Rw2G029350

ABC transporter C family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002799
Physical Location & Seq
Forward (+)
47439173 .. 47459822
20650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G029350.1

Sequence Viewer

Length: 4422 bp
ATGGACGATTTCAGGAACCTGATCTGCCCTAATTCTCCCTCCGTGTGGGACGGGAATGGACTTTCCAAGTGCTACGGCAACATATATCCTTTTCAACTCCTTCTGTTTTGGTCTTTTGGTCCTAATATAGTCACTCTTGCTGTGATTTTCGTACTCGGAATCACTCCAAGGACCGGCAGAAGAGCCTGGAGGATGAACTTTTCCGAGAGGTTTTTCTTGCACGTGCTTCCTGCAGTCGGAGCTTGTTTAGCATTTTTCGACATTGCTTTACTTTTAAAGAAAGGTCGTGATGGTGTCTTGGTTGCAAAACATGAATGGTTGTTCAGATGTTCCCAGTTTGCTGTCTGGACAGTTGTCATACTCTTCTCAAGGTGTTTCAATGCTTGCCATATTTTTGGCAATCGCATCTTGTGTTTCTGGTGGCTTGTAAAAATGCTTTTGGCAACACTTCATTTGTTCACAACATATCCTTCATTCAAGGTCTTGTTATCTCTTAAGGAGATTGGTACTGTTTCATTGGACGTCATTTTTGGTATATCAATTAGCATCATCAGAATGAAGAGGGCATCATCCAACAGAAGTTCAATGGAAGATTCACTACTTTCTGCTGACATGGACCTCGAGGAAGGTTCTCTCAAGGACTCTGGCGACAATCAGAGCTATTTCAATCTTATGACATTCAGATCGATCACTTCTGTGATGAGTCATGGTGTCACAAAGCAGCTTGAATTTGAAGATTTGCTTCAACTTCCCACTGATATGGACCCTTGTTCCTGTCATGATGCATTATTTAGCTGTTGGAAATCCCAACAGAGTAGTTGCCCTGATCCATCCCTGTTTAGGGCAATCTGTTCTGCATATGGATGGCCATACGTTCATCTCGGCTTGTTAAAGGTACTTAATGATTCTGTTGGTTTTGCTGGACCGTTGCTTCTCAATAAGCTAATTCGCTTCCTTCAACAAGGTTCGCAGAATTTGGATGGCTATGTTCTTGCTGTATCCTTGGGCCTCATATCTATCTTTAAATCGTTTTTGGATACACAATATACTTTTCATCTTTCAAAATTGAAGTTAAAGCTACGATCTAGTATCATGACAGTTATATATCAGAAGTGCCTCTGTGTCAACCTAGCAGAGCGATCAAAGTTTACCGAGGGGGAAATTCAGACATTCATGGCCATAGATTCTGATCGCATTGTCAACTTAAGTAACACTCTCCATGATATGTGGAGCTTGCCTTTACAAATTGGGGTGGCTTTGTTCCTTTTGTATACACAAGTCAAATTTGCATTTGTTTCTGGGCTTGCAATTACCATCGCATTAATACCAGTGAATAAATGGATATCTACATTGATTGCAAGAGCTACAGTGAAAATGATGAAGCAGAAGGATGAGAGGATCAGAAGGACGGGGGAACTTTTGACATATATCCGCACTTTGAAAATGTATGGTTGGGAGCTCTTGTTTTCTAGCTGGTTGATGGAGACAAGATTATCAGAAGTTATGCACTTAACTACACGGAAGTACTTGGATGCCTGGTGTGTATTTTTTTGGGCCACAACACCAACTCTTTTTTCTCTTTGTACATTTGGACTTTTCACATTGATGGGGCATCAACTTGATGCTGCAACGGTCTTCACTTGTGTTGCTCTGTTTAATACTTTGATATCTCCTCTAAACTCATTTCCGTGGGTCATTAATGGGTTAATTGATGCTGTCATATCTGTCAGACGGTTGAGCAGGTTCCTGTCTTGCTCTGAGCGCAAGTCAAAGCTGGAAAAGACAGCTGATGCATCTTCACCATATTTCTCCAATGATCAGTGTGAATTTACCTTTGAAGATATGGCTGTTGTGTTTGATGATTCATCTTGTTCCTGGTCAAGCAGCGATGAAAAGGAATTGAATTTGGTGCTGAATCATGTAACTCTAGGTATTCCAAAGGGTTCCTTCATTGCAGTAATTGGAGAGGTTGGTTCAGGTAAATCATCATTGTTGAATTCCATATTGGGGGAAATGCAACTTGTCCATGGATCAGTATATTCTTGTGGATCTATAGCATATGTACCACAGGTCCCATGGATTCTGTCTGGAACAATCCGTGACAATATACTGTTTGGAAAGCAATACGATCCCAAAAGATACACAGATACTTTAGAGGCAAGTGCTTTAGATGTTGATATTTCAATAATGGTTGGAGGTGACATGGCTTATATTGGAGAAAAAGGAGTCAACTTGTCAGGTGGACAGAGAGCTCGAATTGCTCTGGCAAGGGCTATTTATAATGGCTCCGATATGTTTATTCTTGATGATGTCCTCAGTGCAGTTGATGCAAGAGTTGCTCGTTGTATTTTATACAATGCAATTTTGGGTCCACTAATGAAAAAGCAAACTCGTGTGCTTTGTACGCATAACGTTCAGGCAATATCTTCTGCTGATACAATTGTGGTTATGGAAAAAGGACATGTAAAGTGGGTGGGACCTTCTGCTTGTTTGCCAGCTTCTTCATATTCAGCATTCTCTCAACTGAATGAATTTGATAAATTCTTACTGAATGAAGGAAAAGAGTGCAATGTACCTGCAGATTCTCTCACAAAAGACCAACAAAACCTTCCCTTAGAGAAAGATATTGTGCCTGCTTCAGAGGGAAGACAAGATATTATTGAAGTTGAGGCACGGAAAGAGGGAAAAGTAGAACTTTCTGTCTACAAGAATTATGCCACGTTTACTGGTTGGTTCATTTCGGTTGTAATATTTCTATCAGCAATCCTAATGCAAGCTTCTCGTAATGGAAATGATCTGTGGCTGTCATATTGGGTTGATACAACAGGAAAGAGTCAGAAAGGATACTCTACATCCTTTTATCTGGTAATACTGTGCATCTTTTGTACTGCAAATTCAGTTCTTACATTAGTGAGGGCCTTCTCATTTGCATATGGCGGCGTACGAGCTGCAGTTAAGGTGCATGATACACTGCTGAAAAGGCTTATCAATGCACCTGTTCAATTCTTTGATCAGACACCAGGTGGAAGGATACTAAACAGGCTATCTTCAGATCTATACACAATTGATGATTCTCTTCCCTTCATTCTCAACATTCTCCTAGCTAATTTCGTTGGTCTTCTGGGAATTGCAATAGTTTTGTCATATGTTCAGGTCTTCTTCTTGCTTTTGCTATTGCCGTTTTGGTATATCTACACCAAACTTCAGTTCTTCTACAGGTCAACATCTCGAGAATTGCGAAGGCTGGACAGTGTGTCTCGGTCTCCCATATATACATCCTTCACAGAGACACTTGATGGATCATCAACTATACGGGCATTTAAGTCTGAGGACTTCTTTTTGGCCAGATTCACTGACCAGGTGAAATTGTATCAACAAACTTCCTACACAGAGTTAAATGCAAGTTTGTGGCTTTCCCTACGTCTTCAGTTGTCAGCAGCATTTATCATCTCATTTGTTGCTATTATGGCTGTTCTTGGATCTCATGGTGGATTACCAATCGGTTTCAGTACCCCAGGGCTGGTTGGTTTGGCTCTATCTTATGCAGCTCCGGTTGTAAATTTACTGGGAAGCTTTTTGACAAGTTTCACTGAAACAGAGAAGGAAATGGTTTCCATTGAAAGAGCTCTTGAGTATATGGAGGTTCCTCAAGAAGAAGTTGATGGACTTCAATCTTTAAACTGCAATTGGCCATTTCAAGGACGGATTGAATTCCAAAATGTTACCTTGAGATACAAGCCATCTTTTCCAGCTGCACTTTGTGATATTAGTTTTACTATTGACGGAGGGATGCAGGTTGGTATTGTTGGAAGGACAGGAGCTGGAAAATCTAGTGTTTTGAATGCCCTTTTCCGCCTCACACCAATATGCACAGGATGTATTCTTGTGGATGATATAAACATAGCTAATGCTCCTATCAGGGATCTTCGTGGGCATTTCTCTGTTGTTCCTCAGACTCCATTTTTATTTGAAGGATCATTAAGGGACAATCTGGATCCATTCAGGCTGAGTGATGACTATAAAATTTGGAAGGCTTTGGAGAGGTGCCATGTCAAAGAGGAAGTAGAAGCAGCAGGAGGTTTAGATATTCACCTAAGCGAATCTAGGATGTCCTTTTCTGTAGGGCAACGGCAGCTTCTTTGCCTTGCACGTGCACTGCTCAAATCTTCAAAGGTGCTTTGTTTGGATGAATGCACAGCTAATGTGGACACTCAAACAGCATCTACCTTACAAAAAACCATATCTAGTGAATGCAGTGGCATGACAGTTATCACAATTGCTCATCGTATTTCCACCATTCTGAATATGGATAATGTCCTGATCCTCGACCATGGTATCCTGGTTGAACAAGGAAATCCGCAGGCTCTCCTACAAAATGAGTTCTCCAGATTTTCCAGTTTTGCTAAAGCATCTACAATGTGA

Protein Analysis

1473

Amino Acids

