MD17G1261400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014266
Physical Location & Seq
Forward (+)
32220026 .. 32224392
4367 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1261400.v1.1.491

Sequence Viewer

Length: 684 bp
ATGGCGGAGCCGTACGGAACCAACGGCGGAGTCCACGTGGACGTCATCGACCACGACGGCGAGGTCCACACCAAACGGGAGGAGACTCTCTACGGCGTTTTCCACCGTCTGATCATTGAGATCTTCTTCCCCGGCTCCACCAGTGCCGGCGCTACTGCTCCGCTGTTTCACCGGATCAAAACCTCCCTAGCCAAGAACGTTCCGCTGCTTCGCGAAGCTTCTAGAAACAGTGGGCGGAACGTTCTTCTGTGGACTCGGAGGGGGAGTCCTCTCCGTGCACTCCTGGTTATCTCTGTTGGCACAATTACTCTTGTTGCCTTGACAGGATTGCTTGTCTTCATGCTTTTCTTAATGGCAGCAACTTTCAATGCCGTTGTTATTTCTCTCCTACTGTCTTTGGCAGCTGCCGGAGGGTTCTTGGCCATTTTCTTTGCCTGTGTAACAGTTATATACATTGGAGCATTATCAGTGGCTGTTTTTGTTATTTCTGCCACAACAATAACTGCAATGGGAGCTGTTCTGTTTACTACAGGTTGGATTGGATTCTTTTGGATCGTGTTGCTGGCAACAAAGAAATCTCTGGGCCTTGCTAAGAAGTCATTTGGTGCGACTGGTTCAGCGCTTGCAGCTTACTCTTATACTAGGCATGCTCGCCATCATTCAGCAATCGATAAGGCAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

228

Amino Acids

24.24

Weight (kDa)

9.93

Isoelectric Point (pI)

