MD17G1175400.v1.1
NAC Family

inactive protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001566
Physical Location & Seq
Reverse (-)
19644118 .. 19649056
4939 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1175400.v1.1.491

Sequence Viewer

Length: 2280 bp
ATGAGTCGAGAACCGAAGCGGGGGAGGCAGGAGAAGGGCTCCGATGACGCTGAGAAGGTTGTTGTCACGGTTAAGGCCTCCAAAGAAGTTCCTAAGACTGCTCTGGTCTGGGCCTTGACTCATGTTGTTCAACCTGGTGACTGTATAACACTACTTGTGGTTGTGCCCTCTCAAAGTTCAGGTAAAAAGTTATGGGGCTTCCCAAGATTTGCAGGGGATTGTGCCAATGGTCACCGGAAGTCTCATACAGGAACAACCTCTGAGCTGAAATGCGATATATCAGATTCCTGCTCTCAGATGATCCTTCAGCTCCATGAAGTTTATGATCCAAATAAGATAAATGTCAAGATAAAAATTATTTCTGGATCACCTTCTGGTTCTGTGGCGGTAGAGGCTAAAAAAGCTCAAGCCAGTTGGGTTGTTTTAGACAAACATCTAAAACATGAGGAAAAACACTGCATGGAAGAGCTGCAGTGCAACATTGTGGTTATGAAGCGTTCCCAGCCAAAGGTTCTTCGCTTGAATTTGAATGGATCATCTAAAAAGGAACCTGAATTGGCCAGTTCCTTGCCGTCTGAGCATGGTGCAGGGTCTGATAAACATCCCAACCAGAAGAATAATTCTTTGAGTTCCATTCGAGGGCCGGTTGTGACTCCAACTAGTAGTCCAGAGCTTGGGACACCATTCACAGCAACTGAGGCTGGTACCTCATCGGTGTCAAGTTCAGATCCAGGAACTTCACCTTTTTTCATTCCAGAAAAAATTGAAGATCTGAAGAAAGTGGAATCATTAGTTAGTAAAGAAAACAAAGTACTTGATGACAGTAGTTCAGACACAGATAGTGAACATTTATCATCTTCAGGTAGTATGAGGTTCCAACCATGGATAGCTGAATTTCTTGATTCTCATCATCCATCCTCGCAACACATGGAAGAAAGCTCACACAGAAGTAACGATAACTCGAAAGCATCTACTTCCAAGGCCTTTTTAGTGAAATTTTCAAAAATTGATAGAGATGCTGGAATTGGAATGCCCAATTATAGAGCTGACATGGAGTTCAGTGGAAATCTGAGAGAAGCAATTTCACTGTCCAGAAATGCTCCTCCAGGCCCCCCTCCATTATGTTCAATATGTCAACACAAGGCACCTGTATTTGGAAAGCCTCCAAGGTGGTTCAGCTATGCAGAGCTCGAGCTTGCTACCGGAGGATTTTCACAAGCCAATTTTTTGGCCGAAGGAGGTTTTGGTTCCGTTCACAGAGGGGTGCTACCGGATGGCCAGGCTGTTGCTGTCAAGCAACACAAATTGGCTAGTTCTCAGGGTGATCAGGAGTTCTGTTCAGAAGTAGAAGTCCTGAGCTGTGCTCAGCACCGAAATGTTGTCATGTTGATTGGATTCTGTATCGAGGATAAAAGAAGATTGCTGGTCTATGAGTATATATGCAATGGGTCATTGGATTCTCATCTATACGGGCGACATCGTGAGCCTTTAGAATGGTCTGCACGGCAAAAGATTGCTATAGGGGCAGCTCGAGGGCTACGATATCTTCATGAAGAATGCAGAGTGGGTTGCATTGTGCACCGTGACATGCGACCAAACAACATCCTCATCACCCATGATTTTGAGCCACTGGTTGGGGATTTTGGCCTCGCAAGGTGGCAGCCTGATGGGGACACGGGTGTGGACACAAGGGTAATTGGAACATTCGGATATTTGGCTCCTGAATATGCTCAAAGTGGTCAAATTACAGAAAAGGCTGATGTTTATTCCTTCGGGGTGGTCTTGGTGGAACTTGTTACAGGGCGAAAAGCTGTGGACCTTAACCGGCCTAAAGGGCAGCAATGTCTCACTGAATGGGCACGCCCGTTATTAGAAGAATTTGACATTGATGAACTGATTGACCCCAGACTTGAAAACTTCTATTCCGAACATGAGGTTTACTGCATGCTGCACGCTGCTTCGCTATGTATACGGCGGGATCCTCAATCTAGGCCTCGCATGTCTCAGGTTCTGCGCATGCTAGAAGGTGACATGATGATGGACACGAATTACATGTCAACGCCCGGGTATGATGTAGGATGCCGGAATGGTCATGATGTGGGATGCCGGAGTGGCCGACTTTGGTCAGAGCAGCAGCAGCAGAAGGAACATTATAGCGGCCCGCTACTAGATGAGGCGATGGAAGGGTACGAGAAGCTCTCTCTTGAGAATTTAAGGCCAGGTTTTAGGGAGAGGGAGAGGGACAGGGCAAGGCGGACTTCATGCGAAAACCATTTGTGA

