MD09G1225000.v1.1

ABC transporter C family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002799
Physical Location & Seq
Reverse (-)
27690685 .. 27709554
18870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1225000.v1.1.491

Sequence Viewer

Length: 4263 bp
ATGATAGTTGGGCTCGGAATAACACAACGGACTGGCAGAAGAAGTTGGAGGATGAACTTCATGGAAAAGCTTTTCTTGCTCTTTCTTCCTGCCATTGGAGTATGCATATCATTTTTAGACGGTGCATTACTGTTGAAGAAAGCGCTTGGCATCTTTGTCGCACATCATGAATGGTTTTTCAGATGTTCTCAATTTGCATCATGGACACTTATCATACTGTTCTCAAAGTATTTCAACGGTTGTCAAATTTTCTGCAACCGGATCTTGTGTTTCTGGTGGATTATAAAACTTCTTCTGGGAATACTTCATTTGCTCACAGCATATCCTCCATTCAAGGTCTTATTATGTGTTACTGTTACATTGGACACGATCTTTGGTATATCGATTAACATTATCAGAATAAAGCGGGCATCTTACAAGAGAAGTACAGTTGAAGATTCACTACTTTCAGCTGACACAGATGTTGAGGAAGGCAGCCTTAATGATTCTGGAAACACTCAGGGCTATTTCGATCTTTTGACATTTAGATCTATCACTTCTGTGATGAATCATGGGGTCACAAAGCAGATTGATTTTGAGGATTTGCTTCACCTTCCTTCTGATATGAATCCCTGTTCCTGTCATGATACATTGTTTGGCTGTTGGCAATCCCAACAGAGCAGTTCCCCTGATCCATCACTGTTTAGGGCAATATGTTGTGCATATGGATGGCCATACATTCGTTTGGGCTTGTTGAAGGTACTTAATGACTCTGTTGGTTTTGCGGGACCACTCCTTCTCAATAAACTGATTCGCTTCCTTCAACAAGGTTCGAAGAATATTGATGGCTATGTTCTTGCAATATGCTTGGGCCTCGTATCTATTTTGAAATCAGTTTTGGATACGCAATATACTTTTCATCTTTCAAGACTGAAGTTAAAGTTACGATCTAGTATCATTACTATTATCTATCAGAAGTGCTTAAGTATCAATCTAGCAGAACGATCACAATTTACTGAGGGAGAAATCCAGACATTCATGGCTGTAGATTCTGATCGTACTGTCAACTTGTGTAACAGTTTCCATGATATGTGGAGCTTGCCATTACAAATTGGGGTGGCTTTGTTCCTTCTGTATACACAAGTCAAGTTTGCATTTGTTTCGGGTGTTGCAATTACCATCGCACTGATACCAGTGAATAAATGGATATCTACATTGATTGCAAGTGCTACAATGAAAATGATGCAGCAGAAGGATGAGAGGATAAGGAGGACAGGAGAACTTTTGACATATATCCGCACTTTGAAGATGTATGGGTGGGAGCTTCTGTTTTCTAGCTGGTTGATGGAGACGAGATCATTAGAAGTTATGCACTTAACTACACGGAAGTACTTGGATGCCTGGTGTGTATTTTTTTGGGCTACAACACCAACTCTTTTTTCTCTGTTCACATTTGGGCTTTTCACATTGATGGGACATCAACTTGATGCTGCAACGGTCTTCACCTGTCTTGCATTGTTTAATAATTTGATATCCCCTCTAAATTCATTTCCATGGGTCATTAATGGATTAATTGATGCCATCATATCTCTCAAGCGGTTGACCAGATTTCTGTCTTGCTCTGAGCACAAATCCAAACTAGAAAAGACAGCTGGTTCTGCTTCATCATATATTTCAAATGACCAATCTGAGTTTACTCATGAAGATAAGGCTGTTGTGTTCCATGATTCCTGCTGTTCATGGTCAAGCAGCGATGAAGAGCAATTGAATTTGGTTTTGAATCATGTGACTCTTGCTATTCCGAAGGGTTCCTTTGTTGCAGTAATTGGAGAGGTCGGTTCTGGTAAATCATCTTTGTTGAGTTCAATTTTGGGGGAGATGCAACTTGTCCATGGATCAGTGTATTCCTGTGGTTCTATAGCATATGTACCACAGGTCCCCTGGATTCTATCTGGAACAATACGTGATAACATACTGTTTGGAAAGCATTACGACCCCAGAAGATACTCTGACACTTTAGAGGCAAGTGCGCTAAACCTCGATATTTCATTAATGGTTGGAGGTGACATGGCTTACATTGGAGAAAAGGGAATCAATTTGTCAGGTGGACAGAGAGCTAGAATTGCTTTGGCAAGGGCTATTTATAATGGCTCTGATATGTTTATTCTTGATGACGTCCTTAGTGCAGTTGATGCACAAGTTGCTCGCTGTATTTTATATAAAGCCATTTTGGGTCCACTTATGAACCAGCAGACTCGTGTACTTTGTACCCATAATGTTCAGGCAATATCTTCTGCTGATAGAATAGTTGTCATGGATAAGGGACATGTGAAATGGGTTGGAAGCTCAGCTGATTTGCCAGTTTCTTTGTGTTCAGCATTCTCTCCACTGAATGAATTTGATACTTGTTTACAAAATGAAAGACAAGAGTCCAGTGTCGTAGATACTCTTATGGAAAGCCAACAAACTCTCATCCTAGAAAAAGAAACTGTGCCTGCTTCAGATGGAACACAAGAGATTATTGAAGTTGAGGCGCGAAAAGAGGGCAGAGTAGAACTTACCATCTACAAGAAATATGCCACATTTTCTGGTTGGTTGATTTCAGTTTTAATATGCCTCTCAGCAGTCTTAATGCAAGCTTCTCGTAATGGGAATGATCTGTGGCTCTCAAATTGGGTTGATGCAACAGGAAGAGGTCAGAAAGAATATTCCACATCCTTTTATCTGGTTATGCTTTGCATCTTTTGCATTGTAAATTCAATTCTCACACTAGTGAGGGCCTTCTCATTCGCATTTGGTGGCTTACGAGCTGCTGTTAAGGTGCACGATACACTGCTGAAAAGGATTATCAATGCCCCTGTGCAATTTTTCGATCAGACACCAGGTGGAAGAATGCTAAACAGGTTTTCTTCAGATCTATATACAATTGATGATTCTCTTCCCTTCATTCTTAACATTCTCCTAGCCAATTTTGTTGGCCTGCTGGGCATTGCAATAGTTTTGTCATATGTTCAGGTCTTCTTCTTGCTTTTGTTATTCCCATTCTGGTACATCTACAACCAACTTCAGTGCTTCTACAGATCAACTTCACGGGAATTGCGAAGGCTAGACAGTGTTTCTCGATCTCCCATCTATACATCCTTCTCAGAGACACTTGATGGATCATCAACTATACGGGCATTCAAGTCTGAGGACCTCTTTTTCGGCAGATTCACTGACCAGGTCAAATTGTATCAACAAACTTCATACACAGAGTTAACAGCAAGTTTGTGGCTTTCCCTTCGTCTTCAGTTGTTAGCTGCATTTATCATCTCGTTTATTGCTATCATGGCTGTTATTGGATCTCGTGGAAGTTTACCAATCAATTTCAGTACTCCAGGGTTGGTAGGATTGGCTCTGTCTTATGCAGCTCCAGTTGTCTCATTACTTGGAAGCTTTTTGACAAGTTTCACTGAAACAGAGAAGGAAATGGTTTCTGTTGAAAGAGCTCTTGAGTATATGGACGTTCCGCAAGAAGAAATGGATGGATTCCAATCCTTACATCCCAGTTGGCCTTATCAAGGACAGGTTGAATTCCACAATGTCACCTTGAGATATAAGCCGTCCTTGCCAGCTGCACTTCGTGATGTTAGTTTTACTATTGAAGGAGGGATGGAGGTCGGTATCATTGGAAGGACAGGAGCTGGAAAATCTAGTGTATTGAATGCCCTTTTCCGCCTCACACCAATATGTACAGGATGCATTCTTGTGGATGCCATAAACATAGCTAATGCTCCTATCAGGGATCTTCGTGCACATTTCTCTGTTGTTCCTCAGACTCCATTTTTATTTGAAGGATCATTGAGGGACAACCTTGATCCATTCCGGCTGTGTGATGATATAAACATTTGGAAGGCTTTGGAGAGATGCCATGTTAAAGAGGAAGTAGAAGCAGCAGGGGGGCTAGATATTCACCTAAAGGAATCTAGGATGTCGTTTTCTGTTGGGCAACGGCAGCTTCTTTGCCTTGCACGTGCACTTCTCAGGTCTTCAAAGGTCCTGTGTTTGGATGAATGCACCGCTAATGTTGACACTCAAACAGCATCCATATTACAAAAAACCGTATCCAGTGAATGCAGAGGAATGACAGTTATCACAATTGCTCACCGTATTTCCACTGTCTTGAATATGGACAAGGTCCTGGTCCTTGACCATGGTATGCTGGTTGAACAAGGGAATCCGCAGGCTCTCCTAGAAAATGAGTTCTCCAGATTTTCAAGTTTTGCTAAAGCTTCGACAATGTGA

