MD09G1192200.v1.1

Hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005743
Physical Location & Seq
Forward (+)
17132953 .. 17137353
4401 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1192200.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGGTTCAACTTTTGGGCACCATTCGCCCTTCTCCTTCTCTGAGCTACTTTGCAACATACAAACGCCAGCTCTCACTCTACAGCCCTCCAACCAAAGCTCCGGCAAATGGGTTTTCTCCCTTCAGACGAGTTTTCAACTCTTACCTCCAATCTAATTCAGAAACTACGGCCGCTGGCTCACCTTGTGAGCATTCAGAAATCATATTTATAGGTACTGGTACTAGTGAAGGAATTCCTCGAGTCAGCTGCCTCACAAATCCTTTAAAGACATGTGCGGTTTGCTTGAAAGCTGTGGAACCAGGGAATAAAAATAAGAGGCTCAACACAAGCATCCTTGTTCGTTATCCTAGACCCTCTGGAAATTATAACATTCTTATAGATGCTGGAAAGTTTTTCTACCACAGTGCTCTCCAATGGTTTACTGCCTATGGGATAAGAACAATTGATGCGGTTATTATTACTCATTCGCATGCTGATGCAATTGGAGGTCTGGATGATCTTCGCGATTGGACAAACAATGTCCAACCCAGCATCCCGATCTATGTTGCCCAGCGTGATTTTGAGGTAATGAAAAAGACTCATTATTATTTGGTGGATACAAGGGCGGTTCTTGCTGGTGCTGCAGTCTCTGAGTTGCAATTTAATATCATACGTGAGGATCCATTTGTAGTACAAGATTTGAAGTTTACCCCTTTACCTGTGTGGCATGGTCAGGGTTATCGTTCCCTTGGATTTCGTTTTGGTAATGTTTGTTATATAAGTGACGTTAGTGACATACCTGAAGAAACTTATCCACTTCTGAAAGATTGTGAACTCCTGATTCTGGATGCTTTACGGCCAGATCGGTCTTCTTCAACGCATTTTGGACTCCCAAGGGCTTTGGAGGAAGTACGGAAAATCCAACCGAGAAAGACTTTGTTCACTGGTATGATGCATCTGATGGATCATGAAAAGGTGAACGATTATCTTTTGAAATTGAAGGAGACAGAGGGTCTTGACGTACAGCTGAGCTATGACGGACTTCGTGTACCAGCAGCGTTTCACGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lactamase_B Lactamase_B_2

Protein Analysis

350

Amino Acids

39.21

Weight (kDa)

8.13

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anti-Pycsar_Apyc1 PF23023 108 - 275 3.7e-08 Anti-Pycsar protein Apyc1
Lactamase_B PF00753 109 - 179 9.3e-10 Metallo-beta-lactamase superfamily
Lactamase_B_2 PF12706 122 - 308 4.1e-24 Beta-lactamase superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 366
AccB1I GGYRCC 1 cut(s) 17
AccII CGCG 1 cut(s) 504
AciI CCGC 4 cut(s) 171, 275, 449, 605
AclWI GGATC 3 cut(s) 653, 666, 951
AcoI YGGCCR 2 cut(s) 168, 836
AcsI RAATTY 1 cut(s) 231
AcuI CTGAAG 2 cut(s) 106, 801
AdeI CACNNNGTG 1 cut(s) 185
AfaI GTAC 6 cut(s) 214, 220, 672, 891, 1002, 1029
AfiI CCNNNNNNNGG 2 cut(s) 107, 823
AflIII ACRYGT 1 cut(s) 269
AgsI TTSAA 7 cut(s) 8, 136, 286, 682, 855, 973, 979
AhdI GACNNNNNGTC 1 cut(s) 990
AhlI ACTAGT 1 cut(s) 221
