Prupe.3G051500_v2.0.a1
NAC Family

inactive protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001566
Physical Location & Seq
Reverse (-)
3620885 .. 3627281
6397 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G051500.1

Sequence Viewer

Length: 2316 bp
ATGAGTCGAGAACAGAAGCGGGTGAGGCAAGAGAAAGGCTCCGATGACGCCGAGAAGGTTGTCGTGGCCGTTAAGGCGTCCAAGGAAATTCCCAAGACTGCTCTGGTGTGGGCCTTGACTCATGTTGTTCAACCTGGAGATTGCATAACACTGCTTGTGGTTGTCCCCTCTCAAAGTTCAGGTAGAAAATTTTGGGGCTTCCCAAGATTTGCAGGGGACTGTGCCAGTGGTAACCGGAAGTCTCATTCAGGAACAACCTCAGAGCTCAAGTGTGATATTTCAGATACCTGCTCTCAGATGATCCTTCAGCTGCATGAAGTTTATGATCCAAATAAGATAAATGTCAAGATAAAGATTATTTCTGGCTCACCTTCTGGTTCTGTGGCTGTAGAGGCCAAGAAAGCTCAAGCCAGTTGGGTTGTTTTAGACAAGCATCTTAAACATGAGGAAAAACACTGCATGGAAGAGCTGCAGTGCAACATTGTGGTTATGAAGCGTTCCCAGCCAAAAGTTCTTCGCTTGAATTTGAATGGATCTTCTAAGAAGGAACCTGAATTGGCTCGTTCCTTGCCTTCTCAGCTTGATGAAGGGACTGATAAACATCCCAAGAAGAAGAATGACTCTTTGAATTCCATTCGAGGGCCTGTTGTGACTCCAACTAGTAGTCCAGAGCTAGGGACTCCATTTACAGCCACTGAGGCTGGAACTTCATCGGTGTCAAGTTCAGATCCAGGAACTTCACCATTTTTCGTTTCAGAAATAAATGGAGACATGAAGAAAGAGGAATCATTAGTTAGTAAAGAAAACAAGGTTCTTGATGACAGTAGTTCAGACACTGACAGTGAAAACTTATCAACATCTTCAGCAAGTATGAGGTTCCAACCATGGATAGCAGAATTTCTTAATTCTCATCGTCCATCCTCGCAACACATGGAAGAAAGTTCACACAGAACTAATGATAATTCTAAAGCATCCACTACCAAAGCTTTGCTAGAGAAATTTTCGAAACTCGATAAGGATGCTGGAATTGGAATGCCAAATTATAGAGCTGACATGGAGTTCAGTGGAAATCTGAGAGAAGCAATTTCTCTGTCTAGAAATGCTCCTCCAGTCCCTCCTCCATTGTGTTCAATATGTCAACACAAGGCACCTGTGTTTGGAAAACCTCCAAGGTGGTTCAGCTATGCGGAGCTGGAGCTTGCTACTGGAGGATTTTCTCAAGCCAATTTTTTGGCTGAAGGTGGGTTTGGCTCTGTTCATAGAGGGGTGCTACCAGATGGCCAGGCTGTTGCTGTCAAGCAACACAAATTGGCTAGTTCTCAGGGGGATCAAGAGTTTTGTTCCGAAGTAGAAGTCCTGAGCTGTGCTCAGCACCGAAATGTTGTCATGTTGATTGGCTTCTGTATTGAGGACAAACGAAGATTGCTGGTCTATGAATATATTTGCAATGGGTCATTAGATTCTCATCTATACAGTAATATTTCTTTTTCTGACAACAGGCGACATCGCGAGCCTTTAGAATGGTCTGCACGGCAAAAGATCGCTGTGGGAGCAGCTCGAGGACTGCGATATCTTCATGAAGAATGCAGGGTGGGTTGCATTGTGCACCGTGACATGCGGCCAAACAACATCCTCATCACCCATGATTTTGAGCCACTGGTTGGGGATTTTGGCCTTGCAAGGTGGCAGCCTGATGGGGACACAGGCGTGGACACAAGAGTTATTGGAACATTTGGATATTTGGCTCCTGAATATGCTCAAAGTGGCCAAATCACAGAAAAGGCTGATGTTTATTCCTTTGGGGTGGTGTTGGTGGAACTTGTTACTGGGCGAAAAGCTGTGGACCTTAACCGGCCTAAGGGGCAGCAATGCCTCACAGAATGGGCACGCCCATTATTGGAAGAATATGCCATTGATGACCTGATTGACCCAAGACTTGACAACTTCTATTCGGAACAAGAGGTTTATTGCATGCTGCATGCTGCCTCTCTATGCATTCGGCGGGATCCTCAATCTAGGCCTCGCATGTCACAGGTTTTGCGTATGCTAGAAGGTGACATGGTAATGGACACAAATTACGCATCAACACCCGGGTATGATGTGGGGTGCCGGAATGGTCATGATGTGGGATGCCGGAGTGGTCGGATTTGGTCAGAGCATCAACAGCAGCACCAACCGCAGGAGAAGGAACGCTATAGTGGTCCACTACTAGATGAACCGATGGAAGGGTATAAGAAGCTCTCTCTTGAGAACGTACGGCCAGGTTTTTGGGAAAGGGACAAGGCAAGGAGGACTTCATCTGAACACCATTTGTAA