164.47

Weight (kDa)

7.03

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 295 - 559 2.7e-20 ABC transporter transmembrane region
ABC_tran PF00005 638 - 771 4.9e-16 ABC transporter
ABC_membrane PF00664 916 - 1164 6.6e-43 ABC transporter transmembrane region
ABC_tran PF00005 1255 - 1400 2e-24 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2280
AatII GACGTC 1 cut(s) 525
AbsI CCTCGAGG 1 cut(s) 620
Acc36I ACCTGC 3 cut(s) 1731, 2584, 3784
AccB1I GGYRCC 1 cut(s) 4044
AccI GTMKAC 2 cut(s) 1271, 2703
AciI CCGC 4 cut(s) 1432, 2937, 3853, 4358
AclI AACGTT 1 cut(s) 2412
AcoI YGGCCR 4 cut(s) 866, 1176, 3342, 3689
AcuI CTGAAG 4 cut(s) 2622, 3033, 3188, 3410
AcvI CACGTG 2 cut(s) 223, 4151
AcyI GRCGYC 1 cut(s) 522
AdeI CACNNNGTG 2 cut(s) 3023, 3761
AfiI CCNNNNNNNGG 8 cut(s) 45, 173, 236, 840, 841, 2007, 3520, 3698
AflII CTTAAG 2 cut(s) 494, 1204
AflIII ACRYGT 1 cut(s) 2461
AhdI GACNNNNNGTC 1 cut(s) 3253
AjnI CCWGG 7 cut(s) 185, 1535, 1874, 3019, 3357, 3514, 4338
AjuI GAANNNNNNNTTGG 8 cut(s) 1558, 1590, 1988, 2020, 2592, 2624, 3191, 3223
AleI CACNNNNGTG 1 cut(s) 695
Alw21I GWGCWC 4 cut(s) 1461, 2254, 3628, 4156
Alw26I GTCTC 4 cut(s) 1478, 3261, 3267, 3281
Alw44I GTGCAC 1 cut(s) 4152
AlwNI CAGNNNCTG 1 cut(s) 3821
Ama87I CYCGRG 2 cut(s) 620, 3228
AoxI GGCC 7 cut(s) 866, 1006, 1176, 1554, 2916, 3342, 3689
ApaLI GTGCAC 1 cut(s) 4152
ApeKI GCWGC 9 cut(s) 721, 1625, 1884, 2948, 3437, 3545, 3752, 4070, 4132
ArsI GACNNNNNNTTYG 2 cut(s) 512, 544
AseI ATTAAT 2 cut(s) 1322, 1698
AspLEI GCGC 1 cut(s) 1764
AsuHPI GGTGA 4 cut(s) 1791, 2210, 3373, 4082
AvaI CYCGRG 2 cut(s) 620, 3228
AvaII GGWCC 8 cut(s) 119, 171, 616, 763, 923, 2071, 2369, 2477
BaeGI GKGCMC 1 cut(s) 4156
BaeI ACNNNNGTAYC 4 cut(s) 2427, 2460, 4318, 4351
BalI TGGCCA 4 cut(s) 868, 1178, 3344, 3691
BamHI GGATCC 1 cut(s) 3994
BanI GGYRCC 1 cut(s) 4044
BanII GRGCYC 3 cut(s) 1461, 2254, 3628
BarI GAAGNNNNNNTAC 2 cut(s) 582, 614
BauI CACGAG 1 cut(s) 2391
BbrPI CACGTG 2 cut(s) 223, 4151
BbsI GAAGAC 5 cut(s) 1627, 2653, 3110, 3148, 3416
Bbv12I GWGCWC 4 cut(s) 1461, 2254, 3628, 4156
BbvI GCAGC 9 cut(s) 733, 1612, 1896, 2935, 3449, 3557, 3739, 4082, 4144
BceAI ACGGC 3 cut(s) 91, 3163, 4145
BcgI CGANNNNNNTGC 2 cut(s) 2762, 2796
BciT130I CCWGG 7 cut(s) 187, 1537, 1876, 3021, 3359, 3516, 4340
BciVI GTATCC 5 cut(s) 1009, 1030, 2837, 3024, 4346
BclI TGATCA 2 cut(s) 1816, 3010
