Rmu_sc0004866.1_g000028

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004866.1
OG0017372
Physical Location & Seq
Reverse (-)
162599 .. 164806
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004866.1_g000028.1.cds

Sequence Viewer

Length: 1311 bp
atggggttgatctcggggattctgatggggatgttgttcggaattgagttgatggccggttggcgtcacctgatggcttaccgaagtaccaagctagtcgctaaggtagttgatataaaactccttggaatcaaagatgaaagaagaagagatgatgacaatgatatcacggctaccggggaacaggctaagcccttcccacctacatttgctcttatcaaattgacaccaacggatccagcagttacacagtactcacatactgctctaccgccaatactgacaagcgtcaagaagctttcagagtcatcacaagtcacttctattccttcgacttatacataccccgttaatcctcaagatgagtcagttttgacagattacgattcaaaaatccccatcgttgacttctctcttctcacatctggtactccggagcagaggtccaaagccatcagcgaccttggtaaagcatgcgaggactggggctttttcctggtgatcaatcatggtgttcctgaggagctgatgaaagcggtcatggatggaagtttaagatttttttatctgagtgaggaggagaagcacgagtatcaagggaagcatgtattggacccgataaggtgtggcaccagcttcaatgcttcggtggataaagtcttgttttggagagattttctcaaggtttttgtataccctgaatttcacttccctgataaacccgctggcttcagtcaactttcatccgagtattgcaagagaacaagagaagttacaagggaactactaaaaggaatatcactgagcttaggattggaggctgactacttagatacggccttaaatgtgaaatccgaaggtttacaggtcttcattgcaaacttgtatcctccttgcccacagccagagcttgcactgggcatgccccctcattctgatcatggcctcctcaccctcctcattcaaaatggaattaatggccttcaagtgcaacacaatggtaagtgggtcaatgtcaacgccatacccaactcctttcttgttaacactggcgatcaccttgagatctttagcaatgggaagtacaggagcaacatacatcgagcagcgctaaacaacaaaggtacaagaatatctatcgccgttccaactggtccatctctagatgtagttgtgactccggcatcaaagttggtagaaaatggaaatcatccaccagcatatgttgggatgaagtataaggattatctagaacttcagcaaagcagcatgcttaatgggaaagcggtcttagatcgtgtgcgtatttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

436

Amino Acids

48.43

Weight (kDa)

6.32

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017372)

