Rmu_ssc0000366.1_g000037

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000366.1
OG0006672
Physical Location & Seq
Forward (+)
151295 .. 154408
3114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000366.1_g000037.1.cds

Sequence Viewer

Length: 1248 bp
atggaacctaaaccagacccggatattgatgatgattttagcgagctgtacaaggagtacactggccctcctggctcaaatgccacaaacacacaagacagagcaaaaccgaataagaggtcccttgctgggtctgatgaggaagaggaagctcgcgacccgaatgctgtcccgactgatttcactagccgggaagctaaggtttgggaggcaaagtcaaaagctactgagaggaattggaagaagaggaaggaagaggaactgatttgcaaaatatgcggagaatcggggcactttacgcagatggacgagaaattcatcattgtcagtggcaaagataggttaatattaaggaaaggcgtggatgctgtgcacaagatgatgagagaggaaggtgaacaacggggtagttctcaagtgactagatcaaggtcacccgagcgaagccctgtaggttcacggttacaccgctctgaatcccagaggtctcattctggtcctcgtaatggatcaaactttcaaccaaggtttcacaggcaggagaagtttgttgaagaccgtatacgtcaaaatatgcaaaaaatttccaggggttctccacaagcttatggtaatgatggagctcgaggtcggccaagtcattcaaattctccagcacatgccccttacatgggcaactcatataattcatatgatggtcataatcagagcatggccacttacagaaatgatggatgggaatctgataaaaagggatctgaagtgcaatctggtcatcagtttgattacccaaccgtcccacagacattagaagaattagaattggagtacaaaagagaggcgatggagcttggaagaattcgtgacaaagaagaagatgaagaaaattacaaacatcgtgaggccattagggaaatgcgagagaactacatgaagaaactggccaccttgaggggcatgcatgcaaaacagtgggagaattttcttcagcttgatgcccagagacgtcaacaacaggctcatcagcaaatgcccactgcaggttttggtagttacaaatcacacaattacgctgaatatgatggcccagcagctaatcctcattattccgggcctactttagcaatggattcaaggagcaggtacccacacgctatggaaaattatccttcaagacctcatgacaattttggtgagttccagcgtcagagacatgaggattatgggagaggttacaatcgatactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

415

Amino Acids

48.15

Weight (kDa)

7.32

Isoelectric Point (pI)

59.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1009
Acc36I ACCTGC 2 cut(s) 1031, 1131
Acc65I GGTACC 1 cut(s) 1143
AccB1I GGYRCC 1 cut(s) 1143
AccBSI CCGCTC 1 cut(s) 471
AccI GTMKAC 1 cut(s) 562
AccII CGCG 1 cut(s) 156
AciI CCGC 2 cut(s) 279, 469
AclWI GGATC 2 cut(s) 517, 763
AcoI YGGCCR 3 cut(s) 632, 714, 942
AcsI RAATTY 5 cut(s) 314, 582, 646, 858, 979
AcuI CTGAAG 2 cut(s) 780, 971
AcyI GRCGYC 1 cut(s) 1006
AfaI GTAC 4 cut(s) 50, 59, 830, 1145
AfiI CCNNNNNNNGG 6 cut(s) 129, 506, 670, 671, 951, 1040
