Prupe.3G048500_v2.0.a1

Hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0005743
Physical Location & Seq
Forward (+)
3418940 .. 3423799
4860 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G048500.1

Sequence Viewer

Length: 1050 bp
ATGGTTCAATGTCTGGGCACCATCCGGCCCTCTCCTTCTCTAAGCTATTTTGCTACTTACAAACGCCAACTATCATTCCGTAGCTCCCCCATCACAGGTCCTGCAAATGGGTTCTCTCCCTTCAGACGAATTTTCAATGCTTACCTTCAATCTAATTCAGCAACCACCGGCGCTGGCTCACAGTCACCTTCTGAACCGTCGGAAGTCATATTTATAGGTACAGGTACTAGTGAAGGAATTCCACGCGTGAGCTGCCTGACACATCCTTTAAAGACATGTGAGGTTTGCTCGAAAGCTGCGGAACCAGGTAATAAAAATCGGAGACTCAATACAAGCATCCTTGTTCGTTATCCTAGGCCATCTGGAAGTTGTAATGTTCTTATTGATGCTGGAAAGTTTTTCTACCACAGTGCTCTAAAATGGTTTCCTTCCTATGGGATAAGAACAATTGATGCGGTTATTATTACTCATTCTCATGCTGATGCAATTGGAGGTCTGGATGACCTTCGTGATTGGACAAACAATGTTCAACCCCACATTCCTATATATGTGGCCCAGCGTGATTTTGAGGTGATGAAAAAGACTCATTATTATTTGGTGGATACAAGTTTCGTCCTTGCTGGTGCTGCTGTCTCAGAGTTGCAATTTAATATCATACACGAGGATCCATTTTCAGTACAAGGGTTGCAGTTTACCCCTTTACCTGTGTGGCATGGTCGTGGTTATCGTTCCCTTGGATTTCGTTTTGGTAATGTTTGTTATATTAGTGACGTCAGTGACATACCTGAAGAAACTTATCCACTTCTGAAAAACTGTGAACTTCTGATTCTGGATGCTTTGCGGCCAGATCGGTCTACTGCAACACATTTTGGACTTCCAAGGGCTTTGGAGGAAGTACGGAAAATCCAACCGAAGAGAACTTTGTTCACTGGTATGATGCATCTGATGGATCATGAAAAGGTGAGTGATTATCTTATGAAATTGATGGAGACAGAGGGTCTTGATGTACAACTGAGCTATGATGGACTTCGTGTACCAGTAGCATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lactamase_B Lactamase_B_2

Protein Analysis

350

Amino Acids

39.05

Weight (kDa)

8.11

Isoelectric Point (pI)

