Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0006243

Physical Location & Seq

Genomic Coordinates Forward (+)

24135501 .. 24150534

Gene Structure 15034 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002001

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3195 bp

ATGAAGCGCTTCATCTACATAAACGATGATGATTCATCGCACTATCCTTACTGTGACAATCGTATATCGAATAGAAAGTACACTGTGTTGAACTTTCTCCCCAAGAATTTATGGGAACAATTCAGCCGCTTCATGAATCAGTACTTTCTGTTGATTGCTTGCCTTCAGCTATGGTCACTTATTACTCCAGTAAATCCTGCCAGCACATGGGGTCCACTTATCTTCATTTTTGCAGTCTCTGCATCAAAAGAGGCCTGGGATGATTACAACCGATATCTCTCAGACAAGAAGGCAAATGAGAAAGAAGTTTGGGTCGTCAGGCATGGCATCAAAAAACATATACAAGCGCAAGATATTCGTCTTGGTAACATAGTTTGGCTGCGGGAGAATGATGAAGTGCCATGTGACCTAGTTTTGATTGGTACCTCAGAAGCACAAGGCCTATGCTATATAGAGACTGCAGCCCTAGATGGAGAAACTGATCTGAAGACCAGGGTCATACCACCAGCTTGTATGGGGATAGATCTTGAATTGTTGCACAAAATTAAGGGTGTAATTGAGTGCCCTAATCCTGATAAGGACATTCGAAGATTTGATGCAAATATGCGGTTGTTTCCCCCATTTATTGATAATGACCTCTGCCCATTAACCATAAAGAATACTATTCTTCAGTCATGTTACTTGCGAAATACAGAGTGGGCCTGTGGAGTAGCAGTCTACACTGGCAATGAAACCAAGCTTGGTATGACCAGGGGCATTCCTGAGCCAAAGCTTACAGCTGTCGATGCTATGATTGACAAGTTGACAGGAGCTATTTTTGTTTTCCAAGTAGTGGTGGTTATGGTTCTAGGCGTTGCTGGTAATGTCTGGAAGGATACAGAAGCAAGGAAGCAATGGTATGTTCTATATCCAGAGGAAGGTCCTTGGTACGAACTATTAGTAATCCCTCTTCGCTTTGAACTACTTTGTTCCATCATGATACCCATATCAATTAAGGTATCTCTCGACCTTGTCAAGAGCTTGTATGCAAAGTTTATTGATTGGGATAGTAAAATGATTGACCAAGAAACGTCTACTCCAGCCCATGCAGCAAATACAGCAATAAGTGAAGACCTGGGACAAGTTGAGTACATTCTGACTGATAAAACTGGTACTCTAACGGAAAATAAAATGATATTTAGAAGGTGTTGTATCAATGGCATTTACTATGGAAATGAGAATGGCAATGCCTTAAAAGATGAGGAGCTCATTGATGCTATTTCTAGTGGCGCTTCTGATGTTATACGTTTCCTTACAGTTATGGCGATATGTAATACAGTCATACCCATTCAAAGCAAAAATGGAAGTATCGTGTACAAGGCACAATCTCAAGATGAAGATGCTCTTGTTCATGCTGCTTCTCAGCTACATATGGTTTTTGTCAATAAAAATGCTAACATTCTTGAGATCAAATTCAACGGTTCTACAGTTCAGTATGAAGCCTTGGATATTCTGGAGTTCACTTCTGATAGAAAAAGAATGTCAGTAGTGGTAAAGGATTGTCACAATGGAAGGATTATTCTTTTATCGAAGGGAGCAGATGAAGCAATTCTTCCTTATGCATGTGCTGGACAGCAAACAAGGACTGTTATTGAAGCTGTTGAGCAATATGCTCAGTTGGGACTACGGACACTGTGTCTAGCTTGGCGTGAACTAAAAGAAGAGGAATATCGAGAATGGTCTCTGATGTATAAAGAGGCTAGCAGCACATTGGTTGATAGGGAGTGGAGGCTAGCAGAGGTCTGTCAAAGATTAGAACATGATTTTGAAATACTTGGAGTTACTGCAATAGAGGATCGCCTACAGCCAAAAGGCCAACTTTTGTTACTTGATGGGAAAACTGAAGATGAAGTTCGTAGAAGTTTGGAGAGAGTTTTACTCACAATGCGGACAACAACTTCAGAACCTAAGGATGTGGCGTTTGTCATTGATGGCTGGTCCCTTGAAATTGCACTGAAGCACTATCGGAAATCTTTTACTGAGTTGGCAATATTGTCAAGGACTGCTATATGTTGTCGTGTGACACCATCACAGAAAGCTCAGCTTGTGGAGATTTTAAAATCGTGTGACTATAGAACATTGGCCATTGGGGATGGTGGGAATGATGTGAGAATGATACAACAAGCTGACATTGGTGTTGGCATTAGTGGGAGAGAAGGACTGCAGGCAGCTAGGGCTGCTGATTATAGTATTGGGAAGTTCAGGTTCCTGAAAAGATTGATACTTGTTCATGGGCGTTACTCATACAACCGCACAGCGTTTCTGTCCCAGTATTCTTTTTATAAGTCATTGGTCGTATGTTTCATCCAGATTTTTTTCTCTTTTATTTCAGGGGTCTCTGGAACCAGTCTTTTCAATTCTGTTAGCTTGATGGCTTATAATGTCTTCTACACCAGTGTTCCTGTTCTAGCCAGCGTCCTTGATAAAGATCTTAGTGAGGAAACTGTTATGCAGCATCCACAGATTCTATTCTATTGCCAAGCGGGAAGGCTTCTGAATCCTAGCACATTTGCTGGATGGTTTGGGCGATCCCTTTTCCACGCAATTGTTGTATTTGTGATCAGCATACATGCTTATGCCTATGAAAAAAGTGAAATGGAGGAGGTTTCAATGGTGGCGCTCTCTGGATGTATTTGGTTGCAGGCTTTTGTAATGACATTAGAGACAAGCTCCTTTACTATATTGCAACATCTTGCTATATGGGGGAATTTAGCTGCCTTCTACATCATCAACTGGATCTTCAGTGCCATTCCAGGATCAGGGATGTACACAATTATGTTTCGCTTGTGTATAGAGCCTTCTTACTGGATAACAATCTTGCTCATAGTTGCAGCAGGAATGGGCCCAATTCTAGCTTTGAAGTACTTCAGGTATACATACAGACCAAGCAAAATCAATACGCTACAGCAAGCTGAACGTTTGGGCGGGCCTATCCTGTCTATTGGGAGCATTGAGCCTCAAACAAGAAGCATAGAAAATGAAGTTTCTCCATTATCAATTACTCAACCGAAGAACAGAAATCCAATTTTCGAACCTCTATTATCAGATTCACCCAATGCTACACGAAGATCTTTTGGTTCAGGAACACCTTTTGATTTCTTTCAATCACAGTCCAGATTGTCCATGTCTAACTACTCAAGAAACAAGGACAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0008150 GO:0009987 GO:0010876 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0033036 GO:0034204 GO:0042623 GO:0043492 GO:0044464 GO:0045332 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase Hydrolase Hydrolase_3 PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1065

Amino Acids

120.5

Weight (kDa)

5.97

Isoelectric Point (pI)

43.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	5 - 70	2.9e-24	Phospholipid-translocating ATPase N-terminal
E1-E2_ATPase	PF00122	100 - 162	3.1e-06	P-type ATPase actuator domain
Cation_ATPase	PF13246	427 - 616	1.2e-16	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	737 - 976	1.4e-64	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G44240 AT5G44240
