Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0008542

Physical Location & Seq

Genomic Coordinates Reverse (-)

13976667 .. 13980443

Gene Structure 3777 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1168900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1434 bp

ATGGGGTTTGGAAAGTTCCAAATTCTTGTGCTTGCATATGCCGGCATGGGTTGGGTCTCAGAAGCCATGGAGATGATGCTACTCTCTTTTGTTGGACCAGCGGTTCAGTCTGCTTGGGGTCTTTCGTCTGAACAAGAAAGTTTAATAACCAGTGTGGTTTTTGTTGGAACGCTAGTCGGAGCTTACTCGTGGGGCATAGTTTCTGACAAATATGGACGTAGGAAAGGATTCCTCATTACAGCCATAGTTACTTCTGGAGCTGGTTTTCTGAGTGCTCTTTCCCCCAATTATACATCCTTGCTCGTCCTTCGTTGCATAGTTGGTGTTGGTTTGGGAGGTGGCCCTGTACTCTCATCCTGGTTTCTGGAGTTCATTCCTGCTCCTAACAGAGGTACCTGGATGGTTATTTTTTCAGCTTTTTGGACTGTTGGAACAATTCTTGAGGCTTCACTTGCGTGGCTTGTCATGCCAAACTTTGGTTGGAGGTGGCTACTTGCCTTGTCTTCACTCCCTTCATCGCTTCTCCTCATATTCTATTGGGTTGCACCTGAATCACCGAGATATTTGTGCTTAAAAGGTAGAACAACTGAGGCAATCAATATTTTGGAGAAAGTAGCAAGAATGAATGGAACGAAACTCCCTTCGGGGAATCTTGTTTCTGATTTGAAAATTGAGTTACCTGAAAATAGTACTCCATCAGAAGCTACGCGTTTGATCTCACCGACAGATAAAGAAGATCCAACTCCTAAGGAGATGGATTCTAATGTGGGGGGCATCTCTTCACTGTTAACGCTTCTTTCCCCAAAATTAATCAGATCAACCCTGCTCTTATGGGTAGTATTCTTTGGGAATGCTTTTTCATATTACGGCCTGGTGCTTCTGACAACTGAGTTGAGCAGCAGACACAGTACATGTACACCGGGTCCAATACAGTCGGATAAAGCGATGGATACTAGCTATAGAGATGTTTTCATCACTAGTATTGCAGAGTTCCCTGGGCTCCTCATATCTGCTGCTACGGTCGATAAACTGGGTCGTAAGCTTTCAATGTCATCCATGTTCTTCCTATGCTGCATTTTTCTACTCCCCTTGGTAGTCCATCAGTCTCAGGGCTTGACCACTATCCTTCTGTTTGGAGCTCGCATATGCATCACAGGGTCCTTCACGATAATCTACATATATGCCCCAGAGATATACCCAACCTCAGTCCGAACAACTGGTGTTGGAATCGCAAGTGCAATGGGAAGAATAGGTGGAATGTTATGCCCTCTTGTGGCAGTAGGTTTGGTGCATGGATGCCATCAAACAGCTTCCATTCTGCTGTTTGAGATTATAATTTTTCTTTCGGGGATATGCGTGTTGCTCTTCCCGTTTGAAACCAAGGGCCTTGAATTGAGCGATACTATATCGGATTCAAAGACTCAAAGGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006820 GO:0006855 GO:0008028 GO:0008150 GO:0008324 GO:0008509 GO:0008514 GO:0008519 GO:0015075 GO:0015101 GO:0015238 GO:0015318 GO:0015651 GO:0015695 GO:0015696 GO:0015697 GO:0015711 GO:0015718 GO:0015849 GO:0015893 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0034220 GO:0035461 GO:0042221 GO:0042493 GO:0044425 GO:0044464 GO:0046942 GO:0046943 GO:0050896 GO:0051179 GO:0051180 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:0072488 GO:0090416 GO:0090417 GO:0090482 GO:0098655 GO:0098656 GO:1903825 GO:1905039 GO:2001142 GO:2001143

