Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0015473

Physical Location & Seq

Genomic Coordinates Forward (+)

3680522 .. 3684058

Gene Structure 3537 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1028500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 630 bp

ATGGCGATTTCCGTGACGGCGTCCGCGATTCCTTGTCTCAGCTCTGACCGTACGGCTCGCATAGTTGCGGCGGCGGCATCATCCTCTTCTTCTTGTTCAAAGCTGTCCTCCTCGACTTCTCTGCGATTTCCATCACACCTTCGACACGTTCGGATCGGAACACGGGACTCACGGCCTCGGATTCTTCCTCCGGTTGAAGCAAAGAAGCAAACATTTTCCTCCTTCGAAGATTTGCTGGCTACCTCCGAAAAACCTGTGTTGGTTGACTTCTATGCAACCTGGTGCGGTCCTTGTCAGTTTATGGCACCTATCCTCAATGAAGTCAGCATTACGCTGAATGACAAGATCCAGGTTGTGAAAATTGACACGGAGAAGTATCCTAGCATTGCTGATAAATATAGCATACAAGCATTGCCTACTTTCATCATATTTAAGGATGGAGAGCCATTTGACCGCTTTGAGGGTGCTCTGACTGCTGATCAGCTTGTCGAACGAATAGAAACTACTTTGAAACTTCTCAGGTTGAACCGAATCGATTCAGCCGCATCTGAGGGGATATGGATGAAATTTATTAATTGCCTTCGGGACATTCATTTCATATGTGATGAAATTTATTCTAGGCTTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004791 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0006979 GO:0008047 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009636 GO:0009987 GO:0015035 GO:0015036 GO:0016209 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0016671 GO:0019725 GO:0030234 GO:0033554 GO:0034599 GO:0042221 GO:0042592 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044093 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045454 GO:0047134 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051716 GO:0055114 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0097237 GO:0098754 GO:0098772 GO:0098869 GO:1990748

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Thioredoxin

Protein Analysis

210

Amino Acids

23.32

Weight (kDa)

7.59

Isoelectric Point (pI)

