pycom09g10880

Hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0005743
Physical Location & Seq
Forward (+)
8969373 .. 8973326
3954 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g10880.10

Sequence Viewer

Length: 873 bp
ATGGTTCAACTTTTGGGCACCATTCGCCCTTCTCCTTCTCTGAGCTACTTTGCAACATACAAACGCCAGCTCTCACTCCACAGCCCTCCAACCAAAGCTCCGGCAAATGGGTTTTCTCCCTTCAGACGAGTTTTCAACTCTTACCTCCAATCTAATTCAGAAACTACGGCCGCTGGCTCACCTTGTGAGCATTCAGAAATCATATTTATAGGTACCGGTACTAGTGAAGGAATTCCCCGAGTCAGCTGCCTCACAAATCCTTTAAAGACATGTGCGGTTTGCTTGAAAGCTGTGGAACCAGGGAATAAAAATAAGAGGCTCAACACAAGCATCCTTGTTCGTTATCCTAGACCCTCTGGAAATTATAACATTCTTATAGATGCTGGAAAGTTTTTCTACCACAGTGCTCTCCAATGGTTTACTGCCTATGGGATAAGAACAATTGATGCGGTTATTATTACTCATTCGCATGCTGATGCAATTGGAGGTCTGGATGATCTTCGCGATTGGACAAACAATGTCCAACCCAGCATCCCGATCTATGTTGCCCAGCGTGATTTTGAGGTAATGAAAAAGACTCATTATTATTTGGTGGATACAAGTGCGGTTCTTGCTGGTGCTGCAGTCTCTGAGTTGCAATTTAATATCATACTGGAGGATCCATTTGTAGTACAAGATTTGAAGTTTACCCCTTTACCTGTGTGGCATGGTCAGGGTTATCGTTCCCTTGGATTTCGTTTTGGTAATGATGCTTTACGGCCAGATCGGTCTTCTTCAACGCATTTTGGACTCCCAAGGGCTTTGGAGGAAGTACGGAAAATCCAACCGAGAAAGACTTTGTTCACTGGTATGATGCATCTGATGGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lactamase_B Lactamase_B_2

Protein Analysis

291

Amino Acids

32.42

Weight (kDa)

9.35

Isoelectric Point (pI)

