MD09G1165100.v1.1

Eukaryotic family of unknown function (DUF1754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002762
Physical Location & Seq
Forward (+)
13519275 .. 13521303
2029 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1165100.v1.1.491

Sequence Viewer

Length: 507 bp
ATGATAAAACATTCTTCTGTGAAATTCCAAAGCGATCGAGATCAAGCACTCCTTCTCTTTTGTTTCAGGAAATTATTTGTTTTAGACACAAACATGTCGTCGTATGAGAATGTTGTTGGTGGGAAGCTGAAGCTCAAGGGAAAGGCTCTGGATGTGAAGGCTGCTGGAATGAAGAAGAAAAAGAAGCATAAGAAACATCAAGAACAAACTTCTAGTGTAACGGAAACTGAGCTCTCAGCTGATGTAGATGGAAGTACAGAAGTAACCAATCCCGATGAGGAAGAAATAAACGATTCGCACAAATCAGGTGAGGAGGGGAAGGCTCCTCACTACGGTGATCTTCTCACACCTGCAGAGAGACGATATATAGAACAGAGAGAGAGAATTGATAATCACAGGTTGGCCAAGACAGCAAACAAGTCCCACCGTGACAGAATTCAAGACTTCAACCAATATCTGGCAAACATGAGCGAGCATTATGACATTCCTAAAGTCGGGCCAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1754

Protein Analysis

169

Amino Acids

19.13

Weight (kDa)

