MD09G1270000.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015933
Physical Location & Seq
Forward (+)
34432318 .. 34433745
1428 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1270000.v1.1.491

Sequence Viewer

Length: 1428 bp
ATGTACCTCTCTCTCAAAACCATGTTCTTTAATCTTATCACGATCTTCATCTTCTTCCTCGTCTCTCCTTCTCATTCGATTTCCATCCCGAAAGATGATCAGAACAAGCACCAAAACAAGTTGAACAACAACGACACTAGTGCTTTTTCTCTCTCTTTCCCTCTCACTACTTCTCCACTTTCTCTCTCTCCTCCAAACACGTCCAAAACATTCCATGCCTCTCTCGTTTCAAACTCCAAGCAAAACCCTAGACTTTCCAGAAGATCGGCTGGTGGTGCTACAGTAAAAAATCCGTCGTACAACTACAAGATTTCGTTCAAGTATTCAATGGCTCTGATCGTTTCTCTCCCTATCGGAACTCCGCCGCAGACCCAACAGATGGTTTTGGACACCGGAAGCCAACTCTCATGGATTCAGTGCCACAACAAGACCCCCAAACCACGGCCACCTCCTCCTCCCACAGCGTCTTTTGACCCTTCTCTCTCTTCCACTTTCTCTGTTTTGCCTTGCACTCACCCTATTTGCAAACCCCGGGTTCCCGATTTTACCCTCCCAACCTCTTGCGACCGAAACCGCCTCTGCCACTATTCCTACTTCTATGCCGACGGTACTCTCGCTGAGGGCAATCTCATCCGAGAAAAATTCACCTTTTCTCGTTCCGTAAGTACCCCTCCCTTGATTCTTGGTTGTGCTAAGGACACCAGTGACACCAAGGGTATTCTGGGTATGAATCTTGGGCGGCTATCATTCGCTTCCCAAGCAAAAGTTACCAAGTTCTCCTACTGCGTACCTAACCGACCGGCTCGAACCGGGTCCAATATGCCGACCGGGAGTTTCTACTTGGGCCACAACCCGAATTCTGCGGGATTCCGATACGTTGACCTTTTGACCTTTGGTCAAAGTCAACGCATGCCGAATTTGGACCCCCTTGCCTATACAGTTGTAATGGTGGGGATAAGAATTGGTGGGAAAATGTTGAAAATTCAACCCTCCATATTCCGACCCGATGCCAGCGGGGCGGGTCAGACTATGATTGATTCAGGTTCTGAGTTCACTTATTTTGTGGATGAAGCGTATAGTAAGGTGAGGGAAGAGATTGTGAGACTGGCGGGAGCAAAGTTGAAAAAGGGTTACGTGTATGCAGGTGTGTCCGACATGTGTTTTGATGGTGATAGCATGGAGATCGGGCGGTTGATAGGAGACATGGCATTTGTGTTTGCTAAAGGTGTGGAGATTGTGACGCCAAAAGAACAAATTCTAGCCGAAGTAGGGCGCGGAGTCCGTTGCGTTGCAGTCGGACAGTCCAACATGCTTGGTGCCTCCAGCAATATAATTGGAAATTTTCATCAGAGGAATCAATGGGTTGAATTTGATCTGGCAAATCGTAGAGTTGGGTTCGGTAGGGCTGATTGTAGTAGATCAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

476

Amino Acids

52.17

Weight (kDa)

9.53

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 113 - 271 9.8e-34 Xylanase inhibitor N-terminal
Asp PF00026 116 - 468 3.9e-13 Eukaryotic aspartyl protease
TAXi_C PF14541 311 - 467 2.3e-32 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015933)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66180 AT5G37540
fragaria_vesca FvH4_1g30150
malus_domestica MD09G1270000.v1.1 MD17G1268100.v1.1
prunus_persica Prupe.3G165900_v2.0.a1
pyrus_communis pycom09g18120
rosa_chinensis RchiOBHm_Chr3g0491651
rosa_laevigata RLG00000022831
rosa_rugosa Rorug03G0250700.1
rosa_samantha Rh3AG299500 Rh3BG336200 Rh3CG332500 Rh3DG334100
