MD17G1235400.v1.1

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006243
Physical Location & Seq
Forward (+)
28430415 .. 28453104
22690 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1235400.v1.1.491

Sequence Viewer

Length: 3321 bp
ATGAAGCGCTACATCTACATAAACGATGACGAGTCATCGCAGTATCTTTACTGTGACAATCGCATATCAAATAGAAAGTACACTCTGTTGAACTTTCTCCCAAAGAATTTATGGGAACAATTCAGCCGCTTCATGAATCAGTACTTTCTGCTGATTGCTTGCCTTCAGCTATGGTCACTTATTACTCCAGTAAATCCTGCTAGTACATGGGGTCCACTTATCTTCATTTTTGCAGTCTCTGCTTCAAAAGAGGCATGGGATGATTACAACCGATATCTCTCAGACAAGAAGGCGAATGAGAAAGAAGTTTGGGTTGTTAGGCAGGGCATCAAGAAACATATTAAAGCACAAGATATTCATGTTGGTAACATCGTTTGGCTCCGAGAGAATGATGAGGTGCCATGTGATCTTGTTTTGATTGGCACCTCTGAAGCACAAGGCCTGTGCTATGTAGAGACTTCTGCCCTAGATGGCGAGACTGATCTGAAGACGAGGGTTATACCCCCAGCTTGCATGGGGATAGATCTTGAATTGTTGCACAAAATTAAGGGTTTAATTGAGTGCCCTAATCCTGATAAGGATATTAGAAGATTTGATGCAAATCTGCGGTTGTTTCCTCCATTTATTGATAATGACTTGTGCCCATTAACAATAAAAAACACACTACTTCAGTCTTGTTACTTGCGGAATACAGAGTGGGCCTGTGGAGTAGCAGTGTACACTGGCAATGAAACCAAGCTAGGTATGAGTAGGGGCATACCCGAGCCGAAGCTTACAGCTGTTGATGCCATGATTGACAAGTTGACTGGAGCTATATTTGTTTTCCAAATAGTGGTGGTGATGGTTCTAGGCGTTGCTGGTAATGTATGGAAGGATACAGAAGCAAGGAAGCAATGGTATGTTCTATATCCGGAGGAAGGTCCGTGGTACGAACTATTAGTAATCCCTCTTCGATTTGAACTACTTTGTTCCATCATGATACCCATATCAATTAAGGTATCTCTCGACCTTGTGAAGGGCTTGTATGCGAAGTTTATTGATTGGGATAATGAAATGATTGACAGAGAAACGAGTACTCCAGCCCATGCAACAAACACAGCAATAAGTGAAGACCTGGGACAAGTAGAGTACATTTTGACTGACAAAACCGGTACCTTAACCGAAAATAGAATGATATTTAGAAGATGCTGTATCAATGGAATTTTCTATGGCAATGAAAATGGGAATGCCTTGATAGATGAGGAGCTCATAAATGCTGTCGCCGGTGGCTCTTCTGATGTTATACGTTTCCTTACAGTTATGGCAATATGTAATACAGTGGTACCTATTCAGAGCAAAAGTGGAAGTATCTTGTACAAGGCACAATCTCAAGATGAGGATGCTCTTGTTCATGCTGCTGCTCAGCTGCATATGGTTTTTGTCAATAAAAATTCAAATACTCTTGAGATCAAGTTTAATGCTTCGACAATTCAATATGAAGCCTTGGAAATTCTGGAGTTCACTTCTGATAGGAAAAGAATGTCAATAGTGGTGAAGGATTGCCAAAATGGCGGGATTATCCTTTTGTCAAAAGGAGCAGATGAAGCAATTCTCCCTTTGGCATGTGCTGGACAGCAAACAAGGACATTTGTTGAAGCTGTTGACCAATATGCTCAATTAGGACTACGTACTCTATGTCTAGCTTGGCGTGAACTAAAAGAAGAAGAATATCAAGAATGGTCTTTGATGTTCAAAGAGGCTAGCAGCACATTAGTTGACAGGGAGTGGAGAATTGCTGAGGTCTGTCAAAGATTAGAGCATGACTTTGAAATTCTTGGAGTTACTGCAATAGAGGATCGCCTACAGGATGGTGTACCAGAGACAATTGAGACTTTGAGAAAAGCTGGCATAAATTTTTGGATGTTGACTGGAGACAAGCAGAATACTGCGATACAGATTGCGCTTTCATGCAATTTCATTTCCCCTGAGCCGAAAGGTCAGCTTTTGTTAATTGATGGCAAAACTGAAGATGAAGTGCGTAGAAGTTTAGAGAGAGTTTTACTTACAATGAGGATAACGACTTCAGAACCTAAGGATGTGGCATTTGCCATCGATGGCTGGTCTCTTGAAATTGCACTCAAGCATTATCGGAAAGCTTTTACTGAATTAGCAATACTGTCAAGGACTGCTATATGTTGTCGTGTGACGCCATCACAAAAAGCTCAGCTTGTGGAGATTTTAAAATCATGTGACTATAGAACATTGGCTATTGGGGATGGTGGAAATGATGTGAGGATGATACAACAAGCAGACATTGGGGTTGGCATCAGTGGTAGAGAAGGACTGCAAGCAGCCAGAGCAGCTGATTATAGTATTGGGAGGTTTCGGTTTCTGAAAAGATTAATACTTGTGCATGGGCGTTACTCGTACAATCGTACAACATTTTTGTCCCAGTACTCCTTTTATAAATCCCTGGTGATATGTTTCATCCAAATTTTTTTCTCTTTTGTTTCAGGTGTTTCTGGAACCAGTCTTTTCAATTCTGTTAGCTTGATGGCTTATAATGTTTTCTACACCAGTATTCCTGTTCTAGTCAGTGTTCTTGACAAAGATCTCAGTGAGGATACTGTGATGCAGCATCCACAAATTTTATTTTACTGCCAAGCGGGGAGGCTTCTGAACCCTAGCACATTTGCTGGATGGTTTGGGCGATCCCTTTTCCATGCAATTATTGTGTTTGTGATTAGCATACATGCCTATGCCTATGAAAGAAGTGAAATGGAGGAGATTTCAATGGTAGCTCTCTCCGGATGTATTTGGTTGCAAGCTTTTGTCATGGCATTAGAGACAAATTCCTTTACACTATTGCAACATCTTGCTATATGGGGCAATTTAGCTGCCTTCTATATCATCAACTGGATCTTCAGTGCCATTCCAACATCGGGAATGTATACAATAATGTTTCGCTTGTGTAGACAGCCATCTTATTGGATGACAATATTGCTCATAGTTGCAGCAGGGATGGGTCCGATTCTAGCTCTGAAGTACTTCAGGTATACGTACAGGCCAAGCAAAATCAATATGCTACAGCAGGCAGAACGATTGGGTGGTCCTATCCTGTCTCTTGGGAGCATTGAGCCACAACCCAGAACCATAGAGAAAGATGTTCCTCCGTTATCGATAACTCAACCCAAGAACAGAAATCCAATTTTCGAACCCCTACTATCAGATACACCAAATTCAACTAGAAGGTCTTTCGGCTCAGGTGCACCTTTCGATTTCTTTCAGTCGCAGTCCAGATTGTCAACGTCTAATTACACAAGAAATTGTAAGGACAACTGA

Protein Analysis

1107

Amino Acids

125.21

Weight (kDa)

5.77

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhoLip_ATPase_N PF16209 5 - 70 5.1e-24 Phospholipid-translocating ATPase N-terminal
E1-E2_ATPase PF00122 99 - 162 3.7e-07 P-type ATPase actuator domain
Cation_ATPase PF13246 427 - 615 1.1e-15 P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C PF16212 778 - 1017 7.2e-66 Phospholipid-translocating P-type ATPase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2445, 2541
Acc65I GGTACC 2 cut(s) 1151, 1321
AccB1I GGYRCC 4 cut(s) 397, 422, 1151, 1321
AccB7I CCANNNNNTGG 1 cut(s) 832
AccI GTMKAC 3 cut(s) 2930, 2953, 3035
AccIII TCCGGA 2 cut(s) 910, 2786
AciI CCGC 5 cut(s) 127, 607, 685, 1551, 2645
AclWI GGATC 3 cut(s) 1842, 2685, 2906
AcuI CTGAAG 9 cut(s) 149, 450, 506, 653, 2025, 2046, 2887, 3013, 3041