AgsI TTSAA 5 cut(s) 521, 554, 645, 1134, 1173
AjnI CCWGG 2 cut(s) 70, 587
AluBI AGCT 9 cut(s) 46, 152, 197, 224, 605, 623, 850, 991, 1094
AluI AGCT 9 cut(s) 46, 152, 197, 224, 605, 623, 850, 991, 1094
Alw21I GWGCWC 2 cut(s) 375, 625
Alw26I GTCTC 3 cut(s) 492, 997, 1204
Alw44I GTGCAC 1 cut(s) 371
AlwI GGATC 2 cut(s) 517, 763
Ama87I CYCGRG 2 cut(s) 437, 624
AoxI GGCC 7 cut(s) 64, 632, 714, 903, 942, 1084, 1112
ApaLI GTGCAC 1 cut(s) 371
ApeKI GCWGC 1 cut(s) 1091
ApoI RAATTY 5 cut(s) 314, 582, 646, 858, 979
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
Asp718I GGTACC 1 cut(s) 1143
AspS9I GGNCC 5 cut(s) 65, 120, 497, 1085, 1112
AsuC2I CCSGG 3 cut(s) 20, 191, 1111
AsuHPI GGTGA 3 cut(s) 407, 426, 1205
AvaI CYCGRG 2 cut(s) 437, 624
AvaII GGWCC 2 cut(s) 120, 497
BaeGI GKGCMC 2 cut(s) 294, 375
BalI TGGCCA 2 cut(s) 716, 944
BanI GGYRCC 1 cut(s) 1143
BanII GRGCYC 1 cut(s) 625
BarI GAAGNNNNNNTAC 2 cut(s) 546, 578
BbsI GAAGAC 1 cut(s) 561
Bbv12I GWGCWC 2 cut(s) 375, 625
BbvI GCAGC 1 cut(s) 1103
BccI CCATC 7 cut(s) 298, 611, 689, 725, 729, 838, 1076
BcgI CGANNNNNNTGC 2 cut(s) 146, 180
BciT130I CCWGG 2 cut(s) 72, 589
BcnI CCSGG 3 cut(s) 20, 191, 1111
BcoDI GTCTC 3 cut(s) 492, 997, 1204
BfaI CTAG 2 cut(s) 186, 423
BfmI CTRYAG 2 cut(s) 450, 1038
BfuAI ACCTGC 2 cut(s) 1031, 1131
BglI GCCNNNNNGGC 1 cut(s) 72
BisI GCNGC 1 cut(s) 1092
BlsI GCNGC 1 cut(s) 1093
Bme1390I CCNGG 5 cut(s) 20, 72, 191, 589, 1111
Bme18I GGWCC 2 cut(s) 120, 497
BmeT110I CYCGRG 2 cut(s) 437, 624
BmgT120I GGNCC 5 cut(s) 65, 120, 497, 1085, 1112
BmiI GGNNCC 3 cut(s) 6, 122, 1145
BmrFI CCNGG 5 cut(s) 20, 72, 191, 589, 1111
BmsI GCATC 2 cut(s) 355, 985
BpiI GAAGAC 1 cut(s) 561
BpmI CTGGAG 1 cut(s) 636
Bpu10I CCTNAGC 1 cut(s) 198
BpuEI CTTGAG 2 cut(s) 399, 970
BpuMI CCSGG 3 cut(s) 20, 191, 1111
Bsa29I ATCGAT 1 cut(s) 1240
BsaBI GATNNNNATC 1 cut(s) 739
BsaHI GRCGYC 1 cut(s) 1006
BsaI GGTCTC 1 cut(s) 492
BsaJI CCNNGG 2 cut(s) 524, 588
BsaXI ACNNNNNCTCC 4 cut(s) 52, 82, 200, 230
Bsc4I CCNNNNNNNGG 6 cut(s) 129, 506, 670, 671, 951, 1040
Bse1I ACTGG 2 cut(s) 67, 945
Bse3DI GCAATG 1 cut(s) 1131
Bse8I GATNNNNATC 1 cut(s) 739
BseBI CCWGG 2 cut(s) 72, 589