rosa_wichuraiana Rw3G026460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1133
Acc36I ACCTGC 1 cut(s) 1133
AccB1I GGYRCC 1 cut(s) 1316
AccB7I CCANNNNNTGG 1 cut(s) 379
AccII CGCG 1 cut(s) 1275
AcoI YGGCCR 1 cut(s) 443
AcsI RAATTY 7 cut(s) 641, 856, 916, 981, 1254, 1339, 1367
AcyI GRCGYC 1 cut(s) 1241
AfaI GTAC 5 cut(s) 5, 299, 610, 667, 789
AfiI CCNNNNNNNGG 3 cut(s) 379, 441, 1269
AflIII ACRYGT 3 cut(s) 198, 1134, 1155
AgsI TTSAA 8 cut(s) 124, 231, 319, 327, 979, 986, 1123, 1367
AhdI GACNNNNNGTC 1 cut(s) 894
AhlI ACTAGT 1 cut(s) 137
AjiI CACGTC 1 cut(s) 201
Alw26I GTCTC 3 cut(s) 67, 1096, 1194
AlwNI CAGNNNCTG 1 cut(s) 1046
Ama87I CYCGRG 1 cut(s) 531
AoxI GGCC 2 cut(s) 443, 844
ApoI RAATTY 7 cut(s) 641, 856, 916, 981, 1254, 1339, 1367
ArsI GACNNNNNNTTYG 4 cut(s) 1145, 1177, 1193, 1225
Asp700I GAANNNNTTC 1 cut(s) 1254
AspLEI GCGC 1 cut(s) 1275
AspS9I GGNCC 3 cut(s) 813, 844, 922
AsuC2I CCSGG 4 cut(s) 532, 533, 811, 829
AsuHPI GGTGA 4 cut(s) 506, 637, 1096, 1181
AvaI CYCGRG 1 cut(s) 531
AvaII GGWCC 2 cut(s) 813, 922
BanI GGYRCC 1 cut(s) 1316
BbvCI CCTCAGC 1 cut(s) 618
BccI CCATC 3 cut(s) 92, 373, 1160
BceAI ACGGC 1 cut(s) 458
BcgI CGANNNNNNTGC 4 cut(s) 122, 156, 1165, 1199
BclI TGATCA 1 cut(s) 97
BcnI CCSGG 4 cut(s) 532, 533, 811, 829
BcoDI GTCTC 3 cut(s) 67, 1096, 1194
BcuI ACTAGT 1 cut(s) 137
BfaI CTAG 3 cut(s) 138, 249, 1259
BfmI CTRYAG 1 cut(s) 279
BfuAI ACCTGC 1 cut(s) 1133
BglI GCCNNNNNGGC 1 cut(s) 1016
BisI GCNGC 2 cut(s) 365, 740
BlsI GCNGC 2 cut(s) 366, 741
Bme1390I CCNGG 4 cut(s) 532, 533, 811, 829
Bme18I GGWCC 2 cut(s) 813, 922
BmeRI GACNNNNNGTC 1 cut(s) 894
BmeT110I CYCGRG 1 cut(s) 531
BmgBI CACGTC 1 cut(s) 201
BmgT120I GGNCC 3 cut(s) 813, 844, 922
BmiI GGNNCC 4 cut(s) 537, 814, 924, 1318
BmrFI CCNGG 4 cut(s) 532, 533, 811, 829
BmsI GCATC 1 cut(s) 997
BpmI CTGGAG 1 cut(s) 1306
Bpu10I CCTNAGC 2 cut(s) 618, 693
BpuMI CCSGG 4 cut(s) 532, 533, 811, 829
BsaAI YACGTR 1 cut(s) 1135
BsaBI GATNNNNATC 2 cut(s) 47, 83
BsaHI GRCGYC 1 cut(s) 1241
BsaJI CCNNGG 4 cut(s) 440, 530, 531, 711
BsaWI WCCGGW 1 cut(s) 392
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 3 cut(s) 379, 441, 1269
Bse118I RCCGGY 1 cut(s) 799
Bse1I ACTGG 2 cut(s) 702, 1110
Bse8I GATNNNNATC 2 cut(s) 47, 83
BseDI CCNNGG 4 cut(s) 440, 530, 531, 711
BseGI GGATG 3 cut(s) 84, 630, 1072
BseJI GATNNNNATC 2 cut(s) 47, 83
BseLI CCNNNNNNNGG 3 cut(s) 379, 441, 1269
BseMII CTCAG 2 cut(s) 609, 1038
