Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0013037

Physical Location & Seq

Genomic Coordinates Forward (+)

15483422 .. 15485797

Gene Structure 2376 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1180500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2319 bp

ATGGCCGGTTCCAACCTCCTTCCCTTCCTTTTCCTCCTCTGTTTTCTTGTTCCTGCTGCGCTCTCGATCCCGCCTGACCTATCCGACCGTCCAAAAACCTTCATCGTCCACGTGTCCAAATCAGACAAGCCCGCCCTCTTCTCCTCTCACCGGAGCTGGTACACCTCCATCATCCAATCCCTCCCGTCCCCCCACCCCACCAAACTCCTCTACACATACGACCGCGCCGTCCACGGCTTCTCCGCCGCCCTCACATCCTCTCAGGCCACCCAGCTCCTCTCCCACCCCGCCGTCCTCTCCGTCACGCCCGACCAGCCACGTCAGCTCCACACCACTCACACCCCCAGCTTCCTCGGCCTCGCTGAATCCTTCGGCTTATGGCCCAACTCCGACTACGCCGACGACGTCATAGTCGGCGTCCTCGACACCGGGATCTGGCCGGAGCGCCCGAGCTTCTCGGACTCCGGTCTCGGCCCGGTTCCGTCCCGATGGAAGGGCACCTGCGTCAGTGCCCCGGACTTCCCGTCCTCCTCCTGCAACCGCAAGATCATCGGCGCCAGAGCCTACTTCGACGGCTACGAAGCCTACATCGGACGATTAATGGACGAATCGAATGAGTCGAAATCGCCCAGAGACACCGAAGGCCATGGGACACACACGGCCTCCACCGCCGCCGGCTCCCCGGTCGCTAACGCCAGCTTGTTCTCGTACGCTCGCGGCGAAGCCAGAGGCATGGCCACCAAGGCGAGAATCGCCGTCTATAAAATCTGCTGGTCATTTGGGTGTTTTGATTCCGACATTTTGGCCGCTATGGATCAAGCAATTGCCGACGGCGTTGACATAATTTCGCTCTCCGTCGGAGCCAGCGGTCGTTCTCCAGCGTACGATCGAGACTCCATAGCCATCGGATCCTTCGGGGCTGCCCAGCATGGTGTTCTCGTCTCCGCCTCCGCCGGTAATTCTGGGCCCAACCCATTTACGGCCACCAACATAGCTCCATGGATTCTCACAGTCGGCGCTTCCACCGTTGACAGAGAGTTCCCAGCCGATGTTATCCTCGGCGATAACCGGGTTTTCGGAGGTGTTTCATTGTACTCCGGTGAGCCTCTAGTCGATCACCAGCTTCCATTAATATACGGCGGAGACGCCGGAAGCAGGTACTGTTACGCAGGGGCTCTCAAGCCATCGAAAGTCCAGGGAAAAATTGTCGTCTGTGATCGCGGCGGCAATGCGAGAGTCGGAAAGGGAAGCGCGGTGAAGCTAGCCGGCGGGCTCGGGTTTATCTTGGCCAACACAGCCGACAGCGGAGAAGAGCTTCTCGCTGATGCACACCTTATCCCAGCAACAGAGGTGGGTGAAATCGCCGCCGATCAGATAAGGGAATACATCAGATTGAGCCAATACCCGACGGCCACAATTGTTTTCCGAGGAACTGTAGTTGGGACTTCGCCGGCGGCCCCAAAGGTCGCAGCTTTTTCGAGCAGAGGACCGAATTCTCTAACACCAGAGATTCTCAAGCCCGATGTGATTGCTCCTGGAGTGAACATTTTGGCGGGTTGGACTGGGGCGTCTGCCCCCACAGATTTGGACATTGATCCTAGAAGAACCGAGTTCAATATAATTTCGGGGACATCTATGTCGTGCCCGCATGTGAGCGGCATTGCTGCATTGCTTCGAAAGGCCTATCCGAATTGGTCGATTGCTGCTATAAAATCTGCTTTGATCACAACAGCTTACACTTTAGACGATTCAGGTAAGAAAATCAGGGATCTTGCAACAGGGAAAGAGTCGACTCCTTTTGTTCATGGAGCAGGACATGTTGATCCCAACAGAGCTCTCAATCCCGGGTTGATTTACGATCTCAATGTGAATGACTATGTTGCATTTTTGTGCTCAATCGGGTACAGTCCAAGGCAAATCGCTGTTTTCTTAGGAAAGCCTACTGGTTCTGATATATGCACAAAGAACAGTTTGGCTAGTCCTGGTGATCTGAATTACCCTTCACTTTCCGTCATCTTGAGCTCGGATCAAAGCCTGGTTAAGTACAAGAGAGTCGCTACGAATGTGGGGGGCAATGTGGATGCGGTTTATAAGGTGAATGTTACTGCTCCGGCTGGTGTTGAGATCAGCGTTTCCCCGAGCAAGCTGGAGTTCAGTGAAGAAAACCAGAGTCTGAGCTATGAGGTTACATTCAGGAGAGGAGTTGGGTATGATGGCGGAGAGCGATACGGGTCTATTGAGTGGACAGACGGTAGCCATCTGGTGAGGAGTCCGGTTGCTGTGAGGTGGAGCAGCACTCGGTATACAGCTTCCATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001763 GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006508 GO:0006807 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009505 GO:0009653 GO:0010016 GO:0010150 GO:0010223 GO:0010346 GO:0016020 GO:0016787 GO:0017171 GO:0019538 GO:0030312 GO:0031090 GO:0032501 GO:0032502 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0048366 GO:0048367 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0070011 GO:0071704 GO:0071944 GO:0090693 GO:0098588 GO:0098805 GO:0099402 GO:0140096 GO:1901564 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

773

Amino Acids

81.67

Weight (kDa)

6.26

Isoelectric Point (pI)

36.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	33 - 110	1.4e-18	Peptidase inhibitor I9
Peptidase_S8	PF00082	134 - 589	7.3e-51	Subtilase family
PA	PF02225	375 - 458	8e-11	PA domain
fn3_6	PF17766	663 - 761	9.8e-27	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013037)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14067
fragaria_vesca	FvH4_6g35010
malus_domestica	MD09G1180500.v1.1 MD17G1166500.v1.1
prunus_persica	Prupe.3G039000_v2.0.a1
pyrus_communis	pycom09g09790
rosa_chinensis	RchiOBHm_Chr2g0146061
rosa_laevigata	RLG00000020182
rosa_multiflora	Rmu_sc0010454.1_g000002 Rmu_sc0014682.1_g000001
rosa_roxburghii	Rroxscaffold_152G00434860 Rroxscaffold_2G00100300
rosa_rugosa	Rorug02G0393600
rosa_samantha	Rh2AG449000 Rh2BG461400 Rh2CG436000 Rh2DG470600
rosa_wichuraiana	Rw2G036670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2091
AarI	CACCTGC	1 cut(s)	509
AasI	GACNNNNNNGTC	4 cut(s)	227, 410, 1567, 1636
AatII	GACGTC	1 cut(s)	408
Acc36I	ACCTGC	2 cut(s)	509, 1146
AccB1I	GGYRCC	2 cut(s)	497, 554
AccBSI	CCGCTC	1 cut(s)	1656
AccI	GTMKAC	2 cut(s)	1790, 2303
AccII	CGCG	4 cut(s)	225, 717, 1221, 1253
AclWI	GGATC	9 cut(s)	61, 440, 822, 903, 916, 1589, 1776, 1817, 2034
AcoI	YGGCCR	7 cut(s)	3, 437, 735, 804, 981, 1287, 1410
AcsI	RAATTY	1 cut(s)	1492
AcvI	CACGTG	1 cut(s)	112
AcyI	GRCGYC	5 cut(s)	405, 417, 555, 1146, 1568
AfaI	GTAC	7 cut(s)	161, 710, 884, 1094, 1160, 1904, 2045
AfiI	CCNNNNNNNGG	5 cut(s)	150, 435, 493, 979, 1155