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFS_1 Sugar_tr

Protein Analysis

478

Amino Acids

51.71

Weight (kDa)

7.51

Isoelectric Point (pI)

37.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	20 - 295	1.3e-26	Major Facilitator Superfamily
Sugar_tr	PF00083	42 - 466	7.8e-44	Sugar (and other) transporter
MFS_1	PF07690	325 - 460	1.4e-11	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13050 AT3G13050 AT3G13050 AT3G13050
fragaria_vesca	FvH4_6g36460 FvH4_6g36460 FvH4_6g36460
malus_domestica	MD09G1168900.v1.1 MD17G1157900.v1.1
prunus_persica	Prupe.3G027200_v2.0.a1 Prupe.3G027200_v2.0.a1
pyrus_communis	pycom09g08650
rosa_chinensis	RchiOBHm_Chr2g0148591
rosa_laevigata	RLG00000020353
rosa_roxburghii	Rroxscaffold_2G00098610
rosa_rugosa	Rorug02G0407400 Rorug02G0407500 Rorug02G0407600
rosa_samantha	Rh2AG466300 Rh2BG479400 Rh2CG452800 Rh2DG488200
rosa_wichuraiana	Rw2G038020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1334
Acc65I	GGTACC	1 cut(s)	392
AccB1I	GGYRCC	1 cut(s)	392
AccB7I	CCANNNNNTGG	1 cut(s)	476
AccII	CGCG	1 cut(s)	709
AciI	CCGC	1 cut(s)	101
AclWI	GGATC	1 cut(s)	731
AcsI	RAATTY	1 cut(s)	21
AfaI	GTAC	5 cut(s)	348, 394, 691, 910, 916
AfiI	CCNNNNNNNGG	3 cut(s)	389, 476, 1273
AflIII	ACRYGT	2 cut(s)	707, 911
AgsI	TTSAA	5 cut(s)	667, 1047, 1376, 1391, 1416
AhlI	ACTAGT	1 cut(s)	977
AjnI	CCWGG	4 cut(s)	356, 395, 870, 994
AleI	CACNNNNGTG	1 cut(s)	454
AloI	GAACNNNNNNTCC	2 cut(s)	87, 119
AluBI	AGCT	8 cut(s)	182, 260, 416, 704, 957, 1042, 1139, 1310
AluI	AGCT	8 cut(s)	182, 260, 416, 704, 957, 1042, 1139, 1310
Alw21I	GWGCWC	2 cut(s)	277, 1141
Alw26I	GTCTC	2 cut(s)	61, 1110
AlwI	GGATC	1 cut(s)	731
AoxI	GGCC	3 cut(s)	340, 868, 1384
ApeKI	GCWGC	3 cut(s)	897, 1013, 1071
ApoI	RAATTY	1 cut(s)	21
AseI	ATTAAT	1 cut(s)	809
Asp700I	GAANNNNTTC	1 cut(s)	227
Asp718I	GGTACC	1 cut(s)	392
AspS9I	GGNCC	5 cut(s)	95, 341, 923, 1158, 1384
AsuC2I	CCSGG	1 cut(s)	921
AsuHPI	GGTGA	2 cut(s)	546, 711
AvaII	GGWCC	3 cut(s)	95, 923, 1158
