MD09G1254600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013086
Physical Location & Seq
Forward (+)
32613999 .. 32616149
2151 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1254600.v1.1.491

Sequence Viewer

Length: 423 bp
ATGGGAATGGAAGGAGACAAGGAAGACGAGAGAAAGGAAGCAGCAATCGCATCCACGCCGTGTCTGCAGCCCAATTACAAGCCCAAATCCCTCACCCAAACCCAGCTATCCAAGTTCCAGGAGCTGCACCGGAGGAGGTTACAGATCAAATCAAAATCAAAATCAAAATCAAAGATTGGAAAGAAACCAAAAGGTAGTAAAGGAAAATCTCACAGCATAGACCTGGCTGCTAGAGATACTGCAAACCAAGATTCAGTCATAACATTTGAAACTTCAACTGTCTGTGACTCTGAGAACCAGAAGAATGAAACCAGTTCTGTTGTACAGCATGAGGATATAGCAGTCCGGGCTCCAAAGAAGAGCCACAAGTTACATTGGGGGCTCGACACAAAGGAAAGGTGGGAAAGGAAAGCAAACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

15.86

Weight (kDa)

9.7

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16160
fragaria_vesca FvH4_4g18360
malus_domestica MD09G1254600.v1.1
prunus_persica Prupe.3G144700_v2.0.a1 Prupe.3G144700_v2.0.a1 Prupe.3G144700_v2.0.a1
pyrus_communis pycom09g17030
rosa_chinensis RchiOBHm_Chr4g0422841
rosa_laevigata RLG00000007601
rosa_multiflora Rmu_sc0000830.1_g000043 Rmu_sc0035208.1_g000001
rosa_roxburghii Rroxscaffold_5G00364700
rosa_rugosa Rorug04G0181800
rosa_samantha Rh4AG242100 Rh4BG245700 Rh4CG256000 Rh4DG240700
rosa_wichuraiana Rw4G020850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AdeI CACNNNGTG 1 cut(s) 60
AfaI GTAC 1 cut(s) 324
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 2 cut(s) 269, 276
AjnI CCWGG 2 cut(s) 117, 222
AluBI AGCT 2 cut(s) 106, 124
AluI AGCT 2 cut(s) 106, 124
Alw26I GTCTC 1 cut(s) 9
AlwNI CAGNNNCTG 1 cut(s) 124
ApeKI GCWGC 4 cut(s) 41, 67, 124, 227
AsuC2I CCSGG 1 cut(s) 347
AsuHPI GGTGA 1 cut(s) 85
BanII GRGCYC 2 cut(s) 352, 384
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 4 cut(s) 53, 79, 111, 214
BceAI ACGGC 1 cut(s) 43
BciT130I CCWGG 2 cut(s) 119, 224
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 1 cut(s) 231
BfmI CTRYAG 1 cut(s) 65
BisI GCNGC 4 cut(s) 42, 68, 125, 228
BlsI GCNGC 4 cut(s) 43, 69, 126, 229
Bme1390I CCNGG 3 cut(s) 119, 224, 347
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 3 cut(s) 119, 224, 347
BmsI GCATC 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 30
BpuMI CCSGG 1 cut(s) 347
BsaWI WCCGGW 1 cut(s) 129
Bse1I ACTGG 1 cut(s) 312
BseBI CCWGG 2 cut(s) 119, 224
BseGI GGATG 1 cut(s) 50
BseMII CTCAG 1 cut(s) 282
BseNI ACTGG 1 cut(s) 312
BseRI GAGGAG 1 cut(s) 148
BseXI GCAGC 4 cut(s) 53, 79, 111, 214
BseYI CCCAGC 1 cut(s) 102
BsgI GTGCAG 1 cut(s) 110
BsiSI CCGG 2 cut(s) 130, 346
BsmAI GTCTC 1 cut(s) 9
Bsp1286I GDGCHC 2 cut(s) 352, 384
Bsp1407I TGTACA 1 cut(s) 322
Bsp143I GATC 1 cut(s) 144
BspCNI CTCAG 1 cut(s) 283
BspLI GGNNCC 1 cut(s) 351
BspMAI CTGCAG 1 cut(s) 69
BspQI GCTCTTC 1 cut(s) 353
BsrGI TGTACA 1 cut(s) 322
BsrI ACTGG 1 cut(s) 312
BssMI GATC 1 cut(s) 144
Bst2UI CCWGG 2 cut(s) 119, 224
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 1 cut(s) 353
BstAUI TGTACA 1 cut(s) 322
BstDEI CTNAG 1 cut(s) 291
BstF5I GGATG 1 cut(s) 50
BstKTI GATC 1 cut(s) 147
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 1 cut(s) 144
BstMWI GCNNNNNNNGC 3 cut(s) 47, 64, 347
BstNI CCWGG 2 cut(s) 119, 224
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 3 cut(s) 117, 222, 345
