Rroxscaffold_3G00254310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002762
Physical Location & Seq
Reverse (-)
48439413 .. 48466848
27436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00254310.1

Sequence Viewer

Length: 402 bp
ATGGGGATGCGGGGAGGCCGCGGGGTGGTGTTGATGGAGCTCACCGGCGGCGAGAACGGTTTGCGGTATGGTTGGATTACCGTTGGGGGAAGTGGGCATGCCCGTATATGTAAGACACTAGCTACATACATATATCGAATCTTTTTGGATGAGGGTCCAGTAATAGTGCATGATTTTGAAGATCCAATGCATCTTCCATCTTATGGACAGTCCATAACTGTAGGATTTGTTAGTCAACTCTATCTGACTACCACGGGGAATCCCGCAGGCCGGAGAACCAGGGAGGTTTTTCGAGAGTTTTTATTCGAGTGGTGTGAGGTGTGTTTATTTTCGAGAACAAAATTCAGAACGGACTTCAAAGTTGAAGATGAAGTGCACGAGGATGCTGCAGATTTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

133

Amino Acids

15.09

Weight (kDa)

5.34

Isoelectric Point (pI)

25.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 203
AccII CGCG 1 cut(s) 21
AciI CCGC 6 cut(s) 10, 19, 21, 48, 64, 264
AclWI GGATC 1 cut(s) 176
AcsI RAATTY 1 cut(s) 341
AfiI CCNNNNNNNGG 3 cut(s) 25, 203, 270
AgsI TTSAA 3 cut(s) 179, 358, 365
AjnI CCWGG 1 cut(s) 278
AluBI AGCT 2 cut(s) 40, 122
AluI AGCT 2 cut(s) 40, 122
Alw21I GWGCWC 2 cut(s) 42, 378
Alw44I GTGCAC 1 cut(s) 374
AlwI GGATC 1 cut(s) 176
AoxI GGCC 2 cut(s) 16, 268
ApaLI GTGCAC 1 cut(s) 374
ApeKI GCWGC 1 cut(s) 386
ApoI RAATTY 1 cut(s) 341
AspS9I GGNCC 1 cut(s) 155
AsuHPI GGTGA 1 cut(s) 34
AvaII GGWCC 1 cut(s) 155
BaeGI GKGCMC 1 cut(s) 378
BanII GRGCYC 1 cut(s) 42
BauI CACGAG 1 cut(s) 377
Bbv12I GWGCWC 2 cut(s) 42, 378
BbvI GCAGC 1 cut(s) 373
BccI CCATC 2 cut(s) 28, 205
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciT130I CCWGG 1 cut(s) 280
BfaI CTAG 1 cut(s) 119
BfmI CTRYAG 2 cut(s) 219, 387
BisI GCNGC 3 cut(s) 19, 49, 387
BlsI GCNGC 3 cut(s) 20, 50, 388
Bme1390I CCNGG 1 cut(s) 280
Bme18I GGWCC 1 cut(s) 155
BmgT120I GGNCC 1 cut(s) 155
BmiI GGNNCC 1 cut(s) 156
BmrFI CCNGG 1 cut(s) 280
BmsI GCATC 2 cut(s) 199, 373
BsaJI CCNNGG 3 cut(s) 19, 252, 279
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 203, 270
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 1 cut(s) 158
BseBI CCWGG 1 cut(s) 280
BseDI CCNNGG 3 cut(s) 19, 252, 279
BseGI GGATG 3 cut(s) 12, 154, 388
BseLI CCNNNNNNNGG 3 cut(s) 25, 203, 270
BseNI ACTGG 1 cut(s) 158
BseSI GKGCMC 1 cut(s) 378
BseXI GCAGC 1 cut(s) 373
Bsh1236I CGCG 1 cut(s) 21
BshFI GGCC 2 cut(s) 18, 270
BsiHKAI GWGCWC 2 cut(s) 42, 378
BsiSI CCGG 2 cut(s) 45, 271
BslI CCNNNNNNNGG 3 cut(s) 25, 203, 270
BsnI GGCC 2 cut(s) 18, 270
Bsp1286I GDGCHC 2 cut(s) 42, 378
Bsp143I GATC 1 cut(s) 181
BspACI CCGC 6 cut(s) 10, 19, 21, 48, 64, 264
BspANI GGCC 2 cut(s) 18, 270
BspFNI CGCG 1 cut(s) 21
BspLI GGNNCC 1 cut(s) 156
BspMAI CTGCAG 1 cut(s) 391
BspPI GGATC 1 cut(s) 176
BsrFI RCCGGY 1 cut(s) 44
BsrI ACTGG 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 44
BssECI CCNNGG 3 cut(s) 19, 252, 279
BssMI GATC 1 cut(s) 181
BssSI CACGAG 1 cut(s) 377
Bst2BI CACGAG 1 cut(s) 377
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 4 cut(s) 59, 82, 210, 220
BstC8I GCNNGC 2 cut(s) 99, 268
BstDSI CCRYGG 2 cut(s) 19, 252
BstF5I GGATG 3 cut(s) 12, 154, 388
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 1 cut(s) 184
BstMBI GATC 1 cut(s) 181
BstNI CCWGG 1 cut(s) 280
BstNSI RCATGY 1 cut(s) 101
BstSCI CCNGG 1 cut(s) 278
BstSFI CTRYAG 2 cut(s) 219, 387
BstSLI GKGCMC 1 cut(s) 378
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 1 cut(s) 373
BstX2I RGATCY 1 cut(s) 181
BstYI RGATCY 1 cut(s) 181
BsuRI GGCC 2 cut(s) 18, 270
BtgI CCRYGG 2 cut(s) 19, 252