Protein Analysis

772

Amino Acids

85.92

Weight (kDa)

6.51

Isoelectric Point (pI)

48.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 296
AccB1I GGYRCC 2 cut(s) 1147, 2106
AccB7I CCANNNNNTGG 1 cut(s) 1661
AccII CGCG 1 cut(s) 1509
AciI CCGC 5 cut(s) 19, 1187, 1618, 2002, 2177
AclWI GGATC 7 cut(s) 295, 320, 541, 722, 1335, 2000, 2013
AcoI YGGCCR 5 cut(s) 66, 1279, 1619, 1765, 2258
AcsI RAATTY 6 cut(s) 87, 188, 523, 628, 896, 998
AcuI CTGAAG 3 cut(s) 290, 846, 1257
AcyI GRCGYC 2 cut(s) 48, 77
AfaI GTAC 1 cut(s) 2256
AfiI CCNNNNNNNGG 8 cut(s) 639, 674, 1157, 1661, 1858, 1897, 2179, 2225
AgsI TTSAA 5 cut(s) 131, 523, 529, 628, 1131
AhlI ACTAGT 1 cut(s) 659
AjnI CCWGG 4 cut(s) 133, 730, 1281, 2260
AjuI GAANNNNNNNTTGG 2 cut(s) 1230, 1262
AleI CACNNNNGTG 1 cut(s) 1706
Alw21I GWGCWC 3 cut(s) 267, 1369, 1608
Alw26I GTCTC 2 cut(s) 246, 762
Alw44I GTGCAC 1 cut(s) 1604
AlwI GGATC 7 cut(s) 295, 320, 541, 722, 1335, 2000, 2013
AlwNI CAGNNNCTG 2 cut(s) 695, 836
Ama87I CYCGRG 2 cut(s) 1557, 2090
ApaLI GTGCAC 1 cut(s) 1604
ApeKI GCWGC 8 cut(s) 310, 469, 1553, 1687, 1864, 1975, 1982, 2167
ApoI RAATTY 6 cut(s) 87, 188, 523, 628, 896, 998
ArsI GACNNNNNNTTYG 2 cut(s) 1338, 1370
AspS9I GGNCC 4 cut(s) 111, 641, 1843, 2201
AsuC2I CCSGG 2 cut(s) 2091, 2092
AsuHPI GGTGA 5 cut(s) 34, 360, 732, 1630, 2066
AsuII TTCGAA 1 cut(s) 1004
AvaI CYCGRG 2 cut(s) 1557, 2090
AvaII GGWCC 2 cut(s) 1843, 2201
AxyI CCTNAGG 1 cut(s) 1857
BaeGI GKGCMC 2 cut(s) 1608, 1888
BalI TGGCCA 2 cut(s) 1281, 1767
BamHI GGATCC 1 cut(s) 2005
BanI GGYRCC 2 cut(s) 1147, 2106
BanII GRGCYC 1 cut(s) 267
Bbv12I GWGCWC 3 cut(s) 267, 1369, 1608
BbvI GCAGC 8 cut(s) 297, 456, 1565, 1699, 1876, 1962, 1969, 2179
BccI CCATC 4 cut(s) 925, 1271, 1688, 2215
BceAI ACGGC 3 cut(s) 53, 1547, 2273
BcgI CGANNNNNNTGC 2 cut(s) 1001, 1035
BciT130I CCWGG 4 cut(s) 135, 732, 1283, 2262
BcnI CCSGG 2 cut(s) 2091, 2092
BcoDI GTCTC 2 cut(s) 246, 762
BcuI ACTAGT 1 cut(s) 659
BfaI CTAG 8 cut(s) 660, 674, 992, 1095, 1314, 2016, 2048, 2210
BfmI CTRYAG 3 cut(s) 387, 470, 2194
BfuAI ACCTGC 1 cut(s) 296
BglI GCCNNNNNGGC 3 cut(s) 74, 698, 1861
