pycom09g11200
NAC Family

inactive protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001566
Physical Location & Seq
Reverse (-)
9380639 .. 9382519
1881 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g11200.6

Sequence Viewer

Length: 1368 bp
ATGGAAGACAGCTCACACAGAAGTAACGATAATTCAAAAGCATCTATTACCAAGGATTTGCTGAAATTTTTGAAACTTGATAAAGATGCTGGAATTGGAATGCGAAATTTTAGAGCTGACATGGAGTTCAGTGGAAATCTGAGAGAAGCAATTTCACTGTCTAGTAATGCTCCTCCAGGCCCCCCTCCATTATGTTCAATATGTCAACACAAGGCACCTGTTTTTGGAAAACCTCCAAGGTGGTTTAGCTATGCAGAGCTGGAGCTTGCTACTGGAGGATTTTCGCAAGCCAATTTTTTGGCCGAAGGAGGGTTTGGTTCTGTTCACAGAGGGGTGCTACCGGATGGCCAGGCTGTTGCTGTCAAGCAACATAAATTGGCAAGTTCTCAGGGGGATCAGGAGTTCTGTTCAGAAGTAGAAGTCCTGAGCTGTGCTCAGCACCGAAATGTTGTCATGTTGATTGGCTTCTGTATCGAGGACAAAAGAAGATTGCTGGTCTATGAATATATATGCAATGGGTCATTAGATTCTCATCTATACAGTAATATATCTTTCTCTGGCAGTGGGCGACATCGTGAGCCTTTAGAATGGTCTGCACGGCAAAAGATCGCTGTAGGGGCAGCTCGAGGGCTACGATATCTTCATGAAGAATGCAGAGTGGGATGCATTGTGCACCGTGACATGCGGCCAAACAACATCCTCATCACCCATGATTTTGAGCCACTGGTTGGGGATTTTGGCCTCGCAAGGTGGCAGCCTGATGGGGACACGGGTGTGGACACAAGAGTCATTGGAACATTCGGATATTTGGCTCCTGAATATGCTCAGAGTGGTCAAATCACAGAAAAGGCTGATGTTTATTCCTTCGGGGTGGTCTTGGTGGAACTTGTTACAGGGCGAAAAGCTGTGGACCTTAACCGGCCTAAGGGGCAGCAATGTCTCACTGAATGGGCGCGGCCATTATTAGAAGAATATGCCATTGATGAACTGATTGACCCAAGACTTGACAACTTCTATTCCGAACACGAGGTTTACTGCATGCTGCACGCTGCTTCGCTATGTATACGGCGGGATCCTCAATCTAGGCCTCGCATGTCTCAGGTTCTCCGTATACTAGAAGGTGACATGGTGATGGATACAAATTACATGTCAACGGCCGGGTACGATGTAGGATGCCGGAATGGTCACGATGTGGGATGCCGGAGTGGTCGACTTTGGTCAGAGCAGCAGCAGCAGAAGGAACATCATAGCGGCCCTCTATTTGACGAAGCGATGGACGGGTACGAGAAGCTCTCTCGTGAGAATTTAAGGCCAGGTTTCAGGGAGAGGGACAGGGTAAGGAGGACTCCATGTGAAAACCATTTGTAA

Protein Analysis

456

Amino Acids

51.17

Weight (kDa)

6.08

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 785
AccB1I GGYRCC 1 cut(s) 214
AccB7I CCANNNNNTGG 1 cut(s) 728
AccI GTMKAC 3 cut(s) 1063, 1111, 1210
AccII CGCG 1 cut(s) 955
AciI CCGC 4 cut(s) 685, 955, 1069, 1251
AclWI GGATC 3 cut(s) 402, 1067, 1080
AcoI YGGCCR 5 cut(s) 300, 346, 686, 956, 1155
AcsI RAATTY 3 cut(s) 65, 106, 1303
AdeI CACNNNGTG 1 cut(s) 1192
AfaI GTAC 2 cut(s) 1163, 1283