BseNI ACTGG 2 cut(s) 702, 1110
BseRI GAGGAG 3 cut(s) 180, 441, 444
Bsh1236I CGCG 1 cut(s) 1275
Bsh1285I CGRYCG 3 cut(s) 568, 800, 828
BshFI GGCC 2 cut(s) 445, 846
BshNI GGYRCC 1 cut(s) 1316
BsiEI CGRYCG 3 cut(s) 568, 800, 828
BsiHKCI CYCGRG 1 cut(s) 531
BsiSI CCGG 5 cut(s) 393, 532, 800, 810, 828
BslI CCNNNNNNNGG 3 cut(s) 379, 441, 1269
BsmAI GTCTC 3 cut(s) 67, 1096, 1194
BsmBI CGTCTC 1 cut(s) 67
BsnI GGCC 2 cut(s) 445, 846
BsoBI CYCGRG 1 cut(s) 531
Bsp143I GATC 7 cut(s) 42, 97, 263, 336, 1182, 1372, 1418
BspANI GGCC 2 cut(s) 445, 846
BspCNI CTCAG 2 cut(s) 610, 1039
BspFNI CGCG 1 cut(s) 1275
BspLI GGNNCC 4 cut(s) 537, 814, 924, 1318
BspMI ACCTGC 1 cut(s) 1133
BspT107I GGYRCC 1 cut(s) 1316
BsrFI RCCGGY 1 cut(s) 799
BsrI ACTGG 2 cut(s) 702, 1110
BssAI RCCGGY 1 cut(s) 799
BssECI CCNNGG 4 cut(s) 440, 530, 531, 711
BssMI GATC 7 cut(s) 42, 97, 263, 336, 1182, 1372, 1418
BssNI GRCGYC 1 cut(s) 1241
BssT1I CCWWGG 1 cut(s) 711
Bst4CI ACNGT 4 cut(s) 283, 608, 940, 1302
Bst6I CTCTTC 2 cut(s) 490, 1086
BstACI GRCGYC 1 cut(s) 1241
BstBAI YACGTR 1 cut(s) 1135
BstC8I GCNNGC 2 cut(s) 911, 1012
BstDEI CTNAG 3 cut(s) 618, 693, 1047
BstDSI CCRYGG 1 cut(s) 440
BstF5I GGATG 3 cut(s) 84, 630, 1072
BstFNI CGCG 1 cut(s) 1275
BstHHI GCGC 1 cut(s) 1275
BstKTI GATC 7 cut(s) 45, 100, 266, 339, 1185, 1375, 1421
BstMAI GTCTC 3 cut(s) 67, 1096, 1194
BstMBI GATC 7 cut(s) 42, 97, 263, 336, 1182, 1372, 1418
BstMCI CGRYCG 3 cut(s) 568, 800, 828
BstMWI GCNNNNNNNGC 3 cut(s) 275, 758, 1016
BstNSI RCATGY 3 cut(s) 913, 1159, 1312
BstSCI CCNGG 4 cut(s) 530, 531, 809, 827
BstSFI CTRYAG 1 cut(s) 279
BstUI CGCG 1 cut(s) 1275
BsuRI GGCC 2 cut(s) 445, 846
BtgI CCRYGG 1 cut(s) 440
BtrI CACGTC 1 cut(s) 201
BtsCI GGATG 3 cut(s) 84, 630, 1072
BtsIMutI CAGTG 2 cut(s) 422, 709
BveI ACCTGC 1 cut(s) 1133
Cac8I GCNNGC 2 cut(s) 911, 1012
CaiI CAGNNNCTG 1 cut(s) 1046
CfoI GCGC 1 cut(s) 1275
Cfr10I RCCGGY 1 cut(s) 799
Cfr13I GGNCC 3 cut(s) 813, 844, 922
Cfr9I CCCGGG 1 cut(s) 531
CseI GACGC 2 cut(s) 453, 1249
Csp6I GTAC 5 cut(s) 4, 298, 609, 666, 788
CviAII CATG 8 cut(s) 22, 215, 408, 910, 1156, 1177, 1204, 1309
CviJI RGCY 9 cut(s) 269, 332, 399, 445, 742, 803, 846, 1262, 1406
CviKI_1 RGCY 9 cut(s) 269, 332, 399, 445, 742, 803, 846, 1262, 1406
CviQI GTAC 5 cut(s) 4, 298, 609, 666, 788
DdeI CTNAG 3 cut(s) 618, 693, 1047
DpnI GATC 7 cut(s) 44, 99, 265, 338, 1184, 1374, 1420
DpnII GATC 7 cut(s) 42, 97, 263, 336, 1182, 1372, 1418