24.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014266)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16550
fragaria_vesca FvH4_1g28640
malus_domestica MD09G1265200.v1.1 MD17G1261400.v1.1
prunus_persica Prupe.3G163400_v2.0.a1
pyrus_communis pycom09g17900 pycom17g26190
rosa_chinensis RchiOBHm_Chr3g0492651
rosa_laevigata RLG00000022752
rosa_multiflora Rmu_sc0000831.1_g000016
rosa_roxburghii Rroxscaffold_6G00392160
rosa_rugosa Rorug03G0255300.1
rosa_samantha Rh3AG305600 Rh3BG342000 Rh3CG339400 Rh3DG341300
rosa_wichuraiana Rw3G026900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 62
AatII GACGTC 1 cut(s) 45
AccII CGCG 1 cut(s) 213
AciI CCGC 5 cut(s) 5, 27, 161, 203, 235
AclI AACGTT 2 cut(s) 198, 240
AclWI GGATC 2 cut(s) 182, 560
AcoI YGGCCR 1 cut(s) 420
AcvI CACGTG 1 cut(s) 37
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 1 cut(s) 14
AfeI AGCGCT 1 cut(s) 621
AgsI TTSAA 1 cut(s) 367
AjnI CCWGG 1 cut(s) 282
AluBI AGCT 4 cut(s) 218, 404, 515, 629
AluI AGCT 4 cut(s) 218, 404, 515, 629
Alw21I GWGCWC 1 cut(s) 280
Alw26I GTCTC 1 cut(s) 77
Alw44I GTGCAC 1 cut(s) 276
AlwI GGATC 2 cut(s) 182, 560
AlwNI CAGNNNCTG 1 cut(s) 473
Aor51HI AGCGCT 1 cut(s) 621
AoxI GGCC 2 cut(s) 420, 583
ApaLI GTGCAC 1 cut(s) 276
ApeKI GCWGC 5 cut(s) 205, 356, 401, 404, 626
AspLEI GCGC 2 cut(s) 152, 622
AspS9I GGNCC 2 cut(s) 64, 583
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 1 cut(s) 161
AvaII GGWCC 1 cut(s) 64
BaeGI GKGCMC 1 cut(s) 280
BalI TGGCCA 1 cut(s) 422
BbrPI CACGTG 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 328
Bbv12I GWGCWC 1 cut(s) 280
BbvI GCAGC 5 cut(s) 192, 368, 391, 413, 638
BccI CCATC 1 cut(s) 663
BceAI ACGGC 4 cut(s) 40, 73, 109, 356
BciT130I CCWGG 1 cut(s) 284
BclI TGATCA 1 cut(s) 111
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 1 cut(s) 77
BfaI CTAG 3 cut(s) 188, 222, 642
BfmI CTRYAG 1 cut(s) 528
BfoI RGCGCY 2 cut(s) 153, 623
BglII AGATCT 1 cut(s) 120
BisI GCNGC 5 cut(s) 206, 357, 402, 405, 627
BlsI GCNGC 5 cut(s) 207, 358, 403, 406, 628
Bme1390I CCNGG 2 cut(s) 132, 284
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 2 cut(s) 64, 583
BmiI GGNNCC 3 cut(s) 9, 19, 136
BmrFI CCNGG 2 cut(s) 132, 284
BpiI GAAGAC 1 cut(s) 328
BpuMI CCSGG 1 cut(s) 132
Bsa29I ATCGAT 1 cut(s) 669
BsaAI YACGTR 1 cut(s) 37
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 1 cut(s) 130
BsaWI WCCGGW 1 cut(s) 171
BsaXI ACNNNNNCTCC 4 cut(s) 21, 51, 250, 280
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 2 cut(s) 141, 616
Bse3DI GCAATG 1 cut(s) 513
BseBI CCWGG 1 cut(s) 284
BseCI ATCGAT 1 cut(s) 669
BseDI CCNNGG 1 cut(s) 130
BseMI GCAATG 1 cut(s) 513
BseNI ACTGG 2 cut(s) 141, 616
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 1 cut(s) 280
BseXI GCAGC 5 cut(s) 192, 368, 391, 413, 638
Bsh1236I CGCG 1 cut(s) 213
BshFI GGCC 2 cut(s) 422, 585
BshVI ATCGAT 1 cut(s) 669
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 4 cut(s) 132, 147, 172, 408
BsiWI CGTACG 1 cut(s) 12
BsmAI GTCTC 1 cut(s) 77
BsnI GGCC 2 cut(s) 422, 585
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 4 cut(s) 111, 120, 174, 552