BcoDI GTCTC 4 cut(s) 1478, 3261, 3267, 3281
BfaI CTAG 8 cut(s) 1086, 1130, 1470, 1928, 3101, 3831, 4104, 4245
BfmI CTRYAG 7 cut(s) 231, 1365, 2052, 2577, 2949, 3214, 4119
BfrI CTTAAG 2 cut(s) 494, 1204
BfuAI ACCTGC 3 cut(s) 1731, 2584, 3784
BfuI GTATCC 5 cut(s) 1009, 1030, 2837, 3024, 4346
BglII AGATCT 1 cut(s) 3052
BmcAI AGTACT 1 cut(s) 1526
Bme1390I CCNGG 7 cut(s) 187, 1537, 1876, 3021, 3359, 3516, 4340
Bme18I GGWCC 8 cut(s) 119, 171, 616, 763, 923, 2071, 2369, 2477
BmeRI GACNNNNNGTC 1 cut(s) 3253
BmeT110I CYCGRG 2 cut(s) 620, 3228
BmrFI CCNGG 7 cut(s) 187, 1537, 1876, 3021, 3359, 3516, 4340
BmrI ACTGGG 2 cut(s) 328, 3575
BmuI ACTGGG 2 cut(s) 328, 3575
BoxI GACNNNNGTC 1 cut(s) 353
BpiI GAAGAC 5 cut(s) 1627, 2653, 3110, 3148, 3416
BpmI CTGGAG 2 cut(s) 208, 4369
Bpu10I CCTNAGC 1 cut(s) 4094
BpuEI CTTGAG 5 cut(s) 352, 620, 3633, 3650, 3748
Bsa29I ATCGAT 1 cut(s) 686
BsaAI YACGTR 2 cut(s) 223, 4151
BsaBI GATNNNNATC 1 cut(s) 1188
BsaHI GRCGYC 1 cut(s) 522
BsaI GGTCTC 1 cut(s) 3267
BsaJI CCNNGG 9 cut(s) 167, 1002, 1152, 1688, 2026, 2075, 3514, 3515, 4330
BsaWI WCCGGW 1 cut(s) 3550
BsaXI ACNNNNNCTCC 2 cut(s) 1448, 1478
Bsc4I CCNNNNNNNGG 8 cut(s) 45, 173, 236, 840, 841, 2007, 3520, 3698
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 5 cut(s) 334, 1328, 2731, 3570, 4395
Bse3DI GCAATG 3 cut(s) 261, 1950, 2575
Bse8I GATNNNNATC 1 cut(s) 1188
BseBI CCWGG 7 cut(s) 187, 1537, 1876, 3021, 3359, 3516, 4340
BseCI ATCGAT 1 cut(s) 686
BseDI CCNNGG 9 cut(s) 167, 1002, 1152, 1688, 2026, 2075, 3514, 3515, 4330
BseJI GATNNNNATC 1 cut(s) 1188
BseLI CCNNNNNNNGG 8 cut(s) 45, 173, 236, 840, 841, 2007, 3520, 3698
BseMI GCAATG 3 cut(s) 261, 1950, 2575
BseMII CTCAG 5 cut(s) 1749, 2329, 3318, 3965, 3998
BseNI ACTGG 5 cut(s) 334, 1328, 2731, 3570, 4395
BseRI GAGGAG 1 cut(s) 1662
BseSI GKGCMC 1 cut(s) 4156
BseXI GCAGC 9 cut(s) 733, 1612, 1896, 2935, 3449, 3557, 3739, 4082, 4144
BsgI GTGCAG 2 cut(s) 2340, 3738
BshFI GGCC 7 cut(s) 868, 1008, 1178, 1556, 2918, 3344, 3691
BshNI GGYRCC 1 cut(s) 4044
BshVI ATCGAT 1 cut(s) 686
BsiHKAI GWGCWC 4 cut(s) 1461, 2254, 3628, 4156
BsiHKCI CYCGRG 2 cut(s) 620, 3228
BsiSI CCGG 2 cut(s) 174, 3551
BsiWI CGTACG 1 cut(s) 2941
BslFI GGGAC 4 cut(s) 62, 2057, 2490, 3998
BslI CCNNNNNNNGG 8 cut(s) 45, 173, 236, 840, 841, 2007, 3520, 3698
BsmAI GTCTC 4 cut(s) 1478, 3261, 3267, 3281
BsmFI GGGAC 4 cut(s) 62, 2057, 2490, 3998
