Rh3BG358300

Serine hydroxymethyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0018495
Physical Location & Seq
Reverse (-)
42874634 .. 42880053
5420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG358300.1

Sequence Viewer

Length: 240 bp
ATGGAGGCAGTTGGCTCATGTCTCAGCAACAAGTATTCGGAAGGATTACCTGGGAAAAGGTACTATGGTGGAAATGAGCACATTGATGAGCTTGAAACACTAAGCCAAAAAAGGGCCCTGGATACATTTCACCTAGATGGAAAGAAGTGGGGCGTCAATGTTCAACCATTATCTGGTTCTCTAGCTAATTTCGAGGTTTATATAGCAGTTCTTAATCCTCATGATCATATCATGGAGTAG

Protein Analysis

79

Amino Acids

8.82

Weight (kDa)

5.45

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SHMT PF00464 1 - 78 7.9e-37 Serine hydroxymethyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018495)

Species Orthologous Gene IDs
malus_domestica MD09G1269200.v1.1
pyrus_communis pycom17g26660
rosa_chinensis RchiOBHm_Chr1g0360671 RchiOBHm_Chr7g0223221
rosa_multiflora Rmu_co8421897.1_g000001
rosa_roxburghii Rroxscaffold_4G00301080 Rroxscaffold_7G00212560
rosa_samantha Rh3BG358300 Rh6AG220400
rosa_wichuraiana Rw6G019320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 173
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 1 cut(s) 62
AfiI CCNNNNNNNGG 2 cut(s) 112, 173
AgsI TTSAA 2 cut(s) 95, 164
AjnI CCWGG 2 cut(s) 49, 117
AluBI AGCT 2 cut(s) 91, 185
AluI AGCT 2 cut(s) 91, 185
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 1 cut(s) 26
AoxI GGCC 1 cut(s) 114
ApaI GGGCCC 1 cut(s) 118
AspS9I GGNCC 2 cut(s) 114, 115
AsuHPI GGTGA 1 cut(s) 122
BaeGI GKGCMC 1 cut(s) 118
BaeI ACNNNNGTAYC 2 cut(s) 52, 85
BanII GRGCYC 1 cut(s) 118
Bbv12I GWGCWC 1 cut(s) 81
BccI CCATC 1 cut(s) 131
BciT130I CCWGG 2 cut(s) 51, 119
BciVI GTATCC 1 cut(s) 115
BclI TGATCA 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 26
BfaI CTAG 2 cut(s) 134, 182
BfuI GTATCC 1 cut(s) 115
Bme1390I CCNGG 2 cut(s) 51, 119
BmgT120I GGNCC 2 cut(s) 114, 115
BmiI GGNNCC 1 cut(s) 116
BmrFI CCNGG 2 cut(s) 51, 119
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 50, 117
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 173
BseBI CCWGG 2 cut(s) 51, 119
BseDI CCNNGG 2 cut(s) 50, 117
BseLI CCNNNNNNNGG 2 cut(s) 112, 173
BseMII CTCAG 1 cut(s) 37
BseSI GKGCMC 1 cut(s) 118
BshFI GGCC 1 cut(s) 116
BsiHKAI GWGCWC 1 cut(s) 81
BslI CCNNNNNNNGG 2 cut(s) 112, 173
BsmAI GTCTC 1 cut(s) 26
BsnI GGCC 1 cut(s) 116
Bsp120I GGGCCC 1 cut(s) 114
Bsp1286I GDGCHC 2 cut(s) 81, 118
Bsp143I GATC 1 cut(s) 223
BspANI GGCC 1 cut(s) 116
BspCNI CTCAG 1 cut(s) 36
BspHI TCATGA 1 cut(s) 220
BspLI GGNNCC 1 cut(s) 116
BssECI CCNNGG 2 cut(s) 50, 117
BssMI GATC 1 cut(s) 223
BssNI GRCGYC 1 cut(s) 153
