MD10G1005000.v1.1

Protein of unknown function (DUF1442)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013601
Physical Location & Seq
Forward (+)
808167 .. 809399
1233 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1005000.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGAATGGTCTCCTCAAGCTGCCTTGAAAGCTTACTTAGATACTCTTCAGCTGTGTAAGTTTCACAATGATCAAGACTTGGTGCAAGGAAACACATGTAGTAGTGGTATTATAGAACCAAAATGCATGGAATTTATATCAGCTTTAGCAGCTGGAAAGCGAGCAAAGTTAATGTTGCAAATCACATCTGAAGGTGTAACACCGCTGACGGTTTCACTTGCGGTGGCTGCAAAGCAAAGTGGAGGCCGACTGATCGTGTGCATTAATAACGACGACGACGATCATACGGAGAAGTTTGGCAAAACCCTACTTTTGGAAAACGGTCTCGACGACGTCGTTGAATATGTTTACGATACTGATCCTTGTAGAATGGTGAAGCATTTCAAGAATGTTGAATTTGCGGCTGTGGACTGCAAGTTTAAAGATTACTTGAAACTTTTGAAGGTTATGAATCTCAACCCAAATGGGAGTATAGTGGTGATAAACAATATTGGTGTGGCTGTTAATGGAGGTCCAATTCTCGATCGAGTTTTCAAGGAAAAGAAAGCATTTAAATATGTGACCTTACCTTTAGGAGAAGGAATGGAGTTCATAAGATTCAGATCCAAGGGCAAGTGCAAATCCCACAGATATAAGAGATTTCATGTAACGTTTGAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1442

Protein Analysis

221

Amino Acids

24.66

Weight (kDa)

8.59

Isoelectric Point (pI)

