Rorug02G0412200

Eukaryotic family of unknown function (DUF1754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002762
Physical Location & Seq
Reverse (-)
52817507 .. 52821585
4079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0412200.1

Sequence Viewer

Length: 1797 bp
ATGAGCTCAGAGGGGTTGAGTGCTTCACCACCGCCTCCCCAAGCGGTGATCGGAATGCAATACGGAGTGGCACATCCAATTTCGCTAGCCGGGCCAACTGAATTCGATATTGAGAGAACTTTAGAGTTGGAGAAGTTCTTGGTTGAAGCTGGGCTTTATGAAAGCAAAGAGCGGGAAGCTGCCAAGAGAGAGGAGGTTCTTCACCGAATTGGGCAGATTGTTAAGGATTGGGTGAAGCAACTTACTCAGTTGAGAGGATACACAGATCAAATGGTTGAGGATGCTAATGCTCTTATTTTTACATTCGGTTCATATAGGCTTGGGGTACATGGTCCTGGGGCTGAGATAGACACATTGTGTGTTGGGCCATCTTACGTCAACCGTGAGGAGGACTTCTTCTTTGTATTGCGCAACATTCTGGCTGAAATGGAAGAAGTGACTGAACTGCAACCAGTCCCAGATGCTCATGTTCCCGTAATGAAGTTTAAGTTCGATGGAATATCAATCGACCTCCTTTATGCCAGTGTTTCTCTGTTGGTTGTACCAGAAGACCTGGACATTTCTGATGTATCTCTGTTGTACAACATCGATGAGCCTACTGTTCGAAGTCTTAGTGGCTGCAGGGATGTTGATCAAATTCTTAAGCTTGTTCCAAATGTTGATCATTTCTGTACAACACTCCGATGCTTGAGGTTTTGGGCGAAAAGGCGTGGTGTTTATTCCAATGTTATTGGATTTCTTGGTGGTGTGAACTGGGCTCTCCTCGTTGCCCGAGTGTGTCAACTTTATCCTAATGCAGTTCCTAGCATGCTGGTTTCTCGATTTTTTAGAGTCTACACACAGTGGCGATGGCCAAATCCTGTCATGTTATGTGCCATTGAAGAGGATGAACTTGGTTTTGGTGTTTGGGACCCCCGCAAAAATCCTTGCGACCGGACTCATCATATGCCAGTTATAACTCCTGCATACCCTTGTATGAACTCTAGCTACAATGTTTCTAAAAGTACCCTCCGTGTCATGATGGAGCAATTTCAGCATGGAAACAAGATTTGTGAGGAAATACAACTGGATAAAGCCAGGTGGTGTACTCTGTTCGAACCATATTTGTTCTTTGAAAGCTACAAGAACTATCTCCAGGTCGACATCGTTGCAGTTGATGCTGATGATCTGCGTGCTTGGAAAGGCTGGGTGGAATCTCGGTTGAGGCAACTGACTCTAATGATTGAGCGGGATAATTTGGAGATGTTACAATGGCACCCATATCCTCATGAGTATGTTGATACAACCAAACAGTGTGCACATAGTGCTTTCTTTATGGGTTTTCAGAGGAAAAAAGGTGAAAAGCTTAATGGTCATCGATTCGATATACAAGGGACTGTTGATGAGTTCAAGCAGTCCATTGTTGATATGTACATTTTTGGGAAACCTGGGATGGAATTCTATGTTTCTCACGTTCGTAGAAGGCGCCTTCCTTCTTACGTGTTTCCTGATGGTTATAAACGACCTAGACGGTCTAGAGTTTTGGCCCAGCAACATGATCATGAAGTTAGTGCAACGAGTTCCGGTGAGAGACACCTGAAGAGGAAGAATGATCTTGGTGGGGGTTCGCCGGAGAAACGACTATCCATTATCCCTCACAAAGCAGGAAGTGCATCTCCAGAGACTTTGGATAAATGTGTTGGTATGGAGGTAGTTGAATCAAACTCTGGAGAACTGGAGCCTGGAGATGCCTCAAATTCCAGTGTTATTACAAATGTTACTAGCATCACTAGTGTTTTCGAAAAATTTGATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 map00100 map01100 map01110
Pfam Domains
Protein Families
DUF1754 ERG4_ERG24

Protein Analysis

598

Amino Acids

68.23

Weight (kDa)

5.78

