Rorug02G0375200
NAC Family

inactive protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001566
Physical Location & Seq
Forward (+)
47814377 .. 47816349
1973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0375200.1

Sequence Viewer

Length: 840 bp
ATGAACGGCACCGTTTCGGCCCTCACTTCTCGGCGCCACAAACGAGCCGCCGTCGCCGCCACAAACGGCCCCACCCCTACCGCCGCCATGTCCAAGAAGCTCCTCCCCGTCCACGGCTGCGGCGGCAACAAGGCCTCCTTCATGACGTGGACGGTGCATGACGCCGTCCACGTCGCCAAGCACCACTGGATACCCTGCTTCTTCGCAATGGGCCTCCTCTTCTTCATGGGCGTCGAGTACACGCTCCGCATGGTTCCCTCCTCCGCGCCGCCGTTCGATATCGGCTTCGTCACCACGCGCTCCCTCCACCTCCTCCTGGCCTCGAGCCCCCAGCTCAACACATTGCTCGCTGGCCTCAACACGGTGTTTGTGGGAATGCAGACAACGTATATATTGTGGACGTGGCTAATCGAGGGCCGTCCGAGAGCCACCATCGCTGCCCTATTTATGTTCACTTGCCGGGGAATCCTTGGCTACTCCACCCAACTTCCATTGCCTCAGGGATTTTTGGGGTCGGGAGCAGACTTTCCGGTTGGGAATGTATCGTTTTTCTTGTTCTACTCCGGGCATGTAGCAGGGTCTGTGATTGCATCCCTCGACATGAGAAGAATGCAGAGGTGGGAATTGGCATGGACATTTGATGCATTAAATGTGTTGCAAGCTGTGAGATTGTTAGGGACTAGAGGACACTACACAATAGACTTGGCGGTTGGGGTTGGGGCCGGTATTCTCTTTGATTCTCTGGCAGGGAAGTACGAAGAAAGCAAGAGATTAAATTCAAAAGTAGCTTCAACTACTAGTAATGGTGCCAAAGAGGCTTTGTTTCCATTAGCAGATTAG

Protein Analysis

279

Amino Acids

30.24

Weight (kDa)