BtsCI GGATG 3 cut(s) 12, 154, 388
Cac8I GCNNGC 2 cut(s) 99, 268
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 155
Cfr42I CCGCGG 1 cut(s) 22
CviAII CATG 2 cut(s) 98, 170
CviJI RGCY 4 cut(s) 18, 40, 122, 270
CviKI_1 RGCY 4 cut(s) 18, 40, 122, 270
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
Ecl136II GAGCTC 1 cut(s) 40
Eco24I GRGCYC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 155
Eco53kI GAGCTC 1 cut(s) 40
EcoICRI GAGCTC 1 cut(s) 40
EcoRII CCWGG 1 cut(s) 278
EcoT22I ATGCAT 1 cut(s) 192
EcoT38I GRGCYC 1 cut(s) 42
FaeI CATG 2 cut(s) 101, 173
FatI CATG 2 cut(s) 97, 169
FauI CCCGC 3 cut(s) 3, 14, 271
Fnu4HI GCNGC 3 cut(s) 19, 49, 387
FokI GGATG 3 cut(s) 19, 161, 395
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 3 cut(s) 19, 49, 387
FspBI CTAG 1 cut(s) 119
GluI GCNGC 3 cut(s) 19, 49, 387
HaeIII GGCC 2 cut(s) 18, 270
HapII CCGG 2 cut(s) 45, 271
Hin1II CATG 2 cut(s) 101, 173
HincII GTYRAC 1 cut(s) 236
HindII GTYRAC 1 cut(s) 236
HinfI GANTC 2 cut(s) 138, 259
HpaII CCGG 2 cut(s) 45, 271
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 2 cut(s) 236, 376
Hpy188I TCNGA 2 cut(s) 246, 347
Hpy188III TCNNGA 2 cut(s) 293, 333
Hpy8I GTNNAC 2 cut(s) 236, 376
HpyCH4III ACNGT 4 cut(s) 59, 82, 210, 220
HpyCH4V TGCA 4 cut(s) 169, 190, 376, 389
Hsp92II CATG 2 cut(s) 101, 173
KspI CCGCGG 1 cut(s) 22
Kzo9I GATC 1 cut(s) 181
LmnI GCTCC 1 cut(s) 37
LpnPI CCDG 6 cut(s) 58, 171, 252, 265, 284, 292
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 2 cut(s) 199, 373
MaeI CTAG 1 cut(s) 119
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 3 cut(s) 185, 191, 377
MflI RGATCY 1 cut(s) 181
MhlI GDGCHC 2 cut(s) 42, 378
MluCI AATT 1 cut(s) 341
MmeI TCCRAC 1 cut(s) 53
MnlI CCTC 5 cut(s) 8, 145, 277, 310, 373
Mph1103I ATGCAT 1 cut(s) 192
MslI CAYNNNNRTG 1 cut(s) 381
MspA1I CMGCKG 1 cut(s) 21
MspI CCGG 2 cut(s) 45, 271
MspR9I CCNGG 1 cut(s) 280
MvaI CCWGG 1 cut(s) 280
MvnI CGCG 1 cut(s) 21
NdeII GATC 1 cut(s) 181
NlaIII CATG 2 cut(s) 101, 173
NlaIV GGNNCC 1 cut(s) 156
NsiI ATGCAT 1 cut(s) 192
NspI RCATGY 1 cut(s) 101
PaeI GCATGC 1 cut(s) 101
PfeI GAWTC 2 cut(s) 138, 259
PflMI CCANNNNNTGG 1 cut(s) 203
PkrI GCNGC 3 cut(s) 20, 50, 388
Psp124BI GAGCTC 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 278
PspGI CCWGG 1 cut(s) 278
PspN4I GGNNCC 1 cut(s) 156
PspPI GGNCC 1 cut(s) 155
PstI CTGCAG 1 cut(s) 391
PsuI RGATCY 1 cut(s) 181
RseI CAYNNNNRTG 1 cut(s) 381
SacI GAGCTC 1 cut(s) 42
SacII CCGCGG 1 cut(s) 22
SatI GCNGC 3 cut(s) 19, 49, 387
Sau3AI GATC 1 cut(s) 181
Sau96I GGNCC 1 cut(s) 155
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 2 cut(s) 42, 378
SetI ASST 4 cut(s) 42, 124, 288, 321
SfaNI GCATC 2 cut(s) 199, 373
SfcI CTRYAG 2 cut(s) 219, 387
Sfr303I CCGCGG 1 cut(s) 22
SgrAI CRCCGGYG 1 cut(s) 44
SgrBI CCGCGG 1 cut(s) 22
SinI GGWCC 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 381
SphI GCATGC 1 cut(s) 101
Sse9I AATT 1 cut(s) 341
SsiI CCGC 6 cut(s) 10, 19, 21, 48, 64, 264
SspMI CTAG 1 cut(s) 119
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 278
TaaI ACNGT 4 cut(s) 59, 82, 210, 220
TaqI TCGA 4 cut(s) 136, 292, 306, 332
TasI AATT 1 cut(s) 341
TauI GCSGC 2 cut(s) 21, 51
TfiI GAWTC 2 cut(s) 138, 259
TseI GCWGC 1 cut(s) 386
TspDTI ATGAA 1 cut(s) 384
TspGWI ACGGA 1 cut(s) 365
Van91I CCANNNNNTGG 1 cut(s) 203
VneI GTGCAC 1 cut(s) 374
VpaK11BI GGWCC 1 cut(s) 155
XapI RAATTY 1 cut(s) 341
XceI RCATGY 1 cut(s) 101
XspI CTAG 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.