Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0006243

Physical Location & Seq

Genomic Coordinates Forward (+)

31092112 .. 31110892

Gene Structure 18781 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1243400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3321 bp

ATGAAGCGCTACATCTACATCAATGACAATGAGTCATCCCAGTATCTTTACTGTGACAATCGCATATCAAATAGAAAGTATACTCTGTTGAACTTTCTCCCAAAGAATTTATGGGAACAATTCAGCCGCTTCATGAATCAGTACTTTCTGCTGATTGCATGCCTTCAGCTGTGGTCACTTATTACTCCAGTGAATCCTGCTAGTACATGGGGTCCACTTATCTTCATTTTTGCAGTCTCTGCGACAAAAGAGGCATGGGATGATTACAACCGATACCTCTCAGACAAGAAGGCAAATGGGAAAGAAGTTTGGGTTGTTAGGCAGGGCATCAAGAAACATATAAAAGCACAAGATATTCATGTTGGTAACATAGTCTGGCTCCGAGAGAATGATGAGGTGCCATGTGATCTTGTTTTGATTGGTACCTCTGAAGCACAAGGCTTGTGTTATGTAGAGACTTCAGCACTAGATGGCGAAACTGATCTGAAGACAAGGGTTATACCCCCAACTTGCATGGGGATAGATCTTGAATTGTTGCACAAAATTAAGGGTTTAATTGAGTGTCCCAATCCCGATAAGGACATCAGAAGATTTGATGCAAATCTGCGGTTGTTTCCCCCATTTATTGATAATGACATGTGCCCATTAACGATAAAAAACACACTACTTCAGTCTTGTTACTTGCGGAATACAGAGTGGGCCTGTGGAGTAGCAGTGTACACTGGCAATGAAACCAAGCTAGGTATGAGCAGGGGCATACCTGAGCCAAAGCTTACAGCTGTTGATGCCATGATTGACAAGTTGACTGGAGCTATATTTGTTTTCCAAATAGTGGTGGTGATAGTTCTAGGCGTTGCTGGTAATGTGTGGAAGGATACAGAAGCAAGGAAGCAATGGTATGTTCTATATCCAGAGGAAGGTCCGTGGTACGAACTATTAGTAATTCCTCTTCGATTTGAACTACTTTGTTCCATCATGATACCCATATCAATTAAGGTATCTCTGGACCTTGTGAAGAGCTTGTATGCGAAGTTTATTGATTGGGATAATGAAATGATTGACAGAGAAACGAGTACTCCAGCCCATGCAACAAACACAGCAATAAGTGAAGACCTGGGACAAGTAGAGTACATTTTGACTGACAAAACTGGTACCTTAACCGAAAATAGAATGATATTTAGAAGATGCTGTATCAATGGAATTTTCTATGGCAATGAAAATGGGAATGCCTTAAAAGATGAGGAGCTCATAAATGCTGTTGCCGGTGGCTCTTCTGATGTTATTCGTTTCCTTACAGTTATGGCAATATGTAATACAGTGATACCTATTCAAAGCAAAAGTGGAAGTATCTTGTACAAGGCACAATCTCAAGATGAAGATGCTCTTGTTCATGCTGCTGCTCAGCTACATATGGTTTTTGTCAATAAAAATTCAAATATTCTTGAGATCAAATTTAATGCTTCTACAATTCAATATGAAGCTTTGGAAATTCTGGAGTTCACTTCTGATAGGAAAAGAATGTCAGTAGTGGTGAAGGATTGCCAAAATGGAAGGATTATCCTTTTGTCAAAAGGGGCAGATGAAGCAATTCTCCCTTATGCATGTGCTGGACAGCAAACAAGGACATTTGTTGAAGCGGTTGACCAATATGCTCAATTGGGACTACGTACTCTGTGTCTGGCTTGGCGTGAACTAAAAGAAGAGGAATATCAAGAATGGTCTTTGATGTTCAAAGAGGCTAGCAGCACATTAGTTGATAGGGAGTGGAGAATTGCTGAGGTCTGCCAAAGATTAGAGCATGACTTTGAAGTTCTTGGAGTTACTGCAATAGAGGATCGCCTGCAGGATGGTGTACCAGAGACGATTGAGGCATTGAGAAAAGCTGGCATAAATTTTTGGATGCTGACTGGGGACAAGCAGAACACTGCCATACAGATTGCTCTTTCATGCAATTTAATTTCCCCTGAGCCGAAAGGTCAGCTTTTGTTAATTGATGGCAAAACTGAAGATGAAGTTCGTAGAAGTCTAGAGAGAGTTTTACTTACAATGAGGATAACAACTTCAGAACCTAAGGACGTGGCATTTGCCATCGATGGCTGGTCCCTTGAAATTGCACTTAAGCATTATCGGAAAGCTTTTACTGAATTAGCAATATTGTCAAGGACTGCTATATGTTGTCGTGTGACACCATCACAAAAAGCACAGCTTGTAGAGATTCTAAAATCATGTGACTATAGAACATTGGCTATTGGGGATGGTGGAAATGATGTGAGGATGATACAACAAGCTGACATTGGGGTTGGCATCAGTGGTAGAGAAGGACTGCAAGCAGCGAGGGCAGCTGATTATAGTATTGGGAAGTTTAGGTTTCTGAAAAGACTAATACTTGTGCATGGGCGTTACTCATACAATCGTACAGCATTTTTGTCCCAGTACTCCTTTTATAAATCCCTGGTGATATGTTTCATCCAAATTTTTTTCTCTTTTGTTTCAGGGGTCTCTGGAACCAGTCTTTTCAATTCGGTTAGCTTGATGGCTTATAATGTTTTCTACACTAGTGTCCCTGTTTTAGTCAGTGTTCTTGATAAAGATCTCAGTGAGGATACTGTGATGCAGCATCCACAAATTTTGTTTTACTGCCAAGCGGGGAGGCTTCTGAATCCTAGCACATTTGCAGGATGGTTTGGACGATCCCTTTTTCATGCAATTATTGTGTTTGTGATTAGCATACATGCCTATGCCTATGAAAAAAGTGAAATGGAGGAGATTTCAATGGTTGCTCTCTCCGGATGTATTTGGTTGCAAGCTTTTGTTATGGCATTAGAGACAAATTCCTTTACACTATTGCAACATCTTGCTATATGGGGAAATTTAGCTGCCTTCTATATCATCAACTGGATCTTCAGTGCCATTCCATCATCGGGAATGTATACAATAATGTTTCGCTTGTGTAGACAGCCATCTTATTGGATGACAATATTGCTCATAGTTGGAGCCGGGATGGGTCCGATTCTTGCTCTGAAGTACTTCAGGTATACGTACAGGCCAAGCAAAATCAATACGCTTCAGCAGGCGGAACGATTGGGTGGTCCTATCCTGTCTATTGGGAGCATTGAGCCACAACCCAGAACCATTGAAAAAGATGTTTCTCCATTATCAATAACTCAACCCAAGAACAGAAATCCAATTTTTGAGCCTCTACTATCAGATACACCAAATTCAACTAGAAGGTCTTTCGGTTCAGGCGCACCTTTCGATTTCTTTCAGTCCCAGTCGAGGTTGTCTACATCGAATTACGCAAGAAATTGTAAGGACAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0008150 GO:0009987 GO:0010876 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0033036 GO:0034204 GO:0042623 GO:0043492 GO:0044464 GO:0045332 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase Hydrolase Hydrolase_3 PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1107

Amino Acids

125.11

Weight (kDa)

6.05

Isoelectric Point (pI)

39.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	5 - 70	1e-23	Phospholipid-translocating ATPase N-terminal
E1-E2_ATPase	PF00122	101 - 162	7.1e-07	P-type ATPase actuator domain
Cation_ATPase	PF13246	427 - 615	6.9e-16	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	778 - 1017	2.6e-66	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44240 AT5G44240
fragaria_vesca	FvH4_5g29681 FvH4_5g29681 FvH4_5g29681 FvH4_5g29681 FvH4_5g29681 FvH4_5g29681
malus_domestica	MD09G1243400.v1.1 MD17G1235400.v1.1
prunus_persica	Prupe.3G133300_v2.0.a1 Prupe.3G133300_v2.0.a1 Prupe.3G133300_v2.0.a1
