Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0008971

Physical Location & Seq

Genomic Coordinates Reverse (-)

940729 .. 946419

Gene Structure 5691 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1006100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2403 bp

ATGGCTGTGGGGAAGTATTCCCGAGTTGATGGAAGGAAGTCGTCGAATTGTTGTTCAACGACTAGTCTGGTTGTTTTTGTAGCGTTTTGTTTAATTGGGGTCTGGATGTTGATGTCAACGGTTGTTCCGATTCAGAATCAAGGGTCATCATCTGAGGAGACCATGGATGAGGTAAACCGGCAAGTTATAACTGAAAAAAATTCAAAGCAATTCGAAGACAGTTCGGGGGAACTATCAGGAACTTTAGCAAAAGAGGATGAAAATGGCAGTGGTGAAAGCCATTTGGATATTGAGAATGACCAAAATGTTGAGGATGGGTCTGTGAATACAACGGAAGAAAAACAGGAAGAAGTGGTTTCAAAGGAGGGATCTGAGGAGAAGACTGAGCCAGAAAGTGACAATGGTGGTGAGGGAAATCAGCAAGAGAAGCTTGTGGAAGAGAGATCTGATGAGAAACCTTTGATCGAAGAAGGAAAAAAGACACAGGAAATAACTGATCAAGAAGCTAGTTCAGGAGATGGGGAGTCCAATTCCGAAGCTGGAAGAACGGAGGTGGAAGAGAGTTCAGGTAGAAAGACATCTGGAGAGGAGACTTCTGATGAAGTAAAGCAGGAAGAGGAAGCTAATAGTCAGACTGAGACGGAAAAGGTTGAGAAAGATCAGGAAGAAAGTGATACCAGTGGTAACACAGAAAGCCAGGAAACTACAGCTGAAAATGAGCAAGGGACAACTGAAGTTGTGGAGTCGCAGAATGAGAAGGAAACCCAAGAATCTTCGTCAGCTGAACATAAGAGTGGGCATCATGAATGGAAGTGGAAAGTCTGTGATGTCACGGCTGGGCCAGACTACATCCCTTGTCTTGACAATTGGGGATCTATCAAGAAGCTTCCAGGTAGAGGCCACTATGAACACCGAGAGAGGCACTGCCCTGATGAAGCCCCCACCTGTCTTGTTCCCCTACCAGAAGGATATAAACGACCCATTCAGTGGCCCACAAGCAGGGAAAAGATATGGTACCACAATGTCCCTCACACGAAGCTCGCAGAAGTTAAAGGGCACCAAAATTGGGTTAAAGTTACCGGTGAATACCTCACATTTCCCGGTGGCGGAACTCAGTTTATAAATGGCGCTCTTCATTACATTGATTTTATTCAAAAATCTCTTCCTGATATCGCATGGGGTAAAAGAAGCCGTGTGATATTGGATGTTGGGTGTGGTGTAGCTAGCTTTGGAGGATTTCTATTTGAAAGAGATGTTCTTGCTATGTCATTTGCTCCTAAGGATGAGCATGAAGCCCAAGTACAATTTGCACTAGAACGGGGAATCCCTGCCATATCAGCTGTTATGGGTACCAAAAGACTACCCTACCCAAGTTGTGTGTTTGATCTTATCCACTGTGCACGGTGTAGAGTCCCTTGGCATATTGAAGGGGGTAAACTGCTCCTGGAGCTTAATCGTGTCTTGCGACCTGGTGGTTACTTTGTCTGGTCTGCTACTCCAGTTTACCAAAAGCTTCCTGAAGATGTTGGCATTTGGAAAGCTATGTCGAAATTAACAAAGTCAATGTGCTGGGATCTAGTGGTTATTAAAAAGGACAAACTCAATGGAGTGGCTGCTGCCATATATAGAAAACCCTCTACCAATGAGTGCTACAATAAAAGATCACGCAATGACCCTCCTCTCTGCAGTGAATCTGATGATCCAAATGCTGGCTGGAATGTAACATTGGAGGCATGCATGCACAAAGTCCCGGAGGGTGTATTAGACCGTGGATCTCAGTGGCCTGCACAATGGCCATCAAGGTTGCAGAAACCACCATACTGGTTAAAGTCTCAGCTTGATGTTGATGGGAAATCTGCCCAAGAAGATTTCATTTCTGACTACAAGCATTGGAAAAAAGTTGTTTCCGAGACCTATTTGAATGGGATGGGAATCAATTGGTCTACTGTGAGGAATGTCATGGACATGAGAGCTGTATACGGAGGGTTTGCTGCAGCTCTAAAAGACTTAAAAGTTTGGGTTATGAATGTAGTCCCAGTTGACTCTCGTGACACACTTCCAATAATCTATGAGCGCGGTTTAATTGGGATATATCATGATTGGTGTGAATCGTTCAGTACCTACCCCAGATCTTATGATCTTTTACACGCAGATCATCTATTCTCTGTCCTTAAGAAAAGCTTAGTGGCAGTGATTGCAGAAGTTGATAGGATACTGAGGCCAGAAGGGAAACTAATTGTATGGGACAACGCTGAAACCTTAAATGAGATTGAGAGTATGGCTAAATCTCTGCAATGGGACATCCGATTTACATACTCGAAAGGCAATGAGGGTTTGCTGTACATTCAGAAGACGTTTTGGCGTCCTGCAGAGAAGGAAACAATTCTGTCAGCCATTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005911 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0009506 GO:0012505 GO:0016740 GO:0016741 GO:0030054 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0055044 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

801

Amino Acids

89.95

Weight (kDa)

5.01

Isoelectric Point (pI)

46.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	282 - 780	1.5e-224	Putative S-adenosyl-L-methionine-dependent methyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008971)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29470 AT1G29470 AT2G34300 AT2G34300 AT2G34300
fragaria_vesca	FvH4_2g00990 FvH4_2g00990
malus_domestica	MD05G1008600.v1.1 MD10G1006100.v1.1
prunus_persica	Prupe.8G010300_v2.0.a1
pyrus_communis	pycom10g00450
rosa_chinensis	RchiOBHm_Chr6g0251511
rosa_laevigata	RLG00000015461
rosa_multiflora	Rmu_sc0001382.1_g000020 Rmu_sc0040206.1_g000001
rosa_roxburghii	Rroxscaffold_7G00216610
rosa_rugosa	Rorug05G0502800 Rorug05G0502900
rosa_samantha	Rh6AG011600 Rh6BG010400 Rh6CG009000
rosa_wichuraiana	Rw6G001340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	188, 1121
Acc65I	GGTACC	2 cut(s)	1014, 1349
AccB1I	GGYRCC	3 cut(s)	1014, 1056, 1349
AccB7I	CCANNNNNTGG	2 cut(s)	987, 1709
AccI	GTMKAC	2 cut(s)	1943, 1977
AccII	CGCG	1 cut(s)	2076
AciI	CCGC	2 cut(s)	1107, 2076
AclWI	GGATC	5 cut(s)	376, 880, 1581, 1694, 1780
AcoI	YGGCCR	1 cut(s)	1793
AcsI	RAATTY	1 cut(s)	199
AcuI	CTGAAG	2 cut(s)	753, 1539
AcyI	GRCGYC	1 cut(s)	2362
AfaI	GTAC	5 cut(s)	1016, 1302, 1351, 2119, 2342
AfiI	CCNNNNNNNGG	6 cut(s)	896, 987, 999, 1066, 1106, 1709