53.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 774
AccB1I GGYRCC 1 cut(s) 17
AccI GTMKAC 1 cut(s) 854
AccII CGCG 1 cut(s) 246
AciI CCGC 3 cut(s) 299, 455, 841
AclWI GGATC 3 cut(s) 659, 672, 957
AcoI YGGCCR 1 cut(s) 842
AcsI RAATTY 2 cut(s) 129, 237
AcuI CTGAAG 2 cut(s) 106, 807
AcyI GRCGYC 1 cut(s) 771
AfaI GTAC 6 cut(s) 220, 226, 678, 897, 1008, 1035
AfiI CCNNNNNNNGG 3 cut(s) 95, 107, 434
AflIII ACRYGT 2 cut(s) 244, 275
AgsI TTSAA 4 cut(s) 8, 136, 149, 530
AhdI GACNNNNNGTC 1 cut(s) 996
AhlI ACTAGT 1 cut(s) 227
AjnI CCWGG 1 cut(s) 304
AluBI AGCT 5 cut(s) 45, 84, 252, 296, 1017
AluI AGCT 5 cut(s) 45, 84, 252, 296, 1017
Alw21I GWGCWC 1 cut(s) 415
Alw26I GTCTC 3 cut(s) 316, 637, 983
AlwI GGATC 3 cut(s) 659, 672, 957
AlwNI CAGNNNCTG 1 cut(s) 101
AoxI GGCC 4 cut(s) 26, 356, 552, 842
ApeKI GCWGC 3 cut(s) 252, 296, 626
ApoI RAATTY 2 cut(s) 129, 237
Asp700I GAANNNNTTC 1 cut(s) 237
AspA2I CCTAGG 1 cut(s) 353
AspLEI GCGC 1 cut(s) 173
AspS9I GGNCC 3 cut(s) 27, 98, 553
AsuHPI GGTGA 3 cut(s) 177, 583, 973
AvaII GGWCC 1 cut(s) 98
AvrII CCTAGG 1 cut(s) 353
BaeGI GKGCMC 1 cut(s) 20
BamHI GGATCC 1 cut(s) 664
BanI GGYRCC 1 cut(s) 17
BauI CACGAG 1 cut(s) 659
Bbv12I GWGCWC 1 cut(s) 415
BbvI GCAGC 3 cut(s) 239, 283, 613
BccI CCATC 6 cut(s) 29, 98, 367, 940, 979, 1016
BciT130I CCWGG 1 cut(s) 306
BciVI GTATCC 1 cut(s) 595
BcoDI GTCTC 3 cut(s) 316, 637, 983
BcuI ACTAGT 1 cut(s) 227
BfaI CTAG 3 cut(s) 228, 354, 1048
BfoI RGCGCY 1 cut(s) 174
BfuI GTATCC 1 cut(s) 595
BisI GCNGC 4 cut(s) 253, 297, 627, 842
BlnI CCTAGG 1 cut(s) 353
BlsI GCNGC 4 cut(s) 254, 298, 628, 843
Bme1390I CCNGG 1 cut(s) 306
Bme18I GGWCC 1 cut(s) 98
BmeRI GACNNNNNGTC 1 cut(s) 996
BmgT120I GGNCC 3 cut(s) 27, 98, 553
BmiI GGNNCC 3 cut(s) 19, 303, 666
BmrFI CCNGG 1 cut(s) 306
BmsI GCATC 7 cut(s) 345, 376, 442, 472, 823, 927, 949
BplI GAGNNNNNCTC 2 cut(s) 272, 304
BsaHI GRCGYC 1 cut(s) 771
BsaJI CCNNGG 3 cut(s) 353, 733, 878
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 107, 434
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 2 cut(s) 934, 1037
BseBI CCWGG 1 cut(s) 306
BseDI CCNNGG 3 cut(s) 353, 733, 878
BseGI GGATG 5 cut(s) 21, 262, 336, 505, 838
BseLI CCNNNNNNNGG 3 cut(s) 95, 107, 434
BseMII CTCAG 2 cut(s) 648, 1004
BseNI ACTGG 2 cut(s) 934, 1037
BseSI GKGCMC 1 cut(s) 20
BseXI GCAGC 3 cut(s) 239, 283, 613
BseYI CCCAGC 1 cut(s) 555
Bsh1236I CGCG 1 cut(s) 246
BshFI GGCC 4 cut(s) 28, 358, 554, 844
BshNI GGYRCC 1 cut(s) 17
