Rh2BG484700

Eukaryotic family of unknown function (DUF1754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002762
Physical Location & Seq
Reverse (-)
68489593 .. 68492052
2460 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG484700.1

Sequence Viewer

Length: 465 bp
ATGGGATCGCCAGCGTCGTTTTCACAGGGAAGACAAGCGATAGATTTCGGGAAAATGTCGTCATATGAGAATGTCGTTGGTGGGAAGCTCAAGCTTAAGGGAAAGGCTCTGGATGTGAAGGCTGCTGGGATTAAGAAGAAAAAGAAACATAAGAAACATCAAGATCAATTATCTCTTGTCACAGAAAAGGAGCTCGCAGCTGGGGGAAGTACAGAAGAGTTGACCAATCCTGATGAGGAAGATATGAATGATGACAACAAATCAAGAGAGGATGGGAAGGCTGCTAGTTATGACGAATATTTGACGCCTGCAGAGAGACGATATATAGAACAGAGAGAGCAAATTGATAATCACAGGTTGGCCAAGACTGCAAATAAATCACACCGTGACAGAATTCAAGACTTCAACCAGTATCTGGCCAACATGAGTGAGCATTATGACATTCCTAAAGTTGGTCCAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 map00100 map01100 map01110
Pfam Domains
Protein Families
DUF1754 ERG4_ERG24

Protein Analysis

154

Amino Acids

17.31

Weight (kDa)