BisI GCNGC 9 cut(s) 311, 470, 1554, 1619, 1688, 1865, 1976, 1983, 2168
BlpI GCTNAGC 1 cut(s) 1368
BlsI GCNGC 9 cut(s) 312, 471, 1555, 1620, 1689, 1866, 1977, 1984, 2169
Bme1390I CCNGG 6 cut(s) 135, 732, 1283, 2091, 2092, 2262
Bme18I GGWCC 2 cut(s) 1843, 2201
BmeT110I CYCGRG 2 cut(s) 1557, 2090
BmgT120I GGNCC 4 cut(s) 111, 641, 1843, 2201
BmiI GGNNCC 7 cut(s) 40, 549, 878, 1149, 1746, 2007, 2108
BmrFI CCNGG 6 cut(s) 135, 732, 1283, 2091, 2092, 2262
BmrI ACTGGG 1 cut(s) 1836
BmsI GCATC 6 cut(s) 442, 980, 1009, 2090, 2120, 2167
BmuI ACTGGG 1 cut(s) 1836
BplI GAGNNNNNCTC 4 cut(s) 23, 55, 1351, 1383
BpmI CTGGAG 4 cut(s) 156, 1092, 1214, 1227
Bpu10I CCTNAGC 1 cut(s) 1358
Bpu1102I GCTNAGC 1 cut(s) 1368
Bpu14I TTCGAA 1 cut(s) 1004
BpuEI CTTGAG 4 cut(s) 251, 390, 1203, 2267
BpuMI CCSGG 2 cut(s) 2091, 2092
BsaBI GATNNNNATC 2 cut(s) 600, 1464
BsaHI GRCGYC 2 cut(s) 48, 77
BsaJI CCNNGG 4 cut(s) 81, 884, 1169, 2090
BsaWI WCCGGW 1 cut(s) 234
Bsc4I CCNNNNNNNGG 8 cut(s) 639, 674, 1157, 1661, 1858, 1897, 2179, 2225
Bse118I RCCGGY 1 cut(s) 1851
Bse1I ACTGG 6 cut(s) 225, 411, 1109, 1210, 1662, 1831
Bse21I CCTNAGG 1 cut(s) 1857
Bse3DI GCAATG 2 cut(s) 1453, 1874
Bse8I GATNNNNATC 2 cut(s) 600, 1464
BseBI CCWGG 4 cut(s) 135, 732, 1283, 2262
BseDI CCNNGG 4 cut(s) 81, 884, 1169, 2090
BseGI GGATG 6 cut(s) 601, 917, 971, 1024, 1629, 2135
BseJI GATNNNNATC 2 cut(s) 600, 1464
BseLI CCNNNNNNNGG 8 cut(s) 639, 674, 1157, 1661, 1858, 1897, 2179, 2225
BseMI GCAATG 2 cut(s) 1453, 1874
BseMII CTCAG 8 cut(s) 273, 308, 590, 687, 1064, 1334, 1349, 1382
BseNI ACTGG 6 cut(s) 225, 411, 1109, 1210, 1662, 1831
BseRI GAGGAG 2 cut(s) 1095, 1107
BseSI GKGCMC 2 cut(s) 1608, 1888
BseXI GCAGC 8 cut(s) 297, 456, 1565, 1699, 1876, 1962, 1969, 2179
BseYI CCCAGC 1 cut(s) 501
BsgI GTGCAG 1 cut(s) 1512
Bsh1236I CGCG 1 cut(s) 1509
BshNI GGYRCC 2 cut(s) 1147, 2106
BsiHKAI GWGCWC 3 cut(s) 267, 1369, 1608
BsiHKCI CYCGRG 2 cut(s) 1557, 2090
BsiSI CCGG 5 cut(s) 235, 1852, 2091, 2110, 2134
BsiWI CGTACG 1 cut(s) 2254
BslFI GGGAC 7 cut(s) 149, 230, 604, 691, 1097, 1712, 2291
BslI CCNNNNNNNGG 8 cut(s) 639, 674, 1157, 1661, 1858, 1897, 2179, 2225
BsmAI GTCTC 2 cut(s) 246, 762
BsmFI GGGAC 7 cut(s) 149, 230, 604, 691, 1097, 1712, 2291
BsmI GAATGC 3 cut(s) 1038, 1589, 1995
BsoBI CYCGRG 2 cut(s) 1557, 2090
Bsp119I TTCGAA 1 cut(s) 1004