AflIII	ACRYGT	2 cut(s)	111, 1816
AgsI	TTSAA	1 cut(s)	1615
AhdI	GACNNNNNGTC	1 cut(s)	523
AjiI	CACGTC	1 cut(s)	320
AjnI	CCWGG	4 cut(s)	1194, 1534, 1981, 2034
Alw21I	GWGCWC	3 cut(s)	1837, 1895, 2024
Alw26I	GTCTC	5 cut(s)	473, 627, 885, 946, 1137
AlwI	GGATC	9 cut(s)	61, 440, 822, 903, 916, 1589, 1776, 1817, 2034
AlwNI	CAGNNNCTG	2 cut(s)	1161, 2173
Ama87I	CYCGRG	4 cut(s)	448, 1274, 1844, 2137
ApaI	GGGCCC	1 cut(s)	969
ApeKI	GCWGC	6 cut(s)	56, 920, 1469, 1664, 1703, 2292
ApoI	RAATTY	1 cut(s)	1492
AseI	ATTAAT	2 cut(s)	599, 1130
Asp700I	GAANNNNTTC	1 cut(s)	1314
AspLEI	GCGC	6 cut(s)	61, 227, 447, 557, 1019, 1253
AspS9I	GGNCC	6 cut(s)	381, 473, 965, 966, 1456, 1487
AsuC2I	CCSGG	7 cut(s)	430, 476, 515, 683, 1070, 1845, 1846
AsuHPI	GGTGA	8 cut(s)	140, 1109, 1112, 1267, 1367, 1997, 2107, 2275
AsuII	TTCGAA	1 cut(s)	1675
AsuNHI	GCTAGC	1 cut(s)	1261
AvaI	CYCGRG	4 cut(s)	448, 1274, 1844, 2137
AvaII	GGWCC	1 cut(s)	1487
BaeGI	GKGCMC	4 cut(s)	500, 514, 969, 1646
BalI	TGGCCA	2 cut(s)	737, 1289
BamHI	GGATCC	1 cut(s)	908
BanI	GGYRCC	2 cut(s)	497, 554
BanII	GRGCYC	5 cut(s)	969, 1177, 1275, 1837, 2024
BbrPI	CACGTG	1 cut(s)	112
Bbv12I	GWGCWC	3 cut(s)	1837, 1895, 2024
BbvI	GCAGC	6 cut(s)	43, 907, 1481, 1651, 1690, 2304
BccI	CCATC	6 cut(s)	176, 483, 911, 1192, 2207, 2265
BcgI	CGANNNNNNTGC	6 cut(s)	532, 566, 1458, 1492, 1511, 1545
BciT130I	CCWGG	4 cut(s)	1196, 1536, 1983, 2036
BclI	TGATCA	1 cut(s)	1722
BcnI	CCSGG	7 cut(s)	430, 476, 515, 683, 1070, 1845, 1846
BcoDI	GTCTC	5 cut(s)	473, 627, 885, 946, 1137
BfaI	CTAG	4 cut(s)	1109, 1262, 1599, 1977
BfmI	CTRYAG	1 cut(s)	1434
BfoI	RGCGCY	3 cut(s)	448, 558, 1020
BfuAI	ACCTGC	2 cut(s)	509, 1146
BglI	GCCNNNNNGGC	1 cut(s)	743
Bme18I	GGWCC	1 cut(s)	1487
BmeRI	GACNNNNNGTC	1 cut(s)	523
BmeT110I	CYCGRG	4 cut(s)	448, 1274, 1844, 2137
BmgBI	CACGTC	1 cut(s)	320
BmgT120I	GGNCC	6 cut(s)	381, 473, 965, 966, 1456, 1487
BmiI	GGNNCC	9 cut(s)	10, 480, 499, 556, 679, 862, 910, 967, 1458
BmrI	ACTGGG	1 cut(s)	1572
BmsI	GCATC	2 cut(s)	1315, 2071
BmtI	GCTAGC	1 cut(s)	1265
BmuI	ACTGGG	1 cut(s)	1572
BoxI	GACNNNNGTC	1 cut(s)	465
BplI	GAGNNNNNCTC	2 cut(s)	2281, 2313
BpmI	CTGGAG	3 cut(s)	861, 1557, 2168
Bpu14I	TTCGAA	1 cut(s)	1675
BpuEI	CTTGAG	3 cut(s)	1163, 1499, 2038
BpuMI	CCSGG	7 cut(s)	430, 476, 515, 683, 1070, 1845, 1846
BsaAI	YACGTR	1 cut(s)	112
BsaHI	GRCGYC	5 cut(s)	405, 417, 555, 1146, 1568
BsaI	GGTCTC	1 cut(s)	473
BsaWI	WCCGGW	4 cut(s)	150, 464, 1097, 2272