pyrus_communis	pycom09g16200 pycom17g23850
rosa_chinensis	RchiOBHm_Chr7g0223111
rosa_laevigata	RLG00000002001 RLG00000002345
rosa_multiflora	Rmu_sc0006373.1_g000014
rosa_roxburghii	Rroxscaffold_3G00236330
rosa_rugosa	Rorug07G0212900 Rorug07G0212900
rosa_samantha	Rh7AG358200 Rh7BG346300 Rh7CG376500 Rh7DG353600
rosa_wichuraiana	Rw7G030480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2445, 2541
Acc65I	GGTACC	2 cut(s)	422, 1151
AccB1I	GGYRCC	3 cut(s)	397, 422, 1151
AccB7I	CCANNNNNTGG	1 cut(s)	832
AccI	GTMKAC	5 cut(s)	80, 2930, 2953, 3035, 3284
AccIII	TCCGGA	1 cut(s)	2786
AciI	CCGC	6 cut(s)	127, 607, 685, 1637, 2645, 3074
AclWI	GGATC	3 cut(s)	1842, 2685, 2906
AfeI	AGCGCT	1 cut(s)	8
AfiI	CCNNNNNNNGG	4 cut(s)	832, 917, 2921, 3276
AflII	CTTAAG	1 cut(s)	2117
AflIII	ACRYGT	1 cut(s)	636
AhdI	GACNNNNNGTC	1 cut(s)	31
AhlI	ACTAGT	1 cut(s)	2555
AjiI	CACGTC	1 cut(s)	2077
AjnI	CCWGG	2 cut(s)	1113, 2451
Alw21I	GWGCWC	1 cut(s)	1248
Alw26I	GTCTC	5 cut(s)	241, 449, 1853, 2503, 2819
AlwI	GGATC	3 cut(s)	1842, 2685, 2906
AlwNI	CAGNNNCTG	1 cut(s)	239
Aor13HI	TCCGGA	1 cut(s)	2786
Aor51HI	AGCGCT	1 cut(s)	8
AoxI	GGCC	2 cut(s)	699, 3044
ApeKI	GCWGC	7 cut(s)	1394, 1397, 1743, 2330, 2339, 2614, 2876
ArsI	GACNNNNNNTTYG	2 cut(s)	2066, 2098
Asp700I	GAANNNNTTC	2 cut(s)	1587, 3026
Asp718I	GGTACC	2 cut(s)	422, 1151
AspLEI	GCGC	2 cut(s)	9, 3248
AspS9I	GGNCC	7 cut(s)	212, 699, 920, 1006, 2100, 3005, 3089
AsuC2I	CCSGG	1 cut(s)	2998
AsuHPI	GGTGA	3 cut(s)	850, 1543, 2467
AsuNHI	GCTAGC	1 cut(s)	1739
AvaII	GGWCC	6 cut(s)	212, 920, 1006, 2100, 3005, 3089
AxyI	CCTNAGG	1 cut(s)	2070
BaeGI	GKGCMC	1 cut(s)	644
BanI	GGYRCC	3 cut(s)	397, 422, 1151
BanII	GRGCYC	1 cut(s)	1248
BarI	GAAGNNNNNNTAC	2 cut(s)	933, 965
BbsI	GAAGAC	2 cut(s)	494, 1116
Bbv12I	GWGCWC	1 cut(s)	1248
BbvCI	CCTCAGC	1 cut(s)	1776
BbvI	GCAGC	7 cut(s)	1381, 1384, 1755, 2342, 2351, 2626, 2863
BciT130I	CCWGG	2 cut(s)	1115, 2453
BciVI	GTATCC	2 cut(s)	868, 2596
BcnI	CCSGG	1 cut(s)	2998
BcoDI	GTCTC	5 cut(s)	241, 449, 1853, 2503, 2819
BcuI	ACTAGT	1 cut(s)	2555
BfaI	CTAG	9 cut(s)	201, 467, 740, 848, 1740, 2027, 2556, 2664, 3225
BfmI	CTRYAG	2 cut(s)	1841, 2233
BfoI	RGCGCY	1 cut(s)	10
BfrI	CTTAAG	1 cut(s)	2117
BfuI	GTATCC	2 cut(s)	868, 2596
BglII	AGATCT	2 cut(s)	523, 2590
BisI	GCNGC	8 cut(s)	127, 1395, 1398, 1744, 2331, 2340, 2615, 2877
