MD10G1038300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014414
Physical Location & Seq
Reverse (-)
5006739 .. 5008553
1815 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1038300.v1.1.491

Sequence Viewer

Length: 573 bp
ATGGACGAGAAAGCTGACAACAAAACCGGGGAAATAGAGGAAACGGGTCCAAAATCCGACGGGTATTCTCCGAACCCGAATCCGAATCCCAAAACTGCGAGGACGAAGGTTCCAGAAGTGGAAGTCTGCCTGTATCGACAAGGGAAAGGCCCGATCGACACGTTCAAGTCGGGTCTGGGAGGTTGGGACCAGAACCAGTTGGAGGTCGGAGACATTCTCGACAAGTACGGATTCAAATCGCTTTACGCTTTCAATACCGAATCGGGTCGCGGGGTTCCGATCCGGTTTAACCCTAGAAACGGCAGGTCTATGCTGCCGTATAGAGATGGAGCCACGGTCCACATCGACGGAGAGCCTAAGGATTCCATGATCCAACCGGTGACGAAAATCTTAATTGGAGTGGCTTTTATGACCATCCTGATAACATTGGTTTTGAGGGATCCTCCGCAGTGGATCCAGAAATTAAACATTACTGGAGGTAACTTCCCTCCATGGATCATCGCTCTCGTGATCATAGTTTTTACTCGAATGAGGAAGAGAACCAGGGATTTGCTGAAAAAGTTTGGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

21.38

Weight (kDa)