AxyI	CCTNAGG	1 cut(s)	747
BaeI	ACNNNNGTAYC	2 cut(s)	376, 409
BanI	GGYRCC	1 cut(s)	392
BanII	GRGCYC	2 cut(s)	1002, 1141
BauI	CACGAG	1 cut(s)	187
BbsI	GAAGAC	1 cut(s)	495
Bbv12I	GWGCWC	2 cut(s)	277, 1141
BbvI	GCAGC	3 cut(s)	909, 1000, 1058
BccI	CCATC	6 cut(s)	394, 703, 748, 940, 1107, 1308
BceAI	ACGGC	1 cut(s)	883
BciT130I	CCWGG	4 cut(s)	358, 397, 872, 996
BciVI	GTATCC	1 cut(s)	943
BcnI	CCSGG	1 cut(s)	921
BcoDI	GTCTC	2 cut(s)	61, 1110
BcuI	ACTAGT	1 cut(s)	977
BfaI	CTAG	3 cut(s)	173, 954, 978
BfmI	CTRYAG	1 cut(s)	958
BfuI	GTATCC	1 cut(s)	943
BisI	GCNGC	3 cut(s)	898, 1014, 1072
BlsI	GCNGC	3 cut(s)	899, 1015, 1073
BmcAI	AGTACT	1 cut(s)	691
Bme1390I	CCNGG	5 cut(s)	358, 397, 872, 921, 996
Bme18I	GGWCC	3 cut(s)	95, 923, 1158
BmgT120I	GGNCC	5 cut(s)	95, 341, 923, 1158, 1384
BmiI	GGNNCC	4 cut(s)	394, 924, 1001, 1159
BmrFI	CCNGG	5 cut(s)	358, 397, 872, 921, 996
BmrI	ACTGGG	1 cut(s)	1040
BmsI	GCATC	4 cut(s)	66, 783, 1158, 1286
BmuI	ACTGGG	1 cut(s)	1040
BpiI	GAAGAC	1 cut(s)	495
BpmI	CTGGAG	2 cut(s)	276, 386
BpuEI	CTTGAG	1 cut(s)	461
BpuMI	CCSGG	1 cut(s)	921
BsaI	GGTCTC	1 cut(s)	61
BsaJI	CCNNGG	5 cut(s)	66, 994, 995, 1089, 1380
Bsc4I	CCNNNNNNNGG	3 cut(s)	389, 476, 1273
Bse118I	RCCGGY	1 cut(s)	41
Bse1I	ACTGG	3 cut(s)	150, 1035, 1222
Bse21I	CCTNAGG	1 cut(s)	747
Bse3DI	GCAATG	1 cut(s)	1245
BseBI	CCWGG	4 cut(s)	358, 397, 872, 996
BseDI	CCNNGG	5 cut(s)	66, 994, 995, 1089, 1380
BseGI	GGATG	5 cut(s)	293, 353, 405, 1052, 1301
BseLI	CCNNNNNNNGG	3 cut(s)	389, 476, 1273
BseMI	GCAATG	1 cut(s)	1245
BseMII	CTCAG	6 cut(s)	72, 260, 579, 879, 1121, 1218
BseNI	ACTGG	3 cut(s)	150, 1035, 1222
BseRI	GAGGAG	2 cut(s)	515, 992
BseXI	GCAGC	3 cut(s)	909, 1000, 1058
Bsh1236I	CGCG	1 cut(s)	709
Bsh1285I	CGRYCG	1 cut(s)	1023
BshFI	GGCC	3 cut(s)	342, 870, 1386
BshNI	GGYRCC	1 cut(s)	392
BsiEI	CGRYCG	1 cut(s)	1023
BsiHKAI	GWGCWC	2 cut(s)	277, 1141
BsiSI	CCGG	2 cut(s)	42, 920
BslI	CCNNNNNNNGG	3 cut(s)	389, 476, 1273
BsmAI	GTCTC	2 cut(s)	61, 1110
BsmI	GAATGC	1 cut(s)	856
BsnI	GGCC	3 cut(s)	342, 870, 1386
