MD09G1285600.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006672
Physical Location & Seq
Forward (+)
36386025 .. 36390734
4710 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1285600.v1.1.491

Sequence Viewer

Length: 1401 bp
ATGGAAACGAGACCGGACCCAGACATCGATGATGACTTTAGCGAACTATACAAGGAGTACACTGGCCCTCCTGGGTCAAATGCCACAAACACACAAGATAGGGCAAAACCAAACAAAAGGTCTCTTGCCGGGTCTGATGAGGAAGAGGAAGCTCGTGACCCCAACGCTGTCCCAACCGATTTTACCAGCCGAGAAGCTAAGGTGTGGGAGGCCAAGTCAAAAGCTACTGAGAGGAATTGGAAGAAGAGGAAGGAAGAGGAAATGATTTGCAAAATCTGCGGGGAACCAGGTCACTTTACTCAGGGTTGCCCATCTACCCTTGGTGCAAGTCGCAAGTCTCAAGATTTCTTTGAAAGGGTTCCTGCTAGAGAAAAGCATGTAAGGTCCCTTTTCACTGAGAAAGTGATAAACAGGATTGAAATGGATATTGGATGCTATATTAAGATGGACGAGAAATTTATCATTGTCGGTGGCAAGGATAGGTTAGTATTAAGAAGGGGTGTGGATGCTGTGCATAAAGTGATTAAAGAGGAAGGCGAACAAAGGGATGGTTCTGGTTCTCAAGTGACTAGATCAAGGTCACCTGAACGAAGCCCTGTTGTTGCACGGTTACAACGTTCTGAATCCCAGAGGTCTCATTCTGGTCCCCGGAACGGGTCGCATTTTCAACAAAGGTTTCACAGGAAAGATAAATTTGTTGAAGACCGAATACGTCATGAGATGCAGAAATTTTCCAGGGGTCCTCCACAAGCTTATGGTAATAACGGAGCTAGAGGTCGGCCAAGTCGTTCAAATTCTCCAGCTTGTGCCCCTTACATGGGAAACTCATATAATTCATATGATGGTCATAATCAAAGCATGTCTGCTTATAGAAATGATGGATGGAATTCTGATAAAAAAGGATCTGATATGCAATCTGGTCGTCAGTTTGATTACTCTGCCATCCCCCAGACATTGGAAGAATTAGAAATGGAGTACAAACGAGAGGCAATGGAACTTGGAAGAATTCATGACAAGGAAGAAGATGAAGAAAATTACAGACATCGTGAGGCGATTAGGGAAATAAGAGAGAGCCACATGAAGAAACTGCCCCCCCTGAGGGGCATGCATGCAAAGCAGTGGGAGGACTTTCTTCAACTTGATGCCCAGAGGCGTCAACAACAGGCTAATCAGCAGATGTCGACTTTGGGTTTTGGTGGTTATAAATCACACAATTATTCTGAATACGACGGCACCTCGGCCAATCCTCAGTATGCTGGGGAAAGTATGGCCATGGATTCAAGGAGCAGGTACCCCAATCCCAACCCTATGGAAACCTATCCTTCAAGGCCTCGTGACAATTTTGGTGAGTTTCAGCGTCAGAGGCACGAGGGCTTTGGGAGAGGTTACAATCGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

467

Amino Acids

53.92

Weight (kDa)

8.71

Isoelectric Point (pI)

61.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1203
Acc36I ACCTGC 1 cut(s) 1278
Acc65I GGTACC 1 cut(s) 1290
AccB1I GGYRCC 2 cut(s) 1232, 1290
AccB7I CCANNNNNTGG 1 cut(s) 955
AccI GTMKAC 1 cut(s) 1181
AciI CCGC 1 cut(s) 279
AclI AACGTT 1 cut(s) 616
AclWI GGATC 1 cut(s) 910
AcoI YGGCCR 3 cut(s) 779, 1239, 1269