22.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1442 PF07279 3 - 212 6e-23 Protein of unknown function (DUF1442)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013601)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00920
malus_domestica MD10G1005000.v1.1
prunus_persica Prupe.8G007800_v2.0.a1
pyrus_communis pycom10g00400
rosa_chinensis RchiOBHm_Chr6g0251271
rosa_laevigata RLG00000015468
rosa_multiflora Rmu_co8193732.1_g000001 Rmu_sc0001637.1_g000006 Rmu_sc0015396.1_g000007
rosa_roxburghii Rroxscaffold_7G00216860
rosa_rugosa Rorug05G0501400
rosa_samantha Rh6AG010800 Rh6BG001200 Rh6CG007800 Rh6DG008200
rosa_wichuraiana Rw6G001040 Rw6G001150 Rw6G001220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 336
AciI CCGC 3 cut(s) 203, 221, 401
AclI AACGTT 1 cut(s) 650
AclWI GGATC 2 cut(s) 353, 597
AcsI RAATTY 2 cut(s) 131, 395
AcuI CTGAAG 2 cut(s) 32, 210
AcyI GRCGYC 1 cut(s) 333
AfiI CCNNNNNNNGG 1 cut(s) 313
AflIII ACRYGT 1 cut(s) 95
AgsI TTSAA 8 cut(s) 28, 341, 385, 395, 433, 442, 535, 656
AluBI AGCT 5 cut(s) 20, 32, 52, 143, 152
AluI AGCT 5 cut(s) 20, 32, 52, 143, 152
Alw26I GTCTC 2 cut(s) 15, 329
AlwI GGATC 2 cut(s) 353, 597
AoxI GGCC 1 cut(s) 244
ApeKI GCWGC 3 cut(s) 20, 149, 227
ApoI RAATTY 2 cut(s) 131, 395
AseI ATTAAT 1 cut(s) 264
Asp700I GAANNNNTTC 1 cut(s) 380
AspS9I GGNCC 1 cut(s) 512
AsuHPI GGTGA 2 cut(s) 385, 490
AvaII GGWCC 1 cut(s) 512
BbvI GCAGC 3 cut(s) 7, 161, 214
BclI TGATCA 1 cut(s) 70
BcoDI GTCTC 2 cut(s) 15, 329
BisI GCNGC 4 cut(s) 21, 150, 228, 402
BlsI GCNGC 4 cut(s) 22, 151, 229, 403
Bme18I GGWCC 1 cut(s) 512
BmgT120I GGNCC 1 cut(s) 512
BsaBI GATNNNNATC 2 cut(s) 357, 601
BsaHI GRCGYC 1 cut(s) 333
BsaI GGTCTC 2 cut(s) 15, 329
BsaJI CCNNGG 1 cut(s) 606
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse8I GATNNNNATC 2 cut(s) 357, 601
BseDI CCNNGG 1 cut(s) 606
BseJI GATNNNNATC 2 cut(s) 357, 601
BseLI CCNNNNNNNGG 1 cut(s) 313
BseXI GCAGC 3 cut(s) 7, 161, 214
Bsh1285I CGRYCG 1 cut(s) 526
BshFI GGCC 1 cut(s) 246
BsiEI CGRYCG 1 cut(s) 526
BslI CCNNNNNNNGG 1 cut(s) 313
BsmAI GTCTC 2 cut(s) 15, 329
BsnI GGCC 1 cut(s) 246
Bso31I GGTCTC 2 cut(s) 15, 329
Bsp143I GATC 6 cut(s) 70, 252, 280, 358, 523, 602
BspACI CCGC 3 cut(s) 203, 221, 401
BspANI GGCC 1 cut(s) 246
BspPI GGATC 2 cut(s) 353, 597
BspTNI GGTCTC 2 cut(s) 15, 329
BssECI CCNNGG 1 cut(s) 606
BssMI GATC 6 cut(s) 70, 252, 280, 358, 523, 602
BssNI GRCGYC 1 cut(s) 333
BssT1I CCWWGG 1 cut(s) 606
Bst4CI ACNGT 2 cut(s) 211, 323
Bst6I CTCTTC 1 cut(s) 51
BstACI GRCGYC 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 1 cut(s) 37
BstKTI GATC 6 cut(s) 73, 255, 283, 361, 526, 605
BstMAI GTCTC 2 cut(s) 15, 329
BstMBI GATC 6 cut(s) 70, 252, 280, 358, 523, 602
BstMCI CGRYCG 1 cut(s) 526
BstMWI GCNNNNNNNGC 3 cut(s) 29, 149, 227
BstNSI RCATGY 1 cut(s) 99
BstV1I GCAGC 3 cut(s) 7, 161, 214
BstX2I RGATCY 1 cut(s) 602
BstYI RGATCY 1 cut(s) 602
BsuRI GGCC 1 cut(s) 246
Cac8I GCNNGC 1 cut(s) 162
Cfr13I GGNCC 1 cut(s) 512
CviAII CATG 3 cut(s) 96, 127, 644
CviJI RGCY 9 cut(s) 20, 32, 52, 143, 152, 227, 246, 404, 500
CviKI_1 RGCY 9 cut(s) 20, 32, 52, 143, 152, 227, 246, 404, 500
DdeI CTNAG 1 cut(s) 37
DpnI GATC 6 cut(s) 72, 254, 282, 360, 525, 604
DpnII GATC 6 cut(s) 70, 252, 280, 358, 523, 602
DraI TTTAAA 2 cut(s) 421, 553
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 606
Eco31I GGTCTC 2 cut(s) 15, 329
Eco47I GGWCC 1 cut(s) 512
