MD10G1053500.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0017372
Physical Location & Seq
Forward (+)
7192555 .. 7195741
3187 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1053500.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGGACCCCTCCCTCCCTTCCTTTCACCGCCACCCCCACTTCCACCTTATCTCTTACATTTCTTGCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATAGCTAGTTTAATGGCTGCAACTGATCTAATGGTTAAGGTTAACTCGTCACAAGTTTGCCGCGATCATCCACCACCCAAACTGACCACCATAAAAAAGCTTATTGCGGAATCACAAGACACCAACGGCAACTTCATTGTTCCTCCCACTTATACCTACACAAACAATAATAATCCCCACGATGATCAGAAAGCTTTCGAAGAAGATACAGAAGCAGCAACCATTCCGACCGTTGACTTCTCTCTTCTCACAACATCTGCTACTCCTGAACAGCGCTCTAAAGCCATCAACGACCTCGGCAAAGCCTGCCAGGACTGGGGCTTCTTCATGGTGATCAATCATGGTGTGCCAGAGAGTCTGATGAAGGTAATCTTGGATGGTTGTGAAGGCTTTTTTGATCTGACGGAGGAGGAGAAGCAGGAGTTTGAAGGGAAGCATGTGTTGGACCCCATCAGATGCGGGACCAGCTTCAATACCTCCGTGGAGAATGTTCGGTTTTGGAGGGATTTTCTCAAGGTTTTTGTATATCCTGAGTTTCACTTCCCCTCTAAACCTGCTGGCTTCAGTGAGCTTGCATCAGAGTATTGCAAAAGAACAAGAGAAGTGACAAGGGAGCTACTGAAAGGAATATCAGAAAGCCTGGGGTTGGAGGCCACAGACATAGACAAGGCCTTGGATGTGGAATCAGCTGGCTTGCAAATCTTCATTGCAAACCTGTATCCTCCTTGTCCACAGCCTGAACTTGCAATGGGGATGCCTCCTCATTCTGATCATGGCCTCTTGACCCTTCTCATGCAGAATGGGATCAGTGGCCTTCAAGTGCAACACAAGGGTAAATGGGTCAACGTCAATGCTGCACCCTACTCCTTCCTTGTCAACACTGGCGATCACCTTGAGATATTTAGCAATGGAAAGTACAGGAGCAACATTCACAGGGCAGTGTTGAACAACAAATCAACAAGAATATCGGTAGCCACTCCGACTGGCCCATCACTTGAAACAGTAGTTACACCTACACCAAAGTTGGTAGACACTGCAAAGCATCCGGCAGCAGCATATATTGGCATGAAGTACAAGGACTACTTGGAGCTTCAACAAGGCAGCGTACTTGATGGGAAAGTCATCTTGGATCGTGTACGTGCGGGGGTTTCAAACTCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

422

Amino Acids

46.61

Weight (kDa)

5.76

Isoelectric Point (pI)

31.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 109 - 211 8.4e-23 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 267 - 361 8.8e-29 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017372)

Species Orthologous Gene IDs
malus_domestica MD10G1053500.v1.1
prunus_persica Prupe.8G066900_v2.0.a1
pyrus_communis pycom10g04050
rosa_chinensis RchiOBHm_Chr6g0271361
rosa_laevigata RLG00000013727
rosa_multiflora Rmu_sc0004866.1_g000028
rosa_roxburghii Rroxscaffold_7G00197070
rosa_rugosa Rorug06G0060500
rosa_samantha Rh6AG179600 Rh6BG182600 Rh6CG179500 Rh6DG171200
rosa_wichuraiana Rw6G015440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 664
