MD17G1278200.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006672
Physical Location & Seq
Forward (+)
33816801 .. 33821358
4558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1278200.v1.1.491

Sequence Viewer

Length: 1422 bp
ATGTTTAGAATTCATAAGGTTGAAATAATGGAAACGAGACCAGACCCGGATATCGATGACGACTTTAGCGAGCTATACAAGGAGTACACTGGCCCTCCTGGGTCAAATGCCGCTAACACACAAGAAAGGGCAAACCCAAACAAAAGGTCTCTTGCCGGGTCTGATGAGGAAGAGGAATCTCGCGATCCCAATGCCGTCCCAACCGATTTTACTAGCCGAGAAGCTAAGGTGTGGGAGGCCAAGTCAAAAGCAACTGAGAGGAATTGGAAGAAGAGGAAGGAAGAGGAAATGATTTGCAAAATCTGTGGGGAACCTGGCCACTTTACTCAGGGTTGCCCGTCTACCCTTGGTGCAAGTCGCAAGTCTCAAGATTTCTTTGAAAGGGTACCTGCTAGAGAAAAGCATGTAAGGTCCCTTTTCACTGAGAGAGTGATAAACAGGATTGAAAACGATATTGGATGCAATATTAAGATGGATGAGAAATTTATCATTGTCAGTGGCAAGGATAGGTTAGTATTAAGAAAGGGTGTGGATGCTGTGCACAAAATTATTAGAGAGGAAGGCGAACAAACAGATGGTTCTGCTTCTCGAGTGACTAGATCAAGGTCACCTGAGCGAAGTCCTGTTGGTGCACGTTTACAACGTTCTGAATCCCAGAGGTCTCATTCTGGTCCCCGAAACGGGTCGCATTTTCAACAAAGGTTTCACAGGCAAGAGAAATTTGTTGAAGACCGAATACGTCAGGATATGCAGAAAATTTCCAGGGGTTCTCCGCAAGCTTATGGTAATGACGGAGCTAGAGGTCGACCAAGTCATTCAAACTCTCCAGCTCGTGCCCCTTATATGAGAAACTCATATAATTCATATGATGGTCATAATCGGAGCATGGCTGCTTATAGAAATGATGGATGGAATTCTGATAAAAAAGGATCTGATATGCAATCCAGTCGTCAGTTTGATTACTCTGCCACCCCTCAGACATTAGAAGAATTAGAATTGGCGTACAAAAGAGAGGCAACGGAACTTGGAAGAATTCGTGACAAGGAAGAAGATGAAGAAAATTACGGACATCGTGAGGCGATTAGGGATATAAGAGAGAACCACGCGAAGAAATTGGCTACCCTGAGGGGCATGCATGCAAAGCAGTGGGAGGACTTTCTTCAACTTGATGCCCAGAAACGTCAACAACAGGCTAATCAGCAGATGTCGACTTCAGGTTTTGGTGGTTATAAATCACACAATTATTCTGAATACGACAGCTCCTCAGCCAATCCTCAGTATTCCATGGCTAATTTAGCCATGGATTCAAGGAGCAGGTACCCCAACACTATGGAAAACTATCCTTCAAGGCCTCACGACAATTTTGGTGAGTTTCAGCGTCAGAGGCACGAGGATTATGGGAGAGGTTACAATCGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

474

Amino Acids

54.73

Weight (kDa)

8.76

Isoelectric Point (pI)

61.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1230
Acc36I ACCTGC 2 cut(s) 397, 1305
Acc65I GGTACC 2 cut(s) 385, 1317
AccB1I GGYRCC 2 cut(s) 385, 1317
AccI GTMKAC 3 cut(s) 341, 805, 1208
AccII CGCG 2 cut(s) 183, 1106
AciI CCGC 2 cut(s) 111, 773
AclI AACGTT 1 cut(s) 643
AclWI GGATC 2 cut(s) 179, 937
AcoI YGGCCR 1 cut(s) 316
AcsI RAATTY 6 cut(s) 9, 482, 719, 756, 913, 1032