AfiI CCNNNNNNNGG 4 cut(s) 224, 309, 728, 925
AflIII ACRYGT 1 cut(s) 1146
AgsI TTSAA 3 cut(s) 36, 73, 198
AjnI CCWGG 3 cut(s) 175, 348, 1312
AjuI GAANNNNNNNTTGG 2 cut(s) 297, 329
AleI CACNNNNGTG 1 cut(s) 773
AloI GAACNNNNNNTCC 2 cut(s) 386, 418
AluBI AGCT 9 cut(s) 12, 116, 249, 259, 265, 429, 623, 905, 1291
AluI AGCT 9 cut(s) 12, 116, 249, 259, 265, 429, 623, 905, 1291
Alw21I GWGCWC 2 cut(s) 436, 675
Alw26I GTCTC 2 cut(s) 944, 1101
Alw44I GTGCAC 1 cut(s) 671
AlwI GGATC 3 cut(s) 402, 1067, 1080
Ama87I CYCGRG 1 cut(s) 624
ApaLI GTGCAC 1 cut(s) 671
ApeKI GCWGC 8 cut(s) 620, 754, 931, 1042, 1049, 1225, 1228, 1231
ApoI RAATTY 3 cut(s) 65, 106, 1303
ArsI GACNNNNNNTTYG 2 cut(s) 1133, 1165
AspLEI GCGC 1 cut(s) 955
AspS9I GGNCC 3 cut(s) 179, 910, 1253
AsuC2I CCSGG 1 cut(s) 1159
AsuHPI GGTGA 3 cut(s) 697, 1133, 1141
AvaI CYCGRG 1 cut(s) 624
AvaII GGWCC 1 cut(s) 910
AxyI CCTNAGG 1 cut(s) 924
BaeGI GKGCMC 1 cut(s) 675
BalI TGGCCA 1 cut(s) 348
BamHI GGATCC 1 cut(s) 1072
BanI GGYRCC 1 cut(s) 214
BauI CACGAG 2 cut(s) 1025, 1296
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 2 cut(s) 436, 675
BbvI GCAGC 8 cut(s) 632, 766, 943, 1029, 1036, 1237, 1240, 1243
BccI CCATC 4 cut(s) 338, 755, 1126, 1267
BceAI ACGGC 3 cut(s) 614, 1082, 1170
BciT130I CCWGG 3 cut(s) 177, 350, 1314
BciVI GTATCC 1 cut(s) 1129
BcnI CCSGG 1 cut(s) 1159
BcoDI GTCTC 2 cut(s) 944, 1101
BfaI CTAG 3 cut(s) 162, 1083, 1115
BfmI CTRYAG 1 cut(s) 612
BfuI GTATCC 1 cut(s) 1129
BglI GCCNNNNNGGC 1 cut(s) 928
BlpI GCTNAGC 1 cut(s) 435
Bme1390I CCNGG 4 cut(s) 177, 350, 1159, 1314
Bme18I GGWCC 1 cut(s) 910
BmeT110I CYCGRG 1 cut(s) 624
BmgT120I GGNCC 3 cut(s) 179, 910, 1253
BmiI GGNNCC 4 cut(s) 181, 216, 813, 1074
BmrFI CCNGG 4 cut(s) 177, 350, 1159, 1314
BmsI GCATC 5 cut(s) 50, 76, 653, 1163, 1187
BoxI GACNNNNGTC 1 cut(s) 1216
BpiI GAAGAC 1 cut(s) 12
BplI GAGNNNNNCTC 4 cut(s) 418, 450, 1277, 1309
BpmI CTGGAG 3 cut(s) 159, 281, 294
Bpu10I CCTNAGC 1 cut(s) 425
Bpu1102I GCTNAGC 1 cut(s) 435
BpuMI CCSGG 1 cut(s) 1159
BsaBI GATNNNNATC 1 cut(s) 531
BsaJI CCNNGG 2 cut(s) 51, 236