Protein Analysis

1421

Amino Acids

158.75

Weight (kDa)

6.71

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 243 - 507 2.5e-18 ABC transporter transmembrane region
ABC_tran PF00005 586 - 719 2.7e-16 ABC transporter
ABC_membrane PF00664 863 - 1108 7.2e-38 ABC transporter transmembrane region
ABC_tran PF00005 1200 - 1347 3.7e-24 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 284, 2124
AatII GACGTC 1 cut(s) 2157
AccI GTMKAC 1 cut(s) 1115
AccII CGCG 1 cut(s) 2515
AciI CCGC 8 cut(s) 406, 764, 1276, 1576, 3488, 3694, 4038, 4199
AclWI GGATC 8 cut(s) 269, 665, 1882, 3148, 3328, 3771, 3823, 3830
AcoI YGGCCR 1 cut(s) 710
AcsI RAATTY 6 cut(s) 246, 1522, 1747, 2375, 2734, 3551
AcuI CTGAAG 5 cut(s) 932, 2463, 2874, 3029, 3251
AcvI CACGTG 1 cut(s) 3992
AcyI GRCGYC 1 cut(s) 2154
AdeI CACNNNGTG 2 cut(s) 2864, 3602
AfeI AGCGCT 1 cut(s) 144
AfiI CCNNNNNNNGG 3 cut(s) 95, 3539, 4024
AflII CTTAAG 1 cut(s) 961
AflIII ACRYGT 1 cut(s) 2305
AhlI ACTAGT 1 cut(s) 2749
AjnI CCWGG 6 cut(s) 1379, 1919, 2860, 3198, 3355, 4158
AjuI GAANNNNNNNTTGG 4 cut(s) 860, 892, 1402, 1434
AleI CACNNNNGTG 2 cut(s) 539, 2750
Alw21I GWGCWC 5 cut(s) 1608, 2805, 3469, 3775, 3997
Alw26I GTCTC 3 cut(s) 1322, 3122, 3403
Alw44I GTGCAC 3 cut(s) 2801, 3771, 3993
AlwI GGATC 8 cut(s) 269, 665, 1882, 3148, 3328, 3771, 3823, 3830
AlwNI CAGNNNCTG 1 cut(s) 3662
Aor51HI AGCGCT 1 cut(s) 144
AoxI GGCC 5 cut(s) 710, 850, 2757, 2956, 3530
ApaLI GTGCAC 3 cut(s) 2801, 3771, 3993
ApoI RAATTY 6 cut(s) 246, 1522, 1747, 2375, 2734, 3551
ArsI GACNNNNNNTTYG 4 cut(s) 356, 388, 3164, 3196
AseI ATTAAT 3 cut(s) 1542, 1550, 2030
AspLEI GCGC 3 cut(s) 145, 2011, 2515
AspS9I GGNCC 9 cut(s) 767, 850, 1915, 2213, 2757, 3172, 4015, 4156, 4162
AsuHPI GGTGA 6 cut(s) 581, 1474, 2054, 3556, 3923, 4115
AsuII TTCGAA 1 cut(s) 812
AvaII GGWCC 7 cut(s) 767, 1915, 2213, 3172, 4015, 4156, 4162
BaeGI GKGCMC 3 cut(s) 2805, 3775, 3997
BalI TGGCCA 1 cut(s) 712
BanII GRGCYC 2 cut(s) 15, 3469
BarI GAAGNNNNNNTAC 2 cut(s) 426, 458
BauI CACGAG 2 cut(s) 2235, 3324
BbrPI CACGTG 1 cut(s) 3992
BbsI GAAGAC 4 cut(s) 1471, 2989, 3257, 3999