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_NTPase PF20750 22 - 217 3.3e-70 Poly(A) polymerase nucleotidyltransferase domain
NTP_transf_2 PF01909 83 - 172 3.4e-10 Nucleotidyltransferase domain
PAP_central PF04928 222 - 366 3.3e-68 Poly(A) polymerase central domain
PAP_RNA-bind PF04926 370 - 428 1.4e-10 Poly(A) polymerase predicted RNA binding domain
PAP_RNA-bind PF04926 429 - 503 1.2e-06 Poly(A) polymerase predicted RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 956, 1497
Acc16I TGCGCA 1 cut(s) 410
AccB1I GGYRCC 2 cut(s) 1254, 1464
AccBSI CCGCTC 2 cut(s) 172, 1228
AccI GTMKAC 2 cut(s) 834, 1140
AciI CCGC 5 cut(s) 32, 44, 172, 916, 1228
AcoI YGGCCR 1 cut(s) 851
AcsI RAATTY 5 cut(s) 101, 636, 1436, 1735, 1784
AcuI CTGAAG 1 cut(s) 1598
AcyI GRCGYC 1 cut(s) 1465
AdeI CACNNNGTG 3 cut(s) 357, 843, 1304
AfaI GTAC 7 cut(s) 327, 543, 581, 673, 1006, 1087, 1412
AfiI CCNNNNNNNGG 1 cut(s) 388
AflII CTTAAG 1 cut(s) 641
AflIII ACRYGT 1 cut(s) 1479
AgsI TTSAA 5 cut(s) 146, 881, 1115, 1390, 1697
AhlI ACTAGT 1 cut(s) 1769
AjnI CCWGG 6 cut(s) 334, 552, 1076, 1134, 1426, 1720
AjuI GAANNNNNNNTTGG 2 cut(s) 882, 914
AluBI AGCT 7 cut(s) 6, 149, 179, 646, 987, 1119, 1345
AluI AGCT 7 cut(s) 6, 149, 179, 646, 987, 1119, 1345
Alw21I GWGCWC 2 cut(s) 8, 1300
Alw26I GTCTC 2 cut(s) 1564, 1655
Alw44I GTGCAC 1 cut(s) 1296
Ama87I CYCGRG 1 cut(s) 771
AoxI GGCC 4 cut(s) 92, 365, 851, 1524
ApaLI GTGCAC 1 cut(s) 1296
ApeKI GCWGC 2 cut(s) 179, 618
ApoI RAATTY 5 cut(s) 101, 636, 1436, 1735, 1784
ArsI GACNNNNNNTTYG 2 cut(s) 383, 415
AspLEI GCGC 2 cut(s) 411, 1467
AspS9I GGNCC 5 cut(s) 92, 332, 365, 910, 1525
AsuC2I CCSGG 1 cut(s) 91
AsuHPI GGTGA 6 cut(s) 18, 58, 194, 244, 1349, 1577
AsuII TTCGAA 3 cut(s) 604, 1095, 1779
AsuNHI GCTAGC 1 cut(s) 85
AvaI CYCGRG 1 cut(s) 771
AvaII GGWCC 2 cut(s) 332, 910
BaeGI GKGCMC 1 cut(s) 1300
BalI TGGCCA 1 cut(s) 853
BanI GGYRCC 2 cut(s) 1254, 1464
BanII GRGCYC 2 cut(s) 8, 760
BbsI GAAGAC 1 cut(s) 555
Bbv12I GWGCWC 2 cut(s) 8, 1300
BbvI GCAGC 2 cut(s) 166, 605
BccI CCATC 6 cut(s) 376, 488, 843, 1015, 1426, 1484
BcgI CGANNNNNNTGC 2 cut(s) 1130, 1164
BciT130I CCWGG 6 cut(s) 336, 554, 1078, 1136, 1428, 1722
BciVI GTATCC 1 cut(s) 251
BclI TGATCA 3 cut(s) 631, 661, 1537
BcnI CCSGG 1 cut(s) 91
BcoDI GTCTC 2 cut(s) 1564, 1655
BcuI ACTAGT 1 cut(s) 1769
BfaI CTAG 7 cut(s) 86, 804, 984, 1506, 1515, 1761, 1770