BsaWI WCCGGW 1 cut(s) 340
Bsc4I CCNNNNNNNGG 4 cut(s) 224, 309, 728, 925
Bse118I RCCGGY 1 cut(s) 918
Bse1I ACTGG 2 cut(s) 277, 729
Bse21I CCTNAGG 1 cut(s) 924
Bse3DI GCAATG 2 cut(s) 520, 941
Bse8I GATNNNNATC 1 cut(s) 531
BseBI CCWGG 3 cut(s) 177, 350, 1314
BseDI CCNNGG 2 cut(s) 51, 236
BseGI GGATG 5 cut(s) 349, 668, 696, 1178, 1202
BseJI GATNNNNATC 1 cut(s) 531
BseLI CCNNNNNNNGG 4 cut(s) 224, 309, 728, 925
BseMI GCAATG 2 cut(s) 520, 941
BseMII CTCAG 6 cut(s) 131, 401, 416, 449, 839, 1112
BseNI ACTGG 2 cut(s) 277, 729
BseRI GAGGAG 1 cut(s) 162
BseSI GKGCMC 1 cut(s) 675
BseX3I CGGCCG 1 cut(s) 1155
BseXI GCAGC 8 cut(s) 632, 766, 943, 1029, 1036, 1237, 1240, 1243
BsgI GTGCAG 2 cut(s) 579, 1028
Bsh1236I CGCG 1 cut(s) 955
Bsh1285I CGRYCG 1 cut(s) 1158
BshNI GGYRCC 1 cut(s) 214
BsiEI CGRYCG 1 cut(s) 1158
BsiHKAI GWGCWC 2 cut(s) 436, 675
BsiHKCI CYCGRG 1 cut(s) 624
BsiSI CCGG 5 cut(s) 341, 919, 1158, 1177, 1201
BslFI GGGAC 2 cut(s) 779, 1343
BslI CCNNNNNNNGG 4 cut(s) 224, 309, 728, 925
BsmAI GTCTC 2 cut(s) 944, 1101
BsmFI GGGAC 2 cut(s) 779, 1343
BsmI GAATGC 2 cut(s) 105, 656
BsoBI CYCGRG 1 cut(s) 624
Bsp1286I GDGCHC 2 cut(s) 436, 675
Bsp143I GATC 3 cut(s) 394, 606, 1072
Bsp1720I GCTNAGC 1 cut(s) 435
BspACI CCGC 4 cut(s) 685, 955, 1069, 1251
BspCNI CTCAG 6 cut(s) 132, 400, 417, 448, 838, 1111
BspFNI CGCG 1 cut(s) 955
BspHI TCATGA 1 cut(s) 643
BspLI GGNNCC 4 cut(s) 181, 216, 813, 1074
BspPI GGATC 3 cut(s) 402, 1067, 1080
BspT107I GGYRCC 1 cut(s) 214
BsrDI GCAATG 2 cut(s) 520, 941
BsrFI RCCGGY 1 cut(s) 918
BsrI ACTGG 2 cut(s) 277, 729
BssAI RCCGGY 1 cut(s) 918
BssECI CCNNGG 2 cut(s) 51, 236
BssMI GATC 3 cut(s) 394, 606, 1072
BssNAI GTATAC 2 cut(s) 1064, 1112
BssSI CACGAG 2 cut(s) 1025, 1296
BssT1I CCWWGG 2 cut(s) 51, 236
Bst1107I GTATAC 2 cut(s) 1064, 1112
Bst2BI CACGAG 2 cut(s) 1025, 1296
Bst2UI CCWGG 3 cut(s) 177, 350, 1314
Bst4CI ACNGT 3 cut(s) 159, 542, 677
BstC8I GCNNGC 4 cut(s) 267, 288, 1040, 1047
BstDEI CTNAG 7 cut(s) 140, 387, 425, 435, 825, 924, 1098
BstF5I GGATG 5 cut(s) 349, 668, 696, 1178, 1202
BstFNI CGCG 1 cut(s) 955
BstHHI GCGC 1 cut(s) 955