AcsI RAATTY 6 cut(s) 455, 692, 728, 793, 886, 1005
AcyI GRCGYC 1 cut(s) 1153
AfaI GTAC 3 cut(s) 59, 977, 1292
AfiI CCNNNNNNNGG 8 cut(s) 653, 654, 817, 818, 955, 1098, 1099, 1100
AgsI TTSAA 8 cut(s) 353, 419, 668, 701, 792, 1136, 1281, 1326
AjnI CCWGG 3 cut(s) 70, 286, 734
AjuI GAANNNNNNNTTGG 2 cut(s) 411, 443
AluBI AGCT 6 cut(s) 152, 197, 224, 752, 770, 803
AluI AGCT 6 cut(s) 152, 197, 224, 752, 770, 803
Alw26I GTCTC 4 cut(s) 4, 126, 342, 639
AlwI GGATC 1 cut(s) 910
AoxI GGCC 6 cut(s) 64, 210, 779, 1239, 1269, 1328
ApoI RAATTY 6 cut(s) 455, 692, 728, 793, 886, 1005
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
Asp700I GAANNNNTTC 1 cut(s) 357
Asp718I GGTACC 1 cut(s) 1290
AspS9I GGNCC 5 cut(s) 16, 65, 384, 644, 740
AsuC2I CCSGG 2 cut(s) 130, 649
AsuHPI GGTGA 2 cut(s) 573, 1358
AvaII GGWCC 4 cut(s) 16, 384, 644, 740
AxyI CCTNAGG 1 cut(s) 1097
BaeGI GKGCMC 1 cut(s) 811
BalI TGGCCA 1 cut(s) 1271
BanI GGYRCC 2 cut(s) 1232, 1290
BarI GAAGNNNNNNTAC 2 cut(s) 693, 725
BauI CACGAG 3 cut(s) 153, 1332, 1367
BbsI GAAGAC 1 cut(s) 708
BccI CCATC 7 cut(s) 319, 439, 542, 836, 872, 876, 950
BceAI ACGGC 1 cut(s) 1246
BcgI CGANNNNNNTGC 2 cut(s) 902, 936
BciT130I CCWGG 3 cut(s) 72, 288, 736
BcnI CCSGG 2 cut(s) 130, 649
BcoDI GTCTC 4 cut(s) 4, 126, 342, 639
BfaI CTAG 3 cut(s) 366, 570, 771
BfuAI ACCTGC 1 cut(s) 1278
Bme1390I CCNGG 5 cut(s) 72, 130, 288, 649, 736
Bme18I GGWCC 4 cut(s) 16, 384, 644, 740
BmgT120I GGNCC 5 cut(s) 16, 65, 384, 644, 740
BmiI GGNNCC 8 cut(s) 18, 285, 360, 386, 646, 741, 1234, 1292
BmrFI CCNGG 5 cut(s) 72, 130, 288, 649, 736
BmsI GCATC 4 cut(s) 422, 496, 711, 1132
BpiI GAAGAC 1 cut(s) 708
BpmI CTGGAG 1 cut(s) 783
Bpu10I CCTNAGC 1 cut(s) 198
BpuEI CTTGAG 2 cut(s) 324, 546
BpuMI CCSGG 2 cut(s) 130, 649
Bsa29I ATCGAT 2 cut(s) 27, 1393
BsaHI GRCGYC 1 cut(s) 1153
BsaI GGTCTC 3 cut(s) 4, 126, 639
BsaJI CCNNGG 6 cut(s) 71, 319, 647, 735, 1236, 1272
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 4 cut(s) 52, 82, 200, 230
Bsc4I CCNNNNNNNGG 8 cut(s) 653, 654, 817, 818, 955, 1098, 1099, 1100
Bse1I ACTGG 1 cut(s) 67