fragaria_vesca	FvH4_5g29681 FvH4_5g29681 FvH4_5g29681 FvH4_5g29681 FvH4_5g29681 FvH4_5g29681
malus_domestica	MD09G1243400.v1.1 MD17G1235400.v1.1
prunus_persica	Prupe.3G133300_v2.0.a1 Prupe.3G133300_v2.0.a1 Prupe.3G133300_v2.0.a1
pyrus_communis	pycom09g16200 pycom17g23850
rosa_chinensis	RchiOBHm_Chr7g0223111
rosa_laevigata	RLG00000002001 RLG00000002345
rosa_multiflora	Rmu_sc0006373.1_g000014
rosa_roxburghii	Rroxscaffold_3G00236330
rosa_rugosa	Rorug07G0212900 Rorug07G0212900
rosa_samantha	Rh7AG358200 Rh7BG346300 Rh7CG376500 Rh7DG353600
rosa_wichuraiana	Rw7G030480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2322, 2418
Acc65I	GGTACC	1 cut(s)	422
AccB1I	GGYRCC	1 cut(s)	422
AccB7I	CCANNNNNTGG	2 cut(s)	207, 832
AccI	GTMKAC	3 cut(s)	717, 1073, 2912
AciI	CCGC	7 cut(s)	127, 382, 607, 1927, 2290, 2522, 2964
AclI	AACGTT	1 cut(s)	2956
AclWI	GGATC	4 cut(s)	1842, 2562, 2783, 2803
AcoI	YGGCCR	1 cut(s)	2121
AcsI	RAATTY	3 cut(s)	106, 1451, 2746
AcuI	CTGAAG	8 cut(s)	149, 506, 653, 1902, 1923, 2015, 2764, 2890
AfaI	GTAC	9 cut(s)	80, 143, 424, 929, 1130, 1153, 1355, 2807, 2903
AfeI	AGCGCT	1 cut(s)	8
AfiI	CCNNNNNNNGG	4 cut(s)	207, 832, 917, 2798
AhdI	GACNNNNNGTC	1 cut(s)	1674
AjnI	CCWGG	5 cut(s)	254, 491, 749, 1113, 2791
AjuI	GAANNNNNNNTTGG	2 cut(s)	723, 755
Alw21I	GWGCWC	1 cut(s)	1248
Alw26I	GTCTC	5 cut(s)	241, 449, 1725, 2380, 2696
AlwI	GGATC	4 cut(s)	1842, 2562, 2783, 2803
AlwNI	CAGNNNCTG	1 cut(s)	239
Aor51HI	AGCGCT	1 cut(s)	8
AoxI	GGCC	7 cut(s)	252, 439, 699, 1852, 2121, 2881, 2966
ApaI	GGGCCC	1 cut(s)	2885
ApoI	RAATTY	3 cut(s)	106, 1451, 2746
Asp700I	GAANNNNTTC	3 cut(s)	8, 1587, 2903
Asp718I	GGTACC	1 cut(s)	422
AspLEI	GCGC	4 cut(s)	9, 349, 1271, 2659
AspS9I	GGNCC	7 cut(s)	212, 699, 920, 1977, 2881, 2882, 2966
AsuHPI	GGTGA	1 cut(s)	3081
AsuII	TTCGAA	2 cut(s)	586, 3069
AsuNHI	GCTAGC	2 cut(s)	1739, 1771
AvaII	GGWCC	3 cut(s)	212, 920, 1977
AxyI	CCTNAGG	1 cut(s)	1947
BaeGI	GKGCMC	2 cut(s)	566, 2885
BalI	TGGCCA	1 cut(s)	2123
BanI	GGYRCC	1 cut(s)	422
BanII	GRGCYC	2 cut(s)	1248, 2885
BarI	GAAGNNNNNNTAC	4 cut(s)	933, 965, 2821, 2853
BbsI	GAAGAC	3 cut(s)	494, 1116, 2416
Bbv12I	GWGCWC	1 cut(s)	1248
BccI	CCATC	8 cut(s)	464, 980, 1865, 1964, 2074, 2126, 2404, 2550
