Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0005080

Physical Location & Seq

Genomic Coordinates Reverse (-)

16417301 .. 16421263

Gene Structure 3963 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1188000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1635 bp

ATGTCAAATGTTACAAAGATAAGGAGATTGATGGAAGACATGTCTCCAGCAGTTGCAGTGACTCTTAGTTTAGGTAATGCTATATGTGATAGTGCCGGAATTGGAGCCAATGTGGAGTTCACATGGCTAAAGCTTGTAACAGACTTGGGAAACTTGTCTTCAGATTCCTCCAAGGTGATTCCTTTAGAGTCAGTCGCTTGCAGTAAAGGAAGTTCCGATGATATTGAAAGCAAAGTTAGTGTGGTTCCCGTGCCATCACGAGACGATGATGGTGGAAACACTTCTGTTGCTAATAATGTCATGGTTCAAGAAAGTGAGGAAGAGCAAATCTTAGCCTCTAGATTTGATACTAACGGAATTGTTAGTGAGGAAGTGCTGAAGTTAGAGATGGGGTCTGCGATAAGCTTGCCAGATGATATGGAGATTGGAAAAATTAGTGAAGGGAAGATTGTTGCAAAAGCTATTGTCTTGGTAGAATCGGCTGTTGGTAAAATGGCTTCTGGATTTGTTGCAGCAGTAAGCCCCGCTTCTGAGTTATCAGATAAATCTGAATTATCAACCTCTACGGTACTTATCCACGCAAATGGTGAGAAGATTGTCAGTAAAGCAGGCAACCGGAGTGTTTTTGAGCTAAACTGTATTCCCCTCTGGGGTTCTGTATCAATTTGTGGAAGACGACCAGAAATGGAAGATGCATTTGCTGCTGTTCCTCGGTGTATCAATATTCCAATCAAAATGCTAGCCAGTAATCAAGTGTATAATGGAATGAGCCAAAGTTTGACCCACCTAACCAGTCACTTTTTTGGCATTTACGATGGCCACGGCGGCCCTCAGGTTGCTAACTTTTGTAGTGAGCGGCTCCATTTGGCTTTGGCCGAAGAGCTTGGAGTCATTAAGGATGACTCTGGTGATGGGATTACGGGAGAAACTCAGCAGTTGCATTGGGAGAAAGCATTCACTAATTGTTTTCAAAGGGTTGATGATGAGATTGGAGGAAAAGTAAGCAGAGGCATCATTGAAAGCAACACAGATGCCTCTGAAGTTAGTTTTGAGCCTATTGCCCCAGAAACTGTTGGGTCTACAGCTGTGGTTGCATTAGTTTCGTCATCCCATATTATAGTTGCAAATTGTGGTGATTCAAGAGCAGTTCTATGTCGTGGCAAACAAGCAATACCATTATCAGTCGATCATAAACCAAACAGAGAAGACGAATATGAAAGGATTGAGGCATCTGGCGGCAAGGTAATACAATGGAATGGGCACCGTGTTTTTGGAGTTCTTGCAATGTCAAGGTCCATTGGGGATAGATATTTGAAACCGTGGATAATTCCAGAACCAGAAGTCATGGTTGTCCCTCGAGCTAGAGATGACGAATGCCTTATTTTAGCTAGTGATGGTCTATGGGATGTTATGACGAATGAGGAAGCTTGTGATGTGGCTCGAAAACGCATTCTGCTATGGCACAAAAAGAACGGGGCTACACCTCTTGCGGAAAGGGGAACGGGAGTTGATCCTGCGGCTGGGGAAGCTGCTGCCTACCTTGCAACCCTTGCCCTCCAGAAGGGAAGCAAGGACAACATCTCTGTGGTTGTGGTGGACTTGAAAGCTCAAAGGAAGTTCAAGAGCAAATCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010035 GO:0010646 GO:0010648 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0022622 GO:0023051 GO:0023057 GO:0032501 GO:0032502 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0065007 GO:0071704 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 ko04931 map04016 map04075 map04931

