Rroxscaffold_7G00197070

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0017372
Physical Location & Seq
Forward (+)
40889791 .. 40891799
2009 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00197070.1

Sequence Viewer

Length: 1023 bp
ATGGCTGCAGCAACGGATCCAGCAGTTACACAGTACTCACATACTGCTCTACCGCCAATACTGACCAGCGTCAAGAAGCTTTCAGAGTCATCACAAGTCACTTCTATTCCTTCGACTTATACATACCCCGTTAATCCTCAAGATGAGTCAGGTTTGATAGATTACGATTCAAAAATCCCCGTCGTTGACTTCTCTCTTCTCACATCTGGTACTCCGGAGCAGAGGTCCAAAGCCATCAGCGACCTTGGTAAAGCATGCGAGGACTGGGGCTTTTTCCTGGTGATCAATCATGGTGTTCCTGAGGAGCTGATGAAAGCGGTCATGGATGGAAGTTTAAGATTTTTTGATCTGAGCGAGGAGGAGAAGCACGAGTATCAAGGGAAGCATGTATTGGACCCGATAAGGTGTGGCACCAGCTTCAATGCTTCTGTGGATAAGGTCTTGTTTTGGAGAGATTTTCTCAAGGTTTTTGTATACCCTGAGTTTCACTTCCCAGATAAACCCGCTGGCTTCAGTCAACTTTCATCCGAGTATTGCAAGAGAACAAGAGAAGTTACAAGGGAACTACTAAAAGGAATATCACTGAGCTTAGGATTGGAAGATGACTACTTAGATACGGCTTTAAATGTGAAATCCGAAGGTTTACAGATCTTCATTGCAAACTTGTATCCTCCTTGCCCACAGCCAGAGCTTGCACTGGGCATGCCCCCTCATTCTGATCATGGCCTTCTCACCCTCCTCATTCAAAATGGAATTAATGGCCTTCAAGTGCAACACAATGGTAAGTGGGTCAATGTCAACGCCATACCCAACTCCTTTCTTGTTAACACTGGCGATCACCTTGAGATCTTTAGCAATGGGAAGTACAGGAGCAACATACATCGAGCAACGCTAAACAACAAAGGTACAAGAGTATCTATCACCGTTCCAACCGGTCCATCTCTAGATGTAGTTGTGACCTCCGGCATCAAAGTTGGTAGAAAATGGAAATCATCCACCAGCATATGTTGGGATGAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

340

Amino Acids

37.66

Weight (kDa)

5.7

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 59 - 161 2.3e-25 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 216 - 310 1.2e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017372)