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 7 cut(s) 45, 70, 98, 246, 290, 1006, 1011
AluI AGCT 7 cut(s) 45, 70, 98, 246, 290, 1006, 1011
Alw21I GWGCWC 1 cut(s) 409
Alw26I GTCTC 2 cut(s) 631, 977
AlwI GGATC 3 cut(s) 653, 666, 951
AlwNI CAGNNNCTG 1 cut(s) 629
Ama87I CYCGRG 1 cut(s) 237
AoxI GGCC 2 cut(s) 168, 836
ApeKI GCWGC 3 cut(s) 246, 620, 1034
ApoI RAATTY 1 cut(s) 231
Asp700I GAANNNNTTC 1 cut(s) 231
AsuHPI GGTGA 2 cut(s) 171, 967
AvaI CYCGRG 1 cut(s) 237
BaeGI GKGCMC 1 cut(s) 20
BamHI GGATCC 1 cut(s) 658
BanI GGYRCC 1 cut(s) 17
BbsI GAAGAC 1 cut(s) 840
Bbv12I GWGCWC 1 cut(s) 409
BbvI GCAGC 3 cut(s) 233, 607, 1046
BccI CCATC 1 cut(s) 934
BceAI ACGGC 2 cut(s) 183, 851
BcgI CGANNNNNNTGC 2 cut(s) 228, 262
BciT130I CCWGG 1 cut(s) 300
BciVI GTATCC 1 cut(s) 589
BcoDI GTCTC 2 cut(s) 631, 977
BcuI ACTAGT 1 cut(s) 221
BfaI CTAG 2 cut(s) 222, 348
BfmI CTRYAG 2 cut(s) 79, 621
BfuI GTATCC 1 cut(s) 589
BisI GCNGC 4 cut(s) 171, 247, 621, 1035
BlpI GCTNAGC 1 cut(s) 1007
BlsI GCNGC 4 cut(s) 172, 248, 622, 1036
Bme1390I CCNGG 1 cut(s) 300
BmeRI GACNNNNNGTC 1 cut(s) 990
BmeT110I CYCGRG 1 cut(s) 237
BmiI GGNNCC 3 cut(s) 19, 297, 660
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 8 cut(s) 339, 370, 436, 466, 540, 817, 921, 943
BpiI GAAGAC 1 cut(s) 840
Bpu1102I GCTNAGC 1 cut(s) 1007
BsaAI YACGTR 1 cut(s) 653
BsaJI CCNNGG 3 cut(s) 299, 727, 872
BsaXI ACNNNNNCTCC 2 cut(s) 82, 112
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 823
Bse1I ACTGG 2 cut(s) 220, 928
BseBI CCWGG 1 cut(s) 300
BseDI CCNNGG 3 cut(s) 299, 727, 872
BseGI GGATG 4 cut(s) 330, 499, 531, 832
BseLI CCNNNNNNNGG 2 cut(s) 107, 823
BseMII CTCAG 3 cut(s) 32, 621, 998
BseNI ACTGG 2 cut(s) 220, 928
BseSI GKGCMC 1 cut(s) 20
BseX3I CGGCCG 1 cut(s) 168
BseXI GCAGC 3 cut(s) 233, 607, 1046
BseYI CCCAGC 2 cut(s) 527, 549
Bsh1236I CGCG 1 cut(s) 504
Bsh1285I CGRYCG 1 cut(s) 171
BshFI GGCC 2 cut(s) 170, 838
BshNI GGYRCC 1 cut(s) 17
BsiEI CGRYCG 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 409
BsiHKCI CYCGRG 1 cut(s) 237
BsiSI CCGG 1 cut(s) 101
BslI CCNNNNNNNGG 2 cut(s) 107, 823
BsmAI GTCTC 2 cut(s) 631, 977
BsmI GAATGC 1 cut(s) 190
BsnI GGCC 2 cut(s) 170, 838
BsoBI CYCGRG 1 cut(s) 237
Bsp1286I GDGCHC 2 cut(s) 20, 409
Bsp143I GATC 5 cut(s) 496, 537, 658, 841, 943
