MD09G1194900.v1.1
NAC Family

inactive protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001566
Physical Location & Seq
Reverse (-)
17568709 .. 17574084
5376 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1194900.v1.1.491

Sequence Viewer

Length: 2256 bp
ATGAGTCGAGAACAGAAGCGGGGGAAGCAGGAGAAGGGCTCTGATGACGCTGAGAAGGTTGTCGTCGCGGTTAAGGCCTCGAAGGAAGTTCCTAAGACTGCTCTGGTCTGGGCCTTGACTCATGTTGTTCAACCCGGGGACTGCATAACACTGCTTGTGGTTGTGCCGTCTCAAAGTTCAGGTAAAAAGTTATGGGGCTTCCCAAGATTTGCAGGGGATTGCGCCAATCACCGGAAGTCTCATACAGGAACAACCTCAGAGCTGAAGTGCGATATATCAGATTCCTGCTCACAGATGATCCTTCAGCTTCATGAAGTTTATGATCCAAATAAGATAAATGTCAAGATAAAAATTATTTCTGGATCACCTTCTGGTTCTGTGGCGGTCGAGGTCAAAAAAGCTCAAGCCAGTTGGGTTGTTCTAGACAAACATCTTAAACATGAGGAAAAACACTGCATGGAAGAGCTGCAGTGCAACATTGTGGTAATGAAGCGTTCCCAGCCAAAAGTTCTTCGCTTGAATTTGAATGGATCATCTAAGAAGGAACCTGAATTGGCCAGTTCCTTGCTGTCTGAGCATGGTGCAGGGTCTGATGAACATGCCAAGCAGAAGAATGATTCTTTGAGTTCCATTCGAGAGCTGGTTGTGACTCCAACTAGTAGTCCAGAGCTTGGGACGCCATTTACAGCCACCGAGGCTGGAACTTCATCAGTGTCAAGTTCAGATCCAGGAACTTCACCTTTTTTCATTCCAGAAATAAATGAAGATCTGAAGAAAGAGGAATCGTTAGTTAATAAAGAAAACAAGGTTCTTGATGACAGTAGTTCAGACACAGACAGTGAACATTTATCAGCTTCTTCAAGAAGTTTGAGGTTCCAACCATGGATAGCTGAATTTCTTAATTCTCATCGCCCATACTCGCAACACATGGAAGACAGCTCACACAGAAGTAACGATAATTCAAAAGCATCTACTACCAAGGATTTGCTGAAATTTTCAAAACTTGATAAAGATGCTGGAATTGGAATGCGAAATTTTAGAGTTGACATGGAGTTCAGCGGAAATCTGAGAGAAGCAATTTCACTGTCTAGTAATGCTCCTCCAGGCCCTCCTCCATTATGTTCAATATGCCAACACAAGGCACCTGTTTTTGGAAAACCTCCAAGGTGGTTTAGCTATGCAGAGCTGGAGCTTGCTACTGGAGGATTTTCGCAAGCCAATTTTTTGGCCGAAGGAGGGTTTGGTTCTGTTCACAGAGGGGTGCTACCAGATGGCCAGGCTGTTGCTGTCAAGCAACATAAATTGGCTAGTTCTCAGGGGGATCAGGAGTTCTGTTCAGAAGTAGAAGTCCTGAGCTGTGCTCAGCATCGAAATGTTGTCATGTTGGTCGGCTTCTGTATCGAGGACAAAAGAAGATTGCTGGTCTATGAATATATATGCAATGGGTCATTAGATTCTCATCTATACAGGCGACATTGTGAGCCTTTAGAATGGTCTGCACGGCAAAAGATTGCTGTAGGGGCAGCTCGAGGGCTACGATATCTTCATGAAGAATGCAGAGTGGGATGCATTGTGCACCGTGACATGCGGCCAAACAACATCCTCATCACCCATGATTTTGAGCCACTGGTTGGGGATTTTGGCCTCGCAAGGTGGCAGCCTGATGGGGACACGGGTGTGGACACGAGGGTCATTGGAACATTCGGATATTTGGCTCCTGAATATGCTCAGAGTGGTCAAATCACAGAAAAGGCTGATGTTTATTCCTTCGGGGTGGTCTTGGTGGAACTTGTTACAGGGCGAAAAGCTGTGGACCTTGACCGGCCTAAGGGGCAGCAATGTCTCACTGAATGGGCACGGCCGTTGTTAGAAGAATATGCCATTGATGAACTGATTGACCCAAGACTTGACAACTTCTATTCCGAACACGAGATTTACTGCATGCTGCACGCTGCTTCGCTATGTATACGGCGGGATCCTCAATCTAGGCCTCGCATGTCTCAGGTTCTCCGTATACTAGAAGGTGACATGGTGATGGATACAAATTATATGTCAACGGCCGGGTACGATGTAGGATGCCGGAATGGTCACGATGTGGGATGCCGGAGTGGTCGACTTTGGTCAGAGCAGCAGCAGCAGAAGGAACATCATAGCGGCCCTATACTCGACGAAGCGATGGACGGGTACGAGAAGCTCTCTCGTGAGAATTTAAGGCCAGGTTTCAGGGAGAGGGACAGAGTAAGGCGGACTCCATGA

