI GGNCC 3 cut(s) 282, 668, 780
PspPPI RGGWCCY 1 cut(s) 668
PsuI RGATCY 4 cut(s) 169, 571, 714, 966
RsaI GTAC 1 cut(s) 896
RsaNI GTAC 1 cut(s) 895
RseI CAYNNNNRTG 1 cut(s) 615
SacI GAGCTC 4 cut(s) 126, 339, 837, 945
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 1 cut(s) 524
Sau3AI GATC 9 cut(s) 169, 205, 433, 571, 607, 714, 772, 933, 966
Sau96I GGNCC 3 cut(s) 282, 668, 780
SchI GAGTC 2 cut(s) 139, 413
ScrFI CCNGG 6 cut(s) 286, 350, 450, 654, 784, 848
SduI GDGCHC 5 cut(s) 126, 339, 800, 837, 945
SfaNI GCATC 1 cut(s) 287
SinI GGWCC 1 cut(s) 668
SmiMI CAYNNNNRTG 1 cut(s) 615
Sse9I AATT 2 cut(s) 491, 928
SspI AATATT 1 cut(s) 706
SspMI CTAG 2 cut(s) 638, 665
SstI GAGCTC 4 cut(s) 126, 339, 837, 945
StyD4I CCNGG 6 cut(s) 284, 348, 448, 652, 782, 846
StyI CCWWGG 5 cut(s) 228, 268, 303, 726, 801
TaaI ACNGT 2 cut(s) 67, 604
TaiI ACGT 1 cut(s) 900
TaqI TCGA 6 cut(s) 117, 547, 558, 615, 915, 932
TasI AATT 2 cut(s) 491, 928
TfiI GAWTC 3 cut(s) 34, 544, 645
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TscAI CASTG 3 cut(s) 284, 782, 958
TseFI GTSAC 2 cut(s) 275, 470
TseI GCWGC 1 cut(s) 523
Tsp45I GTSAC 2 cut(s) 275, 470
TspDTI ATGAA 6 cut(s) 47, 152, 386, 551, 639, 764
TspGWI ACGGA 1 cut(s) 449
TspRI CASTG 3 cut(s) 284, 782, 958
Van91I CCANNNNNTGG 2 cut(s) 309, 807
VpaK11BI GGWCC 1 cut(s) 668
XapI RAATTY 2 cut(s) 491, 928
XmiI GTMKAC 2 cut(s) 328, 826
XspI CTAG 2 cut(s) 638, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.