45.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Thioredoxin	PF00085	70 - 167	1.6e-29	Thioredoxin
Thioredoxin_2	PF13098	81 - 164	1.1e-09	Thioredoxin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015473)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76760
fragaria_vesca	FvH4_2g13460
malus_domestica	MD05G1027800.v1.1 MD10G1028500.v1.1
prunus_persica	Prupe.8G033500_v2.0.a1
pyrus_communis	pycom10g01850
rosa_chinensis	RchiOBHm_Chr6g0275081
rosa_laevigata	RLG00000013499
rosa_multiflora	Rmu_sc0000447.1_g000014
rosa_roxburghii	Rroxscaffold_7G00193680
rosa_rugosa	Rorug06G0088000
rosa_samantha	Rh6AG202300 Rh6BG205700 Rh6CG207600 Rh6DG197500
rosa_wichuraiana	Rw6G017570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	304
AccII	CGCG	1 cut(s)	26
AciI	CCGC	7 cut(s)	24, 68, 71, 74, 285, 454, 543
AclWI	GGATC	2 cut(s)	161, 340
AcsI	RAATTY	2 cut(s)	566, 609
AcyI	GRCGYC	1 cut(s)	20
AfaI	GTAC	1 cut(s)	52
AfiI	CCNNNNNNNGG	1 cut(s)	460
AflIII	ACRYGT	1 cut(s)	145
AgsI	TTSAA	4 cut(s)	99, 197, 511, 526
AjnI	CCWGG	2 cut(s)	278, 348
AluBI	AGCT	3 cut(s)	42, 103, 484
AluI	AGCT	3 cut(s)	42, 103, 484
Alw21I	GWGCWC	1 cut(s)	469
Alw26I	GTCTC	1 cut(s)	41
AlwI	GGATC	2 cut(s)	161, 340
AoxI	GGCC	1 cut(s)	173
ApoI	RAATTY	2 cut(s)	566, 609
AseI	ATTAAT	1 cut(s)	573
Asp700I	GAANNNNTTC	1 cut(s)	535
AspS9I	GGNCC	1 cut(s)	287
AsuII	TTCGAA	1 cut(s)	225
AvaII	GGWCC	1 cut(s)	287
BanI	GGYRCC	1 cut(s)	304
Bbv12I	GWGCWC	1 cut(s)	469
BccI	CCATC	2 cut(s)	139, 431
BceAI	ACGGC	3 cut(s)	33, 69, 188
BcgI	CGANNNNNNTGC	2 cut(s)	103, 137
BciT130I	CCWGG	2 cut(s)	280, 350
BciVI	GTATCC	1 cut(s)	387
BclI	TGATCA	1 cut(s)	478
BcoDI	GTCTC	1 cut(s)	41
BfaI	CTAG	2 cut(s)	381, 618
BfuI	GTATCC	1 cut(s)	387
BisI	GCNGC	4 cut(s)	69, 72, 75, 543
BlsI	GCNGC	4 cut(s)	70, 73, 76, 544
Bme1390I	CCNGG	2 cut(s)	280, 350
Bme18I	GGWCC	1 cut(s)	287
BmgT120I	GGNCC	1 cut(s)	287
BmiI	GGNNCC	1 cut(s)	306
BmrFI	CCNGG	2 cut(s)	280, 350
BmsI	GCATC	2 cut(s)	86, 554
Bpu14I	TTCGAA	1 cut(s)	225
Bsa29I	ATCGAT	1 cut(s)	534
BsaBI	GATNNNNATC	1 cut(s)	130
BsaHI	GRCGYC	1 cut(s)	20
BsaJI	CCNNGG	1 cut(s)	176
BsaWI	WCCGGW	1 cut(s)	190
Bsc4I	CCNNNNNNNGG	1 cut(s)	460
Bse3DI	GCAATG	2 cut(s)	384, 410
Bse8I	GATNNNNATC	1 cut(s)	130
BseBI	CCWGG	2 cut(s)	280, 350
BseCI	ATCGAT	1 cut(s)	534
BseDI	CCNNGG	1 cut(s)	176
BseGI	GGATG	3 cut(s)	80, 442, 567
BseJI	GATNNNNATC	1 cut(s)	130
BseLI	CCNNNNNNNGG	1 cut(s)	460
BseMI	GCAATG	2 cut(s)	384, 410
BseMII	CTCAG	3 cut(s)	52, 532, 540
BseRI	GAGGAG	1 cut(s)	100
Bsh1236I	CGCG	1 cut(s)	26
BshFI	GGCC	1 cut(s)	175
BshNI	GGYRCC	1 cut(s)	304
BshVI	ATCGAT	1 cut(s)	534
BsiHKAI	GWGCWC	1 cut(s)	469