BsaXI	ACNNNNNCTCC	6 cut(s)	224, 254, 309, 339, 647, 677
Bsc4I	CCNNNNNNNGG	5 cut(s)	150, 435, 493, 979, 1155
Bse118I	RCCGGY	5 cut(s)	5, 674, 953, 1265, 1450
Bse1I	ACTGG	2 cut(s)	1567, 1948
Bse3DI	GCAATG	4 cut(s)	1234, 1659, 1667, 2080
BseBI	CCWGG	4 cut(s)	1196, 1536, 1983, 2036
BseGI	GGATG	3 cut(s)	171, 254, 2086
BseLI	CCNNNNNNNGG	5 cut(s)	150, 435, 493, 979, 1155
BseMI	GCAATG	4 cut(s)	1234, 1659, 1667, 2080
BseMII	CTCAG	2 cut(s)	275, 2165
BseNI	ACTGG	2 cut(s)	1567, 1948
BseRI	GAGGAG	7 cut(s)	26, 133, 197, 266, 520, 2214, 2281
BseSI	GKGCMC	4 cut(s)	500, 514, 969, 1646
BseXI	GCAGC	6 cut(s)	43, 907, 1481, 1651, 1690, 2304
BseYI	CCCAGC	5 cut(s)	270, 344, 924, 1042, 1339
Bsh1236I	CGCG	4 cut(s)	225, 717, 1221, 1253
Bsh1285I	CGRYCG	5 cut(s)	88, 223, 687, 871, 889
BshNI	GGYRCC	2 cut(s)	497, 554
BsiEI	CGRYCG	5 cut(s)	88, 223, 687, 871, 889
BsiHKAI	GWGCWC	3 cut(s)	1837, 1895, 2024
BsiHKCI	CYCGRG	4 cut(s)	448, 1274, 1844, 2137
BsiWI	CGTACG	2 cut(s)	708, 882
BslFI	GGGAC	5 cut(s)	172, 469, 664, 1456, 1642
BslI	CCNNNNNNNGG	5 cut(s)	150, 435, 493, 979, 1155
BsmAI	GTCTC	5 cut(s)	473, 627, 885, 946, 1137
BsmBI	CGTCTC	2 cut(s)	946, 1137
BsmFI	GGGAC	5 cut(s)	172, 469, 664, 1456, 1642
Bso31I	GGTCTC	1 cut(s)	473
BsoBI	CYCGRG	4 cut(s)	448, 1274, 1844, 2137
Bsp119I	TTCGAA	1 cut(s)	1675
Bsp120I	GGGCCC	1 cut(s)	965
Bsp1286I	GDGCHC	9 cut(s)	500, 514, 969, 1177, 1275, 1646, 1837, 1895, 2024
Bsp19I	CCATGG	2 cut(s)	646, 998
BspCNI	CTCAG	2 cut(s)	274, 2166
BspFNI	CGCG	4 cut(s)	225, 717, 1221, 1253
BspLI	GGNNCC	9 cut(s)	10, 480, 499, 556, 679, 862, 910, 967, 1458
BspMI	ACCTGC	2 cut(s)	509, 1146
BspOI	GCTAGC	1 cut(s)	1265
BspPI	GGATC	9 cut(s)	61, 440, 822, 903, 916, 1589, 1776, 1817, 2034
BspQI	GCTCTTC	1 cut(s)	1305
BspT104I	TTCGAA	1 cut(s)	1675
BspT107I	GGYRCC	2 cut(s)	497, 554
BspTNI	GGTCTC	1 cut(s)	473
BsrBI	CCGCTC	1 cut(s)	1656
BsrDI	GCAATG	4 cut(s)	1234, 1659, 1667, 2080
BsrFI	RCCGGY	5 cut(s)	5, 674, 953, 1265, 1450
BsrI	ACTGG	2 cut(s)	1567, 1948
BssAI	RCCGGY	5 cut(s)	5, 674, 953, 1265, 1450
BssNAI	GTATAC	1 cut(s)	2304
BssNI	GRCGYC	5 cut(s)	405, 417, 555, 1146, 1568
BssT1I	CCWWGG	4 cut(s)	646, 741, 998, 1910
Bst1107I	GTATAC	1 cut(s)	2304
Bst2UI	CCWGG	4 cut(s)	1196, 1536, 1983, 2036
Bst4CI	ACNGT	8 cut(s)	89, 1012, 1027, 1163, 1435, 1907, 1970, 2252
Bst6I	CTCTTC	2 cut(s)	143, 1305
BstACI	GRCGYC	5 cut(s)	405, 417, 555, 1146, 1568