BfmI CTRYAG 1 cut(s) 619
BfoI RGCGCY 1 cut(s) 1468
BfrI CTTAAG 1 cut(s) 641
BfuI GTATCC 1 cut(s) 251
BisI GCNGC 2 cut(s) 180, 619
BlsI GCNGC 2 cut(s) 181, 620
Bme1390I CCNGG 7 cut(s) 91, 336, 554, 1078, 1136, 1428, 1722
Bme18I GGWCC 2 cut(s) 332, 910
BmeT110I CYCGRG 1 cut(s) 771
BmgT120I GGNCC 5 cut(s) 92, 332, 365, 910, 1525
BmiI GGNNCC 5 cut(s) 911, 912, 1256, 1466, 1719
BmrFI CCNGG 7 cut(s) 91, 336, 554, 1078, 1136, 1428, 1722
BmrI ACTGGG 1 cut(s) 763
BmsI GCATC 7 cut(s) 271, 451, 674, 1147, 1661, 1717, 1773
BmtI GCTAGC 1 cut(s) 89
BmuI ACTGGG 1 cut(s) 763
BpiI GAAGAC 1 cut(s) 555
BpmI CTGGAG 5 cut(s) 1118, 1641, 1728, 1736, 1743
Bpu14I TTCGAA 3 cut(s) 604, 1095, 1779
BpuEI CTTGAG 1 cut(s) 709
BpuMI CCSGG 1 cut(s) 91
Bsa29I ATCGAT 2 cut(s) 588, 1357
BsaAI YACGTR 1 cut(s) 1480
BsaBI GATNNNNATC 1 cut(s) 630
BsaHI GRCGYC 1 cut(s) 1465
BsaJI CCNNGG 2 cut(s) 335, 1427
BsaWI WCCGGW 2 cut(s) 933, 1562
Bsc4I CCNNNNNNNGG 1 cut(s) 388
Bse1I ACTGG 7 cut(s) 452, 522, 758, 950, 1071, 1719, 1740
Bse8I GATNNNNATC 1 cut(s) 630
BseBI CCWGG 6 cut(s) 336, 554, 1078, 1136, 1428, 1722
BseCI ATCGAT 2 cut(s) 588, 1357
BseDI CCNNGG 2 cut(s) 335, 1427
BseGI GGATG 5 cut(s) 73, 286, 631, 892, 1437
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 1 cut(s) 388
BseMII CTCAG 3 cut(s) 21, 260, 333
BseNI ACTGG 7 cut(s) 452, 522, 758, 950, 1071, 1719, 1740
BseRI GAGGAG 3 cut(s) 206, 401, 752
BseSI GKGCMC 1 cut(s) 1300
BseXI GCAGC 2 cut(s) 166, 605
BseYI CCCAGC 3 cut(s) 149, 1185, 1527
Bsh1285I CGRYCG 1 cut(s) 934
BshFI GGCC 4 cut(s) 94, 367, 853, 1526
BshNI GGYRCC 2 cut(s) 1254, 1464
BshVI ATCGAT 2 cut(s) 588, 1357
BsiEI CGRYCG 1 cut(s) 934
BsiHKAI GWGCWC 2 cut(s) 8, 1300
BsiHKCI CYCGRG 1 cut(s) 771
BsiSI CCGG 4 cut(s) 90, 934, 1563, 1610
BslFI GGGAC 3 cut(s) 440, 923, 1387
BslI CCNNNNNNNGG 1 cut(s) 388
BsmAI GTCTC 2 cut(s) 1564, 1655
BsmFI GGGAC 3 cut(s) 440, 923, 1387
BsmI GAATGC 1 cut(s) 60
BsnI GGCC 4 cut(s) 94, 367, 853, 1526
BsoBI CYCGRG 1 cut(s) 771
Bsp119I TTCGAA 3 cut(s) 604, 1095, 1779
Bsp1286I GDGCHC 3 cut(s) 8, 760, 1300
Bsp1407I TGTACA 3 cut(s) 579, 671, 1410
Bsp143I GATC 7 cut(s) 48, 265, 631, 661, 1165, 1537, 1591
BspACI CCGC 5 cut(s) 32, 44, 172, 916, 1228
BspANI GGCC 4 cut(s) 94, 367, 853, 1526
BspCNI CTCAG 3 cut(s) 20, 259, 334
BspDI ATCGAT 2 cut(s) 588, 1357
