Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004108

Physical Location & Seq

Genomic Coordinates Reverse (-)

32758237 .. 32758632

Gene Structure 396 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1267500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 396 bp

ATGATAATACGGGACGGGCTGTCACACATGGACTCTACAATGGTAGTACATGAAGGCGGGTGCCACTGCAAGAATGTAAGATGGCGAGTGAAGGCACCTAGCAGTGTTGTGGCTACGAGTTGCAACTGCTCGAATTGCTCTATAAGGGGTACCATCGCTTTCGTTGTCCCTGCTGAAAGATTTAAACTTTGGGGTGATTCTGAGCAGTTTCTTACAACCAACACCTTCGGTACTCATACTACAAAGCACATGTTCTGTAAAGTTTGTGGGATAACTTCATTCTATTATCAACGCGAGAACCCTGATGGGGTCACAATCACGTTTAGGTGTGTTGATCCTGGAACACTCACTTGCGTTGAGATTAAGTATTTCGACGGGAAAAATTGGGAAGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000775 GO:0000776 GO:0001667 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005819 GO:0005856 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006928 GO:0006996 GO:0008150 GO:0009987 GO:0010564 GO:0015630 GO:0016043 GO:0016477 GO:0022607 GO:0031055 GO:0031497 GO:0031507 GO:0031508 GO:0032465 GO:0032467 GO:0032991 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0040011 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044430 GO:0044446 GO:0044464 GO:0045787 GO:0048518 GO:0048522 GO:0048870 GO:0050789 GO:0050794 GO:0051128 GO:0051179 GO:0051233 GO:0051276 GO:0051302 GO:0051674 GO:0051726 GO:0051781 GO:0065003 GO:0065004 GO:0065007 GO:0070828 GO:0071103 GO:0071824 GO:0071840 GO:0090068 GO:0098687

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GFA

Protein Analysis

132

Amino Acids

14.75

Weight (kDa)

8.09

Isoelectric Point (pI)