Bsp68I TCGCGA 1 cut(s) 213
BspACI CCGC 5 cut(s) 5, 27, 161, 203, 235
BspANI GGCC 2 cut(s) 422, 585
BspDI ATCGAT 1 cut(s) 669
BspFNI CGCG 1 cut(s) 213
BspLI GGNNCC 3 cut(s) 9, 19, 136
BspPI GGATC 2 cut(s) 182, 560
BsrDI GCAATG 1 cut(s) 513
BsrFI RCCGGY 1 cut(s) 146
BsrI ACTGG 2 cut(s) 141, 616
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 1 cut(s) 130
BssMI GATC 4 cut(s) 111, 120, 174, 552
BssNI GRCGYC 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 284
Bst4CI ACNGT 4 cut(s) 107, 230, 393, 445
BstACI GRCGYC 1 cut(s) 42
BstBAI YACGTR 1 cut(s) 37
BstC8I GCNNGC 5 cut(s) 148, 564, 624, 648, 652
BstDEI CTNAG 1 cut(s) 591
BstFNI CGCG 1 cut(s) 213
BstH2I RGCGCY 2 cut(s) 153, 623
BstHHI GCGC 2 cut(s) 152, 622
BstKTI GATC 4 cut(s) 114, 123, 177, 555
BstMAI GTCTC 1 cut(s) 77
BstMBI GATC 4 cut(s) 111, 120, 174, 552
BstMWI GCNNNNNNNGC 2 cut(s) 512, 626
BstNI CCWGG 1 cut(s) 284
BstNSI RCATGY 1 cut(s) 650
BstSCI CCNGG 2 cut(s) 130, 282
BstSFI CTRYAG 1 cut(s) 528
BstSLI GKGCMC 1 cut(s) 280
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 5 cut(s) 192, 368, 391, 413, 638
BstV2I GAAGAC 1 cut(s) 328
BstX2I RGATCY 1 cut(s) 120
BstYI RGATCY 1 cut(s) 120
Bsu15I ATCGAT 1 cut(s) 669
BsuRI GGCC 2 cut(s) 422, 585
BsuTUI ATCGAT 1 cut(s) 669
BtsIMutI CAGTG 3 cut(s) 148, 235, 474
BtuMI TCGCGA 1 cut(s) 213
Cac8I GCNNGC 5 cut(s) 148, 564, 624, 648, 652
CaiI CAGNNNCTG 1 cut(s) 473
CfoI GCGC 2 cut(s) 152, 622
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 2 cut(s) 64, 583
ClaI ATCGAT 1 cut(s) 669
Csp6I GTAC 1 cut(s) 13
CviAII CATG 2 cut(s) 340, 647
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 1 cut(s) 591
DpnI GATC 4 cut(s) 113, 122, 176, 554
DpnII GATC 4 cut(s) 111, 120, 174, 552
DrdI GACNNNNNNGTC 1 cut(s) 62
DseDI GACNNNNNNGTC 1 cut(s) 62
EaeI YGGCCR 1 cut(s) 420
EciI GGCGGA 3 cut(s) 20, 42, 250
Eco47I GGWCC 1 cut(s) 64
Eco47III AGCGCT 1 cut(s) 621
Eco72I CACGTG 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 282
FaeI CATG 2 cut(s) 343, 650
FaiI YATR 5 cut(s) 341, 449, 451, 639, 648
FatI CATG 2 cut(s) 339, 646
FbaI TGATCA 1 cut(s) 111
Fnu4HI GCNGC 5 cut(s) 206, 357, 402, 405, 627
Fsp4HI GCNGC 5 cut(s) 206, 357, 402, 405, 627
FspBI CTAG 3 cut(s) 188, 222, 642
GlaI GCGC 2 cut(s) 151, 621
GluI GCNGC 5 cut(s) 206, 357, 402, 405, 627
HaeII RGCGCY 2 cut(s) 153, 623
HaeIII GGCC 2 cut(s) 422, 585
HapII CCGG 4 cut(s) 132, 147, 172, 408
HhaI GCGC 2 cut(s) 152, 622
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 2 cut(s) 343, 650
Hin6I GCGC 2 cut(s) 150, 620
HinP1I GCGC 2 cut(s) 150, 620
HindIII AAGCTT 1 cut(s) 216
HinfI GANTC 5 cut(s) 30, 85, 253, 265, 543
HpaII CCGG 4 cut(s) 132, 147, 172, 408
HphI GGTGA 1 cut(s) 161
Hpy166II GTNNAC 6 cut(s) 34, 40, 67, 252, 278, 525
Hpy188I TCNGA 2 cut(s) 111, 258
Hpy188III TCNNGA 2 cut(s) 212, 222
Hpy8I GTNNAC 6 cut(s) 34, 40, 67, 252, 278, 525
Hpy99I CGWCG 1 cut(s) 59
HpyCH4III ACNGT 4 cut(s) 107, 230, 393, 445
HpyCH4IV ACGT 4 cut(s) 36, 42, 198, 240
HpyCH4V TGCA 3 cut(s) 278, 506, 626
HpyF10VI GCNNNNNNNGC 2 cut(s) 512, 626