Bst2UI CCWGG 2 cut(s) 51, 119
BstACI GRCGYC 1 cut(s) 153
BstDEI CTNAG 2 cut(s) 23, 101
BstKTI GATC 1 cut(s) 226
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 1 cut(s) 223
BstNI CCWGG 2 cut(s) 51, 119
BstSCI CCNGG 2 cut(s) 49, 117
BstSLI GKGCMC 1 cut(s) 118
BsuI GTATCC 1 cut(s) 115
BsuRI GGCC 1 cut(s) 116
CciI TCATGA 1 cut(s) 220
Cfr13I GGNCC 2 cut(s) 114, 115
CseI GACGC 1 cut(s) 142
Csp6I GTAC 1 cut(s) 61
CviAII CATG 3 cut(s) 18, 221, 232
CviJI RGCY 5 cut(s) 15, 91, 105, 116, 185
CviKI_1 RGCY 5 cut(s) 15, 91, 105, 116, 185
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 2 cut(s) 23, 101
DpnI GATC 1 cut(s) 225
DpnII GATC 1 cut(s) 223
Eco24I GRGCYC 1 cut(s) 118
EcoO109I RGGNCCY 2 cut(s) 114, 115
EcoRII CCWGG 2 cut(s) 49, 117
EcoT38I GRGCYC 1 cut(s) 118
FaeI CATG 3 cut(s) 21, 224, 235
FaiI YATR 7 cut(s) 19, 66, 201, 203, 222, 228, 233
FatI CATG 3 cut(s) 17, 220, 231
FbaI TGATCA 1 cut(s) 223
FriOI GRGCYC 1 cut(s) 118
FspBI CTAG 2 cut(s) 134, 182
HaeIII GGCC 1 cut(s) 116
HgaI GACGC 1 cut(s) 142
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 3 cut(s) 21, 224, 235
HphI GGTGA 1 cut(s) 122
Hpy188I TCNGA 1 cut(s) 40
Hpy188III TCNNGA 1 cut(s) 221
HpyAV CCTTC 1 cut(s) 35
HpyF3I CTNAG 2 cut(s) 23, 101
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 3 cut(s) 21, 224, 235
Ksp22I TGATCA 1 cut(s) 223
Kzo9I GATC 1 cut(s) 223
LpnPI CCDG 5 cut(s) 36, 63, 104, 131, 159
MaeI CTAG 2 cut(s) 134, 182
MalI GATC 1 cut(s) 225
MboI GATC 1 cut(s) 223
MhlI GDGCHC 2 cut(s) 81, 118
MluCI AATT 1 cut(s) 187
MnlI CCTC 2 cut(s) 187, 228
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 2 cut(s) 84, 135
MspR9I CCNGG 2 cut(s) 51, 119
MvaI CCWGG 2 cut(s) 51, 119
NdeII GATC 1 cut(s) 223
NlaIII CATG 3 cut(s) 21, 224, 235
NlaIV GGNNCC 1 cut(s) 116
PagI TCATGA 1 cut(s) 220
PflMI CCANNNNNTGG 1 cut(s) 173
Psp6I CCWGG 2 cut(s) 49, 117
PspGI CCWGG 2 cut(s) 49, 117
PspN4I GGNNCC 1 cut(s) 116
PspOMI GGGCCC 1 cut(s) 114
PspPI GGNCC 2 cut(s) 114, 115
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
RseI CAYNNNNRTG 2 cut(s) 84, 135
SaqAI TTAA 1 cut(s) 213
Sau3AI GATC 1 cut(s) 223
Sau96I GGNCC 2 cut(s) 114, 115
ScrFI CCNGG 2 cut(s) 51, 119
SduI GDGCHC 2 cut(s) 81, 118
SetI ASST 6 cut(s) 52, 62, 93, 135, 187, 198
SmiMI CAYNNNNRTG 2 cut(s) 84, 135
Sse9I AATT 1 cut(s) 187
SspMI CTAG 2 cut(s) 134, 182
StyD4I CCNGG 2 cut(s) 49, 117
TaqI TCGA 1 cut(s) 192
TasI AATT 1 cut(s) 187
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
Van91I CCANNNNNTGG 1 cut(s) 173
XspI CTAG 2 cut(s) 134, 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.