RLG00000013727

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0017372
Physical Location & Seq
Reverse (-)
36982371 .. 36984144
1774 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013727

Sequence Viewer

Length: 1092 bp
ATGGCTGCGGCAACGGATCCAGCAGTTACACAGGACTCACATACTGCTCTACCGCCAATACTGACCAGCGTCAAGAAGCTTTCAGAGTCATCACAAGTCACTTCTATTCCTTCGACTTATACATACCCCGTTAATCCTCAAGATGAGTCAGTTTTGATAGATTACGATTCAAAAATCCCCATCGTTGACTTCTCTCTTCTCACATCTGGTACTCCGGAGCAGAGGTCCAAAGCCATCAGCGACCTTGGTAAAGCATGCGAGGACTGGGGCTTTTTCCTGGTGATCAATCATAGTGTTCCTGAGGAGCTGATGAAAGCGGTCATGGATGGAAGTTTAAGATTTTTTAATCTGAGTGAGGAGGAGAAGAACGAGTATCAAGGGAAGCATGTATTGGACCCGATAAGGTGTGGCACCAGCTTCAATGCTTCGGTGGATAAAGTCTTGTTTTGGAGAGATTTTCTCAAGGTTTTTGTATACCCTGAGTTTCACTTCCCTGATAAACCCGCTGGCTTCAGTCAACTTTCATCCGAGTATTGCAAGAGAACAAGAGAAGTTACAAGGGAACTACTAAAAGGAATATCACTGAGCTTAGGATTGGAAGCTGACTACTTAGATACGGCCTTAAATGTGAAATCCGAAGGTTTACAGGTCTTCATTGCAAACTTGTATCCTCCTTGCCCACAGCCAGAGCTTGCACTGGGTATGCCCCCTCATTCTGATCATGGCCTCCTCACCCTCCTCGTTCAAAATGGAATTAATGGCCTTCAAGTGCAACACAATGGTAAGTGGGTCAATGTCAACGCCATACCCAACTCCTTTCTTGTTAACACTGGCGATCACCTTGAGATCTTTAGCAATGGGAAGTACAGGAGCAACATACATCGAGCAGCGCTAAACAACAAAGGTACAAGAATATCTATCGCCGTTCCAACTGGTCCATCTCTAGATGTAGTTGTGACTCCAGCATCAAAGTTGATAGAAAATGGAAATCATCCACCAGCATATGTTGGGATGAAGTATAAGGATTATCTAGAACTTCAGCAAAGCAGCATGCTTGATGGGAAAGCGGTCTTAGATCGTGTGCGTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

364

Amino Acids

40.13

Weight (kDa)

5.7

Isoelectric Point (pI)

35.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 59 - 161 2.8e-24 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 216 - 310 3.7e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017372)

