RLG00000002345

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006243
Physical Location & Seq
Reverse (-)
32553626 .. 32558660
5035 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002345

Sequence Viewer

Length: 1242 bp
ATGCTATATGCCTATATAGAGACTGCAGCCCTAAATGGAGAACCTGATCTGAAGACCAGGGTCATACCATCAGCTTGTATGGGGATAGATCTTGAACTGTTGCACAAAATTAAGGGTGTAATTGAGTGCCCTAATCGTGATAAGGATATTCGAAGATTTGATGCAAATATGTGGTTGTTTCCCCCATTTATTGATAATGACCTCTGCCCATTAACCATAAAGAATACTATTCTTCAGTCATGTTACTTGCGAAATACAGAGTGGGCCTGTGGAGTAGCAGTCTACACTGGCAATGTAACCAAGCTTGGTATGACTAGGGGCATTCCTGAGCCAAAGCTTACAGCTGTTGATGCTATGATTGGCAAGTTGACAGGAGCTATATTTGTTTTCCAAGTAGTGGTGGTTATGGTTCTAGGCGTTGCTGGTAATGTCTGGAAGGATACAGAAGCAAGCAAGGTATCTCTCGACCTTGTCAAGAGCTTGTACGCAAAGTTTATTGATTGGGATAGTAAAATGATTGACCGAGAAATGGCAACTCCAGCCCATGCAACAAAAATAAGTGAAGATCTGGGACAAGTTGAGTACATTCTGACTGATAAAACTGGTACCCTAATGGAAAATAAAATGATATTTAGAAGGTGTTGTATCAGAGGCATTTACTATGGGAATGAGAATGGAAATGCCTTAGAAGATAAGGAGCTCATTGATGCTATTTCCAGTGGCTCTTCTGATGTTATACATTTCCTTACAGTTATGGCGATATGTAATACAGTCATACCCATTCAAAGCAAAAATGGAAGTATCGTGTACAATCTCAAGATGAGGATGCTCTTGTTCATGCTGCTGCTCAGCTACGTATGGCTTTTGTCAATAAAAATGCTAACATTCTTGATTGCAGAGATCAAATTCAACGGTTCTACAGTTAAGTATGAAGCCTTGGATATTCTGGAGTTAACTTCTGATGGAAAAAGAATGTCAGTAGTGGTAAAGGATTGTCACAATGGAAGGATTATTCTTTTATCAAAGGGAGCAGATGAATCAGTTCTTCCTTATGCATGTGCTGGACAGCAAACAAGGAATATTATTGAAGCTGTTGAGCAATATGCTCAGTTGGGACTACGGACACTGTGTCTAGCTTGGCGTGAACTAAAAGAAGAGGAATATCGAGAATGGTCTTTGGTGTATAAAGAGGCTAGCAGCACATTGGTTGATAGGGAGATACAGACATGTGATCTAATATAA

Protein Analysis

414

Amino Acids

46.44

Weight (kDa)

5.5

Isoelectric Point (pI)