Bsp1720I GCTNAGC 1 cut(s) 1007
Bsp68I TCGCGA 1 cut(s) 504
BspACI CCGC 4 cut(s) 171, 275, 449, 605
BspANI GGCC 2 cut(s) 170, 838
BspCNI CTCAG 3 cut(s) 33, 622, 999
BspFNI CGCG 1 cut(s) 504
BspHI TCATGA 1 cut(s) 946
BspLI GGNNCC 3 cut(s) 19, 297, 660
BspMAI CTGCAG 1 cut(s) 625
BspPI GGATC 3 cut(s) 653, 666, 951
BspT107I GGYRCC 1 cut(s) 17
BsrI ACTGG 2 cut(s) 220, 928
BssECI CCNNGG 3 cut(s) 299, 727, 872
BssMI GATC 5 cut(s) 496, 537, 658, 841, 943
BssT1I CCWWGG 2 cut(s) 727, 872
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 2 cut(s) 404, 1046
BstBAI YACGTR 1 cut(s) 653
BstC8I GCNNGC 3 cut(s) 68, 175, 471
BstDEI CTNAG 3 cut(s) 41, 630, 1007
BstF5I GGATG 4 cut(s) 330, 499, 531, 832
BstFNI CGCG 1 cut(s) 504
BstKTI GATC 5 cut(s) 499, 540, 661, 844, 946
BstMAI GTCTC 2 cut(s) 631, 977
BstMBI GATC 5 cut(s) 496, 537, 658, 841, 943
BstMCI CGRYCG 1 cut(s) 171
BstMWI GCNNNNNNNGC 3 cut(s) 24, 611, 620
BstNI CCWGG 1 cut(s) 300
BstNSI RCATGY 2 cut(s) 273, 473
BstSCI CCNGG 1 cut(s) 298
BstSFI CTRYAG 2 cut(s) 79, 621
BstSLI GKGCMC 1 cut(s) 20
BstUI CGCG 1 cut(s) 504
BstV1I GCAGC 3 cut(s) 233, 607, 1046
BstV2I GAAGAC 1 cut(s) 840
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
BstZI CGGCCG 1 cut(s) 168
BsuI GTATCC 1 cut(s) 589
BsuRI GGCC 2 cut(s) 170, 838
BtsCI GGATG 4 cut(s) 330, 499, 531, 832
BtsIMutI CAGTG 2 cut(s) 409, 921
BtuMI TCGCGA 1 cut(s) 504
Cac8I GCNNGC 3 cut(s) 68, 175, 471
CaiI CAGNNNCTG 1 cut(s) 629
CciI TCATGA 1 cut(s) 946
Csp6I GTAC 6 cut(s) 213, 219, 671, 890, 1001, 1028
CviAII CATG 4 cut(s) 270, 470, 707, 947
CviQI GTAC 6 cut(s) 213, 219, 671, 890, 1001, 1028
DdeI CTNAG 3 cut(s) 41, 630, 1007
DpnI GATC 5 cut(s) 498, 539, 660, 843, 945
DpnII GATC 5 cut(s) 496, 537, 658, 841, 943
DraI TTTAAA 1 cut(s) 264
DraIII CACNNNGTG 1 cut(s) 185
DriI GACNNNNNGTC 1 cut(s) 990
EaeI YGGCCR 2 cut(s) 168, 836
EagI CGGCCG 1 cut(s) 168
Eam1105I GACNNNNNGTC 1 cut(s) 990
EclXI CGGCCG 1 cut(s) 168
Eco130I CCWWGG 2 cut(s) 727, 872
Eco52I CGGCCG 1 cut(s) 168
Eco57I CTGAAG 2 cut(s) 106, 801
Eco88I CYCGRG 1 cut(s) 237
EcoRI GAATTC 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 298
EcoT14I CCWWGG 2 cut(s) 727, 872
EcoT22I ATGCAT 1 cut(s) 936
ErhI CCWWGG 2 cut(s) 727, 872
FaeI CATG 4 cut(s) 273, 473, 710, 950
