MD09G1263000.v1.1

Protein OPI10 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006823
Physical Location & Seq
Reverse (-)
33617137 .. 33618158
1022 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1263000.v1.1.491

Sequence Viewer

Length: 564 bp
ATGTTCGGAGTCGTCTTCCCGAATCGCAGCTTCCCAATGGACATCTCCTCCTTCTCCCAGATCGACACCTTCCACTGGATTCTCGACATGAACACCTTCGTCGGGGAGGAGTACGATCAAGTGCGCGAGCTCTGCATATTTCTCTTAAACAACTTCACACTCCCGCCGGACAAAGCCCTAGCCGTCTACATCCAGTCCCCGGGCTCTGCCTTCTTCTTCTGCGGCGCCATCACCGTGGCTAGGCCGTCGGCGGTGCTTGCTCTTCCGTGGCCACCGCAGGGGGGGCAGCTGCAGCTCACAGCCGACGCGGCGCCGCTGTCGGCCAAAATCGGCGTGTCGGTGGAGGACCTGGCGACGCTGCCGTCGCTGGACGTCACCGCCGAGAAGCGAATCGAGAGGTTGGCGATGAAAGTTGGGGAGAATCTGTTCAATTTTATGCAGTCGTTTTGCGGCGTGGATGGGAGCAAGCTGGTGGTGCCCATGGATATCTTGGACCGGTGGTTCAAGAAGTTTCAGGAGAGGGCTAAGCGCGATCCTGAGTACTTGAAAGGCTTCGCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF775

Protein Analysis

188

Amino Acids

20.91

Weight (kDa)