BspHI TCATGA 3 cut(s) 1017, 1267, 1540
BspLI GGNNCC 5 cut(s) 911, 912, 1256, 1466, 1719
BspMAI CTGCAG 1 cut(s) 623
BspOI GCTAGC 1 cut(s) 89
BspT104I TTCGAA 3 cut(s) 604, 1095, 1779
BspT107I GGYRCC 2 cut(s) 1254, 1464
BspTI CTTAAG 1 cut(s) 641
BsrBI CCGCTC 2 cut(s) 172, 1228
BsrGI TGTACA 3 cut(s) 579, 671, 1410
BsrI ACTGG 7 cut(s) 452, 522, 758, 950, 1071, 1719, 1740
BssECI CCNNGG 2 cut(s) 335, 1427
BssMI GATC 7 cut(s) 48, 265, 631, 661, 1165, 1537, 1591
BssNI GRCGYC 1 cut(s) 1465
Bst2UI CCWGG 6 cut(s) 336, 554, 1078, 1136, 1428, 1722
Bst4CI ACNGT 6 cut(s) 383, 601, 843, 1293, 1378, 1512
Bst6I CTCTTC 2 cut(s) 876, 1574
BstACI GRCGYC 1 cut(s) 1465
BstAFI CTTAAG 1 cut(s) 641
BstAPI GCANNNNNTGC 3 cut(s) 1157, 1304, 1649
BstAUI TGTACA 3 cut(s) 579, 671, 1410
BstBAI YACGTR 1 cut(s) 1480
BstBI TTCGAA 3 cut(s) 604, 1095, 1779
BstC8I GCNNGC 3 cut(s) 87, 809, 1173
BstDEI CTNAG 4 cut(s) 7, 246, 342, 611
BstF5I GGATG 5 cut(s) 73, 286, 631, 892, 1437
BstH2I RGCGCY 1 cut(s) 1468
BstHHI GCGC 2 cut(s) 411, 1467
BstKTI GATC 7 cut(s) 51, 268, 634, 664, 1168, 1540, 1594
BstMAI GTCTC 2 cut(s) 1564, 1655
BstMBI GATC 7 cut(s) 48, 265, 631, 661, 1165, 1537, 1591
BstMCI CGRYCG 1 cut(s) 934
BstMWI GCNNNNNNNGC 5 cut(s) 91, 1033, 1157, 1304, 1649
BstNI CCWGG 6 cut(s) 336, 554, 1078, 1136, 1428, 1722
BstNSI RCATGY 1 cut(s) 811
BstSCI CCNGG 7 cut(s) 89, 334, 552, 1076, 1134, 1426, 1720
BstSFI CTRYAG 1 cut(s) 619
BstSLI GKGCMC 1 cut(s) 1300
BstV1I GCAGC 2 cut(s) 166, 605
BstV2I GAAGAC 1 cut(s) 555
Bsu15I ATCGAT 2 cut(s) 588, 1357
BsuI GTATCC 1 cut(s) 251
BsuRI GGCC 4 cut(s) 94, 367, 853, 1526
BsuTUI ATCGAT 2 cut(s) 588, 1357
BtgZI GCGATG 1 cut(s) 862
BtsCI GGATG 5 cut(s) 73, 286, 631, 892, 1437
BtsIMutI CAGTG 4 cut(s) 529, 848, 1298, 1747
Cac8I GCNNGC 3 cut(s) 87, 809, 1173
CciI TCATGA 3 cut(s) 1017, 1267, 1540
CfoI GCGC 2 cut(s) 411, 1467
Cfr13I GGNCC 5 cut(s) 92, 332, 365, 910, 1525
ClaI ATCGAT 2 cut(s) 588, 1357
Csp6I GTAC 7 cut(s) 326, 542, 580, 672, 1005, 1086, 1411
CviAII CATG 9 cut(s) 329, 467, 808, 865, 1018, 1037, 1268, 1535, 1541
CviQI GTAC 7 cut(s) 326, 542, 580, 672, 1005, 1086, 1411
DdeI CTNAG 4 cut(s) 7, 246, 342, 611
DinI GGCGCC 1 cut(s) 1466
DpnI GATC 7 cut(s) 50, 267, 633, 663, 1167, 1539, 1593
DpnII GATC 7 cut(s) 48, 265, 631, 661, 1165, 1537, 1591
DraIII CACNNNGTG 3 cut(s) 357, 843, 1304