AcuI CTGAAG 1 cut(s) 1197
AfaI GTAC 4 cut(s) 86, 387, 1004, 1319
AfiI CCNNNNNNNGG 2 cut(s) 680, 681
AgsI TTSAA 9 cut(s) 23, 380, 446, 695, 728, 819, 1163, 1308, 1347
AjnI CCWGG 3 cut(s) 97, 313, 761
AjuI GAANNNNNNNTTGG 2 cut(s) 438, 470
AluBI AGCT 6 cut(s) 73, 224, 779, 797, 830, 1260
AluI AGCT 6 cut(s) 73, 224, 779, 797, 830, 1260
Alw21I GWGCWC 2 cut(s) 543, 634
Alw26I GTCTC 4 cut(s) 31, 153, 369, 666
Alw44I GTGCAC 2 cut(s) 539, 630
AlwI GGATC 2 cut(s) 179, 937
Ama87I CYCGRG 1 cut(s) 588
AoxI GGCC 4 cut(s) 91, 237, 316, 1349
ApaLI GTGCAC 2 cut(s) 539, 630
ApeKI GCWGC 1 cut(s) 890
ApoI RAATTY 6 cut(s) 9, 482, 719, 756, 913, 1032
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
Asp718I GGTACC 2 cut(s) 385, 1317
AspS9I GGNCC 3 cut(s) 92, 411, 671
AsuC2I CCSGG 2 cut(s) 47, 157
AsuHPI GGTGA 2 cut(s) 600, 1379
AvaI CYCGRG 1 cut(s) 588
AvaII GGWCC 2 cut(s) 411, 671
AxyI CCTNAGG 1 cut(s) 1124
BaeGI GKGCMC 3 cut(s) 543, 634, 838
BalI TGGCCA 1 cut(s) 318
BanI GGYRCC 2 cut(s) 385, 1317
BarI GAAGNNNNNNTAC 2 cut(s) 720, 752
BauI CACGAG 2 cut(s) 831, 1388
BbsI GAAGAC 1 cut(s) 735
Bbv12I GWGCWC 2 cut(s) 543, 634
BbvCI CCTCAGC 1 cut(s) 1264
BbvI GCAGC 1 cut(s) 877
BccI CCATC 5 cut(s) 466, 569, 863, 899, 903
BceAI ACGGC 1 cut(s) 179
BcgI CGANNNNNNTGC 4 cut(s) 173, 207, 929, 963
BciT130I CCWGG 3 cut(s) 99, 315, 763
BcnI CCSGG 2 cut(s) 47, 157
BcoDI GTCTC 4 cut(s) 31, 153, 369, 666
BfaI CTAG 4 cut(s) 213, 393, 597, 798
BfuAI ACCTGC 2 cut(s) 397, 1305
BisI GCNGC 2 cut(s) 111, 891
BlsI GCNGC 2 cut(s) 112, 892
Bme1390I CCNGG 5 cut(s) 47, 99, 157, 315, 763
Bme18I GGWCC 2 cut(s) 411, 671
BmeT110I CYCGRG 1 cut(s) 588
BmgT120I GGNCC 3 cut(s) 92, 411, 671
BmiI GGNNCC 5 cut(s) 312, 387, 413, 673, 1319
BmrFI CCNGG 5 cut(s) 47, 99, 157, 315, 763
BmsI GCATC 3 cut(s) 449, 523, 1159
BpiI GAAGAC 1 cut(s) 735
BpmI CTGGAG 1 cut(s) 810
Bpu10I CCTNAGC 3 cut(s) 225, 612, 1264
BpuEI CTTGAG 1 cut(s) 351
BpuMI CCSGG 2 cut(s) 47, 157
Bsa29I ATCGAT 2 cut(s) 54, 1414
BsaI GGTCTC 3 cut(s) 31, 153, 666
BsaJI CCNNGG 5 cut(s) 98, 346, 762, 1284, 1299
BsaXI ACNNNNNCTCC 6 cut(s) 79, 109, 227, 257, 1244, 1274
Bsc4I CCNNNNNNNGG 2 cut(s) 680, 681