Bso31I	GGTCTC	1 cut(s)	61
Bsp1286I	GDGCHC	3 cut(s)	277, 1002, 1141
Bsp1407I	TGTACA	1 cut(s)	914
Bsp143I	GATC	3 cut(s)	714, 736, 815
Bsp19I	CCATGG	1 cut(s)	66
BspACI	CCGC	1 cut(s)	101
BspANI	GGCC	3 cut(s)	342, 870, 1386
BspCNI	CTCAG	6 cut(s)	71, 261, 580, 880, 1120, 1217
BspFNI	CGCG	1 cut(s)	709
BspLI	GGNNCC	4 cut(s)	394, 924, 1001, 1159
BspPI	GGATC	1 cut(s)	731
BspQI	GCTCTTC	1 cut(s)	1370
BspT107I	GGYRCC	1 cut(s)	392
BspTNI	GGTCTC	1 cut(s)	61
BsrDI	GCAATG	1 cut(s)	1245
BsrFI	RCCGGY	1 cut(s)	41
BsrGI	TGTACA	1 cut(s)	914
BsrI	ACTGG	3 cut(s)	150, 1035, 1222
BssAI	RCCGGY	1 cut(s)	41
BssECI	CCNNGG	5 cut(s)	66, 994, 995, 1089, 1380
BssMI	GATC	3 cut(s)	714, 736, 815
BssSI	CACGAG	1 cut(s)	187
BssT1I	CCWWGG	3 cut(s)	66, 1089, 1380
Bst2BI	CACGAG	1 cut(s)	187
Bst2UI	CCWGG	4 cut(s)	358, 397, 872, 996
Bst4CI	ACNGT	5 cut(s)	427, 786, 908, 933, 1021
Bst6I	CTCTTC	2 cut(s)	784, 1370
BstAUI	TGTACA	1 cut(s)	914
BstC8I	GCNNGC	3 cut(s)	33, 43, 1141
BstDEI	CTNAG	7 cut(s)	58, 269, 588, 747, 888, 1107, 1204
BstDSI	CCRYGG	1 cut(s)	66
BstENI	CCTNNNNNAGG	1 cut(s)	387
BstF5I	GGATG	5 cut(s)	293, 353, 405, 1052, 1301
BstFNI	CGCG	1 cut(s)	709
BstKTI	GATC	3 cut(s)	717, 739, 818
BstMAI	GTCTC	2 cut(s)	61, 1110
BstMBI	GATC	3 cut(s)	714, 736, 815
BstMCI	CGRYCG	1 cut(s)	1023
BstMWI	GCNNNNNNNGC	2 cut(s)	452, 466
BstNI	CCWGG	4 cut(s)	358, 397, 872, 996
BstNSI	RCATGY	1 cut(s)	915
BstSCI	CCNGG	5 cut(s)	356, 395, 870, 919, 994
BstSFI	CTRYAG	1 cut(s)	958
BstUI	CGCG	1 cut(s)	709
BstV1I	GCAGC	3 cut(s)	909, 1000, 1058
BstV2I	GAAGAC	1 cut(s)	495
BstX2I	RGATCY	1 cut(s)	736
BstYI	RGATCY	1 cut(s)	736
Bsu36I	CCTNAGG	1 cut(s)	747
BsuI	GTATCC	1 cut(s)	943
BsuRI	GGCC	3 cut(s)	342, 870, 1386
BtgI	CCRYGG	1 cut(s)	66
BtgZI	GCGATG	2 cut(s)	501, 959
BtsCI	GGATG	5 cut(s)	293, 353, 405, 1052, 1301
BtsIMutI	CAGTG	2 cut(s)	157, 782
Cac8I	GCNNGC	3 cut(s)	33, 43, 1141
Cfr10I	RCCGGY	1 cut(s)	41
Cfr13I	GGNCC	5 cut(s)	95, 341, 923, 1158, 1384
Csp6I	GTAC	5 cut(s)	347, 393, 690, 909, 915