Pfam Domains

Protein Families

PP2C

Protein Analysis

545

Amino Acids

58.24

Weight (kDa)

5.06

Isoelectric Point (pI)

42.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	244 - 527	1.8e-62	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005080)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17550 AT1G17550 AT1G72770 AT1G72770 AT1G72770 AT1G72770 AT1G72770
fragaria_vesca	FvH4_6g34170 FvH4_6g34170 FvH4_6g34170 FvH4_6g34170
malus_domestica	MD09G1188000.v1.1 MD17G1172400.v1.1
prunus_persica	Prupe.3G044900_v2.0.a1 Prupe.3G044900_v2.0.a1 Prupe.3G044900_v2.0.a1
pyrus_communis	pycom09g10440 pycom17g17010
rosa_chinensis	RchiOBHm_Chr2g0144521
rosa_laevigata	RLG00000020085
rosa_multiflora	Rmu_sc0005789.1_g000006 Rmu_sc0009947.1_g000001
rosa_roxburghii	Rroxscaffold_2G00101240
rosa_rugosa	Rorug02G0387400 Rorug02G0387500
rosa_samantha	Rh2AG439500 Rh2BG449600 Rh2CG426500 Rh2DG459500
rosa_wichuraiana	Rw2G035950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1260
AccBSI	CCGCTC	1 cut(s)	856
AccI	GTMKAC	1 cut(s)	1079
AciI	CCGC	6 cut(s)	525, 825, 856, 1236, 1490, 1517
AclWI	GGATC	1 cut(s)	1505
AcoI	YGGCCR	2 cut(s)	817, 873
AcuI	CTGAAG	3 cut(s)	144, 398, 1059
AfaI	GTAC	1 cut(s)	570
AfiI	CCNNNNNNNGG	3 cut(s)	650, 1520, 1561
AflIII	ACRYGT	1 cut(s)	39
AgsI	TTSAA	8 cut(s)	227, 308, 971, 1019, 1140, 1315, 1603, 1621
AjuI	GAANNNNNNNTTGG	4 cut(s)	101, 133, 468, 500
Alw26I	GTCTC	2 cut(s)	48, 255
AlwI	GGATC	1 cut(s)	1505
AlwNI	CAGNNNCTG	1 cut(s)	1070
Ama87I	CYCGRG	1 cut(s)	1356
AoxI	GGCC	3 cut(s)	817, 826, 873
ApeKI	GCWGC	4 cut(s)	512, 701, 1529, 1532
Asp700I	GAANNNNTTC	2 cut(s)	280, 953
AspS9I	GGNCC	2 cut(s)	827, 1293
AsuHPI	GGTGA	4 cut(s)	187, 599, 920, 1145
AsuNHI	GCTAGC	1 cut(s)	739
AvaI	CYCGRG	1 cut(s)	1356
AvaII	GGWCC	1 cut(s)	1293
AxyI	CCTNAGG	1 cut(s)	831
BaeGI	GKGCMC	1 cut(s)	1263
BalI	TGGCCA	1 cut(s)	819
BanI	GGYRCC	1 cut(s)	1260
BarI	GAAGNNNNNNTAC	2 cut(s)	481, 513
BauI	CACGAG	1 cut(s)	258
BbsI	GAAGAC	4 cut(s)	42, 150, 679, 1212
BbvI	GCAGC	4 cut(s)	524, 688, 1516, 1519
BccI	CCATC	7 cut(s)	25, 262, 263, 382, 809, 905, 1388