Protein Analysis

760

Amino Acids

84.37

Weight (kDa)

6.35

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 408 - 673 2.2e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 408 - 672 3e-40 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2015
Acc65I GGTACC 1 cut(s) 704
AccB1I GGYRCC 2 cut(s) 704, 1144
AccB7I CCANNNNNTGG 2 cut(s) 674, 1634
AccI GTMKAC 1 cut(s) 1969
AciI CCGC 6 cut(s) 19, 386, 1975, 2157, 2162, 2254
AclWI GGATC 7 cut(s) 295, 320, 373, 541, 722, 1973, 1986
AcoI YGGCCR 4 cut(s) 558, 1230, 1276, 2113
AcsI RAATTY 5 cut(s) 523, 893, 995, 1877, 2209
AcuI CTGAAG 3 cut(s) 290, 794, 843
AfaI GTAC 3 cut(s) 706, 813, 2189
AfiI CCNNNNNNNGG 6 cut(s) 20, 508, 639, 674, 1154, 1634
AflIII ACRYGT 1 cut(s) 2052
AgsI TTSAA 7 cut(s) 131, 523, 529, 767, 1002, 1128, 1913
AhlI ACTAGT 1 cut(s) 659
AjnI CCWGG 5 cut(s) 133, 730, 1105, 1278, 2218
AjuI GAANNNNNNNTTGG 2 cut(s) 1227, 1259
AleI CACNNNNGTG 1 cut(s) 1679
Alw21I GWGCWC 3 cut(s) 1191, 1366, 1581
Alw26I GTCTC 3 cut(s) 246, 1850, 2007
Alw44I GTGCAC 1 cut(s) 1577
AlwI GGATC 7 cut(s) 295, 320, 373, 541, 722, 1973, 1986
AlwNI CAGNNNCTG 3 cut(s) 593, 695, 2011
Ama87I CYCGRG 3 cut(s) 1190, 1530, 2063
ApaLI GTGCAC 1 cut(s) 1577
ApeKI GCWGC 9 cut(s) 469, 1526, 1660, 1837, 1948, 1955, 2131, 2134, 2137
ApoI RAATTY 5 cut(s) 523, 893, 995, 1877, 2209
ArsI GACNNNNNNTTYG 2 cut(s) 2039, 2071
Asp718I GGTACC 1 cut(s) 704
AspLEI GCGC 1 cut(s) 2016
AspS9I GGNCC 5 cut(s) 111, 641, 1109, 1816, 2159
AsuC2I CCSGG 2 cut(s) 2064, 2065
AsuHPI GGTGA 7 cut(s) 149, 224, 360, 732, 1334, 1603, 2039
AvaI CYCGRG 3 cut(s) 1190, 1530, 2063
AvaII GGWCC 1 cut(s) 1816
BaeGI GKGCMC 3 cut(s) 168, 1581, 1861
BalI TGGCCA 2 cut(s) 560, 1278
BamHI GGATCC 1 cut(s) 1978
BanI GGYRCC 2 cut(s) 704, 1144
BanII GRGCYC 2 cut(s) 41, 1191
Bbv12I GWGCWC 3 cut(s) 1191, 1366, 1581
BbvI GCAGC 9 cut(s) 456, 1538, 1672, 1849, 1935, 1942, 2143, 2146, 2149
BccI CCATC 5 cut(s) 922, 1268, 1661, 2032, 2173