Species Orthologous Gene IDs
malus_domestica MD10G1053500.v1.1
prunus_persica Prupe.8G066900_v2.0.a1
pyrus_communis pycom10g04050
rosa_chinensis RchiOBHm_Chr6g0271361
rosa_laevigata RLG00000013727
rosa_multiflora Rmu_sc0004866.1_g000028
rosa_roxburghii Rroxscaffold_7G00197070
rosa_rugosa Rorug06G0060500
rosa_samantha Rh6AG179600 Rh6BG182600 Rh6CG179500 Rh6DG171200
rosa_wichuraiana Rw6G015440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 410
AccI GTMKAC 1 cut(s) 474
AccIII TCCGGA 1 cut(s) 214
AciI CCGC 3 cut(s) 53, 317, 504
AclWI GGATC 2 cut(s) 11, 24
AcuI CTGAAG 1 cut(s) 496
AfaI GTAC 4 cut(s) 35, 211, 866, 907
AgeI ACCGGT 1 cut(s) 932
AgsI TTSAA 4 cut(s) 171, 421, 746, 767
AjnI CCWGG 1 cut(s) 276
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
AluBI AGCT 5 cut(s) 79, 307, 417, 588, 691
AluI AGCT 5 cut(s) 79, 307, 417, 588, 691
AlwI GGATC 2 cut(s) 11, 24
Aor13HI TCCGGA 1 cut(s) 214
AoxI GGCC 2 cut(s) 724, 760
ApeKI GCWGC 2 cut(s) 5, 8
AseI ATTAAT 1 cut(s) 756
AsiGI ACCGGT 1 cut(s) 932
AspS9I GGNCC 3 cut(s) 225, 394, 935
AsuHPI GGTGA 4 cut(s) 292, 724, 830, 913
AvaII GGWCC 3 cut(s) 225, 394, 935
AxyI CCTNAGG 1 cut(s) 300
BaeI ACNNNNGTAYC 4 cut(s) 897, 897, 930, 930
BamHI GGATCC 1 cut(s) 16
BanI GGYRCC 1 cut(s) 410
BauI CACGAG 1 cut(s) 368
BbvI GCAGC 1 cut(s) 20
BccI CCATC 3 cut(s) 242, 320, 946
BceAI ACGGC 1 cut(s) 633
BciT130I CCWGG 1 cut(s) 278
BciVI GTATCC 1 cut(s) 678
BclI TGATCA 2 cut(s) 282, 718
BfaI CTAG 1 cut(s) 944
BfmI CTRYAG 1 cut(s) 6
BfuI GTATCC 1 cut(s) 678
BglII AGATCT 2 cut(s) 648, 846
BisI GCNGC 2 cut(s) 6, 9
BlsI GCNGC 2 cut(s) 7, 10
BmcAI AGTACT 1 cut(s) 35
Bme1390I CCNGG 1 cut(s) 278
Bme18I GGWCC 3 cut(s) 225, 394, 935
BmgT120I GGNCC 3 cut(s) 225, 394, 935
BmiI GGNNCC 3 cut(s) 18, 396, 412
BmrFI CCNGG 1 cut(s) 278
BmrI ACTGGG 2 cut(s) 274, 707
BmsI GCATC 1 cut(s) 975
BmuI ACTGGG 2 cut(s) 274, 707
BoxI GACNNNNGTC 1 cut(s) 68
BplI GAGNNNNNCTC 2 cut(s) 444, 476
Bpu10I CCTNAGC 1 cut(s) 589
BpuEI CTTGAG 3 cut(s) 123, 446, 863
BsaJI CCNNGG 1 cut(s) 244
BsaWI WCCGGW 2 cut(s) 214, 932
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bse118I RCCGGY 1 cut(s) 932
Bse1I ACTGG 3 cut(s) 269, 702, 835
Bse21I CCTNAGG 1 cut(s) 300
Bse3DI GCAATG 2 cut(s) 654, 862
BseAI TCCGGA 1 cut(s) 214
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 1 cut(s) 244
BseGI GGATG 4 cut(s) 331, 524, 992, 1018
BseMI GCAATG 2 cut(s) 654, 862
BseMII CTCAG 4 cut(s) 291, 341, 471, 575
BseNI ACTGG 3 cut(s) 269, 702, 835
BseRI GAGGAG 4 cut(s) 317, 371, 374, 728
BseXI GCAGC 1 cut(s) 20
BshFI GGCC 2 cut(s) 726, 762
BshNI GGYRCC 1 cut(s) 410
BshTI ACCGGT 1 cut(s) 932
BsiSI CCGG 3 cut(s) 215, 933, 963
BsnI GGCC 2 cut(s) 726, 762
Bsp13I TCCGGA 1 cut(s) 214
Bsp143I GATC 7 cut(s) 16, 282, 346, 648, 718, 835, 846
BspACI CCGC 3 cut(s) 53, 317, 504
BspANI GGCC 2 cut(s) 726, 762
BspCNI CTCAG 4 cut(s) 292, 342, 472, 576
BspEI TCCGGA 1 cut(s) 214
BspLI GGNNCC 3 cut(s) 18, 396, 412