Bsp1286I GDGCHC 4 cut(s) 267, 1369, 1608, 1888
Bsp143I GATC 7 cut(s) 300, 325, 533, 727, 1327, 1539, 2005
Bsp1720I GCTNAGC 1 cut(s) 1368
Bsp19I CCATGG 1 cut(s) 884
Bsp68I TCGCGA 1 cut(s) 1509
BspACI CCGC 5 cut(s) 19, 1187, 1618, 2002, 2177
BspCNI CTCAG 8 cut(s) 272, 307, 589, 688, 1065, 1333, 1350, 1381
BspFNI CGCG 1 cut(s) 1509
BspHI TCATGA 2 cut(s) 1576, 2119
BspLI GGNNCC 7 cut(s) 40, 549, 878, 1149, 1746, 2007, 2108
BspMAI CTGCAG 1 cut(s) 474
BspMI ACCTGC 1 cut(s) 296
BspPI GGATC 7 cut(s) 295, 320, 541, 722, 1335, 2000, 2013
BspQI GCTCTTC 1 cut(s) 459
BspT104I TTCGAA 1 cut(s) 1004
BspT107I GGYRCC 2 cut(s) 1147, 2106
BsrDI GCAATG 2 cut(s) 1453, 1874
BsrFI RCCGGY 1 cut(s) 1851
BsrI ACTGG 6 cut(s) 225, 411, 1109, 1210, 1662, 1831
BssAI RCCGGY 1 cut(s) 1851
BssECI CCNNGG 4 cut(s) 81, 884, 1169, 2090
BssMI GATC 7 cut(s) 300, 325, 533, 727, 1327, 1539, 2005
BssNI GRCGYC 2 cut(s) 48, 77
BssT1I CCWWGG 3 cut(s) 81, 884, 1169
Bst2UI CCWGG 4 cut(s) 135, 732, 1283, 2262
Bst4CI ACNGT 5 cut(s) 221, 824, 842, 1475, 1610
Bst6I CTCTTC 1 cut(s) 459
BstACI GRCGYC 2 cut(s) 48, 77
BstBI TTCGAA 1 cut(s) 1004
BstC8I GCNNGC 5 cut(s) 1200, 1511, 1888, 1973, 1980
BstDSI CCRYGG 1 cut(s) 884
BstEII GGTNACC 1 cut(s) 230
BstF5I GGATG 6 cut(s) 601, 917, 971, 1024, 1629, 2135
BstFNI CGCG 1 cut(s) 1509
BstKTI GATC 7 cut(s) 303, 328, 536, 730, 1330, 1542, 2008
BstMAI GTCTC 2 cut(s) 246, 762
BstMBI GATC 7 cut(s) 300, 325, 533, 727, 1327, 1539, 2005
BstNI CCWGG 4 cut(s) 135, 732, 1283, 2262
BstNSI RCATGY 4 cut(s) 1618, 1975, 1982, 2029
BstPI GGTNACC 1 cut(s) 230
BstSCI CCNGG 6 cut(s) 133, 730, 1281, 2089, 2090, 2260
BstSFI CTRYAG 3 cut(s) 387, 470, 2194
BstSLI GKGCMC 2 cut(s) 1608, 1888
BstUI CGCG 1 cut(s) 1509
BstV1I GCAGC 8 cut(s) 297, 456, 1565, 1699, 1876, 1962, 1969, 2179
BstX2I RGATCY 3 cut(s) 533, 727, 2005
BstXI CCANNNNNNTGG 2 cut(s) 1231, 2268
BstYI RGATCY 3 cut(s) 533, 727, 2005
Bsu36I CCTNAGG 1 cut(s) 1857
BtgI CCRYGG 1 cut(s) 884
BtgZI GCGATG 1 cut(s) 1490
BtsCI GGATG 6 cut(s) 601, 917, 971, 1024, 1629, 2135
BtsI GCAGTG 3 cut(s) 149, 454, 479
BtsIMutI CAGTG 9 cut(s) 149, 232, 454, 479, 693, 834, 847, 1069, 1655
BtuMI TCGCGA 1 cut(s) 1509
BveI ACCTGC 1 cut(s) 296
Cac8I GCNNGC 5 cut(s) 1200, 1511, 1888, 1973, 1980
CaiI CAGNNNCTG 2 cut(s) 695, 836
CciI TCATGA 2 cut(s) 1576, 2119
Cfr10I RCCGGY 1 cut(s) 1851
Cfr13I GGNCC 4 cut(s) 111, 641, 1843, 2201