DriI GACNNNNNGTC 1 cut(s) 894
EaeI YGGCCR 1 cut(s) 443
Eam1104I CTCTTC 2 cut(s) 490, 1086
Eam1105I GACNNNNNGTC 1 cut(s) 894
EarI CTCTTC 2 cut(s) 490, 1086
EciI GGCGGA 1 cut(s) 351
Eco130I CCWWGG 1 cut(s) 711
Eco47I GGWCC 2 cut(s) 813, 922
Eco88I CYCGRG 1 cut(s) 531
EcoRI GAATTC 1 cut(s) 856
EcoT14I CCWWGG 1 cut(s) 711
ErhI CCWWGG 1 cut(s) 711
Esp3I CGTCTC 1 cut(s) 67
FaeI CATG 8 cut(s) 25, 218, 411, 913, 1159, 1180, 1207, 1312
FatI CATG 8 cut(s) 21, 214, 407, 909, 1155, 1176, 1203, 1308
FauI CCCGC 4 cut(s) 856, 1007, 1012, 1102
FbaI TGATCA 1 cut(s) 97
Fnu4HI GCNGC 2 cut(s) 365, 740
FokI GGATG 3 cut(s) 71, 617, 1079
Fsp4HI GCNGC 2 cut(s) 365, 740
FspBI CTAG 3 cut(s) 138, 249, 1259
GlaI GCGC 1 cut(s) 1274
GluI GCNGC 2 cut(s) 365, 740
GsuI CTGGAG 1 cut(s) 1306
HaeIII GGCC 2 cut(s) 445, 846
HapII CCGG 5 cut(s) 393, 532, 800, 810, 828
HgaI GACGC 2 cut(s) 453, 1249
HhaI GCGC 1 cut(s) 1275
Hin1I GRCGYC 1 cut(s) 1241
Hin1II CATG 8 cut(s) 25, 218, 411, 913, 1159, 1180, 1207, 1312
Hin6I GCGC 1 cut(s) 1273
HinP1I GCGC 1 cut(s) 1273
HincII GTYRAC 2 cut(s) 880, 905
HindII GTYRAC 2 cut(s) 880, 905
HinfI GANTC 7 cut(s) 412, 679, 730, 867, 1037, 1278, 1354
HpaII CCGG 5 cut(s) 393, 532, 800, 810, 828
HphI GGTGA 4 cut(s) 506, 637, 1096, 1181
Hpy166II GTNNAC 3 cut(s) 880, 905, 1053
Hpy188III TCNNGA 4 cut(s) 40, 88, 258, 539
Hpy8I GTNNAC 3 cut(s) 880, 905, 1053
Hpy99I CGWCG 2 cut(s) 298, 608
HpyAV CCTTC 2 cut(s) 78, 486
HpyCH4III ACNGT 4 cut(s) 283, 608, 940, 1302
HpyCH4IV ACGT 3 cut(s) 200, 876, 1134
HpyCH4V TGCA 4 cut(s) 510, 525, 1142, 1292
HpyF10VI GCNNNNNNNGC 3 cut(s) 275, 758, 1016
HpyF3I CTNAG 3 cut(s) 618, 693, 1047
HpySE526I ACGT 3 cut(s) 200, 876, 1134
Hsp92I GRCGYC 1 cut(s) 1241
Hsp92II CATG 8 cut(s) 25, 218, 411, 913, 1159, 1180, 1207, 1312
HspAI GCGC 1 cut(s) 1273
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 7 cut(s) 42, 97, 263, 336, 1182, 1372, 1418
LmnI GCTCC 1 cut(s) 1112
LweI GCATC 1 cut(s) 997
MaeI CTAG 3 cut(s) 138, 249, 1259
MaeII ACGT 3 cut(s) 200, 876, 1134
MaeIII GTNAC 4 cut(s) 704, 766, 1130, 1237
MalI GATC 7 cut(s) 44, 99, 265, 338, 1184, 1374, 1420
MboI GATC 7 cut(s) 42, 97, 263, 336, 1182, 1372, 1418
MboII GAAGA 6 cut(s) 37, 43, 46, 273, 477, 1103
MluCI AATT 9 cut(s) 641, 856, 916, 960, 981, 1254, 1332, 1339, 1367
MlyI GAGTC 1 cut(s) 1287
MmeI TCCRAC 4 cut(s) 1024, 1176, 1276, 1329
MroXI GAANNNNTTC 1 cut(s) 1254