BstBAI	YACGTR	1 cut(s)	112
BstBI	TTCGAA	1 cut(s)	1675
BstDEI	CTNAG	3 cut(s)	261, 1930, 2174
BstDSI	CCRYGG	3 cut(s)	232, 646, 998
BstF5I	GGATG	3 cut(s)	171, 254, 2086
BstFNI	CGCG	4 cut(s)	225, 717, 1221, 1253
BstH2I	RGCGCY	3 cut(s)	448, 558, 1020
BstHHI	GCGC	6 cut(s)	61, 227, 447, 557, 1019, 1253
BstMAI	GTCTC	5 cut(s)	473, 627, 885, 946, 1137
BstMCI	CGRYCG	5 cut(s)	88, 223, 687, 871, 889
BstMWI	GCNNNNNNNGC	7 cut(s)	313, 322, 354, 668, 743, 752, 1295
BstNI	CCWGG	4 cut(s)	1196, 1536, 1983, 2036
BstNSI	RCATGY	2 cut(s)	1652, 1820
BstPAI	GACNNNNGTC	1 cut(s)	465
BstSFI	CTRYAG	1 cut(s)	1434
BstSLI	GKGCMC	4 cut(s)	500, 514, 969, 1646
BstUI	CGCG	4 cut(s)	225, 717, 1221, 1253
BstV1I	GCAGC	6 cut(s)	43, 907, 1481, 1651, 1690, 2304
BstX2I	RGATCY	3 cut(s)	432, 908, 1768
BstXI	CCANNNNNNTGG	2 cut(s)	733, 1585
BstYI	RGATCY	3 cut(s)	432, 908, 1768
BstZ17I	GTATAC	1 cut(s)	2304
BtgI	CCRYGG	3 cut(s)	232, 646, 998
BtrI	CACGTC	1 cut(s)	320
BtsCI	GGATG	3 cut(s)	171, 254, 2086
BtsIMutI	CAGTG	2 cut(s)	514, 2161
BveI	ACCTGC	2 cut(s)	509, 1146
CaiI	CAGNNNCTG	2 cut(s)	1161, 2173
CfoI	GCGC	6 cut(s)	61, 227, 447, 557, 1019, 1253
Cfr10I	RCCGGY	5 cut(s)	5, 674, 953, 1265, 1450
Cfr13I	GGNCC	6 cut(s)	381, 473, 965, 966, 1456, 1487
Cfr9I	CCCGGG	1 cut(s)	1844
CseI	GACGC	4 cut(s)	406, 493, 1154, 1557
Csp6I	GTAC	7 cut(s)	160, 709, 883, 1093, 1159, 1903, 2044
CspCI	CAANNNNNGTGG	2 cut(s)	1332, 1367
CviAII	CATG	8 cut(s)	647, 733, 929, 999, 1649, 1805, 1817, 2314
CviQI	GTAC	7 cut(s)	160, 709, 883, 1093, 1159, 1903, 2044
DdeI	CTNAG	3 cut(s)	261, 1930, 2174
DinI	GGCGCC	1 cut(s)	556
DrdI	GACNNNNNNGTC	4 cut(s)	227, 410, 1567, 1636
DriI	GACNNNNNGTC	1 cut(s)	523
DseDI	GACNNNNNNGTC	4 cut(s)	227, 410, 1567, 1636
EaeI	YGGCCR	7 cut(s)	3, 437, 735, 804, 981, 1287, 1410
Eam1104I	CTCTTC	2 cut(s)	143, 1305
Eam1105I	GACNNNNNGTC	1 cut(s)	523
EarI	CTCTTC	2 cut(s)	143, 1305
EciI	GGCGGA	5 cut(s)	232, 934, 940, 1155, 2232
Ecl136II	GAGCTC	2 cut(s)	1835, 2022
Eco130I	CCWWGG	4 cut(s)	646, 741, 998, 1910
Eco147I	AGGCCT	1 cut(s)	1682
Eco24I	GRGCYC	5 cut(s)	969, 1177, 1275, 1837, 2024
Eco31I	GGTCTC	1 cut(s)	473
Eco47I	GGWCC	1 cut(s)	1487
Eco53kI	GAGCTC	2 cut(s)	1835, 2022
Eco72I	CACGTG	1 cut(s)	112
Eco88I	CYCGRG	4 cut(s)	448, 1274, 1844, 2137
EcoICRI	GAGCTC	2 cut(s)	1835, 2022
EcoRI	GAATTC	1 cut(s)	1492
EcoRII	CCWGG	4 cut(s)	1194, 1534, 1981, 2034
EcoT14I	CCWWGG	4 cut(s)	646, 741, 998, 1910