BceAI	ACGGC	1 cut(s)	838
BcgI	CGANNNNNNTGC	4 cut(s)	388, 422, 1083, 1117
BcoDI	GTCTC	2 cut(s)	48, 255
BfaI	CTAG	4 cut(s)	339, 740, 1362, 1389
BfmI	CTRYAG	1 cut(s)	1080
BglI	GCCNNNNNGGC	1 cut(s)	825
BisI	GCNGC	8 cut(s)	513, 702, 826, 857, 1237, 1518, 1530, 1533
BlsI	GCNGC	8 cut(s)	514, 703, 827, 858, 1238, 1519, 1531, 1534
Bme18I	GGWCC	1 cut(s)	1293
BmeT110I	CYCGRG	1 cut(s)	1356
BmgT120I	GGNCC	2 cut(s)	827, 1293
BmiI	GGNNCC	4 cut(s)	106, 246, 860, 1262
BmsI	GCATC	4 cut(s)	682, 1020, 1021, 1238
BmtI	GCTAGC	1 cut(s)	743
BpiI	GAAGAC	4 cut(s)	42, 150, 679, 1212
BpmI	CTGGAG	2 cut(s)	30, 1541
BsaJI	CCNNGG	4 cut(s)	171, 710, 820, 1319
BsaWI	WCCGGW	1 cut(s)	615
BsaXI	ACNNNNNCTCC	4 cut(s)	96, 126, 984, 1014
Bsc4I	CCNNNNNNNGG	3 cut(s)	650, 1520, 1561
Bse1I	ACTGG	2 cut(s)	744, 792
Bse21I	CCTNAGG	1 cut(s)	831
Bse3DI	GCAATG	1 cut(s)	1290
BseDI	CCNNGG	4 cut(s)	171, 710, 820, 1319
BseGI	GGATG	3 cut(s)	904, 1106, 1411
BseLI	CCNNNNNNNGG	3 cut(s)	650, 1520, 1561
BseMI	GCAATG	1 cut(s)	1290
BseMII	CTCAG	3 cut(s)	522, 845, 944
BseNI	ACTGG	2 cut(s)	744, 792
BseSI	GKGCMC	1 cut(s)	1263
BseXI	GCAGC	4 cut(s)	524, 688, 1516, 1519
BseYI	CCCAGC	1 cut(s)	1520
BshFI	GGCC	3 cut(s)	819, 828, 875
BshNI	GGYRCC	1 cut(s)	1260
BsiHKCI	CYCGRG	1 cut(s)	1356
BsiSI	CCGG	2 cut(s)	96, 616
BslFI	GGGAC	1 cut(s)	1337
BslI	CCNNNNNNNGG	3 cut(s)	650, 1520, 1561
BsmAI	GTCTC	2 cut(s)	48, 255
BsmBI	CGTCTC	1 cut(s)	255
BsmFI	GGGAC	1 cut(s)	1337
BsmI	GAATGC	3 cut(s)	953, 1379, 1449
BsnI	GGCC	3 cut(s)	819, 828, 875
BsoBI	CYCGRG	1 cut(s)	1356
Bsp1286I	GDGCHC	1 cut(s)	1263
Bsp143I	GATC	2 cut(s)	1186, 1510
BspACI	CCGC	6 cut(s)	525, 825, 856, 1236, 1490, 1517
BspANI	GGCC	3 cut(s)	819, 828, 875
BspCNI	CTCAG	3 cut(s)	523, 844, 943
BspLI	GGNNCC	4 cut(s)	106, 246, 860, 1262
BspOI	GCTAGC	1 cut(s)	743
BspPI	GGATC	1 cut(s)	1505
BspQI	GCTCTTC	2 cut(s)	315, 873
BspT107I	GGYRCC	1 cut(s)	1260
BsrBI	CCGCTC	1 cut(s)	856
BsrDI	GCAATG	1 cut(s)	1290
BsrI	ACTGG	2 cut(s)	744, 792
BssECI	CCNNGG	4 cut(s)	171, 710, 820, 1319
BssMI	GATC	2 cut(s)	1186, 1510