Bse21I CCTNAGG 1 cut(s) 1097
Bse3DI GCAATG 1 cut(s) 996
BseBI CCWGG 3 cut(s) 72, 288, 736
BseCI ATCGAT 2 cut(s) 27, 1393
BseDI CCNNGG 6 cut(s) 71, 319, 647, 735, 1236, 1272
BseGI GGATG 5 cut(s) 437, 511, 553, 887, 942
BseLI CCNNNNNNNGG 8 cut(s) 653, 654, 817, 818, 955, 1098, 1099, 1100
BseMI GCAATG 1 cut(s) 996
BseMII CTCAG 5 cut(s) 219, 314, 387, 1088, 1262
BseNI ACTGG 1 cut(s) 67
BseSI GKGCMC 1 cut(s) 811
BseYI CCCAGC 1 cut(s) 1256
BshFI GGCC 6 cut(s) 66, 212, 781, 1241, 1271, 1330
BshNI GGYRCC 2 cut(s) 1232, 1290
BshVI ATCGAT 2 cut(s) 27, 1393
BsiSI CCGG 3 cut(s) 14, 129, 649
BslFI GGGAC 3 cut(s) 155, 370, 630
BslI CCNNNNNNNGG 8 cut(s) 653, 654, 817, 818, 955, 1098, 1099, 1100
BsmAI GTCTC 4 cut(s) 4, 126, 342, 639
BsmFI GGGAC 3 cut(s) 155, 370, 630
BsnI GGCC 6 cut(s) 66, 212, 781, 1241, 1271, 1330
Bso31I GGTCTC 3 cut(s) 4, 126, 639
Bsp1286I GDGCHC 1 cut(s) 811
Bsp143I GATC 2 cut(s) 572, 902
Bsp19I CCATGG 1 cut(s) 1272
BspACI CCGC 1 cut(s) 279
BspANI GGCC 6 cut(s) 66, 212, 781, 1241, 1271, 1330
BspCNI CTCAG 5 cut(s) 220, 313, 388, 1089, 1261
BspDI ATCGAT 2 cut(s) 27, 1393
BspHI TCATGA 2 cut(s) 715, 1009
BspLI GGNNCC 8 cut(s) 18, 285, 360, 386, 646, 741, 1234, 1292
BspMI ACCTGC 1 cut(s) 1278
BspPI GGATC 1 cut(s) 910
BspT107I GGYRCC 2 cut(s) 1232, 1290
BspTNI GGTCTC 3 cut(s) 4, 126, 639
BsrDI GCAATG 1 cut(s) 996
BsrI ACTGG 1 cut(s) 67
BssECI CCNNGG 6 cut(s) 71, 319, 647, 735, 1236, 1272
BssMI GATC 2 cut(s) 572, 902
BssNI GRCGYC 1 cut(s) 1153
BssSI CACGAG 3 cut(s) 153, 1332, 1367
BssT1I CCWWGG 2 cut(s) 319, 1272
Bst2BI CACGAG 3 cut(s) 153, 1332, 1367
Bst2UI CCWGG 3 cut(s) 72, 288, 736
Bst4CI ACNGT 1 cut(s) 609
Bst6I CTCTTC 3 cut(s) 138, 239, 249
BstACI GRCGYC 1 cut(s) 1153
BstAPI GCANNNNNTGC 1 cut(s) 276
BstC8I GCNNGC 2 cut(s) 1106, 1110
BstDEI CTNAG 6 cut(s) 198, 228, 300, 396, 1097, 1248
BstDSI CCRYGG 1 cut(s) 1272
BstEII GGTNACC 1 cut(s) 579
BstF5I GGATG 5 cut(s) 437, 511, 553, 887, 942
BstKTI GATC 2 cut(s) 575, 905
BstMAI GTCTC 4 cut(s) 4, 126, 342, 639
BstMBI GATC 2 cut(s) 572, 902
BstMWI GCNNNNNNNGC 3 cut(s) 276, 1114, 1363