BceAI ACGGC 3 cut(s) 556, 1520, 1988
BciT130I CCWGG 5 cut(s) 135, 732, 1107, 1280, 2220
BclI TGATCA 1 cut(s) 1324
BcnI CCSGG 2 cut(s) 2064, 2065
BcoDI GTCTC 3 cut(s) 246, 1850, 2007
BcuI ACTAGT 1 cut(s) 659
BfaI CTAG 5 cut(s) 660, 1311, 1989, 2021, 2168
BfmI CTRYAG 2 cut(s) 470, 1518
BglI GCCNNNNNGGC 2 cut(s) 1834, 2112
BglII AGATCT 1 cut(s) 769
BlpI GCTNAGC 1 cut(s) 1365
BmcAI AGTACT 1 cut(s) 813
Bme1390I CCNGG 7 cut(s) 135, 732, 1107, 1280, 2064, 2065, 2220
Bme18I GGWCC 1 cut(s) 1816
BmeT110I CYCGRG 3 cut(s) 1190, 1530, 2063
BmgT120I GGNCC 5 cut(s) 111, 641, 1109, 1816, 2159
BmiI GGNNCC 9 cut(s) 40, 549, 706, 875, 1111, 1146, 1249, 1719, 1980
BmrFI CCNGG 7 cut(s) 135, 732, 1107, 1280, 2064, 2065, 2220
BmsI GCATC 4 cut(s) 977, 1006, 2069, 2093
BoxI GACNNNNGTC 1 cut(s) 2122
BplI GAGNNNNNCTC 6 cut(s) 23, 55, 1348, 1380, 2183, 2215
BpmI CTGGAG 1 cut(s) 1089
Bpu10I CCTNAGC 1 cut(s) 1355
Bpu1102I GCTNAGC 1 cut(s) 1365
BpuEI CTTGAG 2 cut(s) 390, 2225
BpuMI CCSGG 2 cut(s) 2064, 2065
BsaBI GATNNNNATC 3 cut(s) 600, 906, 1461
BsaJI CCNNGG 4 cut(s) 881, 978, 1166, 2063
BsaWI WCCGGW 3 cut(s) 234, 1202, 1270
Bsc4I CCNNNNNNNGG 6 cut(s) 20, 508, 639, 674, 1154, 1634
Bse118I RCCGGY 2 cut(s) 643, 1824
Bse1I ACTGG 3 cut(s) 411, 561, 1635
Bse3DI GCAATG 2 cut(s) 1450, 1847
Bse8I GATNNNNATC 3 cut(s) 600, 906, 1461
BseBI CCWGG 5 cut(s) 135, 732, 1107, 1280, 2220
BseDI CCNNGG 4 cut(s) 881, 978, 1166, 2063
BseGI GGATG 7 cut(s) 601, 910, 914, 1279, 1602, 2084, 2108
BseJI GATNNNNATC 3 cut(s) 600, 906, 1461
BseLI CCNNNNNNNGG 6 cut(s) 20, 508, 639, 674, 1154, 1634
BseMI GCAATG 2 cut(s) 1450, 1847
BseNI ACTGG 3 cut(s) 411, 561, 1635
BseRI GAGGAG 1 cut(s) 1092
BseSI GKGCMC 3 cut(s) 168, 1581, 1861
BseXI GCAGC 9 cut(s) 456, 1538, 1672, 1849, 1935, 1942, 2143, 2146, 2149
BseYI CCCAGC 1 cut(s) 501
BsgI GTGCAG 3 cut(s) 606, 1485, 1934
BshNI GGYRCC 2 cut(s) 704, 1144
BsiHKAI GWGCWC 3 cut(s) 1191, 1366, 1581
BsiHKCI CYCGRG 3 cut(s) 1190, 1530, 2063
BsiSI CCGG 8 cut(s) 235, 644, 1203, 1271, 1825, 2064, 2083, 2107
BslFI GGGAC 3 cut(s) 691, 1685, 2255
BslI CCNNNNNNNGG 6 cut(s) 20, 508, 639, 674, 1154, 1634
BsmAI GTCTC 3 cut(s) 246, 1850, 2007
BsmFI GGGAC 3 cut(s) 691, 1685, 2255
BsmI GAATGC 2 cut(s) 1035, 1562
BsoBI CYCGRG 3 cut(s) 1190, 1530, 2063