AcyI GRCGYC 1 cut(s) 2186
AfeI AGCGCT 1 cut(s) 8
AfiI CCNNNNNNNGG 4 cut(s) 832, 917, 1015, 2921
AgeI ACCGGT 1 cut(s) 1148
AjnI CCWGG 2 cut(s) 1113, 2451
Alw21I GWGCWC 2 cut(s) 1248, 3250
Alw26I GTCTC 9 cut(s) 241, 449, 470, 1853, 1862, 1905, 2106, 2819, 3105
Alw44I GTGCAC 1 cut(s) 3246
AlwI GGATC 3 cut(s) 1842, 2685, 2906
AlwNI CAGNNNCTG 1 cut(s) 239
Ama87I CYCGRG 1 cut(s) 761
Aor13HI TCCGGA 2 cut(s) 910, 2786
Aor51HI AGCGCT 1 cut(s) 8
AoxI GGCC 3 cut(s) 439, 699, 3044
ApaLI GTGCAC 1 cut(s) 3246
ApeKI GCWGC 9 cut(s) 1394, 1397, 1405, 1743, 2330, 2339, 2614, 2876, 2993
AseI ATTAAT 1 cut(s) 2381
AsiGI ACCGGT 1 cut(s) 1148
Asp700I GAANNNNTTC 2 cut(s) 1587, 3026
Asp718I GGTACC 2 cut(s) 1151, 1321
AspLEI GCGC 2 cut(s) 9, 1942
AspS9I GGNCC 5 cut(s) 212, 699, 920, 3005, 3089
AsuHPI GGTGA 3 cut(s) 850, 1543, 2467
AsuII TTCGAA 1 cut(s) 3192
AsuNHI GCTAGC 1 cut(s) 1739
AvaI CYCGRG 1 cut(s) 761
AvaII GGWCC 4 cut(s) 212, 920, 3005, 3089
AxyI CCTNAGG 1 cut(s) 2070
BaeGI GKGCMC 3 cut(s) 566, 644, 3250
BaeI ACNNNNGTAYC 2 cut(s) 1305, 1338
BanI GGYRCC 4 cut(s) 397, 422, 1151, 1321
BanII GRGCYC 1 cut(s) 1248
BarI GAAGNNNNNNTAC 2 cut(s) 933, 965
BbsI GAAGAC 2 cut(s) 494, 1116
Bbv12I GWGCWC 2 cut(s) 1248, 3250
BbvCI CCTCAGC 1 cut(s) 1776
BbvI GCAGC 9 cut(s) 1381, 1384, 1392, 1755, 2342, 2351, 2626, 2863, 3005
BciT130I CCWGG 2 cut(s) 1115, 2453
BciVI GTATCC 2 cut(s) 868, 2596
BcoDI GTCTC 9 cut(s) 241, 449, 470, 1853, 1862, 1905, 2106, 2819, 3105
BfmI CTRYAG 3 cut(s) 1841, 2233, 3065
BfoI RGCGCY 1 cut(s) 10
BfuI GTATCC 2 cut(s) 868, 2596
BglI GCCNNNNNGGC 1 cut(s) 1548
BglII AGATCT 2 cut(s) 523, 2590
BlpI GCTNAGC 2 cut(s) 1401, 2202
BmcAI AGTACT 4 cut(s) 143, 1075, 2435, 3026
Bme1390I CCNGG 2 cut(s) 1115, 2453
Bme18I GGWCC 4 cut(s) 212, 920, 3005, 3089
BmeT110I CYCGRG 1 cut(s) 761
BmgT120I GGNCC 5 cut(s) 212, 699, 920, 3005, 3089
BmiI GGNNCC 8 cut(s) 213, 380, 399, 424, 1153, 1323, 2506, 3006
BmrFI CCNGG 2 cut(s) 1115, 2453
BmrI ACTGGG 1 cut(s) 2425
BmsI GCATC 8 cut(s) 336, 586, 775, 1175, 1369, 2313, 2601, 2626
BmtI GCTAGC 1 cut(s) 1743
BmuI ACTGGG 1 cut(s) 2425
BpiI GAAGAC 2 cut(s) 494, 1116
BpmI CTGGAG 5 cut(s) 171, 828, 1062, 1514, 1929