EaeI YGGCCR 1 cut(s) 851
Eam1104I CTCTTC 2 cut(s) 876, 1574
EarI CTCTTC 2 cut(s) 876, 1574
Ecl136II GAGCTC 1 cut(s) 6
Eco24I GRGCYC 2 cut(s) 8, 760
Eco47I GGWCC 2 cut(s) 332, 910
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 1598
Eco88I CYCGRG 1 cut(s) 771
EcoICRI GAGCTC 1 cut(s) 6
EcoO109I RGGNCCY 1 cut(s) 910
EcoRI GAATTC 2 cut(s) 101, 1436
EcoRII CCWGG 6 cut(s) 334, 552, 1076, 1134, 1426, 1720
EcoT38I GRGCYC 2 cut(s) 8, 760
EgeI GGCGCC 1 cut(s) 1466
EheI GGCGCC 1 cut(s) 1466
FaeI CATG 9 cut(s) 332, 470, 811, 868, 1021, 1040, 1271, 1538, 1544
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FaqI GGGAC 3 cut(s) 440, 923, 1387
FatI CATG 9 cut(s) 328, 466, 807, 864, 1017, 1036, 1267, 1534, 1540
FauI CCCGC 3 cut(s) 165, 923, 1221
FauNDI CATATG 1 cut(s) 945
FbaI TGATCA 3 cut(s) 631, 661, 1537
FblI GTMKAC 2 cut(s) 834, 1140
Fnu4HI GCNGC 2 cut(s) 180, 619
FokI GGATG 5 cut(s) 60, 293, 638, 899, 1444
FriOI GRGCYC 2 cut(s) 8, 760
Fsp4HI GCNGC 2 cut(s) 180, 619
FspBI CTAG 7 cut(s) 86, 804, 984, 1506, 1515, 1761, 1770
FspI TGCGCA 1 cut(s) 410
GlaI GCGC 2 cut(s) 410, 1466
GluI GCNGC 2 cut(s) 180, 619
GsaI CCCAGC 3 cut(s) 153, 1189, 1531
GsuI CTGGAG 5 cut(s) 1118, 1641, 1728, 1736, 1743
HaeII RGCGCY 1 cut(s) 1468
HaeIII GGCC 4 cut(s) 94, 367, 853, 1526
HapII CCGG 4 cut(s) 90, 934, 1563, 1610
HhaI GCGC 2 cut(s) 411, 1467
Hin1I GRCGYC 1 cut(s) 1465
Hin1II CATG 9 cut(s) 332, 470, 811, 868, 1021, 1040, 1271, 1538, 1544
Hin6I GCGC 2 cut(s) 409, 1465
HinP1I GCGC 2 cut(s) 409, 1465
HincII GTYRAC 3 cut(s) 379, 782, 1141
HindII GTYRAC 3 cut(s) 379, 782, 1141
HindIII AAGCTT 2 cut(s) 644, 1343
HinfI GANTC 6 cut(s) 831, 937, 1193, 1213, 1359, 1697
HpaII CCGG 4 cut(s) 90, 934, 1563, 1610
HphI GGTGA 6 cut(s) 18, 58, 194, 244, 1349, 1577
Hpy166II GTNNAC 7 cut(s) 379, 751, 782, 835, 1086, 1141, 1298
Hpy188I TCNGA 5 cut(s) 10, 53, 565, 683, 1326
Hpy188III TCNNGA 8 cut(s) 819, 1018, 1268, 1487, 1515, 1541, 1658, 1707
Hpy8I GTNNAC 7 cut(s) 379, 751, 782, 835, 1086, 1141, 1298
HpyAV CCTTC 3 cut(s) 1455, 1478, 1482
HpyCH4III ACNGT 6 cut(s) 383, 601, 843, 1293, 1378, 1512
HpyCH4IV ACGT 3 cut(s) 375, 1452, 1479
HpyCH4V TGCA 9 cut(s) 58, 448, 621, 797, 965, 1151, 1298, 1553, 1652
HpyF10VI GCNNNNNNNGC 5 cut(s) 91, 1033, 1157, 1304, 1649
HpyF3I CTNAG 4 cut(s) 7, 246, 342, 611