9.43

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71780
fragaria_vesca FvH4_2g12791
malus_domestica MD05G1036200.v1.1 MD10G1038300.v1.1
prunus_persica Prupe.8G044500_v2.0.a1 Prupe.8G044500_v2.0.a1
pyrus_communis pycom10g02630
rosa_chinensis RchiOBHm_Chr6g0273681
rosa_laevigata RLG00000013600
rosa_multiflora Rmu_sc0021426.1_g000002
rosa_roxburghii Rroxscaffold_7G00195130
rosa_rugosa Rorug06G0077200
rosa_samantha Rh6AG191500 Rh6BG195600 Rh6CG195100 Rh6DG186900
rosa_wichuraiana Rw6G016760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 294
AccII CGCG 1 cut(s) 270
AciI CCGC 2 cut(s) 270, 446
AclWI GGATC 7 cut(s) 274, 364, 434, 447, 448, 461, 503
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 2 cut(s) 202, 299
AflIII ACRYGT 1 cut(s) 159
AgeI ACCGGT 1 cut(s) 376
AgsI TTSAA 3 cut(s) 166, 235, 253
AjnI CCWGG 1 cut(s) 542
AluBI AGCT 1 cut(s) 14
AluI AGCT 1 cut(s) 14
Alw26I GTCTC 1 cut(s) 204
AlwI GGATC 7 cut(s) 274, 364, 434, 447, 448, 461, 503
AoxI GGCC 1 cut(s) 148
ApeKI GCWGC 1 cut(s) 313
AsiGI ACCGGT 1 cut(s) 376
AspS9I GGNCC 4 cut(s) 47, 149, 187, 337
AsuC2I CCSGG 1 cut(s) 28
AsuHPI GGTGA 1 cut(s) 391
AvaII GGWCC 3 cut(s) 47, 187, 337
AxyI CCTNAGG 1 cut(s) 357
BamHI GGATCC 2 cut(s) 439, 453
BauI CACGAG 1 cut(s) 506
BbvI GCAGC 1 cut(s) 300
BccI CCATC 2 cut(s) 320, 422
BceAI ACGGC 2 cut(s) 301, 316
BciT130I CCWGG 1 cut(s) 544
BclI TGATCA 1 cut(s) 510
BcnI CCSGG 1 cut(s) 28
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 1 cut(s) 294
BfuAI ACCTGC 1 cut(s) 294
BisI GCNGC 1 cut(s) 314
BlsI GCNGC 1 cut(s) 315
Bme1390I CCNGG 2 cut(s) 28, 544
Bme18I GGWCC 3 cut(s) 47, 187, 337
BmgT120I GGNCC 4 cut(s) 47, 149, 187, 337
BmiI GGNNCC 7 cut(s) 48, 111, 188, 276, 331, 441, 455
BmrFI CCNGG 2 cut(s) 28, 544
BplI GAGNNNNNCTC 4 cut(s) 201, 233, 427, 459
BpmI CTGGAG 1 cut(s) 495
BpuMI CCSGG 1 cut(s) 28
BsaBI GATNNNNATC 1 cut(s) 235
BsaJI CCNNGG 4 cut(s) 27, 333, 491, 543
BsaWI WCCGGW 2 cut(s) 282, 376
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 299
Bse118I RCCGGY 1 cut(s) 376
Bse1I ACTGG 2 cut(s) 196, 478
Bse21I CCTNAGG 1 cut(s) 357
Bse8I GATNNNNATC 1 cut(s) 235
BseBI CCWGG 1 cut(s) 544
BseDI CCNNGG 4 cut(s) 27, 333, 491, 543
BseGI GGATG 1 cut(s) 414
BseJI GATNNNNATC 1 cut(s) 235
BseLI CCNNNNNNNGG 2 cut(s) 202, 299
BseNI ACTGG 2 cut(s) 196, 478
BseXI GCAGC 1 cut(s) 300
Bsh1236I CGCG 1 cut(s) 270
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 1 cut(s) 150
BshTI ACCGGT 1 cut(s) 376
BsiEI CGRYCG 1 cut(s) 156
BsiSI CCGG 3 cut(s) 27, 283, 377
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 2 cut(s) 202, 299
BsmAI GTCTC 1 cut(s) 204
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 1 cut(s) 150
Bsp143I GATC 7 cut(s) 153, 279, 369, 439, 453, 495, 510
Bsp19I CCATGG 1 cut(s) 491
BspACI CCGC 2 cut(s) 270, 446
BspANI GGCC 1 cut(s) 150
BspFNI CGCG 1 cut(s) 270
BspLI GGNNCC 7 cut(s) 48, 111, 188, 276, 331, 441, 455
BspMI ACCTGC 1 cut(s) 294
BspPI GGATC 7 cut(s) 274, 364, 434, 447, 448, 461, 503
BsrFI RCCGGY 1 cut(s) 376
BsrI ACTGG 2 cut(s) 196, 478
BssAI RCCGGY 1 cut(s) 376
BssECI CCNNGG 4 cut(s) 27, 333, 491, 543
BssMI GATC 7 cut(s) 153, 279, 369, 439, 453, 495, 510
BssSI CACGAG 1 cut(s) 506
BssT1I CCWWGG 1 cut(s) 491
Bst2BI CACGAG 1 cut(s) 506
Bst2UI CCWGG 1 cut(s) 544
Bst4CI ACNGT 1 cut(s) 337
Bst6I CTCTTC 1 cut(s) 530
BstDEI CTNAG 1 cut(s) 357
BstDSI CCRYGG 2 cut(s) 333, 491
BstF5I GGATG 1 cut(s) 414
BstFNI CGCG 1 cut(s) 270
BstKTI GATC 7 cut(s) 156, 282, 372, 442, 456, 498, 513
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 7 cut(s) 153, 279, 369, 439, 453, 495, 510
BstMCI CGRYCG 1 cut(s) 156
BstNI CCWGG 1 cut(s) 544
BstSCI CCNGG 2 cut(s) 26, 542
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 1 cut(s) 300
BstX2I RGATCY 2 cut(s) 439, 453
BstYI RGATCY 2 cut(s) 439, 453
Bsu36I CCTNAGG 1 cut(s) 357
BsuRI GGCC 1 cut(s) 150
