MD15G1110000.v1.1

FAM32A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002762
Physical Location & Seq
Reverse (-)
7697376 .. 7698237
862 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1110000.v1.1.491

Sequence Viewer

Length: 387 bp
ATGTCTGCGTTCGATAACGTGGTTGTAGGGAAGCTGAAGCTGAAAGGCAAAGCCTTGGACGTCAAAGGCGGTGCGATTTCGAAGAAAAAGAAGAAGAAGAAACAGAGCGCATTTTATCACCAATTGTCTGAGGCCTCCACAGGTGGAGAAGCAGTGACAGATGATGCTGAGAACGAAGGCGGCATTGGCACGATGCAGGCTGCTGCTTCTGCTTATGGTCATCTGACACCGGCGGAGAGGCGGTACCTCGAGCAGACAGAGAGGATTCAACGCCAGAGGCTTTCCAAGATTGCTCGAAAATCGCACCGCGATCGGGTTCAGGAATTCAATCAGTATCTGGCTAATCTGAGTGAGCATTATGACATTCCTAAAGTAGGCCCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1754

Protein Analysis

129

Amino Acids

14.13

Weight (kDa)

9.73

Isoelectric Point (pI)

29.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM32A PF08555 5 - 91 3.4e-12 FAM32A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 63
Acc65I GGTACC 1 cut(s) 243
AccB1I GGYRCC 1 cut(s) 243
AccII CGCG 1 cut(s) 309
AciI CCGC 5 cut(s) 69, 180, 233, 241, 307
AcsI RAATTY 1 cut(s) 323
AcuI CTGAAG 1 cut(s) 56
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 1 cut(s) 245
AfiI CCNNNNNNNGG 2 cut(s) 313, 374
AgsI TTSAA 2 cut(s) 269, 328
AjnI CCWGG 1 cut(s) 379
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AluBI AGCT 2 cut(s) 34, 40
AluI AGCT 2 cut(s) 34, 40
AlwNI CAGNNNCTG 1 cut(s) 337
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 2 cut(s) 132, 376
ApeKI GCWGC 2 cut(s) 200, 203
ApoI RAATTY 1 cut(s) 323
Asp718I GGTACC 1 cut(s) 243
AspLEI GCGC 1 cut(s) 110
AspS9I GGNCC 1 cut(s) 377
AsuHPI GGTGA 1 cut(s) 110
AsuII TTCGAA 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 248
BanI GGYRCC 1 cut(s) 243
BbvI GCAGC 2 cut(s) 187, 190
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciT130I CCWGG 1 cut(s) 381
BisI GCNGC 3 cut(s) 181, 201, 204
BlsI GCNGC 3 cut(s) 182, 202, 205
Bme1390I CCNGG 1 cut(s) 381
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 377
BmiI GGNNCC 1 cut(s) 245
BmrFI CCNGG 1 cut(s) 381
BmsI GCATC 2 cut(s) 154, 183
Bpu14I TTCGAA 1 cut(s) 80
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 2 cut(s) 54, 379
BsaXI ACNNNNNCTCC 4 cut(s) 138, 168, 227, 257
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 374
Bse118I RCCGGY 1 cut(s) 229
BseBI CCWGG 1 cut(s) 381
BseDI CCNNGG 2 cut(s) 54, 379
BseLI CCNNNNNNNGG 2 cut(s) 313, 374
BseMII CTCAG 3 cut(s) 120, 159, 338
BseXI GCAGC 2 cut(s) 187, 190
Bsh1236I CGCG 1 cut(s) 309
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 2 cut(s) 134, 378
BshNI GGYRCC 1 cut(s) 243
BsiEI CGRYCG 1 cut(s) 313
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 1 cut(s) 230
BslI CCNNNNNNNGG 2 cut(s) 313, 374
BsnI GGCC 2 cut(s) 134, 378
BsoBI CYCGRG 1 cut(s) 248
Bsp119I TTCGAA 1 cut(s) 80
Bsp143I GATC 1 cut(s) 310
BspACI CCGC 5 cut(s) 69, 180, 233, 241, 307
BspANI GGCC 2 cut(s) 134, 378
BspCNI CTCAG 3 cut(s) 121, 160, 339
BspFNI CGCG 1 cut(s) 309
BspLI GGNNCC 1 cut(s) 245
BspT104I TTCGAA 1 cut(s) 80
BspT107I GGYRCC 1 cut(s) 243
BsrFI RCCGGY 1 cut(s) 229
BssAI RCCGGY 1 cut(s) 229
BssECI CCNNGG 2 cut(s) 54, 379
BssMI GATC 1 cut(s) 310
BssNI GRCGYC 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 381
BstACI GRCGYC 1 cut(s) 60
BstBI TTCGAA 1 cut(s) 80
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 3 cut(s) 129, 168, 347
BstENI CCTNNNNNAGG 1 cut(s) 372
BstFNI CGCG 1 cut(s) 309
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 1 cut(s) 313
BstMBI GATC 1 cut(s) 310
BstMCI CGRYCG 1 cut(s) 313
BstMWI GCNNNNNNNGC 2 cut(s) 186, 209
BstNI CCWGG 1 cut(s) 381
BstSCI CCNGG 1 cut(s) 379
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 2 cut(s) 187, 190
