FvH4_2g30690

Eukaryotic family of unknown function (DUF1754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002762
Physical Location & Seq
Forward (+)
23718839 .. 23719499
661 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g30690.t1

Sequence Viewer

Length: 369 bp
ATGTCTGCGTACGATAACGTGGTGACTGGGAAGCTGAAGCTGAAAGGCAAAGCCTTGGACGTGAAAGGAGGGATCAAAAAGAAGAAGAAACACTACAAACGCTACGAGTATGATCAATCCATTCAAGTCTCAACAGGTGGAGATGCGATAGATCAATTTGAGAATGTTGATGATGACAATGGAAAGGGTGGAGTATTTGATCATATGACACCGGCGGAGAGACGATATATGGAGCAGGCAGAGAGGCTTCAAGCTCAAAGGCTGGCCAAGATGGCCAAGAAATCCCATCGCGATCGGGTTCAGGAATTCAATCAGTATCTGGCTAATCTGTCTGAGCATTATGACATCCCCAAAGTCGGCCCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1754

Protein Analysis

123

Amino Acids

13.92

Weight (kDa)

9.32

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM32A PF08555 3 - 85 1.3e-10 FAM32A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 291
AciI CCGC 1 cut(s) 215
AclWI GGATC 1 cut(s) 80
AcoI YGGCCR 2 cut(s) 264, 273
AcsI RAATTY 1 cut(s) 305
AcuI CTGAAG 1 cut(s) 56
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 1 cut(s) 356
AgsI TTSAA 3 cut(s) 125, 251, 310
AjiI CACGTC 1 cut(s) 61
AjnI CCWGG 1 cut(s) 361
AluBI AGCT 3 cut(s) 34, 40, 254
AluI AGCT 3 cut(s) 34, 40, 254
Alw26I GTCTC 2 cut(s) 133, 214
AlwI GGATC 1 cut(s) 80
AlwNI CAGNNNCTG 1 cut(s) 319
AoxI GGCC 3 cut(s) 264, 273, 358
ApoI RAATTY 1 cut(s) 305
AspS9I GGNCC 1 cut(s) 359
AsuHPI GGTGA 1 cut(s) 34
BalI TGGCCA 2 cut(s) 266, 275
BarI GAAGNNNNNNTAC 2 cut(s) 77, 109
BccI CCATC 2 cut(s) 265, 294
BciT130I CCWGG 1 cut(s) 363
BclI TGATCA 2 cut(s) 112, 199
BcoDI GTCTC 2 cut(s) 133, 214
BglI GCCNNNNNGGC 1 cut(s) 272
Bme1390I CCNGG 1 cut(s) 363
BmgBI CACGTC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 359
BmrFI CCNGG 1 cut(s) 363
BmrI ACTGGG 1 cut(s) 36
BmsI GCATC 1 cut(s) 133
BmuI ACTGGG 1 cut(s) 36
BsaJI CCNNGG 2 cut(s) 54, 361
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 1 cut(s) 31
BseBI CCWGG 1 cut(s) 363
BseDI CCNNGG 2 cut(s) 54, 361
BseGI GGATG 1 cut(s) 345
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 31
Bsh1236I CGCG 1 cut(s) 291
Bsh1285I CGRYCG 1 cut(s) 295
BshFI GGCC 3 cut(s) 266, 275, 360
BsiEI CGRYCG 1 cut(s) 295
BsiSI CCGG 1 cut(s) 212
BsiWI CGTACG 1 cut(s) 9
BslI CCNNNNNNNGG 1 cut(s) 356
BsmAI GTCTC 2 cut(s) 133, 214
BsmBI CGTCTC 1 cut(s) 214
BsnI GGCC 3 cut(s) 266, 275, 360
Bsp143I GATC 5 cut(s) 72, 112, 151, 199, 292
Bsp68I TCGCGA 1 cut(s) 291
BspACI CCGC 1 cut(s) 215
BspANI GGCC 3 cut(s) 266, 275, 360
BspCNI CTCAG 1 cut(s) 325
BspFNI CGCG 1 cut(s) 291
BspPI GGATC 1 cut(s) 80
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 1 cut(s) 31
BssAI RCCGGY 1 cut(s) 211
BssECI CCNNGG 2 cut(s) 54, 361
BssMI GATC 5 cut(s) 72, 112, 151, 199, 292
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 363
BstC8I GCNNGC 2 cut(s) 237, 264
BstDEI CTNAG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 345
BstFNI CGCG 1 cut(s) 291
BstKTI GATC 5 cut(s) 75, 115, 154, 202, 295
BstMAI GTCTC 2 cut(s) 133, 214