9.75

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AtPDCT1_2 PF24788 92 - 251 8.2e-92 AtPDCT1/2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 8, 33, 806
AccII CGCG 2 cut(s) 266, 298
AcsI RAATTY 1 cut(s) 775
AcyI GRCGYC 3 cut(s) 34, 162, 231
AfaI GTAC 2 cut(s) 239, 755
AfiI CCNNNNNNNGG 3 cut(s) 113, 316, 361
AgsI TTSAA 2 cut(s) 780, 792
AhlI ACTAGT 1 cut(s) 797
AjiI CACGTC 3 cut(s) 147, 172, 402
AjnI CCWGG 1 cut(s) 315
AluBI AGCT 4 cut(s) 100, 334, 662, 788
AluI AGCT 4 cut(s) 100, 334, 662, 788
AlwNI CAGNNNCTG 1 cut(s) 581
Ama87I CYCGRG 1 cut(s) 322
AoxI GGCC 8 cut(s) 18, 67, 132, 211, 318, 352, 415, 720
ApeKI GCWGC 2 cut(s) 117, 437
ApoI RAATTY 1 cut(s) 775
AspLEI GCGC 3 cut(s) 36, 268, 300
AspS9I GGNCC 5 cut(s) 19, 68, 211, 415, 720
AsuC2I CCSGG 2 cut(s) 461, 565
AsuHPI GGTGA 1 cut(s) 283
AvaI CYCGRG 1 cut(s) 322
AxyI CCTNAGG 1 cut(s) 498
BanI GGYRCC 3 cut(s) 8, 33, 806
BanII GRGCYC 1 cut(s) 329
BbvI GCAGC 2 cut(s) 104, 424
BccI CCATC 1 cut(s) 440
BceAI ACGGC 7 cut(s) 22, 35, 82, 130, 149, 256, 402
BciT130I CCWGG 1 cut(s) 317
BciVI GTATCC 1 cut(s) 183
BcnI CCSGG 2 cut(s) 461, 565
BcuI ACTAGT 1 cut(s) 797
BfaI CTAG 2 cut(s) 681, 798
BfoI RGCGCY 1 cut(s) 37
BfuI GTATCC 1 cut(s) 183
BglI GCCNNNNNGGC 1 cut(s) 815
BisI GCNGC 8 cut(s) 48, 57, 84, 118, 121, 124, 269, 438
BlsI GCNGC 8 cut(s) 49, 58, 85, 119, 122, 125, 270, 439
Bme1390I CCNGG 3 cut(s) 317, 461, 565
BmeT110I CYCGRG 1 cut(s) 322
BmgBI CACGTC 3 cut(s) 147, 172, 402
BmgT120I GGNCC 5 cut(s) 19, 68, 211, 415, 720
BmiI GGNNCC 6 cut(s) 10, 35, 70, 255, 721, 808
BmrFI CCNGG 3 cut(s) 317, 461, 565
BmsI GCATC 2 cut(s) 599, 631
BpuMI CCSGG 2 cut(s) 461, 565
BsaHI GRCGYC 3 cut(s) 34, 162, 231
BsaJI CCNNGG 3 cut(s) 112, 460, 469
BsaWI WCCGGW 1 cut(s) 529
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 316, 361
Bse118I RCCGGY 1 cut(s) 722
Bse1I ACTGG 1 cut(s) 191
Bse21I CCTNAGG 1 cut(s) 498
Bse3DI GCAATG 3 cut(s) 213, 341, 491
BseBI CCWGG 1 cut(s) 317
BseDI CCNNGG 3 cut(s) 112, 460, 469
BseGI GGATG 1 cut(s) 590
BseLI CCNNNNNNNGG 3 cut(s) 113, 316, 361
BseMI GCAATG 3 cut(s) 213, 341, 491
BseMII CTCAG 1 cut(s) 512
BseNI ACTGG 1 cut(s) 191
BseRI GAGGAG 4 cut(s) 92, 206, 250, 302
BseXI GCAGC 2 cut(s) 104, 424
BseYI CCCAGC 1 cut(s) 330
Bsh1236I CGCG 2 cut(s) 266, 298
BshFI GGCC 8 cut(s) 20, 69, 134, 213, 320, 354, 417, 722
BshNI GGYRCC 3 cut(s) 8, 33, 806
BsiHKCI CYCGRG 1 cut(s) 322
BsiSI CCGG 4 cut(s) 460, 530, 564, 723
BslFI GGGAC 1 cut(s) 691
BslI CCNNNNNNNGG 3 cut(s) 113, 316, 361
BsmFI GGGAC 1 cut(s) 691
BsmI GAATGC 2 cut(s) 381, 615
BsnI GGCC 8 cut(s) 20, 69, 134, 213, 320, 354, 417, 722
BsoBI CYCGRG 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 329
BspANI GGCC 8 cut(s) 20, 69, 134, 213, 320, 354, 417, 722
BspCNI CTCAG 1 cut(s) 511
BspFNI CGCG 2 cut(s) 266, 298
BspHI TCATGA 1 cut(s) 141
BspLI GGNNCC 6 cut(s) 10, 35, 70, 255, 721, 808
BspT107I GGYRCC 3 cut(s) 8, 33, 806
BsrDI GCAATG 3 cut(s) 213, 341, 491
BsrFI RCCGGY 1 cut(s) 722
BsrI ACTGG 1 cut(s) 191
BssAI RCCGGY 1 cut(s) 722