BstNI CCWGG 3 cut(s) 72, 288, 736
BstNSI RCATGY 4 cut(s) 380, 862, 1108, 1112
BstPI GGTNACC 1 cut(s) 579
BstSCI CCNGG 5 cut(s) 70, 128, 286, 647, 734
BstSLI GKGCMC 1 cut(s) 811
BstV2I GAAGAC 1 cut(s) 708
BstX2I RGATCY 1 cut(s) 902
BstXI CCANNNNNNTGG 1 cut(s) 1309
BstYI RGATCY 1 cut(s) 902
Bsu15I ATCGAT 2 cut(s) 27, 1393
Bsu36I CCTNAGG 1 cut(s) 1097
BsuRI GGCC 6 cut(s) 66, 212, 781, 1241, 1271, 1330
BsuTUI ATCGAT 2 cut(s) 27, 1393
BtgI CCRYGG 1 cut(s) 1272
BtsCI GGATG 5 cut(s) 437, 511, 553, 887, 942
BtsI GCAGTG 1 cut(s) 1124
BtsIMutI CAGTG 3 cut(s) 60, 393, 1124
BveI ACCTGC 1 cut(s) 1278
Cac8I GCNNGC 2 cut(s) 1106, 1110
CciI TCATGA 2 cut(s) 715, 1009
Cfr13I GGNCC 5 cut(s) 16, 65, 384, 644, 740
ClaI ATCGAT 2 cut(s) 27, 1393
CseI GACGC 2 cut(s) 1142, 1346
CsiI ACCWGGT 1 cut(s) 286
Csp6I GTAC 3 cut(s) 58, 976, 1291
CviAII CATG 9 cut(s) 377, 716, 817, 859, 1010, 1078, 1105, 1109, 1273
CviQI GTAC 3 cut(s) 58, 976, 1291
DdeI CTNAG 6 cut(s) 198, 228, 300, 396, 1097, 1248
DpnI GATC 2 cut(s) 574, 904
DpnII GATC 2 cut(s) 572, 902
EaeI YGGCCR 3 cut(s) 779, 1239, 1269
Eam1104I CTCTTC 3 cut(s) 138, 239, 249
EarI CTCTTC 3 cut(s) 138, 239, 249
Eco130I CCWWGG 2 cut(s) 319, 1272
Eco147I AGGCCT 1 cut(s) 1330
Eco31I GGTCTC 3 cut(s) 4, 126, 639
Eco47I GGWCC 4 cut(s) 16, 384, 644, 740
Eco81I CCTNAGG 1 cut(s) 1097
Eco91I GGTNACC 1 cut(s) 579
EcoO109I RGGNCCY 2 cut(s) 384, 740
EcoO65I GGTNACC 1 cut(s) 579
EcoRI GAATTC 2 cut(s) 886, 1005
EcoRII CCWGG 3 cut(s) 70, 286, 734
EcoT14I CCWWGG 2 cut(s) 319, 1272
EcoT22I ATGCAT 1 cut(s) 1110
ErhI CCWWGG 2 cut(s) 319, 1272
FaeI CATG 9 cut(s) 380, 719, 820, 862, 1013, 1081, 1108, 1112, 1276
FaqI GGGAC 3 cut(s) 155, 370, 630
FatI CATG 9 cut(s) 376, 715, 816, 858, 1009, 1077, 1104, 1108, 1272
FauI CCCGC 1 cut(s) 272
FauNDI CATATG 1 cut(s) 838
FblI GTMKAC 1 cut(s) 1181
FokI GGATG 5 cut(s) 444, 518, 560, 894, 929
FspBI CTAG 3 cut(s) 366, 570, 771
GsaI CCCAGC 1 cut(s) 1260
GsuI CTGGAG 1 cut(s) 783
HaeIII GGCC 6 cut(s) 66, 212, 781, 1241, 1271, 1330
HapII CCGG 3 cut(s) 14, 129, 649
HgaI GACGC 2 cut(s) 1142, 1346