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 2 cut(s) 11, 24
BspT107I GGYRCC 1 cut(s) 410
BsrDI GCAATG 2 cut(s) 654, 862
BsrFI RCCGGY 1 cut(s) 932
BsrI ACTGG 3 cut(s) 269, 702, 835
BssAI RCCGGY 1 cut(s) 932
BssECI CCNNGG 1 cut(s) 244
BssMI GATC 7 cut(s) 16, 282, 346, 648, 718, 835, 846
BssNAI GTATAC 1 cut(s) 475
BssSI CACGAG 1 cut(s) 368
BssT1I CCWWGG 1 cut(s) 244
Bst1107I GTATAC 1 cut(s) 475
Bst2BI CACGAG 1 cut(s) 368
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 2 cut(s) 33, 925
Bst6I CTCTTC 1 cut(s) 201
BstC8I GCNNGC 4 cut(s) 256, 508, 693, 704
BstDEI CTNAG 6 cut(s) 300, 350, 480, 584, 589, 610
BstF5I GGATG 4 cut(s) 331, 524, 992, 1018
BstKTI GATC 7 cut(s) 19, 285, 349, 651, 721, 838, 849
BstMBI GATC 7 cut(s) 16, 282, 346, 648, 718, 835, 846
BstNI CCWGG 1 cut(s) 278
BstNSI RCATGY 3 cut(s) 258, 389, 706
BstPAI GACNNNNGTC 1 cut(s) 68
BstSCI CCNGG 1 cut(s) 276
BstSFI CTRYAG 1 cut(s) 6
BstV1I GCAGC 1 cut(s) 20
BstX2I RGATCY 3 cut(s) 16, 648, 846
BstYI RGATCY 3 cut(s) 16, 648, 846
BstZ17I GTATAC 1 cut(s) 475
Bsu36I CCTNAGG 1 cut(s) 300
BsuI GTATCC 1 cut(s) 678
BsuRI GGCC 2 cut(s) 726, 762
BtsCI GGATG 4 cut(s) 331, 524, 992, 1018
BtsIMutI CAGTG 3 cut(s) 581, 695, 828
Cac8I GCNNGC 4 cut(s) 256, 508, 693, 704
Cfr10I RCCGGY 1 cut(s) 932
Cfr13I GGNCC 3 cut(s) 225, 394, 935
CseI GACGC 1 cut(s) 58
Csp6I GTAC 4 cut(s) 34, 210, 865, 906
CspAI ACCGGT 1 cut(s) 932
CviAII CATG 6 cut(s) 255, 290, 322, 386, 703, 722
CviQI GTAC 4 cut(s) 34, 210, 865, 906
DdeI CTNAG 6 cut(s) 300, 350, 480, 584, 589, 610
DpnI GATC 7 cut(s) 18, 284, 348, 650, 720, 837, 848
DpnII GATC 7 cut(s) 16, 282, 346, 648, 718, 835, 846
DraI TTTAAA 1 cut(s) 624
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco130I CCWWGG 1 cut(s) 244
Eco47I GGWCC 3 cut(s) 225, 394, 935
Eco57I CTGAAG 1 cut(s) 496
Eco81I CCTNAGG 1 cut(s) 300
EcoRII CCWGG 1 cut(s) 276
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 6 cut(s) 258, 293, 325, 389, 706, 725
FatI CATG 6 cut(s) 254, 289, 321, 385, 702, 721
FauI CCCGC 1 cut(s) 511
FauNDI CATATG 1 cut(s) 1004
FbaI TGATCA 2 cut(s) 282, 718
FblI GTMKAC 1 cut(s) 474
Fnu4HI GCNGC 2 cut(s) 6, 9
FokI GGATG 3 cut(s) 338, 511, 979
Fsp4HI GCNGC 2 cut(s) 6, 9
FspBI CTAG 1 cut(s) 944
GluI GCNGC 2 cut(s) 6, 9
HaeIII GGCC 2 cut(s) 726, 762
HapII CCGG 3 cut(s) 215, 933, 963
HgaI GACGC 1 cut(s) 58
Hin1II CATG 6 cut(s) 258, 293, 325, 389, 706, 725
HincII GTYRAC 4 cut(s) 187, 518, 799, 826
HindII GTYRAC 4 cut(s) 187, 518, 799, 826
HindIII AAGCTT 1 cut(s) 77
HinfI GANTC 3 cut(s) 86, 146, 167
HpaI GTTAAC 1 cut(s) 826
HpaII CCGG 3 cut(s) 215, 933, 963
HphI GGTGA 4 cut(s) 292, 724, 830, 913
Hpy166II GTNNAC 6 cut(s) 187, 475, 518, 644, 799, 826
Hpy188I TCNGA 5 cut(s) 85, 351, 529, 637, 718
Hpy188III TCNNGA 5 cut(s) 73, 140, 215, 299, 944
Hpy8I GTNNAC 6 cut(s) 187, 475, 518, 644, 799, 826
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 4 cut(s) 120, 632, 737, 773