BsiSI	CCGG	1 cut(s)	191
BsiWI	CGTACG	1 cut(s)	50
BslFI	GGGAC	2 cut(s)	179, 599
BslI	CCNNNNNNNGG	1 cut(s)	460
BsmAI	GTCTC	1 cut(s)	41
BsmFI	GGGAC	2 cut(s)	179, 599
BsnI	GGCC	1 cut(s)	175
Bsp119I	TTCGAA	1 cut(s)	225
Bsp1286I	GDGCHC	1 cut(s)	469
Bsp143I	GATC	3 cut(s)	153, 345, 478
BspACI	CCGC	7 cut(s)	24, 68, 71, 74, 285, 454, 543
BspANI	GGCC	1 cut(s)	175
BspCNI	CTCAG	3 cut(s)	51, 531, 541
BspDI	ATCGAT	1 cut(s)	534
BspFNI	CGCG	1 cut(s)	26
BspLI	GGNNCC	1 cut(s)	306
BspPI	GGATC	2 cut(s)	161, 340
BspT104I	TTCGAA	1 cut(s)	225
BspT107I	GGYRCC	1 cut(s)	304
BsrDI	GCAATG	2 cut(s)	384, 410
BssECI	CCNNGG	1 cut(s)	176
BssMI	GATC	3 cut(s)	153, 345, 478
BssNI	GRCGYC	1 cut(s)	20
Bst2UI	CCWGG	2 cut(s)	280, 350
Bst4CI	ACNGT	1 cut(s)	50
Bst6I	CTCTTC	1 cut(s)	91
BstACI	GRCGYC	1 cut(s)	20
BstBI	TTCGAA	1 cut(s)	225
BstC8I	GCNNGC	2 cut(s)	58, 237
BstDEI	CTNAG	3 cut(s)	38, 518, 549
BstF5I	GGATG	3 cut(s)	80, 442, 567
BstFNI	CGCG	1 cut(s)	26
BstKTI	GATC	3 cut(s)	156, 348, 481
BstMAI	GTCTC	1 cut(s)	41
BstMBI	GATC	3 cut(s)	153, 345, 478
BstMWI	GCNNNNNNNGC	2 cut(s)	74, 473
BstNI	CCWGG	2 cut(s)	280, 350
BstSCI	CCNGG	2 cut(s)	278, 348
BstUI	CGCG	1 cut(s)	26
BstX2I	RGATCY	1 cut(s)	345
BstYI	RGATCY	1 cut(s)	345
Bsu15I	ATCGAT	1 cut(s)	534
BsuI	GTATCC	1 cut(s)	387
BsuRI	GGCC	1 cut(s)	175
BsuTUI	ATCGAT	1 cut(s)	534
BtsCI	GGATG	3 cut(s)	80, 442, 567
Cac8I	GCNNGC	2 cut(s)	58, 237
Cfr13I	GGNCC	1 cut(s)	287
ClaI	ATCGAT	1 cut(s)	534
CseI	GACGC	1 cut(s)	9
CsiI	ACCWGGT	1 cut(s)	278
Csp6I	GTAC	1 cut(s)	51
CviJI	RGCY	9 cut(s)	42, 56, 103, 175, 239, 445, 484, 542, 622
CviKI_1	RGCY	9 cut(s)	42, 56, 103, 175, 239, 445, 484, 542, 622
CviQI	GTAC	1 cut(s)	51
DdeI	CTNAG	3 cut(s)	38, 518, 549
DpnI	GATC	3 cut(s)	155, 347, 480
DpnII	GATC	3 cut(s)	153, 345, 478
Eam1104I	CTCTTC	1 cut(s)	91
EarI	CTCTTC	1 cut(s)	91
Eco47I	GGWCC	1 cut(s)	287
EcoRII	CCWGG	2 cut(s)	278, 348
FaiI	YATR	9 cut(s)	62, 273, 302, 399, 404, 428, 559, 599, 601
FaqI	GGGAC	2 cut(s)	179, 599
FauNDI	CATATG	1 cut(s)	599
FbaI	TGATCA	1 cut(s)	478
Fnu4HI	GCNGC	4 cut(s)	69, 72, 75, 543
FokI	GGATG	3 cut(s)	67, 449, 574
Fsp4HI	GCNGC	4 cut(s)	69, 72, 75, 543
FspBI	CTAG	2 cut(s)	381, 618
GluI	GCNGC	4 cut(s)	69, 72, 75, 543
HaeIII	GGCC	1 cut(s)	175
HapII	CCGG	1 cut(s)	191
HgaI	GACGC	1 cut(s)	9
Hin1I	GRCGYC	1 cut(s)	20
HincII	GTYRAC	1 cut(s)	265
HindII	GTYRAC	1 cut(s)	265
HinfI	GANTC	5 cut(s)	28, 167, 181, 531, 536
HpaII	CCGG	1 cut(s)	191
Hpy166II	GTNNAC	1 cut(s)	265
Hpy188I	TCNGA	7 cut(s)	46, 153, 158, 180, 247, 471, 550
Hpy188III	TCNNGA	1 cut(s)	584
Hpy8I	GTNNAC	1 cut(s)	265
HpyAV	CCTTC	3 cut(s)	149, 232, 590