AccI GTMKAC 1 cut(s) 1131
AccII CGCG 1 cut(s) 165
AciI CCGC 5 cut(s) 28, 163, 209, 561, 1244
AclWI GGATC 2 cut(s) 914, 1239
AcuI CTGAAG 1 cut(s) 649
AfaI GTAC 4 cut(s) 1019, 1175, 1209, 1239
AfeI AGCGCT 1 cut(s) 377
AfiI CCNNNNNNNGG 2 cut(s) 418, 748
AgsI TTSAA 7 cut(s) 530, 574, 920, 1048, 1100, 1196, 1254
AjnI CCWGG 2 cut(s) 411, 741
AjuI GAANNNNNNNTTGG 8 cut(s) 218, 250, 458, 490, 527, 559, 1211, 1243
AluBI AGCT 8 cut(s) 107, 202, 296, 570, 673, 718, 791, 1192
AluI AGCT 8 cut(s) 107, 202, 296, 570, 673, 718, 791, 1192
AlwI GGATC 2 cut(s) 914, 1239
Aor51HI AGCGCT 1 cut(s) 377
AoxI GGCC 5 cut(s) 753, 771, 877, 913, 1087
ApeKI GCWGC 6 cut(s) 119, 317, 956, 1151, 1154, 1203
Asp700I GAANNNNTTC 1 cut(s) 296
AspLEI GCGC 1 cut(s) 378
AspS9I GGNCC 4 cut(s) 4, 547, 564, 1088
AsuHPI GGTGA 3 cut(s) 17, 445, 983
AsuII TTCGAA 1 cut(s) 300
AvaII GGWCC 3 cut(s) 4, 547, 564
BbvI GCAGC 6 cut(s) 106, 329, 943, 1163, 1166, 1215
BccI CCATC 5 cut(s) 395, 473, 560, 1099, 1208
BceAI ACGGC 1 cut(s) 244
BciT130I CCWGG 2 cut(s) 413, 743
BciVI GTATCC 1 cut(s) 831
BclI TGATCA 3 cut(s) 286, 435, 871
BfaI CTAG 1 cut(s) 108
BfmI CTRYAG 1 cut(s) 102
BfoI RGCGCY 1 cut(s) 379
BfuAI ACCTGC 1 cut(s) 664
BfuI GTATCC 1 cut(s) 831
BisI GCNGC 7 cut(s) 120, 163, 318, 957, 1152, 1155, 1204
BlsI GCNGC 7 cut(s) 121, 164, 319, 958, 1153, 1156, 1205
Bme1390I CCNGG 2 cut(s) 413, 743
Bme18I GGWCC 3 cut(s) 4, 547, 564
BmgT120I GGNCC 4 cut(s) 4, 547, 564, 1088
BmiI GGNNCC 3 cut(s) 6, 549, 565
BmrFI CCNGG 2 cut(s) 413, 743
BmrI ACTGGG 1 cut(s) 427
BmsI GCATC 4 cut(s) 548, 686, 846, 1153
BmuI ACTGGG 1 cut(s) 427
Bpu14I TTCGAA 1 cut(s) 300
BpuEI CTTGAG 2 cut(s) 599, 1016
BsaAI YACGTR 1 cut(s) 1241
BsaJI CCNNGG 4 cut(s) 397, 582, 742, 774
Bsc4I CCNNNNNNNGG 2 cut(s) 418, 748
Bse1I ACTGG 3 cut(s) 422, 988, 1090
Bse3DI GCAATG 3 cut(s) 807, 855, 1015
BseBI CCWGG 2 cut(s) 413, 743
BseDI CCNNGG 4 cut(s) 397, 582, 742, 774
BseGI GGATG 5 cut(s) 169, 484, 784, 861, 1144
BseLI CCNNNNNNNGG 2 cut(s) 418, 748
BseMI GCAATG 3 cut(s) 807, 855, 1015
BseMII CTCAG 1 cut(s) 624
BseNI ACTGG 3 cut(s) 422, 988, 1090
BseRI GAGGAG 3 cut(s) 524, 527, 852
BseXI GCAGC 6 cut(s) 106, 329, 943, 1163, 1166, 1215
BsgI GTGCAG 1 cut(s) 942
Bsh1236I CGCG 1 cut(s) 165