27.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GFA	PF04828	17 - 113	6.6e-12	Glutathione-dependent formaldehyde-activating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0004108)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16940 AT5G16940
fragaria_vesca	FvH4_1g30100 FvH4_1g30100 FvH4_1g30100
malus_domestica	MD09G1269100.v1.1 MD09G1269600.v1.1 MD09G1269800.v1.1 MD17G1267500.v1.1 MD17G1267700.v1.1
prunus_persica	Prupe.3G165600_v2.0.a1 Prupe.3G165600_v2.0.a1
pyrus_communis	pycom09g18100
rosa_chinensis	RchiOBHm_Chr3g0491661 RchiOBHm_Chr3g0491691 RchiOBHm_Chr3g0491731 RchiOBHm_Chr3g0491901
rosa_laevigata	RLG00000022814 RLG00000022830
rosa_multiflora	Rmu_sc0003786.1_g000006 Rmu_sc0009763.1_g000004 Rmu_sc0033062.1_g000001
rosa_roxburghii	Rroxscaffold_6G00392720
rosa_rugosa	Rorug03G0250800
rosa_samantha	Rh3AG299600 Rh3AG300400 Rh3BG336400 Rh3CG332600 Rh3CG333600 Rh3DG334400 Rh3DG335600
rosa_wichuraiana	Rw3G026480 Rw3G026550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	149
AccB1I	GGYRCC	3 cut(s)	60, 94, 149
AccII	CGCG	1 cut(s)	294
AciI	CCGC	1 cut(s)	57
AclWI	GGATC	1 cut(s)	329
AfaI	GTAC	3 cut(s)	48, 151, 232
AfiI	CCNNNNNNNGG	1 cut(s)	307
AflIII	ACRYGT	1 cut(s)	249
AhdI	GACNNNNNGTC	1 cut(s)	19
AjnI	CCWGG	1 cut(s)	337
AlwI	GGATC	1 cut(s)	329
Asp718I	GGTACC	1 cut(s)	149
AsuHPI	GGTGA	1 cut(s)	206
BanI	GGYRCC	3 cut(s)	60, 94, 149
BccI	CCATC	3 cut(s)	75, 161, 299
BciT130I	CCWGG	1 cut(s)	339
BfaI	CTAG	1 cut(s)	99
Bme1390I	CCNGG	1 cut(s)	339
BmeRI	GACNNNNNGTC	1 cut(s)	19
BmiI	GGNNCC	3 cut(s)	62, 96, 151
BmrFI	CCNGG	1 cut(s)	339
Bsc4I	CCNNNNNNNGG	1 cut(s)	307
BseBI	CCWGG	1 cut(s)	339
BseLI	CCNNNNNNNGG	1 cut(s)	307
BseMII	CTCAG	1 cut(s)	192
Bsh1236I	CGCG	1 cut(s)	294
BshNI	GGYRCC	3 cut(s)	60, 94, 149
BslFI	GGGAC	2 cut(s)	26, 152
BslI	CCNNNNNNNGG	1 cut(s)	307
BsmFI	GGGAC	2 cut(s)	26, 152
Bsp143I	GATC	1 cut(s)	334
BspACI	CCGC	1 cut(s)	57
BspCNI	CTCAG	1 cut(s)	193
BspFNI	CGCG	1 cut(s)	294
BspLI	GGNNCC	3 cut(s)	62, 96, 151
BspPI	GGATC	1 cut(s)	329
BspT107I	GGYRCC	3 cut(s)	60, 94, 149
BssMI	GATC	1 cut(s)	334
Bst2UI	CCWGG	1 cut(s)	339
BstDEI	CTNAG	1 cut(s)	201
BstFNI	CGCG	1 cut(s)	294
BstKTI	GATC	1 cut(s)	337
BstMBI	GATC	1 cut(s)	334
BstMWI	GCNNNNNNNGC	1 cut(s)	135
BstNI	CCWGG	1 cut(s)	339
BstNSI	RCATGY	1 cut(s)	253
BstSCI	CCNGG	1 cut(s)	337
BstUI	CGCG	1 cut(s)	294
BtgZI	GCGATG	1 cut(s)	139
BtsI	GCAGTG	2 cut(s)	64, 109
BtsIMutI	CAGTG	2 cut(s)	64, 109
Csp6I	GTAC	3 cut(s)	47, 150, 231
CviAII	CATG	3 cut(s)	28, 50, 250
CviJI	RGCY	2 cut(s)	19, 113
CviKI_1	RGCY	2 cut(s)	19, 113
CviQI	GTAC	3 cut(s)	47, 150, 231
DdeI	CTNAG	1 cut(s)	201
DpnI	GATC	1 cut(s)	336
DpnII	GATC	1 cut(s)	334
DraI	TTTAAA	1 cut(s)	184
DriI	GACNNNNNGTC	1 cut(s)	19
Eam1105I	GACNNNNNGTC	1 cut(s)	19
EcoRII	CCWGG	1 cut(s)	337
FaeI	CATG	3 cut(s)	31, 53, 253
FaiI	YATR	5 cut(s)	29, 51, 143, 237, 251
FaqI	GGGAC	2 cut(s)	26, 152
FatI	CATG	3 cut(s)	27, 49, 249
FauI	CCCGC	1 cut(s)	50
FspBI	CTAG	1 cut(s)	99
Hin1II	CATG	3 cut(s)	31, 53, 253
HinfI	GANTC	2 cut(s)	32, 197
HphI	GGTGA	1 cut(s)	206
Hpy188I	TCNGA	1 cut(s)	202
Hpy99I	CGWCG	1 cut(s)	377
HpyAV	CCTTC	3 cut(s)	47, 85, 235
HpyCH4IV	ACGT	1 cut(s)	320
HpyCH4V	TGCA	2 cut(s)	69, 123
HpyF10VI	GCNNNNNNNGC	1 cut(s)	135
HpyF3I	CTNAG	1 cut(s)	201
HpySE526I	ACGT	1 cut(s)	320
Hsp92II	CATG	3 cut(s)	31, 53, 253
KpnI	GGTACC	1 cut(s)	153
Kzo9I	GATC	1 cut(s)	334
LpnPI	CCDG	4 cut(s)	183, 315, 324, 351
MaeI	CTAG	1 cut(s)	99
MaeII	ACGT	1 cut(s)	320
MaeIII	GTNAC	2 cut(s)	21, 310
MalI	GATC	1 cut(s)	336
MboI	GATC	1 cut(s)	334
MluCI	AATT	2 cut(s)	133, 382
MlyI	GAGTC	1 cut(s)	26
MseI	TTAA	2 cut(s)	183, 363
MspR9I	CCNGG	1 cut(s)	339
MvaI	CCWGG	1 cut(s)	339
MvnI	CGCG	1 cut(s)	294
MwoI	GCNNNNNNNGC	1 cut(s)	135
NdeII	GATC	1 cut(s)	334
NlaIII	CATG	3 cut(s)	31, 53, 253
NlaIV	GGNNCC	3 cut(s)	62, 96, 151
NmuCI	GTSAC	2 cut(s)	21, 310
NspI	RCATGY	1 cut(s)	253
PciI	ACATGT	1 cut(s)	249
PfeI	GAWTC	1 cut(s)	197
PfoI	TCCNGGA	1 cut(s)	337
PleI	GAGTC	1 cut(s)	26
PpsI	GAGTC	1 cut(s)	26
PscI	ACATGT	1 cut(s)	249
Psp6I	CCWGG	1 cut(s)	337
PspGI	CCWGG	1 cut(s)	337
PspN4I	GGNNCC	3 cut(s)	62, 96, 151
RsaI	GTAC	3 cut(s)	48, 151, 232
RsaNI	GTAC	3 cut(s)	47, 150, 231
SaqAI	TTAA	2 cut(s)	183, 363
Sau3AI	GATC	1 cut(s)	334
SchI	GAGTC	1 cut(s)	26
ScrFI	CCNGG	1 cut(s)	339
SetI	ASST	4 cut(s)	100, 227, 323, 329
Sse9I	AATT	2 cut(s)	133, 382
SsiI	CCGC	1 cut(s)	57
SspMI	CTAG	1 cut(s)	99
StyD4I	CCNGG	1 cut(s)	337
TaiI	ACGT	1 cut(s)	323
TaqI	TCGA	2 cut(s)	131, 372
TasI	AATT	2 cut(s)	133, 382
TatI	WGTACW	1 cut(s)	46
TfiI	GAWTC	1 cut(s)	197
Tru1I	TTAA	2 cut(s)	183, 363
Tru9I	TTAA	2 cut(s)	183, 363
TscAI	CASTG	2 cut(s)	71, 109
TseFI	GTSAC	2 cut(s)	21, 310
Tsp45I	GTSAC	2 cut(s)	21, 310
TspDTI	ATGAA	2 cut(s)	66, 267
TspRI	CASTG	2 cut(s)	71, 109
XceI	RCATGY	1 cut(s)	253
XspI	CTAG	1 cut(s)	99

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.