Bsp1286I GDGCHC 6 cut(s) 41, 168, 1191, 1366, 1581, 1861
Bsp143I GATC 8 cut(s) 300, 325, 365, 533, 727, 769, 1324, 1978
Bsp1720I GCTNAGC 1 cut(s) 1365
Bsp19I CCATGG 1 cut(s) 881
BspACI CCGC 6 cut(s) 19, 386, 1975, 2157, 2162, 2254
BspHI TCATGA 2 cut(s) 1549, 2092
BspLI GGNNCC 9 cut(s) 40, 549, 706, 875, 1111, 1146, 1249, 1719, 1980
BspMAI CTGCAG 1 cut(s) 474
BspPI GGATC 7 cut(s) 295, 320, 373, 541, 722, 1973, 1986
BspQI GCTCTTC 1 cut(s) 459
BspT107I GGYRCC 2 cut(s) 704, 1144
BsrDI GCAATG 2 cut(s) 1450, 1847
BsrFI RCCGGY 2 cut(s) 643, 1824
BsrI ACTGG 3 cut(s) 411, 561, 1635
BssAI RCCGGY 2 cut(s) 643, 1824
BssECI CCNNGG 4 cut(s) 881, 978, 1166, 2063
BssMI GATC 8 cut(s) 300, 325, 365, 533, 727, 769, 1324, 1978
BssNAI GTATAC 1 cut(s) 1970
BssT1I CCWWGG 3 cut(s) 881, 978, 1166
Bst1107I GTATAC 1 cut(s) 1970
Bst2UI CCWGG 5 cut(s) 135, 732, 1107, 1280, 2220
Bst4CI ACNGT 5 cut(s) 70, 143, 824, 1089, 1583
Bst6I CTCTTC 1 cut(s) 459
BstC8I GCNNGC 6 cut(s) 1197, 1861, 1946, 1953, 2018, 2162
BstDSI CCRYGG 1 cut(s) 881
BstEII GGTNACC 1 cut(s) 230
BstF5I GGATG 7 cut(s) 601, 910, 914, 1279, 1602, 2084, 2108
BstHHI GCGC 1 cut(s) 2016
BstKTI GATC 8 cut(s) 303, 328, 368, 536, 730, 772, 1327, 1981
BstMAI GTCTC 3 cut(s) 246, 1850, 2007
BstMBI GATC 8 cut(s) 300, 325, 365, 533, 727, 769, 1324, 1978
BstNI CCWGG 5 cut(s) 135, 732, 1107, 1280, 2220
BstNSI RCATGY 5 cut(s) 1591, 1948, 2002, 2020, 2056
BstPAI GACNNNNGTC 1 cut(s) 2122
BstPI GGTNACC 1 cut(s) 230
BstSCI CCNGG 7 cut(s) 133, 730, 1105, 1278, 2062, 2063, 2218
BstSFI CTRYAG 2 cut(s) 470, 1518
BstSLI GKGCMC 3 cut(s) 168, 1581, 1861
BstV1I GCAGC 9 cut(s) 456, 1538, 1672, 1849, 1935, 1942, 2143, 2146, 2149
BstX2I RGATCY 3 cut(s) 727, 769, 1978
BstXI CCANNNNNNTGG 1 cut(s) 1228
BstYI RGATCY 3 cut(s) 727, 769, 1978
BstZ17I GTATAC 1 cut(s) 1970
BtgI CCRYGG 1 cut(s) 881
BtgZI GCGATG 1 cut(s) 2192
BtsCI GGATG 7 cut(s) 601, 910, 914, 1279, 1602, 2084, 2108
BtsI GCAGTG 2 cut(s) 454, 479
BtsIMutI CAGTG 6 cut(s) 454, 479, 1066, 1085, 1628, 1848
Cac8I GCNNGC 6 cut(s) 1197, 1861, 1946, 1953, 2018, 2162
CaiI CAGNNNCTG 3 cut(s) 593, 695, 2011
CciI TCATGA 2 cut(s) 1549, 2092
CfoI GCGC 1 cut(s) 2016
Cfr10I RCCGGY 2 cut(s) 643, 1824
Cfr13I GGNCC 5 cut(s) 111, 641, 1109, 1816, 2159
Cfr9I CCCGGG 1 cut(s) 2063
CseI GACGC 1 cut(s) 56