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 5 cut(s) 755, 773, 879, 915, 1089
BsiEI CGRYCG 1 cut(s) 333
BsiSI CCGG 1 cut(s) 1148
BslFI GGGAC 1 cut(s) 577
BslI CCNNNNNNNGG 2 cut(s) 418, 748
BsmFI GGGAC 1 cut(s) 577
BsnI GGCC 5 cut(s) 755, 773, 879, 915, 1089
Bsp119I TTCGAA 1 cut(s) 300
Bsp143I GATC 9 cut(s) 127, 166, 286, 435, 499, 871, 906, 988, 1231
BspACI CCGC 5 cut(s) 28, 163, 209, 561, 1244
BspANI GGCC 5 cut(s) 755, 773, 879, 915, 1089
BspCNI CTCAG 1 cut(s) 625
BspFNI CGCG 1 cut(s) 165
BspLI GGNNCC 3 cut(s) 6, 549, 565
BspMI ACCTGC 1 cut(s) 664
BspPI GGATC 2 cut(s) 914, 1239
BspT104I TTCGAA 1 cut(s) 300
BsrDI GCAATG 3 cut(s) 807, 855, 1015
BsrI ACTGG 3 cut(s) 422, 988, 1090
BssECI CCNNGG 4 cut(s) 397, 582, 742, 774
BssMI GATC 9 cut(s) 127, 166, 286, 435, 499, 871, 906, 988, 1231
BssT1I CCWWGG 1 cut(s) 774
Bst2UI CCWGG 2 cut(s) 413, 743
Bst4CI ACNGT 2 cut(s) 334, 1105
Bst6I CTCTTC 1 cut(s) 351
BstAPI GCANNNNNTGC 1 cut(s) 408
BstBAI YACGTR 1 cut(s) 1241
BstBI TTCGAA 1 cut(s) 300
BstC8I GCNNGC 5 cut(s) 409, 661, 675, 793, 797
BstDEI CTNAG 1 cut(s) 633
BstDSI CCRYGG 1 cut(s) 582
BstF5I GGATG 5 cut(s) 169, 484, 784, 861, 1144
BstFNI CGCG 1 cut(s) 165
BstH2I RGCGCY 1 cut(s) 379
BstHHI GCGC 1 cut(s) 378
BstKTI GATC 9 cut(s) 130, 169, 289, 438, 502, 874, 909, 991, 1234
BstMBI GATC 9 cut(s) 127, 166, 286, 435, 499, 871, 906, 988, 1231
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 2 cut(s) 408, 567
BstNI CCWGG 2 cut(s) 413, 743
BstNSI RCATGY 1 cut(s) 542
BstSCI CCNGG 2 cut(s) 411, 741
BstSFI CTRYAG 1 cut(s) 102
BstUI CGCG 1 cut(s) 165
BstV1I GCAGC 6 cut(s) 106, 329, 943, 1163, 1166, 1215
BsuI GTATCC 1 cut(s) 831
BsuRI GGCC 5 cut(s) 755, 773, 879, 915, 1089
BtgI CCRYGG 1 cut(s) 582
BtsCI GGATG 5 cut(s) 169, 484, 784, 861, 1144
BtsI GCAGTG 2 cut(s) 1047, 1134
BtsIMutI CAGTG 5 cut(s) 673, 916, 981, 1047, 1134
BveI ACCTGC 1 cut(s) 664
Cac8I GCNNGC 5 cut(s) 409, 661, 675, 793, 797
CfoI GCGC 1 cut(s) 378
Cfr13I GGNCC 4 cut(s) 4, 547, 564, 1088
Csp6I GTAC 4 cut(s) 1018, 1174, 1208, 1238
CviAII CATG 6 cut(s) 430, 443, 539, 875, 895, 1168
CviQI GTAC 4 cut(s) 1018, 1174, 1208, 1238
DdeI CTNAG 1 cut(s) 633
DpnI GATC 9 cut(s) 129, 168, 288, 437, 501, 873, 908, 990, 1233
DpnII GATC 9 cut(s) 127, 166, 286, 435, 499, 871, 906, 988, 1231