Bpu10I CCTNAGC 3 cut(s) 1776, 1965, 3241
Bpu1102I GCTNAGC 2 cut(s) 1401, 2202
Bpu14I TTCGAA 1 cut(s) 3192
BpuEI CTTGAG 3 cut(s) 1353, 1463, 2102
Bsa29I ATCGAT 2 cut(s) 2091, 3158
BsaAI YACGTR 2 cut(s) 1667, 3039
BsaBI GATNNNNATC 1 cut(s) 600
BsaHI GRCGYC 1 cut(s) 2186
BsaI GGTCTC 1 cut(s) 2106
BsaJI CCNNGG 4 cut(s) 923, 1114, 1482, 2451
BsaWI WCCGGW 3 cut(s) 910, 1148, 2786
BsaXI ACNNNNNCTCC 4 cut(s) 699, 729, 1060, 1090
Bsc4I CCNNNNNNNGG 4 cut(s) 832, 917, 1015, 2921
Bse118I RCCGGY 2 cut(s) 1148, 1262
Bse1I ACTGG 8 cut(s) 188, 727, 811, 1912, 2431, 2508, 2556, 2900
Bse21I CCTNAGG 1 cut(s) 2070
Bse3DI GCAATG 3 cut(s) 733, 899, 1219
Bse8I GATNNNNATC 1 cut(s) 600
BseAI TCCGGA 2 cut(s) 910, 2786
BseBI CCWGG 2 cut(s) 1115, 2453
BseCI ATCGAT 2 cut(s) 2091, 3158
BseDI CCNNGG 4 cut(s) 923, 1114, 1482, 2451
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 4 cut(s) 832, 917, 1015, 2921
BseMI GCAATG 3 cut(s) 733, 899, 1219
BseMII CTCAG 7 cut(s) 294, 1415, 1767, 1956, 2216, 2608, 3255
BseNI ACTGG 8 cut(s) 188, 727, 811, 1912, 2431, 2508, 2556, 2900
BseRI GAGGAG 2 cut(s) 1256, 2777
BseSI GKGCMC 3 cut(s) 566, 644, 3250
BseXI GCAGC 9 cut(s) 1381, 1384, 1392, 1755, 2342, 2351, 2626, 2863, 3005
BseYI CCCAGC 1 cut(s) 505
BshFI GGCC 3 cut(s) 441, 701, 3046
BshNI GGYRCC 4 cut(s) 397, 422, 1151, 1321
BshTI ACCGGT 1 cut(s) 1148
BshVI ATCGAT 2 cut(s) 2091, 3158
BsiHKAI GWGCWC 2 cut(s) 1248, 3250
BsiHKCI CYCGRG 1 cut(s) 761
BsiSI CCGG 4 cut(s) 911, 1149, 1263, 2787
BslFI GGGAC 2 cut(s) 1131, 2413
BslI CCNNNNNNNGG 4 cut(s) 832, 917, 1015, 2921
BsmAI GTCTC 9 cut(s) 241, 449, 470, 1853, 1862, 1905, 2106, 2819, 3105
BsmFI GGGAC 2 cut(s) 1131, 2413
BsmI GAATGC 1 cut(s) 1231
BsnI GGCC 3 cut(s) 441, 701, 3046
Bso31I GGTCTC 1 cut(s) 2106
BsoBI CYCGRG 1 cut(s) 761
Bsp119I TTCGAA 1 cut(s) 3192
Bsp1286I GDGCHC 4 cut(s) 566, 644, 1248, 3250
Bsp13I TCCGGA 2 cut(s) 910, 2786
Bsp1407I TGTACA 2 cut(s) 717, 1353
Bsp143I GATC 8 cut(s) 406, 481, 523, 1446, 1834, 2590, 2690, 2898
Bsp1720I GCTNAGC 2 cut(s) 1401, 2202
BspACI CCGC 5 cut(s) 127, 607, 685, 1551, 2645
BspANI GGCC 3 cut(s) 441, 701, 3046
BspCNI CTCAG 7 cut(s) 293, 1414, 1768, 1957, 2215, 2607, 3254