AflII	CTTAAG	1 cut(s)	2171
AgeI	ACCGGT	1 cut(s)	1079
AgsI	TTSAA	7 cut(s)	57, 204, 360, 1154, 1247, 1427, 1921
AhlI	ACTAGT	1 cut(s)	62
AjnI	CCWGG	4 cut(s)	696, 889, 1443, 1468
AjuI	GAANNNNNNNTTGG	2 cut(s)	1709, 1741
AloI	GAACNNNNNNTCC	2 cut(s)	1308, 1340
Alw21I	GWGCWC	1 cut(s)	1402
Alw26I	GTCTC	5 cut(s)	152, 584, 632, 1836, 1904
Alw44I	GTGCAC	1 cut(s)	1398
AlwI	GGATC	5 cut(s)	376, 880, 1581, 1694, 1780
Ama87I	CYCGRG	1 cut(s)	21
AoxI	GGCC	6 cut(s)	839, 898, 989, 1781, 1793, 2219
ApaLI	GTGCAC	1 cut(s)	1398
ApeKI	GCWGC	4 cut(s)	1613, 1616, 1991, 1994
ApoI	RAATTY	1 cut(s)	199
AsiGI	ACCGGT	1 cut(s)	1079
Asp700I	GAANNNNTTC	1 cut(s)	2382
Asp718I	GGTACC	2 cut(s)	1014, 1349
AspLEI	GCGC	2 cut(s)	1130, 2076
AspS9I	GGNCC	2 cut(s)	839, 990
AsuC2I	CCSGG	2 cut(s)	1101, 1751
AsuHPI	GGTGA	3 cut(s)	284, 419, 1094
AsuII	TTCGAA	1 cut(s)	213
AsuNHI	GCTAGC	1 cut(s)	1223
AvaI	CYCGRG	1 cut(s)	21
AxyI	CCTNAGG	1 cut(s)	1278
BaeGI	GKGCMC	2 cut(s)	1059, 1402
BalI	TGGCCA	1 cut(s)	1795
BanI	GGYRCC	3 cut(s)	1014, 1056, 1349
BarI	GAAGNNNNNNTAC	2 cut(s)	1284, 1316
BauI	CACGAG	1 cut(s)	2046
BbsI	GAAGAC	3 cut(s)	222, 386, 2357
Bbv12I	GWGCWC	1 cut(s)	1402
BbvI	GCAGC	4 cut(s)	1600, 1603, 1978, 2006
BccI	CCATC	6 cut(s)	23, 308, 512, 1804, 1841, 1921
BceAI	ACGGC	2 cut(s)	849, 1176
BciT130I	CCWGG	4 cut(s)	698, 891, 1445, 1470
BciVI	GTATCC	1 cut(s)	2205
BclI	TGATCA	1 cut(s)	496
BcnI	CCSGG	2 cut(s)	1101, 1751
BcoDI	GTCTC	5 cut(s)	152, 584, 632, 1836, 1904
BcuI	ACTAGT	1 cut(s)	62
BfaI	CTAG	5 cut(s)	63, 507, 1224, 1313, 1577
BfmI	CTRYAG	4 cut(s)	705, 1684, 1992, 2367
BfoI	RGCGCY	1 cut(s)	1131
BfrI	CTTAAG	1 cut(s)	2171
BfuI	GTATCC	1 cut(s)	2205
BglII	AGATCT	2 cut(s)	443, 2129
BisI	GCNGC	4 cut(s)	1614, 1617, 1992, 1995
BlsI	GCNGC	4 cut(s)	1615, 1618, 1993, 1996
Bme1390I	CCNGG	6 cut(s)	698, 891, 1101, 1445, 1470, 1751
BmeT110I	CYCGRG	1 cut(s)	21
BmgT120I	GGNCC	2 cut(s)	839, 990
BmiI	GGNNCC	3 cut(s)	1016, 1058, 1351
BmrFI	CCNGG	6 cut(s)	698, 891, 1101, 1445, 1470, 1751
BmrI	ACTGGG	1 cut(s)	2030
BmsI	GCATC	1 cut(s)	808
BmtI	GCTAGC	1 cut(s)	1227
BmuI	ACTGGG	1 cut(s)	2030
BpiI	GAAGAC	3 cut(s)	222, 386, 2357
BpmI	CTGGAG	3 cut(s)	603, 1466, 1482