5.02

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hikeshi-like_N PF05603 18 - 119 1.4e-09 Hikeshi-like, N-terminal domain
Hikeshi-like_C PF21057 131 - 183 2.1e-12 Hikeshi-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 361
AatII GACGTC 1 cut(s) 375
AccB1I GGYRCC 3 cut(s) 224, 310, 475
AccI GTMKAC 1 cut(s) 186
AccII CGCG 3 cut(s) 126, 308, 531
AciI CCGC 8 cut(s) 164, 222, 251, 275, 308, 314, 378, 450
AclWI GGATC 1 cut(s) 527
AcoI YGGCCR 2 cut(s) 269, 321
AcyI GRCGYC 3 cut(s) 225, 311, 372
AfaI GTAC 2 cut(s) 113, 542
AfiI CCNNNNNNNGG 6 cut(s) 75, 102, 199, 240, 278, 281
AgeI ACCGGT 1 cut(s) 495
AgsI TTSAA 3 cut(s) 430, 505, 547
AjnI CCWGG 1 cut(s) 348
AloI GAACNNNNNNTCC 4 cut(s) 410, 442, 485, 517
AluBI AGCT 5 cut(s) 30, 130, 289, 295, 469
AluI AGCT 5 cut(s) 30, 130, 289, 295, 469
Alw21I GWGCWC 1 cut(s) 132
AlwI GGATC 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 199
AoxI GGCC 3 cut(s) 242, 269, 321
ApeKI GCWGC 5 cut(s) 27, 286, 289, 292, 358
AsiGI ACCGGT 1 cut(s) 495
Asp700I GAANNNNTTC 3 cut(s) 95, 425, 551
AspLEI GCGC 4 cut(s) 126, 227, 313, 531
AspS9I GGNCC 2 cut(s) 346, 493
AsuC2I CCSGG 2 cut(s) 200, 201
AsuHPI GGTGA 2 cut(s) 223, 367
AvaI CYCGRG 1 cut(s) 199
AvaII GGWCC 2 cut(s) 346, 493
BaeGI GKGCMC 1 cut(s) 480
BalI TGGCCA 1 cut(s) 271
BanI GGYRCC 3 cut(s) 224, 310, 475
BanII GRGCYC 2 cut(s) 132, 206
BbsI GAAGAC 1 cut(s) 7
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 5 cut(s) 39, 276, 298, 304, 345
BccI CCATC 2 cut(s) 236, 452
BceAI ACGGC 3 cut(s) 167, 229, 346
BciT130I CCWGG 1 cut(s) 350
BcnI CCSGG 2 cut(s) 200, 201
BfaI CTAG 2 cut(s) 179, 240
BfmI CTRYAG 1 cut(s) 290
BfoI RGCGCY 2 cut(s) 228, 314
BglI GCCNNNNNGGC 1 cut(s) 308
BisI GCNGC 9 cut(s) 28, 223, 287, 290, 293, 309, 314, 359, 451
BlpI GCTNAGC 1 cut(s) 525
BlsI GCNGC 9 cut(s) 29, 224, 288, 291, 294, 310, 315, 360, 452
BmcAI AGTACT 1 cut(s) 542
Bme1390I CCNGG 3 cut(s) 200, 201, 350
Bme18I GGWCC 2 cut(s) 346, 493
BmeT110I CYCGRG 1 cut(s) 199
BmgT120I GGNCC 2 cut(s) 346, 493
BmiI GGNNCC 3 cut(s) 226, 312, 477
BmrFI CCNGG 3 cut(s) 200, 201, 350
BpiI GAAGAC 1 cut(s) 7
Bpu1102I GCTNAGC 1 cut(s) 525
BpuMI CCSGG 2 cut(s) 200, 201
BsaHI GRCGYC 3 cut(s) 225, 311, 372
BsaJI CCNNGG 5 cut(s) 198, 199, 234, 266, 480
BsaWI WCCGGW 1 cut(s) 495
BsaXI ACNNNNNCTCC 4 cut(s) 32, 62, 410, 440
Bsc4I CCNNNNNNNGG 6 cut(s) 75, 102, 199, 240, 278, 281
Bse118I RCCGGY 1 cut(s) 495
Bse1I ACTGG 2 cut(s) 80, 193
BseBI CCWGG 1 cut(s) 350
BseDI CCNNGG 5 cut(s) 198, 199, 234, 266, 480
BseGI GGATG 2 cut(s) 189, 463
BseLI CCNNNNNNNGG 6 cut(s) 75, 102, 199, 240, 278, 281
BseMII CTCAG 1 cut(s) 528
BseNI ACTGG 2 cut(s) 80, 193