BstKTI GATC 3 cut(s) 397, 609, 1075
BstMAI GTCTC 2 cut(s) 944, 1101
BstMBI GATC 3 cut(s) 394, 606, 1072
BstMCI CGRYCG 1 cut(s) 1158
BstMWI GCNNNNNNNGC 3 cut(s) 617, 928, 1231
BstNI CCWGG 3 cut(s) 177, 350, 1314
BstNSI RCATGY 4 cut(s) 685, 1042, 1096, 1150
BstPAI GACNNNNGTC 1 cut(s) 1216
BstSCI CCNGG 4 cut(s) 175, 348, 1157, 1312
BstSFI CTRYAG 1 cut(s) 612
BstSLI GKGCMC 1 cut(s) 675
BstUI CGCG 1 cut(s) 955
BstV1I GCAGC 8 cut(s) 632, 766, 943, 1029, 1036, 1237, 1240, 1243
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 1072
BstXI CCANNNNNNTGG 1 cut(s) 298
BstYI RGATCY 1 cut(s) 1072
BstZ17I GTATAC 2 cut(s) 1064, 1112
BstZI CGGCCG 1 cut(s) 1155
Bsu36I CCTNAGG 1 cut(s) 924
BsuI GTATCC 1 cut(s) 1129
BtgZI GCGATG 1 cut(s) 1286
BtsCI GGATG 5 cut(s) 349, 668, 696, 1178, 1202
BtsI GCAGTG 1 cut(s) 568
BtsIMutI CAGTG 5 cut(s) 136, 155, 568, 722, 942
Cac8I GCNNGC 4 cut(s) 267, 288, 1040, 1047
CciI TCATGA 1 cut(s) 643
CfoI GCGC 1 cut(s) 955
Cfr10I RCCGGY 1 cut(s) 918
Cfr13I GGNCC 3 cut(s) 179, 910, 1253
Csp6I GTAC 2 cut(s) 1162, 1282
CviQI GTAC 2 cut(s) 1162, 1282
DdeI CTNAG 7 cut(s) 140, 387, 425, 435, 825, 924, 1098
DpnI GATC 3 cut(s) 396, 608, 1074
DpnII GATC 3 cut(s) 394, 606, 1072
DraIII CACNNNGTG 1 cut(s) 1192
DrdI GACNNNNNNGTC 1 cut(s) 785
DseDI GACNNNNNNGTC 1 cut(s) 785
EaeI YGGCCR 5 cut(s) 300, 346, 686, 956, 1155
EagI CGGCCG 1 cut(s) 1155
EclXI CGGCCG 1 cut(s) 1155
Eco130I CCWWGG 2 cut(s) 51, 236
Eco147I AGGCCT 1 cut(s) 1087
Eco32I GATATC 1 cut(s) 638
Eco47I GGWCC 1 cut(s) 910
Eco52I CGGCCG 1 cut(s) 1155
Eco81I CCTNAGG 1 cut(s) 924
Eco88I CYCGRG 1 cut(s) 624
EcoO109I RGGNCCY 1 cut(s) 179
EcoRII CCWGG 3 cut(s) 175, 348, 1312
EcoRV GATATC 1 cut(s) 638
EcoT14I CCWWGG 2 cut(s) 51, 236
EcoT22I ATGCAT 1 cut(s) 668
ErhI CCWWGG 2 cut(s) 51, 236
FaqI GGGAC 2 cut(s) 779, 1343
FauI CCCGC 1 cut(s) 1062
FblI GTMKAC 3 cut(s) 1063, 1111, 1210
FokI GGATG 5 cut(s) 356, 675, 683, 1185, 1209
FspBI CTAG 3 cut(s) 162, 1083, 1115
GlaI GCGC 1 cut(s) 954
GsuI CTGGAG 3 cut(s) 159, 281, 294
HapII CCGG 5 cut(s) 341, 919, 1158, 1177, 1201
HhaI GCGC 1 cut(s) 955