Bbv12I GWGCWC 5 cut(s) 1608, 2805, 3469, 3775, 3997
BceAI ACGGC 2 cut(s) 3565, 3986
BcgI CGANNNNNNTGC 6 cut(s) 139, 173, 1122, 1156, 2603, 2637
BciT130I CCWGG 6 cut(s) 1381, 1921, 2862, 3200, 3357, 4160
BciVI GTATCC 2 cut(s) 874, 4093
BcoDI GTCTC 3 cut(s) 1322, 3122, 3403
BcuI ACTAGT 1 cut(s) 2749
BfmI CTRYAG 3 cut(s) 1023, 1896, 3055
BfoI RGCGCY 1 cut(s) 146
BfrI CTTAAG 1 cut(s) 961
BfuI GTATCC 2 cut(s) 874, 4093
BglI GCCNNNNNGGC 1 cut(s) 2964
BglII AGATCT 2 cut(s) 527, 2893
BlpI GCTNAGC 1 cut(s) 2326
BmcAI AGTACT 2 cut(s) 1370, 3352
Bme1390I CCNGG 6 cut(s) 1381, 1921, 2862, 3200, 3357, 4160
Bme18I GGWCC 7 cut(s) 767, 1915, 2213, 3172, 4015, 4156, 4162
BmgT120I GGNCC 9 cut(s) 767, 850, 1915, 2213, 2757, 3172, 4015, 4156, 4162
BmiI GGNNCC 4 cut(s) 768, 1789, 1917, 2214
BmrFI CCNGG 6 cut(s) 1381, 1921, 2862, 3200, 3357, 4160
BmrI ACTGGG 1 cut(s) 3519
BmuI ACTGGG 1 cut(s) 3519
BoxI GACNNNNGTC 1 cut(s) 2407
BpiI GAAGAC 4 cut(s) 1471, 2989, 3257, 3999
BpmI CTGGAG 3 cut(s) 3339, 3375, 4210
Bpu1102I GCTNAGC 1 cut(s) 2326
Bpu14I TTCGAA 1 cut(s) 812
BpuEI CTTGAG 3 cut(s) 1556, 3491, 3589
Bsa29I ATCGAT 1 cut(s) 383
BsaAI YACGTR 2 cut(s) 1943, 3992
BsaBI GATNNNNATC 3 cut(s) 606, 1032, 3511
BsaHI GRCGYC 1 cut(s) 2154
BsaJI CCNNGG 5 cut(s) 1532, 1870, 1919, 3356, 4171
BsaWI WCCGGW 1 cut(s) 258
BsaXI ACNNNNNCTCC 2 cut(s) 1292, 1322
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 3539, 4024
Bse1I ACTGG 7 cut(s) 37, 1172, 2339, 2412, 3392, 3525, 4086
Bse3DI GCAATG 1 cut(s) 2967
Bse8I GATNNNNATC 3 cut(s) 606, 1032, 3511
BseBI CCWGG 6 cut(s) 1381, 1921, 2862, 3200, 3357, 4160
BseCI ATCGAT 1 cut(s) 383
BseDI CCNNGG 5 cut(s) 1532, 1870, 1919, 3356, 4171
BseJI GATNNNNATC 3 cut(s) 606, 1032, 3511
BseLI CCNNNNNNNGG 3 cut(s) 95, 3539, 4024
BseMI GCAATG 1 cut(s) 2967
BseNI ACTGG 7 cut(s) 37, 1172, 2339, 2412, 3392, 3525, 4086
BseSI GKGCMC 3 cut(s) 2805, 3775, 3997
BseYI CCCAGC 1 cut(s) 2962
BsgI GTGCAG 2 cut(s) 2184, 3579
Bsh1236I CGCG 1 cut(s) 2515
BshFI GGCC 5 cut(s) 712, 852, 2759, 2958, 3532
BshVI ATCGAT 1 cut(s) 383
BsiHKAI GWGCWC 5 cut(s) 1608, 2805, 3469, 3775, 3997
BsiSI CCGG 2 cut(s) 259, 3844
BslFI GGGAC 5 cut(s) 780, 1467, 1901, 2316, 3839