BspDI ATCGAT 2 cut(s) 2091, 3158
BspEI TCCGGA 2 cut(s) 910, 2786
BspHI TCATGA 2 cut(s) 132, 975
BspLI GGNNCC 8 cut(s) 213, 380, 399, 424, 1153, 1323, 2506, 3006
BspOI GCTAGC 1 cut(s) 1743
BspPI GGATC 3 cut(s) 1842, 2685, 2906
BspQI GCTCTTC 1 cut(s) 1276
BspT104I TTCGAA 1 cut(s) 3192
BspT107I GGYRCC 4 cut(s) 397, 422, 1151, 1321
BspTNI GGTCTC 1 cut(s) 2106
BsrDI GCAATG 3 cut(s) 733, 899, 1219
BsrFI RCCGGY 2 cut(s) 1148, 1262
BsrGI TGTACA 2 cut(s) 717, 1353
BsrI ACTGG 8 cut(s) 188, 727, 811, 1912, 2431, 2508, 2556, 2900
BssAI RCCGGY 2 cut(s) 1148, 1262
BssECI CCNNGG 4 cut(s) 923, 1114, 1482, 2451
BssMI GATC 8 cut(s) 406, 481, 523, 1446, 1834, 2590, 2690, 2898
BssNAI GTATAC 2 cut(s) 2931, 3036
BssNI GRCGYC 1 cut(s) 2186
BssT1I CCWWGG 1 cut(s) 1482
Bst1107I GTATAC 2 cut(s) 2931, 3036
Bst2UI CCWGG 2 cut(s) 1115, 2453
Bst4CI ACNGT 5 cut(s) 53, 1297, 1318, 2157, 2608
Bst6I CTCTTC 2 cut(s) 954, 1276
BstACI GRCGYC 1 cut(s) 2186
BstAPI GCANNNNNTGC 1 cut(s) 239
BstAUI TGTACA 2 cut(s) 717, 1353
BstBAI YACGTR 2 cut(s) 1667, 3039
BstBI TTCGAA 1 cut(s) 3192
BstC8I GCNNGC 7 cut(s) 160, 511, 1741, 1885, 2328, 2805, 3072
BstDEI CTNAG 8 cut(s) 280, 1401, 1776, 1965, 2070, 2202, 2594, 3241
BstDSI CCRYGG 1 cut(s) 923
BstENI CCTNNNNNAGG 1 cut(s) 1013
BstH2I RGCGCY 1 cut(s) 10
BstHHI GCGC 2 cut(s) 9, 1942
BstKTI GATC 8 cut(s) 409, 484, 526, 1449, 1837, 2593, 2693, 2901
BstMAI GTCTC 9 cut(s) 241, 449, 470, 1853, 1862, 1905, 2106, 2819, 3105
BstMBI GATC 8 cut(s) 406, 481, 523, 1446, 1834, 2590, 2690, 2898
BstMWI GCNNNNNNNGC 6 cut(s) 239, 785, 1548, 1583, 2336, 2339
BstNI CCWGG 2 cut(s) 1115, 2453
BstNSI RCATGY 2 cut(s) 1605, 2735
BstSCI CCNGG 2 cut(s) 1113, 2451
BstSFI CTRYAG 3 cut(s) 1841, 2233, 3065
BstSLI GKGCMC 3 cut(s) 566, 644, 3250
BstSNI TACGTA 2 cut(s) 1667, 3039
BstV1I GCAGC 9 cut(s) 1381, 1384, 1392, 1755, 2342, 2351, 2626, 2863, 3005
BstV2I GAAGAC 2 cut(s) 494, 1116
BstX2I RGATCY 3 cut(s) 523, 2590, 2898
BstXI CCANNNNNNTGG 1 cut(s) 2967
BstYI RGATCY 3 cut(s) 523, 2590, 2898
BstZ17I GTATAC 2 cut(s) 2931, 3036
Bsu15I ATCGAT 2 cut(s) 2091, 3158
Bsu36I CCTNAGG 1 cut(s) 2070
BsuI GTATCC 2 cut(s) 868, 2596
BsuRI GGCC 3 cut(s) 441, 701, 3046
BsuTUI ATCGAT 2 cut(s) 2091, 3158