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
Eco130I CCWWGG 1 cut(s) 774
Eco147I AGGCCT 1 cut(s) 773
Eco47I GGWCC 3 cut(s) 4, 547, 564
Eco47III AGCGCT 1 cut(s) 377
Eco57I CTGAAG 1 cut(s) 649
EcoRII CCWGG 2 cut(s) 411, 741
EcoT14I CCWWGG 1 cut(s) 774
ErhI CCWWGG 1 cut(s) 774
FaeI CATG 6 cut(s) 433, 446, 542, 878, 898, 1171
FalI AAGNNNNNCTT 4 cut(s) 458, 490, 1169, 1201
FaqI GGGAC 1 cut(s) 577
FatI CATG 6 cut(s) 429, 442, 538, 874, 894, 1167
FauI CCCGC 2 cut(s) 554, 1237
FbaI TGATCA 3 cut(s) 286, 435, 871
FblI GTMKAC 1 cut(s) 1131
Fnu4HI GCNGC 7 cut(s) 120, 163, 318, 957, 1152, 1155, 1204
FokI GGATG 5 cut(s) 156, 491, 791, 868, 1131
Fsp4HI GCNGC 7 cut(s) 120, 163, 318, 957, 1152, 1155, 1204
FspBI CTAG 1 cut(s) 108
GlaI GCGC 1 cut(s) 377
GluI GCNGC 7 cut(s) 120, 163, 318, 957, 1152, 1155, 1204
HaeII RGCGCY 1 cut(s) 379
HaeIII GGCC 5 cut(s) 755, 773, 879, 915, 1089
HapII CCGG 1 cut(s) 1148
HhaI GCGC 1 cut(s) 378
Hin1II CATG 6 cut(s) 433, 446, 542, 878, 898, 1171
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HincII GTYRAC 4 cut(s) 145, 337, 946, 979
HindII GTYRAC 4 cut(s) 145, 337, 946, 979
HindIII AAGCTT 2 cut(s) 200, 294
HinfI GANTC 3 cut(s) 212, 457, 785
HpaI GTTAAC 1 cut(s) 145
HpaII CCGG 1 cut(s) 1148
HphI GGTGA 3 cut(s) 17, 445, 983
Hpy166II GTNNAC 7 cut(s) 145, 337, 833, 946, 979, 1132, 1238
Hpy188III TCNNGA 3 cut(s) 368, 632, 883
Hpy8I GTNNAC 7 cut(s) 145, 337, 833, 946, 979, 1132, 1238
HpyAV CCTTC 7 cut(s) 27, 460, 482, 524, 899, 926, 979
HpyCH4III ACNGT 2 cut(s) 334, 1105
HpyCH4IV ACGT 2 cut(s) 948, 1240
HpyF10VI GCNNNNNNNGC 2 cut(s) 408, 567
HpyF3I CTNAG 1 cut(s) 633
HpySE526I ACGT 2 cut(s) 948, 1240
Hsp92II CATG 6 cut(s) 433, 446, 542, 878, 898, 1171
HspAI GCGC 1 cut(s) 376
Ksp22I TGATCA 3 cut(s) 286, 435, 871
KspAI GTTAAC 1 cut(s) 145
Kzo9I GATC 9 cut(s) 127, 166, 286, 435, 499, 871, 906, 988, 1231
LmnI GCTCC 3 cut(s) 715, 1023, 1189
Lsp1109I GCAGC 6 cut(s) 106, 329, 943, 1163, 1166, 1215
LweI GCATC 4 cut(s) 548, 686, 846, 1153
MaeI CTAG 1 cut(s) 108
MaeII ACGT 2 cut(s) 948, 1240
MaeIII GTNAC 3 cut(s) 150, 706, 1108
MalI GATC 9 cut(s) 129, 168, 288, 437, 501, 873, 908, 990, 1233
MboI GATC 9 cut(s) 127, 166, 286, 435, 499, 871, 906, 988, 1231
MboII GAAGA 5 cut(s) 314, 317, 338, 418, 796
MlyI GAGTC 1 cut(s) 466