Protein Analysis

752

Amino Acids

83.66

Weight (kDa)

6.27

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 407 - 672 2.8e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 407 - 670 5.3e-40 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1688
AccB1I GGYRCC 1 cut(s) 1141
AccB7I CCANNNNNTGG 2 cut(s) 671, 1631
AccI GTMKAC 3 cut(s) 1966, 2014, 2113
AccII CGCG 1 cut(s) 68
AciI CCGC 8 cut(s) 19, 68, 383, 1059, 1588, 1972, 2154, 2245
AclWI GGATC 8 cut(s) 292, 317, 370, 538, 719, 1329, 1970, 1983
AcoI YGGCCR 6 cut(s) 555, 1227, 1273, 1589, 1859, 2058
AcsI RAATTY 5 cut(s) 520, 893, 992, 1033, 2206
AcuI CTGAAG 3 cut(s) 284, 287, 791
AcyI GRCGYC 1 cut(s) 677
AdeI CACNNNGTG 1 cut(s) 2095
AfaI GTAC 2 cut(s) 2066, 2186
AfiI CCNNNNNNNGG 7 cut(s) 231, 671, 1138, 1151, 1236, 1631, 1828
AgsI TTSAA 7 cut(s) 131, 520, 526, 861, 963, 999, 1125
AhlI ACTAGT 1 cut(s) 656
AjnI CCWGG 4 cut(s) 727, 1102, 1275, 2215
AjuI GAANNNNNNNTTGG 2 cut(s) 1224, 1256
AleI CACNNNNGTG 1 cut(s) 1676
AloI GAACNNNNNNTCC 2 cut(s) 1313, 1345
Alw21I GWGCWC 2 cut(s) 1363, 1578
Alw26I GTCTC 4 cut(s) 174, 243, 1847, 2004
Alw44I GTGCAC 1 cut(s) 1574
AlwI GGATC 8 cut(s) 292, 317, 370, 538, 719, 1329, 1970, 1983
AlwNI CAGNNNCTG 1 cut(s) 590
Ama87I CYCGRG 2 cut(s) 134, 1527
ApaLI GTGCAC 1 cut(s) 1574
ApeKI GCWGC 9 cut(s) 466, 1523, 1657, 1834, 1945, 1952, 2128, 2131, 2134
ApoI RAATTY 5 cut(s) 520, 893, 992, 1033, 2206
ArsI GACNNNNNNTTYG 2 cut(s) 2036, 2068
AspLEI GCGC 1 cut(s) 224
AspS9I GGNCC 4 cut(s) 111, 1106, 1813, 2156
AsuC2I CCSGG 3 cut(s) 135, 136, 2062
AsuHPI GGTGA 6 cut(s) 221, 357, 729, 1600, 2036, 2044
AvaI CYCGRG 2 cut(s) 134, 1527
AvaII GGWCC 1 cut(s) 1813
AxyI CCTNAGG 1 cut(s) 1827
BaeGI GKGCMC 2 cut(s) 1578, 1858
BalI TGGCCA 2 cut(s) 557, 1275
BamHI GGATCC 1 cut(s) 1975
BanI GGYRCC 1 cut(s) 1141
BanII GRGCYC 1 cut(s) 41
BauI CACGAG 3 cut(s) 1684, 1928, 2199
BbsI GAAGAC 1 cut(s) 939
Bbv12I GWGCWC 2 cut(s) 1363, 1578
BbvI GCAGC 9 cut(s) 453, 1535, 1669, 1846, 1932, 1939, 2140, 2143, 2146
BccI CCATC 4 cut(s) 1265, 1658, 2029, 2170
BceAI ACGGC 6 cut(s) 151, 1517, 1846, 1874, 1985, 2073