BstSFI CTRYAG 1 cut(s) 65
BstV1I GCAGC 4 cut(s) 53, 79, 111, 214
BstV2I GAAGAC 1 cut(s) 30
BtsCI GGATG 1 cut(s) 50
CaiI CAGNNNCTG 1 cut(s) 124
Csp6I GTAC 1 cut(s) 323
CviAII CATG 2 cut(s) 329, 418
CviJI RGCY 8 cut(s) 70, 82, 106, 124, 227, 350, 363, 382
CviKI_1 RGCY 8 cut(s) 70, 82, 106, 124, 227, 350, 363, 382
CviQI GTAC 1 cut(s) 323
DdeI CTNAG 1 cut(s) 291
DpnI GATC 1 cut(s) 146
DpnII GATC 1 cut(s) 144
DraIII CACNNNGTG 1 cut(s) 60
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
Eco24I GRGCYC 2 cut(s) 352, 384
EcoRII CCWGG 2 cut(s) 117, 222
EcoT38I GRGCYC 2 cut(s) 352, 384
FaeI CATG 2 cut(s) 332, 421
FaiI YATR 5 cut(s) 218, 260, 330, 338, 419
FatI CATG 2 cut(s) 328, 417
Fnu4HI GCNGC 4 cut(s) 42, 68, 125, 228
FokI GGATG 1 cut(s) 37
FriOI GRGCYC 2 cut(s) 352, 384
Fsp4HI GCNGC 4 cut(s) 42, 68, 125, 228
FspBI CTAG 1 cut(s) 231
GluI GCNGC 4 cut(s) 42, 68, 125, 228
GsaI CCCAGC 1 cut(s) 106
HapII CCGG 2 cut(s) 130, 346
Hin1II CATG 2 cut(s) 332, 421
HinfI GANTC 2 cut(s) 251, 287
HpaII CCGG 2 cut(s) 130, 346
HphI GGTGA 1 cut(s) 85
Hpy188I TCNGA 1 cut(s) 292
HpyAV CCTTC 1 cut(s) 5
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4V TGCA 3 cut(s) 67, 127, 242
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 64, 347
HpyF3I CTNAG 1 cut(s) 291
Hsp92II CATG 2 cut(s) 332, 421
Kzo9I GATC 1 cut(s) 144
LguI GCTCTTC 1 cut(s) 353
LmnI GCTCC 2 cut(s) 121, 355
LpnPI CCDG 9 cut(s) 104, 116, 131, 143, 209, 236, 311, 325, 359
Lsp1109I GCAGC 4 cut(s) 53, 79, 111, 214
LweI GCATC 1 cut(s) 59
MaeI CTAG 1 cut(s) 231
MaeIII GTNAC 3 cut(s) 138, 284, 369
MalI GATC 1 cut(s) 146
MboI GATC 1 cut(s) 144
MboII GAAGA 3 cut(s) 35, 313, 370
MhlI GDGCHC 2 cut(s) 352, 384
MluCI AATT 1 cut(s) 73
MlyI GAGTC 1 cut(s) 281
MnlI CCTC 4 cut(s) 101, 126, 129, 325
MspI CCGG 2 cut(s) 130, 346
MspR9I CCNGG 3 cut(s) 119, 224, 347
MvaI CCWGG 2 cut(s) 119, 224
MwoI GCNNNNNNNGC 3 cut(s) 47, 64, 347
NciI CCSGG 1 cut(s) 347
NdeII GATC 1 cut(s) 144
NlaIII CATG 2 cut(s) 332, 421
NlaIV GGNNCC 1 cut(s) 351
NmuCI GTSAC 1 cut(s) 284
NspI RCATGY 1 cut(s) 421
PciI ACATGT 1 cut(s) 417
PciSI GCTCTTC 1 cut(s) 353
PfeI GAWTC 1 cut(s) 251
PfoI TCCNGGA 1 cut(s) 117
PkrI GCNGC 4 cut(s) 43, 69, 126, 229
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PscI ACATGT 1 cut(s) 417
Psp6I CCWGG 2 cut(s) 117, 222
PspFI CCCAGC 1 cut(s) 102
PspGI CCWGG 2 cut(s) 117, 222
PspN4I GGNNCC 1 cut(s) 351
PstI CTGCAG 1 cut(s) 69
PstNI CAGNNNCTG 1 cut(s) 124
RsaI GTAC 1 cut(s) 324
RsaNI GTAC 1 cut(s) 323
SapI GCTCTTC 1 cut(s) 353
SatI GCNGC 4 cut(s) 42, 68, 125, 228
Sau3AI GATC 1 cut(s) 144
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 3 cut(s) 119, 224, 347
SduI GDGCHC 2 cut(s) 352, 384
SetI ASST 6 cut(s) 108, 126, 140, 196, 225, 401
SfaNI GCATC 1 cut(s) 59
SfcI CTRYAG 1 cut(s) 65
Sse9I AATT 1 cut(s) 73
SspMI CTAG 1 cut(s) 231
StyD4I CCNGG 3 cut(s) 117, 222, 345
TaaI ACNGT 1 cut(s) 280
TaqI TCGA 1 cut(s) 384
TasI AATT 1 cut(s) 73
TatI WGTACW 1 cut(s) 322
TfiI GAWTC 1 cut(s) 251
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 4 cut(s) 41, 67, 124, 227
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 1 cut(s) 321
XceI RCATGY 1 cut(s) 421
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.