BtgI CCRYGG 1 cut(s) 923
BtgZI GCGATG 1 cut(s) 21
BtsI GCAGTG 1 cut(s) 720
BtsIMutI CAGTG 7 cut(s) 720, 720, 1323, 2314, 2581, 2602, 2911
Cac8I GCNNGC 7 cut(s) 160, 511, 1741, 1885, 2328, 2805, 3072
CaiI CAGNNNCTG 1 cut(s) 239
CciI TCATGA 2 cut(s) 132, 975
CfoI GCGC 2 cut(s) 9, 1942
Cfr10I RCCGGY 2 cut(s) 1148, 1262
Cfr13I GGNCC 5 cut(s) 212, 699, 920, 3005, 3089
ClaI ATCGAT 2 cut(s) 2091, 3158
CseI GACGC 1 cut(s) 2194
CspAI ACCGGT 1 cut(s) 1148
DdeI CTNAG 8 cut(s) 280, 1401, 1776, 1965, 2070, 2202, 2594, 3241
DpnI GATC 8 cut(s) 408, 483, 525, 1448, 1836, 2592, 2692, 2900
DpnII GATC 8 cut(s) 406, 481, 523, 1446, 1834, 2590, 2690, 2898
DraI TTTAAA 1 cut(s) 2220
Eam1104I CTCTTC 2 cut(s) 954, 1276
EarI CTCTTC 2 cut(s) 954, 1276
Ecl136II GAGCTC 1 cut(s) 1246
Eco105I TACGTA 2 cut(s) 1667, 3039
Eco130I CCWWGG 1 cut(s) 1482
Eco147I AGGCCT 1 cut(s) 441
Eco24I GRGCYC 1 cut(s) 1248
Eco31I GGTCTC 1 cut(s) 2106
Eco32I GATATC 1 cut(s) 275
Eco47I GGWCC 4 cut(s) 212, 920, 3005, 3089
Eco47III AGCGCT 1 cut(s) 8
Eco53kI GAGCTC 1 cut(s) 1246
Eco57I CTGAAG 9 cut(s) 149, 450, 506, 653, 2025, 2046, 2887, 3013, 3041
Eco81I CCTNAGG 1 cut(s) 2070
Eco88I CYCGRG 1 cut(s) 761
EcoICRI GAGCTC 1 cut(s) 1246
EcoNI CCTNNNNNAGG 1 cut(s) 1013
EcoRII CCWGG 2 cut(s) 1113, 2451
EcoRV GATATC 1 cut(s) 275
EcoT14I CCWWGG 1 cut(s) 1482
EcoT38I GRGCYC 1 cut(s) 1248
ErhI CCWWGG 1 cut(s) 1482
FalI AAGNNNNNCTT 4 cut(s) 1965, 1997, 2190, 2222
FaqI GGGAC 2 cut(s) 1131, 2413
FauI CCCGC 2 cut(s) 1544, 2638
FauNDI CATATG 1 cut(s) 1410
FblI GTMKAC 3 cut(s) 2930, 2953, 3035
FriOI GRGCYC 1 cut(s) 1248
GlaI GCGC 2 cut(s) 8, 1941
GsaI CCCAGC 1 cut(s) 509
GsuI CTGGAG 5 cut(s) 171, 828, 1062, 1514, 1929
HaeII RGCGCY 1 cut(s) 10
HaeIII GGCC 3 cut(s) 441, 701, 3046
HapII CCGG 4 cut(s) 911, 1149, 1263, 2787
HgaI GACGC 1 cut(s) 2194
HhaI GCGC 2 cut(s) 9, 1942
Hin1I GRCGYC 1 cut(s) 2186
Hin6I GCGC 2 cut(s) 7, 1940
HinP1I GCGC 2 cut(s) 7, 1940
HincII GTYRAC 5 cut(s) 804, 1642, 1756, 1905, 3285
HindII GTYRAC 5 cut(s) 804, 1642, 1756, 1905, 3285
HindIII AAGCTT 3 cut(s) 770, 2133, 2805
HinfI GANTC 3 cut(s) 32, 136, 3010
HpaII CCGG 4 cut(s) 911, 1149, 1263, 2787
HphI GGTGA 3 cut(s) 850, 1543, 2467