BseRI GAGGAG 2 cut(s) 37, 122
BseSI GKGCMC 1 cut(s) 480
BseXI GCAGC 5 cut(s) 39, 276, 298, 304, 345
Bsh1236I CGCG 3 cut(s) 126, 308, 531
BshFI GGCC 3 cut(s) 244, 271, 323
BshNI GGYRCC 3 cut(s) 224, 310, 475
BshTI ACCGGT 1 cut(s) 495
BsiHKAI GWGCWC 1 cut(s) 132
BsiHKCI CYCGRG 1 cut(s) 199
BsiSI CCGG 3 cut(s) 167, 200, 496
BslFI GGGAC 1 cut(s) 181
BslI CCNNNNNNNGG 6 cut(s) 75, 102, 199, 240, 278, 281
BsmFI GGGAC 1 cut(s) 181
BsnI GGCC 3 cut(s) 244, 271, 323
BsoBI CYCGRG 1 cut(s) 199
Bsp1286I GDGCHC 3 cut(s) 132, 206, 480
Bsp143I GATC 3 cut(s) 60, 115, 532
Bsp1720I GCTNAGC 1 cut(s) 525
Bsp19I CCATGG 1 cut(s) 480
BspACI CCGC 8 cut(s) 164, 222, 251, 275, 308, 314, 378, 450
BspANI GGCC 3 cut(s) 244, 271, 323
BspCNI CTCAG 1 cut(s) 529
BspFNI CGCG 3 cut(s) 126, 308, 531
BspLI GGNNCC 3 cut(s) 226, 312, 477
BspMAI CTGCAG 1 cut(s) 294
BspPI GGATC 1 cut(s) 527
BspQI GCTCTTC 1 cut(s) 267
BspT107I GGYRCC 3 cut(s) 224, 310, 475
BsrFI RCCGGY 1 cut(s) 495
BsrI ACTGG 2 cut(s) 80, 193
BssAI RCCGGY 1 cut(s) 495
BssECI CCNNGG 5 cut(s) 198, 199, 234, 266, 480
BssMI GATC 3 cut(s) 60, 115, 532
BssNI GRCGYC 3 cut(s) 225, 311, 372
BssT1I CCWWGG 1 cut(s) 480
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 1 cut(s) 235
Bst6I CTCTTC 1 cut(s) 267
BstACI GRCGYC 3 cut(s) 225, 311, 372
BstC8I GCNNGC 3 cut(s) 128, 258, 467
BstDEI CTNAG 2 cut(s) 525, 537
BstDSI CCRYGG 3 cut(s) 234, 266, 480
BstF5I GGATG 2 cut(s) 189, 463
BstFNI CGCG 3 cut(s) 126, 308, 531
BstH2I RGCGCY 2 cut(s) 228, 314
BstHHI GCGC 4 cut(s) 126, 227, 313, 531
BstKTI GATC 3 cut(s) 63, 118, 535
BstMBI GATC 3 cut(s) 60, 115, 532
BstMWI GCNNNNNNNGC 7 cut(s) 132, 257, 283, 292, 308, 364, 475
BstNI CCWGG 1 cut(s) 350
BstSCI CCNGG 3 cut(s) 198, 199, 348
BstSFI CTRYAG 1 cut(s) 290
BstSLI GKGCMC 1 cut(s) 480
BstUI CGCG 3 cut(s) 126, 308, 531
BstV1I GCAGC 5 cut(s) 39, 276, 298, 304, 345
BstV2I GAAGAC 1 cut(s) 7
BstXI CCANNNNNNTGG 1 cut(s) 235
BsuRI GGCC 3 cut(s) 244, 271, 323
BtgI CCRYGG 3 cut(s) 234, 266, 480
BtgZI GCGATG 1 cut(s) 419
BtsCI GGATG 2 cut(s) 189, 463
BtsIMutI CAGTG 1 cut(s) 73
Cac8I GCNNGC 3 cut(s) 128, 258, 467
CfoI GCGC 4 cut(s) 126, 227, 313, 531
Cfr10I RCCGGY 1 cut(s) 495
Cfr13I GGNCC 2 cut(s) 346, 493
Cfr9I CCCGGG 1 cut(s) 199
CseI GACGC 2 cut(s) 314, 364
Csp6I GTAC 2 cut(s) 112, 541
CspAI ACCGGT 1 cut(s) 495
CviAII CATG 2 cut(s) 88, 481
CviQI GTAC 2 cut(s) 112, 541
DdeI CTNAG 2 cut(s) 525, 537
DinI GGCGCC 2 cut(s) 226, 312
DpnI GATC 3 cut(s) 62, 117, 534
DpnII GATC 3 cut(s) 60, 115, 532
DrdI GACNNNNNNGTC 1 cut(s) 361
DseDI GACNNNNNNGTC 1 cut(s) 361
EaeI YGGCCR 2 cut(s) 269, 321
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 480