51.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 366
Acc65I GGTACC 1 cut(s) 212
AccB1I GGYRCC 2 cut(s) 17, 212
AccII CGCG 1 cut(s) 504
AciI CCGC 4 cut(s) 171, 275, 449, 605
AclWI GGATC 2 cut(s) 653, 666
AcoI YGGCCR 2 cut(s) 168, 758
AcsI RAATTY 1 cut(s) 231
AcuI CTGAAG 1 cut(s) 106
AdeI CACNNNGTG 1 cut(s) 185
AfaI GTAC 4 cut(s) 214, 220, 672, 813
AfiI CCNNNNNNNGG 1 cut(s) 107
AflIII ACRYGT 1 cut(s) 269
AgeI ACCGGT 1 cut(s) 215
AgsI TTSAA 5 cut(s) 8, 136, 286, 682, 777
AhlI ACTAGT 1 cut(s) 221
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 5 cut(s) 45, 70, 98, 246, 290
AluI AGCT 5 cut(s) 45, 70, 98, 246, 290
Alw21I GWGCWC 1 cut(s) 409
Alw26I GTCTC 1 cut(s) 631
AlwI GGATC 2 cut(s) 653, 666
AlwNI CAGNNNCTG 1 cut(s) 629
Ama87I CYCGRG 1 cut(s) 237
AoxI GGCC 2 cut(s) 168, 758
ApeKI GCWGC 2 cut(s) 246, 620
ApoI RAATTY 1 cut(s) 231
AsiGI ACCGGT 1 cut(s) 215
Asp700I GAANNNNTTC 1 cut(s) 231
Asp718I GGTACC 1 cut(s) 212
AsuHPI GGTGA 1 cut(s) 171
AvaI CYCGRG 1 cut(s) 237
BaeGI GKGCMC 1 cut(s) 20
BamHI GGATCC 1 cut(s) 658
BanI GGYRCC 2 cut(s) 17, 212
BbsI GAAGAC 1 cut(s) 762
Bbv12I GWGCWC 1 cut(s) 409
BbvI GCAGC 2 cut(s) 233, 607
BccI CCATC 1 cut(s) 856
BceAI ACGGC 2 cut(s) 183, 773
BcgI CGANNNNNNTGC 2 cut(s) 228, 262
BciT130I CCWGG 1 cut(s) 300
BciVI GTATCC 1 cut(s) 589
BcoDI GTCTC 1 cut(s) 631
BcuI ACTAGT 1 cut(s) 221
BfaI CTAG 2 cut(s) 222, 348
BfmI CTRYAG 1 cut(s) 621
BfuI GTATCC 1 cut(s) 589
BisI GCNGC 3 cut(s) 171, 247, 621
BlsI GCNGC 3 cut(s) 172, 248, 622
Bme1390I CCNGG 1 cut(s) 300
BmeT110I CYCGRG 1 cut(s) 237
BmiI GGNNCC 4 cut(s) 19, 214, 297, 660
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 8 cut(s) 339, 370, 436, 466, 540, 739, 843, 865
BpiI GAAGAC 1 cut(s) 762
BpmI CTGGAG 1 cut(s) 674
Bsa29I ATCGAT 1 cut(s) 868
BsaJI CCNNGG 3 cut(s) 299, 727, 794
BsaWI WCCGGW 1 cut(s) 215
BsaXI ACNNNNNCTCC 2 cut(s) 82, 112
Bsc4I CCNNNNNNNGG 1 cut(s) 107
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 2 cut(s) 657, 850
BseBI CCWGG 1 cut(s) 300
BseCI ATCGAT 1 cut(s) 868
BseDI CCNNGG 3 cut(s) 299, 727, 794
BseGI GGATG 3 cut(s) 330, 499, 531
BseLI CCNNNNNNNGG 1 cut(s) 107
BseMII CTCAG 2 cut(s) 32, 621
BseNI ACTGG 2 cut(s) 657, 850
BseSI GKGCMC 1 cut(s) 20
BseX3I CGGCCG 1 cut(s) 168
BseXI GCAGC 2 cut(s) 233, 607
BseYI CCCAGC 2 cut(s) 527, 549
Bsh1236I CGCG 1 cut(s) 504
Bsh1285I CGRYCG 1 cut(s) 171
BshFI GGCC 2 cut(s) 170, 760
BshNI GGYRCC 2 cut(s) 17, 212
BshTI ACCGGT 1 cut(s) 215
BshVI ATCGAT 1 cut(s) 868
BsiEI CGRYCG 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 409
BsiHKCI CYCGRG 1 cut(s) 237
BsiSI CCGG 2 cut(s) 101, 216
BslI CCNNNNNNNGG 1 cut(s) 107
BsmAI GTCTC 1 cut(s) 631
BsmI GAATGC 1 cut(s) 190
BsnI GGCC 2 cut(s) 170, 760
BsoBI CYCGRG 1 cut(s) 237
Bsp1286I GDGCHC 2 cut(s) 20, 409
Bsp143I GATC 5 cut(s) 496, 537, 658, 763, 865
Bsp68I TCGCGA 1 cut(s) 504
BspACI CCGC 4 cut(s) 171, 275, 449, 605
BspANI GGCC 2 cut(s) 170, 760
BspCNI CTCAG 2 cut(s) 33, 622
BspDI ATCGAT 1 cut(s) 868