BsmI GAATGC 4 cut(s) 2513, 3847, 4196, 4256
BsnI GGCC 7 cut(s) 868, 1008, 1178, 1556, 2918, 3344, 3691
Bso31I GGTCTC 1 cut(s) 3267
BsoBI CYCGRG 2 cut(s) 620, 3228
Bsp1286I GDGCHC 4 cut(s) 1461, 2254, 3628, 4156
Bsp1407I TGTACA 1 cut(s) 1583
Bsp19I CCATGG 3 cut(s) 2026, 2075, 4330
BspACI CCGC 4 cut(s) 1432, 2937, 3853, 4358
BspANI GGCC 7 cut(s) 868, 1008, 1178, 1556, 2918, 3344, 3691
BspCNI CTCAG 5 cut(s) 1750, 2328, 3319, 3964, 3999
BspDI ATCGAT 1 cut(s) 686
BspHI TCATGA 2 cut(s) 778, 1092
BspMAI CTGCAG 3 cut(s) 235, 2581, 2953
BspMI ACCTGC 3 cut(s) 1731, 2584, 3784
BspQI GCTCTTC 1 cut(s) 175
BspT107I GGYRCC 1 cut(s) 4044
BspTI CTTAAG 2 cut(s) 494, 1204
BspTNI GGTCTC 1 cut(s) 3267
BsrDI GCAATG 3 cut(s) 261, 1950, 2575
BsrFI RCCGGY 1 cut(s) 173
BsrGI TGTACA 1 cut(s) 1583
BsrI ACTGG 5 cut(s) 334, 1328, 2731, 3570, 4395
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 9 cut(s) 167, 1002, 1152, 1688, 2026, 2075, 3514, 3515, 4330
BssNAI GTATAC 1 cut(s) 1272
BssNI GRCGYC 1 cut(s) 522
BssSI CACGAG 1 cut(s) 2391
BssT1I CCWWGG 5 cut(s) 167, 1002, 2026, 2075, 4330
Bst1107I GTATAC 1 cut(s) 1272
Bst2BI CACGAG 1 cut(s) 2391
Bst2UI CCWGG 7 cut(s) 187, 1537, 1876, 3021, 3359, 3516, 4340
Bst6I CTCTTC 4 cut(s) 175, 368, 554, 3081
BstACI GRCGYC 1 cut(s) 522
BstAFI CTTAAG 2 cut(s) 494, 1204
BstAPI GCANNNNNTGC 2 cut(s) 2327, 2336
BstAUI TGTACA 1 cut(s) 1583
BstBAI YACGTR 2 cut(s) 223, 4151
BstC8I GCNNGC 7 cut(s) 385, 1235, 1305, 2496, 2634, 2775, 4362
BstDEI CTNAG 7 cut(s) 1758, 2315, 2614, 3327, 3951, 4007, 4094
BstDSI CCRYGG 4 cut(s) 1688, 2026, 2075, 4330
BstHHI GCGC 1 cut(s) 1764
BstMAI GTCTC 4 cut(s) 1478, 3261, 3267, 3281
BstNI CCWGG 7 cut(s) 187, 1537, 1876, 3021, 3359, 3516, 4340
BstNSI RCATGY 1 cut(s) 2465
BstPAI GACNNNNGTC 1 cut(s) 353
BstSCI CCNGG 7 cut(s) 185, 1535, 1874, 3019, 3357, 3514, 4338
BstSFI CTRYAG 7 cut(s) 231, 1365, 2052, 2577, 2949, 3214, 4119
BstSLI GKGCMC 1 cut(s) 4156
BstV1I GCAGC 9 cut(s) 733, 1612, 1896, 2935, 3449, 3557, 3739, 4082, 4144
BstV2I GAAGAC 5 cut(s) 1627, 2653, 3110, 3148, 3416
BstX2I RGATCY 5 cut(s) 2048, 3052, 3479, 3922, 3994
BstXI CCANNNNNNTGG 2 cut(s) 395, 760
BstYI RGATCY 5 cut(s) 2048, 3052, 3479, 3922, 3994
BstZ17I GTATAC 1 cut(s) 1272
Bsu15I ATCGAT 1 cut(s) 686
BsuI GTATCC 5 cut(s) 1009, 1030, 2837, 3024, 4346
BsuRI GGCC 7 cut(s) 868, 1008, 1178, 1556, 2918, 3344, 3691
BsuTUI ATCGAT 1 cut(s) 686