HpyF3I CTNAG 1 cut(s) 591
HpySE526I ACGT 4 cut(s) 36, 42, 198, 240
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 2 cut(s) 343, 650
HspAI GCGC 2 cut(s) 150, 620
KroI GCCGGC 1 cut(s) 146
KroNI GCCGGC 1 cut(s) 148
Ksp22I TGATCA 1 cut(s) 111
Kzo9I GATC 4 cut(s) 111, 120, 174, 552
LmnI GCTCC 5 cut(s) 7, 140, 163, 458, 512
Lsp1109I GCAGC 5 cut(s) 192, 368, 391, 413, 638
MaeI CTAG 3 cut(s) 188, 222, 642
MaeII ACGT 4 cut(s) 36, 42, 198, 240
MaeIII GTNAC 1 cut(s) 439
MalI GATC 4 cut(s) 113, 122, 176, 554
MboI GATC 4 cut(s) 111, 120, 174, 552
MboII GAAGA 4 cut(s) 115, 118, 236, 328
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 1 cut(s) 280
MlsI TGGCCA 1 cut(s) 422
MluCI AATT 1 cut(s) 303
MluNI TGGCCA 1 cut(s) 422
MlyI GAGTC 4 cut(s) 39, 79, 247, 274
MmeI TCCRAC 1 cut(s) 515
MnlI CCTC 6 cut(s) 55, 73, 193, 252, 279, 404
Mox20I TGGCCA 1 cut(s) 422
MroNI GCCGGC 1 cut(s) 146
MscI TGGCCA 1 cut(s) 422
MseI TTAA 1 cut(s) 350
Msp20I TGGCCA 1 cut(s) 422
MspA1I CMGCKG 3 cut(s) 163, 205, 404
MspI CCGG 4 cut(s) 132, 147, 172, 408
MspR9I CCNGG 2 cut(s) 132, 284
MvaI CCWGG 1 cut(s) 284
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 2 cut(s) 512, 626
NaeI GCCGGC 1 cut(s) 148
NciI CCSGG 1 cut(s) 132
NdeII GATC 4 cut(s) 111, 120, 174, 552
NgoMIV GCCGGC 1 cut(s) 146
NlaIII CATG 2 cut(s) 343, 650
NlaIV GGNNCC 3 cut(s) 9, 19, 136
NruI TCGCGA 1 cut(s) 213
NspI RCATGY 1 cut(s) 650
PaeI GCATGC 1 cut(s) 650
PdiI GCCGGC 1 cut(s) 148
PfeI GAWTC 1 cut(s) 543
Pfl23II CGTACG 1 cut(s) 12
PkrI GCNGC 5 cut(s) 207, 358, 403, 406, 628
PleI GAGTC 4 cut(s) 38, 79, 247, 273
PmaCI CACGTG 1 cut(s) 37
PmlI CACGTG 1 cut(s) 37
PpsI GAGTC 4 cut(s) 38, 79, 247, 273
Ppu21I YACGTR 1 cut(s) 37
Psp1406I AACGTT 2 cut(s) 198, 240
Psp6I CCWGG 1 cut(s) 282
PspCI CACGTG 1 cut(s) 37
PspGI CCWGG 1 cut(s) 282
PspLI CGTACG 1 cut(s) 12
PspN4I GGNNCC 3 cut(s) 9, 19, 136
PspPI GGNCC 2 cut(s) 64, 583
PstNI CAGNNNCTG 1 cut(s) 473
PsuI RGATCY 1 cut(s) 120
PvuII CAGCTG 1 cut(s) 404
RruI TCGCGA 1 cut(s) 213
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
SaqAI TTAA 1 cut(s) 350
SatI GCNGC 5 cut(s) 206, 357, 402, 405, 627
Sau3AI GATC 4 cut(s) 111, 120, 174, 552
Sau96I GGNCC 2 cut(s) 64, 583
SchI GAGTC 4 cut(s) 39, 79, 247, 274
ScrFI CCNGG 2 cut(s) 132, 284
SduI GDGCHC 1 cut(s) 280
SfcI CTRYAG 1 cut(s) 528
SinI GGWCC 1 cut(s) 64
SphI GCATGC 1 cut(s) 650
Sse9I AATT 1 cut(s) 303
SsiI CCGC 5 cut(s) 5, 27, 161, 203, 235
SspMI CTAG 3 cut(s) 188, 222, 642
StyD4I CCNGG 2 cut(s) 130, 282
TaaI ACNGT 4 cut(s) 107, 230, 393, 445
TaiI ACGT 4 cut(s) 39, 45, 201, 243
TaqI TCGA 2 cut(s) 48, 669
TasI AATT 1 cut(s) 303
TfiI GAWTC 1 cut(s) 543
Tru1I TTAA 1 cut(s) 350
Tru9I TTAA 1 cut(s) 350
TscAI CASTG 3 cut(s) 148, 235, 474
TseI GCWGC 5 cut(s) 205, 356, 401, 404, 626
TspDTI ATGAA 1 cut(s) 328
TspGWI ACGGA 2 cut(s) 30, 263
TspRI CASTG 3 cut(s) 148, 235, 474
VneI GTGCAC 1 cut(s) 276
VpaK11BI GGWCC 1 cut(s) 64
XbaI TCTAGA 1 cut(s) 221
XceI RCATGY 1 cut(s) 650
XspI CTAG 3 cut(s) 188, 222, 642
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.