BslI CCNNNNNNNGG 3 cut(s) 95, 3539, 4024
BsmAI GTCTC 3 cut(s) 1322, 3122, 3403
BsmBI CGTCTC 1 cut(s) 1322
BsmFI GGGAC 5 cut(s) 780, 1467, 1901, 2316, 3839
BsmI GAATGC 7 cut(s) 2357, 2877, 3158, 3688, 3720, 4037, 4097
BsnI GGCC 5 cut(s) 712, 852, 2759, 2958, 3532
Bsp119I TTCGAA 1 cut(s) 812
Bsp1286I GDGCHC 6 cut(s) 15, 1608, 2805, 3469, 3775, 3997
Bsp1407I TGTACA 1 cut(s) 3710
Bsp1720I GCTNAGC 1 cut(s) 2326
Bsp19I CCATGG 3 cut(s) 1532, 1870, 4171
BspACI CCGC 8 cut(s) 406, 764, 1276, 1576, 3488, 3694, 4038, 4199
BspANI GGCC 5 cut(s) 712, 852, 2759, 2958, 3532
BspDI ATCGAT 1 cut(s) 383
BspFNI CGCG 1 cut(s) 2515
BspHI TCATGA 3 cut(s) 166, 622, 1678
BspLI GGNNCC 4 cut(s) 768, 1789, 1917, 2214
BspPI GGATC 8 cut(s) 269, 665, 1882, 3148, 3328, 3771, 3823, 3830
BspQI GCTCTTC 1 cut(s) 1731
BspT104I TTCGAA 1 cut(s) 812
BspTI CTTAAG 1 cut(s) 961
BsrDI GCAATG 1 cut(s) 2967
BsrGI TGTACA 1 cut(s) 3710
BsrI ACTGG 7 cut(s) 37, 1172, 2339, 2412, 3392, 3525, 4086
BssECI CCNNGG 5 cut(s) 1532, 1870, 1919, 3356, 4171
BssNAI GTATAC 1 cut(s) 1116
BssNI GRCGYC 1 cut(s) 2154
BssSI CACGAG 2 cut(s) 2235, 3324
BssT1I CCWWGG 3 cut(s) 1532, 1870, 4171
Bst1107I GTATAC 1 cut(s) 1116
Bst2BI CACGAG 2 cut(s) 2235, 3324
Bst2UI CCWGG 6 cut(s) 1381, 1921, 2862, 3200, 3357, 4160
Bst6I CTCTTC 3 cut(s) 1731, 2665, 2922
BstACI GRCGYC 1 cut(s) 2154
BstAFI CTTAAG 1 cut(s) 961
BstAPI GCANNNNNTGC 3 cut(s) 2171, 2180, 2724
BstAUI TGTACA 1 cut(s) 3710
BstBAI YACGTR 2 cut(s) 1943, 3992
BstBI TTCGAA 1 cut(s) 812
BstC8I GCNNGC 8 cut(s) 408, 1079, 2185, 2475, 2616, 2960, 3591, 4203
BstDSI CCRYGG 3 cut(s) 1532, 1870, 4171
BstENI CCTNNNNNAGG 1 cut(s) 3537
BstFNI CGCG 1 cut(s) 2515
BstH2I RGCGCY 1 cut(s) 146
BstHHI GCGC 3 cut(s) 145, 2011, 2515
BstMAI GTCTC 3 cut(s) 1322, 3122, 3403
BstNI CCWGG 6 cut(s) 1381, 1921, 2862, 3200, 3357, 4160
BstNSI RCATGY 1 cut(s) 2309
BstPAI GACNNNNGTC 1 cut(s) 2407
BstSCI CCNGG 6 cut(s) 1379, 1919, 2860, 3198, 3355, 4158
BstSFI CTRYAG 3 cut(s) 1023, 1896, 3055
BstSLI GKGCMC 3 cut(s) 2805, 3775, 3997
BstUI CGCG 1 cut(s) 2515
BstV2I GAAGAC 4 cut(s) 1471, 2989, 3257, 3999
BstX2I RGATCY 5 cut(s) 261, 527, 2893, 3320, 3763
BstYI RGATCY 5 cut(s) 261, 527, 2893, 3320, 3763
BstZ17I GTATAC 1 cut(s) 1116