BstMBI GATC 5 cut(s) 72, 112, 151, 199, 292
BstMCI CGRYCG 1 cut(s) 295
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNI CCWGG 1 cut(s) 363
BstSCI CCNGG 1 cut(s) 361
BstUI CGCG 1 cut(s) 291
BsuRI GGCC 3 cut(s) 266, 275, 360
BtgZI GCGATG 1 cut(s) 272
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 1 cut(s) 345
BtuMI TCGCGA 1 cut(s) 291
Cac8I GCNNGC 2 cut(s) 237, 264
CaiI CAGNNNCTG 1 cut(s) 319
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 359
Csp6I GTAC 1 cut(s) 10
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 1 cut(s) 333
DpnI GATC 5 cut(s) 74, 114, 153, 201, 294
DpnII GATC 5 cut(s) 72, 112, 151, 199, 292
EaeI YGGCCR 2 cut(s) 264, 273
EciI GGCGGA 1 cut(s) 230
Eco130I CCWWGG 1 cut(s) 54
Eco57I CTGAAG 1 cut(s) 56
EcoRI GAATTC 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 361
EcoT14I CCWWGG 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 54
Esp3I CGTCTC 1 cut(s) 214
FaiI YATR 6 cut(s) 111, 204, 206, 228, 230, 342
FauNDI CATATG 1 cut(s) 204
FbaI TGATCA 2 cut(s) 112, 199
FokI GGATG 1 cut(s) 332
HaeIII GGCC 3 cut(s) 266, 275, 360
HapII CCGG 1 cut(s) 212
HpaII CCGG 1 cut(s) 212
HphI GGTGA 1 cut(s) 34
Hpy188I TCNGA 1 cut(s) 334
Hpy188III TCNNGA 2 cut(s) 290, 302
HpyCH4IV ACGT 2 cut(s) 18, 60
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 2 cut(s) 18, 60
Ksp22I TGATCA 2 cut(s) 112, 199
Kzo9I GATC 5 cut(s) 72, 112, 151, 199, 292
LmnI GCTCC 1 cut(s) 232
LpnPI CCDG 8 cut(s) 12, 120, 221, 225, 248, 287, 305, 348
LweI GCATC 1 cut(s) 133
MaeII ACGT 2 cut(s) 18, 60
MaeIII GTNAC 1 cut(s) 22
MalI GATC 5 cut(s) 74, 114, 153, 201, 294
MboI GATC 5 cut(s) 72, 112, 151, 199, 292
MboII GAAGA 2 cut(s) 94, 97
MlsI TGGCCA 2 cut(s) 266, 275
MluCI AATT 2 cut(s) 155, 305
MluNI TGGCCA 2 cut(s) 266, 275
MnlI CCTC 2 cut(s) 62, 237
Mox20I TGGCCA 2 cut(s) 266, 275
MscI TGGCCA 2 cut(s) 266, 275
MseI TTAA 1 cut(s) 367
Msp20I TGGCCA 2 cut(s) 266, 275
MspI CCGG 1 cut(s) 212
MspR9I CCNGG 1 cut(s) 363
MvaI CCWGG 1 cut(s) 363
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 272
NdeI CATATG 1 cut(s) 204
NdeII GATC 5 cut(s) 72, 112, 151, 199, 292
NmuCI GTSAC 1 cut(s) 22
NruI TCGCGA 1 cut(s) 291
Pfl23II CGTACG 1 cut(s) 9
Ple19I CGATCG 1 cut(s) 295
Psp6I CCWGG 1 cut(s) 361
PspGI CCWGG 1 cut(s) 361
PspLI CGTACG 1 cut(s) 9
PspPI GGNCC 1 cut(s) 359
PstNI CAGNNNCTG 1 cut(s) 319
PvuI CGATCG 1 cut(s) 295
RruI TCGCGA 1 cut(s) 291
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
SaqAI TTAA 1 cut(s) 367
Sau3AI GATC 5 cut(s) 72, 112, 151, 199, 292
Sau96I GGNCC 1 cut(s) 359
ScrFI CCNGG 1 cut(s) 363
SetI ASST 6 cut(s) 21, 36, 42, 63, 139, 256
SfaNI GCATC 1 cut(s) 133
SfiI GGCCNNNNNGGCC 1 cut(s) 272
SgrAI CRCCGGYG 1 cut(s) 211
Sse9I AATT 2 cut(s) 155, 305
SsiI CCGC 1 cut(s) 215
StyD4I CCNGG 1 cut(s) 361
StyI CCWWGG 1 cut(s) 54
TaiI ACGT 2 cut(s) 21, 63
TasI AATT 2 cut(s) 155, 305
Tru1I TTAA 1 cut(s) 367
Tru9I TTAA 1 cut(s) 367
TseFI GTSAC 1 cut(s) 22
Tsp45I GTSAC 1 cut(s) 22
XapI RAATTY 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.