BspFNI CGCG 1 cut(s) 504
BspLI GGNNCC 4 cut(s) 19, 214, 297, 660
BspMAI CTGCAG 1 cut(s) 625
BspPI GGATC 2 cut(s) 653, 666
BspT107I GGYRCC 2 cut(s) 17, 212
BsrFI RCCGGY 1 cut(s) 215
BsrI ACTGG 2 cut(s) 657, 850
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 3 cut(s) 299, 727, 794
BssMI GATC 5 cut(s) 496, 537, 658, 763, 865
BssT1I CCWWGG 2 cut(s) 727, 794
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 1 cut(s) 404
BstC8I GCNNGC 3 cut(s) 68, 175, 471
BstDEI CTNAG 2 cut(s) 41, 630
BstF5I GGATG 3 cut(s) 330, 499, 531
BstFNI CGCG 1 cut(s) 504
BstKTI GATC 5 cut(s) 499, 540, 661, 766, 868
BstMAI GTCTC 1 cut(s) 631
BstMBI GATC 5 cut(s) 496, 537, 658, 763, 865
BstMCI CGRYCG 1 cut(s) 171
BstMWI GCNNNNNNNGC 3 cut(s) 24, 611, 620
BstNI CCWGG 1 cut(s) 300
BstNSI RCATGY 2 cut(s) 273, 473
BstSCI CCNGG 1 cut(s) 298
BstSFI CTRYAG 1 cut(s) 621
BstSLI GKGCMC 1 cut(s) 20
BstUI CGCG 1 cut(s) 504
BstV1I GCAGC 2 cut(s) 233, 607
BstV2I GAAGAC 1 cut(s) 762
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
BstZI CGGCCG 1 cut(s) 168
Bsu15I ATCGAT 1 cut(s) 868
BsuI GTATCC 1 cut(s) 589
BsuRI GGCC 2 cut(s) 170, 760
BsuTUI ATCGAT 1 cut(s) 868
BtsCI GGATG 3 cut(s) 330, 499, 531
BtsIMutI CAGTG 2 cut(s) 409, 843
BtuMI TCGCGA 1 cut(s) 504
Cac8I GCNNGC 3 cut(s) 68, 175, 471
CaiI CAGNNNCTG 1 cut(s) 629
Cfr10I RCCGGY 1 cut(s) 215
ClaI ATCGAT 1 cut(s) 868
Csp6I GTAC 4 cut(s) 213, 219, 671, 812
CspAI ACCGGT 1 cut(s) 215
CviAII CATG 3 cut(s) 270, 470, 707
CviQI GTAC 4 cut(s) 213, 219, 671, 812
DdeI CTNAG 2 cut(s) 41, 630
DpnI GATC 5 cut(s) 498, 539, 660, 765, 867
DpnII GATC 5 cut(s) 496, 537, 658, 763, 865
DraI TTTAAA 1 cut(s) 264
DraIII CACNNNGTG 1 cut(s) 185
EaeI YGGCCR 2 cut(s) 168, 758
EagI CGGCCG 1 cut(s) 168
EclXI CGGCCG 1 cut(s) 168
Eco130I CCWWGG 2 cut(s) 727, 794
Eco52I CGGCCG 1 cut(s) 168
Eco57I CTGAAG 1 cut(s) 106
Eco88I CYCGRG 1 cut(s) 237
EcoRI GAATTC 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 298
EcoT14I CCWWGG 2 cut(s) 727, 794
EcoT22I ATGCAT 1 cut(s) 858
ErhI CCWWGG 2 cut(s) 727, 794
FaeI CATG 3 cut(s) 273, 473, 710
FatI CATG 3 cut(s) 269, 469, 706
Fnu4HI GCNGC 3 cut(s) 171, 247, 621
FokI GGATG 3 cut(s) 317, 506, 518
Fsp4HI GCNGC 3 cut(s) 171, 247, 621
FspBI CTAG 2 cut(s) 222, 348
GluI GCNGC 3 cut(s) 171, 247, 621
GsaI CCCAGC 2 cut(s) 531, 553
GsuI CTGGAG 1 cut(s) 674
HaeIII GGCC 2 cut(s) 170, 760
HapII CCGG 2 cut(s) 101, 216
Hin1II CATG 3 cut(s) 273, 473, 710
HinfI GANTC 3 cut(s) 240, 577, 789
HpaII CCGG 2 cut(s) 101, 216
HphI GGTGA 1 cut(s) 171
Hpy166II GTNNAC 3 cut(s) 420, 687, 843
Hpy188I TCNGA 6 cut(s) 42, 125, 160, 196, 631, 861
Hpy188III TCNNGA 4 cut(s) 357, 491, 503, 535
Hpy8I GTNNAC 3 cut(s) 420, 687, 843
HpyAV CCTTC 4 cut(s) 39, 45, 130, 221
HpyCH4III ACNGT 1 cut(s) 404
HpyCH4V TGCA 5 cut(s) 53, 479, 623, 637, 856
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 611, 620
HpyF3I CTNAG 2 cut(s) 41, 630
Hsp92II CATG 3 cut(s) 273, 473, 710
KpnI GGTACC 1 cut(s) 216