CsiI ACCWGGT 1 cut(s) 133
Csp6I GTAC 3 cut(s) 705, 812, 2188
CviQI GTAC 3 cut(s) 705, 812, 2188
DpnI GATC 8 cut(s) 302, 327, 367, 535, 729, 771, 1326, 1980
DpnII GATC 8 cut(s) 300, 325, 365, 533, 727, 769, 1324, 1978
EaeI YGGCCR 4 cut(s) 558, 1230, 1276, 2113
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
EciI GGCGGA 1 cut(s) 2269
Ecl136II GAGCTC 1 cut(s) 1189
Eco130I CCWWGG 3 cut(s) 881, 978, 1166
Eco147I AGGCCT 3 cut(s) 77, 983, 1993
Eco24I GRGCYC 2 cut(s) 41, 1191
Eco32I GATATC 1 cut(s) 1544
Eco47I GGWCC 1 cut(s) 1816
Eco53kI GAGCTC 1 cut(s) 1189
Eco57I CTGAAG 3 cut(s) 290, 794, 843
Eco88I CYCGRG 3 cut(s) 1190, 1530, 2063
Eco91I GGTNACC 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 1189
EcoO109I RGGNCCY 1 cut(s) 1109
EcoO65I GGTNACC 1 cut(s) 230
EcoRII CCWGG 5 cut(s) 133, 730, 1105, 1278, 2218
EcoRV GATATC 1 cut(s) 1544
EcoT14I CCWWGG 3 cut(s) 881, 978, 1166
EcoT38I GRGCYC 2 cut(s) 41, 1191
ErhI CCWWGG 3 cut(s) 881, 978, 1166
FalI AAGNNNNNCTT 2 cut(s) 2242, 2274
FaqI GGGAC 3 cut(s) 691, 1685, 2255
FauI CCCGC 3 cut(s) 12, 1968, 2169
FbaI TGATCA 1 cut(s) 1324
FblI GTMKAC 1 cut(s) 1969
FokI GGATG 7 cut(s) 588, 897, 901, 1286, 1589, 2091, 2115
FriOI GRGCYC 2 cut(s) 41, 1191
FspBI CTAG 5 cut(s) 660, 1311, 1989, 2021, 2168
FspI TGCGCA 1 cut(s) 2015
GlaI GCGC 1 cut(s) 2015
GsaI CCCAGC 1 cut(s) 505
GsuI CTGGAG 1 cut(s) 1089
HapII CCGG 8 cut(s) 235, 644, 1203, 1271, 1825, 2064, 2083, 2107
HgaI GACGC 1 cut(s) 56
HhaI GCGC 1 cut(s) 2016
Hin6I GCGC 1 cut(s) 2014
HinP1I GCGC 1 cut(s) 2014
HincII GTYRAC 2 cut(s) 1136, 2058
HindII GTYRAC 2 cut(s) 1136, 2058
HinfI GANTC 8 cut(s) 4, 118, 284, 652, 785, 902, 1395, 1457
HpaII CCGG 8 cut(s) 235, 644, 1203, 1271, 1825, 2064, 2083, 2107
HphI GGTGA 7 cut(s) 149, 224, 360, 732, 1334, 1603, 2039
Hpy166II GTNNAC 9 cut(s) 845, 1136, 1255, 1579, 1684, 1816, 1939, 1970, 2058
Hpy8I GTNNAC 9 cut(s) 845, 1136, 1255, 1579, 1684, 1816, 1939, 1970, 2058
HpyAV CCTTC 9 cut(s) 28, 49, 314, 381, 1229, 1780, 2018, 2137, 2177
HpyCH4III ACNGT 5 cut(s) 70, 143, 824, 1089, 1583
HspAI GCGC 1 cut(s) 2014
KpnI GGTACC 1 cut(s) 708
Ksp22I TGATCA 1 cut(s) 1324
Kzo9I GATC 8 cut(s) 300, 325, 365, 533, 727, 769, 1324, 1978
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 4 cut(s) 44, 315, 1105, 1723