BssECI CCNNGG 3 cut(s) 112, 460, 469
BssNI GRCGYC 3 cut(s) 34, 162, 231
BssT1I CCWWGG 1 cut(s) 469
Bst2UI CCWGG 1 cut(s) 317
Bst4CI ACNGT 3 cut(s) 13, 154, 364
Bst6I CTCTTC 1 cut(s) 224
BstACI GRCGYC 3 cut(s) 34, 162, 231
BstC8I GCNNGC 3 cut(s) 348, 352, 660
BstDEI CTNAG 1 cut(s) 498
BstDSI CCRYGG 1 cut(s) 112
BstF5I GGATG 1 cut(s) 590
BstFNI CGCG 2 cut(s) 266, 298
BstH2I RGCGCY 1 cut(s) 37
BstHHI GCGC 3 cut(s) 36, 268, 300
BstMWI GCNNNNNNNGC 5 cut(s) 53, 56, 123, 434, 815
BstNI CCWGG 1 cut(s) 317
BstNSI RCATGY 1 cut(s) 572
BstSCI CCNGG 3 cut(s) 315, 459, 563
BstUI CGCG 2 cut(s) 266, 298
BstV1I GCAGC 2 cut(s) 104, 424
Bsu36I CCTNAGG 1 cut(s) 498
BsuI GTATCC 1 cut(s) 183
BsuRI GGCC 8 cut(s) 20, 69, 134, 213, 320, 354, 417, 722
BtgI CCRYGG 1 cut(s) 112
BtgZI GCGATG 1 cut(s) 418
BtrI CACGTC 3 cut(s) 147, 172, 402
BtsCI GGATG 1 cut(s) 590
BtsIMutI CAGTG 1 cut(s) 184
Cac8I GCNNGC 3 cut(s) 348, 352, 660
CaiI CAGNNNCTG 1 cut(s) 581
CciI TCATGA 1 cut(s) 141
CfoI GCGC 3 cut(s) 36, 268, 300
Cfr10I RCCGGY 1 cut(s) 722
Cfr13I GGNCC 5 cut(s) 19, 68, 211, 415, 720
CseI GACGC 2 cut(s) 170, 220
Csp6I GTAC 2 cut(s) 238, 754
CviAII CATG 8 cut(s) 88, 142, 158, 226, 250, 569, 601, 630
CviQI GTAC 2 cut(s) 238, 754
DdeI CTNAG 1 cut(s) 498
DinI GGCGCC 1 cut(s) 35
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
Eco130I CCWWGG 1 cut(s) 469
Eco147I AGGCCT 1 cut(s) 134
Eco24I GRGCYC 1 cut(s) 329
Eco32I GATATC 1 cut(s) 280
Eco81I CCTNAGG 1 cut(s) 498
Eco88I CYCGRG 1 cut(s) 322
EcoRII CCWGG 1 cut(s) 315
EcoRV GATATC 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 469
EcoT22I ATGCAT 1 cut(s) 646
EcoT38I GRGCYC 1 cut(s) 329
EgeI GGCGCC 1 cut(s) 35
EheI GGCGCC 1 cut(s) 35
ErhI CCWWGG 1 cut(s) 469
FaeI CATG 8 cut(s) 91, 145, 161, 229, 253, 572, 604, 633
FalI AAGNNNNNCTT 2 cut(s) 122, 154
FaqI GGGAC 1 cut(s) 691
FatI CATG 8 cut(s) 87, 141, 157, 225, 249, 568, 600, 629
Fnu4HI GCNGC 8 cut(s) 48, 57, 84, 118, 121, 124, 269, 438
FokI GGATG 1 cut(s) 577
FriOI GRGCYC 1 cut(s) 329
Fsp4HI GCNGC 8 cut(s) 48, 57, 84, 118, 121, 124, 269, 438
FspBI CTAG 2 cut(s) 681, 798
GlaI GCGC 3 cut(s) 35, 267, 299
GluI GCNGC 8 cut(s) 48, 57, 84, 118, 121, 124, 269, 438
GsaI CCCAGC 1 cut(s) 334
HaeII RGCGCY 1 cut(s) 37
HaeIII GGCC 8 cut(s) 20, 69, 134, 213, 320, 354, 417, 722
HapII CCGG 4 cut(s) 460, 530, 564, 723
HgaI GACGC 2 cut(s) 170, 220
HhaI GCGC 3 cut(s) 36, 268, 300
Hin1I GRCGYC 3 cut(s) 34, 162, 231
Hin1II CATG 8 cut(s) 91, 145, 161, 229, 253, 572, 604, 633
Hin6I GCGC 3 cut(s) 34, 266, 298
HinP1I GCGC 3 cut(s) 34, 266, 298
HinfI GANTC 2 cut(s) 465, 737
HpaII CCGG 4 cut(s) 460, 530, 564, 723
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 6 cut(s) 112, 150, 169, 240, 399, 453
Hpy188I TCNGA 1 cut(s) 423
Hpy188III TCNNGA 2 cut(s) 142, 516
Hpy8I GTNNAC 6 cut(s) 112, 150, 169, 240, 399, 453
Hpy99I CGWCG 3 cut(s) 56, 176, 236
HpyAV CCTTC 1 cut(s) 148
HpyCH4III ACNGT 3 cut(s) 13, 154, 364
HpyCH4IV ACGT 4 cut(s) 146, 171, 386, 401
HpyCH4V TGCA 6 cut(s) 157, 379, 590, 613, 644, 658
