MD10G1043000.v1.1

Family of unknown function (DUF5306)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016755
Physical Location & Seq
Reverse (-)
5698460 .. 5699295
836 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1043000.v1.1.491

Sequence Viewer

Length: 369 bp
ATGGAGTACTCACAGTCAAGCAAAGTATTAGCTGATGATGAGGAGGAGTTGATGGAGAAGAGAAGTTACCATATATTTCCATGCCACTTCCTTGAAGAAGCAGTCAGGGCTTTCTTCAAGTGTTTGGGTATTGAGACTAAATCTCAGGAGGACAACGAAAACCCCAAGAGAGCTACTCCTGCTTCAACCCCAGAACAGAATGATGCAGATCCATCACTTCCGACGACGGACGAAGATCCTCCATCTTCATCAGCTACAACAGGAACCACAGACGAAGTTGCTGCAGATACAAGGACAAATTTGAGCGCTCGTGCAAGACCAGGACTAAGCGTAGGGGGAGGTGGGAGAATTAATTCATCAAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF5306

Protein Analysis

123

Amino Acids

13.22

Weight (kDa)

4.54

Isoelectric Point (pI)

63.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pep1_7 PF17232 16 - 57 2.5e-09 Elicitor peptide 1-7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016755)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12531
malus_domestica MD00G1161700.v1.1 MD10G1043000.v1.1
prunus_persica Prupe.8G048500_v2.0.a1
pyrus_communis pycom05g03040
rosa_laevigata RLG00000013646
rosa_roxburghii Rroxscaffold_1G00008980 Rroxscaffold_3G00220660 Rroxscaffold_7G00195720
rosa_rugosa Rorug06G0072300
rosa_samantha Rh6AG187900 Rh6BG191300 Rh6CG190300 Rh6DG181900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 203, 230
AcsI RAATTY 1 cut(s) 298
AfaI GTAC 1 cut(s) 8
AfeI AGCGCT 1 cut(s) 307
AgsI TTSAA 3 cut(s) 95, 118, 186
AjnI CCWGG 1 cut(s) 319
AluBI AGCT 3 cut(s) 32, 173, 254
AluI AGCT 3 cut(s) 32, 173, 254
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 2 cut(s) 203, 230
Aor51HI AGCGCT 1 cut(s) 307
ApeKI GCWGC 1 cut(s) 281
ApoI RAATTY 1 cut(s) 298
AseI ATTAAT 1 cut(s) 351
Asp700I GAANNNNTTC 1 cut(s) 352
AspLEI GCGC 1 cut(s) 308
BarI GAAGNNNNNNTAC 2 cut(s) 50, 82
BauI CACGAG 1 cut(s) 309
BbvI GCAGC 1 cut(s) 268
BccI CCATC 3 cut(s) 46, 220, 250
BcgI CGANNNNNNTGC 2 cut(s) 263, 297
BciT130I CCWGG 1 cut(s) 321
BcoDI GTCTC 1 cut(s) 128
BfmI CTRYAG 1 cut(s) 282
BfoI RGCGCY 1 cut(s) 309
BisI GCNGC 1 cut(s) 282
BlsI GCNGC 1 cut(s) 283
BmcAI AGTACT 1 cut(s) 8
Bme1390I CCNGG 1 cut(s) 321
BmiI GGNNCC 1 cut(s) 265
BmrFI CCNGG 1 cut(s) 321
BmsI GCATC 1 cut(s) 193
BplI GAGNNNNNCTC 2 cut(s) 160, 192
BsaBI GATNNNNATC 1 cut(s) 207
Bse8I GATNNNNATC 1 cut(s) 207
BseBI CCWGG 1 cut(s) 321
BseJI GATNNNNATC 1 cut(s) 207
BseMII CTCAG 1 cut(s) 158
BseRI GAGGAG 2 cut(s) 56, 59
BseXI GCAGC 1 cut(s) 268
BsmAI GTCTC 1 cut(s) 128
Bsp143I GATC 2 cut(s) 208, 235
BspCNI CTCAG 1 cut(s) 157
BspLI GGNNCC 1 cut(s) 265
BspMAI CTGCAG 1 cut(s) 286
BspPI GGATC 2 cut(s) 203, 230
BssMI GATC 2 cut(s) 208, 235
BssSI CACGAG 1 cut(s) 309
Bst2BI CACGAG 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 1 cut(s) 15
Bst6I CTCTTC 1 cut(s) 53
BstDEI CTNAG 2 cut(s) 144, 326
BstH2I RGCGCY 1 cut(s) 309