Bsu15I ATCGAT 1 cut(s) 383
BsuI GTATCC 2 cut(s) 874, 4093
BsuRI GGCC 5 cut(s) 712, 852, 2759, 2958, 3532
BsuTUI ATCGAT 1 cut(s) 383
BtgI CCRYGG 3 cut(s) 1532, 1870, 4171
BtgZI GCGATG 2 cut(s) 1144, 1746
BtsI GCAGTG 1 cut(s) 2810
Cac8I GCNNGC 8 cut(s) 408, 1079, 2185, 2475, 2616, 2960, 3591, 4203
CaiI CAGNNNCTG 1 cut(s) 3662
CciI TCATGA 3 cut(s) 166, 622, 1678
CfoI GCGC 3 cut(s) 145, 2011, 2515
Cfr13I GGNCC 9 cut(s) 767, 850, 1915, 2213, 2757, 3172, 4015, 4156, 4162
ClaI ATCGAT 1 cut(s) 383
CsiI ACCWGGT 2 cut(s) 2860, 3198
DraIII CACNNNGTG 2 cut(s) 2864, 3602
EaeI YGGCCR 1 cut(s) 710
Eam1104I CTCTTC 3 cut(s) 1731, 2665, 2922
EarI CTCTTC 3 cut(s) 1731, 2665, 2922
EciI GGCGGA 1 cut(s) 3683
Ecl136II GAGCTC 1 cut(s) 3467
Eco130I CCWWGG 3 cut(s) 1532, 1870, 4171
Eco24I GRGCYC 2 cut(s) 15, 3469
Eco32I GATATC 2 cut(s) 1188, 1512
Eco47I GGWCC 7 cut(s) 767, 1915, 2213, 3172, 4015, 4156, 4162
Eco47III AGCGCT 1 cut(s) 144
Eco53kI GAGCTC 1 cut(s) 3467
Eco57I CTGAAG 5 cut(s) 932, 2463, 2874, 3029, 3251
Eco72I CACGTG 1 cut(s) 3992
EcoICRI GAGCTC 1 cut(s) 3467
EcoNI CCTNNNNNAGG 1 cut(s) 3537
EcoO109I RGGNCCY 5 cut(s) 1915, 2757, 3172, 4015, 4156
EcoRI GAATTC 1 cut(s) 3551
EcoRII CCWGG 6 cut(s) 1379, 1919, 2860, 3198, 3355, 4158
EcoRV GATATC 2 cut(s) 1188, 1512
EcoT14I CCWWGG 3 cut(s) 1532, 1870, 4171
EcoT22I ATGCAT 2 cut(s) 107, 3722
EcoT38I GRGCYC 2 cut(s) 15, 3469
ErhI CCWWGG 3 cut(s) 1532, 1870, 4171
Esp3I CGTCTC 1 cut(s) 1322
FalI AAGNNNNNCTT 8 cut(s) 59, 91, 462, 494, 1775, 1807, 3569, 3601
FaqI GGGAC 5 cut(s) 780, 1467, 1901, 2316, 3839
FauI CCCGC 2 cut(s) 399, 757
FauNDI CATATG 3 cut(s) 703, 1903, 2986
FblI GTMKAC 1 cut(s) 1115
FriOI GRGCYC 2 cut(s) 15, 3469
GlaI GCGC 3 cut(s) 144, 2010, 2514
GsaI CCCAGC 1 cut(s) 2966
GsuI CTGGAG 3 cut(s) 3339, 3375, 4210
HaeII RGCGCY 1 cut(s) 146
HaeIII GGCC 5 cut(s) 712, 852, 2759, 2958, 3532
HapII CCGG 2 cut(s) 259, 3844
HhaI GCGC 3 cut(s) 145, 2011, 2515
Hin1I GRCGYC 1 cut(s) 2154
Hin6I GCGC 3 cut(s) 143, 2009, 2513
HinP1I GCGC 3 cut(s) 143, 2009, 2513
HincII GTYRAC 4 cut(s) 1045, 1581, 3237, 4048
HindII GTYRAC 4 cut(s) 1045, 1581, 3237, 4048
HindIII AAGCTT 4 cut(s) 68, 2616, 3412, 4248
HpaI GTTAAC 1 cut(s) 3237
HpaII CCGG 2 cut(s) 259, 3844