8.52

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM32A PF08555 35 - 131 4e-16 FAM32A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 358
Acc36I ACCTGC 1 cut(s) 358
AccB7I CCANNNNNTGG 1 cut(s) 457
AcoI YGGCCR 1 cut(s) 402
AcsI RAATTY 2 cut(s) 23, 435
AcuI CTGAAG 1 cut(s) 149
AfaI GTAC 1 cut(s) 256
AfiI CCNNNNNNNGG 3 cut(s) 332, 457, 494
AflIII ACRYGT 1 cut(s) 93
AgsI TTSAA 2 cut(s) 440, 448
AjnI CCWGG 1 cut(s) 499
AleI CACNNNNGTG 1 cut(s) 333
AluBI AGCT 4 cut(s) 127, 133, 232, 239
AluI AGCT 4 cut(s) 127, 133, 232, 239
Alw21I GWGCWC 1 cut(s) 234
Alw26I GTCTC 1 cut(s) 352
AoxI GGCC 2 cut(s) 402, 497
ApeKI GCWGC 1 cut(s) 161
ApoI RAATTY 2 cut(s) 23, 435
ArsI GACNNNNNNTTYG 2 cut(s) 83, 115
AspS9I GGNCC 1 cut(s) 497
AsuHPI GGTGA 2 cut(s) 320, 347
BalI TGGCCA 1 cut(s) 404
BanII GRGCYC 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 1 cut(s) 148
BccI CCATC 1 cut(s) 242
BciT130I CCWGG 1 cut(s) 501
BcoDI GTCTC 1 cut(s) 352
BfaI CTAG 1 cut(s) 213
BfmI CTRYAG 1 cut(s) 351
BfuAI ACCTGC 1 cut(s) 358
BisI GCNGC 1 cut(s) 162
BlsI GCNGC 1 cut(s) 163
Bme1390I CCNGG 1 cut(s) 501
BmgT120I GGNCC 1 cut(s) 497
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 1 cut(s) 501
BpuEI CTTGAG 1 cut(s) 119
BsaBI GATNNNNATC 1 cut(s) 39
Bsc4I CCNNNNNNNGG 3 cut(s) 332, 457, 494
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 1 cut(s) 501
BseGI GGATG 1 cut(s) 157
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 3 cut(s) 332, 457, 494
BseMII CTCAG 2 cut(s) 219, 249
BseRI GAGGAG 2 cut(s) 315, 326
BseXI GCAGC 1 cut(s) 148
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 2 cut(s) 404, 499
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 234
BslFI GGGAC 1 cut(s) 406
BslI CCNNNNNNNGG 3 cut(s) 332, 457, 494
BsmAI GTCTC 1 cut(s) 352
BsmBI CGTCTC 1 cut(s) 352
BsmFI GGGAC 1 cut(s) 406
BsnI GGCC 2 cut(s) 404, 499
Bsp1286I GDGCHC 1 cut(s) 234
Bsp143I GATC 3 cut(s) 34, 40, 337
BspANI GGCC 2 cut(s) 404, 499
BspCNI CTCAG 2 cut(s) 220, 248
BspLI GGNNCC 1 cut(s) 324
BspMAI CTGCAG 1 cut(s) 355
BspMI ACCTGC 1 cut(s) 358
BssMI GATC 3 cut(s) 34, 40, 337
Bst2UI CCWGG 1 cut(s) 501
Bst4CI ACNGT 2 cut(s) 335, 428
BstC8I GCNNGC 1 cut(s) 473
BstDEI CTNAG 2 cut(s) 228, 235
BstF5I GGATG 1 cut(s) 157
BstKTI GATC 3 cut(s) 37, 43, 340
BstMAI GTCTC 1 cut(s) 352
BstMBI GATC 3 cut(s) 34, 40, 337
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 1 cut(s) 410
BstNI CCWGG 1 cut(s) 501
BstNSI RCATGY 1 cut(s) 97
BstSCI CCNGG 1 cut(s) 499
BstSFI CTRYAG 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 148
BsuRI GGCC 2 cut(s) 404, 499
BtsCI GGATG 1 cut(s) 157
BveI ACCTGC 1 cut(s) 358
Cac8I GCNNGC 1 cut(s) 473
Cfr13I GGNCC 1 cut(s) 497
Csp6I GTAC 1 cut(s) 255
CviAII CATG 2 cut(s) 94, 466
CviQI GTAC 1 cut(s) 255
DdeI CTNAG 2 cut(s) 228, 235
DpnI GATC 3 cut(s) 36, 42, 339
DpnII GATC 3 cut(s) 34, 40, 337
EaeI YGGCCR 1 cut(s) 402
Ecl136II GAGCTC 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 234
Eco53kI GAGCTC 1 cut(s) 232
Eco57I CTGAAG 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 232
EcoRI GAATTC 1 cut(s) 435
EcoRII CCWGG 1 cut(s) 499
EcoT38I GRGCYC 1 cut(s) 234
Esp3I CGTCTC 1 cut(s) 352
FaeI CATG 2 cut(s) 97, 469
FaiI YATR 7 cut(s) 95, 105, 189, 366, 368, 467, 480
FalI AAGNNNNNCTT 2 cut(s) 36, 68
FaqI GGGAC 1 cut(s) 406
FatI CATG 2 cut(s) 93, 465
Fnu4HI GCNGC 1 cut(s) 162
FokI GGATG 1 cut(s) 164
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 1 cut(s) 162
FspBI CTAG 1 cut(s) 213
GluI GCNGC 1 cut(s) 162
HaeIII GGCC 2 cut(s) 404, 499
Hin1II CATG 2 cut(s) 97, 469
HinfI GANTC 1 cut(s) 293
HphI GGTGA 2 cut(s) 320, 347
Hpy188III TCNNGA 6 cut(s) 38, 67, 149, 200, 272, 440
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 3 cut(s) 62, 151, 313
HpyCH4III ACNGT 2 cut(s) 335, 428
HpyCH4V TGCA 1 cut(s) 353
HpyF10VI GCNNNNNNNGC 1 cut(s) 410
HpyF3I CTNAG 2 cut(s) 228, 235
Hsp92II CATG 2 cut(s) 97, 469
Kzo9I GATC 3 cut(s) 34, 40, 337
LmnI GCTCC 1 cut(s) 328
LpnPI CCDG 8 cut(s) 52, 134, 150, 291, 363, 382, 443, 486
Lsp1109I GCAGC 1 cut(s) 148
MaeI CTAG 1 cut(s) 213
MaeIII GTNAC 3 cut(s) 217, 262, 428
MalI GATC 3 cut(s) 36, 42, 339
MboI GATC 3 cut(s) 34, 40, 337
MboII GAAGA 5 cut(s) 6, 184, 187, 293, 332
MhlI GDGCHC 1 cut(s) 234
MlsI TGGCCA 1 cut(s) 404
MluCI AATT 4 cut(s) 23, 71, 384, 435
MluNI TGGCCA 1 cut(s) 404
MnlI CCTC 4 cut(s) 271, 304, 307, 336
Mox20I TGGCCA 1 cut(s) 404
MscI TGGCCA 1 cut(s) 404
MslI CAYNNNNRTG 2 cut(s) 92, 333
Msp20I TGGCCA 1 cut(s) 404
MspA1I CMGCKG 1 cut(s) 239
MspR9I CCNGG 1 cut(s) 501
MvaI CCWGG 1 cut(s) 501
MwoI GCNNNNNNNGC 1 cut(s) 410
NdeII GATC 3 cut(s) 34, 40, 337
NlaIII CATG 2 cut(s) 97, 469
NlaIV GGNNCC 1 cut(s) 324
NmuCI GTSAC 1 cut(s) 428
NspI RCATGY 1 cut(s) 97
OliI CACNNNNGTG 1 cut(s) 333
PaqCI CACCTGC 1 cut(s) 358
PciI ACATGT 1 cut(s) 93
PfeI GAWTC 1 cut(s) 293
PflMI CCANNNNNTGG 1 cut(s) 457
PkrI GCNGC 1 cut(s) 163
Ple19I CGATCG 1 cut(s) 37
PscI ACATGT 1 cut(s) 93
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 499
PspGI CCWGG 1 cut(s) 499
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 1 cut(s) 497
PstI CTGCAG 1 cut(s) 355
PvuI CGATCG 1 cut(s) 37
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 1 cut(s) 256
RsaNI GTAC 1 cut(s) 255
RseI CAYNNNNRTG 2 cut(s) 92, 333
SacI GAGCTC 1 cut(s) 234
SatI GCNGC 1 cut(s) 162
Sau3AI GATC 3 cut(s) 34, 40, 337
Sau96I GGNCC 1 cut(s) 497
ScrFI CCNGG 1 cut(s) 501
SduI GDGCHC 1 cut(s) 234
SetI ASST 7 cut(s) 129, 135, 234, 241, 310, 352, 401
SfcI CTRYAG 1 cut(s) 351
SmiMI CAYNNNNRTG 2 cut(s) 92, 333
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 4 cut(s) 23, 71, 384, 435
SspMI CTAG 1 cut(s) 213
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 499
TaaI ACNGT 2 cut(s) 335, 428
TaqI TCGA 1 cut(s) 37
TasI AATT 4 cut(s) 23, 71, 384, 435
TatI WGTACW 1 cut(s) 254
TfiI GAWTC 1 cut(s) 293
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 1 cut(s) 161
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 1 cut(s) 185
TspGWI ACGGA 1 cut(s) 236
Van91I CCANNNNNTGG 1 cut(s) 457
XapI RAATTY 2 cut(s) 23, 435
XceI RCATGY 1 cut(s) 97
XspI CTAG 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.