BlpI	GCTNAGC	1 cut(s)	1401
BlsI	GCNGC	8 cut(s)	128, 1396, 1399, 1745, 2332, 2341, 2616, 2878
BmcAI	AGTACT	4 cut(s)	143, 1075, 2435, 3026
Bme1390I	CCNGG	3 cut(s)	1115, 2453, 2998
Bme18I	GGWCC	6 cut(s)	212, 920, 1006, 2100, 3005, 3089
BmeRI	GACNNNNNGTC	1 cut(s)	31
BmgBI	CACGTC	1 cut(s)	2077
BmgT120I	GGNCC	7 cut(s)	212, 699, 920, 1006, 2100, 3005, 3089
BmiI	GGNNCC	9 cut(s)	213, 380, 399, 424, 1153, 2102, 2506, 2995, 3006
BmrFI	CCNGG	3 cut(s)	1115, 2453, 2998
BmrI	ACTGGG	4 cut(s)	34, 1917, 2425, 3265
BmsI	GCATC	9 cut(s)	336, 586, 775, 1175, 1369, 1890, 2313, 2601, 2626
BmtI	GCTAGC	1 cut(s)	1743
BmuI	ACTGGG	4 cut(s)	34, 1917, 2425, 3265
BpiI	GAAGAC	2 cut(s)	494, 1116
BpmI	CTGGAG	4 cut(s)	171, 828, 1062, 1514
Bpu10I	CCTNAGC	3 cut(s)	762, 1776, 1965
Bpu1102I	GCTNAGC	1 cut(s)	1401
BpuEI	CTTGAG	2 cut(s)	1353, 1463
BpuMI	CCSGG	1 cut(s)	2998
Bsa29I	ATCGAT	1 cut(s)	2091
BsaAI	YACGTR	2 cut(s)	1667, 3039
BsaBI	GATNNNNATC	2 cut(s)	600, 2589
BsaI	GGTCTC	1 cut(s)	2503
BsaJI	CCNNGG	3 cut(s)	923, 1114, 2451
BsaWI	WCCGGW	1 cut(s)	2786
BsaXI	ACNNNNNCTCC	4 cut(s)	699, 729, 1060, 1090
Bsc4I	CCNNNNNNNGG	4 cut(s)	832, 917, 2921, 3276
Bse118I	RCCGGY	1 cut(s)	1262
Bse21I	CCTNAGG	1 cut(s)	2070
Bse3DI	GCAATG	3 cut(s)	733, 899, 1219
Bse8I	GATNNNNATC	2 cut(s)	600, 2589
BseAI	TCCGGA	1 cut(s)	2786
BseBI	CCWGG	2 cut(s)	1115, 2453
BseCI	ATCGAT	1 cut(s)	2091
BseDI	CCNNGG	3 cut(s)	923, 1114, 2451
BseJI	GATNNNNATC	2 cut(s)	600, 2589
BseLI	CCNNNNNNNGG	4 cut(s)	832, 917, 2921, 3276
BseMI	GCAATG	3 cut(s)	733, 899, 1219
BseMII	CTCAG	6 cut(s)	294, 753, 1415, 1767, 1956, 2608
BseRI	GAGGAG	2 cut(s)	1256, 2777
BseSI	GKGCMC	1 cut(s)	644
BseXI	GCAGC	7 cut(s)	1381, 1384, 1755, 2342, 2351, 2626, 2863
BshFI	GGCC	2 cut(s)	701, 3046
BshNI	GGYRCC	3 cut(s)	397, 422, 1151
BshVI	ATCGAT	1 cut(s)	2091
BsiHKAI	GWGCWC	1 cut(s)	1248
BsiSI	CCGG	3 cut(s)	1263, 2787, 2997
BslFI	GGGAC	8 cut(s)	549, 1131, 1674, 1925, 2086, 2413, 2546, 3253
BslI	CCNNNNNNNGG	4 cut(s)	832, 917, 2921, 3276
BsmAI	GTCTC	5 cut(s)	241, 449, 1853, 2503, 2819
BsmBI	CGTCTC	1 cut(s)	1853
BsmFI	GGGAC	8 cut(s)	549, 1131, 1674, 1925, 2086, 2413, 2546, 3253
BsmI	GAATGC	1 cut(s)	1231
BsnI	GGCC	2 cut(s)	701, 3046
Bso31I	GGTCTC	1 cut(s)	2503
Bsp1286I	GDGCHC	2 cut(s)	644, 1248
Bsp13I	TCCGGA	1 cut(s)	2786
Bsp1407I	TGTACA	2 cut(s)	717, 1353
Bsp143I	GATC	8 cut(s)	406, 481, 523, 1446, 1834, 2590, 2690, 2898