39.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase PF13246 302 - 389 2.6e-08 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 605
AccB1I GGYRCC 1 cut(s) 605
AccB7I CCANNNNNTGG 1 cut(s) 397
AccI GTMKAC 1 cut(s) 282
AcsI RAATTY 1 cut(s) 905
AcuI CTGAAG 2 cut(s) 71, 218
AfaI GTAC 4 cut(s) 485, 584, 607, 809
AfiI CCNNNNNNNGG 2 cut(s) 397, 529
AflIII ACRYGT 1 cut(s) 1226
AgsI TTSAA 4 cut(s) 95, 785, 910, 1088
AhdI GACNNNNNGTC 1 cut(s) 1128
AjnI CCWGG 1 cut(s) 56
Alw21I GWGCWC 1 cut(s) 702
Alw26I GTCTC 1 cut(s) 14
AoxI GGCC 1 cut(s) 264
ApeKI GCWGC 4 cut(s) 26, 841, 844, 1197
ApoI RAATTY 1 cut(s) 905
Asp700I GAANNNNTTC 1 cut(s) 1041
Asp718I GGTACC 1 cut(s) 605
AspS9I GGNCC 1 cut(s) 264
AsuII TTCGAA 1 cut(s) 151
AsuNHI GCTAGC 1 cut(s) 1193
BaeGI GKGCMC 1 cut(s) 131
BanI GGYRCC 1 cut(s) 605
BanII GRGCYC 1 cut(s) 702
BbsI GAAGAC 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 702
BbvI GCAGC 4 cut(s) 38, 828, 831, 1209
BccI CCATC 2 cut(s) 76, 956
BciT130I CCWGG 1 cut(s) 58
BciVI GTATCC 1 cut(s) 433
BcoDI GTCTC 1 cut(s) 14
BfaI CTAG 4 cut(s) 315, 413, 1133, 1194
BfmI CTRYAG 2 cut(s) 24, 918
BfuI GTATCC 1 cut(s) 433
BglII AGATCT 2 cut(s) 88, 565
BisI GCNGC 4 cut(s) 27, 842, 845, 1198
BlpI GCTNAGC 1 cut(s) 848
BlsI GCNGC 4 cut(s) 28, 843, 846, 1199
Bme1390I CCNGG 1 cut(s) 58
BmeRI GACNNNNNGTC 1 cut(s) 1128
BmgT120I GGNCC 1 cut(s) 264
BmiI GGNNCC 1 cut(s) 607
BmrFI CCNGG 1 cut(s) 58
BmsI GCATC 4 cut(s) 151, 340, 697, 816
BmtI GCTAGC 1 cut(s) 1197
BoxI GACNNNNGTC 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 59
BpmI CTGGAG 2 cut(s) 522, 968
Bpu10I CCTNAGC 1 cut(s) 327
Bpu1102I GCTNAGC 1 cut(s) 848
Bpu14I TTCGAA 1 cut(s) 151
BpuEI CTTGAG 1 cut(s) 800
BsaAI YACGTR 1 cut(s) 856
BsaJI CCNNGG 2 cut(s) 57, 936
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 2 cut(s) 397, 529
Bse1I ACTGG 3 cut(s) 292, 607, 717
Bse3DI GCAATG 1 cut(s) 298
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 2 cut(s) 57, 936
BseGI GGATG 1 cut(s) 831
BseLI CCNNNNNNNGG 2 cut(s) 397, 529
BseMI GCAATG 1 cut(s) 298
BseMII CTCAG 3 cut(s) 318, 862, 1121
BseNI ACTGG 3 cut(s) 292, 607, 717
BseSI GKGCMC 1 cut(s) 131
BseXI GCAGC 4 cut(s) 38, 828, 831, 1209
BshFI GGCC 1 cut(s) 266
BshNI GGYRCC 1 cut(s) 605
