MD09G1265200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014266
Physical Location & Seq
Forward (+)
33940808 .. 33943580
2773 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1265200.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGCGGAGCCGTACGGAACCAACGGCGGAGTCGACGTCGACGTTACCGACAACGACGGCGAGATCCACACCAACCGGGAGGAGACTCTCTACGGCATCTTCCACCATCTGATTGTTGAGATCCTCTTCCCCGGCTCCACCAGTGCCGGTGGTGCTGCTCCGCTGTTTCAGCGGATCAAAACCTCTCTAGCCAAGAACGGCCCGCTGCTTCACGAAGCTTCCAGAAACAGTGGGCGAAACGTTTTTCTCTGGACTCGGAGGGGGAGCCCTCTCCGTGCACTGCTAGTCATCTCTGTTGGCACGATTACTCTTCTTGCCTTGACAGGGTTGCTTGTCTTCATGCTTTTCTTTGTGGCAGCAACTTTCAATGCCGTTGTTATCTCTCTCCTACTGTCTCTGGCAGCTGCAGGAGGATTCTTGGCCATTTTCTTTGCCTGTGTAACAGCGATATACATAGGAGCGTTATCAGTGGCTGTTTTTGTTATTTCCGCCACAACAATATTGACAATTGGAGCTGTTCTGATAGCTACAGGTTGGATTGGATTCTTCTGGATCATGTGGCTGGCAACAAAGAAATCTCTGGGCCTTGCTAAGCAATCGTTGAATGTGACTGGTTCGGCGATTTCAGCGTACTCTTATGGCAGGCATGCTCGCTATCATTCAGCGATAGGTAAAGCTTCCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

229

Amino Acids

24.18

Weight (kDa)

9.37

Isoelectric Point (pI)