BseCI ATCGAT 1 cut(s) 1240
BseDI CCNNGG 2 cut(s) 524, 588
BseGI GGATG 2 cut(s) 370, 740
BseJI GATNNNNATC 1 cut(s) 739
BseLI CCNNNNNNNGG 6 cut(s) 129, 506, 670, 671, 951, 1040
BseMI GCAATG 1 cut(s) 1131
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 2 cut(s) 67, 945
BseSI GKGCMC 2 cut(s) 294, 375
BseXI GCAGC 1 cut(s) 1103
BseYI CCCAGC 2 cut(s) 128, 1087
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 7 cut(s) 66, 634, 716, 905, 944, 1086, 1114
BshNI GGYRCC 1 cut(s) 1143
BshVI ATCGAT 1 cut(s) 1240
BsiHKAI GWGCWC 2 cut(s) 375, 625
BsiHKCI CYCGRG 2 cut(s) 437, 624
BsiSI CCGG 3 cut(s) 20, 190, 1110
BslFI GGGAC 3 cut(s) 106, 155, 782
BslI CCNNNNNNNGG 6 cut(s) 129, 506, 670, 671, 951, 1040
BsmAI GTCTC 3 cut(s) 492, 997, 1204
BsmBI CGTCTC 1 cut(s) 997
BsmFI GGGAC 3 cut(s) 106, 155, 782
BsmI GAATGC 1 cut(s) 169
BsnI GGCC 7 cut(s) 66, 634, 716, 905, 944, 1086, 1114
Bso31I GGTCTC 1 cut(s) 492
BsoBI CYCGRG 2 cut(s) 437, 624
Bsp1286I GDGCHC 3 cut(s) 294, 375, 625
Bsp1407I TGTACA 1 cut(s) 48
Bsp143I GATC 3 cut(s) 425, 509, 755
Bsp68I TCGCGA 1 cut(s) 156
BspACI CCGC 2 cut(s) 279, 469
BspANI GGCC 7 cut(s) 66, 634, 716, 905, 944, 1086, 1114
BspCNI CTCAG 1 cut(s) 220
BspDI ATCGAT 1 cut(s) 1240
BspFNI CGCG 1 cut(s) 156
BspHI TCATGA 1 cut(s) 1180
BspLI GGNNCC 3 cut(s) 6, 122, 1145
BspMAI CTGCAG 1 cut(s) 1042
BspMI ACCTGC 2 cut(s) 1031, 1131
BspPI GGATC 2 cut(s) 517, 763
BspT107I GGYRCC 1 cut(s) 1143
BspTNI GGTCTC 1 cut(s) 492
BsrBI CCGCTC 1 cut(s) 471
BsrDI GCAATG 1 cut(s) 1131
BsrGI TGTACA 1 cut(s) 48
BsrI ACTGG 2 cut(s) 67, 945
BssECI CCNNGG 2 cut(s) 524, 588
BssMI GATC 3 cut(s) 425, 509, 755
BssNAI GTATAC 1 cut(s) 563
BssNI GRCGYC 1 cut(s) 1006
BssT1I CCWWGG 1 cut(s) 524
Bst1107I GTATAC 1 cut(s) 563
Bst2UI CCWGG 2 cut(s) 72, 589
Bst4CI ACNGT 4 cut(s) 462, 560, 796, 972
Bst6I CTCTTC 3 cut(s) 138, 239, 249
BstACI GRCGYC 1 cut(s) 1006
BstAPI GCANNNNNTGC 1 cut(s) 276
BstAUI TGTACA 1 cut(s) 48
BstC8I GCNNGC 4 cut(s) 44, 154, 959, 963
BstDEI CTNAG 2 cut(s) 198, 228
BstEII GGTNACC 1 cut(s) 432
BstF5I GGATG 2 cut(s) 370, 740
BstFNI CGCG 1 cut(s) 156
BstKTI GATC 3 cut(s) 428, 512, 758
BstMAI GTCTC 3 cut(s) 492, 997, 1204