BtgI CCRYGG 4 cut(s) 1688, 2026, 2075, 4330
BtgZI GCGATG 2 cut(s) 1300, 1902
BtsI GCAGTG 3 cut(s) 2969, 4154, 4261
BveI ACCTGC 3 cut(s) 1731, 2584, 3784
Cac8I GCNNGC 7 cut(s) 385, 1235, 1305, 2496, 2634, 2775, 4362
CaiI CAGNNNCTG 1 cut(s) 3821
CciI TCATGA 2 cut(s) 778, 1092
CfoI GCGC 1 cut(s) 1764
Cfr10I RCCGGY 1 cut(s) 173
ClaI ATCGAT 1 cut(s) 686
CsiI ACCWGGT 2 cut(s) 3019, 3357
DdeI CTNAG 7 cut(s) 1758, 2315, 2614, 3327, 3951, 4007, 4094
DraI TTTAAA 3 cut(s) 276, 1024, 3678
DraIII CACNNNGTG 2 cut(s) 3023, 3761
DriI GACNNNNNGTC 1 cut(s) 3253
EaeI YGGCCR 4 cut(s) 866, 1176, 3342, 3689
Eam1104I CTCTTC 4 cut(s) 175, 368, 554, 3081
Eam1105I GACNNNNNGTC 1 cut(s) 3253
EarI CTCTTC 4 cut(s) 175, 368, 554, 3081
EciI GGCGGA 1 cut(s) 3842
Ecl136II GAGCTC 3 cut(s) 1459, 2252, 3626
Eco130I CCWWGG 5 cut(s) 167, 1002, 2026, 2075, 4330
Eco24I GRGCYC 3 cut(s) 1461, 2254, 3628
Eco31I GGTCTC 1 cut(s) 3267
Eco32I GATATC 2 cut(s) 1344, 1668
Eco47I GGWCC 8 cut(s) 119, 171, 616, 763, 923, 2071, 2369, 2477
Eco53kI GAGCTC 3 cut(s) 1459, 2252, 3626
Eco57I CTGAAG 4 cut(s) 2622, 3033, 3188, 3410
Eco72I CACGTG 2 cut(s) 223, 4151
Eco88I CYCGRG 2 cut(s) 620, 3228
EcoICRI GAGCTC 3 cut(s) 1459, 2252, 3626
EcoO109I RGGNCCY 3 cut(s) 2071, 2477, 2916
EcoRI GAATTC 2 cut(s) 1996, 3710
EcoRII CCWGG 7 cut(s) 185, 1535, 1874, 3019, 3357, 3514, 4338
EcoRV GATATC 2 cut(s) 1344, 1668
EcoT14I CCWWGG 5 cut(s) 167, 1002, 2026, 2075, 4330
EcoT22I ATGCAT 2 cut(s) 787, 1795
EcoT38I GRGCYC 3 cut(s) 1461, 2254, 3628
ErhI CCWWGG 5 cut(s) 167, 1002, 2026, 2075, 4330
FalI AAGNNNNNCTT 6 cut(s) 726, 758, 1931, 1963, 2679, 2711
FaqI GGGAC 4 cut(s) 62, 2057, 2490, 3998
FauNDI CATATG 4 cut(s) 859, 2059, 2932, 3145
FbaI TGATCA 2 cut(s) 1816, 3010
FblI GTMKAC 2 cut(s) 1271, 2703
FriOI GRGCYC 3 cut(s) 1461, 2254, 3628
FspBI CTAG 8 cut(s) 1086, 1130, 1470, 1928, 3101, 3831, 4104, 4245
GlaI GCGC 1 cut(s) 1763
GsuI CTGGAG 2 cut(s) 208, 4369
HaeIII GGCC 7 cut(s) 868, 1008, 1178, 1556, 2918, 3344, 3691
HapII CCGG 2 cut(s) 174, 3551
HhaI GCGC 1 cut(s) 1764
Hin1I GRCGYC 1 cut(s) 522
Hin6I GCGC 1 cut(s) 1762
HinP1I GCGC 1 cut(s) 1762
HincII GTYRAC 4 cut(s) 1126, 1201, 2230, 3222
HindII GTYRAC 4 cut(s) 1126, 1201, 2230, 3222
HindIII AAGCTT 2 cut(s) 2775, 3571
HpaII CCGG 2 cut(s) 174, 3551
HphI GGTGA 4 cut(s) 1791, 2210, 3373, 4082
HpyCH4IV ACGT 7 cut(s) 222, 522, 873, 2412, 2720, 3421, 4150