BssSI	CACGAG	1 cut(s)	258
BssT1I	CCWWGG	1 cut(s)	171
Bst2BI	CACGAG	1 cut(s)	258
Bst4CI	ACNGT	5 cut(s)	568, 638, 1072, 1265, 1320
Bst6I	CTCTTC	2 cut(s)	315, 873
BstAPI	GCANNNNNTGC	2 cut(s)	701, 1550
BstC8I	GCNNGC	4 cut(s)	199, 407, 610, 741
BstDEI	CTNAG	5 cut(s)	65, 331, 531, 831, 930
BstDSI	CCRYGG	2 cut(s)	820, 1319
BstENI	CCTNNNNNAGG	1 cut(s)	1559
BstF5I	GGATG	3 cut(s)	904, 1106, 1411
BstKTI	GATC	2 cut(s)	1189, 1513
BstMAI	GTCTC	2 cut(s)	48, 255
BstMBI	GATC	2 cut(s)	1186, 1510
BstMWI	GCNNNNNNNGC	6 cut(s)	701, 825, 1091, 1526, 1541, 1550
BstNSI	RCATGY	1 cut(s)	43
BstSFI	CTRYAG	1 cut(s)	1080
BstSLI	GKGCMC	1 cut(s)	1263
BstV1I	GCAGC	4 cut(s)	524, 688, 1516, 1519
BstV2I	GAAGAC	4 cut(s)	42, 150, 679, 1212
BstXI	CCANNNNNNTGG	1 cut(s)	584
Bsu36I	CCTNAGG	1 cut(s)	831
BsuRI	GGCC	3 cut(s)	819, 828, 875
BtgI	CCRYGG	2 cut(s)	820, 1319
BtsCI	GGATG	3 cut(s)	904, 1106, 1411
BtsI	GCAGTG	1 cut(s)	63
BtsIMutI	CAGTG	1 cut(s)	63
Cac8I	GCNNGC	4 cut(s)	199, 407, 610, 741
CaiI	CAGNNNCTG	1 cut(s)	1070
Cfr13I	GGNCC	2 cut(s)	827, 1293
Csp6I	GTAC	1 cut(s)	569
CviAII	CATG	4 cut(s)	40, 123, 301, 1345
CviQI	GTAC	1 cut(s)	569
DdeI	CTNAG	5 cut(s)	65, 331, 531, 831, 930
DpnI	GATC	2 cut(s)	1188, 1512
DpnII	GATC	2 cut(s)	1186, 1510
EaeI	YGGCCR	2 cut(s)	817, 873
Eam1104I	CTCTTC	2 cut(s)	315, 873
EarI	CTCTTC	2 cut(s)	315, 873
Eco130I	CCWWGG	1 cut(s)	171
Eco47I	GGWCC	1 cut(s)	1293
Eco57I	CTGAAG	3 cut(s)	144, 398, 1059
Eco81I	CCTNAGG	1 cut(s)	831
Eco88I	CYCGRG	1 cut(s)	1356
EcoNI	CCTNNNNNAGG	1 cut(s)	1559
EcoT14I	CCWWGG	1 cut(s)	171
EcoT22I	ATGCAT	1 cut(s)	697
ErhI	CCWWGG	1 cut(s)	171
Esp3I	CGTCTC	1 cut(s)	255
FaeI	CATG	4 cut(s)	43, 126, 304, 1348
FalI	AAGNNNNNCTT	2 cut(s)	511, 543
FaqI	GGGAC	1 cut(s)	1337
FatI	CATG	4 cut(s)	39, 122, 300, 1344
FauI	CCCGC	1 cut(s)	532
FblI	GTMKAC	1 cut(s)	1079
Fnu4HI	GCNGC	8 cut(s)	513, 702, 826, 857, 1237, 1518, 1530, 1533
FokI	GGATG	3 cut(s)	911, 1093, 1418
Fsp4HI	GCNGC	8 cut(s)	513, 702, 826, 857, 1237, 1518, 1530, 1533
FspBI	CTAG	4 cut(s)	339, 740, 1362, 1389
GluI	GCNGC	8 cut(s)	513, 702, 826, 857, 1237, 1518, 1530, 1533