BcgI	CGANNNNNNTGC	2 cut(s)	338, 372
BciT130I	CCWGG	5 cut(s)	256, 493, 751, 1115, 2793
BciVI	GTATCC	1 cut(s)	868
BclI	TGATCA	1 cut(s)	2598
BcoDI	GTCTC	5 cut(s)	241, 449, 1725, 2380, 2696
BfmI	CTRYAG	6 cut(s)	459, 1464, 1841, 2110, 2201, 2942
BfoI	RGCGCY	3 cut(s)	10, 1272, 2660
BfuI	GTATCC	1 cut(s)	868
BglII	AGATCT	3 cut(s)	523, 2467, 3107
BlpI	GCTNAGC	1 cut(s)	2079
BmcAI	AGTACT	2 cut(s)	143, 2903
Bme1390I	CCNGG	5 cut(s)	256, 493, 751, 1115, 2793
Bme18I	GGWCC	3 cut(s)	212, 920, 1977
BmeRI	GACNNNNNGTC	1 cut(s)	1674
BmgT120I	GGNCC	7 cut(s)	212, 699, 920, 1977, 2881, 2882, 2966
BmiI	GGNNCC	6 cut(s)	213, 424, 1979, 2246, 2383, 2883
BmrFI	CCNGG	5 cut(s)	256, 493, 751, 1115, 2793
BmrI	ACTGGG	1 cut(s)	2302
BmsI	GCATC	7 cut(s)	251, 336, 586, 775, 1243, 1369, 2503
BmtI	GCTAGC	2 cut(s)	1743, 1775
BmuI	ACTGGG	1 cut(s)	2302
BoxI	GACNNNNGTC	1 cut(s)	494
BpiI	GAAGAC	3 cut(s)	494, 1116, 2416
BplI	GAGNNNNNCTC	4 cut(s)	1902, 1934, 2693, 2725
BpmI	CTGGAG	3 cut(s)	171, 1062, 1514
Bpu10I	CCTNAGC	1 cut(s)	762
Bpu1102I	GCTNAGC	1 cut(s)	2079
Bpu14I	TTCGAA	2 cut(s)	586, 3069
BpuEI	CTTGAG	3 cut(s)	1353, 1463, 3160
BsaBI	GATNNNNATC	2 cut(s)	2466, 2852
BsaI	GGTCTC	2 cut(s)	1725, 2380
BsaJI	CCNNGG	6 cut(s)	255, 492, 750, 923, 1114, 1482
BsaXI	ACNNNNNCTCC	4 cut(s)	699, 729, 1060, 1090
Bsc4I	CCNNNNNNNGG	4 cut(s)	207, 832, 917, 2798
Bse1I	ACTGG	8 cut(s)	188, 727, 1153, 2308, 2385, 2433, 2777, 2849
Bse21I	CCTNAGG	1 cut(s)	1947
Bse3DI	GCAATG	3 cut(s)	733, 899, 1231
Bse8I	GATNNNNATC	2 cut(s)	2466, 2852
BseBI	CCWGG	5 cut(s)	256, 493, 751, 1115, 2793
BseDI	CCNNGG	6 cut(s)	255, 492, 750, 923, 1114, 1482
BseGI	GGATG	8 cut(s)	265, 1957, 2137, 2343, 2494, 2561, 2672, 2808
BseJI	GATNNNNATC	2 cut(s)	2466, 2852
BseLI	CCNNNNNNNGG	4 cut(s)	207, 832, 917, 2798
BseMI	GCAATG	3 cut(s)	733, 899, 1231
BseMII	CTCAG	7 cut(s)	294, 441, 753, 1415, 1667, 2010, 2093
BseNI	ACTGG	8 cut(s)	188, 727, 1153, 2308, 2385, 2433, 2777, 2849
BseRI	GAGGAG	2 cut(s)	1256, 2654
BseSI	GKGCMC	2 cut(s)	566, 2885
BshFI	GGCC	7 cut(s)	254, 441, 701, 1854, 2123, 2883, 2968
BshNI	GGYRCC	1 cut(s)	422
BsiHKAI	GWGCWC	1 cut(s)	1248
BslFI	GGGAC	4 cut(s)	1131, 1674, 1963, 2290
BslI	CCNNNNNNNGG	4 cut(s)	207, 832, 917, 2798