BsuRI GGCC 2 cut(s) 134, 378
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 1 cut(s) 159
Cac8I GCNNGC 1 cut(s) 198
CaiI CAGNNNCTG 1 cut(s) 337
CfoI GCGC 1 cut(s) 110
Cfr10I RCCGGY 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 377
Csp6I GTAC 1 cut(s) 244
CviJI RGCY 8 cut(s) 34, 40, 53, 134, 200, 280, 341, 378
CviKI_1 RGCY 8 cut(s) 34, 40, 53, 134, 200, 280, 341, 378
CviQI GTAC 1 cut(s) 244
DdeI CTNAG 3 cut(s) 129, 168, 347
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
EciI GGCGGA 1 cut(s) 248
Eco130I CCWWGG 1 cut(s) 54
Eco147I AGGCCT 1 cut(s) 134
Eco57I CTGAAG 1 cut(s) 56
Eco88I CYCGRG 1 cut(s) 248
EcoNI CCTNNNNNAGG 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 377
EcoRI GAATTC 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 379
EcoT14I CCWWGG 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 54
FaiI YATR 2 cut(s) 216, 360
Fnu4HI GCNGC 3 cut(s) 181, 201, 204
Fsp4HI GCNGC 3 cut(s) 181, 201, 204
GlaI GCGC 1 cut(s) 109
GluI GCNGC 3 cut(s) 181, 201, 204
HaeIII GGCC 2 cut(s) 134, 378
HapII CCGG 1 cut(s) 230
HhaI GCGC 1 cut(s) 110
Hin1I GRCGYC 1 cut(s) 60
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HinfI GANTC 1 cut(s) 265
HpaII CCGG 1 cut(s) 230
HphI GGTGA 1 cut(s) 110
Hpy188I TCNGA 3 cut(s) 130, 225, 348
Hpy188III TCNNGA 1 cut(s) 320
HpyAV CCTTC 1 cut(s) 170
HpyCH4IV ACGT 2 cut(s) 18, 60
HpyCH4V TGCA 1 cut(s) 196
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 209
HpyF3I CTNAG 3 cut(s) 129, 168, 347
HpySE526I ACGT 2 cut(s) 18, 60
Hsp92I GRCGYC 1 cut(s) 60
HspAI GCGC 1 cut(s) 108
KpnI GGTACC 1 cut(s) 247
Kzo9I GATC 1 cut(s) 310
LpnPI CCDG 7 cut(s) 126, 182, 243, 287, 305, 323, 366
Lsp1109I GCAGC 2 cut(s) 187, 190
LweI GCATC 2 cut(s) 154, 183
MaeII ACGT 2 cut(s) 18, 60
MaeIII GTNAC 1 cut(s) 154
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 4 cut(s) 94, 103, 106, 109
MfeI CAATTG 1 cut(s) 122
MluCI AATT 2 cut(s) 122, 323
MnlI CCTC 6 cut(s) 124, 145, 231, 255, 257, 270
MseI TTAA 1 cut(s) 385
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 1 cut(s) 381
MunI CAATTG 1 cut(s) 122
MvaI CCWGG 1 cut(s) 381
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 2 cut(s) 186, 209
NdeII GATC 1 cut(s) 310
NlaIV GGNNCC 1 cut(s) 245
NmuCI GTSAC 1 cut(s) 154
NspV TTCGAA 1 cut(s) 80
PaeR7I CTCGAG 1 cut(s) 248
PceI AGGCCT 1 cut(s) 134
PfeI GAWTC 1 cut(s) 265
PkrI GCNGC 3 cut(s) 182, 202, 205
Ple19I CGATCG 1 cut(s) 313
Psp6I CCWGG 1 cut(s) 379
PspGI CCWGG 1 cut(s) 379
PspN4I GGNNCC 1 cut(s) 245
PspPI GGNCC 1 cut(s) 377
PspXI VCTCGAGB 1 cut(s) 248
PstNI CAGNNNCTG 1 cut(s) 337
PvuI CGATCG 1 cut(s) 313
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
SaqAI TTAA 1 cut(s) 385
SatI GCNGC 3 cut(s) 181, 201, 204
Sau3AI GATC 1 cut(s) 310
Sau96I GGNCC 1 cut(s) 377
ScrFI CCNGG 1 cut(s) 381
SetI ASST 6 cut(s) 21, 36, 42, 63, 145, 249
SfaNI GCATC 2 cut(s) 154, 183
Sfr274I CTCGAG 1 cut(s) 248
SfuI TTCGAA 1 cut(s) 80
SgrAI CRCCGGYG 1 cut(s) 229
SlaI CTCGAG 1 cut(s) 248
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 2 cut(s) 122, 323
SseBI AGGCCT 1 cut(s) 134
SsiI CCGC 5 cut(s) 69, 180, 233, 241, 307
StuI AGGCCT 1 cut(s) 134
StyD4I CCNGG 1 cut(s) 379
StyI CCWWGG 1 cut(s) 54
TaiI ACGT 2 cut(s) 21, 63
TaqI TCGA 4 cut(s) 12, 80, 249, 295
TasI AATT 2 cut(s) 122, 323
TauI GCSGC 1 cut(s) 183
TfiI GAWTC 1 cut(s) 265
Tru1I TTAA 1 cut(s) 385
Tru9I TTAA 1 cut(s) 385
TscAI CASTG 1 cut(s) 159
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 2 cut(s) 200, 203
Tsp45I GTSAC 1 cut(s) 154
TspRI CASTG 1 cut(s) 159
XagI CCTNNNNNAGG 1 cut(s) 372
XapI RAATTY 1 cut(s) 323
XhoI CTCGAG 1 cut(s) 248
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.