Eco24I GRGCYC 2 cut(s) 132, 206
Eco32I GATATC 1 cut(s) 487
Eco47I GGWCC 2 cut(s) 346, 493
Eco53kI GAGCTC 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 199
EcoICRI GAGCTC 1 cut(s) 130
EcoO109I RGGNCCY 1 cut(s) 346
EcoRII CCWGG 1 cut(s) 348
EcoRV GATATC 1 cut(s) 487
EcoT14I CCWWGG 1 cut(s) 480
EcoT38I GRGCYC 2 cut(s) 132, 206
EgeI GGCGCC 2 cut(s) 226, 312
EheI GGCGCC 2 cut(s) 226, 312
ErhI CCWWGG 1 cut(s) 480
FaeI CATG 2 cut(s) 91, 484
FaiI YATR 4 cut(s) 89, 137, 437, 482
FaqI GGGAC 1 cut(s) 181
FatI CATG 2 cut(s) 87, 480
FauI CCCGC 1 cut(s) 171
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 9 cut(s) 28, 223, 287, 290, 293, 309, 314, 359, 451
FokI GGATG 2 cut(s) 176, 470
FriOI GRGCYC 2 cut(s) 132, 206
Fsp4HI GCNGC 9 cut(s) 28, 223, 287, 290, 293, 309, 314, 359, 451
FspBI CTAG 2 cut(s) 179, 240
GlaI GCGC 4 cut(s) 125, 226, 312, 530
GluI GCNGC 9 cut(s) 28, 223, 287, 290, 293, 309, 314, 359, 451
HaeII RGCGCY 2 cut(s) 228, 314
HaeIII GGCC 3 cut(s) 244, 271, 323
HapII CCGG 3 cut(s) 167, 200, 496
HgaI GACGC 2 cut(s) 314, 364
HhaI GCGC 4 cut(s) 126, 227, 313, 531
Hin1I GRCGYC 3 cut(s) 225, 311, 372
Hin1II CATG 2 cut(s) 91, 484
Hin6I GCGC 4 cut(s) 124, 225, 311, 529
HinP1I GCGC 4 cut(s) 124, 225, 311, 529
HinfI GANTC 5 cut(s) 9, 22, 79, 390, 421
HpaII CCGG 3 cut(s) 167, 200, 496
HphI GGTGA 2 cut(s) 223, 367
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 1 cut(s) 8
Hpy188III TCNNGA 6 cut(s) 19, 83, 394, 505, 515, 536
Hpy8I GTNNAC 1 cut(s) 187
Hpy99I CGWCG 5 cut(s) 104, 250, 308, 358, 367
HpyAV CCTTC 4 cut(s) 61, 79, 106, 220
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 3 cut(s) 135, 292, 439
HpyF10VI GCNNNNNNNGC 7 cut(s) 132, 257, 283, 292, 308, 364, 475
HpyF3I CTNAG 2 cut(s) 525, 537
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 3 cut(s) 225, 311, 372
Hsp92II CATG 2 cut(s) 91, 484
HspAI GCGC 4 cut(s) 124, 225, 311, 529
KasI GGCGCC 2 cut(s) 224, 310
Kzo9I GATC 3 cut(s) 60, 115, 532
LguI GCTCTTC 1 cut(s) 267
LmnI GCTCC 1 cut(s) 462
Lsp1109I GCAGC 5 cut(s) 39, 276, 298, 304, 345
MaeI CTAG 2 cut(s) 179, 240
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 1 cut(s) 373
MalI GATC 3 cut(s) 62, 117, 534
MboI GATC 3 cut(s) 60, 115, 532
MboII GAAGA 4 cut(s) 7, 205, 208, 254
MhlI GDGCHC 3 cut(s) 132, 206, 480
MlsI TGGCCA 1 cut(s) 271
MluCI AATT 1 cut(s) 430
MluNI TGGCCA 1 cut(s) 271
Mly113I GGCGCC 2 cut(s) 225, 311
MlyI GAGTC 1 cut(s) 18
MnlI CCTC 5 cut(s) 58, 100, 337, 390, 513
Mox20I TGGCCA 1 cut(s) 271
MroXI GAANNNNTTC 3 cut(s) 95, 425, 551
MscI TGGCCA 1 cut(s) 271
MseI TTAA 1 cut(s) 146
MslI CAYNNNNRTG 1 cut(s) 233
Msp20I TGGCCA 1 cut(s) 271