HpyCH4III	ACNGT	1 cut(s)	50
HpyCH4IV	ACGT	1 cut(s)	147
HpyCH4V	TGCA	1 cut(s)	275
HpyF10VI	GCNNNNNNNGC	2 cut(s)	74, 473
HpyF3I	CTNAG	3 cut(s)	38, 518, 549
HpySE526I	ACGT	1 cut(s)	147
Hsp92I	GRCGYC	1 cut(s)	20
Ksp22I	TGATCA	1 cut(s)	478
Kzo9I	GATC	3 cut(s)	153, 345, 478
LpnPI	CCDG	8 cut(s)	204, 221, 265, 267, 292, 335, 362, 505
LweI	GCATC	2 cut(s)	86, 554
MabI	ACCWGGT	1 cut(s)	278
MaeI	CTAG	2 cut(s)	381, 618
MaeII	ACGT	1 cut(s)	147
MaeIII	GTNAC	1 cut(s)	13
MalI	GATC	3 cut(s)	155, 347, 480
MboI	GATC	3 cut(s)	153, 345, 478
MboII	GAAGA	4 cut(s)	78, 81, 176, 239
MflI	RGATCY	1 cut(s)	345
MhlI	GDGCHC	1 cut(s)	469
MluCI	AATT	4 cut(s)	360, 566, 574, 609
MlyI	GAGTC	1 cut(s)	161
MroXI	GAANNNNTTC	1 cut(s)	535
MseI	TTAA	2 cut(s)	432, 573
MspI	CCGG	1 cut(s)	191
MspR9I	CCNGG	2 cut(s)	280, 350
MvaI	CCWGG	2 cut(s)	280, 350
MvnI	CGCG	1 cut(s)	26
MwoI	GCNNNNNNNGC	2 cut(s)	74, 473
NdeI	CATATG	1 cut(s)	599
NdeII	GATC	3 cut(s)	153, 345, 478
NlaIV	GGNNCC	1 cut(s)	306
NmuCI	GTSAC	1 cut(s)	13
NspV	TTCGAA	1 cut(s)	225
PcsI	WCGNNNNNNNCGW	1 cut(s)	23
PdmI	GAANNNNTTC	1 cut(s)	535
PfeI	GAWTC	4 cut(s)	28, 181, 531, 536
Pfl23II	CGTACG	1 cut(s)	50
PflFI	GACNNNGTC	1 cut(s)	19
PkrI	GCNGC	4 cut(s)	70, 73, 76, 544
PleI	GAGTC	1 cut(s)	161
PpsI	GAGTC	1 cut(s)	161
PshBI	ATTAAT	1 cut(s)	573
Psp6I	CCWGG	2 cut(s)	278, 348
PspGI	CCWGG	2 cut(s)	278, 348
PspLI	CGTACG	1 cut(s)	50
PspN4I	GGNNCC	1 cut(s)	306
PspPI	GGNCC	1 cut(s)	287
PsuI	RGATCY	1 cut(s)	345
PsyI	GACNNNGTC	1 cut(s)	19
RsaI	GTAC	1 cut(s)	52
RsaNI	GTAC	1 cut(s)	51
SaqAI	TTAA	2 cut(s)	432, 573
SatI	GCNGC	4 cut(s)	69, 72, 75, 543
Sau3AI	GATC	3 cut(s)	153, 345, 478
Sau96I	GGNCC	1 cut(s)	287
SchI	GAGTC	1 cut(s)	161
ScrFI	CCNGG	2 cut(s)	280, 350
SduI	GDGCHC	1 cut(s)	469
SexAI	ACCWGGT	1 cut(s)	278
SfaNI	GCATC	2 cut(s)	86, 554
SfuI	TTCGAA	1 cut(s)	225
SinI	GGWCC	1 cut(s)	287
Sse9I	AATT	4 cut(s)	360, 566, 574, 609
SsiI	CCGC	7 cut(s)	24, 68, 71, 74, 285, 454, 543
SspMI	CTAG	2 cut(s)	381, 618
StyD4I	CCNGG	2 cut(s)	278, 348
TaaI	ACNGT	1 cut(s)	50
TaiI	ACGT	1 cut(s)	150
TaqI	TCGA	5 cut(s)	113, 142, 225, 489, 534
TasI	AATT	4 cut(s)	360, 566, 574, 609
TauI	GCSGC	4 cut(s)	71, 74, 77, 545
TfiI	GAWTC	4 cut(s)	28, 181, 531, 536
Tru1I	TTAA	2 cut(s)	432, 573
Tru9I	TTAA	2 cut(s)	432, 573
TseFI	GTSAC	1 cut(s)	13
Tsp45I	GTSAC	1 cut(s)	13
TspDTI	ATGAA	6 cut(s)	333, 412, 578, 581, 586, 621
TspGWI	ACGGA	1 cut(s)	383
Tth111I	GACNNNGTC	1 cut(s)	19
VpaK11BI	GGWCC	1 cut(s)	287
VspI	ATTAAT	1 cut(s)	573
XapI	RAATTY	2 cut(s)	566, 609
XmnI	GAANNNNTTC	1 cut(s)	535
XspI	CTAG	2 cut(s)	381, 618

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.