Species Orthologous Gene IDs
malus_domestica MD10G1053500.v1.1
prunus_persica Prupe.8G066900_v2.0.a1
pyrus_communis pycom10g04050
rosa_chinensis RchiOBHm_Chr6g0271361
rosa_laevigata RLG00000013727
rosa_multiflora Rmu_sc0004866.1_g000028
rosa_roxburghii Rroxscaffold_7G00197070
rosa_rugosa Rorug06G0060500
rosa_samantha Rh6AG179600 Rh6BG182600 Rh6CG179500 Rh6DG171200
rosa_wichuraiana Rw6G015440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 410
AccI GTMKAC 1 cut(s) 474
AccIII TCCGGA 1 cut(s) 214
AciI CCGC 5 cut(s) 8, 53, 317, 504, 1067
AclWI GGATC 2 cut(s) 11, 24
AcuI CTGAAG 2 cut(s) 496, 1022
AfaI GTAC 3 cut(s) 211, 866, 907
AfeI AGCGCT 1 cut(s) 891
AgsI TTSAA 4 cut(s) 171, 421, 746, 767
AjnI CCWGG 1 cut(s) 276
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
AluBI AGCT 6 cut(s) 79, 307, 417, 588, 602, 691
AluI AGCT 6 cut(s) 79, 307, 417, 588, 602, 691
AlwI GGATC 2 cut(s) 11, 24
Aor13HI TCCGGA 1 cut(s) 214
Aor51HI AGCGCT 1 cut(s) 891
AoxI GGCC 3 cut(s) 618, 724, 760
ApeKI GCWGC 3 cut(s) 5, 887, 1047
AseI ATTAAT 1 cut(s) 756
AspLEI GCGC 1 cut(s) 892
AspS9I GGNCC 3 cut(s) 225, 394, 935
AsuHPI GGTGA 3 cut(s) 292, 724, 830
AvaII GGWCC 3 cut(s) 225, 394, 935
AxyI CCTNAGG 1 cut(s) 300
BamHI GGATCC 1 cut(s) 16
BanI GGYRCC 1 cut(s) 410
BbsI GAAGAC 1 cut(s) 643
BbvI GCAGC 2 cut(s) 899, 1059
BccI CCATC 5 cut(s) 188, 242, 320, 946, 1052
BceAI ACGGC 2 cut(s) 633, 908
BciT130I CCWGG 1 cut(s) 278
BciVI GTATCC 1 cut(s) 678
BclI TGATCA 2 cut(s) 282, 718
BfaI CTAG 2 cut(s) 944, 1031
BfoI RGCGCY 1 cut(s) 893
BfuI GTATCC 1 cut(s) 678
BglII AGATCT 1 cut(s) 846
BisI GCNGC 4 cut(s) 6, 9, 888, 1048
BlsI GCNGC 4 cut(s) 7, 10, 889, 1049
Bme1390I CCNGG 1 cut(s) 278
Bme18I GGWCC 3 cut(s) 225, 394, 935
BmgT120I GGNCC 3 cut(s) 225, 394, 935
BmiI GGNNCC 3 cut(s) 18, 396, 412
BmrFI CCNGG 1 cut(s) 278
BmrI ACTGGG 2 cut(s) 274, 707
BmsI GCATC 1 cut(s) 974
BmuI ACTGGG 2 cut(s) 274, 707
BoxI GACNNNNGTC 1 cut(s) 68
BpiI GAAGAC 1 cut(s) 643
BplI GAGNNNNNCTC 2 cut(s) 444, 476
BpmI CTGGAG 1 cut(s) 945
Bpu10I CCTNAGC 1 cut(s) 589
BpuEI CTTGAG 3 cut(s) 123, 446, 863
BsaJI CCNNGG 1 cut(s) 244
BsaWI WCCGGW 1 cut(s) 214
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bse1I ACTGG 4 cut(s) 269, 702, 835, 937
Bse21I CCTNAGG 1 cut(s) 300
Bse3DI GCAATG 2 cut(s) 654, 862
BseAI TCCGGA 1 cut(s) 214
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 1 cut(s) 244
BseGI GGATG 4 cut(s) 331, 524, 991, 1017
BseMI GCAATG 2 cut(s) 654, 862
BseMII CTCAG 4 cut(s) 291, 341, 471, 575
BseNI ACTGG 4 cut(s) 269, 702, 835, 937
BseRI GAGGAG 5 cut(s) 317, 371, 374, 719, 728
BseXI GCAGC 2 cut(s) 899, 1059
BshFI GGCC 3 cut(s) 620, 726, 762
BshNI GGYRCC 1 cut(s) 410
BsiSI CCGG 1 cut(s) 215
BsnI GGCC 3 cut(s) 620, 726, 762
Bsp13I TCCGGA 1 cut(s) 214
Bsp143I GATC 6 cut(s) 16, 282, 718, 835, 846, 1075
BspACI CCGC 5 cut(s) 8, 53, 317, 504, 1067
BspANI GGCC 3 cut(s) 620, 726, 762