BtgI CCRYGG 2 cut(s) 333, 491
BtgZI GCGATG 1 cut(s) 484
BtsCI GGATG 1 cut(s) 414
BtsI GCAGTG 1 cut(s) 455
BtsIMutI CAGTG 1 cut(s) 455
BveI ACCTGC 1 cut(s) 294
Cfr10I RCCGGY 1 cut(s) 376
Cfr13I GGNCC 4 cut(s) 47, 149, 187, 337
Csp6I GTAC 1 cut(s) 226
CspAI ACCGGT 1 cut(s) 376
CviAII CATG 2 cut(s) 367, 492
CviJI RGCY 5 cut(s) 14, 150, 332, 355, 404
CviKI_1 RGCY 5 cut(s) 14, 150, 332, 355, 404
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 1 cut(s) 357
DpnI GATC 7 cut(s) 155, 281, 371, 441, 455, 497, 512
DpnII GATC 7 cut(s) 153, 279, 369, 439, 453, 495, 510
Eam1104I CTCTTC 1 cut(s) 530
EarI CTCTTC 1 cut(s) 530
Eco130I CCWWGG 1 cut(s) 491
Eco47I GGWCC 3 cut(s) 47, 187, 337
Eco81I CCTNAGG 1 cut(s) 357
EcoRII CCWGG 1 cut(s) 542
EcoT14I CCWWGG 1 cut(s) 491
ErhI CCWWGG 1 cut(s) 491
FaeI CATG 2 cut(s) 370, 495
FaiI YATR 6 cut(s) 311, 321, 368, 410, 493, 515
FaqI GGGAC 1 cut(s) 200
FatI CATG 2 cut(s) 366, 491
FauI CCCGC 1 cut(s) 263
FbaI TGATCA 1 cut(s) 510
Fnu4HI GCNGC 1 cut(s) 314
FokI GGATG 1 cut(s) 401
Fsp4HI GCNGC 1 cut(s) 314
FspBI CTAG 1 cut(s) 294
GluI GCNGC 1 cut(s) 314
GsuI CTGGAG 1 cut(s) 495
HaeIII GGCC 1 cut(s) 150
HapII CCGG 3 cut(s) 27, 283, 377
Hin1II CATG 2 cut(s) 370, 495
HinfI GANTC 5 cut(s) 79, 85, 231, 260, 362
HpaII CCGG 3 cut(s) 27, 283, 377
HphI GGTGA 1 cut(s) 391
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 5 cut(s) 58, 72, 84, 209, 279
Hpy188III TCNNGA 5 cut(s) 113, 218, 418, 457, 508
Hpy8I GTNNAC 1 cut(s) 340
Hpy99I CGWCG 2 cut(s) 62, 350
HpyAV CCTTC 1 cut(s) 100
HpyCH4III ACNGT 1 cut(s) 337
HpyCH4IV ACGT 1 cut(s) 161
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 1 cut(s) 161
Hsp92II CATG 2 cut(s) 370, 495
Ksp22I TGATCA 1 cut(s) 510
Kzo9I GATC 7 cut(s) 153, 279, 369, 439, 453, 495, 510
LmnI GCTCC 1 cut(s) 329
Lsp1109I GCAGC 1 cut(s) 300
MaeI CTAG 1 cut(s) 294
MaeII ACGT 1 cut(s) 161
MaeIII GTNAC 2 cut(s) 379, 479
MalI GATC 7 cut(s) 155, 281, 371, 441, 455, 497, 512
MboI GATC 7 cut(s) 153, 279, 369, 439, 453, 495, 510
MboII GAAGA 1 cut(s) 547
MflI RGATCY 2 cut(s) 439, 453
MluCI AATT 2 cut(s) 393, 461
MmeI TCCRAC 4 cut(s) 81, 180, 187, 397
MnlI CCTC 9 cut(s) 31, 93, 173, 196, 429, 453, 470, 498, 525
MseI TTAA 3 cut(s) 288, 392, 464
MspI CCGG 3 cut(s) 27, 283, 377
MspR9I CCNGG 2 cut(s) 28, 544
MvaI CCWGG 1 cut(s) 544
MvnI CGCG 1 cut(s) 270
NciI CCSGG 1 cut(s) 28
NcoI CCATGG 1 cut(s) 491
NdeII GATC 7 cut(s) 153, 279, 369, 439, 453, 495, 510
NlaIII CATG 2 cut(s) 370, 495
NlaIV GGNNCC 7 cut(s) 48, 111, 188, 276, 331, 441, 455
NmuCI GTSAC 1 cut(s) 379
PfeI GAWTC 5 cut(s) 79, 85, 231, 260, 362
PinAI ACCGGT 1 cut(s) 376
PkrI GCNGC 1 cut(s) 315
Ple19I CGATCG 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 542
PspGI CCWGG 1 cut(s) 542
PspN4I GGNNCC 7 cut(s) 48, 111, 188, 276, 331, 441, 455
PspPI GGNCC 4 cut(s) 47, 149, 187, 337
PsuI RGATCY 2 cut(s) 439, 453
PvuI CGATCG 1 cut(s) 156
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
SaqAI TTAA 3 cut(s) 288, 392, 464
SatI GCNGC 1 cut(s) 314
Sau3AI GATC 7 cut(s) 153, 279, 369, 439, 453, 495, 510
Sau96I GGNCC 4 cut(s) 47, 149, 187, 337
ScrFI CCNGG 2 cut(s) 28, 544
SetI ASST 7 cut(s) 16, 111, 164, 184, 207, 308, 481
SinI GGWCC 3 cut(s) 47, 187, 337
Sse9I AATT 2 cut(s) 393, 461
SsiI CCGC 2 cut(s) 270, 446
SspMI CTAG 1 cut(s) 294
StyD4I CCNGG 2 cut(s) 26, 542
StyI CCWWGG 1 cut(s) 491
TaaI ACNGT 1 cut(s) 337
TaiI ACGT 1 cut(s) 164
TaqI TCGA 5 cut(s) 136, 156, 219, 345, 526
TasI AATT 2 cut(s) 393, 461
TfiI GAWTC 5 cut(s) 79, 85, 231, 260, 362
Tru1I TTAA 3 cut(s) 288, 392, 464
Tru9I TTAA 3 cut(s) 288, 392, 464
TscAI CASTG 1 cut(s) 455
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 1 cut(s) 313
Tsp45I GTSAC 1 cut(s) 379
TspGWI ACGGA 2 cut(s) 243, 363
TspRI CASTG 1 cut(s) 455
VpaK11BI GGWCC 3 cut(s) 47, 187, 337
XspI CTAG 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.