HpyF3I CTNAG 7 cut(s) 1758, 2315, 2614, 3327, 3951, 4007, 4094
HpySE526I ACGT 7 cut(s) 222, 522, 873, 2412, 2720, 3421, 4150
Hsp92I GRCGYC 1 cut(s) 522
HspAI GCGC 1 cut(s) 1762
Ksp22I TGATCA 2 cut(s) 1816, 3010
LguI GCTCTTC 1 cut(s) 175
LmnI GCTCC 7 cut(s) 239, 1230, 1456, 2291, 3553, 3818, 3916
Lsp1109I GCAGC 9 cut(s) 733, 1612, 1896, 2935, 3449, 3557, 3739, 4082, 4144
MabI ACCWGGT 2 cut(s) 3019, 3357
MaeI CTAG 8 cut(s) 1086, 1130, 1470, 1928, 3101, 3831, 4104, 4245
MaeII ACGT 7 cut(s) 222, 522, 873, 2412, 2720, 3421, 4150
MaeIII GTNAC 7 cut(s) 130, 712, 1208, 1921, 2099, 2198, 3721
MfeI CAATTG 4 cut(s) 2439, 3063, 3685, 4275
MflI RGATCY 5 cut(s) 2048, 3052, 3479, 3922, 3994
MhlI GDGCHC 4 cut(s) 1461, 2254, 3628, 4156
MlsI TGGCCA 4 cut(s) 868, 1178, 3344, 3691
MluNI TGGCCA 4 cut(s) 868, 1178, 3344, 3691
MlyI GAGTC 5 cut(s) 635, 712, 2235, 2842, 3949
MmeI TCCRAC 5 cut(s) 217, 597, 779, 2173, 3787
Mox20I TGGCCA 4 cut(s) 868, 1178, 3344, 3691
Mph1103I ATGCAT 2 cut(s) 787, 1795
MscI TGGCCA 4 cut(s) 868, 1178, 3344, 3691
MslI CAYNNNNRTG 7 cut(s) 554, 695, 758, 862, 1604, 1687, 3865
Msp20I TGGCCA 4 cut(s) 868, 1178, 3344, 3691
MspA1I CMGCKG 2 cut(s) 1787, 3752
MspCI CTTAAG 2 cut(s) 494, 1204
MspI CCGG 2 cut(s) 174, 3551
MspR9I CCNGG 7 cut(s) 187, 1537, 1876, 3021, 3359, 3516, 4340
MunI CAATTG 4 cut(s) 2439, 3063, 3685, 4275
Mva1269I GAATGC 4 cut(s) 2513, 3847, 4196, 4256
MvaI CCWGG 7 cut(s) 187, 1537, 1876, 3021, 3359, 3516, 4340
NcoI CCATGG 3 cut(s) 2026, 2075, 4330
NdeI CATATG 4 cut(s) 859, 2059, 2932, 3145
NmeAIII GCCGAG 1 cut(s) 861
NmuCI GTSAC 4 cut(s) 130, 712, 2099, 2198
NsiI ATGCAT 2 cut(s) 787, 1795
NspI RCATGY 1 cut(s) 2465
OliI CACNNNNGTG 1 cut(s) 695
PaeR7I CTCGAG 2 cut(s) 620, 3228
PagI TCATGA 2 cut(s) 778, 1092
PasI CCCWGGG 1 cut(s) 3515
PciI ACATGT 1 cut(s) 2461
PciSI GCTCTTC 1 cut(s) 175
PcsI WCGNNNNNNNCGW 1 cut(s) 3235
PctI GAATGC 4 cut(s) 2513, 3847, 4196, 4256
Pfl23II CGTACG 1 cut(s) 2941
PleI GAGTC 5 cut(s) 635, 711, 2234, 2841, 3949
PmaCI CACGTG 2 cut(s) 223, 4151
PmlI CACGTG 2 cut(s) 223, 4151
PpsI GAGTC 5 cut(s) 635, 711, 2234, 2841, 3949
Ppu21I YACGTR 2 cut(s) 223, 4151
PpuMI RGGWCCY 2 cut(s) 2071, 2477
PscI ACATGT 1 cut(s) 2461
PshAI GACNNNNGTC 1 cut(s) 353
PshBI ATTAAT 2 cut(s) 1322, 1698
PsiI TTATAA 1 cut(s) 2280
Psp124BI GAGCTC 3 cut(s) 1461, 2254, 3628
Psp1406I AACGTT 1 cut(s) 2412