Bse1I ACTGG 2 cut(s) 94, 945
Bse21I CCTNAGG 1 cut(s) 1124
BseBI CCWGG 3 cut(s) 99, 315, 763
BseCI ATCGAT 2 cut(s) 54, 1414
BseDI CCNNGG 5 cut(s) 98, 346, 762, 1284, 1299
BseGI GGATG 4 cut(s) 464, 481, 538, 914
BseLI CCNNNNNNNGG 2 cut(s) 680, 681
BseMII CTCAG 8 cut(s) 246, 341, 414, 603, 989, 1115, 1278, 1289
BseNI ACTGG 2 cut(s) 94, 945
BseRI GAGGAG 1 cut(s) 1252
BseSI GKGCMC 3 cut(s) 543, 634, 838
BseXI GCAGC 1 cut(s) 877
Bsh1236I CGCG 2 cut(s) 183, 1106
BshFI GGCC 4 cut(s) 93, 239, 318, 1351
BshNI GGYRCC 2 cut(s) 385, 1317
BshVI ATCGAT 2 cut(s) 54, 1414
BsiHKAI GWGCWC 2 cut(s) 543, 634
BsiHKCI CYCGRG 1 cut(s) 588
BsiSI CCGG 2 cut(s) 47, 156
BslFI GGGAC 3 cut(s) 182, 397, 657
BslI CCNNNNNNNGG 2 cut(s) 680, 681
BsmAI GTCTC 4 cut(s) 31, 153, 369, 666
BsmFI GGGAC 3 cut(s) 182, 397, 657
BsnI GGCC 4 cut(s) 93, 239, 318, 1351
Bso31I GGTCTC 3 cut(s) 31, 153, 666
BsoBI CYCGRG 1 cut(s) 588
Bsp1286I GDGCHC 3 cut(s) 543, 634, 838
Bsp143I GATC 3 cut(s) 184, 599, 929
Bsp19I CCATGG 2 cut(s) 1284, 1299
Bsp68I TCGCGA 1 cut(s) 183
BspACI CCGC 2 cut(s) 111, 773
BspANI GGCC 4 cut(s) 93, 239, 318, 1351
BspCNI CTCAG 8 cut(s) 247, 340, 415, 604, 988, 1116, 1277, 1288
BspDI ATCGAT 2 cut(s) 54, 1414
BspFNI CGCG 2 cut(s) 183, 1106
BspLI GGNNCC 5 cut(s) 312, 387, 413, 673, 1319
BspMI ACCTGC 2 cut(s) 397, 1305
BspPI GGATC 2 cut(s) 179, 937
BspT107I GGYRCC 2 cut(s) 385, 1317
BspTNI GGTCTC 3 cut(s) 31, 153, 666
BsrI ACTGG 2 cut(s) 94, 945
BssECI CCNNGG 5 cut(s) 98, 346, 762, 1284, 1299
BssMI GATC 3 cut(s) 184, 599, 929
BssSI CACGAG 2 cut(s) 831, 1388
BssT1I CCWWGG 3 cut(s) 346, 1284, 1299
Bst2BI CACGAG 2 cut(s) 831, 1388
Bst2UI CCWGG 3 cut(s) 99, 315, 763
Bst6I CTCTTC 3 cut(s) 165, 266, 276
BstC8I GCNNGC 4 cut(s) 71, 777, 1133, 1137
BstDEI CTNAG 9 cut(s) 225, 255, 327, 423, 612, 975, 1124, 1264, 1275
BstDSI CCRYGG 2 cut(s) 1284, 1299
BstEII GGTNACC 1 cut(s) 606
BstF5I GGATG 4 cut(s) 464, 481, 538, 914
BstFNI CGCG 2 cut(s) 183, 1106
BstKTI GATC 3 cut(s) 187, 602, 932
BstMAI GTCTC 4 cut(s) 31, 153, 369, 666
BstMBI GATC 3 cut(s) 184, 599, 929
BstMWI GCNNNNNNNGC 3 cut(s) 1141, 1295, 1384
BstNI CCWGG 3 cut(s) 99, 315, 763
BstNSI RCATGY 3 cut(s) 407, 1135, 1139