22.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014266)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16550
fragaria_vesca FvH4_1g28640
malus_domestica MD09G1265200.v1.1 MD17G1261400.v1.1
prunus_persica Prupe.3G163400_v2.0.a1
pyrus_communis pycom09g17900 pycom17g26190
rosa_chinensis RchiOBHm_Chr3g0492651
rosa_laevigata RLG00000022752
rosa_multiflora Rmu_sc0000831.1_g000016
rosa_roxburghii Rroxscaffold_6G00392160
rosa_rugosa Rorug03G0255300.1
rosa_samantha Rh3AG305600 Rh3BG342000 Rh3CG339400 Rh3DG341300
rosa_wichuraiana Rw3G026900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 39
AccI GTMKAC 2 cut(s) 33, 39
AciI CCGC 6 cut(s) 5, 27, 161, 172, 203, 489
AclI AACGTT 1 cut(s) 240
AclWI GGATC 4 cut(s) 58, 115, 182, 560
AcoI YGGCCR 1 cut(s) 420
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 2 cut(s) 14, 632
AfiI CCNNNNNNNGG 1 cut(s) 324
AgsI TTSAA 2 cut(s) 367, 604
AluBI AGCT 5 cut(s) 218, 404, 515, 527, 677
AluI AGCT 5 cut(s) 218, 404, 515, 527, 677
Alw21I GWGCWC 1 cut(s) 280
Alw26I GTCTC 2 cut(s) 77, 399
Alw44I GTGCAC 1 cut(s) 276
AlwI GGATC 4 cut(s) 58, 115, 182, 560
AlwNI CAGNNNCTG 1 cut(s) 473
AoxI GGCC 3 cut(s) 199, 420, 583
ApaLI GTGCAC 1 cut(s) 276
ApeKI GCWGC 5 cut(s) 155, 205, 356, 401, 404
AspS9I GGNCC 2 cut(s) 200, 583
AsuC2I CCSGG 2 cut(s) 77, 132
BaeGI GKGCMC 1 cut(s) 280
BalI TGGCCA 1 cut(s) 422
BanII GRGCYC 1 cut(s) 269
BbsI GAAGAC 1 cut(s) 328
Bbv12I GWGCWC 1 cut(s) 280
BbvI GCAGC 5 cut(s) 142, 192, 368, 391, 413
BccI CCATC 1 cut(s) 114
BceAI ACGGC 5 cut(s) 40, 73, 109, 214, 356
BcnI CCSGG 2 cut(s) 77, 132
BcoDI GTCTC 2 cut(s) 77, 399
BfaI CTAG 2 cut(s) 188, 284
BfmI CTRYAG 2 cut(s) 405, 528
BisI GCNGC 5 cut(s) 156, 206, 357, 402, 405
BlpI GCTNAGC 1 cut(s) 591
BlsI GCNGC 5 cut(s) 157, 207, 358, 403, 406
Bme1390I CCNGG 2 cut(s) 77, 132
BmgT120I GGNCC 2 cut(s) 200, 583
BmiI GGNNCC 4 cut(s) 9, 19, 136, 266
BmrFI CCNGG 2 cut(s) 77, 132
BmsI GCATC 1 cut(s) 105
BpiI GAAGAC 1 cut(s) 328
Bpu1102I GCTNAGC 1 cut(s) 591
BpuMI CCSGG 2 cut(s) 77, 132
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 130
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 1 cut(s) 324
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 2 cut(s) 141, 616
BseDI CCNNGG 1 cut(s) 130
BseLI CCNNNNNNNGG 1 cut(s) 324
BseNI ACTGG 2 cut(s) 141, 616
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 1 cut(s) 280
BseXI GCAGC 5 cut(s) 142, 192, 368, 391, 413
BshFI GGCC 3 cut(s) 201, 422, 585
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 3 cut(s) 76, 132, 147
BsiWI CGTACG 1 cut(s) 12
BslI CCNNNNNNNGG 1 cut(s) 324
BsmAI GTCTC 2 cut(s) 77, 399
BsnI GGCC 3 cut(s) 201, 422, 585
Bsp1286I GDGCHC 2 cut(s) 269, 280
Bsp143I GATC 4 cut(s) 63, 120, 174, 552