BciT130I CCWGG 4 cut(s) 729, 1104, 1277, 2217
BciVI GTATCC 1 cut(s) 2032
BcnI CCSGG 3 cut(s) 135, 136, 2062
BcoDI GTCTC 4 cut(s) 174, 243, 1847, 2004
BcuI ACTAGT 1 cut(s) 656
BfaI CTAG 6 cut(s) 422, 657, 1089, 1308, 1986, 2018
BfmI CTRYAG 2 cut(s) 467, 1515
BfuI GTATCC 1 cut(s) 2032
BglI GCCNNNNNGGC 2 cut(s) 695, 1831
BglII AGATCT 1 cut(s) 766
BlpI GCTNAGC 1 cut(s) 1362
Bme1390I CCNGG 7 cut(s) 135, 136, 729, 1104, 1277, 2062, 2217
Bme18I GGWCC 1 cut(s) 1813
BmeT110I CYCGRG 2 cut(s) 134, 1527
BmgT120I GGNCC 4 cut(s) 111, 1106, 1813, 2156
BmiI GGNNCC 5 cut(s) 546, 875, 1143, 1716, 1977
BmrFI CCNGG 7 cut(s) 135, 136, 729, 1104, 1277, 2062, 2217
BmsI GCATC 6 cut(s) 977, 1003, 1375, 1556, 2066, 2090
BoxI GACNNNNGTC 1 cut(s) 2119
BpiI GAAGAC 1 cut(s) 939
BplI GAGNNNNNCTC 6 cut(s) 23, 55, 1345, 1377, 2180, 2212
BpmI CTGGAG 3 cut(s) 1086, 1208, 1221
Bpu10I CCTNAGC 1 cut(s) 1352
Bpu1102I GCTNAGC 1 cut(s) 1362
BpuEI CTTGAG 1 cut(s) 387
BpuMI CCSGG 3 cut(s) 135, 136, 2062
BsaBI GATNNNNATC 1 cut(s) 1458
BsaHI GRCGYC 1 cut(s) 677
BsaJI CCNNGG 6 cut(s) 134, 135, 693, 881, 978, 1163
BsaWI WCCGGW 1 cut(s) 231
Bsc4I CCNNNNNNNGG 7 cut(s) 231, 671, 1138, 1151, 1236, 1631, 1828
Bse118I RCCGGY 1 cut(s) 1821
Bse1I ACTGG 4 cut(s) 408, 558, 1204, 1632
Bse21I CCTNAGG 1 cut(s) 1827
Bse3DI GCAATG 2 cut(s) 1447, 1844
Bse8I GATNNNNATC 1 cut(s) 1458
BseBI CCWGG 4 cut(s) 729, 1104, 1277, 2217
BseDI CCNNGG 6 cut(s) 134, 135, 693, 881, 978, 1163
BseGI GGATG 4 cut(s) 1571, 1599, 2081, 2105
BseJI GATNNNNATC 1 cut(s) 1458
BseLI CCNNNNNNNGG 7 cut(s) 231, 671, 1138, 1151, 1236, 1631, 1828
BseMI GCAATG 2 cut(s) 1447, 1844
BseMII CTCAG 9 cut(s) 42, 270, 564, 1058, 1328, 1343, 1376, 1742, 2015
BseNI ACTGG 4 cut(s) 408, 558, 1204, 1632
BseRI GAGGAG 2 cut(s) 1089, 1101
BseSI GKGCMC 2 cut(s) 1578, 1858
BseX3I CGGCCG 2 cut(s) 1859, 2058
BseXI GCAGC 9 cut(s) 453, 1535, 1669, 1846, 1932, 1939, 2140, 2143, 2146
BseYI CCCAGC 1 cut(s) 498
BsgI GTGCAG 3 cut(s) 603, 1482, 1931
Bsh1236I CGCG 1 cut(s) 68
Bsh1285I CGRYCG 3 cut(s) 387, 1862, 2061
BshNI GGYRCC 1 cut(s) 1141
BsiEI CGRYCG 3 cut(s) 387, 1862, 2061
BsiHKAI GWGCWC 2 cut(s) 1363, 1578
BsiHKCI CYCGRG 2 cut(s) 134, 1527
BsiSI CCGG 6 cut(s) 135, 232, 1822, 2061, 2080, 2104
BslFI GGGAC 4 cut(s) 152, 688, 1682, 2246
BslI CCNNNNNNNGG 7 cut(s) 231, 671, 1138, 1151, 1236, 1631, 1828