MmeI TCCRAC 4 cut(s) 353, 525, 729, 1106
MroXI GAANNNNTTC 1 cut(s) 296
MseI TTAA 3 cut(s) 113, 138, 144
MspA1I CMGCKG 1 cut(s) 791
MspI CCGG 1 cut(s) 1148
MspR9I CCNGG 2 cut(s) 413, 743
MvaI CCWGG 2 cut(s) 413, 743
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 2 cut(s) 408, 567
NdeII GATC 9 cut(s) 127, 166, 286, 435, 499, 871, 906, 988, 1231
NlaIII CATG 6 cut(s) 433, 446, 542, 878, 898, 1171
NlaIV GGNNCC 3 cut(s) 6, 549, 565
NmeAIII GCCGAG 1 cut(s) 378
NmuCI GTSAC 2 cut(s) 150, 706
NspI RCATGY 1 cut(s) 542
NspV TTCGAA 1 cut(s) 300
PceI AGGCCT 1 cut(s) 773
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 2 cut(s) 212, 785
PkrI GCNGC 7 cut(s) 121, 164, 319, 958, 1153, 1156, 1205
PleI GAGTC 1 cut(s) 465
PpsI GAGTC 1 cut(s) 465
Ppu21I YACGTR 1 cut(s) 1241
Psp6I CCWGG 2 cut(s) 411, 741
PspGI CCWGG 2 cut(s) 411, 741
PspN4I GGNNCC 3 cut(s) 6, 549, 565
PspPI GGNCC 4 cut(s) 4, 547, 564, 1088
PvuII CAGCTG 1 cut(s) 791
RsaI GTAC 4 cut(s) 1019, 1175, 1209, 1239
RsaNI GTAC 4 cut(s) 1018, 1174, 1208, 1238
SaqAI TTAA 3 cut(s) 113, 138, 144
SatI GCNGC 7 cut(s) 120, 163, 318, 957, 1152, 1155, 1204
Sau3AI GATC 9 cut(s) 127, 166, 286, 435, 499, 871, 906, 988, 1231
Sau96I GGNCC 4 cut(s) 4, 547, 564, 1088
SchI GAGTC 1 cut(s) 466
ScrFI CCNGG 2 cut(s) 413, 743
SfaNI GCATC 4 cut(s) 548, 686, 846, 1153
SfcI CTRYAG 1 cut(s) 102
SfuI TTCGAA 1 cut(s) 300
SinI GGWCC 3 cut(s) 4, 547, 564
SmlI CTYRAG 2 cut(s) 614, 995
SmoI CTYRAG 2 cut(s) 614, 995
SseBI AGGCCT 1 cut(s) 773
SsiI CCGC 5 cut(s) 28, 163, 209, 561, 1244
SspMI CTAG 1 cut(s) 108
StuI AGGCCT 1 cut(s) 773
StyD4I CCNGG 2 cut(s) 411, 741
StyI CCWWGG 1 cut(s) 774
TaaI ACNGT 2 cut(s) 334, 1105
TaiI ACGT 2 cut(s) 951, 1243
TaqI TCGA 1 cut(s) 300
TatI WGTACW 2 cut(s) 1017, 1173
TauI GCSGC 1 cut(s) 165
TfiI GAWTC 2 cut(s) 212, 785
Tru1I TTAA 3 cut(s) 113, 138, 144
Tru9I TTAA 3 cut(s) 113, 138, 144
TscAI CASTG 5 cut(s) 673, 916, 988, 1047, 1141
TseFI GTSAC 2 cut(s) 150, 706
TseI GCWGC 6 cut(s) 119, 317, 956, 1151, 1154, 1203
Tsp45I GTSAC 2 cut(s) 150, 706
TspDTI ATGAA 5 cut(s) 226, 418, 479, 796, 1184
TspGWI ACGGA 2 cut(s) 521, 571
TspRI CASTG 5 cut(s) 673, 916, 988, 1047, 1141
VpaK11BI GGWCC 3 cut(s) 4, 547, 564
XceI RCATGY 1 cut(s) 542
XmiI GTMKAC 1 cut(s) 1131
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.