HpySE526I ACGT 3 cut(s) 375, 1452, 1479
Hsp92I GRCGYC 1 cut(s) 1465
Hsp92II CATG 9 cut(s) 332, 470, 811, 868, 1021, 1040, 1271, 1538, 1544
HspAI GCGC 2 cut(s) 409, 1465
KasI GGCGCC 1 cut(s) 1464
KflI GGGWCCC 1 cut(s) 910
Ksp22I TGATCA 3 cut(s) 631, 661, 1537
Kzo9I GATC 7 cut(s) 48, 265, 631, 661, 1165, 1537, 1591
LmnI GCTCC 2 cut(s) 1024, 1717
Lsp1109I GCAGC 2 cut(s) 166, 605
LweI GCATC 7 cut(s) 271, 451, 674, 1147, 1661, 1717, 1773
MaeI CTAG 7 cut(s) 86, 804, 984, 1506, 1515, 1761, 1770
MaeII ACGT 3 cut(s) 375, 1452, 1479
MaeIII GTNAC 3 cut(s) 436, 1245, 1756
MalI GATC 7 cut(s) 50, 267, 633, 663, 1167, 1539, 1593
MbiI CCGCTC 2 cut(s) 172, 1228
MboI GATC 7 cut(s) 48, 265, 631, 661, 1165, 1537, 1591
MboII GAAGA 7 cut(s) 191, 388, 443, 560, 893, 1591, 1597
MhlI GDGCHC 3 cut(s) 8, 760, 1300
MlsI TGGCCA 1 cut(s) 853
MluCI AATT 9 cut(s) 78, 101, 207, 636, 1028, 1234, 1436, 1735, 1784
MluNI TGGCCA 1 cut(s) 853
Mly113I GGCGCC 1 cut(s) 1465
MlyI GAGTC 3 cut(s) 840, 931, 1207
MmeI TCCRAC 1 cut(s) 108
Mox20I TGGCCA 1 cut(s) 853
MscI TGGCCA 1 cut(s) 853
MseI TTAA 4 cut(s) 222, 486, 642, 1347
MslI CAYNNNNRTG 3 cut(s) 682, 1272, 1539
Msp20I TGGCCA 1 cut(s) 853
MspCI CTTAAG 1 cut(s) 641
MspI CCGG 4 cut(s) 90, 934, 1563, 1610
MspR9I CCNGG 7 cut(s) 91, 336, 554, 1078, 1136, 1428, 1722
Mva1269I GAATGC 1 cut(s) 60
MvaI CCWGG 6 cut(s) 336, 554, 1078, 1136, 1428, 1722
MwoI GCNNNNNNNGC 5 cut(s) 91, 1033, 1157, 1304, 1649
NarI GGCGCC 1 cut(s) 1465
NciI CCSGG 1 cut(s) 91
NdeI CATATG 1 cut(s) 945
NdeII GATC 7 cut(s) 48, 265, 631, 661, 1165, 1537, 1591
NheI GCTAGC 1 cut(s) 85
NlaIII CATG 9 cut(s) 332, 470, 811, 868, 1021, 1040, 1271, 1538, 1544
NlaIV GGNNCC 5 cut(s) 911, 912, 1256, 1466, 1719
NmuCI GTSAC 1 cut(s) 436
NsbI TGCGCA 1 cut(s) 410
NspI RCATGY 1 cut(s) 811
NspV TTCGAA 3 cut(s) 604, 1095, 1779
PaeI GCATGC 1 cut(s) 811
PagI TCATGA 3 cut(s) 1017, 1267, 1540
PctI GAATGC 1 cut(s) 60
PfeI GAWTC 3 cut(s) 1193, 1359, 1697
PkrI GCNGC 2 cut(s) 181, 620
PleI GAGTC 3 cut(s) 839, 931, 1207
PluTI GGCGCC 1 cut(s) 1468
PpsI GAGTC 3 cut(s) 839, 931, 1207
Ppu21I YACGTR 1 cut(s) 1480
PpuMI RGGWCCY 1 cut(s) 910
PsiI TTATAA 2 cut(s) 956, 1497
Psp124BI GAGCTC 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 910
Psp6I CCWGG 6 cut(s) 334, 552, 1076, 1134, 1426, 1720
PspFI CCCAGC 3 cut(s) 149, 1185, 1527
