MD09G1269200.v1.1

Serine hydroxymethyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0018495
Physical Location & Seq
Forward (+)
34347070 .. 34356239
9170 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1269200.v1.1.491

Sequence Viewer

Length: 225 bp
ATGTTCTCAGAAGTCTATGGCAGGAATCGTTCTGGTAATGGATGCAGTTTTGCAGACAATGGGTTAAGTGAGGCTGACCCGGAGGTGCTGACGGAGGCAGTTAGCTCATGTCTCACGAACAAGTATTCAAAAGGATTACCAAGTAAAAGGTACTATGGTGGCAATGAGCATATTGATGAGCTTGAAACACTATGCCAAAGAGGGCTCTTGATGCATTTCACTTAG

Protein Analysis

75

Amino Acids

8.2

Weight (kDa)

5.19

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SHMT PF00464 30 - 70 3.5e-14 Serine hydroxymethyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018495)

Species Orthologous Gene IDs
malus_domestica MD09G1269200.v1.1
pyrus_communis pycom17g26660
rosa_chinensis RchiOBHm_Chr1g0360671 RchiOBHm_Chr7g0223221
rosa_multiflora Rmu_co8421897.1_g000001
rosa_roxburghii Rroxscaffold_4G00301080 Rroxscaffold_7G00212560
rosa_samantha Rh3BG358300 Rh6AG220400
rosa_wichuraiana Rw6G019320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 152
AgsI TTSAA 2 cut(s) 129, 185
AluBI AGCT 2 cut(s) 105, 181
AluI AGCT 2 cut(s) 105, 181
Alw26I GTCTC 1 cut(s) 116
AsuC2I CCSGG 1 cut(s) 80
BaeI ACNNNNGTAYC 2 cut(s) 142, 175
BanII GRGCYC 1 cut(s) 207
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 1 cut(s) 116
Bme1390I CCNGG 1 cut(s) 80
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 2 cut(s) 32, 201
BpuMI CCSGG 1 cut(s) 80
Bse3DI GCAATG 1 cut(s) 169
BseGI GGATG 1 cut(s) 47
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 1 cut(s) 21
BsiSI CCGG 1 cut(s) 80
BsmAI GTCTC 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 207
BspCNI CTCAG 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 169
BstDEI CTNAG 2 cut(s) 7, 222
BstF5I GGATG 1 cut(s) 47
BstMAI GTCTC 1 cut(s) 116
BstMWI GCNNNNNNNGC 1 cut(s) 211
BstSCI CCNGG 1 cut(s) 78
BtsCI GGATG 1 cut(s) 47
Csp6I GTAC 1 cut(s) 151
CviAII CATG 1 cut(s) 108
CviJI RGCY 4 cut(s) 74, 105, 181, 205
CviKI_1 RGCY 4 cut(s) 74, 105, 181, 205
CviQI GTAC 1 cut(s) 151
DdeI CTNAG 2 cut(s) 7, 222
Eco24I GRGCYC 1 cut(s) 207
EcoT22I ATGCAT 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 207
FaeI CATG 1 cut(s) 111
FaiI YATR 5 cut(s) 18, 109, 156, 171, 193
FatI CATG 1 cut(s) 107
FokI GGATG 1 cut(s) 54
FriOI GRGCYC 1 cut(s) 207
HapII CCGG 1 cut(s) 80
Hin1II CATG 1 cut(s) 111
HinfI GANTC 1 cut(s) 25
HpaII CCGG 1 cut(s) 80
Hpy188I TCNGA 1 cut(s) 10
Hpy188III TCNNGA 2 cut(s) 115, 208
HpyCH4V TGCA 3 cut(s) 45, 53, 214
HpyF10VI GCNNNNNNNGC 1 cut(s) 211
HpyF3I CTNAG 2 cut(s) 7, 222
Hsp92II CATG 1 cut(s) 111
LpnPI CCDG 3 cut(s) 7, 18, 93
LweI GCATC 2 cut(s) 32, 201
MhlI GDGCHC 1 cut(s) 207
MnlI CCTC 4 cut(s) 64, 76, 88, 194
Mph1103I ATGCAT 1 cut(s) 216
MseI TTAA 1 cut(s) 65
MslI CAYNNNNRTG 1 cut(s) 174
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 80
MwoI GCNNNNNNNGC 1 cut(s) 211
NciI CCSGG 1 cut(s) 80
NlaIII CATG 1 cut(s) 111
NsiI ATGCAT 1 cut(s) 216
PfeI GAWTC 1 cut(s) 25
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
RseI CAYNNNNRTG 1 cut(s) 174
SaqAI TTAA 1 cut(s) 65
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 1 cut(s) 207
SetI ASST 4 cut(s) 87, 107, 152, 183
SfaNI GCATC 2 cut(s) 32, 201
SmiMI CAYNNNNRTG 1 cut(s) 174
StyD4I CCNGG 1 cut(s) 78
TfiI GAWTC 1 cut(s) 25
Tru1I TTAA 1 cut(s) 65
Tru9I TTAA 1 cut(s) 65
TspGWI ACGGA 1 cut(s) 107
Zsp2I ATGCAT 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.