CviAII	CATG	6 cut(s)	46, 67, 466, 912, 1057, 1292
CviQI	GTAC	5 cut(s)	347, 393, 690, 909, 915
DdeI	CTNAG	7 cut(s)	58, 269, 588, 747, 888, 1107, 1204
DpnI	GATC	3 cut(s)	716, 738, 817
DpnII	GATC	3 cut(s)	714, 736, 815
Eam1104I	CTCTTC	2 cut(s)	784, 1370
EarI	CTCTTC	2 cut(s)	784, 1370
Ecl136II	GAGCTC	1 cut(s)	1139
Eco130I	CCWWGG	3 cut(s)	66, 1089, 1380
Eco24I	GRGCYC	2 cut(s)	1002, 1141
Eco31I	GGTCTC	1 cut(s)	61
Eco47I	GGWCC	3 cut(s)	95, 923, 1158
Eco53kI	GAGCTC	1 cut(s)	1139
Eco81I	CCTNAGG	1 cut(s)	747
EcoICRI	GAGCTC	1 cut(s)	1139
EcoNI	CCTNNNNNAGG	1 cut(s)	387
EcoO109I	RGGNCCY	2 cut(s)	1158, 1384
EcoRII	CCWGG	4 cut(s)	356, 395, 870, 994
EcoT14I	CCWWGG	3 cut(s)	66, 1089, 1380
EcoT22I	ATGCAT	1 cut(s)	1151
EcoT38I	GRGCYC	2 cut(s)	1002, 1141
ErhI	CCWWGG	3 cut(s)	66, 1089, 1380
FaeI	CATG	6 cut(s)	49, 70, 469, 915, 1060, 1295
FatI	CATG	6 cut(s)	45, 66, 465, 911, 1056, 1291
FauNDI	CATATG	2 cut(s)	37, 1145
Fnu4HI	GCNGC	3 cut(s)	898, 1014, 1072
FokI	GGATG	5 cut(s)	280, 340, 412, 1039, 1308
FriOI	GRGCYC	2 cut(s)	1002, 1141
Fsp4HI	GCNGC	3 cut(s)	898, 1014, 1072
FspBI	CTAG	3 cut(s)	173, 954, 978
GluI	GCNGC	3 cut(s)	898, 1014, 1072
GsuI	CTGGAG	2 cut(s)	276, 386
HaeIII	GGCC	3 cut(s)	342, 870, 1386
HapII	CCGG	2 cut(s)	42, 920
Hin1II	CATG	6 cut(s)	49, 70, 469, 915, 1060, 1295
HincII	GTYRAC	1 cut(s)	789
HindII	GTYRAC	1 cut(s)	789
HindIII	AAGCTT	1 cut(s)	1040
HinfI	GANTC	7 cut(s)	228, 551, 649, 758, 1227, 1412, 1420
HpaI	GTTAAC	1 cut(s)	789
HpaII	CCGG	2 cut(s)	42, 920
HphI	GGTGA	2 cut(s)	546, 711
Hpy166II	GTNNAC	2 cut(s)	789, 917
Hpy188III	TCNNGA	4 cut(s)	255, 365, 440, 1165
Hpy8I	GTNNAC	2 cut(s)	789, 917
HpyAV	CCTTC	5 cut(s)	317, 522, 651, 1136, 1171
HpyCH4III	ACNGT	5 cut(s)	427, 786, 908, 933, 1021
HpyCH4IV	ACGT	1 cut(s)	217
HpyCH4V	TGCA	8 cut(s)	35, 315, 545, 986, 1074, 1149, 1238, 1291
HpyF10VI	GCNNNNNNNGC	2 cut(s)	452, 466
HpyF3I	CTNAG	7 cut(s)	58, 269, 588, 747, 888, 1107, 1204
HpySE526I	ACGT	1 cut(s)	217
Hsp92II	CATG	6 cut(s)	49, 70, 469, 915, 1060, 1295