BstMBI GATC 3 cut(s) 425, 509, 755
BstMWI GCNNNNNNNGC 3 cut(s) 72, 276, 298
BstNI CCWGG 2 cut(s) 72, 589
BstNSI RCATGY 3 cut(s) 662, 961, 965
BstPI GGTNACC 1 cut(s) 432
BstSCI CCNGG 5 cut(s) 18, 70, 189, 587, 1109
BstSFI CTRYAG 2 cut(s) 450, 1038
BstSLI GKGCMC 2 cut(s) 294, 375
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 1 cut(s) 1103
BstV2I GAAGAC 1 cut(s) 561
BstX2I RGATCY 1 cut(s) 755
BstYI RGATCY 1 cut(s) 755
BstZ17I GTATAC 1 cut(s) 563
Bsu15I ATCGAT 1 cut(s) 1240
BsuRI GGCC 7 cut(s) 66, 634, 716, 905, 944, 1086, 1114
BsuTUI ATCGAT 1 cut(s) 1240
BtgZI GCGATG 1 cut(s) 857
BtsCI GGATG 2 cut(s) 370, 740
BtsI GCAGTG 1 cut(s) 1035
BtsIMutI CAGTG 4 cut(s) 60, 334, 977, 1035
BtuMI TCGCGA 1 cut(s) 156
BveI ACCTGC 2 cut(s) 1031, 1131
Cac8I GCNNGC 4 cut(s) 44, 154, 959, 963
CciI TCATGA 1 cut(s) 1180
Cfr13I GGNCC 5 cut(s) 65, 120, 497, 1085, 1112
ClaI ATCGAT 1 cut(s) 1240
CseI GACGC 1 cut(s) 1193
Csp6I GTAC 4 cut(s) 49, 58, 829, 1144
CviAII CATG 8 cut(s) 659, 670, 712, 931, 958, 962, 1181, 1214
CviQI GTAC 4 cut(s) 49, 58, 829, 1144
DdeI CTNAG 2 cut(s) 198, 228
DpnI GATC 3 cut(s) 427, 511, 757
DpnII GATC 3 cut(s) 425, 509, 755
EaeI YGGCCR 3 cut(s) 632, 714, 942
Eam1104I CTCTTC 3 cut(s) 138, 239, 249
EarI CTCTTC 3 cut(s) 138, 239, 249
Ecl136II GAGCTC 1 cut(s) 623
Eco130I CCWWGG 1 cut(s) 524
Eco24I GRGCYC 1 cut(s) 625
Eco31I GGTCTC 1 cut(s) 492
Eco47I GGWCC 2 cut(s) 120, 497
Eco53kI GAGCTC 1 cut(s) 623
Eco57I CTGAAG 2 cut(s) 780, 971
Eco88I CYCGRG 2 cut(s) 437, 624
Eco91I GGTNACC 1 cut(s) 432
EcoICRI GAGCTC 1 cut(s) 623
EcoO109I RGGNCCY 1 cut(s) 120
EcoO65I GGTNACC 1 cut(s) 432
EcoRI GAATTC 1 cut(s) 858
EcoRII CCWGG 2 cut(s) 70, 587
EcoT14I CCWWGG 1 cut(s) 524
EcoT22I ATGCAT 1 cut(s) 963
EcoT38I GRGCYC 1 cut(s) 625
ErhI CCWWGG 1 cut(s) 524
Esp3I CGTCTC 1 cut(s) 997
FaeI CATG 8 cut(s) 662, 673, 715, 934, 961, 965, 1184, 1217
FaqI GGGAC 3 cut(s) 106, 155, 782
FatI CATG 8 cut(s) 658, 669, 711, 930, 957, 961, 1180, 1213
FauNDI CATATG 1 cut(s) 691
FblI GTMKAC 1 cut(s) 562
Fnu4HI GCNGC 1 cut(s) 1092
FokI GGATG 2 cut(s) 377, 747
FriOI GRGCYC 1 cut(s) 625
Fsp4HI GCNGC 1 cut(s) 1092
FspBI CTAG 2 cut(s) 186, 423
GluI GCNGC 1 cut(s) 1092
