Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004108

Physical Location & Seq

Genomic Coordinates Reverse (-)

34407775 .. 34411198

Gene Structure 3424 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1269800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 429 bp

ATGGAATCTGAGATGGTAGTGCATGAAGGCGGGTGCCACTGCAAGAATGTAAGGTGGCAAGTGAAGGCGCCTTGCGGTGTTGTGGCTTGGAGTTGCAACTGCTCAAATTGCTTTATGAGGGGTAACACCCATTTCATTGTCCCTGCTGAAAGATTTCAACTTTTGAGTGATTCTGAGCAGTTTCTTACAACTTACAGCTTCGGTACTCATACGGCAAAGCACACATTCTGTAAAGTTTGTGGGATAACTTCGTTCTATTATCCACGCTCAAACCCTGATGGGGTCGCCATCACATTTAGGTGTGTTGATCCTGGAACACTCGCCCATGTTGAGATTAAGTATTACGATGGGCAAAATTGGGAAGCCTCGCACAGTCAATCAGGCATTGCGTCGTTGTCAAAAGTGCAGACTCTACAAAATATAGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000775 GO:0000776 GO:0001667 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005819 GO:0005856 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006928 GO:0006996 GO:0008150 GO:0009987 GO:0010564 GO:0015630 GO:0016043 GO:0016477 GO:0022607 GO:0031055 GO:0031497 GO:0031507 GO:0031508 GO:0032465 GO:0032467 GO:0032991 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0040011 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044446 GO:0044464 GO:0045787 GO:0048518 GO:0048522 GO:0048870 GO:0050789 GO:0050794 GO:0051128 GO:0051179 GO:0051233 GO:0051276 GO:0051302 GO:0051674 GO:0051726 GO:0051781 GO:0065003 GO:0065004 GO:0065007 GO:0070828 GO:0071103 GO:0071824 GO:0071840 GO:0090068 GO:0098687

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GFA

Protein Analysis

143

Amino Acids

15.83

Weight (kDa)

7.02

Isoelectric Point (pI)