EcoT38I	GRGCYC	5 cut(s)	969, 1177, 1275, 1837, 2024
EgeI	GGCGCC	1 cut(s)	556
EheI	GGCGCC	1 cut(s)	556
ErhI	CCWWGG	4 cut(s)	646, 741, 998, 1910
Esp3I	CGTCTC	2 cut(s)	946, 1137
FaeI	CATG	8 cut(s)	650, 736, 932, 1002, 1652, 1808, 1820, 2317
FaqI	GGGAC	5 cut(s)	172, 469, 664, 1456, 1642
FatI	CATG	8 cut(s)	646, 732, 928, 998, 1648, 1804, 1816, 2313
FauI	CCCGC	6 cut(s)	78, 139, 295, 1262, 1546, 1653
FbaI	TGATCA	1 cut(s)	1722
FblI	GTMKAC	2 cut(s)	1790, 2303
FokI	GGATG	3 cut(s)	158, 241, 2093
FriOI	GRGCYC	5 cut(s)	969, 1177, 1275, 1837, 2024
FspBI	CTAG	4 cut(s)	1109, 1262, 1599, 1977
GlaI	GCGC	6 cut(s)	60, 226, 446, 556, 1018, 1252
GsaI	CCCAGC	5 cut(s)	274, 348, 928, 1046, 1343
GsuI	CTGGAG	3 cut(s)	861, 1557, 2168
HaeII	RGCGCY	3 cut(s)	448, 558, 1020
HgaI	GACGC	4 cut(s)	406, 493, 1154, 1557
HhaI	GCGC	6 cut(s)	61, 227, 447, 557, 1019, 1253
Hin1I	GRCGYC	5 cut(s)	405, 417, 555, 1146, 1568
Hin1II	CATG	8 cut(s)	650, 736, 932, 1002, 1652, 1808, 1820, 2317
Hin6I	GCGC	6 cut(s)	59, 225, 445, 555, 1017, 1251
HinP1I	GCGC	6 cut(s)	59, 225, 445, 555, 1017, 1251
HincII	GTYRAC	3 cut(s)	838, 1030, 1791
HindII	GTYRAC	3 cut(s)	838, 1030, 1791
HphI	GGTGA	8 cut(s)	140, 1109, 1112, 1267, 1367, 1997, 2107, 2275
Hpy166II	GTNNAC	9 cut(s)	109, 162, 232, 838, 1030, 1543, 1791, 2244, 2304
Hpy188III	TCNNGA	5 cut(s)	64, 486, 890, 2017, 2194
Hpy8I	GTNNAC	9 cut(s)	109, 162, 232, 838, 1030, 1543, 1791, 2244, 2304
Hpy99I	CGWCG	6 cut(s)	404, 407, 575, 833, 860, 1411
HpyAV	CCTTC	8 cut(s)	29, 34, 109, 379, 487, 635, 922, 2010
HpyCH4III	ACNGT	8 cut(s)	89, 1012, 1027, 1163, 1435, 1907, 1970, 2252
HpyCH4IV	ACGT	3 cut(s)	111, 319, 405
HpyCH4V	TGCA	6 cut(s)	537, 1328, 1667, 1775, 1883, 1959
HpyF10VI	GCNNNNNNNGC	7 cut(s)	313, 322, 354, 668, 743, 752, 1295
HpyF3I	CTNAG	3 cut(s)	261, 1930, 2174
HpySE526I	ACGT	3 cut(s)	111, 319, 405
Hsp92I	GRCGYC	5 cut(s)	405, 417, 555, 1146, 1568
Hsp92II	CATG	8 cut(s)	650, 736, 932, 1002, 1652, 1808, 1820, 2317
HspAI	GCGC	6 cut(s)	59, 225, 445, 555, 1017, 1251
KasI	GGCGCC	1 cut(s)	554
KroI	GCCGGC	3 cut(s)	674, 1265, 1450
KroNI	GCCGGC	3 cut(s)	676, 1267, 1452
Ksp22I	TGATCA	1 cut(s)	1722
LguI	GCTCTTC	1 cut(s)	1305
Lsp1109I	GCAGC	6 cut(s)	43, 907, 1481, 1651, 1690, 2304
LweI	GCATC	2 cut(s)	1315, 2071
MaeI	CTAG	4 cut(s)	1109, 1262, 1599, 1977
MaeII	ACGT	3 cut(s)	111, 319, 405
MaeIII	GTNAC	4 cut(s)	301, 1163, 2101, 2185
MbiI	CCGCTC	1 cut(s)	1656