Bsp1720I	GCTNAGC	1 cut(s)	1401
BspACI	CCGC	6 cut(s)	127, 607, 685, 1637, 2645, 3074
BspANI	GGCC	2 cut(s)	701, 3046
BspCNI	CTCAG	6 cut(s)	293, 754, 1414, 1768, 1957, 2607
BspDI	ATCGAT	1 cut(s)	2091
BspEI	TCCGGA	1 cut(s)	2786
BspHI	TCATGA	2 cut(s)	132, 975
BspLI	GGNNCC	9 cut(s)	213, 380, 399, 424, 1153, 2102, 2506, 2995, 3006
BspMAI	CTGCAG	1 cut(s)	1845
BspOI	GCTAGC	1 cut(s)	1743
BspPI	GGATC	3 cut(s)	1842, 2685, 2906
BspQI	GCTCTTC	2 cut(s)	1010, 1276
BspT107I	GGYRCC	3 cut(s)	397, 422, 1151
BspTI	CTTAAG	1 cut(s)	2117
BspTNI	GGTCTC	1 cut(s)	2503
BsrDI	GCAATG	3 cut(s)	733, 899, 1219
BsrFI	RCCGGY	1 cut(s)	1262
BsrGI	TGTACA	2 cut(s)	717, 1353
BssAI	RCCGGY	1 cut(s)	1262
BssECI	CCNNGG	3 cut(s)	923, 1114, 2451
BssMI	GATC	8 cut(s)	406, 481, 523, 1446, 1834, 2590, 2690, 2898
BssNAI	GTATAC	3 cut(s)	81, 2931, 3036
Bst1107I	GTATAC	3 cut(s)	81, 2931, 3036
Bst2UI	CCWGG	2 cut(s)	1115, 2453
Bst4CI	ACNGT	4 cut(s)	53, 1297, 1318, 2608
Bst6I	CTCTTC	4 cut(s)	954, 1010, 1276, 1695
BstAFI	CTTAAG	1 cut(s)	2117
BstAPI	GCANNNNNTGC	1 cut(s)	239
BstAUI	TGTACA	2 cut(s)	717, 1353
BstBAI	YACGTR	2 cut(s)	1667, 3039
BstC8I	GCNNGC	7 cut(s)	160, 1741, 1841, 1885, 2328, 2805, 3072
BstDEI	CTNAG	7 cut(s)	280, 762, 1401, 1776, 1965, 2070, 2594
BstDSI	CCRYGG	1 cut(s)	923
BstH2I	RGCGCY	1 cut(s)	10
BstHHI	GCGC	2 cut(s)	9, 3248
BstKTI	GATC	8 cut(s)	409, 484, 526, 1449, 1837, 2593, 2693, 2901
BstMAI	GTCTC	5 cut(s)	241, 449, 1853, 2503, 2819
BstMBI	GATC	8 cut(s)	406, 481, 523, 1446, 1834, 2590, 2690, 2898
BstMWI	GCNNNNNNNGC	5 cut(s)	239, 785, 1583, 2336, 2339
BstNI	CCWGG	2 cut(s)	1115, 2453
BstNSI	RCATGY	4 cut(s)	162, 640, 1605, 2735
BstSCI	CCNGG	3 cut(s)	1113, 2451, 2996
BstSFI	CTRYAG	2 cut(s)	1841, 2233
BstSLI	GKGCMC	1 cut(s)	644
BstSNI	TACGTA	2 cut(s)	1667, 3039
BstV1I	GCAGC	7 cut(s)	1381, 1384, 1755, 2342, 2351, 2626, 2863
BstV2I	GAAGAC	2 cut(s)	494, 1116
BstX2I	RGATCY	3 cut(s)	523, 2590, 2898
BstXI	CCANNNNNNTGG	1 cut(s)	2967
BstYI	RGATCY	3 cut(s)	523, 2590, 2898
BstZ17I	GTATAC	3 cut(s)	81, 2931, 3036
Bsu15I	ATCGAT	1 cut(s)	2091
Bsu36I	CCTNAGG	1 cut(s)	2070
BsuI	GTATCC	2 cut(s)	868, 2596
BsuRI	GGCC	2 cut(s)	701, 3046
BsuTUI	ATCGAT	1 cut(s)	2091
BtgI	CCRYGG	1 cut(s)	923
BtrI	CACGTC	1 cut(s)	2077
BtsI	GCAGTG	2 cut(s)	720, 1923
BtsIMutI	CAGTG	9 cut(s)	195, 720, 720, 1323, 1923, 2314, 2581, 2602, 2911