Lsp1109I GCAGC 9 cut(s) 456, 1538, 1672, 1849, 1935, 1942, 2143, 2146, 2149
LweI GCATC 4 cut(s) 977, 1006, 2069, 2093
MabI ACCWGGT 1 cut(s) 133
MaeI CTAG 5 cut(s) 660, 1311, 1989, 2021, 2168
MaeIII GTNAC 8 cut(s) 64, 137, 230, 649, 950, 1583, 1795, 2027
MalI GATC 8 cut(s) 302, 327, 367, 535, 729, 771, 1326, 1980
MboI GATC 8 cut(s) 300, 325, 365, 533, 727, 769, 1324, 1978
MflI RGATCY 3 cut(s) 727, 769, 1978
MhlI GDGCHC 6 cut(s) 41, 168, 1191, 1366, 1581, 1861
MlsI TGGCCA 2 cut(s) 560, 1278
MluNI TGGCCA 2 cut(s) 560, 1278
MlyI GAGTC 3 cut(s) 13, 112, 646
MmeI TCCRAC 2 cut(s) 680, 901
Mox20I TGGCCA 2 cut(s) 560, 1278
MscI TGGCCA 2 cut(s) 560, 1278
MseI TTAA 3 cut(s) 72, 1821, 2213
MslI CAYNNNNRTG 3 cut(s) 1374, 1679, 2036
Msp20I TGGCCA 2 cut(s) 560, 1278
MspI CCGG 8 cut(s) 235, 644, 1203, 1271, 1825, 2064, 2083, 2107
MspR9I CCNGG 7 cut(s) 135, 732, 1107, 1280, 2064, 2065, 2220
Mva1269I GAATGC 2 cut(s) 1035, 1562
MvaI CCWGG 5 cut(s) 135, 732, 1107, 1280, 2220
NciI CCSGG 2 cut(s) 2064, 2065
NcoI CCATGG 1 cut(s) 881
NdeII GATC 8 cut(s) 300, 325, 365, 533, 727, 769, 1324, 1978
NlaIV GGNNCC 9 cut(s) 40, 549, 706, 875, 1111, 1146, 1249, 1719, 1980
NmuCI GTSAC 6 cut(s) 64, 137, 230, 649, 1583, 2027
NsbI TGCGCA 1 cut(s) 2015
NspI RCATGY 5 cut(s) 1591, 1948, 2002, 2020, 2056
OliI CACNNNNGTG 1 cut(s) 1679
PaeI GCATGC 2 cut(s) 1948, 2020
PaeR7I CTCGAG 2 cut(s) 1190, 1530
PagI TCATGA 2 cut(s) 1549, 2092
PceI AGGCCT 3 cut(s) 77, 983, 1993
PciI ACATGT 1 cut(s) 2052
PciSI GCTCTTC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 1 cut(s) 1537
PctI GAATGC 2 cut(s) 1035, 1562
PfeI GAWTC 5 cut(s) 284, 785, 902, 1395, 1457
PflMI CCANNNNNTGG 2 cut(s) 674, 1634
PfoI TCCNGGA 1 cut(s) 730
PleI GAGTC 3 cut(s) 12, 112, 646
PpsI GAGTC 3 cut(s) 12, 112, 646
PscI ACATGT 1 cut(s) 2052
PshAI GACNNNNGTC 1 cut(s) 2122
Psp124BI GAGCTC 1 cut(s) 1191
Psp6I CCWGG 5 cut(s) 133, 730, 1105, 1278, 2218
PspEI GGTNACC 1 cut(s) 230
PspFI CCCAGC 1 cut(s) 501
PspGI CCWGG 5 cut(s) 133, 730, 1105, 1278, 2218
PspN4I GGNNCC 9 cut(s) 40, 549, 706, 875, 1111, 1146, 1249, 1719, 1980
PspPI GGNCC 5 cut(s) 111, 641, 1109, 1816, 2159
PspXI VCTCGAGB 2 cut(s) 1190, 1530
PstI CTGCAG 1 cut(s) 474
PstNI CAGNNNCTG 3 cut(s) 593, 695, 2011
PsuI RGATCY 3 cut(s) 727, 769, 1978