Hin6I GCGC 1 cut(s) 953
HinP1I GCGC 1 cut(s) 953
HincII GTYRAC 3 cut(s) 206, 1152, 1211
HindII GTYRAC 3 cut(s) 206, 1152, 1211
HinfI GANTC 3 cut(s) 527, 786, 1345
HpaII CCGG 5 cut(s) 341, 919, 1158, 1177, 1201
HphI GGTGA 3 cut(s) 697, 1133, 1141
Hpy188I TCNGA 6 cut(s) 141, 412, 803, 828, 1021, 1222
Hpy188III TCNNGA 7 cut(s) 398, 424, 575, 644, 815, 1187, 1298
HpyAV CCTTC 4 cut(s) 299, 874, 1112, 1231
HpyCH4III ACNGT 3 cut(s) 159, 542, 677
HpyCH4V TGCA 8 cut(s) 254, 513, 596, 654, 666, 673, 1038, 1045
HpyF10VI GCNNNNNNNGC 3 cut(s) 617, 928, 1231
HpyF3I CTNAG 7 cut(s) 140, 387, 425, 435, 825, 924, 1098
HspAI GCGC 1 cut(s) 953
Kzo9I GATC 3 cut(s) 394, 606, 1072
LmnI GCTCC 3 cut(s) 175, 262, 817
Lsp1109I GCAGC 8 cut(s) 632, 766, 943, 1029, 1036, 1237, 1240, 1243
LweI GCATC 5 cut(s) 50, 76, 653, 1163, 1187
MaeI CTAG 3 cut(s) 162, 1083, 1115
MaeIII GTNAC 5 cut(s) 23, 677, 889, 1121, 1184
MalI GATC 3 cut(s) 396, 608, 1074
MboI GATC 3 cut(s) 394, 606, 1072
MboII GAAGA 5 cut(s) 17, 498, 632, 659, 980
MflI RGATCY 1 cut(s) 1072
MhlI GDGCHC 2 cut(s) 436, 675
MlsI TGGCCA 1 cut(s) 348
MluCI AATT 9 cut(s) 31, 65, 93, 106, 150, 292, 374, 1141, 1303
MluNI TGGCCA 1 cut(s) 348
MlyI GAGTC 2 cut(s) 795, 1339
Mox20I TGGCCA 1 cut(s) 348
Mph1103I ATGCAT 1 cut(s) 668
MscI TGGCCA 1 cut(s) 348
MseI TTAA 2 cut(s) 915, 1307
MslI CAYNNNNRTG 3 cut(s) 444, 773, 1130
Msp20I TGGCCA 1 cut(s) 348
MspI CCGG 5 cut(s) 341, 919, 1158, 1177, 1201
MspR9I CCNGG 4 cut(s) 177, 350, 1159, 1314
Mva1269I GAATGC 2 cut(s) 105, 656
MvaI CCWGG 3 cut(s) 177, 350, 1314
MvnI CGCG 1 cut(s) 955
MwoI GCNNNNNNNGC 3 cut(s) 617, 928, 1231
NciI CCSGG 1 cut(s) 1159
NdeII GATC 3 cut(s) 394, 606, 1072
NlaIV GGNNCC 4 cut(s) 181, 216, 813, 1074
NmuCI GTSAC 3 cut(s) 677, 1121, 1184
NsiI ATGCAT 1 cut(s) 668
NspI RCATGY 4 cut(s) 685, 1042, 1096, 1150
OliI CACNNNNGTG 1 cut(s) 773
PaeI GCATGC 1 cut(s) 1042
PaeR7I CTCGAG 1 cut(s) 624
PagI TCATGA 1 cut(s) 643
PceI AGGCCT 1 cut(s) 1087
PciI ACATGT 1 cut(s) 1146
PcsI WCGNNNNNNNCGW 1 cut(s) 631
PctI GAATGC 2 cut(s) 105, 656
PfeI GAWTC 1 cut(s) 527