BsmAI GTCTC 4 cut(s) 174, 243, 1847, 2004
BsmBI CGTCTC 1 cut(s) 174
BsmFI GGGAC 4 cut(s) 152, 688, 1682, 2246
BsmI GAATGC 2 cut(s) 1032, 1559
BsoBI CYCGRG 2 cut(s) 134, 1527
Bsp1286I GDGCHC 4 cut(s) 41, 1363, 1578, 1858
Bsp143I GATC 8 cut(s) 297, 322, 362, 530, 724, 766, 1321, 1975
Bsp1720I GCTNAGC 1 cut(s) 1362
Bsp19I CCATGG 1 cut(s) 881
BspACI CCGC 8 cut(s) 19, 68, 383, 1059, 1588, 1972, 2154, 2245
BspCNI CTCAG 9 cut(s) 43, 269, 565, 1059, 1327, 1344, 1375, 1741, 2014
BspFNI CGCG 1 cut(s) 68
BspHI TCATGA 2 cut(s) 310, 1546
BspLI GGNNCC 5 cut(s) 546, 875, 1143, 1716, 1977
BspMAI CTGCAG 1 cut(s) 471
BspPI GGATC 8 cut(s) 292, 317, 370, 538, 719, 1329, 1970, 1983
BspQI GCTCTTC 1 cut(s) 456
BspT107I GGYRCC 1 cut(s) 1141
BsrDI GCAATG 2 cut(s) 1447, 1844
BsrFI RCCGGY 1 cut(s) 1821
BsrI ACTGG 4 cut(s) 408, 558, 1204, 1632
BssAI RCCGGY 1 cut(s) 1821
BssECI CCNNGG 6 cut(s) 134, 135, 693, 881, 978, 1163
BssMI GATC 8 cut(s) 297, 322, 362, 530, 724, 766, 1321, 1975
BssNAI GTATAC 2 cut(s) 1967, 2015
BssNI GRCGYC 1 cut(s) 677
BssSI CACGAG 3 cut(s) 1684, 1928, 2199
BssT1I CCWWGG 3 cut(s) 881, 978, 1163
Bst1107I GTATAC 2 cut(s) 1967, 2015
Bst2BI CACGAG 3 cut(s) 1684, 1928, 2199
Bst2UI CCWGG 4 cut(s) 729, 1104, 1277, 2217
Bst4CI ACNGT 4 cut(s) 821, 839, 1086, 1580
Bst6I CTCTTC 1 cut(s) 456
BstACI GRCGYC 1 cut(s) 677
BstC8I GCNNGC 4 cut(s) 1194, 1215, 1943, 1950
BstDSI CCRYGG 1 cut(s) 881
BstF5I GGATG 4 cut(s) 1571, 1599, 2081, 2105
BstFNI CGCG 1 cut(s) 68
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 8 cut(s) 300, 325, 365, 533, 727, 769, 1324, 1978
BstMAI GTCTC 4 cut(s) 174, 243, 1847, 2004
BstMBI GATC 8 cut(s) 297, 322, 362, 530, 724, 766, 1321, 1975
BstMCI CGRYCG 3 cut(s) 387, 1862, 2061
BstMWI GCNNNNNNNGC 9 cut(s) 25, 74, 499, 574, 676, 695, 1520, 1831, 2134
BstNI CCWGG 4 cut(s) 729, 1104, 1277, 2217
BstNSI RCATGY 4 cut(s) 602, 1588, 1945, 1999
BstPAI GACNNNNGTC 1 cut(s) 2119
BstSCI CCNGG 7 cut(s) 133, 134, 727, 1102, 1275, 2060, 2215
BstSFI CTRYAG 2 cut(s) 467, 1515
BstSLI GKGCMC 2 cut(s) 1578, 1858
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 9 cut(s) 453, 1535, 1669, 1846, 1932, 1939, 2140, 2143, 2146
BstV2I GAAGAC 1 cut(s) 939
BstX2I RGATCY 3 cut(s) 724, 766, 1975
BstXI CCANNNNNNTGG 1 cut(s) 1225
BstYI RGATCY 3 cut(s) 724, 766, 1975
BstZ17I GTATAC 2 cut(s) 1967, 2015