BstPI GGTNACC 1 cut(s) 606
BstSCI CCNGG 5 cut(s) 45, 97, 155, 313, 761
BstSLI GKGCMC 3 cut(s) 543, 634, 838
BstUI CGCG 2 cut(s) 183, 1106
BstV1I GCAGC 1 cut(s) 877
BstV2I GAAGAC 1 cut(s) 735
BstX2I RGATCY 1 cut(s) 929
BstXI CCANNNNNNTGG 1 cut(s) 1330
BstYI RGATCY 1 cut(s) 929
Bsu15I ATCGAT 2 cut(s) 54, 1414
Bsu36I CCTNAGG 1 cut(s) 1124
BsuRI GGCC 4 cut(s) 93, 239, 318, 1351
BsuTUI ATCGAT 2 cut(s) 54, 1414
BtgI CCRYGG 2 cut(s) 1284, 1299
BtsCI GGATG 4 cut(s) 464, 481, 538, 914
BtsI GCAGTG 1 cut(s) 1151
BtsIMutI CAGTG 4 cut(s) 87, 420, 502, 1151
BtuMI TCGCGA 1 cut(s) 183
BveI ACCTGC 2 cut(s) 397, 1305
Cac8I GCNNGC 4 cut(s) 71, 777, 1133, 1137
Cfr13I GGNCC 3 cut(s) 92, 411, 671
ClaI ATCGAT 2 cut(s) 54, 1414
CseI GACGC 1 cut(s) 1367
Csp6I GTAC 4 cut(s) 85, 386, 1003, 1318
CspCI CAANNNNNGTGG 2 cut(s) 286, 321
CviAII CATG 6 cut(s) 404, 886, 1132, 1136, 1285, 1300
CviQI GTAC 4 cut(s) 85, 386, 1003, 1318
DdeI CTNAG 9 cut(s) 225, 255, 327, 423, 612, 975, 1124, 1264, 1275
DpnI GATC 3 cut(s) 186, 601, 931
DpnII GATC 3 cut(s) 184, 599, 929
EaeI YGGCCR 1 cut(s) 316
Eam1104I CTCTTC 3 cut(s) 165, 266, 276
EarI CTCTTC 3 cut(s) 165, 266, 276
Eco130I CCWWGG 3 cut(s) 346, 1284, 1299
Eco147I AGGCCT 1 cut(s) 1351
Eco31I GGTCTC 3 cut(s) 31, 153, 666
Eco32I GATATC 1 cut(s) 52
Eco47I GGWCC 2 cut(s) 411, 671
Eco57I CTGAAG 1 cut(s) 1197
Eco81I CCTNAGG 1 cut(s) 1124
Eco88I CYCGRG 1 cut(s) 588
Eco91I GGTNACC 1 cut(s) 606
EcoO109I RGGNCCY 1 cut(s) 411
EcoO65I GGTNACC 1 cut(s) 606
EcoRI GAATTC 3 cut(s) 9, 913, 1032
EcoRII CCWGG 3 cut(s) 97, 313, 761
EcoRV GATATC 1 cut(s) 52
EcoT14I CCWWGG 3 cut(s) 346, 1284, 1299
EcoT22I ATGCAT 1 cut(s) 1137
ErhI CCWWGG 3 cut(s) 346, 1284, 1299
FaeI CATG 6 cut(s) 407, 889, 1135, 1139, 1288, 1303
FaqI GGGAC 3 cut(s) 182, 397, 657
FatI CATG 6 cut(s) 403, 885, 1131, 1135, 1284, 1299
FauNDI CATATG 1 cut(s) 865
FblI GTMKAC 3 cut(s) 341, 805, 1208
Fnu4HI GCNGC 2 cut(s) 111, 891
FokI GGATG 4 cut(s) 471, 488, 545, 921
Fsp4HI GCNGC 2 cut(s) 111, 891
FspBI CTAG 4 cut(s) 213, 393, 597, 798
GluI GCNGC 2 cut(s) 111, 891
GsuI CTGGAG 1 cut(s) 810
HaeIII GGCC 4 cut(s) 93, 239, 318, 1351
HapII CCGG 2 cut(s) 47, 156