BsiHKAI GWGCWC 1 cut(s) 415
BsiSI CCGG 2 cut(s) 25, 168
BslI CCNNNNNNNGG 3 cut(s) 95, 107, 434
BsmAI GTCTC 3 cut(s) 316, 637, 983
BsmI GAATGC 1 cut(s) 1043
BsnI GGCC 4 cut(s) 28, 358, 554, 844
Bsp1286I GDGCHC 2 cut(s) 20, 415
Bsp1407I TGTACA 1 cut(s) 1006
Bsp143I GATC 3 cut(s) 664, 847, 949
BspACI CCGC 3 cut(s) 299, 455, 841
BspANI GGCC 4 cut(s) 28, 358, 554, 844
BspCNI CTCAG 2 cut(s) 647, 1005
BspFNI CGCG 1 cut(s) 246
BspHI TCATGA 1 cut(s) 952
BspLI GGNNCC 3 cut(s) 19, 303, 666
BspPI GGATC 3 cut(s) 659, 672, 957
BspT107I GGYRCC 1 cut(s) 17
BsrFI RCCGGY 1 cut(s) 167
BsrGI TGTACA 1 cut(s) 1006
BsrI ACTGG 2 cut(s) 934, 1037
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 3 cut(s) 353, 733, 878
BssMI GATC 3 cut(s) 664, 847, 949
BssNI GRCGYC 1 cut(s) 771
BssSI CACGAG 1 cut(s) 659
BssT1I CCWWGG 3 cut(s) 353, 733, 878
Bst2BI CACGAG 1 cut(s) 659
Bst2UI CCWGG 1 cut(s) 306
Bst4CI ACNGT 4 cut(s) 183, 198, 410, 815
Bst6I CTCTTC 1 cut(s) 908
BstACI GRCGYC 1 cut(s) 771
BstAUI TGTACA 1 cut(s) 1006
BstC8I GCNNGC 1 cut(s) 175
BstDEI CTNAG 3 cut(s) 41, 634, 1013
BstF5I GGATG 5 cut(s) 21, 262, 336, 505, 838
BstFNI CGCG 1 cut(s) 246
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 1 cut(s) 173
BstKTI GATC 3 cut(s) 667, 850, 952
BstMAI GTCTC 3 cut(s) 316, 637, 983
BstMBI GATC 3 cut(s) 664, 847, 949
BstMWI GCNNNNNNNGC 2 cut(s) 252, 626
BstNI CCWGG 1 cut(s) 306
BstNSI RCATGY 1 cut(s) 279
BstSCI CCNGG 1 cut(s) 304
BstSLI GKGCMC 1 cut(s) 20
BstUI CGCG 1 cut(s) 246
BstV1I GCAGC 3 cut(s) 239, 283, 613
BstX2I RGATCY 1 cut(s) 664
BstYI RGATCY 1 cut(s) 664
BsuI GTATCC 1 cut(s) 595
BsuRI GGCC 4 cut(s) 28, 358, 554, 844
BtsCI GGATG 5 cut(s) 21, 262, 336, 505, 838
BtsIMutI CAGTG 3 cut(s) 415, 781, 927
Cac8I GCNNGC 1 cut(s) 175
CaiI CAGNNNCTG 1 cut(s) 101
CciI TCATGA 1 cut(s) 952
CfoI GCGC 1 cut(s) 173
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 3 cut(s) 27, 98, 553
CsiI ACCWGGT 1 cut(s) 304
Csp6I GTAC 6 cut(s) 219, 225, 677, 896, 1007, 1034
CviAII CATG 4 cut(s) 276, 476, 713, 953
CviQI GTAC 6 cut(s) 219, 225, 677, 896, 1007, 1034
DdeI CTNAG 3 cut(s) 41, 634, 1013
DpnI GATC 3 cut(s) 666, 849, 951
DpnII GATC 3 cut(s) 664, 847, 949
DraI TTTAAA 1 cut(s) 270
DriI GACNNNNNGTC 1 cut(s) 996
EaeI YGGCCR 1 cut(s) 842
Eam1104I CTCTTC 1 cut(s) 908
Eam1105I GACNNNNNGTC 1 cut(s) 996
EarI CTCTTC 1 cut(s) 908
Eco130I CCWWGG 3 cut(s) 353, 733, 878
Eco47I GGWCC 1 cut(s) 98
Eco57I CTGAAG 2 cut(s) 106, 807