Cac8I	GCNNGC	7 cut(s)	160, 1741, 1841, 1885, 2328, 2805, 3072
CaiI	CAGNNNCTG	1 cut(s)	239
CciI	TCATGA	2 cut(s)	132, 975
CfoI	GCGC	2 cut(s)	9, 3248
Cfr10I	RCCGGY	1 cut(s)	1262
Cfr13I	GGNCC	7 cut(s)	212, 699, 920, 1006, 2100, 3005, 3089
ClaI	ATCGAT	1 cut(s)	2091
CspCI	CAANNNNNGTGG	2 cut(s)	2610, 2645
DdeI	CTNAG	7 cut(s)	280, 762, 1401, 1776, 1965, 2070, 2594
DpnI	GATC	8 cut(s)	408, 483, 525, 1448, 1836, 2592, 2692, 2900
DpnII	GATC	8 cut(s)	406, 481, 523, 1446, 1834, 2590, 2690, 2898
DriI	GACNNNNNGTC	1 cut(s)	31
Eam1104I	CTCTTC	4 cut(s)	954, 1010, 1276, 1695
Eam1105I	GACNNNNNGTC	1 cut(s)	31
EarI	CTCTTC	4 cut(s)	954, 1010, 1276, 1695
EciI	GGCGGA	1 cut(s)	3089
Ecl136II	GAGCTC	1 cut(s)	1246
Eco105I	TACGTA	2 cut(s)	1667, 3039
Eco24I	GRGCYC	1 cut(s)	1248
Eco31I	GGTCTC	1 cut(s)	2503
Eco47I	GGWCC	6 cut(s)	212, 920, 1006, 2100, 3005, 3089
Eco47III	AGCGCT	1 cut(s)	8
Eco53kI	GAGCTC	1 cut(s)	1246
Eco81I	CCTNAGG	1 cut(s)	2070
EcoICRI	GAGCTC	1 cut(s)	1246
EcoRII	CCWGG	2 cut(s)	1113, 2451
EcoT22I	ATGCAT	1 cut(s)	1603
EcoT38I	GRGCYC	1 cut(s)	1248
Esp3I	CGTCTC	1 cut(s)	1853
FalI	AAGNNNNNCTT	6 cut(s)	1368, 1400, 1965, 1997, 2190, 2222
FaqI	GGGAC	8 cut(s)	549, 1131, 1674, 1925, 2086, 2413, 2546, 3253
FauI	CCCGC	1 cut(s)	2638
FauNDI	CATATG	1 cut(s)	1410
FblI	GTMKAC	5 cut(s)	80, 2930, 2953, 3035, 3284
Fnu4HI	GCNGC	8 cut(s)	127, 1395, 1398, 1744, 2331, 2340, 2615, 2877
FriOI	GRGCYC	1 cut(s)	1248
Fsp4HI	GCNGC	8 cut(s)	127, 1395, 1398, 1744, 2331, 2340, 2615, 2877
FspBI	CTAG	9 cut(s)	201, 467, 740, 848, 1740, 2027, 2556, 2664, 3225
GlaI	GCGC	2 cut(s)	8, 3247
GluI	GCNGC	8 cut(s)	127, 1395, 1398, 1744, 2331, 2340, 2615, 2877
GsuI	CTGGAG	4 cut(s)	171, 828, 1062, 1514
HaeII	RGCGCY	1 cut(s)	10
HaeIII	GGCC	2 cut(s)	701, 3046
HapII	CCGG	3 cut(s)	1263, 2787, 2997
HhaI	GCGC	2 cut(s)	9, 3248
Hin6I	GCGC	2 cut(s)	7, 3246
HinP1I	GCGC	2 cut(s)	7, 3246
HincII	GTYRAC	2 cut(s)	804, 1642
HindII	GTYRAC	2 cut(s)	804, 1642
HindIII	AAGCTT	4 cut(s)	770, 1479, 2133, 2805
HinfI	GANTC	6 cut(s)	32, 136, 193, 2215, 2659, 3010
HpaII	CCGG	3 cut(s)	1263, 2787, 2997
HphI	GGTGA	3 cut(s)	850, 1543, 2467
HpyAV	CCTTC	9 cut(s)	173, 283, 865, 911, 1528, 1545, 2312, 2890, 3222
HpyCH4III	ACNGT	4 cut(s)	53, 1297, 1318, 2608
HpyCH4IV	ACGT	3 cut(s)	1666, 2076, 3038
HpyF10VI	GCNNNNNNNGC	5 cut(s)	239, 785, 1583, 2336, 2339