PspGI CCWGG 6 cut(s) 334, 552, 1076, 1134, 1426, 1720
PspN4I GGNNCC 5 cut(s) 911, 912, 1256, 1466, 1719
PspPI GGNCC 5 cut(s) 92, 332, 365, 910, 1525
PspPPI RGGWCCY 1 cut(s) 910
PsrI GAACNNNNNNTAC 2 cut(s) 971, 1003
PstI CTGCAG 1 cut(s) 623
RsaI GTAC 7 cut(s) 327, 543, 581, 673, 1006, 1087, 1412
RsaNI GTAC 7 cut(s) 326, 542, 580, 672, 1005, 1086, 1411
RseI CAYNNNNRTG 3 cut(s) 682, 1272, 1539
SacI GAGCTC 1 cut(s) 8
SalI GTCGAC 1 cut(s) 1139
SaqAI TTAA 4 cut(s) 222, 486, 642, 1347
SatI GCNGC 2 cut(s) 180, 619
Sau3AI GATC 7 cut(s) 48, 265, 631, 661, 1165, 1537, 1591
Sau96I GGNCC 5 cut(s) 92, 332, 365, 910, 1525
SchI GAGTC 3 cut(s) 840, 931, 1207
ScrFI CCNGG 7 cut(s) 91, 336, 554, 1078, 1136, 1428, 1722
SduI GDGCHC 3 cut(s) 8, 760, 1300
SfaNI GCATC 7 cut(s) 271, 451, 674, 1147, 1661, 1717, 1773
SfcI CTRYAG 1 cut(s) 619
SfoI GGCGCC 1 cut(s) 1466
SfuI TTCGAA 3 cut(s) 604, 1095, 1779
SinI GGWCC 2 cut(s) 332, 910
SmiMI CAYNNNNRTG 3 cut(s) 682, 1272, 1539
SmlI CTYRAG 2 cut(s) 641, 688
SmoI CTYRAG 2 cut(s) 641, 688
SpeI ACTAGT 1 cut(s) 1769
SphI GCATGC 1 cut(s) 811
Sse9I AATT 9 cut(s) 78, 101, 207, 636, 1028, 1234, 1436, 1735, 1784
SsiI CCGC 5 cut(s) 32, 44, 172, 916, 1228
SspDI GGCGCC 1 cut(s) 1464
SspMI CTAG 7 cut(s) 86, 804, 984, 1506, 1515, 1761, 1770
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 7 cut(s) 89, 334, 552, 1076, 1134, 1426, 1720
TaaI ACNGT 6 cut(s) 383, 601, 843, 1293, 1378, 1512
TaiI ACGT 3 cut(s) 378, 1455, 1482
TasI AATT 9 cut(s) 78, 101, 207, 636, 1028, 1234, 1436, 1735, 1784
TatI WGTACW 4 cut(s) 579, 671, 1085, 1410
TfiI GAWTC 3 cut(s) 1193, 1359, 1697
Tru1I TTAA 4 cut(s) 222, 486, 642, 1347
Tru9I TTAA 4 cut(s) 222, 486, 642, 1347
TscAI CASTG 4 cut(s) 529, 848, 1298, 1747
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 2 cut(s) 179, 618
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 6 cut(s) 174, 300, 494, 903, 992, 1557
TspGWI ACGGA 2 cut(s) 78, 1001
TspRI CASTG 4 cut(s) 529, 848, 1298, 1747
Vha464I CTTAAG 1 cut(s) 641
VneI GTGCAC 1 cut(s) 1296
VpaK11BI GGWCC 2 cut(s) 332, 910
XapI RAATTY 5 cut(s) 101, 636, 1436, 1735, 1784
XbaI TCTAGA 1 cut(s) 1514
XceI RCATGY 1 cut(s) 811
XmiI GTMKAC 2 cut(s) 834, 1140
XspI CTAG 7 cut(s) 86, 804, 984, 1506, 1515, 1761, 1770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.