Bsp1720I GCTNAGC 1 cut(s) 591
BspACI CCGC 6 cut(s) 5, 27, 161, 172, 203, 489
BspANI GGCC 3 cut(s) 201, 422, 585
BspLI GGNNCC 4 cut(s) 9, 19, 136, 266
BspMAI CTGCAG 1 cut(s) 409
BspPI GGATC 4 cut(s) 58, 115, 182, 560
BsrFI RCCGGY 1 cut(s) 146
BsrI ACTGG 2 cut(s) 141, 616
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 1 cut(s) 130
BssMI GATC 4 cut(s) 63, 120, 174, 552
BssNI GRCGYC 1 cut(s) 36
Bst4CI ACNGT 2 cut(s) 230, 393
Bst6I CTCTTC 2 cut(s) 131, 315
BstACI GRCGYC 1 cut(s) 36
BstC8I GCNNGC 5 cut(s) 203, 564, 644, 648, 652
BstDEI CTNAG 1 cut(s) 591
BstKTI GATC 4 cut(s) 66, 123, 177, 555
BstMAI GTCTC 2 cut(s) 77, 399
BstMBI GATC 4 cut(s) 63, 120, 174, 552
BstMWI GCNNNNNNNGC 3 cut(s) 152, 169, 626
BstNSI RCATGY 1 cut(s) 650
BstSCI CCNGG 2 cut(s) 75, 130
BstSFI CTRYAG 2 cut(s) 405, 528
BstSLI GKGCMC 1 cut(s) 280
BstV1I GCAGC 5 cut(s) 142, 192, 368, 391, 413
BstV2I GAAGAC 1 cut(s) 328
BstX2I RGATCY 2 cut(s) 63, 120
BstYI RGATCY 2 cut(s) 63, 120
BsuRI GGCC 3 cut(s) 201, 422, 585
BtsI GCAGTG 1 cut(s) 278
BtsIMutI CAGTG 4 cut(s) 148, 235, 278, 474
Cac8I GCNNGC 5 cut(s) 203, 564, 644, 648, 652
CaiI CAGNNNCTG 1 cut(s) 473
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 2 cut(s) 200, 583
Csp6I GTAC 2 cut(s) 13, 631
CviAII CATG 3 cut(s) 340, 556, 647
CviQI GTAC 2 cut(s) 13, 631
DdeI CTNAG 1 cut(s) 591
DpnI GATC 4 cut(s) 65, 122, 176, 554
DpnII GATC 4 cut(s) 63, 120, 174, 552
EaeI YGGCCR 1 cut(s) 420
Eam1104I CTCTTC 2 cut(s) 131, 315
EarI CTCTTC 2 cut(s) 131, 315
EciI GGCGGA 3 cut(s) 20, 42, 478
Eco24I GRGCYC 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 269
FaeI CATG 3 cut(s) 343, 559, 650
FaiI YATR 6 cut(s) 341, 451, 455, 557, 639, 648
FatI CATG 3 cut(s) 339, 555, 646
FauI CCCGC 1 cut(s) 210
FblI GTMKAC 2 cut(s) 33, 39
Fnu4HI GCNGC 5 cut(s) 156, 206, 357, 402, 405
FriOI GRGCYC 1 cut(s) 269
Fsp4HI GCNGC 5 cut(s) 156, 206, 357, 402, 405
FspBI CTAG 2 cut(s) 188, 284
GluI GCNGC 5 cut(s) 156, 206, 357, 402, 405
HaeIII GGCC 3 cut(s) 201, 422, 585
HapII CCGG 3 cut(s) 76, 132, 147
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 3 cut(s) 343, 559, 650
HincII GTYRAC 2 cut(s) 34, 40
HindII GTYRAC 2 cut(s) 34, 40
HindIII AAGCTT 2 cut(s) 216, 675
HinfI GANTC 5 cut(s) 30, 85, 253, 414, 543
HpaII CCGG 3 cut(s) 76, 132, 147
Hpy166II GTNNAC 3 cut(s) 34, 40, 278
Hpy188I TCNGA 4 cut(s) 111, 258, 522, 682
Hpy188III TCNNGA 4 cut(s) 212, 222, 250, 550
Hpy8I GTNNAC 3 cut(s) 34, 40, 278
Hpy99I CGWCG 4 cut(s) 38, 41, 44, 59
HpyCH4III ACNGT 2 cut(s) 230, 393
HpyCH4IV ACGT 3 cut(s) 36, 42, 240
HpyCH4V TGCA 2 cut(s) 278, 407
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 169, 626
HpyF3I CTNAG 1 cut(s) 591
HpySE526I ACGT 3 cut(s) 36, 42, 240