BstHHI GCGC 1 cut(s) 308
BstKTI GATC 2 cut(s) 211, 238
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 2 cut(s) 208, 235
BstMWI GCNNNNNNNGC 2 cut(s) 107, 179
BstNI CCWGG 1 cut(s) 321
BstSCI CCNGG 1 cut(s) 319
BstSFI CTRYAG 1 cut(s) 282
BstV1I GCAGC 1 cut(s) 268
BstX2I RGATCY 2 cut(s) 208, 235
BstYI RGATCY 2 cut(s) 208, 235
CfoI GCGC 1 cut(s) 308
Csp6I GTAC 1 cut(s) 7
CviAII CATG 1 cut(s) 81
CviJI RGCY 4 cut(s) 32, 110, 173, 254
CviKI_1 RGCY 4 cut(s) 32, 110, 173, 254
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 2 cut(s) 144, 326
DpnI GATC 2 cut(s) 210, 237
DpnII GATC 2 cut(s) 208, 235
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
Eco47III AGCGCT 1 cut(s) 307
EcoRII CCWGG 1 cut(s) 319
FaeI CATG 1 cut(s) 84
FaiI YATR 3 cut(s) 72, 74, 82
FatI CATG 1 cut(s) 80
Fnu4HI GCNGC 1 cut(s) 282
Fsp4HI GCNGC 1 cut(s) 282
GlaI GCGC 1 cut(s) 307
GluI GCNGC 1 cut(s) 282
HaeII RGCGCY 1 cut(s) 309
HhaI GCGC 1 cut(s) 308
Hin1II CATG 1 cut(s) 84
Hin6I GCGC 1 cut(s) 306
HinP1I GCGC 1 cut(s) 306
Hpy188I TCNGA 1 cut(s) 222
Hpy188III TCNNGA 1 cut(s) 146
Hpy99I CGWCG 2 cut(s) 226, 229
HpyCH4III ACNGT 1 cut(s) 15
HpyCH4V TGCA 3 cut(s) 206, 284, 314
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 179
HpyF3I CTNAG 2 cut(s) 144, 326
Hsp92II CATG 1 cut(s) 84
HspAI GCGC 1 cut(s) 306
Kzo9I GATC 2 cut(s) 208, 235
LpnPI CCDG 7 cut(s) 91, 131, 192, 204, 246, 306, 333
Lsp1109I GCAGC 1 cut(s) 268
LweI GCATC 1 cut(s) 193
MaeIII GTNAC 1 cut(s) 65
MalI GATC 2 cut(s) 210, 237
MboI GATC 2 cut(s) 208, 235
MboII GAAGA 5 cut(s) 70, 106, 107, 237, 245
MflI RGATCY 2 cut(s) 208, 235
MluCI AATT 4 cut(s) 298, 348, 352, 364
MmeI TCCRAC 1 cut(s) 245
MnlI CCTC 5 cut(s) 34, 37, 142, 249, 332
MroXI GAANNNNTTC 1 cut(s) 352
MseI TTAA 2 cut(s) 351, 367
MspR9I CCNGG 1 cut(s) 321
MvaI CCWGG 1 cut(s) 321
MwoI GCNNNNNNNGC 2 cut(s) 107, 179
NdeII GATC 2 cut(s) 208, 235
NlaIII CATG 1 cut(s) 84
NlaIV GGNNCC 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 352
PkrI GCNGC 1 cut(s) 283
PshBI ATTAAT 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 319
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 1 cut(s) 265
PstI CTGCAG 1 cut(s) 286
PsuI RGATCY 2 cut(s) 208, 235
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SaqAI TTAA 2 cut(s) 351, 367
SatI GCNGC 1 cut(s) 282
Sau3AI GATC 2 cut(s) 208, 235
ScaI AGTACT 1 cut(s) 8
ScrFI CCNGG 1 cut(s) 321
SetI ASST 4 cut(s) 34, 175, 256, 343
SfaNI GCATC 1 cut(s) 193
SfcI CTRYAG 1 cut(s) 282
Sse9I AATT 4 cut(s) 298, 348, 352, 364
StyD4I CCNGG 1 cut(s) 319
TaaI ACNGT 1 cut(s) 15
TasI AATT 4 cut(s) 298, 348, 352, 364
TatI WGTACW 1 cut(s) 6
Tru1I TTAA 2 cut(s) 351, 367
Tru9I TTAA 2 cut(s) 351, 367
TseI GCWGC 1 cut(s) 281
TspDTI ATGAA 2 cut(s) 237, 345
TspGWI ACGGA 1 cut(s) 242
VspI ATTAAT 1 cut(s) 351
XapI RAATTY 1 cut(s) 298
XmnI GAANNNNTTC 1 cut(s) 352
ZrmI AGTACT 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.