MboII	GAAGA	4 cut(s)	130, 1322, 1614, 2171
MfeI	CAATTG	2 cut(s)	822, 1416
MflI	RGATCY	3 cut(s)	432, 908, 1768
MhlI	GDGCHC	9 cut(s)	500, 514, 969, 1177, 1275, 1646, 1837, 1895, 2024
MlsI	TGGCCA	2 cut(s)	737, 1289
MluCI	AATT	9 cut(s)	822, 843, 958, 1203, 1416, 1492, 1620, 1690, 1993
MluNI	TGGCCA	2 cut(s)	737, 1289
Mly113I	GGCGCC	1 cut(s)	555
MlyI	GAGTC	9 cut(s)	455, 626, 887, 1245, 1786, 1796, 2061, 2179, 2278
MmeI	TCCRAC	7 cut(s)	36, 108, 414, 819, 838, 1219, 1538
Mox20I	TGGCCA	2 cut(s)	737, 1289
MreI	CGCCGGCG	1 cut(s)	1450
MroNI	GCCGGC	3 cut(s)	674, 1265, 1450
MroXI	GAANNNNTTC	1 cut(s)	1314
MscI	TGGCCA	2 cut(s)	737, 1289
MseI	TTAA	3 cut(s)	599, 1130, 2040
MslI	CAYNNNNRTG	2 cut(s)	781, 1888
Msp20I	TGGCCA	2 cut(s)	737, 1289
MspA1I	CMGCKG	2 cut(s)	867, 1305
MunI	CAATTG	2 cut(s)	822, 1416
MvaI	CCWGG	4 cut(s)	1196, 1536, 1983, 2036
MvnI	CGCG	4 cut(s)	225, 717, 1221, 1253
MwoI	GCNNNNNNNGC	7 cut(s)	313, 322, 354, 668, 743, 752, 1295
NaeI	GCCGGC	3 cut(s)	676, 1267, 1452
NarI	GGCGCC	1 cut(s)	555
NciI	CCSGG	7 cut(s)	430, 476, 515, 683, 1070, 1845, 1846
NcoI	CCATGG	2 cut(s)	646, 998
NgoMIV	GCCGGC	3 cut(s)	674, 1265, 1450
NheI	GCTAGC	1 cut(s)	1261
NlaIII	CATG	8 cut(s)	650, 736, 932, 1002, 1652, 1808, 1820, 2317
NlaIV	GGNNCC	9 cut(s)	10, 480, 499, 556, 679, 862, 910, 967, 1458
NmeAIII	GCCGAG	3 cut(s)	333, 450, 1038
NmuCI	GTSAC	1 cut(s)	301
NspI	RCATGY	2 cut(s)	1652, 1820
NspV	TTCGAA	1 cut(s)	1675
PaqCI	CACCTGC	1 cut(s)	509
PceI	AGGCCT	1 cut(s)	1682
PciI	ACATGT	1 cut(s)	1816
PciSI	GCTCTTC	1 cut(s)	1305
PcsI	WCGNNNNNNNCGW	6 cut(s)	225, 402, 420, 576, 617, 717
PdiI	GCCGGC	3 cut(s)	676, 1267, 1452
PdmI	GAANNNNTTC	1 cut(s)	1314
PfeI	GAWTC	7 cut(s)	365, 608, 750, 791, 1003, 1510, 1748
Pfl23II	CGTACG	2 cut(s)	708, 882
PflFI	GACNNNGTC	1 cut(s)	404
PfoI	TCCNGGA	1 cut(s)	1534
Ple19I	CGATCG	1 cut(s)	889
PleI	GAGTC	9 cut(s)	455, 625, 887, 1244, 1786, 1795, 2060, 2178, 2277
PluTI	GGCGCC	1 cut(s)	558
PmaCI	CACGTG	1 cut(s)	112
PmlI	CACGTG	1 cut(s)	112
PpsI	GAGTC	9 cut(s)	455, 625, 887, 1244, 1786, 1795, 2060, 2178, 2277
Ppu21I	YACGTR	1 cut(s)	112
PscI	ACATGT	1 cut(s)	1816
PshAI	GACNNNNGTC	1 cut(s)	465
PshBI	ATTAAT	2 cut(s)	599, 1130
PsiI	TTATAA	1 cut(s)	2091
Psp124BI	GAGCTC	2 cut(s)	1837, 2024
Psp6I	CCWGG	4 cut(s)	1194, 1534, 1981, 2034
PspCI	CACGTG	1 cut(s)	112
PspFI	CCCAGC	5 cut(s)	270, 344, 924, 1042, 1339
PspGI	CCWGG	4 cut(s)	1194, 1534, 1981, 2034
PspLI	CGTACG	2 cut(s)	708, 882