Hin1I GRCGYC 1 cut(s) 1153
Hin1II CATG 9 cut(s) 380, 719, 820, 862, 1013, 1081, 1108, 1112, 1276
HincII GTYRAC 2 cut(s) 1157, 1182
HindII GTYRAC 2 cut(s) 1157, 1182
HindIII AAGCTT 1 cut(s) 750
HinfI GANTC 2 cut(s) 623, 1277
HpaII CCGG 3 cut(s) 14, 129, 649
HphI GGTGA 2 cut(s) 573, 1358
Hpy166II GTNNAC 3 cut(s) 60, 1157, 1182
Hpy188I TCNGA 6 cut(s) 136, 622, 892, 907, 1222, 1362
Hpy188III TCNNGA 6 cut(s) 155, 341, 716, 1010, 1046, 1334
Hpy8I GTNNAC 3 cut(s) 60, 1157, 1182
Hpy99I CGWCG 1 cut(s) 1232
HpyAV CCTTC 4 cut(s) 244, 489, 527, 1332
HpyCH4III ACNGT 1 cut(s) 609
HpyCH4IV ACGT 2 cut(s) 616, 712
HpyCH4V TGCA 8 cut(s) 270, 326, 514, 605, 724, 913, 1108, 1112
HpyF10VI GCNNNNNNNGC 3 cut(s) 276, 1114, 1363
HpyF3I CTNAG 6 cut(s) 198, 228, 300, 396, 1097, 1248
HpySE526I ACGT 2 cut(s) 616, 712
Hsp92I GRCGYC 1 cut(s) 1153
Hsp92II CATG 9 cut(s) 380, 719, 820, 862, 1013, 1081, 1108, 1112, 1276
KpnI GGTACC 1 cut(s) 1294
Kzo9I GATC 2 cut(s) 572, 902
LmnI GCTCC 2 cut(s) 767, 1284
LweI GCATC 4 cut(s) 422, 496, 711, 1132
MabI ACCWGGT 1 cut(s) 286
MaeI CTAG 3 cut(s) 366, 570, 771
MaeII ACGT 2 cut(s) 616, 712
MaeIII GTNAC 7 cut(s) 155, 290, 565, 579, 609, 1334, 1385
MalI GATC 2 cut(s) 574, 904
MboI GATC 2 cut(s) 572, 902
MflI RGATCY 1 cut(s) 902
MhlI GDGCHC 1 cut(s) 811
MlsI TGGCCA 1 cut(s) 1271
MluNI TGGCCA 1 cut(s) 1271
Mox20I TGGCCA 1 cut(s) 1271
Mph1103I ATGCAT 1 cut(s) 1110
MroXI GAANNNNTTC 1 cut(s) 357
MscI TGGCCA 1 cut(s) 1271
MseI TTAA 3 cut(s) 441, 491, 525
Msp20I TGGCCA 1 cut(s) 1271
MspI CCGG 3 cut(s) 14, 129, 649
MspR9I CCNGG 5 cut(s) 72, 130, 288, 649, 736
MvaI CCWGG 3 cut(s) 72, 288, 736
MwoI GCNNNNNNNGC 3 cut(s) 276, 1114, 1363
NciI CCSGG 2 cut(s) 130, 649
NcoI CCATGG 1 cut(s) 1272
NdeI CATATG 1 cut(s) 838
NdeII GATC 2 cut(s) 572, 902
NlaIII CATG 9 cut(s) 380, 719, 820, 862, 1013, 1081, 1108, 1112, 1276
NlaIV GGNNCC 8 cut(s) 18, 285, 360, 386, 646, 741, 1234, 1292
NmeAIII GCCGAG 2 cut(s) 215, 1217
NmuCI GTSAC 5 cut(s) 155, 290, 565, 579, 1334
NsiI ATGCAT 1 cut(s) 1110
NspI RCATGY 4 cut(s) 380, 862, 1108, 1112
PaeI GCATGC 2 cut(s) 1108, 1112