GsaI CCCAGC 2 cut(s) 132, 1091
GsuI CTGGAG 1 cut(s) 636
HaeIII GGCC 7 cut(s) 66, 634, 716, 905, 944, 1086, 1114
HapII CCGG 3 cut(s) 20, 190, 1110
HgaI GACGC 1 cut(s) 1193
Hin1I GRCGYC 1 cut(s) 1006
Hin1II CATG 8 cut(s) 662, 673, 715, 934, 961, 965, 1184, 1217
HincII GTYRAC 1 cut(s) 1010
HindII GTYRAC 1 cut(s) 1010
HindIII AAGCTT 1 cut(s) 603
HinfI GANTC 4 cut(s) 284, 476, 740, 1130
HpaII CCGG 3 cut(s) 20, 190, 1110
HphI GGTGA 3 cut(s) 407, 426, 1205
Hpy166II GTNNAC 6 cut(s) 60, 373, 398, 458, 563, 1010
Hpy188I TCNGA 6 cut(s) 136, 475, 708, 745, 760, 1209
Hpy188III TCNNGA 6 cut(s) 155, 172, 863, 899, 1173, 1181
Hpy8I GTNNAC 6 cut(s) 60, 373, 398, 458, 563, 1010
HpyAV CCTTC 3 cut(s) 244, 386, 1179
HpyCH4III ACNGT 4 cut(s) 462, 560, 796, 972
HpyCH4IV ACGT 2 cut(s) 565, 1006
HpyCH4V TGCA 7 cut(s) 270, 373, 577, 766, 961, 965, 1040
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 276, 298
HpyF3I CTNAG 2 cut(s) 198, 228
HpySE526I ACGT 2 cut(s) 565, 1006
Hsp92I GRCGYC 1 cut(s) 1006
Hsp92II CATG 8 cut(s) 662, 673, 715, 934, 961, 965, 1184, 1217
KpnI GGTACC 1 cut(s) 1147
Kzo9I GATC 3 cut(s) 425, 509, 755
LmnI GCTCC 3 cut(s) 620, 847, 1137
Lsp1109I GCAGC 1 cut(s) 1103
LweI GCATC 2 cut(s) 355, 985
MaeI CTAG 2 cut(s) 186, 423
MaeII ACGT 2 cut(s) 565, 1006
MaeIII GTNAC 6 cut(s) 418, 432, 462, 863, 1052, 1232
MalI GATC 3 cut(s) 427, 511, 757
MbiI CCGCTC 1 cut(s) 471
MboI GATC 3 cut(s) 425, 509, 755
MflI RGATCY 1 cut(s) 755
MhlI GDGCHC 3 cut(s) 294, 375, 625
MlsI TGGCCA 2 cut(s) 716, 944
MluNI TGGCCA 2 cut(s) 716, 944
Mox20I TGGCCA 2 cut(s) 716, 944
Mph1103I ATGCAT 1 cut(s) 963
MscI TGGCCA 2 cut(s) 716, 944
MseI TTAA 2 cut(s) 344, 350
Msp20I TGGCCA 2 cut(s) 716, 944
MspI CCGG 3 cut(s) 20, 190, 1110
MspR9I CCNGG 5 cut(s) 20, 72, 191, 589, 1111
Mva1269I GAATGC 1 cut(s) 169
MvaI CCWGG 2 cut(s) 72, 589
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 3 cut(s) 72, 276, 298
NciI CCSGG 3 cut(s) 20, 191, 1111
NdeI CATATG 1 cut(s) 691
NdeII GATC 3 cut(s) 425, 509, 755
NlaIII CATG 8 cut(s) 662, 673, 715, 934, 961, 965, 1184, 1217
NlaIV GGNNCC 3 cut(s) 6, 122, 1145
NmuCI GTSAC 3 cut(s) 418, 432, 863
NruI TCGCGA 1 cut(s) 156