BstZI CGGCCG 2 cut(s) 1859, 2058
Bsu36I CCTNAGG 1 cut(s) 1827
BsuI GTATCC 1 cut(s) 2032
BtgI CCRYGG 1 cut(s) 881
BtgZI GCGATG 2 cut(s) 893, 2189
BtsCI GGATG 4 cut(s) 1571, 1599, 2081, 2105
BtsI GCAGTG 3 cut(s) 149, 451, 476
BtsIMutI CAGTG 8 cut(s) 149, 451, 476, 717, 844, 1082, 1625, 1845
Cac8I GCNNGC 4 cut(s) 1194, 1215, 1943, 1950
CaiI CAGNNNCTG 1 cut(s) 590
CciI TCATGA 2 cut(s) 310, 1546
CfoI GCGC 1 cut(s) 224
Cfr10I RCCGGY 1 cut(s) 1821
Cfr13I GGNCC 4 cut(s) 111, 1106, 1813, 2156
Cfr9I CCCGGG 1 cut(s) 134
CseI GACGC 2 cut(s) 56, 685
Csp6I GTAC 2 cut(s) 2065, 2185
CviQI GTAC 2 cut(s) 2065, 2185
DpnI GATC 8 cut(s) 299, 324, 364, 532, 726, 768, 1323, 1977
DpnII GATC 8 cut(s) 297, 322, 362, 530, 724, 766, 1321, 1975
DraIII CACNNNGTG 1 cut(s) 2095
DrdI GACNNNNNNGTC 1 cut(s) 1688
DseDI GACNNNNNNGTC 1 cut(s) 1688
EaeI YGGCCR 6 cut(s) 555, 1227, 1273, 1589, 1859, 2058
EagI CGGCCG 2 cut(s) 1859, 2058
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
EclXI CGGCCG 2 cut(s) 1859, 2058
Eco130I CCWWGG 3 cut(s) 881, 978, 1163
Eco147I AGGCCT 2 cut(s) 77, 1990
Eco24I GRGCYC 1 cut(s) 41
Eco32I GATATC 1 cut(s) 1541
Eco47I GGWCC 1 cut(s) 1813
Eco52I CGGCCG 2 cut(s) 1859, 2058
Eco57I CTGAAG 3 cut(s) 284, 287, 791
Eco81I CCTNAGG 1 cut(s) 1827
Eco88I CYCGRG 2 cut(s) 134, 1527
EcoO109I RGGNCCY 1 cut(s) 1106
EcoRII CCWGG 4 cut(s) 727, 1102, 1275, 2215
EcoRV GATATC 1 cut(s) 1541
EcoT14I CCWWGG 3 cut(s) 881, 978, 1163
EcoT22I ATGCAT 1 cut(s) 1571
EcoT38I GRGCYC 1 cut(s) 41
ErhI CCWWGG 3 cut(s) 881, 978, 1163
Esp3I CGTCTC 1 cut(s) 174
FaqI GGGAC 4 cut(s) 152, 688, 1682, 2246
FauI CCCGC 2 cut(s) 12, 1965
FblI GTMKAC 3 cut(s) 1966, 2014, 2113
FokI GGATG 4 cut(s) 1578, 1586, 2088, 2112
FriOI GRGCYC 1 cut(s) 41
FspBI CTAG 6 cut(s) 422, 657, 1089, 1308, 1986, 2018
GlaI GCGC 1 cut(s) 223
GsaI CCCAGC 1 cut(s) 502
GsuI CTGGAG 3 cut(s) 1086, 1208, 1221
HapII CCGG 6 cut(s) 135, 232, 1822, 2061, 2080, 2104
HgaI GACGC 2 cut(s) 56, 685
HhaI GCGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 677
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HincII GTYRAC 3 cut(s) 1045, 2055, 2114
HindII GTYRAC 3 cut(s) 1045, 2055, 2114
HinfI GANTC 8 cut(s) 4, 118, 281, 617, 649, 782, 1454, 2248
HpaII CCGG 6 cut(s) 135, 232, 1822, 2061, 2080, 2104
HphI GGTGA 6 cut(s) 221, 357, 729, 1600, 2036, 2044