HgaI GACGC 1 cut(s) 1367
Hin1II CATG 6 cut(s) 407, 889, 1135, 1139, 1288, 1303
HincII GTYRAC 3 cut(s) 806, 1184, 1209
HindII GTYRAC 3 cut(s) 806, 1184, 1209
HindIII AAGCTT 1 cut(s) 777
HinfI GANTC 3 cut(s) 176, 650, 1304
HpaII CCGG 2 cut(s) 47, 156
HphI GGTGA 2 cut(s) 600, 1379
Hpy166II GTNNAC 8 cut(s) 87, 342, 541, 632, 638, 806, 1184, 1209
Hpy188I TCNGA 8 cut(s) 163, 649, 882, 919, 934, 978, 1249, 1383
Hpy188III TCNNGA 7 cut(s) 182, 368, 588, 743, 1037, 1073, 1355
Hpy8I GTNNAC 8 cut(s) 87, 342, 541, 632, 638, 806, 1184, 1209
HpyAV CCTTC 3 cut(s) 271, 554, 1353
HpyCH4IV ACGT 4 cut(s) 634, 643, 739, 1180
HpyCH4V TGCA 9 cut(s) 297, 353, 462, 541, 632, 751, 940, 1135, 1139
HpyF10VI GCNNNNNNNGC 3 cut(s) 1141, 1295, 1384
HpyF3I CTNAG 9 cut(s) 225, 255, 327, 423, 612, 975, 1124, 1264, 1275
HpySE526I ACGT 4 cut(s) 634, 643, 739, 1180
Hsp92II CATG 6 cut(s) 407, 889, 1135, 1139, 1288, 1303
KpnI GGTACC 2 cut(s) 389, 1321
Kzo9I GATC 3 cut(s) 184, 599, 929
LmnI GCTCC 4 cut(s) 794, 882, 1265, 1311
Lsp1109I GCAGC 1 cut(s) 877
LweI GCATC 3 cut(s) 449, 523, 1159
MaeI CTAG 4 cut(s) 213, 393, 597, 798
MaeII ACGT 4 cut(s) 634, 643, 739, 1180
MaeIII GTNAC 4 cut(s) 592, 606, 1037, 1406
MalI GATC 3 cut(s) 186, 601, 931
MboI GATC 3 cut(s) 184, 599, 929
MflI RGATCY 1 cut(s) 929
MhlI GDGCHC 3 cut(s) 543, 634, 838
MlsI TGGCCA 1 cut(s) 318
MluNI TGGCCA 1 cut(s) 318
Mox20I TGGCCA 1 cut(s) 318
Mph1103I ATGCAT 1 cut(s) 1137
MscI TGGCCA 1 cut(s) 318
MseI TTAA 2 cut(s) 468, 518
Msp20I TGGCCA 1 cut(s) 318
MspI CCGG 2 cut(s) 47, 156
MspR9I CCNGG 5 cut(s) 47, 99, 157, 315, 763
MvaI CCWGG 3 cut(s) 99, 315, 763
MvnI CGCG 2 cut(s) 183, 1106
MwoI GCNNNNNNNGC 3 cut(s) 1141, 1295, 1384
NciI CCSGG 2 cut(s) 47, 157
NcoI CCATGG 2 cut(s) 1284, 1299
NdeI CATATG 1 cut(s) 865
NdeII GATC 3 cut(s) 184, 599, 929
NlaIII CATG 6 cut(s) 407, 889, 1135, 1139, 1288, 1303
NlaIV GGNNCC 5 cut(s) 312, 387, 413, 673, 1319
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 3 cut(s) 592, 606, 1037
NruI TCGCGA 1 cut(s) 183
NsiI ATGCAT 1 cut(s) 1137
NspI RCATGY 3 cut(s) 407, 1135, 1139
PaeI GCATGC 2 cut(s) 1135, 1139
PaeR7I CTCGAG 1 cut(s) 588
PceI AGGCCT 1 cut(s) 1351