Bpu14I	TTCGAA	1 cut(s)	213
BpuMI	CCSGG	2 cut(s)	1101, 1751
BsaBI	GATNNNNATC	1 cut(s)	1931
BsaHI	GRCGYC	1 cut(s)	2362
BsaI	GGTCTC	2 cut(s)	152, 1904
BsaJI	CCNNGG	3 cut(s)	162, 1415, 1768
BsaWI	WCCGGW	1 cut(s)	1079
Bsc4I	CCNNNNNNNGG	6 cut(s)	896, 987, 999, 1066, 1106, 1709
Bse118I	RCCGGY	2 cut(s)	177, 1079
Bse1I	ACTGG	4 cut(s)	678, 1499, 1826, 2036
Bse21I	CCTNAGG	1 cut(s)	1278
Bse3DI	GCAATG	4 cut(s)	1675, 2300, 2332, 2394
Bse8I	GATNNNNATC	1 cut(s)	1931
BseBI	CCWGG	4 cut(s)	698, 891, 1445, 1470
BseDI	CCNNGG	3 cut(s)	162, 1415, 1768
BseGI	GGATG	9 cut(s)	111, 172, 262, 319, 849, 1210, 1288, 1932, 2301
BseJI	GATNNNNATC	1 cut(s)	1931
BseLI	CCNNNNNNNGG	6 cut(s)	896, 987, 999, 1066, 1106, 1709
BseMI	GCAATG	4 cut(s)	1675, 2300, 2332, 2394
BseMII	CTCAG	8 cut(s)	144, 363, 375, 627, 1127, 1790, 1847, 2207
BseNI	ACTGG	4 cut(s)	678, 1499, 1826, 2036
BseRI	GAGGAG	4 cut(s)	170, 389, 602, 1668
BseSI	GKGCMC	2 cut(s)	1059, 1402
BseXI	GCAGC	4 cut(s)	1600, 1603, 1978, 2006
BseYI	CCCAGC	2 cut(s)	836, 1569
BsgI	GTGCAG	1 cut(s)	1770
Bsh1236I	CGCG	1 cut(s)	2076
BshFI	GGCC	6 cut(s)	841, 900, 991, 1783, 1795, 2221
BshNI	GGYRCC	3 cut(s)	1014, 1056, 1349
BshTI	ACCGGT	1 cut(s)	1079
BsiHKAI	GWGCWC	1 cut(s)	1402
BsiHKCI	CYCGRG	1 cut(s)	21
BsiSI	CCGG	4 cut(s)	178, 1080, 1101, 1751
BslFI	GGGAC	7 cut(s)	739, 1010, 1397, 1733, 2018, 2258, 2312
BslI	CCNNNNNNNGG	6 cut(s)	896, 987, 999, 1066, 1106, 1709
BsmAI	GTCTC	5 cut(s)	152, 584, 632, 1836, 1904
BsmBI	CGTCTC	1 cut(s)	632
BsmFI	GGGAC	7 cut(s)	739, 1010, 1397, 1733, 2018, 2258, 2312
BsnI	GGCC	6 cut(s)	841, 900, 991, 1783, 1795, 2221
Bso31I	GGTCTC	2 cut(s)	152, 1904
BsoBI	CYCGRG	1 cut(s)	21
Bsp119I	TTCGAA	1 cut(s)	213
Bsp1286I	GDGCHC	2 cut(s)	1059, 1402
Bsp1407I	TGTACA	1 cut(s)	2340
Bsp19I	CCATGG	1 cut(s)	162
BspACI	CCGC	2 cut(s)	1107, 2076
BspANI	GGCC	6 cut(s)	841, 900, 991, 1783, 1795, 2221
BspCNI	CTCAG	8 cut(s)	145, 364, 376, 628, 1126, 1789, 1846, 2208
BspFNI	CGCG	1 cut(s)	2076
BspHI	TCATGA	2 cut(s)	802, 2095
BspLI	GGNNCC	3 cut(s)	1016, 1058, 1351
BspMAI	CTGCAG	3 cut(s)	1688, 1996, 2371
BspOI	GCTAGC	1 cut(s)	1227
BspPI	GGATC	5 cut(s)	376, 880, 1581, 1694, 1780
BspQI	GCTCTTC	1 cut(s)	1137