Kzo9I GATC 5 cut(s) 496, 537, 658, 763, 865
LmnI GCTCC 1 cut(s) 103
Lsp1109I GCAGC 2 cut(s) 233, 607
LweI GCATC 8 cut(s) 339, 370, 436, 466, 540, 739, 843, 865
MaeI CTAG 2 cut(s) 222, 348
MalI GATC 5 cut(s) 498, 539, 660, 765, 867
MboI GATC 5 cut(s) 496, 537, 658, 763, 865
MboII GAAGA 3 cut(s) 491, 762, 765
MfeI CAATTG 2 cut(s) 441, 480
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 2 cut(s) 20, 409
MluCI AATT 6 cut(s) 154, 231, 361, 441, 480, 638
MlyI GAGTC 3 cut(s) 249, 571, 783
MmeI TCCRAC 3 cut(s) 113, 547, 847
MnlI CCTC 9 cut(s) 96, 155, 260, 309, 364, 479, 556, 649, 799
Mph1103I ATGCAT 1 cut(s) 858
MroXI GAANNNNTTC 1 cut(s) 231
MseI TTAA 2 cut(s) 263, 642
MslI CAYNNNNRTG 3 cut(s) 468, 474, 848
MspA1I CMGCKG 2 cut(s) 173, 246
MspI CCGG 2 cut(s) 101, 216
MspR9I CCNGG 1 cut(s) 300
MunI CAATTG 2 cut(s) 441, 480
Mva1269I GAATGC 1 cut(s) 190
MvaI CCWGG 1 cut(s) 300
MvnI CGCG 1 cut(s) 504
MwoI GCNNNNNNNGC 3 cut(s) 24, 611, 620
NdeII GATC 5 cut(s) 496, 537, 658, 763, 865
NlaIII CATG 3 cut(s) 273, 473, 710
NlaIV GGNNCC 4 cut(s) 19, 214, 297, 660
NruI TCGCGA 1 cut(s) 504
NsiI ATGCAT 1 cut(s) 858
NspI RCATGY 2 cut(s) 273, 473
PaeI GCATGC 1 cut(s) 473
PciI ACATGT 1 cut(s) 269
PctI GAATGC 1 cut(s) 190
PdmI GAANNNNTTC 1 cut(s) 231
PinAI ACCGGT 1 cut(s) 215
PkrI GCNGC 3 cut(s) 172, 248, 622
PleI GAGTC 3 cut(s) 248, 571, 783
PpsI GAGTC 3 cut(s) 248, 571, 783
PscI ACATGT 1 cut(s) 269
PsiI TTATAA 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 298
PspFI CCCAGC 2 cut(s) 527, 549
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 4 cut(s) 19, 214, 297, 660
PstI CTGCAG 1 cut(s) 625
PstNI CAGNNNCTG 1 cut(s) 629
PsuI RGATCY 1 cut(s) 658
PvuII CAGCTG 1 cut(s) 246
RruI TCGCGA 1 cut(s) 504
RsaI GTAC 4 cut(s) 214, 220, 672, 813
RsaNI GTAC 4 cut(s) 213, 219, 671, 812
RseI CAYNNNNRTG 3 cut(s) 468, 474, 848
SaqAI TTAA 2 cut(s) 263, 642
SatI GCNGC 3 cut(s) 171, 247, 621
Sau3AI GATC 5 cut(s) 496, 537, 658, 763, 865
SchI GAGTC 3 cut(s) 249, 571, 783
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 2 cut(s) 20, 409
SfaNI GCATC 8 cut(s) 339, 370, 436, 466, 540, 739, 843, 865
SfcI CTRYAG 1 cut(s) 621
SmiMI CAYNNNNRTG 3 cut(s) 468, 474, 848
SpeI ACTAGT 1 cut(s) 221
SphI GCATGC 1 cut(s) 473
Sse9I AATT 6 cut(s) 154, 231, 361, 441, 480, 638
SsiI CCGC 4 cut(s) 171, 275, 449, 605
SspMI CTAG 2 cut(s) 222, 348
StyD4I CCNGG 1 cut(s) 298
StyI CCWWGG 2 cut(s) 727, 794
TaaI ACNGT 1 cut(s) 404
TaqI TCGA 1 cut(s) 868
TaqII GACCGA 1 cut(s) 756
TasI AATT 6 cut(s) 154, 231, 361, 441, 480, 638
TatI WGTACW 1 cut(s) 670
TauI GCSGC 1 cut(s) 173
Tru1I TTAA 2 cut(s) 263, 642
Tru9I TTAA 2 cut(s) 263, 642
TscAI CASTG 2 cut(s) 409, 850
TseI GCWGC 2 cut(s) 246, 620
TspDTI ATGAA 1 cut(s) 584
TspGWI ACGGA 1 cut(s) 829
TspRI CASTG 2 cut(s) 409, 850
XapI RAATTY 1 cut(s) 231
XceI RCATGY 2 cut(s) 273, 473
XmnI GAANNNNTTC 1 cut(s) 231
XspI CTAG 2 cut(s) 222, 348
Zsp2I ATGCAT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.