PspN4I	GGNNCC	9 cut(s)	10, 480, 499, 556, 679, 862, 910, 967, 1458
PspOMI	GGGCCC	1 cut(s)	965
PspPI	GGNCC	6 cut(s)	381, 473, 965, 966, 1456, 1487
PstNI	CAGNNNCTG	2 cut(s)	1161, 2173
PsuI	RGATCY	3 cut(s)	432, 908, 1768
PsyI	GACNNNGTC	1 cut(s)	404
PvuI	CGATCG	1 cut(s)	889
RsaI	GTAC	7 cut(s)	161, 710, 884, 1094, 1160, 1904, 2045
RsaNI	GTAC	7 cut(s)	160, 709, 883, 1093, 1159, 1903, 2044
RseI	CAYNNNNRTG	2 cut(s)	781, 1888
SacI	GAGCTC	2 cut(s)	1837, 2024
SalI	GTCGAC	1 cut(s)	1789
SapI	GCTCTTC	1 cut(s)	1305
SaqAI	TTAA	3 cut(s)	599, 1130, 2040
Sau96I	GGNCC	6 cut(s)	381, 473, 965, 966, 1456, 1487
SchI	GAGTC	9 cut(s)	455, 626, 887, 1245, 1786, 1796, 2061, 2179, 2278
SduI	GDGCHC	9 cut(s)	500, 514, 969, 1177, 1275, 1646, 1837, 1895, 2024
SfaNI	GCATC	2 cut(s)	1315, 2071
SfcI	CTRYAG	1 cut(s)	1434
SfoI	GGCGCC	1 cut(s)	556
SfuI	TTCGAA	1 cut(s)	1675
SgrAI	CRCCGGYG	1 cut(s)	1450
SinI	GGWCC	1 cut(s)	1487
SmaI	CCCGGG	1 cut(s)	1846
SmiMI	CAYNNNNRTG	2 cut(s)	781, 1888
SmlI	CTYRAG	3 cut(s)	1178, 1514, 2017
SmoI	CTYRAG	3 cut(s)	1178, 1514, 2017
Sse9I	AATT	9 cut(s)	822, 843, 958, 1203, 1416, 1492, 1620, 1690, 1993
SseBI	AGGCCT	1 cut(s)	1682
SspDI	GGCGCC	1 cut(s)	554
SspMI	CTAG	4 cut(s)	1109, 1262, 1599, 1977
SstI	GAGCTC	2 cut(s)	1837, 2024
StuI	AGGCCT	1 cut(s)	1682
StyI	CCWWGG	4 cut(s)	646, 741, 998, 1910
TaaI	ACNGT	8 cut(s)	89, 1012, 1027, 1163, 1435, 1907, 1970, 2252
TaiI	ACGT	3 cut(s)	114, 322, 408
TaqII	GACCGA	1 cut(s)	1504
TasI	AATT	9 cut(s)	822, 843, 958, 1203, 1416, 1492, 1620, 1690, 1993
TatI	WGTACW	2 cut(s)	1092, 2043
TauI	GCSGC	9 cut(s)	248, 674, 720, 809, 1224, 1227, 1367, 1457, 1659
TfiI	GAWTC	7 cut(s)	365, 608, 750, 791, 1003, 1510, 1748
Tru1I	TTAA	3 cut(s)	599, 1130, 2040
Tru9I	TTAA	3 cut(s)	599, 1130, 2040
TscAI	CASTG	2 cut(s)	514, 2161
TseFI	GTSAC	1 cut(s)	301
TseI	GCWGC	6 cut(s)	56, 920, 1469, 1664, 1703, 2292
Tsp45I	GTSAC	1 cut(s)	301
TspDTI	ATGAA	3 cut(s)	91, 1077, 1793
TspGWI	ACGGA	4 cut(s)	289, 471, 844, 1999
TspMI	CCCGGG	1 cut(s)	1844
TspRI	CASTG	2 cut(s)	514, 2161
Tth111I	GACNNNGTC	1 cut(s)	404
VpaK11BI	GGWCC	1 cut(s)	1487
VspI	ATTAAT	2 cut(s)	599, 1130
XapI	RAATTY	1 cut(s)	1492
XceI	RCATGY	2 cut(s)	1652, 1820
XmaI	CCCGGG	1 cut(s)	1844
XmiI	GTMKAC	2 cut(s)	1790, 2303
XmnI	GAANNNNTTC	1 cut(s)	1314
XspI	CTAG	4 cut(s)	1109, 1262, 1599, 1977
ZraI	GACGTC	1 cut(s)	406

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.