HpyAV CCTTC 9 cut(s) 173, 283, 865, 911, 1009, 1528, 2312, 2890, 3222
HpyCH4III ACNGT 5 cut(s) 53, 1297, 1318, 2157, 2608
HpyCH4IV ACGT 4 cut(s) 1285, 1666, 3038, 3287
HpyF10VI GCNNNNNNNGC 6 cut(s) 239, 785, 1548, 1583, 2336, 2339
HpyF3I CTNAG 8 cut(s) 280, 1401, 1776, 1965, 2070, 2202, 2594, 3241
HpySE526I ACGT 4 cut(s) 1285, 1666, 3038, 3287
Hsp92I GRCGYC 1 cut(s) 2186
HspAI GCGC 2 cut(s) 7, 1940
Kpn2I TCCGGA 2 cut(s) 910, 2786
KpnI GGTACC 2 cut(s) 1155, 1325
Kzo9I GATC 8 cut(s) 406, 481, 523, 1446, 1834, 2590, 2690, 2898
LguI GCTCTTC 1 cut(s) 1276
LmnI GCTCC 5 cut(s) 384, 809, 1243, 1574, 3108
Lsp1109I GCAGC 9 cut(s) 1381, 1384, 1392, 1755, 2342, 2351, 2626, 2863, 3005
LweI GCATC 8 cut(s) 336, 586, 775, 1175, 1369, 2313, 2601, 2626
MaeII ACGT 4 cut(s) 1285, 1666, 3038, 3287
MaeIII GTNAC 8 cut(s) 53, 174, 365, 677, 1819, 2182, 2228, 2399
MalI GATC 8 cut(s) 408, 483, 525, 1448, 1836, 2592, 2692, 2900
MboI GATC 8 cut(s) 406, 481, 523, 1446, 1834, 2590, 2690, 2898
MfeI CAATTG 1 cut(s) 1863
MflI RGATCY 3 cut(s) 523, 2590, 2898
MhlI GDGCHC 4 cut(s) 566, 644, 1248, 3250
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 2939
MroI TCCGGA 2 cut(s) 910, 2786
MroXI GAANNNNTTC 2 cut(s) 1587, 3026
MslI CAYNNNNRTG 1 cut(s) 2736
MspA1I CMGCKG 3 cut(s) 779, 1405, 2342
MspI CCGG 4 cut(s) 911, 1149, 1263, 2787
MspR9I CCNGG 2 cut(s) 1115, 2453
MunI CAATTG 1 cut(s) 1863
Mva1269I GAATGC 1 cut(s) 1231
MvaI CCWGG 2 cut(s) 1115, 2453
MwoI GCNNNNNNNGC 6 cut(s) 239, 785, 1548, 1583, 2336, 2339
NdeI CATATG 1 cut(s) 1410
NdeII GATC 8 cut(s) 406, 481, 523, 1446, 1834, 2590, 2690, 2898
NheI GCTAGC 1 cut(s) 1739
NlaIV GGNNCC 8 cut(s) 213, 380, 399, 424, 1153, 1323, 2506, 3006
NmuCI GTSAC 4 cut(s) 53, 174, 2182, 2228
NspI RCATGY 2 cut(s) 1605, 2735
NspV TTCGAA 1 cut(s) 3192
PagI TCATGA 2 cut(s) 132, 975
PceI AGGCCT 1 cut(s) 441
PciSI GCTCTTC 1 cut(s) 1276
PctI GAATGC 1 cut(s) 1231
PdmI GAANNNNTTC 2 cut(s) 1587, 3026
PfeI GAWTC 2 cut(s) 136, 3010
PflMI CCANNNNNTGG 1 cut(s) 832
PinAI ACCGGT 1 cut(s) 1148
PleI GAGTC 1 cut(s) 40
PpsI GAGTC 1 cut(s) 40
Ppu21I YACGTR 2 cut(s) 1667, 3039
PshBI ATTAAT 1 cut(s) 2381
PsiI TTATAA 2 cut(s) 2445, 2541
Psp124BI GAGCTC 1 cut(s) 1248
Psp6I CCWGG 2 cut(s) 1113, 2451