BspT104I	TTCGAA	1 cut(s)	213
BspT107I	GGYRCC	3 cut(s)	1014, 1056, 1349
BspTI	CTTAAG	1 cut(s)	2171
BspTNI	GGTCTC	2 cut(s)	152, 1904
BsrDI	GCAATG	4 cut(s)	1675, 2300, 2332, 2394
BsrFI	RCCGGY	2 cut(s)	177, 1079
BsrGI	TGTACA	1 cut(s)	2340
BsrI	ACTGG	4 cut(s)	678, 1499, 1826, 2036
BssAI	RCCGGY	2 cut(s)	177, 1079
BssECI	CCNNGG	3 cut(s)	162, 1415, 1768
BssNAI	GTATAC	1 cut(s)	1978
BssNI	GRCGYC	1 cut(s)	2362
BssSI	CACGAG	1 cut(s)	2046
BssT1I	CCWWGG	2 cut(s)	162, 1415
Bst1107I	GTATAC	1 cut(s)	1978
Bst2BI	CACGAG	1 cut(s)	2046
Bst2UI	CCWGG	4 cut(s)	698, 891, 1445, 1470
Bst4CI	ACNGT	6 cut(s)	121, 221, 1397, 1404, 1769, 1948
Bst6I	CTCTTC	5 cut(s)	432, 552, 609, 1137, 1167
BstACI	GRCGYC	1 cut(s)	2362
BstAFI	CTTAAG	1 cut(s)	2171
BstAPI	GCANNNNNTGC	1 cut(s)	2195
BstAUI	TGTACA	1 cut(s)	2340
BstBI	TTCGAA	1 cut(s)	213
BstC8I	GCNNGC	6 cut(s)	1041, 1225, 1711, 1735, 1739, 1785
BstDSI	CCRYGG	2 cut(s)	162, 1768
BstF5I	GGATG	9 cut(s)	111, 172, 262, 319, 849, 1210, 1288, 1932, 2301
BstFNI	CGCG	1 cut(s)	2076
BstH2I	RGCGCY	1 cut(s)	1131
BstHHI	GCGC	2 cut(s)	1130, 2076
BstMAI	GTCTC	5 cut(s)	152, 584, 632, 1836, 1904
BstMWI	GCNNNNNNNGC	4 cut(s)	427, 1337, 1447, 2195
BstNI	CCWGG	4 cut(s)	698, 891, 1445, 1470
BstNSI	RCATGY	2 cut(s)	1737, 1741
BstSCI	CCNGG	6 cut(s)	696, 889, 1099, 1443, 1468, 1749
BstSFI	CTRYAG	4 cut(s)	705, 1684, 1992, 2367
BstSLI	GKGCMC	2 cut(s)	1059, 1402
BstUI	CGCG	1 cut(s)	2076
BstV1I	GCAGC	4 cut(s)	1600, 1603, 1978, 2006
BstV2I	GAAGAC	3 cut(s)	222, 386, 2357
BstX2I	RGATCY	6 cut(s)	368, 443, 872, 1573, 1772, 2129
BstXI	CCANNNNNNTGG	1 cut(s)	1821
BstYI	RGATCY	6 cut(s)	368, 443, 872, 1573, 1772, 2129
BstZ17I	GTATAC	1 cut(s)	1978
Bsu36I	CCTNAGG	1 cut(s)	1278
BsuI	GTATCC	1 cut(s)	2205
BsuRI	GGCC	6 cut(s)	841, 900, 991, 1783, 1795, 2221
BtgI	CCRYGG	2 cut(s)	162, 1768
BtsCI	GGATG	9 cut(s)	111, 172, 262, 319, 849, 1210, 1288, 1932, 2301
BtsI	GCAGTG	4 cut(s)	274, 922, 1693, 2196
BtsIMutI	CAGTG	8 cut(s)	274, 685, 922, 992, 1393, 1693, 1784, 2196
Cac8I	GCNNGC	6 cut(s)	1041, 1225, 1711, 1735, 1739, 1785
CciI	TCATGA	2 cut(s)	802, 2095
CfoI	GCGC	2 cut(s)	1130, 2076
Cfr10I	RCCGGY	2 cut(s)	177, 1079