BsmAI	GTCTC	5 cut(s)	241, 449, 1725, 2380, 2696
BsmFI	GGGAC	4 cut(s)	1131, 1674, 1963, 2290
BsmI	GAATGC	1 cut(s)	756
BsnI	GGCC	7 cut(s)	254, 441, 701, 1854, 2123, 2883, 2968
Bso31I	GGTCTC	2 cut(s)	1725, 2380
Bsp119I	TTCGAA	2 cut(s)	586, 3069
Bsp120I	GGGCCC	1 cut(s)	2881
Bsp1286I	GDGCHC	3 cut(s)	566, 1248, 2885
Bsp1407I	TGTACA	2 cut(s)	1353, 2805
Bsp1720I	GCTNAGC	1 cut(s)	2079
BspACI	CCGC	7 cut(s)	127, 382, 607, 1927, 2290, 2522, 2964
BspANI	GGCC	7 cut(s)	254, 441, 701, 1854, 2123, 2883, 2968
BspCNI	CTCAG	7 cut(s)	293, 440, 754, 1414, 1666, 2011, 2092
BspHI	TCATGA	2 cut(s)	132, 975
BspLI	GGNNCC	6 cut(s)	213, 424, 1979, 2246, 2383, 2883
BspMAI	CTGCAG	2 cut(s)	463, 2205
BspOI	GCTAGC	2 cut(s)	1743, 1775
BspPI	GGATC	4 cut(s)	1842, 2562, 2783, 2803
BspT104I	TTCGAA	2 cut(s)	586, 3069
BspT107I	GGYRCC	1 cut(s)	422
BspTNI	GGTCTC	2 cut(s)	1725, 2380
BsrDI	GCAATG	3 cut(s)	733, 899, 1231
BsrGI	TGTACA	2 cut(s)	1353, 2805
BsrI	ACTGG	8 cut(s)	188, 727, 1153, 2308, 2385, 2433, 2777, 2849
BssECI	CCNNGG	6 cut(s)	255, 492, 750, 923, 1114, 1482
BssNAI	GTATAC	1 cut(s)	2913
BssT1I	CCWWGG	2 cut(s)	923, 1482
Bst1107I	GTATAC	1 cut(s)	2913
Bst2UI	CCWGG	5 cut(s)	256, 493, 751, 1115, 2793
Bst6I	CTCTTC	2 cut(s)	954, 1695
BstAPI	GCANNNNNTGC	1 cut(s)	239
BstAUI	TGTACA	2 cut(s)	1353, 2805
BstBI	TTCGAA	2 cut(s)	586, 3069
BstC8I	GCNNGC	9 cut(s)	160, 202, 1741, 1773, 2205, 2452, 2682, 2949, 2966
BstDEI	CTNAG	9 cut(s)	280, 427, 762, 1401, 1653, 1947, 2019, 2079, 2471
BstF5I	GGATG	8 cut(s)	265, 1957, 2137, 2343, 2494, 2561, 2672, 2808
BstH2I	RGCGCY	3 cut(s)	10, 1272, 2660
BstHHI	GCGC	4 cut(s)	9, 349, 1271, 2659
BstMAI	GTCTC	5 cut(s)	241, 449, 1725, 2380, 2696
BstMWI	GCNNNNNNNGC	7 cut(s)	239, 785, 1088, 1097, 1583, 2213, 2216
BstNI	CCWGG	5 cut(s)	256, 493, 751, 1115, 2793
BstNSI	RCATGY	2 cut(s)	1605, 2612
BstPAI	GACNNNNGTC	1 cut(s)	494
BstSCI	CCNGG	5 cut(s)	254, 491, 749, 1113, 2791
BstSFI	CTRYAG	6 cut(s)	459, 1464, 1841, 2110, 2201, 2942
BstSLI	GKGCMC	2 cut(s)	566, 2885
BstV2I	GAAGAC	3 cut(s)	494, 1116, 2416
BstX2I	RGATCY	4 cut(s)	523, 2467, 2775, 3107
BstYI	RGATCY	4 cut(s)	523, 2467, 2775, 3107
BstZ17I	GTATAC	1 cut(s)	2913