BsiHKAI GWGCWC 1 cut(s) 702
BslFI GGGAC 2 cut(s) 585, 1128
BslI CCNNNNNNNGG 2 cut(s) 397, 529
BsmAI GTCTC 1 cut(s) 14
BsmFI GGGAC 2 cut(s) 585, 1128
BsmI GAATGC 1 cut(s) 321
BsnI GGCC 1 cut(s) 266
Bsp119I TTCGAA 1 cut(s) 151
Bsp1286I GDGCHC 2 cut(s) 131, 702
Bsp1407I TGTACA 1 cut(s) 807
Bsp143I GATC 5 cut(s) 46, 88, 565, 900, 1231
Bsp1720I GCTNAGC 1 cut(s) 848
BspANI GGCC 1 cut(s) 266
BspCNI CTCAG 3 cut(s) 319, 861, 1120
BspLI GGNNCC 1 cut(s) 607
BspMAI CTGCAG 1 cut(s) 28
BspOI GCTAGC 1 cut(s) 1197
BspQI GCTCTTC 1 cut(s) 730
BspT104I TTCGAA 1 cut(s) 151
BspT107I GGYRCC 1 cut(s) 605
BsrDI GCAATG 1 cut(s) 298
BsrGI TGTACA 1 cut(s) 807
BsrI ACTGG 3 cut(s) 292, 607, 717
BssECI CCNNGG 2 cut(s) 57, 936
BssMI GATC 5 cut(s) 46, 88, 565, 900, 1231
BssT1I CCWWGG 1 cut(s) 936
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 6 cut(s) 99, 751, 772, 914, 922, 1128
Bst6I CTCTTC 2 cut(s) 730, 1149
BstAUI TGTACA 1 cut(s) 807
BstBAI YACGTR 1 cut(s) 856
BstBI TTCGAA 1 cut(s) 151
BstC8I GCNNGC 2 cut(s) 451, 1195
BstDEI CTNAG 4 cut(s) 327, 685, 848, 1107
BstF5I GGATG 1 cut(s) 831
BstKTI GATC 5 cut(s) 49, 91, 568, 903, 1234
BstMAI GTCTC 1 cut(s) 14
BstMBI GATC 5 cut(s) 46, 88, 565, 900, 1231
BstMWI GCNNNNNNNGC 2 cut(s) 350, 539
BstNI CCWGG 1 cut(s) 58
BstNSI RCATGY 2 cut(s) 1059, 1230
BstPAI GACNNNNGTC 1 cut(s) 59
BstSCI CCNGG 1 cut(s) 56
BstSFI CTRYAG 2 cut(s) 24, 918
BstSLI GKGCMC 1 cut(s) 131
BstSNI TACGTA 1 cut(s) 856
BstV1I GCAGC 4 cut(s) 38, 828, 831, 1209
BstV2I GAAGAC 1 cut(s) 59
BstX2I RGATCY 2 cut(s) 88, 565
BstYI RGATCY 2 cut(s) 88, 565
BsuI GTATCC 1 cut(s) 433
BsuRI GGCC 1 cut(s) 266
BtsCI GGATG 1 cut(s) 831
BtsIMutI CAGTG 3 cut(s) 285, 724, 1124
Cac8I GCNNGC 2 cut(s) 451, 1195
Cfr13I GGNCC 1 cut(s) 264
Csp6I GTAC 4 cut(s) 484, 583, 606, 808
CviAII CATG 5 cut(s) 240, 545, 838, 1056, 1227
CviQI GTAC 4 cut(s) 484, 583, 606, 808
DdeI CTNAG 4 cut(s) 327, 685, 848, 1107
DpnI GATC 5 cut(s) 48, 90, 567, 902, 1233
DpnII GATC 5 cut(s) 46, 88, 565, 900, 1231
DriI GACNNNNNGTC 1 cut(s) 1128
Eam1104I CTCTTC 2 cut(s) 730, 1149
Eam1105I GACNNNNNGTC 1 cut(s) 1128
EarI CTCTTC 2 cut(s) 730, 1149
Ecl136II GAGCTC 1 cut(s) 700
Eco105I TACGTA 1 cut(s) 856
Eco130I CCWWGG 1 cut(s) 936
Eco24I GRGCYC 1 cut(s) 702
Eco53kI GAGCTC 1 cut(s) 700