HphI GGTGA 6 cut(s) 581, 1474, 2054, 3556, 3923, 4115
HpyCH4IV ACGT 4 cut(s) 1942, 2154, 3483, 3991
HpySE526I ACGT 4 cut(s) 1942, 2154, 3483, 3991
Hsp92I GRCGYC 1 cut(s) 2154
HspAI GCGC 3 cut(s) 143, 2009, 2513
KspAI GTTAAC 1 cut(s) 3237
LguI GCTCTTC 1 cut(s) 1731
LmnI GCTCC 5 cut(s) 1074, 1300, 3394, 3659, 3757
MabI ACCWGGT 2 cut(s) 2860, 3198
MaeII ACGT 4 cut(s) 1942, 2154, 3483, 3991
MaeIII GTNAC 8 cut(s) 349, 355, 556, 921, 1052, 1765, 2042, 3562
MfeI CAATTG 3 cut(s) 1742, 2904, 4116
MflI RGATCY 5 cut(s) 261, 527, 2893, 3320, 3763
MhlI GDGCHC 6 cut(s) 15, 1608, 2805, 3469, 3775, 3997
MlsI TGGCCA 1 cut(s) 712
MluNI TGGCCA 1 cut(s) 712
MlyI GAGTC 5 cut(s) 743, 1762, 2227, 2417, 3790
MmeI TCCRAC 3 cut(s) 26, 2017, 2299
Mox20I TGGCCA 1 cut(s) 712
Mph1103I ATGCAT 2 cut(s) 107, 3722
MscI TGGCCA 1 cut(s) 712
MslI CAYNNNNRTG 7 cut(s) 539, 706, 1448, 1531, 2750, 3706, 3725
Msp20I TGGCCA 1 cut(s) 712
MspA1I CMGCKG 4 cut(s) 452, 1631, 2330, 3593
MspCI CTTAAG 1 cut(s) 961
MspI CCGG 2 cut(s) 259, 3844
MspR9I CCNGG 6 cut(s) 1381, 1921, 2862, 3200, 3357, 4160
MunI CAATTG 3 cut(s) 1742, 2904, 4116
Mva1269I GAATGC 7 cut(s) 2357, 2877, 3158, 3688, 3720, 4037, 4097
MvaI CCWGG 6 cut(s) 1381, 1921, 2862, 3200, 3357, 4160
MvnI CGCG 1 cut(s) 2515
NcoI CCATGG 3 cut(s) 1532, 1870, 4171
NdeI CATATG 3 cut(s) 703, 1903, 2986
NlaIV GGNNCC 4 cut(s) 768, 1789, 1917, 2214
NmuCI GTSAC 4 cut(s) 556, 1765, 2042, 3562
NsiI ATGCAT 2 cut(s) 107, 3722
NspI RCATGY 1 cut(s) 2309
NspV TTCGAA 1 cut(s) 812
OliI CACNNNNGTG 2 cut(s) 539, 2750
PagI TCATGA 3 cut(s) 166, 622, 1678
PciI ACATGT 1 cut(s) 2305
PciSI GCTCTTC 1 cut(s) 1731
PcsI WCGNNNNNNNCGW 1 cut(s) 3076
PctI GAATGC 7 cut(s) 2357, 2877, 3158, 3688, 3720, 4037, 4097
PflFI GACNNNGTC 2 cut(s) 3200, 4154
PleI GAGTC 5 cut(s) 743, 1762, 2227, 2416, 3790
PmaCI CACGTG 1 cut(s) 3992
PmlI CACGTG 1 cut(s) 3992
PpsI GAGTC 5 cut(s) 743, 1762, 2227, 2416, 3790
Ppu21I YACGTR 2 cut(s) 1943, 3992
PpuMI RGGWCCY 4 cut(s) 1915, 3172, 4015, 4156
PscI ACATGT 1 cut(s) 2305
PshAI GACNNNNGTC 1 cut(s) 2407
PshBI ATTAAT 3 cut(s) 1542, 1550, 2030
PsiI TTATAA 2 cut(s) 284, 2124
Psp124BI GAGCTC 1 cut(s) 3469
Psp5II RGGWCCY 4 cut(s) 1915, 3172, 4015, 4156