HpyCH4III ACNGT 2 cut(s) 33, 925
HpyCH4V TGCA 5 cut(s) 8, 537, 659, 695, 772
HpyF3I CTNAG 6 cut(s) 300, 350, 480, 584, 589, 610
Hsp92II CATG 6 cut(s) 258, 293, 325, 389, 706, 725
Kpn2I TCCGGA 1 cut(s) 214
Ksp22I TGATCA 2 cut(s) 282, 718
KspAI GTTAAC 1 cut(s) 826
Kzo9I GATC 7 cut(s) 16, 282, 346, 648, 718, 835, 846
LmnI GCTCC 3 cut(s) 217, 304, 870
Lsp1109I GCAGC 1 cut(s) 20
LweI GCATC 1 cut(s) 975
MaeI CTAG 1 cut(s) 944
MaeIII GTNAC 4 cut(s) 25, 97, 553, 955
MalI GATC 7 cut(s) 18, 284, 348, 650, 720, 837, 848
MboI GATC 7 cut(s) 16, 282, 346, 648, 718, 835, 846
MboII GAAGA 3 cut(s) 188, 611, 643
MflI RGATCY 3 cut(s) 16, 648, 846
MluCI AATT 1 cut(s) 753
MlyI GAGTC 2 cut(s) 95, 155
MmeI TCCRAC 1 cut(s) 953
MroI TCCGGA 1 cut(s) 214
MseI TTAA 5 cut(s) 132, 335, 623, 756, 825
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 3 cut(s) 215, 933, 963
MspR9I CCNGG 1 cut(s) 278
MvaI CCWGG 1 cut(s) 278
NdeI CATATG 1 cut(s) 1004
NdeII GATC 7 cut(s) 16, 282, 346, 648, 718, 835, 846
NlaIII CATG 6 cut(s) 258, 293, 325, 389, 706, 725
NlaIV GGNNCC 3 cut(s) 18, 396, 412
NmuCI GTSAC 2 cut(s) 97, 955
NspI RCATGY 3 cut(s) 258, 389, 706
PaeI GCATGC 2 cut(s) 258, 706
PfeI GAWTC 1 cut(s) 167
PinAI ACCGGT 1 cut(s) 932
PkrI GCNGC 2 cut(s) 7, 10
PleI GAGTC 2 cut(s) 94, 154
PpsI GAGTC 2 cut(s) 94, 154
PshAI GACNNNNGTC 1 cut(s) 68
PshBI ATTAAT 1 cut(s) 756
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 3 cut(s) 18, 396, 412
PspPI GGNCC 3 cut(s) 225, 394, 935
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 3 cut(s) 16, 648, 846
RsaI GTAC 4 cut(s) 35, 211, 866, 907
RsaNI GTAC 4 cut(s) 34, 210, 865, 906
SaqAI TTAA 5 cut(s) 132, 335, 623, 756, 825
SatI GCNGC 2 cut(s) 6, 9
Sau3AI GATC 7 cut(s) 16, 282, 346, 648, 718, 835, 846
Sau96I GGNCC 3 cut(s) 225, 394, 935
ScaI AGTACT 1 cut(s) 35
SchI GAGTC 2 cut(s) 95, 155
ScrFI CCNGG 1 cut(s) 278
SfaNI GCATC 1 cut(s) 975
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 3 cut(s) 225, 394, 935
SmlI CTYRAG 3 cut(s) 138, 461, 842
SmoI CTYRAG 3 cut(s) 138, 461, 842
SphI GCATGC 2 cut(s) 258, 706
Sse9I AATT 1 cut(s) 753
SsiI CCGC 3 cut(s) 53, 317, 504
SspMI CTAG 1 cut(s) 944
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 2 cut(s) 33, 925
TaqI TCGA 2 cut(s) 113, 883
TasI AATT 1 cut(s) 753
TatI WGTACW 2 cut(s) 33, 864
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 5 cut(s) 132, 335, 623, 756, 825
Tru9I TTAA 5 cut(s) 132, 335, 623, 756, 825
TscAI CASTG 3 cut(s) 588, 702, 835
TseFI GTSAC 2 cut(s) 97, 955
TseI GCWGC 2 cut(s) 5, 8
Tsp45I GTSAC 2 cut(s) 97, 955
TspDTI ATGAA 3 cut(s) 326, 513, 643
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 3 cut(s) 588, 702, 835
VpaK11BI GGWCC 3 cut(s) 225, 394, 935
VspI ATTAAT 1 cut(s) 756
XbaI TCTAGA 1 cut(s) 943
XceI RCATGY 3 cut(s) 258, 389, 706
XmiI GTMKAC 1 cut(s) 474
XspI CTAG 1 cut(s) 944
ZrmI AGTACT 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.