BspCNI CTCAG 4 cut(s) 292, 342, 472, 576
BspEI TCCGGA 1 cut(s) 214
BspLI GGNNCC 3 cut(s) 18, 396, 412
BspPI GGATC 2 cut(s) 11, 24
BspT107I GGYRCC 1 cut(s) 410
BsrDI GCAATG 2 cut(s) 654, 862
BsrI ACTGG 4 cut(s) 269, 702, 835, 937
BssECI CCNNGG 1 cut(s) 244
BssMI GATC 6 cut(s) 16, 282, 718, 835, 846, 1075
BssNAI GTATAC 1 cut(s) 475
BssT1I CCWWGG 1 cut(s) 244
Bst1107I GTATAC 1 cut(s) 475
Bst2UI CCWGG 1 cut(s) 278
Bst6I CTCTTC 1 cut(s) 201
BstC8I GCNNGC 4 cut(s) 256, 508, 693, 1052
BstDEI CTNAG 7 cut(s) 300, 350, 480, 584, 589, 610, 1072
BstF5I GGATG 4 cut(s) 331, 524, 991, 1017
BstH2I RGCGCY 1 cut(s) 893
BstHHI GCGC 1 cut(s) 892
BstKTI GATC 6 cut(s) 19, 285, 721, 838, 849, 1078
BstMBI GATC 6 cut(s) 16, 282, 718, 835, 846, 1075
BstNI CCWGG 1 cut(s) 278
BstNSI RCATGY 3 cut(s) 258, 389, 1054
BstPAI GACNNNNGTC 1 cut(s) 68
BstSCI CCNGG 1 cut(s) 276
BstV1I GCAGC 2 cut(s) 899, 1059
BstV2I GAAGAC 1 cut(s) 643
BstX2I RGATCY 2 cut(s) 16, 846
BstYI RGATCY 2 cut(s) 16, 846
BstZ17I GTATAC 1 cut(s) 475
Bsu36I CCTNAGG 1 cut(s) 300
BsuI GTATCC 1 cut(s) 678
BsuRI GGCC 3 cut(s) 620, 726, 762
BtsCI GGATG 4 cut(s) 331, 524, 991, 1017
BtsIMutI CAGTG 3 cut(s) 581, 695, 828
Cac8I GCNNGC 4 cut(s) 256, 508, 693, 1052
CfoI GCGC 1 cut(s) 892
Cfr13I GGNCC 3 cut(s) 225, 394, 935
CseI GACGC 1 cut(s) 58
Csp6I GTAC 3 cut(s) 210, 865, 906
CviAII CATG 5 cut(s) 255, 322, 386, 722, 1051
CviQI GTAC 3 cut(s) 210, 865, 906
DdeI CTNAG 7 cut(s) 300, 350, 480, 584, 589, 610, 1072
DpnI GATC 6 cut(s) 18, 284, 720, 837, 848, 1077
DpnII GATC 6 cut(s) 16, 282, 718, 835, 846, 1075
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco130I CCWWGG 1 cut(s) 244
Eco47I GGWCC 3 cut(s) 225, 394, 935
Eco47III AGCGCT 1 cut(s) 891
Eco57I CTGAAG 2 cut(s) 496, 1022
Eco81I CCTNAGG 1 cut(s) 300
EcoRII CCWGG 1 cut(s) 276
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 5 cut(s) 258, 325, 389, 725, 1054
FatI CATG 5 cut(s) 254, 321, 385, 721, 1050
FauI CCCGC 1 cut(s) 511
FauNDI CATATG 1 cut(s) 1003
FbaI TGATCA 2 cut(s) 282, 718
FblI GTMKAC 1 cut(s) 474
Fnu4HI GCNGC 4 cut(s) 6, 9, 888, 1048
FokI GGATG 4 cut(s) 338, 511, 978, 1024
Fsp4HI GCNGC 4 cut(s) 6, 9, 888, 1048
FspBI CTAG 2 cut(s) 944, 1031
GlaI GCGC 1 cut(s) 891
GluI GCNGC 4 cut(s) 6, 9, 888, 1048
GsuI CTGGAG 1 cut(s) 945
HaeII RGCGCY 1 cut(s) 893
HaeIII GGCC 3 cut(s) 620, 726, 762
HapII CCGG 1 cut(s) 215
HgaI GACGC 1 cut(s) 58
HhaI GCGC 1 cut(s) 892
Hin1II CATG 5 cut(s) 258, 325, 389, 725, 1054
Hin6I GCGC 1 cut(s) 890
HinP1I GCGC 1 cut(s) 890
HincII GTYRAC 4 cut(s) 187, 518, 799, 826
HindII GTYRAC 4 cut(s) 187, 518, 799, 826
HindIII AAGCTT 1 cut(s) 77
HinfI GANTC 5 cut(s) 35, 86, 146, 167, 958
HpaI GTTAAC 1 cut(s) 826
HpaII CCGG 1 cut(s) 215
HphI GGTGA 3 cut(s) 292, 724, 830
Hpy166II GTNNAC 6 cut(s) 187, 475, 518, 644, 799, 826
Hpy188I TCNGA 5 cut(s) 85, 351, 529, 637, 718
Hpy188III TCNNGA 6 cut(s) 73, 140, 215, 299, 944, 1031