NsiI ATGCAT 1 cut(s) 963
NspI RCATGY 3 cut(s) 662, 961, 965
PaeI GCATGC 2 cut(s) 961, 965
PaeR7I CTCGAG 1 cut(s) 624
PagI TCATGA 1 cut(s) 1180
PctI GAATGC 1 cut(s) 169
PfeI GAWTC 4 cut(s) 284, 476, 740, 1130
PkrI GCNGC 1 cut(s) 1093
PpuMI RGGWCCY 1 cut(s) 120
Psp124BI GAGCTC 1 cut(s) 625
Psp5II RGGWCCY 1 cut(s) 120
Psp6I CCWGG 2 cut(s) 70, 587
PspEI GGTNACC 1 cut(s) 432
PspFI CCCAGC 2 cut(s) 128, 1087
PspGI CCWGG 2 cut(s) 70, 587
PspN4I GGNNCC 3 cut(s) 6, 122, 1145
PspPI GGNCC 5 cut(s) 65, 120, 497, 1085, 1112
PspPPI RGGWCCY 1 cut(s) 120
PspXI VCTCGAGB 1 cut(s) 624
PstI CTGCAG 1 cut(s) 1042
PsuI RGATCY 1 cut(s) 755
RruI TCGCGA 1 cut(s) 156
RsaI GTAC 4 cut(s) 50, 59, 830, 1145
RsaNI GTAC 4 cut(s) 49, 58, 829, 1144
SacI GAGCTC 1 cut(s) 625
SaqAI TTAA 2 cut(s) 344, 350
SatI GCNGC 1 cut(s) 1092
Sau3AI GATC 3 cut(s) 425, 509, 755
Sau96I GGNCC 5 cut(s) 65, 120, 497, 1085, 1112
ScrFI CCNGG 5 cut(s) 20, 72, 191, 589, 1111
SduI GDGCHC 3 cut(s) 294, 375, 625
SfaNI GCATC 2 cut(s) 355, 985
SfcI CTRYAG 2 cut(s) 450, 1038
Sfr274I CTCGAG 1 cut(s) 624
SinI GGWCC 2 cut(s) 120, 497
SlaI CTCGAG 1 cut(s) 624
SmlI CTYRAG 3 cut(s) 414, 624, 949
SmoI CTYRAG 3 cut(s) 414, 624, 949
SphI GCATGC 2 cut(s) 961, 965
SsiI CCGC 2 cut(s) 279, 469
SspI AATATT 1 cut(s) 348
SspMI CTAG 2 cut(s) 186, 423
SstI GAGCTC 1 cut(s) 625
StyD4I CCNGG 5 cut(s) 18, 70, 189, 587, 1109
StyI CCWWGG 1 cut(s) 524
TaaI ACNGT 4 cut(s) 462, 560, 796, 972
TaiI ACGT 2 cut(s) 568, 1009
TaqI TCGA 2 cut(s) 625, 1240
TatI WGTACW 3 cut(s) 48, 57, 828
TfiI GAWTC 4 cut(s) 284, 476, 740, 1130
Tru1I TTAA 2 cut(s) 344, 350
Tru9I TTAA 2 cut(s) 344, 350
TscAI CASTG 4 cut(s) 67, 334, 977, 1042
TseFI GTSAC 3 cut(s) 418, 432, 863
TseI GCWGC 1 cut(s) 1091
Tsp45I GTSAC 3 cut(s) 418, 432, 863
TspDTI ATGAA 4 cut(s) 307, 678, 894, 947
TspRI CASTG 4 cut(s) 67, 334, 977, 1042
VneI GTGCAC 1 cut(s) 371
VpaK11BI GGWCC 2 cut(s) 120, 497
XapI RAATTY 5 cut(s) 314, 582, 646, 858, 979
XceI RCATGY 3 cut(s) 662, 961, 965
XhoI CTCGAG 1 cut(s) 624
XmiI GTMKAC 1 cut(s) 562
XspI CTAG 2 cut(s) 186, 423
ZraI GACGTC 1 cut(s) 1007
Zsp2I ATGCAT 1 cut(s) 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.