EcoO109I RGGNCCY 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 237
EcoRII CCWGG 1 cut(s) 304
EcoT14I CCWWGG 3 cut(s) 353, 733, 878
EcoT22I ATGCAT 1 cut(s) 942
ErhI CCWWGG 3 cut(s) 353, 733, 878
FaeI CATG 4 cut(s) 279, 479, 716, 956
FatI CATG 4 cut(s) 275, 475, 712, 952
FblI GTMKAC 1 cut(s) 854
Fnu4HI GCNGC 4 cut(s) 253, 297, 627, 842
FokI GGATG 5 cut(s) 8, 249, 323, 512, 845
Fsp4HI GCNGC 4 cut(s) 253, 297, 627, 842
FspBI CTAG 3 cut(s) 228, 354, 1048
GlaI GCGC 1 cut(s) 172
GluI GCNGC 4 cut(s) 253, 297, 627, 842
GsaI CCCAGC 1 cut(s) 559
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 4 cut(s) 28, 358, 554, 844
HapII CCGG 2 cut(s) 25, 168
HhaI GCGC 1 cut(s) 173
Hin1I GRCGYC 1 cut(s) 771
Hin1II CATG 4 cut(s) 279, 479, 716, 956
Hin6I GCGC 1 cut(s) 171
HinP1I GCGC 1 cut(s) 171
HinfI GANTC 3 cut(s) 324, 583, 826
HpaII CCGG 2 cut(s) 25, 168
HphI GGTGA 3 cut(s) 177, 583, 973
Hpy166II GTNNAC 5 cut(s) 693, 818, 855, 927, 1034
Hpy188I TCNGA 8 cut(s) 125, 193, 202, 321, 637, 807, 825, 945
Hpy188III TCNNGA 6 cut(s) 363, 497, 509, 830, 953, 1001
Hpy8I GTNNAC 5 cut(s) 693, 818, 855, 927, 1034
Hpy99I CGWCG 1 cut(s) 202
HpyAV CCTTC 7 cut(s) 45, 130, 155, 198, 227, 438, 515
HpyCH4III ACNGT 4 cut(s) 183, 198, 410, 815
HpyCH4IV ACGT 1 cut(s) 771
HpyCH4V TGCA 6 cut(s) 104, 485, 643, 688, 860, 940
HpyF10VI GCNNNNNNNGC 2 cut(s) 252, 626
HpyF3I CTNAG 3 cut(s) 41, 634, 1013
HpySE526I ACGT 1 cut(s) 771
Hsp92I GRCGYC 1 cut(s) 771
Hsp92II CATG 4 cut(s) 279, 479, 716, 956
HspAI GCGC 1 cut(s) 171
Kzo9I GATC 3 cut(s) 664, 847, 949
LmnI GCTCC 1 cut(s) 89
Lsp1109I GCAGC 3 cut(s) 239, 283, 613
LweI GCATC 7 cut(s) 345, 376, 442, 472, 823, 927, 949
MabI ACCWGGT 1 cut(s) 304
MaeI CTAG 3 cut(s) 228, 354, 1048
MaeII ACGT 1 cut(s) 771
MaeIII GTNAC 3 cut(s) 183, 767, 776
MalI GATC 3 cut(s) 666, 849, 951
MboI GATC 3 cut(s) 664, 847, 949
MboII GAAGA 2 cut(s) 800, 925
MfeI CAATTG 2 cut(s) 447, 486
MflI RGATCY 1 cut(s) 664
MhlI GDGCHC 2 cut(s) 20, 415
MluCI AATT 7 cut(s) 129, 154, 237, 447, 486, 644, 980
MluI ACGCGT 1 cut(s) 244
MlyI GAGTC 2 cut(s) 318, 577
MmeI TCCRAC 2 cut(s) 180, 931
MnlI CCTC 7 cut(s) 40, 274, 485, 562, 655, 883, 988
Mph1103I ATGCAT 1 cut(s) 942
MroXI GAANNNNTTC 1 cut(s) 237
MseI TTAA 2 cut(s) 269, 648
MslI CAYNNNNRTG 4 cut(s) 474, 480, 717, 932
MspI CCGG 2 cut(s) 25, 168
MspR9I CCNGG 1 cut(s) 306
MunI CAATTG 2 cut(s) 447, 486
Mva1269I GAATGC 1 cut(s) 1043
MvaI CCWGG 1 cut(s) 306