Cfr9I CCCGGG 1 cut(s) 2090
CseI GACGC 2 cut(s) 56, 66
Csp6I GTAC 1 cut(s) 2255
CviQI GTAC 1 cut(s) 2255
DpnI GATC 7 cut(s) 302, 327, 535, 729, 1329, 1541, 2007
DpnII GATC 7 cut(s) 300, 325, 533, 727, 1327, 1539, 2005
EaeI YGGCCR 5 cut(s) 66, 1279, 1619, 1765, 2258
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 3 cut(s) 81, 884, 1169
Eco147I AGGCCT 1 cut(s) 2020
Eco24I GRGCYC 1 cut(s) 267
Eco32I GATATC 1 cut(s) 1571
Eco47I GGWCC 2 cut(s) 1843, 2201
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 3 cut(s) 290, 846, 1257
Eco81I CCTNAGG 1 cut(s) 1857
Eco88I CYCGRG 2 cut(s) 1557, 2090
Eco91I GGTNACC 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 265
EcoO109I RGGNCCY 1 cut(s) 641
EcoO65I GGTNACC 1 cut(s) 230
EcoRI GAATTC 1 cut(s) 628
EcoRII CCWGG 4 cut(s) 133, 730, 1281, 2260
EcoRV GATATC 1 cut(s) 1571
EcoT14I CCWWGG 3 cut(s) 81, 884, 1169
EcoT22I ATGCAT 1 cut(s) 1997
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 3 cut(s) 81, 884, 1169
FalI AAGNNNNNCTT 2 cut(s) 2278, 2310
FaqI GGGAC 7 cut(s) 149, 230, 604, 691, 1097, 1712, 2291
FauI CCCGC 2 cut(s) 12, 1995
Fnu4HI GCNGC 9 cut(s) 311, 470, 1554, 1619, 1688, 1865, 1976, 1983, 2168
FokI GGATG 6 cut(s) 588, 904, 958, 1031, 1616, 2142
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 9 cut(s) 311, 470, 1554, 1619, 1688, 1865, 1976, 1983, 2168
FspBI CTAG 8 cut(s) 660, 674, 992, 1095, 1314, 2016, 2048, 2210
GluI GCNGC 9 cut(s) 311, 470, 1554, 1619, 1688, 1865, 1976, 1983, 2168
GsaI CCCAGC 1 cut(s) 505
GsuI CTGGAG 4 cut(s) 156, 1092, 1214, 1227
HapII CCGG 5 cut(s) 235, 1852, 2091, 2110, 2134
HgaI GACGC 2 cut(s) 56, 66
Hin1I GRCGYC 2 cut(s) 48, 77
HincII GTYRAC 1 cut(s) 1139
HindII GTYRAC 1 cut(s) 1139
HindIII AAGCTT 1 cut(s) 984
HinfI GANTC 7 cut(s) 4, 118, 620, 652, 679, 785, 1460
HpaII CCGG 5 cut(s) 235, 1852, 2091, 2110, 2134
HphI GGTGA 5 cut(s) 34, 360, 732, 1630, 2066
Hpy166II GTNNAC 6 cut(s) 944, 1139, 1606, 1711, 1843, 2204
Hpy8I GTNNAC 6 cut(s) 944, 1139, 1606, 1711, 1843, 2204
HpyCH4III ACNGT 5 cut(s) 221, 824, 842, 1475, 1610
HpyCH4IV ACGT 1 cut(s) 2253
HpySE526I ACGT 1 cut(s) 2253
Hsp92I GRCGYC 2 cut(s) 48, 77
Kzo9I GATC 7 cut(s) 300, 325, 533, 727, 1327, 1539, 2005
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 6 cut(s) 44, 1108, 1189, 1195, 1550, 1750
Lsp1109I GCAGC 8 cut(s) 297, 456, 1565, 1699, 1876, 1962, 1969, 2179