Eco57I CTGAAG 2 cut(s) 71, 218
EcoICRI GAGCTC 1 cut(s) 700
EcoRII CCWGG 1 cut(s) 56
EcoT14I CCWWGG 1 cut(s) 936
EcoT22I ATGCAT 1 cut(s) 1057
EcoT38I GRGCYC 1 cut(s) 702
ErhI CCWWGG 1 cut(s) 936
FaeI CATG 5 cut(s) 243, 548, 841, 1059, 1230
FaqI GGGAC 2 cut(s) 585, 1128
FatI CATG 5 cut(s) 239, 544, 837, 1055, 1226
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 4 cut(s) 27, 842, 845, 1198
FokI GGATG 1 cut(s) 838
FriOI GRGCYC 1 cut(s) 702
Fsp4HI GCNGC 4 cut(s) 27, 842, 845, 1198
FspBI CTAG 4 cut(s) 315, 413, 1133, 1194
GluI GCNGC 4 cut(s) 27, 842, 845, 1198
GsuI CTGGAG 2 cut(s) 522, 968
HaeIII GGCC 1 cut(s) 266
Hin1II CATG 5 cut(s) 243, 548, 841, 1059, 1230
HincII GTYRAC 2 cut(s) 369, 954
HindII GTYRAC 2 cut(s) 369, 954
HindIII AAGCTT 2 cut(s) 302, 335
HinfI GANTC 1 cut(s) 1037
HpaI GTTAAC 1 cut(s) 954
Hpy166II GTNNAC 5 cut(s) 283, 369, 808, 954, 1145
Hpy188I TCNGA 5 cut(s) 51, 591, 650, 730, 961
Hpy8I GTNNAC 5 cut(s) 283, 369, 808, 954, 1145
HpyAV CCTTC 3 cut(s) 430, 630, 999
HpyCH4III ACNGT 6 cut(s) 99, 751, 772, 914, 922, 1128
HpyCH4IV ACGT 1 cut(s) 855
HpyCH4V TGCA 6 cut(s) 26, 103, 164, 548, 896, 1055
HpyF10VI GCNNNNNNNGC 2 cut(s) 350, 539
HpyF3I CTNAG 4 cut(s) 327, 685, 848, 1107
HpySE526I ACGT 1 cut(s) 855
Hsp92II CATG 5 cut(s) 243, 548, 841, 1059, 1230
KpnI GGTACC 1 cut(s) 609
KspAI GTTAAC 1 cut(s) 954
Kzo9I GATC 5 cut(s) 46, 88, 565, 900, 1231
LguI GCTCTTC 1 cut(s) 730
LmnI GCTCC 3 cut(s) 374, 697, 1028
Lsp1109I GCAGC 4 cut(s) 38, 828, 831, 1209
LweI GCATC 4 cut(s) 151, 340, 697, 816
MaeI CTAG 4 cut(s) 315, 413, 1133, 1194
MaeII ACGT 1 cut(s) 855
MaeIII GTNAC 3 cut(s) 242, 295, 995
MalI GATC 5 cut(s) 48, 90, 567, 902, 1233
MboI GATC 5 cut(s) 46, 88, 565, 900, 1231
MboII GAAGA 8 cut(s) 64, 165, 224, 575, 701, 717, 1037, 1166
MflI RGATCY 2 cut(s) 88, 565
MhlI GDGCHC 2 cut(s) 131, 702
MluCI AATT 3 cut(s) 108, 120, 905
MnlI CCTC 5 cut(s) 212, 644, 816, 1150, 1183
Mph1103I ATGCAT 1 cut(s) 1057
MroXI GAANNNNTTC 1 cut(s) 1041
MseI TTAA 4 cut(s) 111, 212, 924, 953
MspA1I CMGCKG 1 cut(s) 344
MspR9I CCNGG 1 cut(s) 58
Mva1269I GAATGC 1 cut(s) 321
MvaI CCWGG 1 cut(s) 58
MwoI GCNNNNNNNGC 2 cut(s) 350, 539
NdeII GATC 5 cut(s) 46, 88, 565, 900, 1231
NheI GCTAGC 1 cut(s) 1193