Psp6I CCWGG 6 cut(s) 1379, 1919, 2860, 3198, 3355, 4158
PspCI CACGTG 1 cut(s) 3992
PspFI CCCAGC 1 cut(s) 2962
PspGI CCWGG 6 cut(s) 1379, 1919, 2860, 3198, 3355, 4158
PspN4I GGNNCC 4 cut(s) 768, 1789, 1917, 2214
PspPI GGNCC 9 cut(s) 767, 850, 1915, 2213, 2757, 3172, 4015, 4156, 4162
PspPPI RGGWCCY 4 cut(s) 1915, 3172, 4015, 4156
PstNI CAGNNNCTG 1 cut(s) 3662
PsuI RGATCY 5 cut(s) 261, 527, 2893, 3320, 3763
PsyI GACNNNGTC 2 cut(s) 3200, 4154
PvuII CAGCTG 4 cut(s) 452, 1631, 2330, 3593
RseI CAYNNNNRTG 7 cut(s) 539, 706, 1448, 1531, 2750, 3706, 3725
SacI GAGCTC 1 cut(s) 3469
SapI GCTCTTC 1 cut(s) 1731
Sau96I GGNCC 9 cut(s) 767, 850, 1915, 2213, 2757, 3172, 4015, 4156, 4162
ScaI AGTACT 2 cut(s) 1370, 3352
SchI GAGTC 5 cut(s) 743, 1762, 2227, 2417, 3790
ScrFI CCNGG 6 cut(s) 1381, 1921, 2862, 3200, 3357, 4160
SduI GDGCHC 6 cut(s) 15, 1608, 2805, 3469, 3775, 3997
SexAI ACCWGGT 2 cut(s) 2860, 3198
SfcI CTRYAG 3 cut(s) 1023, 1896, 3055
SfuI TTCGAA 1 cut(s) 812
SinI GGWCC 7 cut(s) 767, 1915, 2213, 3172, 4015, 4156, 4162
SmiMI CAYNNNNRTG 7 cut(s) 539, 706, 1448, 1531, 2750, 3706, 3725
SmlI CTYRAG 4 cut(s) 961, 1571, 3470, 3568
SmoI CTYRAG 4 cut(s) 961, 1571, 3470, 3568
SpeI ACTAGT 1 cut(s) 2749
SsiI CCGC 8 cut(s) 406, 764, 1276, 1576, 3488, 3694, 4038, 4199
SspI AATATT 2 cut(s) 820, 2687
SstI GAGCTC 1 cut(s) 3469
StyD4I CCNGG 6 cut(s) 1379, 1919, 2860, 3198, 3355, 4158
StyI CCWWGG 3 cut(s) 1532, 1870, 4171
TaiI ACGT 4 cut(s) 1945, 2157, 3486, 3994
TaqI TCGA 7 cut(s) 383, 510, 812, 2019, 2850, 3100, 4253
TatI WGTACW 5 cut(s) 425, 1368, 2239, 3350, 3710
TseFI GTSAC 4 cut(s) 556, 1765, 2042, 3562
Tsp45I GTSAC 4 cut(s) 556, 1765, 2042, 3562
TspGWI ACGGA 2 cut(s) 43, 1378
Tth111I GACNNNGTC 2 cut(s) 3200, 4154
Vha464I CTTAAG 1 cut(s) 961
VneI GTGCAC 3 cut(s) 2801, 3771, 3993
VpaK11BI GGWCC 7 cut(s) 767, 1915, 2213, 3172, 4015, 4156, 4162
VspI ATTAAT 3 cut(s) 1542, 1550, 2030
XagI CCTNNNNNAGG 1 cut(s) 3537
XapI RAATTY 6 cut(s) 246, 1522, 1747, 2375, 2734, 3551
XceI RCATGY 1 cut(s) 2309
XcmI CCANNNNNNNNNTGG 2 cut(s) 1179, 1917
XmiI GTMKAC 1 cut(s) 1115
ZraI GACGTC 1 cut(s) 2155
ZrmI AGTACT 2 cut(s) 1370, 3352
Zsp2I ATGCAT 2 cut(s) 107, 3722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.