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 56, 123, 434, 815
HpyF3I CTNAG 1 cut(s) 498
HpySE526I ACGT 4 cut(s) 146, 171, 386, 401
Hsp92I GRCGYC 3 cut(s) 34, 162, 231
Hsp92II CATG 8 cut(s) 91, 145, 161, 229, 253, 572, 604, 633
HspAI GCGC 3 cut(s) 34, 266, 298
KasI GGCGCC 1 cut(s) 33
LmnI GCTCC 4 cut(s) 105, 249, 305, 518
Lsp1109I GCAGC 2 cut(s) 104, 424
LweI GCATC 2 cut(s) 599, 631
MaeI CTAG 2 cut(s) 681, 798
MaeII ACGT 4 cut(s) 146, 171, 386, 401
MaeIII GTNAC 1 cut(s) 289
MboII GAAGA 5 cut(s) 193, 211, 214, 618, 770
MhlI GDGCHC 1 cut(s) 329
MluCI AATT 2 cut(s) 623, 775
Mly113I GGCGCC 1 cut(s) 34
Mph1103I ATGCAT 1 cut(s) 646
MseI TTAA 2 cut(s) 647, 773
MspI CCGG 4 cut(s) 460, 530, 564, 723
MspR9I CCNGG 3 cut(s) 317, 461, 565
Mva1269I GAATGC 2 cut(s) 381, 615
MvaI CCWGG 1 cut(s) 317
MvnI CGCG 2 cut(s) 266, 298
MwoI GCNNNNNNNGC 5 cut(s) 53, 56, 123, 434, 815
NarI GGCGCC 1 cut(s) 34
NciI CCSGG 2 cut(s) 461, 565
NlaIII CATG 8 cut(s) 91, 145, 161, 229, 253, 572, 604, 633
NlaIV GGNNCC 6 cut(s) 10, 35, 70, 255, 721, 808
NmeAIII GCCGAG 1 cut(s) 10
NmuCI GTSAC 1 cut(s) 289
NsiI ATGCAT 1 cut(s) 646
NspI RCATGY 1 cut(s) 572
PaeR7I CTCGAG 1 cut(s) 322
PagI TCATGA 1 cut(s) 141
PceI AGGCCT 1 cut(s) 134
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PctI GAATGC 2 cut(s) 381, 615
PfeI GAWTC 2 cut(s) 465, 737
PflFI GACNNNGTC 1 cut(s) 164
PkrI GCNGC 8 cut(s) 49, 58, 85, 119, 122, 125, 270, 439
PluTI GGCGCC 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 315
PspFI CCCAGC 1 cut(s) 330
PspGI CCWGG 1 cut(s) 315
PspN4I GGNNCC 6 cut(s) 10, 35, 70, 255, 721, 808
PspPI GGNCC 5 cut(s) 19, 68, 211, 415, 720
PspXI VCTCGAGB 1 cut(s) 322
PstNI CAGNNNCTG 1 cut(s) 581
PsyI GACNNNGTC 1 cut(s) 164
RsaI GTAC 2 cut(s) 239, 755
RsaNI GTAC 2 cut(s) 238, 754
SaqAI TTAA 2 cut(s) 647, 773
SatI GCNGC 8 cut(s) 48, 57, 84, 118, 121, 124, 269, 438
Sau96I GGNCC 5 cut(s) 19, 68, 211, 415, 720
ScrFI CCNGG 3 cut(s) 317, 461, 565
SduI GDGCHC 1 cut(s) 329
SfaNI GCATC 2 cut(s) 599, 631
SfoI GGCGCC 1 cut(s) 35
Sfr274I CTCGAG 1 cut(s) 322
SlaI CTCGAG 1 cut(s) 322
SmlI CTYRAG 1 cut(s) 322
SmoI CTYRAG 1 cut(s) 322
SpeI ACTAGT 1 cut(s) 797
Sse9I AATT 2 cut(s) 623, 775
SseBI AGGCCT 1 cut(s) 134
SspDI GGCGCC 1 cut(s) 33
SspMI CTAG 2 cut(s) 681, 798
StuI AGGCCT 1 cut(s) 134
StyD4I CCNGG 3 cut(s) 315, 459, 563
StyI CCWWGG 1 cut(s) 469
TaaI ACNGT 3 cut(s) 13, 154, 364
TaiI ACGT 4 cut(s) 149, 174, 389, 404
TaqI TCGA 5 cut(s) 234, 276, 323, 411, 597
TasI AATT 2 cut(s) 623, 775
TatI WGTACW 1 cut(s) 237
TauI GCSGC 6 cut(s) 50, 59, 86, 123, 126, 271
TfiI GAWTC 2 cut(s) 465, 737
Tru1I TTAA 2 cut(s) 647, 773
Tru9I TTAA 2 cut(s) 647, 773
TscAI CASTG 1 cut(s) 191
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 2 cut(s) 117, 437
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 3 cut(s) 17, 130, 214
TspRI CASTG 1 cut(s) 191
Tth111I GACNNNGTC 1 cut(s) 164
XapI RAATTY 1 cut(s) 775
XceI RCATGY 1 cut(s) 572
XhoI CTCGAG 1 cut(s) 322
XspI CTAG 2 cut(s) 681, 798
Zsp2I ATGCAT 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.