GsaI	CCCAGC	1 cut(s)	1524
GsuI	CTGGAG	2 cut(s)	30, 1541
HaeIII	GGCC	3 cut(s)	819, 828, 875
HapII	CCGG	2 cut(s)	96, 616
Hin1II	CATG	4 cut(s)	43, 126, 304, 1348
HindIII	AAGCTT	3 cut(s)	131, 403, 1425
HinfI	GANTC	8 cut(s)	61, 164, 178, 188, 476, 888, 902, 1136
HpaII	CCGG	2 cut(s)	96, 616
HphI	GGTGA	4 cut(s)	187, 599, 920, 1145
Hpy166II	GTNNAC	3 cut(s)	120, 1080, 1597
Hpy188I	TCNGA	6 cut(s)	163, 217, 532, 541, 550, 1039
Hpy188III	TCNNGA	8 cut(s)	258, 308, 339, 501, 1140, 1331, 1558, 1621
Hpy8I	GTNNAC	3 cut(s)	120, 1080, 1597
HpyAV	CCTTC	2 cut(s)	434, 1555
HpyCH4III	ACNGT	5 cut(s)	568, 638, 1072, 1265, 1320
HpyF10VI	GCNNNNNNNGC	6 cut(s)	701, 825, 1091, 1526, 1541, 1550
HpyF3I	CTNAG	5 cut(s)	65, 331, 531, 831, 930
Hsp92II	CATG	4 cut(s)	43, 126, 304, 1348
Kzo9I	GATC	2 cut(s)	1186, 1510
LguI	GCTCTTC	2 cut(s)	315, 873
LmnI	GCTCC	2 cut(s)	104, 864
Lsp1109I	GCAGC	4 cut(s)	524, 688, 1516, 1519
LweI	GCATC	4 cut(s)	682, 1020, 1021, 1238
MaeI	CTAG	4 cut(s)	339, 740, 1362, 1389
MaeIII	GTNAC	4 cut(s)	10, 58, 136, 794
MalI	GATC	2 cut(s)	1188, 1512
MbiI	CCGCTC	1 cut(s)	856
MboI	GATC	2 cut(s)	1186, 1510
MboII	GAAGA	9 cut(s)	47, 150, 332, 457, 604, 684, 701, 890, 1217
MhlI	GDGCHC	1 cut(s)	1263
MlsI	TGGCCA	1 cut(s)	819
MluCI	AATT	8 cut(s)	99, 357, 432, 551, 663, 961, 1126, 1326
MluNI	TGGCCA	1 cut(s)	819
MlyI	GAGTC	4 cut(s)	55, 197, 896, 897
Mox20I	TGGCCA	1 cut(s)	819
Mph1103I	ATGCAT	1 cut(s)	697
MroXI	GAANNNNTTC	2 cut(s)	280, 953
MscI	TGGCCA	1 cut(s)	819
MseI	TTAA	1 cut(s)	894
MslI	CAYNNNNRTG	2 cut(s)	582, 1583
Msp20I	TGGCCA	1 cut(s)	819
MspA1I	CMGCKG	1 cut(s)	1085
MspI	CCGG	2 cut(s)	96, 616
Mva1269I	GAATGC	3 cut(s)	953, 1379, 1449
MwoI	GCNNNNNNNGC	6 cut(s)	701, 825, 1091, 1526, 1541, 1550
NdeII	GATC	2 cut(s)	1186, 1510
NheI	GCTAGC	1 cut(s)	739
NlaIII	CATG	4 cut(s)	43, 126, 304, 1348
NlaIV	GGNNCC	4 cut(s)	106, 246, 860, 1262
NmuCI	GTSAC	2 cut(s)	58, 794
NsiI	ATGCAT	1 cut(s)	697
NspI	RCATGY	1 cut(s)	43
PaeR7I	CTCGAG	1 cut(s)	1356
PciI	ACATGT	1 cut(s)	39
PciSI	GCTCTTC	2 cut(s)	315, 873
PctI	GAATGC	3 cut(s)	953, 1379, 1449