37.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GFA	PF04828	8 - 105	3.1e-10	Glutathione-dependent formaldehyde-activating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0004108)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16940 AT5G16940
fragaria_vesca	FvH4_1g30100 FvH4_1g30100 FvH4_1g30100
malus_domestica	MD09G1269100.v1.1 MD09G1269600.v1.1 MD09G1269800.v1.1 MD17G1267500.v1.1 MD17G1267700.v1.1
prunus_persica	Prupe.3G165600_v2.0.a1 Prupe.3G165600_v2.0.a1
pyrus_communis	pycom09g18100
rosa_chinensis	RchiOBHm_Chr3g0491661 RchiOBHm_Chr3g0491691 RchiOBHm_Chr3g0491731 RchiOBHm_Chr3g0491901
rosa_laevigata	RLG00000022814 RLG00000022830
rosa_multiflora	Rmu_sc0003786.1_g000006 Rmu_sc0009763.1_g000004 Rmu_sc0033062.1_g000001
rosa_roxburghii	Rroxscaffold_6G00392720
rosa_rugosa	Rorug03G0250800
rosa_samantha	Rh3AG299600 Rh3AG300400 Rh3BG336400 Rh3CG332600 Rh3CG333600 Rh3DG334400 Rh3DG335600
rosa_wichuraiana	Rw3G026480 Rw3G026550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	33, 67
AciI	CCGC	2 cut(s)	30, 75
AclWI	GGATC	1 cut(s)	302
AcyI	GRCGYC	1 cut(s)	68
AfaI	GTAC	1 cut(s)	205
AfiI	CCNNNNNNNGG	1 cut(s)	280
AgsI	TTSAA	1 cut(s)	158
AjnI	CCWGG	1 cut(s)	310
AluBI	AGCT	1 cut(s)	198
AluI	AGCT	1 cut(s)	198
AlwI	GGATC	1 cut(s)	302
Asp700I	GAANNNNTTC	1 cut(s)	153
AspLEI	GCGC	1 cut(s)	70
BanI	GGYRCC	2 cut(s)	33, 67
BccI	CCATC	4 cut(s)	7, 272, 296, 341
BceAI	ACGGC	1 cut(s)	228
BciT130I	CCWGG	1 cut(s)	312
BfoI	RGCGCY	1 cut(s)	71
Bme1390I	CCNGG	1 cut(s)	312
BmiI	GGNNCC	2 cut(s)	35, 69
BmrFI	CCNGG	1 cut(s)	312
BsaHI	GRCGYC	1 cut(s)	68
Bsc4I	CCNNNNNNNGG	1 cut(s)	280
Bse3DI	GCAATG	1 cut(s)	384
BseBI	CCWGG	1 cut(s)	312
BseLI	CCNNNNNNNGG	1 cut(s)	280
BseMI	GCAATG	1 cut(s)	384
BseMII	CTCAG	1 cut(s)	165
BsgI	GTGCAG	1 cut(s)	425
BshNI	GGYRCC	2 cut(s)	33, 67
BslFI	GGGAC	1 cut(s)	125
BslI	CCNNNNNNNGG	1 cut(s)	280
BsmFI	GGGAC	1 cut(s)	125
Bsp143I	GATC	1 cut(s)	307
BspACI	CCGC	2 cut(s)	30, 75
BspCNI	CTCAG	1 cut(s)	166
BspLI	GGNNCC	2 cut(s)	35, 69
BspPI	GGATC	1 cut(s)	302
BspT107I	GGYRCC	2 cut(s)	33, 67
BsrDI	GCAATG	1 cut(s)	384
BssMI	GATC	1 cut(s)	307
BssNI	GRCGYC	1 cut(s)	68
Bst2UI	CCWGG	1 cut(s)	312
Bst4CI	ACNGT	1 cut(s)	374
BstACI	GRCGYC	1 cut(s)	68
BstDEI	CTNAG	2 cut(s)	9, 174
BstH2I	RGCGCY	1 cut(s)	71
BstHHI	GCGC	1 cut(s)	70
BstKTI	GATC	1 cut(s)	310
BstMBI	GATC	1 cut(s)	307
BstMWI	GCNNNNNNNGC	1 cut(s)	108
BstNI	CCWGG	1 cut(s)	312
BstSCI	CCNGG	1 cut(s)	310
BtsI	GCAGTG	1 cut(s)	37
BtsIMutI	CAGTG	1 cut(s)	37
CfoI	GCGC	1 cut(s)	70
CseI	GACGC	1 cut(s)	378
Csp6I	GTAC	1 cut(s)	204
CviAII	CATG	2 cut(s)	23, 326
CviJI	RGCY	3 cut(s)	86, 198, 365
CviKI_1	RGCY	3 cut(s)	86, 198, 365
CviQI	GTAC	1 cut(s)	204
DdeI	CTNAG	2 cut(s)	9, 174
DinI	GGCGCC	1 cut(s)	69
DpnI	GATC	1 cut(s)	309
DpnII	GATC	1 cut(s)	307
EcoRII	CCWGG	1 cut(s)	310
EgeI	GGCGCC	1 cut(s)	69
EheI	GGCGCC	1 cut(s)	69
FaeI	CATG	2 cut(s)	26, 329
FaiI	YATR	5 cut(s)	24, 116, 210, 327, 422
FaqI	GGGAC	1 cut(s)	125
FatI	CATG	2 cut(s)	22, 325
FauI	CCCGC	1 cut(s)	23
GlaI	GCGC	1 cut(s)	69
HaeII	RGCGCY	1 cut(s)	71
HgaI	GACGC	1 cut(s)	378
HhaI	GCGC	1 cut(s)	70
Hin1I	GRCGYC	1 cut(s)	68
Hin1II	CATG	2 cut(s)	26, 329
Hin6I	GCGC	1 cut(s)	68
HinP1I	GCGC	1 cut(s)	68
HinfI	GANTC	3 cut(s)	5, 170, 409
Hpy188I	TCNGA	2 cut(s)	10, 175
Hpy99I	CGWCG	1 cut(s)	394
HpyAV	CCTTC	2 cut(s)	20, 58
HpyCH4III	ACNGT	1 cut(s)	374
HpyCH4V	TGCA	4 cut(s)	22, 42, 96, 406
HpyF10VI	GCNNNNNNNGC	1 cut(s)	108
HpyF3I	CTNAG	2 cut(s)	9, 174
Hsp92I	GRCGYC	1 cut(s)	68
Hsp92II	CATG	2 cut(s)	26, 329
HspAI	GCGC	1 cut(s)	68
KasI	GGCGCC	1 cut(s)	67
Kzo9I	GATC	1 cut(s)	307
LpnPI	CCDG	5 cut(s)	156, 288, 297, 324, 366
MaeIII	GTNAC	1 cut(s)	122
MalI	GATC	1 cut(s)	309
MboI	GATC	1 cut(s)	307
MluCI	AATT	2 cut(s)	106, 355
Mly113I	GGCGCC	1 cut(s)	68
MlyI	GAGTC	1 cut(s)	403
MnlI	CCTC	2 cut(s)	111, 376
MroXI	GAANNNNTTC	1 cut(s)	153
MseI	TTAA	1 cut(s)	336
MslI	CAYNNNNRTG	1 cut(s)	298
MspR9I	CCNGG	1 cut(s)	312
MvaI	CCWGG	1 cut(s)	312
MwoI	GCNNNNNNNGC	1 cut(s)	108
NarI	GGCGCC	1 cut(s)	68
NdeII	GATC	1 cut(s)	307
NlaIII	CATG	2 cut(s)	26, 329
NlaIV	GGNNCC	2 cut(s)	35, 69
PdmI	GAANNNNTTC	1 cut(s)	153
PfeI	GAWTC	2 cut(s)	5, 170
PfoI	TCCNGGA	1 cut(s)	310
PleI	GAGTC	1 cut(s)	403
PluTI	GGCGCC	1 cut(s)	71
PpsI	GAGTC	1 cut(s)	403
Psp6I	CCWGG	1 cut(s)	310
PspGI	CCWGG	1 cut(s)	310
PspN4I	GGNNCC	2 cut(s)	35, 69
RsaI	GTAC	1 cut(s)	205
RsaNI	GTAC	1 cut(s)	204
RseI	CAYNNNNRTG	1 cut(s)	298
SaqAI	TTAA	1 cut(s)	336
Sau3AI	GATC	1 cut(s)	307
SchI	GAGTC	1 cut(s)	403
ScrFI	CCNGG	1 cut(s)	312
SetI	ASST	4 cut(s)	56, 200, 302, 427
SfoI	GGCGCC	1 cut(s)	69
SmiMI	CAYNNNNRTG	1 cut(s)	298
Sse9I	AATT	2 cut(s)	106, 355
SsiI	CCGC	2 cut(s)	30, 75
SspDI	GGCGCC	1 cut(s)	67
StyD4I	CCNGG	1 cut(s)	310
TaaI	ACNGT	1 cut(s)	374
TasI	AATT	2 cut(s)	106, 355
TfiI	GAWTC	2 cut(s)	5, 170
Tru1I	TTAA	1 cut(s)	336
Tru9I	TTAA	1 cut(s)	336
TscAI	CASTG	1 cut(s)	44
TspDTI	ATGAA	2 cut(s)	39, 124
TspRI	CASTG	1 cut(s)	44
XmnI	GAANNNNTTC	1 cut(s)	153

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.