Cfr13I	GGNCC	2 cut(s)	839, 990
CseI	GACGC	1 cut(s)	2351
CsiI	ACCWGGT	1 cut(s)	1468
Csp6I	GTAC	5 cut(s)	1015, 1301, 1350, 2118, 2341
CspAI	ACCGGT	1 cut(s)	1079
CspCI	CAANNNNNGTGG	2 cut(s)	931, 966
CviAII	CATG	9 cut(s)	163, 803, 1176, 1289, 1734, 1738, 1960, 1966, 2096
CviQI	GTAC	5 cut(s)	1015, 1301, 1350, 2118, 2341
EaeI	YGGCCR	1 cut(s)	1793
Eam1104I	CTCTTC	5 cut(s)	432, 552, 609, 1137, 1167
EarI	CTCTTC	5 cut(s)	432, 552, 609, 1137, 1167
EciI	GGCGGA	1 cut(s)	1122
Eco130I	CCWWGG	2 cut(s)	162, 1415
Eco31I	GGTCTC	2 cut(s)	152, 1904
Eco32I	GATATC	1 cut(s)	1171
Eco57I	CTGAAG	2 cut(s)	753, 1539
Eco81I	CCTNAGG	1 cut(s)	1278
Eco88I	CYCGRG	1 cut(s)	21
EcoRII	CCWGG	4 cut(s)	696, 889, 1443, 1468
EcoRV	GATATC	1 cut(s)	1171
EcoT14I	CCWWGG	2 cut(s)	162, 1415
EcoT22I	ATGCAT	1 cut(s)	1739
ErhI	CCWWGG	2 cut(s)	162, 1415
Esp3I	CGTCTC	1 cut(s)	632
FaeI	CATG	9 cut(s)	166, 806, 1179, 1292, 1737, 1741, 1963, 1969, 2099
FalI	AAGNNNNNCTT	4 cut(s)	414, 446, 2165, 2197
FaqI	GGGAC	7 cut(s)	739, 1010, 1397, 1733, 2018, 2258, 2312
FatI	CATG	9 cut(s)	162, 802, 1175, 1288, 1733, 1737, 1959, 1965, 2095
FbaI	TGATCA	1 cut(s)	496
FblI	GTMKAC	2 cut(s)	1943, 1977
Fnu4HI	GCNGC	4 cut(s)	1614, 1617, 1992, 1995
FokI	GGATG	9 cut(s)	118, 179, 269, 326, 836, 1217, 1295, 1939, 2288
Fsp4HI	GCNGC	4 cut(s)	1614, 1617, 1992, 1995
FspBI	CTAG	5 cut(s)	63, 507, 1224, 1313, 1577
GlaI	GCGC	2 cut(s)	1129, 2075
GluI	GCNGC	4 cut(s)	1614, 1617, 1992, 1995
GsaI	CCCAGC	2 cut(s)	840, 1573
GsuI	CTGGAG	3 cut(s)	603, 1466, 1482
HaeII	RGCGCY	1 cut(s)	1131
HaeIII	GGCC	6 cut(s)	841, 900, 991, 1783, 1795, 2221
HapII	CCGG	4 cut(s)	178, 1080, 1101, 1751
HgaI	GACGC	1 cut(s)	2351
HhaI	GCGC	2 cut(s)	1130, 2076
Hin1I	GRCGYC	1 cut(s)	2362
Hin1II	CATG	9 cut(s)	166, 806, 1179, 1292, 1737, 1741, 1963, 1969, 2099
Hin6I	GCGC	2 cut(s)	1128, 2074
HinP1I	GCGC	2 cut(s)	1128, 2074
HincII	GTYRAC	2 cut(s)	117, 2041
HindII	GTYRAC	2 cut(s)	117, 2041
HindIII	AAGCTT	4 cut(s)	428, 884, 1511, 2179
HpaII	CCGG	4 cut(s)	178, 1080, 1101, 1751
HphI	GGTGA	3 cut(s)	284, 419, 1094
Hpy166II	GTNNAC	8 cut(s)	117, 175, 1400, 1436, 1504, 1944, 1978, 2041
Hpy8I	GTNNAC	8 cut(s)	117, 175, 1400, 1436, 1504, 1944, 1978, 2041