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 640
PfeI GAWTC 3 cut(s) 176, 650, 1304
PflFI GACNNNGTC 1 cut(s) 810
PkrI GCNGC 2 cut(s) 112, 892
PpuMI RGGWCCY 1 cut(s) 411
PsiI TTATAA 1 cut(s) 1230
Psp1406I AACGTT 1 cut(s) 643
Psp5II RGGWCCY 1 cut(s) 411
Psp6I CCWGG 3 cut(s) 97, 313, 761
PspEI GGTNACC 1 cut(s) 606
PspGI CCWGG 3 cut(s) 97, 313, 761
PspN4I GGNNCC 5 cut(s) 312, 387, 413, 673, 1319
PspPI GGNCC 3 cut(s) 92, 411, 671
PspPPI RGGWCCY 1 cut(s) 411
PsuI RGATCY 1 cut(s) 929
PsyI GACNNNGTC 1 cut(s) 810
RruI TCGCGA 1 cut(s) 183
RsaI GTAC 4 cut(s) 86, 387, 1004, 1319
RsaNI GTAC 4 cut(s) 85, 386, 1003, 1318
SalI GTCGAC 2 cut(s) 804, 1207
SaqAI TTAA 2 cut(s) 468, 518
SatI GCNGC 2 cut(s) 111, 891
Sau3AI GATC 3 cut(s) 184, 599, 929
Sau96I GGNCC 3 cut(s) 92, 411, 671
ScrFI CCNGG 5 cut(s) 47, 99, 157, 315, 763
SduI GDGCHC 3 cut(s) 543, 634, 838
SfaNI GCATC 3 cut(s) 449, 523, 1159
Sfr274I CTCGAG 1 cut(s) 588
SinI GGWCC 2 cut(s) 411, 671
SlaI CTCGAG 1 cut(s) 588
SmlI CTYRAG 2 cut(s) 366, 588
SmoI CTYRAG 2 cut(s) 366, 588
SphI GCATGC 2 cut(s) 1135, 1139
SseBI AGGCCT 1 cut(s) 1351
SsiI CCGC 2 cut(s) 111, 773
SspI AATATT 1 cut(s) 466
SspMI CTAG 4 cut(s) 213, 393, 597, 798
StuI AGGCCT 1 cut(s) 1351
StyD4I CCNGG 5 cut(s) 45, 97, 155, 313, 761
StyI CCWWGG 3 cut(s) 346, 1284, 1299
TaiI ACGT 4 cut(s) 637, 646, 742, 1183
TaqI TCGA 5 cut(s) 54, 589, 805, 1208, 1414
TaqII GACCGA 1 cut(s) 747
TatI WGTACW 1 cut(s) 84
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 3 cut(s) 176, 650, 1304
Tru1I TTAA 2 cut(s) 468, 518
Tru9I TTAA 2 cut(s) 468, 518
TscAI CASTG 4 cut(s) 94, 427, 502, 1151
TseFI GTSAC 3 cut(s) 592, 606, 1037
TseI GCWGC 1 cut(s) 890
Tsp45I GTSAC 3 cut(s) 592, 606, 1037
TspDTI ATGAA 2 cut(s) 852, 1068
TspGWI ACGGA 3 cut(s) 807, 1034, 1080
TspRI CASTG 4 cut(s) 94, 427, 502, 1151
Tth111I GACNNNGTC 1 cut(s) 810
VneI GTGCAC 2 cut(s) 539, 630
VpaK11BI GGWCC 2 cut(s) 411, 671
XapI RAATTY 6 cut(s) 9, 482, 719, 756, 913, 1032
XceI RCATGY 3 cut(s) 407, 1135, 1139
XhoI CTCGAG 1 cut(s) 588
XmiI GTMKAC 3 cut(s) 341, 805, 1208
XspI CTAG 4 cut(s) 213, 393, 597, 798
Zsp2I ATGCAT 1 cut(s) 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.