MspA1I CMGCKG 2 cut(s) 289, 316
MspI CCGG 3 cut(s) 167, 200, 496
MspR9I CCNGG 3 cut(s) 200, 201, 350
MvaI CCWGG 1 cut(s) 350
MvnI CGCG 3 cut(s) 126, 308, 531
MwoI GCNNNNNNNGC 7 cut(s) 132, 257, 283, 292, 308, 364, 475
NarI GGCGCC 2 cut(s) 225, 311
NciI CCSGG 2 cut(s) 200, 201
NcoI CCATGG 1 cut(s) 480
NdeII GATC 3 cut(s) 60, 115, 532
NlaIII CATG 2 cut(s) 91, 484
NlaIV GGNNCC 3 cut(s) 226, 312, 477
NmeAIII GCCGAG 1 cut(s) 406
NmuCI GTSAC 1 cut(s) 373
PciSI GCTCTTC 1 cut(s) 267
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PdmI GAANNNNTTC 3 cut(s) 95, 425, 551
PfeI GAWTC 4 cut(s) 22, 79, 390, 421
PinAI ACCGGT 1 cut(s) 495
PkrI GCNGC 9 cut(s) 29, 224, 288, 291, 294, 310, 315, 360, 452
PleI GAGTC 1 cut(s) 17
PluTI GGCGCC 2 cut(s) 228, 314
PpsI GAGTC 1 cut(s) 17
PpuMI RGGWCCY 1 cut(s) 346
Psp124BI GAGCTC 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 346
Psp6I CCWGG 1 cut(s) 348
PspGI CCWGG 1 cut(s) 348
PspN4I GGNNCC 3 cut(s) 226, 312, 477
PspPI GGNCC 2 cut(s) 346, 493
PspPPI RGGWCCY 1 cut(s) 346
PstI CTGCAG 1 cut(s) 294
PvuII CAGCTG 1 cut(s) 289
RsaI GTAC 2 cut(s) 113, 542
RsaNI GTAC 2 cut(s) 112, 541
RseI CAYNNNNRTG 1 cut(s) 233
SacI GAGCTC 1 cut(s) 132
SapI GCTCTTC 1 cut(s) 267
SaqAI TTAA 1 cut(s) 146
SatI GCNGC 9 cut(s) 28, 223, 287, 290, 293, 309, 314, 359, 451
Sau3AI GATC 3 cut(s) 60, 115, 532
Sau96I GGNCC 2 cut(s) 346, 493
ScaI AGTACT 1 cut(s) 542
SchI GAGTC 1 cut(s) 18
ScrFI CCNGG 3 cut(s) 200, 201, 350
SduI GDGCHC 3 cut(s) 132, 206, 480
SfcI CTRYAG 1 cut(s) 290
SfoI GGCGCC 2 cut(s) 226, 312
SinI GGWCC 2 cut(s) 346, 493
SmaI CCCGGG 1 cut(s) 201
SmiMI CAYNNNNRTG 1 cut(s) 233
Sse9I AATT 1 cut(s) 430
SsiI CCGC 8 cut(s) 164, 222, 251, 275, 308, 314, 378, 450
SspDI GGCGCC 2 cut(s) 224, 310
SspMI CTAG 2 cut(s) 179, 240
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 3 cut(s) 198, 199, 348
StyI CCWWGG 1 cut(s) 480
TaaI ACNGT 1 cut(s) 235
TaiI ACGT 1 cut(s) 375
TaqI TCGA 3 cut(s) 63, 84, 393
TasI AATT 1 cut(s) 430
TatI WGTACW 1 cut(s) 540
TauI GCSGC 4 cut(s) 225, 311, 316, 453
TfiI GAWTC 4 cut(s) 22, 79, 390, 421
Tru1I TTAA 1 cut(s) 146
Tru9I TTAA 1 cut(s) 146
TscAI CASTG 1 cut(s) 80
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 5 cut(s) 27, 286, 289, 292, 358
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 2 cut(s) 104, 422
TspGWI ACGGA 1 cut(s) 255
TspMI CCCGGG 1 cut(s) 199
TspRI CASTG 1 cut(s) 80
VpaK11BI GGWCC 2 cut(s) 346, 493
XcmI CCANNNNNNNNNTGG 1 cut(s) 487
XmaI CCCGGG 1 cut(s) 199
XmiI GTMKAC 1 cut(s) 186
XmnI GAANNNNTTC 3 cut(s) 95, 425, 551
XspI CTAG 2 cut(s) 179, 240
ZraI GACGTC 1 cut(s) 373
ZrmI AGTACT 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.