Hpy99I	CGWCG	1 cut(s)	46
HpyAV	CCTTC	7 cut(s)	27, 464, 751, 959, 1421, 2219, 2368
HpyCH4III	ACNGT	6 cut(s)	121, 221, 1397, 1404, 1769, 1948
HpyCH4IV	ACGT	1 cut(s)	2354
HpyF10VI	GCNNNNNNNGC	4 cut(s)	427, 1337, 1447, 2195
HpySE526I	ACGT	1 cut(s)	2354
Hsp92I	GRCGYC	1 cut(s)	2362
Hsp92II	CATG	9 cut(s)	166, 806, 1179, 1292, 1737, 1741, 1963, 1969, 2099
HspAI	GCGC	2 cut(s)	1128, 2074
KpnI	GGTACC	2 cut(s)	1018, 1353
Ksp22I	TGATCA	1 cut(s)	496
LguI	GCTCTTC	1 cut(s)	1137
LmnI	GCTCC	3 cut(s)	1279, 1446, 1447
Lsp1109I	GCAGC	4 cut(s)	1600, 1603, 1978, 2006
LweI	GCATC	1 cut(s)	808
MabI	ACCWGGT	1 cut(s)	1468
MaeI	CTAG	5 cut(s)	63, 507, 1224, 1313, 1577
MaeII	ACGT	1 cut(s)	2354
MaeIII	GTNAC	7 cut(s)	395, 683, 829, 1075, 1475, 1720, 2048
MfeI	CAATTG	2 cut(s)	865, 1936
MflI	RGATCY	6 cut(s)	368, 443, 872, 1573, 1772, 2129
MhlI	GDGCHC	2 cut(s)	1059, 1402
MlsI	TGGCCA	1 cut(s)	1795
MluNI	TGGCCA	1 cut(s)	1795
MlyI	GAGTC	4 cut(s)	533, 752, 1419, 2036
Mox20I	TGGCCA	1 cut(s)	1795
Mph1103I	ATGCAT	1 cut(s)	1739
MroXI	GAANNNNTTC	1 cut(s)	2382
MscI	TGGCCA	1 cut(s)	1795
MslI	CAYNNNNRTG	2 cut(s)	792, 1964
Msp20I	TGGCCA	1 cut(s)	1795
MspA1I	CMGCKG	3 cut(s)	710, 782, 1340
MspCI	CTTAAG	1 cut(s)	2171
MspI	CCGG	4 cut(s)	178, 1080, 1101, 1751
MspR9I	CCNGG	6 cut(s)	698, 891, 1101, 1445, 1470, 1751
MunI	CAATTG	2 cut(s)	865, 1936
MvaI	CCWGG	4 cut(s)	698, 891, 1445, 1470
MvnI	CGCG	1 cut(s)	2076
MwoI	GCNNNNNNNGC	4 cut(s)	427, 1337, 1447, 2195
NciI	CCSGG	2 cut(s)	1101, 1751
NcoI	CCATGG	1 cut(s)	162
NheI	GCTAGC	1 cut(s)	1223
NlaIII	CATG	9 cut(s)	166, 806, 1179, 1292, 1737, 1741, 1963, 1969, 2099
NlaIV	GGNNCC	3 cut(s)	1016, 1058, 1351
NmuCI	GTSAC	3 cut(s)	395, 829, 2048
NsiI	ATGCAT	1 cut(s)	1739
NspI	RCATGY	2 cut(s)	1737, 1741
NspV	TTCGAA	1 cut(s)	213
PaeI	GCATGC	2 cut(s)	1737, 1741
PagI	TCATGA	2 cut(s)	802, 2095
PciSI	GCTCTTC	1 cut(s)	1137
PcsI	WCGNNNNNNNCGW	2 cut(s)	125, 1462
PdmI	GAANNNNTTC	1 cut(s)	2382
PfeI	GAWTC	7 cut(s)	130, 136, 770, 1323, 1691, 1932, 2108
PflMI	CCANNNNNTGG	2 cut(s)	987, 1709
PfoI	TCCNGGA	2 cut(s)	1443, 1749
PinAI	ACCGGT	1 cut(s)	1079
PkrI	GCNGC	4 cut(s)	1615, 1618, 1993, 1996
PleI	GAGTC	4 cut(s)	532, 751, 1418, 2036