Psp5II RGGWCCY 2 cut(s) 2071, 2477
Psp6I CCWGG 7 cut(s) 185, 1535, 1874, 3019, 3357, 3514, 4338
PspCI CACGTG 2 cut(s) 223, 4151
PspGI CCWGG 7 cut(s) 185, 1535, 1874, 3019, 3357, 3514, 4338
PspLI CGTACG 1 cut(s) 2941
PspPPI RGGWCCY 2 cut(s) 2071, 2477
PspXI VCTCGAGB 1 cut(s) 620
PstI CTGCAG 3 cut(s) 235, 2581, 2953
PstNI CAGNNNCTG 1 cut(s) 3821
PsuI RGATCY 5 cut(s) 2048, 3052, 3479, 3922, 3994
PvuII CAGCTG 2 cut(s) 1787, 3752
RseI CAYNNNNRTG 7 cut(s) 554, 695, 758, 862, 1604, 1687, 3865
SacI GAGCTC 3 cut(s) 1461, 2254, 3628
SapI GCTCTTC 1 cut(s) 175
ScaI AGTACT 1 cut(s) 1526
SchI GAGTC 5 cut(s) 635, 712, 2235, 2842, 3949
ScrFI CCNGG 7 cut(s) 187, 1537, 1876, 3021, 3359, 3516, 4340
SduI GDGCHC 4 cut(s) 1461, 2254, 3628, 4156
SexAI ACCWGGT 2 cut(s) 3019, 3357
SfcI CTRYAG 7 cut(s) 231, 1365, 2052, 2577, 2949, 3214, 4119
Sfr274I CTCGAG 2 cut(s) 620, 3228
SinI GGWCC 8 cut(s) 119, 171, 616, 763, 923, 2071, 2369, 2477
SlaI CTCGAG 2 cut(s) 620, 3228
SmiMI CAYNNNNRTG 7 cut(s) 554, 695, 758, 862, 1604, 1687, 3865
SmlI CTYRAG 9 cut(s) 367, 494, 620, 635, 1204, 3228, 3629, 3648, 3727
SmoI CTYRAG 9 cut(s) 367, 494, 620, 635, 1204, 3228, 3629, 3648, 3727
SsiI CCGC 4 cut(s) 1432, 2937, 3853, 4358
SspI AATATT 1 cut(s) 2751
SspMI CTAG 8 cut(s) 1086, 1130, 1470, 1928, 3101, 3831, 4104, 4245
SstI GAGCTC 3 cut(s) 1461, 2254, 3628
StyD4I CCNGG 7 cut(s) 185, 1535, 1874, 3019, 3357, 3514, 4338
StyI CCWWGG 5 cut(s) 167, 1002, 2026, 2075, 4330
TaiI ACGT 7 cut(s) 225, 525, 876, 2415, 2723, 3424, 4153
TaqI TCGA 6 cut(s) 258, 621, 686, 2254, 3229, 4326
TaqII GACCGA 1 cut(s) 3249
TatI WGTACW 3 cut(s) 1524, 1583, 2885
TauI GCSGC 1 cut(s) 2940
TseFI GTSAC 4 cut(s) 130, 712, 2099, 2198
TseI GCWGC 9 cut(s) 721, 1625, 1884, 2948, 3437, 3545, 3752, 4070, 4132
Tsp45I GTSAC 4 cut(s) 130, 712, 2099, 2198
TspGWI ACGGA 7 cut(s) 31, 1534, 1677, 2087, 2689, 3718, 3798
Vha464I CTTAAG 2 cut(s) 494, 1204
VneI GTGCAC 1 cut(s) 4152
VpaK11BI GGWCC 8 cut(s) 119, 171, 616, 763, 923, 2071, 2369, 2477
VspI ATTAAT 2 cut(s) 1322, 1698
XceI RCATGY 1 cut(s) 2465
XcmI CCANNNNNNNNNTGG 3 cut(s) 1335, 2073, 4303
XhoI CTCGAG 2 cut(s) 620, 3228
XmiI GTMKAC 2 cut(s) 1271, 2703
XspI CTAG 8 cut(s) 1086, 1130, 1470, 1928, 3101, 3831, 4104, 4245
ZraI GACGTC 1 cut(s) 523
ZrmI AGTACT 1 cut(s) 1526
Zsp2I ATGCAT 2 cut(s) 787, 1795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.