RsaI GTAC 3 cut(s) 706, 813, 2189
RsaNI GTAC 3 cut(s) 705, 812, 2188
RseI CAYNNNNRTG 3 cut(s) 1374, 1679, 2036
SacI GAGCTC 1 cut(s) 1191
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 3 cut(s) 72, 1821, 2213
Sau3AI GATC 8 cut(s) 300, 325, 365, 533, 727, 769, 1324, 1978
Sau96I GGNCC 5 cut(s) 111, 641, 1109, 1816, 2159
ScaI AGTACT 1 cut(s) 813
SchI GAGTC 3 cut(s) 13, 112, 646
ScrFI CCNGG 7 cut(s) 135, 732, 1107, 1280, 2064, 2065, 2220
SduI GDGCHC 6 cut(s) 41, 168, 1191, 1366, 1581, 1861
SexAI ACCWGGT 1 cut(s) 133
SfaNI GCATC 4 cut(s) 977, 1006, 2069, 2093
SfcI CTRYAG 2 cut(s) 470, 1518
Sfr274I CTCGAG 2 cut(s) 1190, 1530
SinI GGWCC 1 cut(s) 1816
SlaI CTCGAG 2 cut(s) 1190, 1530
SmaI CCCGGG 1 cut(s) 2065
SmiMI CAYNNNNRTG 3 cut(s) 1374, 1679, 2036
SmlI CTYRAG 4 cut(s) 405, 1190, 1530, 2204
SmoI CTYRAG 4 cut(s) 405, 1190, 1530, 2204
SpeI ACTAGT 1 cut(s) 659
SphI GCATGC 2 cut(s) 1948, 2020
SseBI AGGCCT 3 cut(s) 77, 983, 1993
SsiI CCGC 6 cut(s) 19, 386, 1975, 2157, 2162, 2254
SspMI CTAG 5 cut(s) 660, 1311, 1989, 2021, 2168
SstI GAGCTC 1 cut(s) 1191
StuI AGGCCT 3 cut(s) 77, 983, 1993
StyD4I CCNGG 7 cut(s) 133, 730, 1105, 1278, 2062, 2063, 2218
StyI CCWWGG 3 cut(s) 881, 978, 1166
TaaI ACNGT 5 cut(s) 70, 143, 824, 1089, 1583
TaqI TCGA 6 cut(s) 7, 637, 962, 1191, 1404, 1531
TatI WGTACW 1 cut(s) 811
TauI GCSGC 1 cut(s) 2160
TfiI GAWTC 5 cut(s) 284, 785, 902, 1395, 1457
Tru1I TTAA 3 cut(s) 72, 1821, 2213
Tru9I TTAA 3 cut(s) 72, 1821, 2213
TscAI CASTG 6 cut(s) 461, 479, 1066, 1092, 1635, 1855
TseFI GTSAC 6 cut(s) 64, 137, 230, 649, 1583, 2027
TseI GCWGC 9 cut(s) 469, 1526, 1660, 1837, 1948, 1955, 2131, 2134, 2137
Tsp45I GTSAC 6 cut(s) 64, 137, 230, 649, 1583, 2027
TspDTI ATGAA 7 cut(s) 330, 506, 739, 1538, 1566, 1905, 2250
TspGWI ACGGA 1 cut(s) 1240
TspMI CCCGGG 1 cut(s) 2063
TspRI CASTG 6 cut(s) 461, 479, 1066, 1092, 1635, 1855
Van91I CCANNNNNTGG 2 cut(s) 674, 1634
VneI GTGCAC 1 cut(s) 1577
VpaK11BI GGWCC 1 cut(s) 1816
XapI RAATTY 5 cut(s) 523, 893, 995, 1877, 2209
XceI RCATGY 5 cut(s) 1591, 1948, 2002, 2020, 2056
XhoI CTCGAG 2 cut(s) 1190, 1530
XmaI CCCGGG 1 cut(s) 2063
XmiI GTMKAC 1 cut(s) 1969
XspI CTAG 5 cut(s) 660, 1311, 1989, 2021, 2168
ZrmI AGTACT 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.