LweI GCATC 6 cut(s) 442, 980, 1009, 2090, 2120, 2167
MaeI CTAG 8 cut(s) 660, 674, 992, 1095, 1314, 2016, 2048, 2210
MaeII ACGT 1 cut(s) 2253
MaeIII GTNAC 6 cut(s) 230, 649, 1610, 1822, 2028, 2054
MalI GATC 7 cut(s) 302, 327, 535, 729, 1329, 1541, 2007
MboI GATC 7 cut(s) 300, 325, 533, 727, 1327, 1539, 2005
MflI RGATCY 3 cut(s) 533, 727, 2005
MhlI GDGCHC 4 cut(s) 267, 1369, 1608, 1888
MlsI TGGCCA 2 cut(s) 1281, 1767
MluNI TGGCCA 2 cut(s) 1281, 1767
MlyI GAGTC 5 cut(s) 13, 112, 614, 646, 673
MmeI TCCRAC 3 cut(s) 680, 904, 2123
Mox20I TGGCCA 2 cut(s) 1281, 1767
Mph1103I ATGCAT 1 cut(s) 1997
MscI TGGCCA 2 cut(s) 1281, 1767
MseI TTAA 4 cut(s) 72, 438, 903, 1848
MslI CAYNNNNRTG 3 cut(s) 1377, 1706, 2063
Msp20I TGGCCA 2 cut(s) 1281, 1767
MspA1I CMGCKG 1 cut(s) 310
MspI CCGG 5 cut(s) 235, 1852, 2091, 2110, 2134
MspR9I CCNGG 6 cut(s) 135, 732, 1283, 2091, 2092, 2262
Mva1269I GAATGC 3 cut(s) 1038, 1589, 1995
MvaI CCWGG 4 cut(s) 135, 732, 1283, 2262
MvnI CGCG 1 cut(s) 1509
NciI CCSGG 2 cut(s) 2091, 2092
NcoI CCATGG 1 cut(s) 884
NdeII GATC 7 cut(s) 300, 325, 533, 727, 1327, 1539, 2005
NlaIV GGNNCC 7 cut(s) 40, 549, 878, 1149, 1746, 2007, 2108
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 4 cut(s) 649, 1610, 2028, 2054
NruI TCGCGA 1 cut(s) 1509
NsiI ATGCAT 1 cut(s) 1997
NspI RCATGY 4 cut(s) 1618, 1975, 1982, 2029
NspV TTCGAA 1 cut(s) 1004
OliI CACNNNNGTG 1 cut(s) 1706
PaeI GCATGC 2 cut(s) 1975, 1982
PaeR7I CTCGAG 1 cut(s) 1557
PagI TCATGA 2 cut(s) 1576, 2119
PceI AGGCCT 1 cut(s) 2020
PciSI GCTCTTC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 1 cut(s) 1564
PctI GAATGC 3 cut(s) 1038, 1589, 1995
PfeI GAWTC 2 cut(s) 785, 1460
Pfl23II CGTACG 1 cut(s) 2254
PflMI CCANNNNNTGG 1 cut(s) 1661
PfoI TCCNGGA 1 cut(s) 730
PkrI GCNGC 9 cut(s) 312, 471, 1555, 1620, 1689, 1866, 1977, 1984, 2169
PleI GAGTC 5 cut(s) 12, 112, 614, 646, 673
PpsI GAGTC 5 cut(s) 12, 112, 614, 646, 673
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 4 cut(s) 133, 730, 1281, 2260
PspEI GGTNACC 1 cut(s) 230
PspFI CCCAGC 1 cut(s) 501
PspGI CCWGG 4 cut(s) 133, 730, 1281, 2260
PspLI CGTACG 1 cut(s) 2254
PspN4I GGNNCC 7 cut(s) 40, 549, 878, 1149, 1746, 2007, 2108
PspPI GGNCC 4 cut(s) 111, 641, 1843, 2201
PspXI VCTCGAGB 1 cut(s) 1557
PstI CTGCAG 1 cut(s) 474
PstNI CAGNNNCTG 2 cut(s) 695, 836