Hpy99I CGWCG 2 cut(s) 68, 2171
HpyCH4III ACNGT 4 cut(s) 821, 839, 1086, 1580
HpyF10VI GCNNNNNNNGC 9 cut(s) 25, 74, 499, 574, 676, 695, 1520, 1831, 2134
Hsp92I GRCGYC 1 cut(s) 677
HspAI GCGC 1 cut(s) 222
Kzo9I GATC 8 cut(s) 297, 322, 362, 530, 724, 766, 1321, 1975
LguI GCTCTTC 1 cut(s) 456
LmnI GCTCC 3 cut(s) 1102, 1189, 1720
Lsp1109I GCAGC 9 cut(s) 453, 1535, 1669, 1846, 1932, 1939, 2140, 2143, 2146
LweI GCATC 6 cut(s) 977, 1003, 1375, 1556, 2066, 2090
MaeI CTAG 6 cut(s) 422, 657, 1089, 1308, 1986, 2018
MaeIII GTNAC 6 cut(s) 646, 950, 1580, 1792, 2024, 2087
MalI GATC 8 cut(s) 299, 324, 364, 532, 726, 768, 1323, 1977
MboI GATC 8 cut(s) 297, 322, 362, 530, 724, 766, 1321, 1975
MflI RGATCY 3 cut(s) 724, 766, 1975
MhlI GDGCHC 4 cut(s) 41, 1363, 1578, 1858
MlsI TGGCCA 2 cut(s) 557, 1275
MluNI TGGCCA 2 cut(s) 557, 1275
MlyI GAGTC 4 cut(s) 13, 112, 643, 2242
MmeI TCCRAC 2 cut(s) 677, 901
Mox20I TGGCCA 2 cut(s) 557, 1275
Mph1103I ATGCAT 1 cut(s) 1571
MscI TGGCCA 2 cut(s) 557, 1275
MseI TTAA 5 cut(s) 72, 435, 792, 900, 2210
MslI CAYNNNNRTG 3 cut(s) 1371, 1676, 2033
Msp20I TGGCCA 2 cut(s) 557, 1275
MspA1I CMGCKG 1 cut(s) 1059
MspI CCGG 6 cut(s) 135, 232, 1822, 2061, 2080, 2104
MspR9I CCNGG 7 cut(s) 135, 136, 729, 1104, 1277, 2062, 2217
Mva1269I GAATGC 2 cut(s) 1032, 1559
MvaI CCWGG 4 cut(s) 729, 1104, 1277, 2217
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 9 cut(s) 25, 74, 499, 574, 676, 695, 1520, 1831, 2134
NciI CCSGG 3 cut(s) 135, 136, 2062
NcoI CCATGG 1 cut(s) 881
NdeII GATC 8 cut(s) 297, 322, 362, 530, 724, 766, 1321, 1975
NlaIV GGNNCC 5 cut(s) 546, 875, 1143, 1716, 1977
NmuCI GTSAC 4 cut(s) 646, 1580, 2024, 2087
NsiI ATGCAT 1 cut(s) 1571
NspI RCATGY 4 cut(s) 602, 1588, 1945, 1999
OliI CACNNNNGTG 1 cut(s) 1676
PaeI GCATGC 1 cut(s) 1945
PaeR7I CTCGAG 1 cut(s) 1527
PagI TCATGA 2 cut(s) 310, 1546
PceI AGGCCT 2 cut(s) 77, 1990
PciSI GCTCTTC 1 cut(s) 456
PcsI WCGNNNNNNNCGW 1 cut(s) 1534
PctI GAATGC 2 cut(s) 1032, 1559
PfeI GAWTC 4 cut(s) 281, 617, 782, 1454
PflMI CCANNNNNTGG 2 cut(s) 671, 1631
PfoI TCCNGGA 1 cut(s) 727
PleI GAGTC 4 cut(s) 12, 112, 643, 2242
PpsI GAGTC 4 cut(s) 12, 112, 643, 2242
PshAI GACNNNNGTC 1 cut(s) 2119
Psp6I CCWGG 4 cut(s) 727, 1102, 1275, 2215
PspFI CCCAGC 1 cut(s) 498
PspGI CCWGG 4 cut(s) 727, 1102, 1275, 2215
PspN4I GGNNCC 5 cut(s) 546, 875, 1143, 1716, 1977