Eco57I CTGAAG 2 cut(s) 32, 210
EcoT14I CCWWGG 1 cut(s) 606
EcoT22I ATGCAT 1 cut(s) 128
ErhI CCWWGG 1 cut(s) 606
FaeI CATG 3 cut(s) 99, 130, 647
FatI CATG 3 cut(s) 95, 126, 643
FbaI TGATCA 1 cut(s) 70
Fnu4HI GCNGC 4 cut(s) 21, 150, 228, 402
Fsp4HI GCNGC 4 cut(s) 21, 150, 228, 402
GluI GCNGC 4 cut(s) 21, 150, 228, 402
HaeIII GGCC 1 cut(s) 246
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 3 cut(s) 99, 130, 647
HindIII AAGCTT 1 cut(s) 30
HinfI GANTC 2 cut(s) 451, 597
HphI GGTGA 2 cut(s) 385, 490
Hpy166II GTNNAC 2 cut(s) 349, 409
Hpy188I TCNGA 2 cut(s) 190, 602
Hpy188III TCNNGA 4 cut(s) 74, 326, 385, 521
Hpy8I GTNNAC 2 cut(s) 349, 409
Hpy99I CGWCG 6 cut(s) 275, 278, 281, 332, 335, 338
HpyAV CCTTC 3 cut(s) 185, 436, 572
HpyCH4III ACNGT 2 cut(s) 211, 323
HpyCH4IV ACGT 2 cut(s) 333, 650
HpyCH4V TGCA 7 cut(s) 85, 126, 178, 230, 261, 414, 618
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 149, 227
HpyF3I CTNAG 1 cut(s) 37
HpySE526I ACGT 2 cut(s) 333, 650
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 3 cut(s) 99, 130, 647
Ksp22I TGATCA 1 cut(s) 70
Kzo9I GATC 6 cut(s) 70, 252, 280, 358, 523, 602
LpnPI CCDG 1 cut(s) 138
Lsp1109I GCAGC 3 cut(s) 7, 161, 214
MaeII ACGT 2 cut(s) 333, 650
MaeIII GTNAC 3 cut(s) 196, 559, 646
MalI GATC 6 cut(s) 72, 254, 282, 360, 525, 604
MboI GATC 6 cut(s) 70, 252, 280, 358, 523, 602
MboII GAAGA 1 cut(s) 38
MflI RGATCY 1 cut(s) 602
MluCI AATT 4 cut(s) 131, 395, 516, 658
MnlI CCTC 3 cut(s) 24, 236, 503
Mph1103I ATGCAT 1 cut(s) 128
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 6 cut(s) 170, 264, 420, 504, 552, 661
MspA1I CMGCKG 3 cut(s) 52, 152, 205
MwoI GCNNNNNNNGC 3 cut(s) 29, 149, 227
NdeII GATC 6 cut(s) 70, 252, 280, 358, 523, 602
NlaIII CATG 3 cut(s) 99, 130, 647
NmuCI GTSAC 1 cut(s) 559
NsiI ATGCAT 1 cut(s) 128
NspI RCATGY 1 cut(s) 99
PciI ACATGT 1 cut(s) 95
PcsI WCGNNNNNNNCGW 3 cut(s) 276, 327, 333
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 2 cut(s) 451, 597
PflFI GACNNNGTC 1 cut(s) 332
PkrI GCNGC 4 cut(s) 22, 151, 229, 403
Ple19I CGATCG 1 cut(s) 526
PscI ACATGT 1 cut(s) 95
PshBI ATTAAT 1 cut(s) 264
Psp1406I AACGTT 1 cut(s) 650
PspPI GGNCC 1 cut(s) 512
PsuI RGATCY 1 cut(s) 602
PsyI GACNNNGTC 1 cut(s) 332
PvuI CGATCG 1 cut(s) 526
PvuII CAGCTG 2 cut(s) 52, 152
SaqAI TTAA 6 cut(s) 170, 264, 420, 504, 552, 661
SatI GCNGC 4 cut(s) 21, 150, 228, 402
Sau3AI GATC 6 cut(s) 70, 252, 280, 358, 523, 602
Sau96I GGNCC 1 cut(s) 512
SinI GGWCC 1 cut(s) 512
SmiI ATTTAAAT 1 cut(s) 553
SmlI CTYRAG 1 cut(s) 15
SmoI CTYRAG 1 cut(s) 15
Sse9I AATT 4 cut(s) 131, 395, 516, 658
SsiI CCGC 3 cut(s) 203, 221, 401
SspI AATATT 1 cut(s) 490
StyI CCWWGG 1 cut(s) 606
SwaI ATTTAAAT 1 cut(s) 553
TaaI ACNGT 2 cut(s) 211, 323
TaiI ACGT 2 cut(s) 336, 653
TaqI TCGA 3 cut(s) 327, 522, 526
TasI AATT 4 cut(s) 131, 395, 516, 658
TauI GCSGC 1 cut(s) 404
TfiI GAWTC 2 cut(s) 451, 597
Tru1I TTAA 6 cut(s) 170, 264, 420, 504, 552, 661
Tru9I TTAA 6 cut(s) 170, 264, 420, 504, 552, 661
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 3 cut(s) 20, 149, 227
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 3 cut(s) 464, 580, 632
TspGWI ACGGA 1 cut(s) 302
Tth111I GACNNNGTC 1 cut(s) 332
VpaK11BI GGWCC 1 cut(s) 512
VspI ATTAAT 1 cut(s) 264
XapI RAATTY 2 cut(s) 131, 395
XceI RCATGY 1 cut(s) 99
XmnI GAANNNNTTC 1 cut(s) 380
ZraI GACGTC 1 cut(s) 334
Zsp2I ATGCAT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.