PsuI RGATCY 3 cut(s) 533, 727, 2005
PvuII CAGCTG 1 cut(s) 310
RruI TCGCGA 1 cut(s) 1509
RsaI GTAC 1 cut(s) 2256
RsaNI GTAC 1 cut(s) 2255
RseI CAYNNNNRTG 3 cut(s) 1377, 1706, 2063
SacI GAGCTC 1 cut(s) 267
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 4 cut(s) 72, 438, 903, 1848
SatI GCNGC 9 cut(s) 311, 470, 1554, 1619, 1688, 1865, 1976, 1983, 2168
Sau3AI GATC 7 cut(s) 300, 325, 533, 727, 1327, 1539, 2005
Sau96I GGNCC 4 cut(s) 111, 641, 1843, 2201
SchI GAGTC 5 cut(s) 13, 112, 614, 646, 673
ScrFI CCNGG 6 cut(s) 135, 732, 1283, 2091, 2092, 2262
SduI GDGCHC 4 cut(s) 267, 1369, 1608, 1888
SfaNI GCATC 6 cut(s) 442, 980, 1009, 2090, 2120, 2167
SfcI CTRYAG 3 cut(s) 387, 470, 2194
Sfr274I CTCGAG 1 cut(s) 1557
SfuI TTCGAA 1 cut(s) 1004
SinI GGWCC 2 cut(s) 1843, 2201
SlaI CTCGAG 1 cut(s) 1557
SmaI CCCGGG 1 cut(s) 2092
SmiMI CAYNNNNRTG 3 cut(s) 1377, 1706, 2063
SmlI CTYRAG 5 cut(s) 266, 405, 1218, 1557, 2246
SmoI CTYRAG 5 cut(s) 266, 405, 1218, 1557, 2246
SpeI ACTAGT 1 cut(s) 659
SphI GCATGC 2 cut(s) 1975, 1982
SseBI AGGCCT 1 cut(s) 2020
SsiI CCGC 5 cut(s) 19, 1187, 1618, 2002, 2177
SspI AATATT 1 cut(s) 1480
SspMI CTAG 8 cut(s) 660, 674, 992, 1095, 1314, 2016, 2048, 2210
SstI GAGCTC 1 cut(s) 267
StuI AGGCCT 1 cut(s) 2020
StyD4I CCNGG 6 cut(s) 133, 730, 1281, 2089, 2090, 2260
StyI CCWWGG 3 cut(s) 81, 884, 1169
TaaI ACNGT 5 cut(s) 221, 824, 842, 1475, 1610
TaiI ACGT 1 cut(s) 2256
TaqI TCGA 5 cut(s) 7, 637, 1004, 1011, 1558
TauI GCSGC 1 cut(s) 1621
TfiI GAWTC 2 cut(s) 785, 1460
Tru1I TTAA 4 cut(s) 72, 438, 903, 1848
Tru9I TTAA 4 cut(s) 72, 438, 903, 1848
TscAI CASTG 9 cut(s) 156, 232, 461, 479, 700, 841, 847, 1069, 1662
TseFI GTSAC 4 cut(s) 649, 1610, 2028, 2054
TseI GCWGC 8 cut(s) 310, 469, 1553, 1687, 1864, 1975, 1982, 2167
Tsp45I GTSAC 4 cut(s) 649, 1610, 2028, 2054
TspMI CCCGGG 1 cut(s) 2090
TspRI CASTG 9 cut(s) 156, 232, 461, 479, 700, 841, 847, 1069, 1662
Van91I CCANNNNNTGG 1 cut(s) 1661
VneI GTGCAC 1 cut(s) 1604
VpaK11BI GGWCC 2 cut(s) 1843, 2201
XapI RAATTY 6 cut(s) 87, 188, 523, 628, 896, 998
XbaI TCTAGA 1 cut(s) 1094
XceI RCATGY 4 cut(s) 1618, 1975, 1982, 2029
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XhoI CTCGAG 1 cut(s) 1557
XmaI CCCGGG 1 cut(s) 2090
XspI CTAG 8 cut(s) 660, 674, 992, 1095, 1314, 2016, 2048, 2210
Zsp2I ATGCAT 1 cut(s) 1997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.