Bsu36I	CCTNAGG	1 cut(s)	1947
BsuI	GTATCC	1 cut(s)	868
BsuRI	GGCC	7 cut(s)	254, 441, 701, 1854, 2123, 2883, 2968
BtgZI	GCGATG	1 cut(s)	21
BtsCI	GGATG	8 cut(s)	265, 1957, 2137, 2343, 2494, 2561, 2672, 2808
BtsIMutI	CAGTG	6 cut(s)	81, 720, 1670, 1991, 2440, 2788
Cac8I	GCNNGC	9 cut(s)	160, 202, 1741, 1773, 2205, 2452, 2682, 2949, 2966
CaiI	CAGNNNCTG	1 cut(s)	239
CciI	TCATGA	2 cut(s)	132, 975
CfoI	GCGC	4 cut(s)	9, 349, 1271, 2659
Cfr13I	GGNCC	7 cut(s)	212, 699, 920, 1977, 2881, 2882, 2966
CseI	GACGC	1 cut(s)	2443
Csp6I	GTAC	9 cut(s)	79, 142, 423, 928, 1129, 1152, 1354, 2806, 2902
CviQI	GTAC	9 cut(s)	79, 142, 423, 928, 1129, 1152, 1354, 2806, 2902
DdeI	CTNAG	9 cut(s)	280, 427, 762, 1401, 1653, 1947, 2019, 2079, 2471
DraI	TTTAAA	1 cut(s)	2097
DriI	GACNNNNNGTC	1 cut(s)	1674
EaeI	YGGCCR	1 cut(s)	2121
Eam1104I	CTCTTC	2 cut(s)	954, 1695
Eam1105I	GACNNNNNGTC	1 cut(s)	1674
EarI	CTCTTC	2 cut(s)	954, 1695
Ecl136II	GAGCTC	1 cut(s)	1246
Eco130I	CCWWGG	2 cut(s)	923, 1482
Eco147I	AGGCCT	2 cut(s)	254, 441
Eco24I	GRGCYC	2 cut(s)	1248, 2885
Eco31I	GGTCTC	2 cut(s)	1725, 2380
Eco32I	GATATC	1 cut(s)	275
Eco47I	GGWCC	3 cut(s)	212, 920, 1977
Eco47III	AGCGCT	1 cut(s)	8
Eco53kI	GAGCTC	1 cut(s)	1246
Eco57I	CTGAAG	8 cut(s)	149, 506, 653, 1902, 1923, 2015, 2764, 2890
Eco81I	CCTNAGG	1 cut(s)	1947
EcoICRI	GAGCTC	1 cut(s)	1246
EcoO109I	RGGNCCY	1 cut(s)	920
EcoRII	CCWGG	5 cut(s)	254, 491, 749, 1113, 2791
EcoRV	GATATC	1 cut(s)	275
EcoT14I	CCWWGG	2 cut(s)	923, 1482
EcoT22I	ATGCAT	1 cut(s)	1603
EcoT38I	GRGCYC	2 cut(s)	1248, 2885
ErhI	CCWWGG	2 cut(s)	923, 1482
FalI	AAGNNNNNCTT	8 cut(s)	1368, 1400, 1575, 1607, 1842, 1874, 2067, 2099
FaqI	GGGAC	4 cut(s)	1131, 1674, 1963, 2290
FauI	CCCGC	3 cut(s)	375, 2515, 2957
FauNDI	CATATG	1 cut(s)	1410
FbaI	TGATCA	1 cut(s)	2598
FblI	GTMKAC	3 cut(s)	717, 1073, 2912
FokI	GGATG	8 cut(s)	272, 1964, 2144, 2330, 2481, 2568, 2679, 2815
FriOI	GRGCYC	2 cut(s)	1248, 2885
GlaI	GCGC	4 cut(s)	8, 348, 1270, 2658
GsuI	CTGGAG	3 cut(s)	171, 1062, 1514
HaeII	RGCGCY	3 cut(s)	10, 1272, 2660
HaeIII	GGCC	7 cut(s)	254, 441, 701, 1854, 2123, 2883, 2968
HgaI	GACGC	1 cut(s)	2443
HhaI	GCGC	4 cut(s)	9, 349, 1271, 2659
Hin6I	GCGC	4 cut(s)	7, 347, 1269, 2657