7.88

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM32A PF08555 22 - 117 3.8e-15 FAM32A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 415
AclWI GGATC 1 cut(s) 13
AcoI YGGCCR 2 cut(s) 360, 417
AcsI RAATTY 1 cut(s) 393
AcyI GRCGYC 1 cut(s) 305
AdeI CACNNNGTG 1 cut(s) 386
AfaI GTAC 1 cut(s) 211
AfiI CCNNNNNNNGG 2 cut(s) 415, 452
AflII CTTAAG 1 cut(s) 95
AgsI TTSAA 2 cut(s) 398, 406
AjnI CCWGG 1 cut(s) 457
AluBI AGCT 4 cut(s) 88, 94, 193, 200
AluI AGCT 4 cut(s) 88, 94, 193, 200
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 1 cut(s) 310
AlwI GGATC 1 cut(s) 13
AlwNI CAGNNNCTG 1 cut(s) 415
AoxI GGCC 2 cut(s) 360, 417
ApeKI GCWGC 3 cut(s) 122, 197, 281
ApoI RAATTY 1 cut(s) 393
AspS9I GGNCC 1 cut(s) 455
AvaII GGWCC 1 cut(s) 455
BalI TGGCCA 2 cut(s) 362, 419
BanII GRGCYC 1 cut(s) 195
BbsI GAAGAC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 3 cut(s) 109, 209, 268
BccI CCATC 1 cut(s) 266
BciT130I CCWGG 1 cut(s) 459
BcoDI GTCTC 1 cut(s) 310
BfaI CTAG 1 cut(s) 285
BfmI CTRYAG 1 cut(s) 309
BfrI CTTAAG 1 cut(s) 95
BisI GCNGC 3 cut(s) 123, 198, 282
BlsI GCNGC 3 cut(s) 124, 199, 283
Bme1390I CCNGG 1 cut(s) 459
Bme18I GGWCC 1 cut(s) 455
BmgT120I GGNCC 1 cut(s) 455
BmrFI CCNGG 1 cut(s) 459
BpiI GAAGAC 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 74
BsaHI GRCGYC 1 cut(s) 305
Bsc4I CCNNNNNNNGG 2 cut(s) 415, 452
Bse1I ACTGG 1 cut(s) 409
BseBI CCWGG 1 cut(s) 459
BseGI GGATG 2 cut(s) 118, 277
BseLI CCNNNNNNNGG 2 cut(s) 415, 452
BseNI ACTGG 1 cut(s) 409
BseXI GCAGC 3 cut(s) 109, 209, 268
BseYI CCCAGC 2 cut(s) 125, 200
BshFI GGCC 2 cut(s) 362, 419
BsiHKAI GWGCWC 1 cut(s) 195
BslI CCNNNNNNNGG 2 cut(s) 415, 452
BsmAI GTCTC 1 cut(s) 310
BsmBI CGTCTC 1 cut(s) 310
BsnI GGCC 2 cut(s) 362, 419
Bsp1286I GDGCHC 1 cut(s) 195
Bsp143I GATC 2 cut(s) 5, 163
BspANI GGCC 2 cut(s) 362, 419
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 1 cut(s) 13
BspTI CTTAAG 1 cut(s) 95
BsrI ACTGG 1 cut(s) 409
BssMI GATC 2 cut(s) 5, 163
BssNI GRCGYC 1 cut(s) 305
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 1 cut(s) 386
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 1 cut(s) 305
BstAFI CTTAAG 1 cut(s) 95
BstC8I GCNNGC 3 cut(s) 12, 195, 309
BstF5I GGATG 2 cut(s) 118, 277
BstKTI GATC 2 cut(s) 8, 166
BstMAI GTCTC 1 cut(s) 310
BstMBI GATC 2 cut(s) 5, 163
BstMWI GCNNNNNNNGC 1 cut(s) 368
BstNI CCWGG 1 cut(s) 459
BstSCI CCNGG 1 cut(s) 457
BstSFI CTRYAG 1 cut(s) 309
BstV1I GCAGC 3 cut(s) 109, 209, 268
BstV2I GAAGAC 1 cut(s) 37
BsuRI GGCC 2 cut(s) 362, 419
BtsCI GGATG 2 cut(s) 118, 277
Cac8I GCNNGC 3 cut(s) 12, 195, 309
CaiI CAGNNNCTG 1 cut(s) 415
Cfr13I GGNCC 1 cut(s) 455
CseI GACGC 2 cut(s) 3, 313
Csp6I GTAC 1 cut(s) 210
CviAII CATG 1 cut(s) 424
CviQI GTAC 1 cut(s) 210
DpnI GATC 2 cut(s) 7, 165
DpnII GATC 2 cut(s) 5, 163
DraIII CACNNNGTG 1 cut(s) 386
EaeI YGGCCR 2 cut(s) 360, 417
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 193
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 455
Eco53kI GAGCTC 1 cut(s) 193
EcoICRI GAGCTC 1 cut(s) 193
EcoRI GAATTC 1 cut(s) 393
EcoRII CCWGG 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 195
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 1 cut(s) 427
FaiI YATR 9 cut(s) 64, 66, 150, 245, 291, 324, 326, 425, 438
FatI CATG 1 cut(s) 423
FauNDI CATATG 1 cut(s) 64
Fnu4HI GCNGC 3 cut(s) 123, 198, 282
FokI GGATG 2 cut(s) 125, 284
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 3 cut(s) 123, 198, 282
FspBI CTAG 1 cut(s) 285
GluI GCNGC 3 cut(s) 123, 198, 282
GsaI CCCAGC 2 cut(s) 129, 204
HaeIII GGCC 2 cut(s) 362, 419
HgaI GACGC 2 cut(s) 3, 313
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 1 cut(s) 427
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HindIII AAGCTT 1 cut(s) 92
Hpy166II GTNNAC 1 cut(s) 222
Hpy188III TCNNGA 6 cut(s) 49, 110, 161, 230, 264, 398
Hpy8I GTNNAC 1 cut(s) 222
Hpy99I CGWCG 1 cut(s) 19
HpyAV CCTTC 2 cut(s) 112, 271
HpyCH4III ACNGT 1 cut(s) 386
HpyCH4V TGCA 2 cut(s) 311, 371
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 1 cut(s) 427
Kzo9I GATC 2 cut(s) 5, 163
LmnI GCTCC 1 cut(s) 190
Lsp1109I GCAGC 3 cut(s) 109, 209, 268
MaeI CTAG 1 cut(s) 285
MaeIII GTNAC 2 cut(s) 178, 386
MalI GATC 2 cut(s) 7, 165
MboI GATC 2 cut(s) 5, 163
MboII GAAGA 4 cut(s) 42, 148, 227, 251
MhlI GDGCHC 1 cut(s) 195
MlsI TGGCCA 2 cut(s) 362, 419
MluCI AATT 3 cut(s) 167, 342, 393
MluNI TGGCCA 2 cut(s) 362, 419
MnlI CCTC 2 cut(s) 229, 262
Mox20I TGGCCA 2 cut(s) 362, 419
MscI TGGCCA 2 cut(s) 362, 419
MseI TTAA 2 cut(s) 96, 132
Msp20I TGGCCA 2 cut(s) 362, 419
MspA1I CMGCKG 1 cut(s) 200
MspCI CTTAAG 1 cut(s) 95
MspR9I CCNGG 1 cut(s) 459
MvaI CCWGG 1 cut(s) 459
MwoI GCNNNNNNNGC 1 cut(s) 368
NdeI CATATG 1 cut(s) 64
NdeII GATC 2 cut(s) 5, 163
NlaIII CATG 1 cut(s) 427
NmuCI GTSAC 2 cut(s) 178, 386
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PflMI CCANNNNNTGG 1 cut(s) 415
PkrI GCNGC 3 cut(s) 124, 199, 283
Psp124BI GAGCTC 1 cut(s) 195
Psp6I CCWGG 1 cut(s) 457
PspFI CCCAGC 2 cut(s) 125, 200
PspGI CCWGG 1 cut(s) 457
PspPI GGNCC 1 cut(s) 455
PstI CTGCAG 1 cut(s) 313
PstNI CAGNNNCTG 1 cut(s) 415
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
SacI GAGCTC 1 cut(s) 195
SaqAI TTAA 2 cut(s) 96, 132
SatI GCNGC 3 cut(s) 123, 198, 282
Sau3AI GATC 2 cut(s) 5, 163
Sau96I GGNCC 1 cut(s) 455
ScrFI CCNGG 1 cut(s) 459
SduI GDGCHC 1 cut(s) 195
SetI ASST 5 cut(s) 90, 96, 195, 202, 359
SfcI CTRYAG 1 cut(s) 309
SinI GGWCC 1 cut(s) 455
SmlI CTYRAG 2 cut(s) 89, 95
SmoI CTYRAG 2 cut(s) 89, 95
Sse9I AATT 3 cut(s) 167, 342, 393
SspI AATATT 1 cut(s) 299
SspMI CTAG 1 cut(s) 285
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 1 cut(s) 457
TaaI ACNGT 1 cut(s) 386
TasI AATT 3 cut(s) 167, 342, 393
TatI WGTACW 1 cut(s) 209
Tru1I TTAA 2 cut(s) 96, 132
Tru9I TTAA 2 cut(s) 96, 132
TseFI GTSAC 2 cut(s) 178, 386
TseI GCWGC 3 cut(s) 122, 197, 281
Tsp45I GTSAC 2 cut(s) 178, 386
TspDTI ATGAA 1 cut(s) 260
Van91I CCANNNNNTGG 1 cut(s) 415
Vha464I CTTAAG 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 455
XapI RAATTY 1 cut(s) 393
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.