KpnI	GGTACC	1 cut(s)	396
KroI	GCCGGC	1 cut(s)	41
KroNI	GCCGGC	1 cut(s)	43
KspAI	GTTAAC	1 cut(s)	789
Kzo9I	GATC	3 cut(s)	714, 736, 815
LguI	GCTCTTC	1 cut(s)	1370
LmnI	GCTCC	5 cut(s)	179, 257, 385, 1005, 1136
Lsp1109I	GCAGC	3 cut(s)	909, 1000, 1058
LweI	GCATC	4 cut(s)	66, 783, 1158, 1286
MaeI	CTAG	3 cut(s)	173, 954, 978
MaeII	ACGT	1 cut(s)	217
MaeIII	GTNAC	2 cut(s)	247, 675
MalI	GATC	3 cut(s)	716, 738, 817
MboI	GATC	3 cut(s)	714, 736, 815
MboII	GAAGA	6 cut(s)	495, 746, 771, 1054, 1257, 1357
MflI	RGATCY	1 cut(s)	736
MhlI	GDGCHC	3 cut(s)	277, 1002, 1141
MluCI	AATT	7 cut(s)	21, 286, 435, 669, 806, 1335, 1391
MluI	ACGCGT	1 cut(s)	707
MlyI	GAGTC	1 cut(s)	1414
MmeI	TCCRAC	8 cut(s)	73, 145, 157, 409, 461, 764, 915, 1204
Mph1103I	ATGCAT	1 cut(s)	1151
MroNI	GCCGGC	1 cut(s)	41
MroXI	GAANNNNTTC	1 cut(s)	227
MseI	TTAA	4 cut(s)	143, 572, 788, 809
MslI	CAYNNNNRTG	2 cut(s)	71, 454
MspA1I	CMGCKG	1 cut(s)	101
MspI	CCGG	2 cut(s)	42, 920
MspR9I	CCNGG	5 cut(s)	358, 397, 872, 921, 996
Mva1269I	GAATGC	1 cut(s)	856
MvaI	CCWGG	4 cut(s)	358, 397, 872, 996
MvnI	CGCG	1 cut(s)	709
MwoI	GCNNNNNNNGC	2 cut(s)	452, 466
NaeI	GCCGGC	1 cut(s)	43
NciI	CCSGG	1 cut(s)	921
NcoI	CCATGG	1 cut(s)	66
NdeI	CATATG	2 cut(s)	37, 1145
NdeII	GATC	3 cut(s)	714, 736, 815
NgoMIV	GCCGGC	1 cut(s)	41
NlaIII	CATG	6 cut(s)	49, 70, 469, 915, 1060, 1295
NlaIV	GGNNCC	4 cut(s)	394, 924, 1001, 1159
NsiI	ATGCAT	1 cut(s)	1151
NspI	RCATGY	1 cut(s)	915
OliI	CACNNNNGTG	1 cut(s)	454
PasI	CCCWGGG	1 cut(s)	995
PciI	ACATGT	1 cut(s)	911
PciSI	GCTCTTC	1 cut(s)	1370
PcsI	WCGNNNNNNNCGW	1 cut(s)	941
PctI	GAATGC	1 cut(s)	856
PdiI	GCCGGC	1 cut(s)	43
PdmI	GAANNNNTTC	1 cut(s)	227
PfeI	GAWTC	6 cut(s)	228, 551, 649, 758, 1227, 1412
PflMI	CCANNNNNTGG	1 cut(s)	476
PkrI	GCNGC	3 cut(s)	899, 1015, 1073
PleI	GAGTC	1 cut(s)	1414
PpsI	GAGTC	1 cut(s)	1414
PpuMI	RGGWCCY	1 cut(s)	1158
PscI	ACATGT	1 cut(s)	911
PshBI	ATTAAT	1 cut(s)	809
PsiI	TTATAA	1 cut(s)	1334
Psp124BI	GAGCTC	1 cut(s)	1141
Psp5II	RGGWCCY	1 cut(s)	1158