PspFI CCCAGC 1 cut(s) 505
PspGI CCWGG 2 cut(s) 1113, 2451
PspN4I GGNNCC 8 cut(s) 213, 380, 399, 424, 1153, 1323, 2506, 3006
PspPI GGNCC 5 cut(s) 212, 699, 920, 3005, 3089
PstNI CAGNNNCTG 1 cut(s) 239
PsuI RGATCY 3 cut(s) 523, 2590, 2898
PvuII CAGCTG 3 cut(s) 779, 1405, 2342
RseI CAYNNNNRTG 1 cut(s) 2736
SacI GAGCTC 1 cut(s) 1248
SapI GCTCTTC 1 cut(s) 1276
Sau3AI GATC 8 cut(s) 406, 481, 523, 1446, 1834, 2590, 2690, 2898
Sau96I GGNCC 5 cut(s) 212, 699, 920, 3005, 3089
ScaI AGTACT 4 cut(s) 143, 1075, 2435, 3026
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 2 cut(s) 1115, 2453
SduI GDGCHC 4 cut(s) 566, 644, 1248, 3250
SfaNI GCATC 8 cut(s) 336, 586, 775, 1175, 1369, 2313, 2601, 2626
SfcI CTRYAG 3 cut(s) 1841, 2233, 3065
SfuI TTCGAA 1 cut(s) 3192
SgrAI CRCCGGYG 1 cut(s) 1262
SinI GGWCC 4 cut(s) 212, 920, 3005, 3089
SmiMI CAYNNNNRTG 1 cut(s) 2736
SmlI CTYRAG 3 cut(s) 1368, 1442, 2117
SmoI CTYRAG 3 cut(s) 1368, 1442, 2117
SnaBI TACGTA 2 cut(s) 1667, 3039
SseBI AGGCCT 1 cut(s) 441
SsiI CCGC 5 cut(s) 127, 607, 685, 1551, 2645
SspI AATATT 1 cut(s) 2979
SstI GAGCTC 1 cut(s) 1248
StuI AGGCCT 1 cut(s) 441
StyD4I CCNGG 2 cut(s) 1113, 2451
StyI CCWWGG 1 cut(s) 1482
TaaI ACNGT 5 cut(s) 53, 1297, 1318, 2157, 2608
TaiI ACGT 4 cut(s) 1288, 1669, 3041, 3290
TaqI TCGA 7 cut(s) 952, 1005, 1463, 2091, 3158, 3192, 3255
TatI WGTACW 9 cut(s) 78, 141, 203, 717, 1073, 1128, 1353, 2433, 3024
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 2 cut(s) 136, 3010
TscAI CASTG 7 cut(s) 720, 727, 1323, 2314, 2581, 2602, 2911
TseFI GTSAC 4 cut(s) 53, 174, 2182, 2228
TseI GCWGC 9 cut(s) 1394, 1397, 1405, 1743, 2330, 2339, 2614, 2876, 2993
Tsp45I GTSAC 4 cut(s) 53, 174, 2182, 2228
TspGWI ACGGA 2 cut(s) 912, 3141
TspRI CASTG 7 cut(s) 720, 727, 1323, 2314, 2581, 2602, 2911
Van91I CCANNNNNTGG 1 cut(s) 832
VneI GTGCAC 1 cut(s) 3246
VpaK11BI GGWCC 4 cut(s) 212, 920, 3005, 3089
VspI ATTAAT 1 cut(s) 2381
XagI CCTNNNNNAGG 1 cut(s) 1013
XceI RCATGY 2 cut(s) 1605, 2735
XcmI CCANNNNNNNNNTGG 1 cut(s) 108
XmiI GTMKAC 3 cut(s) 2930, 2953, 3035
XmnI GAANNNNTTC 2 cut(s) 1587, 3026
ZrmI AGTACT 4 cut(s) 143, 1075, 2435, 3026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.