HpyF3I	CTNAG	7 cut(s)	280, 762, 1401, 1776, 1965, 2070, 2594
HpySE526I	ACGT	3 cut(s)	1666, 2076, 3038
HspAI	GCGC	2 cut(s)	7, 3246
Kpn2I	TCCGGA	1 cut(s)	2786
KpnI	GGTACC	2 cut(s)	426, 1155
Kzo9I	GATC	8 cut(s)	406, 481, 523, 1446, 1834, 2590, 2690, 2898
LguI	GCTCTTC	2 cut(s)	1010, 1276
LmnI	GCTCC	5 cut(s)	384, 809, 1243, 2993, 3108
Lsp1109I	GCAGC	7 cut(s)	1381, 1384, 1755, 2342, 2351, 2626, 2863
LweI	GCATC	9 cut(s)	336, 586, 775, 1175, 1369, 1890, 2313, 2601, 2626
MaeI	CTAG	9 cut(s)	201, 467, 740, 848, 1740, 2027, 2556, 2664, 3225
MaeII	ACGT	3 cut(s)	1666, 2076, 3038
MaeIII	GTNAC	8 cut(s)	53, 174, 365, 677, 1819, 2182, 2228, 2399
MalI	GATC	8 cut(s)	408, 483, 525, 1448, 1836, 2592, 2692, 2900
MboI	GATC	8 cut(s)	406, 481, 523, 1446, 1834, 2590, 2690, 2898
MfeI	CAATTG	1 cut(s)	1655
MflI	RGATCY	3 cut(s)	523, 2590, 2898
MhlI	GDGCHC	2 cut(s)	644, 1248
MlyI	GAGTC	1 cut(s)	41
MmeI	TCCRAC	1 cut(s)	2971
Mph1103I	ATGCAT	1 cut(s)	1603
MroI	TCCGGA	1 cut(s)	2786
MroXI	GAANNNNTTC	2 cut(s)	1587, 3026
MslI	CAYNNNNRTG	1 cut(s)	2736
MspA1I	CMGCKG	3 cut(s)	169, 779, 2342
MspCI	CTTAAG	1 cut(s)	2117
MspI	CCGG	3 cut(s)	1263, 2787, 2997
MspR9I	CCNGG	3 cut(s)	1115, 2453, 2998
MunI	CAATTG	1 cut(s)	1655
Mva1269I	GAATGC	1 cut(s)	1231
MvaI	CCWGG	2 cut(s)	1115, 2453
MwoI	GCNNNNNNNGC	5 cut(s)	239, 785, 1583, 2336, 2339
NciI	CCSGG	1 cut(s)	2998
NdeI	CATATG	1 cut(s)	1410
NdeII	GATC	8 cut(s)	406, 481, 523, 1446, 1834, 2590, 2690, 2898
NheI	GCTAGC	1 cut(s)	1739
NlaIV	GGNNCC	9 cut(s)	213, 380, 399, 424, 1153, 2102, 2506, 2995, 3006
NmuCI	GTSAC	4 cut(s)	53, 174, 2182, 2228
NsiI	ATGCAT	1 cut(s)	1603
NspI	RCATGY	4 cut(s)	162, 640, 1605, 2735
PaeI	GCATGC	1 cut(s)	162
PagI	TCATGA	2 cut(s)	132, 975
PciI	ACATGT	1 cut(s)	636
PciSI	GCTCTTC	2 cut(s)	1010, 1276
PctI	GAATGC	1 cut(s)	1231
PdmI	GAANNNNTTC	2 cut(s)	1587, 3026
PfeI	GAWTC	5 cut(s)	136, 193, 2215, 2659, 3010
PflMI	CCANNNNNTGG	1 cut(s)	832
PkrI	GCNGC	8 cut(s)	128, 1396, 1399, 1745, 2332, 2341, 2616, 2878
PleI	GAGTC	1 cut(s)	40
PpsI	GAGTC	1 cut(s)	40
Ppu21I	YACGTR	2 cut(s)	1667, 3039
PscI	ACATGT	1 cut(s)	636
PsiI	TTATAA	2 cut(s)	2445, 2541
Psp124BI	GAGCTC	1 cut(s)	1248
Psp6I	CCWGG	2 cut(s)	1113, 2451
PspGI	CCWGG	2 cut(s)	1113, 2451
PspN4I	GGNNCC	9 cut(s)	213, 380, 399, 424, 1153, 2102, 2506, 2995, 3006
PspPI	GGNCC	7 cut(s)	212, 699, 920, 1006, 2100, 3005, 3089