Hpy8I GTNNAC 6 cut(s) 187, 475, 518, 644, 799, 826
HpyAV CCTTC 3 cut(s) 120, 632, 773
HpyCH4V TGCA 4 cut(s) 537, 659, 695, 772
HpyF3I CTNAG 7 cut(s) 300, 350, 480, 584, 589, 610, 1072
Hsp92II CATG 5 cut(s) 258, 325, 389, 725, 1054
HspAI GCGC 1 cut(s) 890
Kpn2I TCCGGA 1 cut(s) 214
Ksp22I TGATCA 2 cut(s) 282, 718
KspAI GTTAAC 1 cut(s) 826
Kzo9I GATC 6 cut(s) 16, 282, 718, 835, 846, 1075
LmnI GCTCC 3 cut(s) 217, 304, 870
Lsp1109I GCAGC 2 cut(s) 899, 1059
LweI GCATC 1 cut(s) 974
MaeI CTAG 2 cut(s) 944, 1031
MaeIII GTNAC 4 cut(s) 25, 97, 553, 955
MalI GATC 6 cut(s) 18, 284, 720, 837, 848, 1077
MboI GATC 6 cut(s) 16, 282, 718, 835, 846, 1075
MboII GAAGA 3 cut(s) 188, 376, 643
MflI RGATCY 2 cut(s) 16, 846
MluCI AATT 1 cut(s) 753
MlyI GAGTC 4 cut(s) 29, 95, 155, 952
MmeI TCCRAC 1 cut(s) 953
MroI TCCGGA 1 cut(s) 214
MseI TTAA 7 cut(s) 132, 335, 345, 623, 756, 825, 1090
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 1 cut(s) 215
MspR9I CCNGG 1 cut(s) 278
MvaI CCWGG 1 cut(s) 278
NdeI CATATG 1 cut(s) 1003
NdeII GATC 6 cut(s) 16, 282, 718, 835, 846, 1075
NlaIII CATG 5 cut(s) 258, 325, 389, 725, 1054
NlaIV GGNNCC 3 cut(s) 18, 396, 412
NmuCI GTSAC 2 cut(s) 97, 955
NspI RCATGY 3 cut(s) 258, 389, 1054
PaeI GCATGC 2 cut(s) 258, 1054
PfeI GAWTC 1 cut(s) 167
PkrI GCNGC 4 cut(s) 7, 10, 889, 1049
PleI GAGTC 4 cut(s) 29, 94, 154, 952
PpsI GAGTC 4 cut(s) 29, 94, 154, 952
PshAI GACNNNNGTC 1 cut(s) 68
PshBI ATTAAT 1 cut(s) 756
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 3 cut(s) 18, 396, 412
PspPI GGNCC 3 cut(s) 225, 394, 935
PsuI RGATCY 2 cut(s) 16, 846
RsaI GTAC 3 cut(s) 211, 866, 907
RsaNI GTAC 3 cut(s) 210, 865, 906
SaqAI TTAA 7 cut(s) 132, 335, 345, 623, 756, 825, 1090
SatI GCNGC 4 cut(s) 6, 9, 888, 1048
Sau3AI GATC 6 cut(s) 16, 282, 718, 835, 846, 1075
Sau96I GGNCC 3 cut(s) 225, 394, 935
SchI GAGTC 4 cut(s) 29, 95, 155, 952
ScrFI CCNGG 1 cut(s) 278
SfaNI GCATC 1 cut(s) 974
SinI GGWCC 3 cut(s) 225, 394, 935
SmlI CTYRAG 3 cut(s) 138, 461, 842
SmoI CTYRAG 3 cut(s) 138, 461, 842
SphI GCATGC 2 cut(s) 258, 1054
Sse9I AATT 1 cut(s) 753
SsiI CCGC 5 cut(s) 8, 53, 317, 504, 1067
SspMI CTAG 2 cut(s) 944, 1031
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 1 cut(s) 244
TaqI TCGA 2 cut(s) 113, 883
TasI AATT 1 cut(s) 753
TatI WGTACW 1 cut(s) 864
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 7 cut(s) 132, 335, 345, 623, 756, 825, 1090
Tru9I TTAA 7 cut(s) 132, 335, 345, 623, 756, 825, 1090
TscAI CASTG 3 cut(s) 588, 702, 835
TseFI GTSAC 2 cut(s) 97, 955
TseI GCWGC 3 cut(s) 5, 887, 1047
Tsp45I GTSAC 2 cut(s) 97, 955
TspDTI ATGAA 4 cut(s) 326, 513, 643, 1028
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 3 cut(s) 588, 702, 835
VpaK11BI GGWCC 3 cut(s) 225, 394, 935
VspI ATTAAT 1 cut(s) 756
XbaI TCTAGA 2 cut(s) 943, 1030
XceI RCATGY 3 cut(s) 258, 389, 1054
XmiI GTMKAC 1 cut(s) 474
XspI CTAG 2 cut(s) 944, 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.