Psp6I	CCWGG	4 cut(s)	356, 395, 870, 994
PspGI	CCWGG	4 cut(s)	356, 395, 870, 994
PspN4I	GGNNCC	4 cut(s)	394, 924, 1001, 1159
PspPI	GGNCC	5 cut(s)	95, 341, 923, 1158, 1384
PspPPI	RGGWCCY	1 cut(s)	1158
PsuI	RGATCY	1 cut(s)	736
RsaI	GTAC	5 cut(s)	348, 394, 691, 910, 916
RsaNI	GTAC	5 cut(s)	347, 393, 690, 909, 915
RseI	CAYNNNNRTG	2 cut(s)	71, 454
SacI	GAGCTC	1 cut(s)	1141
SapI	GCTCTTC	1 cut(s)	1370
SaqAI	TTAA	4 cut(s)	143, 572, 788, 809
SatI	GCNGC	3 cut(s)	898, 1014, 1072
Sau3AI	GATC	3 cut(s)	714, 736, 815
Sau96I	GGNCC	5 cut(s)	95, 341, 923, 1158, 1384
ScaI	AGTACT	1 cut(s)	691
SchI	GAGTC	1 cut(s)	1414
ScrFI	CCNGG	5 cut(s)	358, 397, 872, 921, 996
SduI	GDGCHC	3 cut(s)	277, 1002, 1141
SfaNI	GCATC	4 cut(s)	66, 783, 1158, 1286
SfcI	CTRYAG	1 cut(s)	958
SinI	GGWCC	3 cut(s)	95, 923, 1158
SmiMI	CAYNNNNRTG	2 cut(s)	71, 454
SmlI	CTYRAG	1 cut(s)	440
SmoI	CTYRAG	1 cut(s)	440
SpeI	ACTAGT	1 cut(s)	977
Sse9I	AATT	7 cut(s)	21, 286, 435, 669, 806, 1335, 1391
SsiI	CCGC	1 cut(s)	101
SspI	AATATT	1 cut(s)	601
SspMI	CTAG	3 cut(s)	173, 954, 978
SstI	GAGCTC	1 cut(s)	1141
StyD4I	CCNGG	5 cut(s)	356, 395, 870, 919, 994
StyI	CCWWGG	3 cut(s)	66, 1089, 1380
TaaI	ACNGT	5 cut(s)	427, 786, 908, 933, 1021
TaiI	ACGT	1 cut(s)	220
TaqI	TCGA	1 cut(s)	1023
TasI	AATT	7 cut(s)	21, 286, 435, 669, 806, 1335, 1391
TatI	WGTACW	4 cut(s)	346, 689, 908, 914
TfiI	GAWTC	6 cut(s)	228, 551, 649, 758, 1227, 1412
Tru1I	TTAA	4 cut(s)	143, 572, 788, 809
Tru9I	TTAA	4 cut(s)	143, 572, 788, 809
TscAI	CASTG	2 cut(s)	157, 789
TseI	GCWGC	3 cut(s)	897, 1013, 1071
TspDTI	ATGAA	5 cut(s)	361, 504, 638, 849, 961
TspRI	CASTG	2 cut(s)	157, 789
Van91I	CCANNNNNTGG	1 cut(s)	476
VpaK11BI	GGWCC	3 cut(s)	95, 923, 1158
VspI	ATTAAT	1 cut(s)	809
XagI	CCTNNNNNAGG	1 cut(s)	387
XapI	RAATTY	1 cut(s)	21
XceI	RCATGY	1 cut(s)	915
XcmI	CCANNNNNNNNNTGG	1 cut(s)	477
XmnI	GAANNNNTTC	1 cut(s)	227
XspI	CTAG	3 cut(s)	173, 954, 978
ZrmI	AGTACT	1 cut(s)	691
Zsp2I	ATGCAT	1 cut(s)	1151

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.