PspPI GGNCC 4 cut(s) 111, 1106, 1813, 2156
PspXI VCTCGAGB 1 cut(s) 1527
PstI CTGCAG 1 cut(s) 471
PstNI CAGNNNCTG 1 cut(s) 590
PsuI RGATCY 3 cut(s) 724, 766, 1975
RsaI GTAC 2 cut(s) 2066, 2186
RsaNI GTAC 2 cut(s) 2065, 2185
RseI CAYNNNNRTG 3 cut(s) 1371, 1676, 2033
SalI GTCGAC 1 cut(s) 2112
SapI GCTCTTC 1 cut(s) 456
SaqAI TTAA 5 cut(s) 72, 435, 792, 900, 2210
Sau3AI GATC 8 cut(s) 297, 322, 362, 530, 724, 766, 1321, 1975
Sau96I GGNCC 4 cut(s) 111, 1106, 1813, 2156
SchI GAGTC 4 cut(s) 13, 112, 643, 2242
ScrFI CCNGG 7 cut(s) 135, 136, 729, 1104, 1277, 2062, 2217
SduI GDGCHC 4 cut(s) 41, 1363, 1578, 1858
SfaNI GCATC 6 cut(s) 977, 1003, 1375, 1556, 2066, 2090
SfcI CTRYAG 2 cut(s) 467, 1515
Sfr274I CTCGAG 1 cut(s) 1527
SinI GGWCC 1 cut(s) 1813
SlaI CTCGAG 1 cut(s) 1527
SmaI CCCGGG 1 cut(s) 136
SmiMI CAYNNNNRTG 3 cut(s) 1371, 1676, 2033
SmlI CTYRAG 2 cut(s) 402, 1527
SmoI CTYRAG 2 cut(s) 402, 1527
SpeI ACTAGT 1 cut(s) 656
SphI GCATGC 1 cut(s) 1945
SseBI AGGCCT 2 cut(s) 77, 1990
SsiI CCGC 8 cut(s) 19, 68, 383, 1059, 1588, 1972, 2154, 2245
SspMI CTAG 6 cut(s) 422, 657, 1089, 1308, 1986, 2018
StuI AGGCCT 2 cut(s) 77, 1990
StyD4I CCNGG 7 cut(s) 133, 134, 727, 1102, 1275, 2060, 2215
StyI CCWWGG 3 cut(s) 881, 978, 1163
TaaI ACNGT 4 cut(s) 821, 839, 1086, 1580
TaqI TCGA 9 cut(s) 7, 80, 387, 634, 1369, 1401, 1528, 2113, 2166
TauI GCSGC 2 cut(s) 1591, 2157
TfiI GAWTC 4 cut(s) 281, 617, 782, 1454
Tru1I TTAA 5 cut(s) 72, 435, 792, 900, 2210
Tru9I TTAA 5 cut(s) 72, 435, 792, 900, 2210
TscAI CASTG 8 cut(s) 156, 458, 476, 717, 844, 1089, 1632, 1852
TseFI GTSAC 4 cut(s) 646, 1580, 2024, 2087
TseI GCWGC 9 cut(s) 466, 1523, 1657, 1834, 1945, 1952, 2128, 2131, 2134
Tsp45I GTSAC 4 cut(s) 646, 1580, 2024, 2087
TspGWI ACGGA 1 cut(s) 2000
TspMI CCCGGG 1 cut(s) 134
TspRI CASTG 8 cut(s) 156, 458, 476, 717, 844, 1089, 1632, 1852
Van91I CCANNNNNTGG 2 cut(s) 671, 1631
VneI GTGCAC 1 cut(s) 1574
VpaK11BI GGWCC 1 cut(s) 1813
XapI RAATTY 5 cut(s) 520, 893, 992, 1033, 2206
XbaI TCTAGA 1 cut(s) 421
XceI RCATGY 4 cut(s) 602, 1588, 1945, 1999
XcmI CCANNNNNNNNNTGG 1 cut(s) 637
XhoI CTCGAG 1 cut(s) 1527
XmaI CCCGGG 1 cut(s) 134
XmiI GTMKAC 3 cut(s) 1966, 2014, 2113
XspI CTAG 6 cut(s) 422, 657, 1089, 1308, 1986, 2018
Zsp2I ATGCAT 1 cut(s) 1571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.