PflMI CCANNNNNTGG 1 cut(s) 728
PleI GAGTC 2 cut(s) 794, 1339
PpsI GAGTC 2 cut(s) 794, 1339
PscI ACATGT 1 cut(s) 1146
PshAI GACNNNNGTC 1 cut(s) 1216
Psp6I CCWGG 3 cut(s) 175, 348, 1312
PspGI CCWGG 3 cut(s) 175, 348, 1312
PspN4I GGNNCC 4 cut(s) 181, 216, 813, 1074
PspPI GGNCC 3 cut(s) 179, 910, 1253
PspXI VCTCGAGB 1 cut(s) 624
PsuI RGATCY 1 cut(s) 1072
RsaI GTAC 2 cut(s) 1163, 1283
RsaNI GTAC 2 cut(s) 1162, 1282
RseI CAYNNNNRTG 3 cut(s) 444, 773, 1130
SalI GTCGAC 1 cut(s) 1209
SaqAI TTAA 2 cut(s) 915, 1307
Sau3AI GATC 3 cut(s) 394, 606, 1072
Sau96I GGNCC 3 cut(s) 179, 910, 1253
SchI GAGTC 2 cut(s) 795, 1339
ScrFI CCNGG 4 cut(s) 177, 350, 1159, 1314
SduI GDGCHC 2 cut(s) 436, 675
SfaNI GCATC 5 cut(s) 50, 76, 653, 1163, 1187
SfcI CTRYAG 1 cut(s) 612
Sfr274I CTCGAG 1 cut(s) 624
SinI GGWCC 1 cut(s) 910
SlaI CTCGAG 1 cut(s) 624
SmiMI CAYNNNNRTG 3 cut(s) 444, 773, 1130
SmlI CTYRAG 1 cut(s) 624
SmoI CTYRAG 1 cut(s) 624
SphI GCATGC 1 cut(s) 1042
Sse9I AATT 9 cut(s) 31, 65, 93, 106, 150, 292, 374, 1141, 1303
SseBI AGGCCT 1 cut(s) 1087
SsiI CCGC 4 cut(s) 685, 955, 1069, 1251
SspMI CTAG 3 cut(s) 162, 1083, 1115
StuI AGGCCT 1 cut(s) 1087
StyD4I CCNGG 4 cut(s) 175, 348, 1157, 1312
StyI CCWWGG 2 cut(s) 51, 236
TaaI ACNGT 3 cut(s) 159, 542, 677
TaqI TCGA 3 cut(s) 474, 625, 1210
TasI AATT 9 cut(s) 31, 65, 93, 106, 150, 292, 374, 1141, 1303
TauI GCSGC 3 cut(s) 688, 958, 1254
TfiI GAWTC 1 cut(s) 527
Tru1I TTAA 2 cut(s) 915, 1307
Tru9I TTAA 2 cut(s) 915, 1307
TscAI CASTG 5 cut(s) 136, 162, 568, 729, 949
TseFI GTSAC 3 cut(s) 677, 1121, 1184
TseI GCWGC 8 cut(s) 620, 754, 931, 1042, 1049, 1225, 1228, 1231
Tsp45I GTSAC 3 cut(s) 677, 1121, 1184
TspDTI ATGAA 4 cut(s) 516, 632, 660, 999
TspGWI ACGGA 1 cut(s) 1097
TspRI CASTG 5 cut(s) 136, 162, 568, 729, 949
Van91I CCANNNNNTGG 1 cut(s) 728
VneI GTGCAC 1 cut(s) 671
VpaK11BI GGWCC 1 cut(s) 910
XapI RAATTY 3 cut(s) 65, 106, 1303
XceI RCATGY 4 cut(s) 685, 1042, 1096, 1150
XhoI CTCGAG 1 cut(s) 624
XmiI GTMKAC 3 cut(s) 1063, 1111, 1210
XspI CTAG 3 cut(s) 162, 1083, 1115
Zsp2I ATGCAT 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.