PstI	CTGCAG	1 cut(s)	1845
PstNI	CAGNNNCTG	1 cut(s)	239
PsuI	RGATCY	3 cut(s)	523, 2590, 2898
PvuII	CAGCTG	3 cut(s)	169, 779, 2342
RseI	CAYNNNNRTG	1 cut(s)	2736
SacI	GAGCTC	1 cut(s)	1248
SapI	GCTCTTC	2 cut(s)	1010, 1276
SatI	GCNGC	8 cut(s)	127, 1395, 1398, 1744, 2331, 2340, 2615, 2877
Sau3AI	GATC	8 cut(s)	406, 481, 523, 1446, 1834, 2590, 2690, 2898
Sau96I	GGNCC	7 cut(s)	212, 699, 920, 1006, 2100, 3005, 3089
SbfI	CCTGCAGG	1 cut(s)	1845
ScaI	AGTACT	4 cut(s)	143, 1075, 2435, 3026
SchI	GAGTC	1 cut(s)	41
ScrFI	CCNGG	3 cut(s)	1115, 2453, 2998
SdaI	CCTGCAGG	1 cut(s)	1845
SduI	GDGCHC	2 cut(s)	644, 1248
SfaNI	GCATC	9 cut(s)	336, 586, 775, 1175, 1369, 1890, 2313, 2601, 2626
SfcI	CTRYAG	2 cut(s)	1841, 2233
SinI	GGWCC	6 cut(s)	212, 920, 1006, 2100, 3005, 3089
SmiMI	CAYNNNNRTG	1 cut(s)	2736
SmlI	CTYRAG	3 cut(s)	1368, 1442, 2117
SmoI	CTYRAG	3 cut(s)	1368, 1442, 2117
SnaBI	TACGTA	2 cut(s)	1667, 3039
SpeI	ACTAGT	1 cut(s)	2555
SphI	GCATGC	1 cut(s)	162
Sse8387I	CCTGCAGG	1 cut(s)	1845
SsiI	CCGC	6 cut(s)	127, 607, 685, 1637, 2645, 3074
SspI	AATATT	3 cut(s)	1438, 2154, 2979
SspMI	CTAG	9 cut(s)	201, 467, 740, 848, 1740, 2027, 2556, 2664, 3225
SstI	GAGCTC	1 cut(s)	1248
StyD4I	CCNGG	3 cut(s)	1113, 2451, 2996
TaaI	ACNGT	4 cut(s)	53, 1297, 1318, 2608
TaiI	ACGT	3 cut(s)	1669, 2079, 3041
TaqI	TCGA	5 cut(s)	952, 2091, 3255, 3275, 3290
TatI	WGTACW	8 cut(s)	141, 203, 717, 1073, 1128, 1353, 2433, 3024
TauI	GCSGC	1 cut(s)	129
TfiI	GAWTC	5 cut(s)	136, 193, 2215, 2659, 3010
TscAI	CASTG	9 cut(s)	195, 720, 727, 1323, 1930, 2314, 2581, 2602, 2911
TseFI	GTSAC	4 cut(s)	53, 174, 2182, 2228
TseI	GCWGC	7 cut(s)	1394, 1397, 1743, 2330, 2339, 2614, 2876
Tsp45I	GTSAC	4 cut(s)	53, 174, 2182, 2228
TspGWI	ACGGA	1 cut(s)	912
TspRI	CASTG	9 cut(s)	195, 720, 727, 1323, 1930, 2314, 2581, 2602, 2911
Van91I	CCANNNNNTGG	1 cut(s)	832
Vha464I	CTTAAG	1 cut(s)	2117
VpaK11BI	GGWCC	6 cut(s)	212, 920, 1006, 2100, 3005, 3089
XbaI	TCTAGA	1 cut(s)	2026
XceI	RCATGY	4 cut(s)	162, 640, 1605, 2735
XcmI	CCANNNNNNNNNTGG	1 cut(s)	108
XmiI	GTMKAC	5 cut(s)	80, 2930, 2953, 3035, 3284
XmnI	GAANNNNTTC	2 cut(s)	1587, 3026
XspI	CTAG	9 cut(s)	201, 467, 740, 848, 1740, 2027, 2556, 2664, 3225
ZrmI	AGTACT	4 cut(s)	143, 1075, 2435, 3026
Zsp2I	ATGCAT	1 cut(s)	1603

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.