HinP1I	GCGC	4 cut(s)	7, 347, 1269, 2657
HincII	GTYRAC	1 cut(s)	804
HindII	GTYRAC	1 cut(s)	804
HindIII	AAGCTT	2 cut(s)	737, 770
HinfI	GANTC	5 cut(s)	32, 136, 2503, 2536, 3086
HphI	GGTGA	1 cut(s)	3081
HpyCH4IV	ACGT	3 cut(s)	1070, 1285, 2956
HpyF10VI	GCNNNNNNNGC	7 cut(s)	239, 785, 1088, 1097, 1583, 2213, 2216
HpyF3I	CTNAG	9 cut(s)	280, 427, 762, 1401, 1653, 1947, 2019, 2079, 2471
HpySE526I	ACGT	3 cut(s)	1070, 1285, 2956
HspAI	GCGC	4 cut(s)	7, 347, 1269, 2657
KpnI	GGTACC	1 cut(s)	426
Ksp22I	TGATCA	1 cut(s)	2598
LmnI	GCTCC	5 cut(s)	809, 1243, 1574, 2714, 2985
LweI	GCATC	7 cut(s)	251, 336, 586, 775, 1243, 1369, 2503
MaeII	ACGT	3 cut(s)	1070, 1285, 2956
MfeI	CAATTG	1 cut(s)	2583
MflI	RGATCY	4 cut(s)	523, 2467, 2775, 3107
MhlI	GDGCHC	3 cut(s)	566, 1248, 2885
MlsI	TGGCCA	1 cut(s)	2123
MluNI	TGGCCA	1 cut(s)	2123
Mox20I	TGGCCA	1 cut(s)	2123
Mph1103I	ATGCAT	1 cut(s)	1603
MroXI	GAANNNNTTC	3 cut(s)	8, 1587, 2903
MscI	TGGCCA	1 cut(s)	2123
MseI	TTAA	5 cut(s)	546, 647, 993, 1232, 2096
MslI	CAYNNNNRTG	2 cut(s)	2613, 2813
Msp20I	TGGCCA	1 cut(s)	2123
MspA1I	CMGCKG	1 cut(s)	779
MspR9I	CCNGG	5 cut(s)	256, 493, 751, 1115, 2793
MunI	CAATTG	1 cut(s)	2583
Mva1269I	GAATGC	1 cut(s)	756
MvaI	CCWGG	5 cut(s)	256, 493, 751, 1115, 2793
MwoI	GCNNNNNNNGC	7 cut(s)	239, 785, 1088, 1097, 1583, 2213, 2216
NdeI	CATATG	1 cut(s)	1410
NheI	GCTAGC	2 cut(s)	1739, 1771
NlaIV	GGNNCC	6 cut(s)	213, 424, 1979, 2246, 2383, 2883
NmuCI	GTSAC	6 cut(s)	53, 174, 404, 1541, 2059, 2105
NsiI	ATGCAT	1 cut(s)	1603
NspI	RCATGY	2 cut(s)	1605, 2612
NspV	TTCGAA	2 cut(s)	586, 3069
PagI	TCATGA	2 cut(s)	132, 975
PceI	AGGCCT	2 cut(s)	254, 441
PctI	GAATGC	1 cut(s)	756
PdmI	GAANNNNTTC	3 cut(s)	8, 1587, 2903
PfeI	GAWTC	5 cut(s)	32, 136, 2503, 2536, 3086
PflFI	GACNNNGTC	1 cut(s)	1010
PflMI	CCANNNNNTGG	2 cut(s)	207, 832
PfoI	TCCNGGA	1 cut(s)	2791
PpuMI	RGGWCCY	1 cut(s)	920
PshAI	GACNNNNGTC	1 cut(s)	494
PsiI	TTATAA	2 cut(s)	2322, 2418
Psp124BI	GAGCTC	1 cut(s)	1248
Psp1406I	AACGTT	1 cut(s)	2956
Psp5II	RGGWCCY	1 cut(s)	920
Psp6I	CCWGG	5 cut(s)	254, 491, 749, 1113, 2791
PspGI	CCWGG	5 cut(s)	254, 491, 749, 1113, 2791
PspN4I	GGNNCC	6 cut(s)	213, 424, 1979, 2246, 2383, 2883