MseI TTAA 1 cut(s) 30
MspA1I CMGCKG 1 cut(s) 1014
MspI CCGG 5 cut(s) 393, 532, 800, 810, 828
MspR9I CCNGG 4 cut(s) 532, 533, 811, 829
MvnI CGCG 1 cut(s) 1275
MwoI GCNNNNNNNGC 3 cut(s) 275, 758, 1016
NciI CCSGG 4 cut(s) 532, 533, 811, 829
NdeII GATC 7 cut(s) 42, 97, 263, 336, 1182, 1372, 1418
NlaIII CATG 8 cut(s) 25, 218, 411, 913, 1159, 1180, 1207, 1312
NlaIV GGNNCC 4 cut(s) 537, 814, 924, 1318
NmuCI GTSAC 2 cut(s) 704, 1237
NspI RCATGY 3 cut(s) 913, 1159, 1312
PaeI GCATGC 1 cut(s) 913
PaqCI CACCTGC 1 cut(s) 1133
PciI ACATGT 1 cut(s) 1155
PdmI GAANNNNTTC 1 cut(s) 1254
PfeI GAWTC 6 cut(s) 412, 679, 730, 867, 1037, 1354
PflMI CCANNNNNTGG 1 cut(s) 379
PkrI GCNGC 2 cut(s) 366, 741
PleI GAGTC 1 cut(s) 1286
PpsI GAGTC 1 cut(s) 1286
Ppu21I YACGTR 1 cut(s) 1135
PscI ACATGT 1 cut(s) 1155
PspN4I GGNNCC 4 cut(s) 537, 814, 924, 1318
PspPI GGNCC 3 cut(s) 813, 844, 922
PstNI CAGNNNCTG 1 cut(s) 1046
RsaI GTAC 5 cut(s) 5, 299, 610, 667, 789
RsaNI GTAC 5 cut(s) 4, 298, 609, 666, 788
SaqAI TTAA 1 cut(s) 30
SatI GCNGC 2 cut(s) 365, 740
Sau3AI GATC 7 cut(s) 42, 97, 263, 336, 1182, 1372, 1418
Sau96I GGNCC 3 cut(s) 813, 844, 922
SchI GAGTC 1 cut(s) 1287
ScrFI CCNGG 4 cut(s) 532, 533, 811, 829
SfaNI GCATC 1 cut(s) 997
SfcI CTRYAG 1 cut(s) 279
SinI GGWCC 2 cut(s) 813, 922
SmaI CCCGGG 1 cut(s) 533
SpeI ACTAGT 1 cut(s) 137
SphI GCATGC 1 cut(s) 913
Sse9I AATT 9 cut(s) 641, 856, 916, 960, 981, 1254, 1332, 1339, 1367
SspMI CTAG 3 cut(s) 138, 249, 1259
StyD4I CCNGG 4 cut(s) 530, 531, 809, 827
StyI CCWWGG 1 cut(s) 711
TaaI ACNGT 4 cut(s) 283, 608, 940, 1302
TaiI ACGT 3 cut(s) 203, 879, 1137
TaqI TCGA 2 cut(s) 77, 805
TaqII GACCGA 1 cut(s) 582
TasI AATT 9 cut(s) 641, 856, 916, 960, 981, 1254, 1332, 1339, 1367
TauI GCSGC 2 cut(s) 367, 742
TfiI GAWTC 6 cut(s) 412, 679, 730, 867, 1037, 1354
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TscAI CASTG 2 cut(s) 422, 709
TseFI GTSAC 2 cut(s) 704, 1237
Tsp45I GTSAC 2 cut(s) 704, 1237
TspDTI ATGAA 4 cut(s) 37, 743, 1083, 1334
TspGWI ACGGA 3 cut(s) 282, 649, 1271
TspMI CCCGGG 1 cut(s) 531
TspRI CASTG 2 cut(s) 422, 709
Van91I CCANNNNNTGG 1 cut(s) 379
VpaK11BI GGWCC 2 cut(s) 813, 922
XapI RAATTY 7 cut(s) 641, 856, 916, 981, 1254, 1339, 1367
XceI RCATGY 3 cut(s) 913, 1159, 1312
XcmI CCANNNNNNNNNTGG 1 cut(s) 718
XmaI CCCGGG 1 cut(s) 531
XmnI GAANNNNTTC 1 cut(s) 1254
XspI CTAG 3 cut(s) 138, 249, 1259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.