MvnI CGCG 1 cut(s) 246
MwoI GCNNNNNNNGC 2 cut(s) 252, 626
NdeII GATC 3 cut(s) 664, 847, 949
NlaIII CATG 4 cut(s) 279, 479, 716, 956
NlaIV GGNNCC 3 cut(s) 19, 303, 666
NmuCI GTSAC 3 cut(s) 183, 767, 776
NsiI ATGCAT 1 cut(s) 942
NspI RCATGY 1 cut(s) 279
PagI TCATGA 1 cut(s) 952
PciI ACATGT 1 cut(s) 275
PcsI WCGNNNNNNNCGW 1 cut(s) 724
PctI GAATGC 1 cut(s) 1043
PdmI GAANNNNTTC 1 cut(s) 237
PfeI GAWTC 1 cut(s) 826
PkrI GCNGC 4 cut(s) 254, 298, 628, 843
PleI GAGTC 2 cut(s) 318, 577
PpsI GAGTC 2 cut(s) 318, 577
PpuMI RGGWCCY 1 cut(s) 98
PscI ACATGT 1 cut(s) 275
Psp5II RGGWCCY 1 cut(s) 98
Psp6I CCWGG 1 cut(s) 304
PspFI CCCAGC 1 cut(s) 555
PspGI CCWGG 1 cut(s) 304
PspN4I GGNNCC 3 cut(s) 19, 303, 666
PspPI GGNCC 3 cut(s) 27, 98, 553
PspPPI RGGWCCY 1 cut(s) 98
PstNI CAGNNNCTG 1 cut(s) 101
PsuI RGATCY 1 cut(s) 664
RsaI GTAC 6 cut(s) 220, 226, 678, 897, 1008, 1035
RsaNI GTAC 6 cut(s) 219, 225, 677, 896, 1007, 1034
RseI CAYNNNNRTG 4 cut(s) 474, 480, 717, 932
SaqAI TTAA 2 cut(s) 269, 648
SatI GCNGC 4 cut(s) 253, 297, 627, 842
Sau3AI GATC 3 cut(s) 664, 847, 949
Sau96I GGNCC 3 cut(s) 27, 98, 553
SchI GAGTC 2 cut(s) 318, 577
ScrFI CCNGG 1 cut(s) 306
SduI GDGCHC 2 cut(s) 20, 415
SexAI ACCWGGT 1 cut(s) 304
SfaNI GCATC 7 cut(s) 345, 376, 442, 472, 823, 927, 949
SgrAI CRCCGGYG 1 cut(s) 167
SinI GGWCC 1 cut(s) 98
SmiMI CAYNNNNRTG 4 cut(s) 474, 480, 717, 932
SpeI ACTAGT 1 cut(s) 227
Sse9I AATT 7 cut(s) 129, 154, 237, 447, 486, 644, 980
SsiI CCGC 3 cut(s) 299, 455, 841
SspMI CTAG 3 cut(s) 228, 354, 1048
StyD4I CCNGG 1 cut(s) 304
StyI CCWWGG 3 cut(s) 353, 733, 878
TaaI ACNGT 4 cut(s) 183, 198, 410, 815
TaiI ACGT 1 cut(s) 774
TaqI TCGA 1 cut(s) 290
TaqII GACCGA 1 cut(s) 840
TasI AATT 7 cut(s) 129, 154, 237, 447, 486, 644, 980
TatI WGTACW 2 cut(s) 676, 1006
TauI GCSGC 1 cut(s) 844
TfiI GAWTC 1 cut(s) 826
Tru1I TTAA 2 cut(s) 269, 648
Tru9I TTAA 2 cut(s) 269, 648
TscAI CASTG 3 cut(s) 415, 781, 934
TseFI GTSAC 3 cut(s) 183, 767, 776
TseI GCWGC 3 cut(s) 252, 296, 626
Tsp45I GTSAC 3 cut(s) 183, 767, 776
TspDTI ATGAA 3 cut(s) 590, 969, 992
TspGWI ACGGA 2 cut(s) 68, 913
TspRI CASTG 3 cut(s) 415, 781, 934
VpaK11BI GGWCC 1 cut(s) 98
XapI RAATTY 2 cut(s) 129, 237
XceI RCATGY 1 cut(s) 279
XmaJI CCTAGG 1 cut(s) 353
XmiI GTMKAC 1 cut(s) 854
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 3 cut(s) 228, 354, 1048
ZraI GACGTC 1 cut(s) 772
Zsp2I ATGCAT 1 cut(s) 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.