PspOMI	GGGCCC	1 cut(s)	2881
PspPI	GGNCC	7 cut(s)	212, 699, 920, 1977, 2881, 2882, 2966
PspPPI	RGGWCCY	1 cut(s)	920
PstI	CTGCAG	2 cut(s)	463, 2205
PstNI	CAGNNNCTG	1 cut(s)	239
PsuI	RGATCY	4 cut(s)	523, 2467, 2775, 3107
PsyI	GACNNNGTC	1 cut(s)	1010
PvuII	CAGCTG	1 cut(s)	779
RsaI	GTAC	9 cut(s)	80, 143, 424, 929, 1130, 1153, 1355, 2807, 2903
RsaNI	GTAC	9 cut(s)	79, 142, 423, 928, 1129, 1152, 1354, 2806, 2902
RseI	CAYNNNNRTG	2 cut(s)	2613, 2813
SacI	GAGCTC	1 cut(s)	1248
SaqAI	TTAA	5 cut(s)	546, 647, 993, 1232, 2096
Sau96I	GGNCC	7 cut(s)	212, 699, 920, 1977, 2881, 2882, 2966
ScaI	AGTACT	2 cut(s)	143, 2903
ScrFI	CCNGG	5 cut(s)	256, 493, 751, 1115, 2793
SduI	GDGCHC	3 cut(s)	566, 1248, 2885
SfaNI	GCATC	7 cut(s)	251, 336, 586, 775, 1243, 1369, 2503
SfcI	CTRYAG	6 cut(s)	459, 1464, 1841, 2110, 2201, 2942
SfuI	TTCGAA	2 cut(s)	586, 3069
SinI	GGWCC	3 cut(s)	212, 920, 1977
SmiMI	CAYNNNNRTG	2 cut(s)	2613, 2813
SmlI	CTYRAG	3 cut(s)	1368, 1442, 3175
SmoI	CTYRAG	3 cut(s)	1368, 1442, 3175
SseBI	AGGCCT	2 cut(s)	254, 441
SsiI	CCGC	7 cut(s)	127, 382, 607, 1927, 2290, 2522, 2964
SspI	AATATT	1 cut(s)	2031
SstI	GAGCTC	1 cut(s)	1248
StuI	AGGCCT	2 cut(s)	254, 441
StyD4I	CCNGG	5 cut(s)	254, 491, 749, 1113, 2791
StyI	CCWWGG	2 cut(s)	923, 1482
TaiI	ACGT	3 cut(s)	1073, 1288, 2959
TaqI	TCGA	7 cut(s)	68, 586, 783, 1005, 1568, 1711, 3069
TatI	WGTACW	6 cut(s)	78, 141, 1128, 1353, 2805, 2901
TauI	GCSGC	1 cut(s)	129
TfiI	GAWTC	5 cut(s)	32, 136, 2503, 2536, 3086
Tru1I	TTAA	5 cut(s)	546, 647, 993, 1232, 2096
Tru9I	TTAA	5 cut(s)	546, 647, 993, 1232, 2096
TscAI	CASTG	6 cut(s)	88, 727, 1677, 1998, 2440, 2788
TseFI	GTSAC	6 cut(s)	53, 174, 404, 1541, 2059, 2105
Tsp45I	GTSAC	6 cut(s)	53, 174, 404, 1541, 2059, 2105
TspGWI	ACGGA	2 cut(s)	1175, 1681
TspRI	CASTG	6 cut(s)	88, 727, 1677, 1998, 2440, 2788
Tth111I	GACNNNGTC	1 cut(s)	1010
Van91I	CCANNNNNTGG	2 cut(s)	207, 832
VpaK11BI	GGWCC	3 cut(s)	212, 920, 1977
XapI	RAATTY	3 cut(s)	106, 1451, 2746
XceI	RCATGY	2 cut(s)	1605, 2612
XmiI	GTMKAC	3 cut(s)	717, 1073, 2912
XmnI	GAANNNNTTC	3 cut(s)	8, 1587, 2903
ZrmI	AGTACT	2 cut(s)	143, 2903
Zsp2I	ATGCAT	1 cut(s)	1603

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.