PagI TCATGA 2 cut(s) 715, 1009
PceI AGGCCT 1 cut(s) 1330
PcsI WCGNNNNNNNCGW 2 cut(s) 613, 784
PdmI GAANNNNTTC 1 cut(s) 357
PfeI GAWTC 2 cut(s) 623, 1277
PflMI CCANNNNNTGG 1 cut(s) 955
PpuMI RGGWCCY 2 cut(s) 384, 740
PsiI TTATAA 1 cut(s) 1203
Psp1406I AACGTT 1 cut(s) 616
Psp5II RGGWCCY 2 cut(s) 384, 740
Psp6I CCWGG 3 cut(s) 70, 286, 734
PspEI GGTNACC 1 cut(s) 579
PspFI CCCAGC 1 cut(s) 1256
PspGI CCWGG 3 cut(s) 70, 286, 734
PspN4I GGNNCC 8 cut(s) 18, 285, 360, 386, 646, 741, 1234, 1292
PspPI GGNCC 5 cut(s) 16, 65, 384, 644, 740
PspPPI RGGWCCY 2 cut(s) 384, 740
PsuI RGATCY 1 cut(s) 902
RsaI GTAC 3 cut(s) 59, 977, 1292
RsaNI GTAC 3 cut(s) 58, 976, 1291
SalI GTCGAC 1 cut(s) 1180
SaqAI TTAA 3 cut(s) 441, 491, 525
Sau3AI GATC 2 cut(s) 572, 902
Sau96I GGNCC 5 cut(s) 16, 65, 384, 644, 740
ScrFI CCNGG 5 cut(s) 72, 130, 288, 649, 736
SduI GDGCHC 1 cut(s) 811
SexAI ACCWGGT 1 cut(s) 286
SfaNI GCATC 4 cut(s) 422, 496, 711, 1132
SinI GGWCC 4 cut(s) 16, 384, 644, 740
SmlI CTYRAG 2 cut(s) 339, 561
SmoI CTYRAG 2 cut(s) 339, 561
SphI GCATGC 2 cut(s) 1108, 1112
SseBI AGGCCT 1 cut(s) 1330
SsiI CCGC 1 cut(s) 279
SspMI CTAG 3 cut(s) 366, 570, 771
StuI AGGCCT 1 cut(s) 1330
StyD4I CCNGG 5 cut(s) 70, 128, 286, 647, 734
StyI CCWWGG 2 cut(s) 319, 1272
TaaI ACNGT 1 cut(s) 609
TaiI ACGT 2 cut(s) 619, 715
TaqI TCGA 3 cut(s) 27, 1181, 1393
TaqII GACCGA 1 cut(s) 720
TatI WGTACW 2 cut(s) 57, 975
TfiI GAWTC 2 cut(s) 623, 1277
Tru1I TTAA 3 cut(s) 441, 491, 525
Tru9I TTAA 3 cut(s) 441, 491, 525
TscAI CASTG 3 cut(s) 67, 400, 1124
TseFI GTSAC 5 cut(s) 155, 290, 565, 579, 1334
Tsp45I GTSAC 5 cut(s) 155, 290, 565, 579, 1334
TspDTI ATGAA 4 cut(s) 825, 998, 1041, 1094
TspGWI ACGGA 1 cut(s) 780
TspRI CASTG 3 cut(s) 67, 400, 1124
Van91I CCANNNNNTGG 1 cut(s) 955
VpaK11BI GGWCC 4 cut(s) 16, 384, 644, 740
XapI RAATTY 6 cut(s) 455, 692, 728, 793, 886, 1005
XceI RCATGY 4 cut(s) 380, 862, 1108, 1112
XmiI GTMKAC 1 cut(s) 1181
XmnI GAANNNNTTC 1 cut(s) 357
XspI CTAG 3 cut(s) 366, 570, 771
Zsp2I ATGCAT 1 cut(s) 1110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.