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 3 cut(s) 343, 559, 650
Kzo9I GATC 4 cut(s) 63, 120, 174, 552
LmnI GCTCC 6 cut(s) 7, 140, 163, 264, 458, 512
Lsp1109I GCAGC 5 cut(s) 142, 192, 368, 391, 413
LweI GCATC 1 cut(s) 105
MaeI CTAG 2 cut(s) 188, 284
MaeII ACGT 3 cut(s) 36, 42, 240
MaeIII GTNAC 3 cut(s) 43, 439, 607
MalI GATC 4 cut(s) 65, 122, 176, 554
MboI GATC 4 cut(s) 63, 120, 174, 552
MboII GAAGA 5 cut(s) 91, 118, 302, 328, 538
MfeI CAATTG 1 cut(s) 507
MflI RGATCY 2 cut(s) 63, 120
MhlI GDGCHC 2 cut(s) 269, 280
MlsI TGGCCA 1 cut(s) 422
MluCI AATT 1 cut(s) 507
MluNI TGGCCA 1 cut(s) 422
MlyI GAGTC 3 cut(s) 39, 79, 247
MmeI TCCRAC 1 cut(s) 515
MnlI CCTC 6 cut(s) 73, 134, 193, 252, 279, 404
Mox20I TGGCCA 1 cut(s) 422
MscI TGGCCA 1 cut(s) 422
Msp20I TGGCCA 1 cut(s) 422
MspA1I CMGCKG 4 cut(s) 163, 172, 205, 404
MspI CCGG 3 cut(s) 76, 132, 147
MspR9I CCNGG 2 cut(s) 77, 132
MunI CAATTG 1 cut(s) 507
MwoI GCNNNNNNNGC 3 cut(s) 152, 169, 626
NciI CCSGG 2 cut(s) 77, 132
NdeII GATC 4 cut(s) 63, 120, 174, 552
NlaIII CATG 3 cut(s) 343, 559, 650
NlaIV GGNNCC 4 cut(s) 9, 19, 136, 266
NmuCI GTSAC 1 cut(s) 607
NspI RCATGY 1 cut(s) 650
PaeI GCATGC 1 cut(s) 650
PcsI WCGNNNNNNNCGW 3 cut(s) 30, 39, 45
PfeI GAWTC 2 cut(s) 414, 543
Pfl23II CGTACG 1 cut(s) 12
PkrI GCNGC 5 cut(s) 157, 207, 358, 403, 406
PleI GAGTC 3 cut(s) 38, 79, 247
PpsI GAGTC 3 cut(s) 38, 79, 247
Psp1406I AACGTT 1 cut(s) 240
PspLI CGTACG 1 cut(s) 12
PspN4I GGNNCC 4 cut(s) 9, 19, 136, 266
PspPI GGNCC 2 cut(s) 200, 583
PstI CTGCAG 1 cut(s) 409
PstNI CAGNNNCTG 1 cut(s) 473
PsuI RGATCY 2 cut(s) 63, 120
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 2 cut(s) 14, 632
RsaNI GTAC 2 cut(s) 13, 631
SalI GTCGAC 2 cut(s) 32, 38
SatI GCNGC 5 cut(s) 156, 206, 357, 402, 405
Sau3AI GATC 4 cut(s) 63, 120, 174, 552
Sau96I GGNCC 2 cut(s) 200, 583
SchI GAGTC 3 cut(s) 39, 79, 247
ScrFI CCNGG 2 cut(s) 77, 132
SduI GDGCHC 2 cut(s) 269, 280
SfaNI GCATC 1 cut(s) 105
SfcI CTRYAG 2 cut(s) 405, 528
SgrDI CGTCGACG 1 cut(s) 38
SphI GCATGC 1 cut(s) 650
Sse9I AATT 1 cut(s) 507
SsiI CCGC 6 cut(s) 5, 27, 161, 172, 203, 489
SspI AATATT 1 cut(s) 501
SspMI CTAG 2 cut(s) 188, 284
StyD4I CCNGG 2 cut(s) 75, 130
TaaI ACNGT 2 cut(s) 230, 393
TaiI ACGT 3 cut(s) 39, 45, 243
TaqI TCGA 2 cut(s) 33, 39
TasI AATT 1 cut(s) 507
TfiI GAWTC 2 cut(s) 414, 543
TscAI CASTG 4 cut(s) 148, 235, 285, 474
TseFI GTSAC 1 cut(s) 607
TseI GCWGC 5 cut(s) 155, 205, 356, 401, 404
Tsp45I GTSAC 1 cut(s) 607
TspDTI ATGAA 1 cut(s) 328
TspGWI ACGGA 2 cut(s) 30, 263
TspRI CASTG 4 cut(s) 148, 235, 285, 474
VneI GTGCAC 1 cut(s) 276
XceI RCATGY 1 cut(s) 650
XmiI GTMKAC 2 cut(s) 33, 39
XspI CTAG 2 cut(s) 188, 284
ZraI GACGTC 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.