FatI CATG 4 cut(s) 269, 469, 706, 946
Fnu4HI GCNGC 4 cut(s) 171, 247, 621, 1035
FokI GGATG 4 cut(s) 317, 506, 518, 839
Fsp4HI GCNGC 4 cut(s) 171, 247, 621, 1035
FspBI CTAG 2 cut(s) 222, 348
GluI GCNGC 4 cut(s) 171, 247, 621, 1035
GsaI CCCAGC 2 cut(s) 531, 553
HaeIII GGCC 2 cut(s) 170, 838
HapII CCGG 1 cut(s) 101
Hin1II CATG 4 cut(s) 273, 473, 710, 950
HinfI GANTC 4 cut(s) 240, 577, 820, 867
HpaII CCGG 1 cut(s) 101
HphI GGTGA 2 cut(s) 171, 967
Hpy166II GTNNAC 6 cut(s) 420, 687, 812, 921, 958, 1028
Hpy188I TCNGA 7 cut(s) 42, 125, 160, 196, 631, 801, 939
Hpy188III TCNNGA 8 cut(s) 357, 491, 503, 535, 817, 824, 947, 995
Hpy8I GTNNAC 6 cut(s) 420, 687, 812, 921, 958, 1028
HpyAV CCTTC 5 cut(s) 39, 45, 130, 221, 973
HpyCH4III ACNGT 2 cut(s) 404, 1046
HpyCH4IV ACGT 3 cut(s) 652, 765, 999
HpyCH4V TGCA 5 cut(s) 53, 479, 623, 637, 934
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 611, 620
HpyF3I CTNAG 3 cut(s) 41, 630, 1007
HpySE526I ACGT 3 cut(s) 652, 765, 999
Hsp92II CATG 4 cut(s) 273, 473, 710, 950
Kzo9I GATC 5 cut(s) 496, 537, 658, 841, 943
LmnI GCTCC 1 cut(s) 103
Lsp1109I GCAGC 3 cut(s) 233, 607, 1046
LweI GCATC 8 cut(s) 339, 370, 436, 466, 540, 817, 921, 943
MaeI CTAG 2 cut(s) 222, 348
MaeII ACGT 3 cut(s) 652, 765, 999
MaeIII GTNAC 2 cut(s) 761, 770
MalI GATC 5 cut(s) 498, 539, 660, 843, 945
MboI GATC 5 cut(s) 496, 537, 658, 841, 943
MboII GAAGA 4 cut(s) 491, 794, 840, 843
MfeI CAATTG 2 cut(s) 441, 480
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 2 cut(s) 20, 409
MluCI AATT 7 cut(s) 154, 231, 361, 441, 480, 638, 974
MlyI GAGTC 3 cut(s) 249, 571, 861
MmeI TCCRAC 3 cut(s) 113, 547, 925
Mph1103I ATGCAT 1 cut(s) 936
MroXI GAANNNNTTC 1 cut(s) 231
MseI TTAA 3 cut(s) 263, 642, 1048
MslI CAYNNNNRTG 3 cut(s) 468, 474, 926
MspA1I CMGCKG 3 cut(s) 173, 246, 1006
MspI CCGG 1 cut(s) 101
MspR9I CCNGG 1 cut(s) 300
MunI CAATTG 2 cut(s) 441, 480
Mva1269I GAATGC 1 cut(s) 190
MvaI CCWGG 1 cut(s) 300
MvnI CGCG 1 cut(s) 504
MwoI GCNNNNNNNGC 3 cut(s) 24, 611, 620
NdeII GATC 5 cut(s) 496, 537, 658, 841, 943
NlaIII CATG 4 cut(s) 273, 473, 710, 950
NlaIV GGNNCC 3 cut(s) 19, 297, 660
NmuCI GTSAC 2 cut(s) 761, 770
NruI TCGCGA 1 cut(s) 504
NsiI ATGCAT 1 cut(s) 936
NspI RCATGY 2 cut(s) 273, 473
PaeI GCATGC 1 cut(s) 473