PpsI	GAGTC	4 cut(s)	532, 751, 1418, 2036
PsiI	TTATAA	2 cut(s)	188, 1121
Psp6I	CCWGG	4 cut(s)	696, 889, 1443, 1468
PspFI	CCCAGC	2 cut(s)	836, 1569
PspGI	CCWGG	4 cut(s)	696, 889, 1443, 1468
PspN4I	GGNNCC	3 cut(s)	1016, 1058, 1351
PspPI	GGNCC	2 cut(s)	839, 990
PstI	CTGCAG	3 cut(s)	1688, 1996, 2371
PsuI	RGATCY	6 cut(s)	368, 443, 872, 1573, 1772, 2129
PvuII	CAGCTG	3 cut(s)	710, 782, 1340
RsaI	GTAC	5 cut(s)	1016, 1302, 1351, 2119, 2342
RsaNI	GTAC	5 cut(s)	1015, 1301, 1350, 2118, 2341
RseI	CAYNNNNRTG	2 cut(s)	792, 1964
SapI	GCTCTTC	1 cut(s)	1137
SatI	GCNGC	4 cut(s)	1614, 1617, 1992, 1995
Sau96I	GGNCC	2 cut(s)	839, 990
SchI	GAGTC	4 cut(s)	533, 752, 1419, 2036
ScrFI	CCNGG	6 cut(s)	698, 891, 1101, 1445, 1470, 1751
SduI	GDGCHC	2 cut(s)	1059, 1402
SexAI	ACCWGGT	1 cut(s)	1468
SfaNI	GCATC	1 cut(s)	808
SfcI	CTRYAG	4 cut(s)	705, 1684, 1992, 2367
SfuI	TTCGAA	1 cut(s)	213
SmiMI	CAYNNNNRTG	2 cut(s)	792, 1964
SmlI	CTYRAG	1 cut(s)	2171
SmoI	CTYRAG	1 cut(s)	2171
SpeI	ACTAGT	1 cut(s)	62
SphI	GCATGC	2 cut(s)	1737, 1741
SsiI	CCGC	2 cut(s)	1107, 2076
SspMI	CTAG	5 cut(s)	63, 507, 1224, 1313, 1577
StyD4I	CCNGG	6 cut(s)	696, 889, 1099, 1443, 1468, 1749
StyI	CCWWGG	2 cut(s)	162, 1415
TaaI	ACNGT	6 cut(s)	121, 221, 1397, 1404, 1769, 1948
TaiI	ACGT	1 cut(s)	2357
TaqI	TCGA	5 cut(s)	44, 213, 465, 1547, 2318
TatI	WGTACW	2 cut(s)	1300, 2340
TfiI	GAWTC	7 cut(s)	130, 136, 770, 1323, 1691, 1932, 2108
TscAI	CASTG	8 cut(s)	274, 685, 929, 992, 1400, 1693, 1784, 2196
TseFI	GTSAC	3 cut(s)	395, 829, 2048
TseI	GCWGC	4 cut(s)	1613, 1616, 1991, 1994
Tsp45I	GTSAC	3 cut(s)	395, 829, 2048
TspDTI	ATGAA	9 cut(s)	273, 615, 819, 921, 948, 1124, 1305, 1861, 2039
TspGWI	ACGGA	4 cut(s)	347, 563, 656, 1995
TspRI	CASTG	8 cut(s)	274, 685, 929, 992, 1400, 1693, 1784, 2196
Van91I	CCANNNNNTGG	2 cut(s)	987, 1709
Vha464I	CTTAAG	1 cut(s)	2171
VneI	GTGCAC	1 cut(s)	1398
XapI	RAATTY	1 cut(s)	199
XceI	RCATGY	2 cut(s)	1737, 1741
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1710
XmiI	GTMKAC	2 cut(s)	1943, 1977
XmnI	GAANNNNTTC	1 cut(s)	2382
XspI	CTAG	5 cut(s)	63, 507, 1224, 1313, 1577
Zsp2I	ATGCAT	1 cut(s)	1739

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.