PdmI	GAANNNNTTC	2 cut(s)	280, 953
PfeI	GAWTC	4 cut(s)	164, 178, 476, 1136
PkrI	GCNGC	8 cut(s)	514, 703, 827, 858, 1238, 1519, 1531, 1534
PleI	GAGTC	4 cut(s)	55, 196, 896, 896
PpsI	GAGTC	4 cut(s)	55, 196, 896, 896
PscI	ACATGT	1 cut(s)	39
PspFI	CCCAGC	1 cut(s)	1520
PspN4I	GGNNCC	4 cut(s)	106, 246, 860, 1262
PspPI	GGNCC	2 cut(s)	827, 1293
PspXI	VCTCGAGB	1 cut(s)	1356
PsrI	GAACNNNNNNTAC	2 cut(s)	196, 228
PstNI	CAGNNNCTG	1 cut(s)	1070
PvuII	CAGCTG	1 cut(s)	1085
RsaI	GTAC	1 cut(s)	570
RsaNI	GTAC	1 cut(s)	569
RseI	CAYNNNNRTG	2 cut(s)	582, 1583
SapI	GCTCTTC	2 cut(s)	315, 873
SaqAI	TTAA	1 cut(s)	894
SatI	GCNGC	8 cut(s)	513, 702, 826, 857, 1237, 1518, 1530, 1533
Sau3AI	GATC	2 cut(s)	1186, 1510
Sau96I	GGNCC	2 cut(s)	827, 1293
SchI	GAGTC	4 cut(s)	55, 197, 896, 897
SduI	GDGCHC	1 cut(s)	1263
SfaNI	GCATC	4 cut(s)	682, 1020, 1021, 1238
SfcI	CTRYAG	1 cut(s)	1080
SfiI	GGCCNNNNNGGCC	1 cut(s)	825
Sfr274I	CTCGAG	1 cut(s)	1356
SinI	GGWCC	1 cut(s)	1293
SlaI	CTCGAG	1 cut(s)	1356
SmiMI	CAYNNNNRTG	2 cut(s)	582, 1583
SmlI	CTYRAG	1 cut(s)	1356
SmoI	CTYRAG	1 cut(s)	1356
Sse9I	AATT	8 cut(s)	99, 357, 432, 551, 663, 961, 1126, 1326
SsiI	CCGC	6 cut(s)	525, 825, 856, 1236, 1490, 1517
SspI	AATATT	1 cut(s)	724
SspMI	CTAG	4 cut(s)	339, 740, 1362, 1389
StyI	CCWWGG	1 cut(s)	171
TaaI	ACNGT	5 cut(s)	568, 638, 1072, 1265, 1320
TaqI	TCGA	3 cut(s)	1185, 1357, 1441
TasI	AATT	8 cut(s)	99, 357, 432, 551, 663, 961, 1126, 1326
TauI	GCSGC	4 cut(s)	828, 859, 1239, 1520
TfiI	GAWTC	4 cut(s)	164, 178, 476, 1136
Tru1I	TTAA	1 cut(s)	894
Tru9I	TTAA	1 cut(s)	894
TscAI	CASTG	1 cut(s)	63
TseFI	GTSAC	2 cut(s)	58, 794
TseI	GCWGC	4 cut(s)	512, 701, 1529, 1532
Tsp45I	GTSAC	2 cut(s)	58, 794
TspDTI	ATGAA	1 cut(s)	1230
TspGWI	ACGGA	1 cut(s)	369
TspRI	CASTG	1 cut(s)	63
VpaK11BI	GGWCC	1 cut(s)	1293
XagI	CCTNNNNNAGG	1 cut(s)	1559
XbaI	TCTAGA	1 cut(s)	338
XceI	RCATGY	1 cut(s)	43
XhoI	CTCGAG	1 cut(s)	1356
XmiI	GTMKAC	1 cut(s)	1079
XmnI	GAANNNNTTC	2 cut(s)	280, 953
XspI	CTAG	4 cut(s)	339, 740, 1362, 1389
Zsp2I	ATGCAT	1 cut(s)	697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.