PaeR7I CTCGAG 1 cut(s) 237
PagI TCATGA 1 cut(s) 946
PciI ACATGT 1 cut(s) 269
PctI GAATGC 1 cut(s) 190
PdmI GAANNNNTTC 1 cut(s) 231
PfeI GAWTC 1 cut(s) 820
PkrI GCNGC 4 cut(s) 172, 248, 622, 1036
PleI GAGTC 3 cut(s) 248, 571, 861
PpsI GAGTC 3 cut(s) 248, 571, 861
Ppu21I YACGTR 1 cut(s) 653
PscI ACATGT 1 cut(s) 269
PsiI TTATAA 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 298
PspFI CCCAGC 2 cut(s) 527, 549
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 3 cut(s) 19, 297, 660
PspXI VCTCGAGB 1 cut(s) 237
PstI CTGCAG 1 cut(s) 625
PstNI CAGNNNCTG 1 cut(s) 629
PsuI RGATCY 1 cut(s) 658
PvuII CAGCTG 2 cut(s) 246, 1006
RruI TCGCGA 1 cut(s) 504
RsaI GTAC 6 cut(s) 214, 220, 672, 891, 1002, 1029
RsaNI GTAC 6 cut(s) 213, 219, 671, 890, 1001, 1028
RseI CAYNNNNRTG 3 cut(s) 468, 474, 926
SaqAI TTAA 3 cut(s) 263, 642, 1048
SatI GCNGC 4 cut(s) 171, 247, 621, 1035
Sau3AI GATC 5 cut(s) 496, 537, 658, 841, 943
SchI GAGTC 3 cut(s) 249, 571, 861
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 2 cut(s) 20, 409
SfaNI GCATC 8 cut(s) 339, 370, 436, 466, 540, 817, 921, 943
SfcI CTRYAG 2 cut(s) 79, 621
Sfr274I CTCGAG 1 cut(s) 237
SlaI CTCGAG 1 cut(s) 237
SmiMI CAYNNNNRTG 3 cut(s) 468, 474, 926
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
SpeI ACTAGT 1 cut(s) 221
SphI GCATGC 1 cut(s) 473
Sse9I AATT 7 cut(s) 154, 231, 361, 441, 480, 638, 974
SsiI CCGC 4 cut(s) 171, 275, 449, 605
SspMI CTAG 2 cut(s) 222, 348
StyD4I CCNGG 1 cut(s) 298
StyI CCWWGG 2 cut(s) 727, 872
TaaI ACNGT 2 cut(s) 404, 1046
TaiI ACGT 3 cut(s) 655, 768, 1002
TaqI TCGA 1 cut(s) 238
TaqII GACCGA 1 cut(s) 834
TasI AATT 7 cut(s) 154, 231, 361, 441, 480, 638, 974
TatI WGTACW 1 cut(s) 670
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 1 cut(s) 820
Tru1I TTAA 3 cut(s) 263, 642, 1048
Tru9I TTAA 3 cut(s) 263, 642, 1048
TscAI CASTG 2 cut(s) 409, 928
TseFI GTSAC 2 cut(s) 761, 770
TseI GCWGC 3 cut(s) 246, 620, 1034
Tsp45I GTSAC 2 cut(s) 761, 770
TspDTI ATGAA 2 cut(s) 584, 963
TspGWI ACGGA 2 cut(s) 907, 1032
TspRI CASTG 2 cut(s) 409, 928
XapI RAATTY 1 cut(s) 231
XceI RCATGY 2 cut(s) 273, 473
XhoI CTCGAG 1 cut(s) 237
XmnI GAANNNNTTC 1 cut(s) 231
XspI CTAG 2 cut(s) 222, 348
Zsp2I ATGCAT 1 cut(s) 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.