NlaIII CATG 5 cut(s) 243, 548, 841, 1059, 1230
NlaIV GGNNCC 1 cut(s) 607
NmuCI GTSAC 1 cut(s) 995
NsiI ATGCAT 1 cut(s) 1057
NspI RCATGY 2 cut(s) 1059, 1230
NspV TTCGAA 1 cut(s) 151
PciI ACATGT 1 cut(s) 1226
PciSI GCTCTTC 1 cut(s) 730
PctI GAATGC 1 cut(s) 321
PdmI GAANNNNTTC 1 cut(s) 1041
PfeI GAWTC 1 cut(s) 1037
PflFI GACNNNGTC 1 cut(s) 470
PflMI CCANNNNNTGG 1 cut(s) 397
PkrI GCNGC 4 cut(s) 28, 843, 846, 1199
Ppu21I YACGTR 1 cut(s) 856
PscI ACATGT 1 cut(s) 1226
PshAI GACNNNNGTC 1 cut(s) 59
Psp124BI GAGCTC 1 cut(s) 702
Psp6I CCWGG 1 cut(s) 56
PspGI CCWGG 1 cut(s) 56
PspN4I GGNNCC 1 cut(s) 607
PspPI GGNCC 1 cut(s) 264
PstI CTGCAG 1 cut(s) 28
PsuI RGATCY 2 cut(s) 88, 565
PsyI GACNNNGTC 1 cut(s) 470
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 4 cut(s) 485, 584, 607, 809
RsaNI GTAC 4 cut(s) 484, 583, 606, 808
SacI GAGCTC 1 cut(s) 702
SapI GCTCTTC 1 cut(s) 730
SaqAI TTAA 4 cut(s) 111, 212, 924, 953
SatI GCNGC 4 cut(s) 27, 842, 845, 1198
Sau3AI GATC 5 cut(s) 46, 88, 565, 900, 1231
Sau96I GGNCC 1 cut(s) 264
ScrFI CCNGG 1 cut(s) 58
SduI GDGCHC 2 cut(s) 131, 702
SfaNI GCATC 4 cut(s) 151, 340, 697, 816
SfcI CTRYAG 2 cut(s) 24, 918
SfuI TTCGAA 1 cut(s) 151
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
SnaBI TACGTA 1 cut(s) 856
Sse9I AATT 3 cut(s) 108, 120, 905
SspI AATATT 1 cut(s) 1081
SspMI CTAG 4 cut(s) 315, 413, 1133, 1194
SstI GAGCTC 1 cut(s) 702
StyD4I CCNGG 1 cut(s) 56
StyI CCWWGG 1 cut(s) 936
TaaI ACNGT 6 cut(s) 99, 751, 772, 914, 922, 1128
TaiI ACGT 1 cut(s) 858
TaqI TCGA 3 cut(s) 151, 465, 1165
TaqII GACCGA 1 cut(s) 537
TasI AATT 3 cut(s) 108, 120, 905
TatI WGTACW 2 cut(s) 582, 807
TfiI GAWTC 1 cut(s) 1037
Tru1I TTAA 4 cut(s) 111, 212, 924, 953
Tru9I TTAA 4 cut(s) 111, 212, 924, 953
TscAI CASTG 3 cut(s) 292, 724, 1131
TseFI GTSAC 1 cut(s) 995
TseI GCWGC 4 cut(s) 26, 841, 844, 1197
Tsp45I GTSAC 1 cut(s) 995
TspDTI ATGAA 3 cut(s) 826, 945, 1050
TspGWI ACGGA 1 cut(s) 1135
TspRI CASTG 3 cut(s) 292, 724, 1131
Tth111I GACNNNGTC 1 cut(s) 470
Van91I CCANNNNNTGG 1 cut(s) 397
XapI RAATTY 1 cut(s) 905
XceI RCATGY 2 cut(s) 1059, 1230
XmiI GTMKAC 1 cut(s) 282
XmnI GAANNNNTTC 1 cut(s) 1041
XspI CTAG 4 cut(s) 315, 413, 1133, 1194
Zsp2I ATGCAT 1 cut(s) 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.