Species Orthologous Gene IDs
malus_domestica MD10G1053500.v1.1
prunus_persica Prupe.8G066900_v2.0.a1
pyrus_communis pycom10g04050
rosa_chinensis RchiOBHm_Chr6g0271361
rosa_laevigata RLG00000013727
rosa_multiflora Rmu_sc0004866.1_g000028
rosa_roxburghii Rroxscaffold_7G00197070
rosa_rugosa Rorug06G0060500
rosa_samantha Rh6AG179600 Rh6BG182600 Rh6CG179500 Rh6DG171200
rosa_wichuraiana Rw6G015440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 629
AccI GTMKAC 1 cut(s) 693
AccIII TCCGGA 1 cut(s) 433
AciI CCGC 4 cut(s) 272, 536, 723, 1286
AclWI GGATC 2 cut(s) 230, 243
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 701
AcuI CTGAAG 2 cut(s) 715, 1241
AcyI GRCGYC 1 cut(s) 64
AfaI GTAC 5 cut(s) 88, 254, 430, 1085, 1126
AfeI AGCGCT 1 cut(s) 1110
AgsI TTSAA 4 cut(s) 390, 640, 965, 986
AjnI CCWGG 1 cut(s) 495
AjuI GAANNNNNNNTTGG 2 cut(s) 593, 625
AluBI AGCT 6 cut(s) 94, 298, 526, 636, 807, 910
AluI AGCT 6 cut(s) 94, 298, 526, 636, 807, 910
AlwI GGATC 2 cut(s) 230, 243
Ama87I CYCGRG 1 cut(s) 13
Aor13HI TCCGGA 1 cut(s) 433
Aor51HI AGCGCT 1 cut(s) 1110
AoxI GGCC 4 cut(s) 54, 837, 943, 979
ApeKI GCWGC 2 cut(s) 1106, 1266
ApoI RAATTY 1 cut(s) 701
AseI ATTAAT 1 cut(s) 975
AspLEI GCGC 1 cut(s) 1111
AspS9I GGNCC 3 cut(s) 444, 613, 1154
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 4 cut(s) 59, 511, 943, 1049
AvaI CYCGRG 1 cut(s) 13
AvaII GGWCC 3 cut(s) 444, 613, 1154
AxyI CCTNAGG 1 cut(s) 519
BaeI ACNNNNGTAYC 2 cut(s) 70, 103
BamHI GGATCC 1 cut(s) 235
BanI GGYRCC 1 cut(s) 629
BauI CACGAG 1 cut(s) 587
BbsI GAAGAC 1 cut(s) 862
BbvI GCAGC 2 cut(s) 1118, 1278
BccI CCATC 7 cut(s) 19, 46, 67, 407, 461, 539, 1165
BceAI ACGGC 3 cut(s) 186, 852, 1127
BciT130I CCWGG 1 cut(s) 497
BciVI GTATCC 1 cut(s) 897
BclI TGATCA 2 cut(s) 501, 937
BcnI CCSGG 1 cut(s) 178
BfaI CTAG 3 cut(s) 95, 1163, 1250
BfoI RGCGCY 1 cut(s) 1112
BfuI GTATCC 1 cut(s) 897
BglII AGATCT 1 cut(s) 1065
BisI GCNGC 2 cut(s) 1107, 1267
BlpI GCTNAGC 1 cut(s) 189
BlsI GCNGC 2 cut(s) 1108, 1268
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 2 cut(s) 178, 497
Bme18I GGWCC 3 cut(s) 444, 613, 1154
BmeT110I CYCGRG 1 cut(s) 13
BmgT120I GGNCC 3 cut(s) 444, 613, 1154
BmiI GGNNCC 3 cut(s) 237, 615, 631
BmrFI CCNGG 2 cut(s) 178, 497
BmrI ACTGGG 2 cut(s) 493, 926
BmsI GCATC 1 cut(s) 1193
BmuI ACTGGG 2 cut(s) 493, 926
BoxI GACNNNNGTC 1 cut(s) 287
BpiI GAAGAC 1 cut(s) 862
BplI GAGNNNNNCTC 2 cut(s) 663, 695
Bpu10I CCTNAGC 2 cut(s) 102, 808
Bpu1102I GCTNAGC 1 cut(s) 189
BpuEI CTTGAG 3 cut(s) 342, 665, 1082
BpuMI CCSGG 1 cut(s) 178
BsaHI GRCGYC 1 cut(s) 64
BsaJI CCNNGG 3 cut(s) 124, 177, 463
BsaWI WCCGGW 1 cut(s) 433
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 4 cut(s) 488, 921, 1054, 1156
Bse21I CCTNAGG 1 cut(s) 519
Bse3DI GCAATG 2 cut(s) 873, 1081
BseAI TCCGGA 1 cut(s) 433
BseBI CCWGG 1 cut(s) 497
BseDI CCNNGG 3 cut(s) 124, 177, 463
BseGI GGATG 5 cut(s) 36, 550, 743, 1210, 1236
BseMI GCAATG 2 cut(s) 873, 1081
BseMII CTCAG 3 cut(s) 510, 560, 794
BseNI ACTGG 4 cut(s) 488, 921, 1054, 1156
BseRI GAGGAG 5 cut(s) 536, 590, 593, 938, 947
BseXI GCAGC 2 cut(s) 1118, 1278
BshFI GGCC 4 cut(s) 56, 839, 945, 981
BshNI GGYRCC 1 cut(s) 629
BsiHKCI CYCGRG 1 cut(s) 13
BsiSI CCGG 4 cut(s) 57, 177, 434, 1181
BsnI GGCC 4 cut(s) 56, 839, 945, 981
BsoBI CYCGRG 1 cut(s) 13
Bsp13I TCCGGA 1 cut(s) 433
Bsp143I GATC 7 cut(s) 9, 235, 501, 937, 1054, 1065, 1294
Bsp1720I GCTNAGC 1 cut(s) 189
BspACI CCGC 4 cut(s) 272, 536, 723, 1286
BspANI GGCC 4 cut(s) 56, 839, 945, 981
BspCNI CTCAG 3 cut(s) 511, 561, 795
BspEI TCCGGA 1 cut(s) 433
BspLI GGNNCC 3 cut(s) 237, 615, 631
BspPI GGATC 2 cut(s) 230, 243
BspT107I GGYRCC 1 cut(s) 629
BsrDI GCAATG 2 cut(s) 873, 1081
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 4 cut(s) 488, 921, 1054, 1156
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 3 cut(s) 124, 177, 463
BssMI GATC 7 cut(s) 9, 235, 501, 937, 1054, 1065, 1294
BssNAI GTATAC 1 cut(s) 694
BssNI GRCGYC 1 cut(s) 64
BssSI CACGAG 1 cut(s) 587
BssT1I CCWWGG 2 cut(s) 124, 463
Bst1107I GTATAC 1 cut(s) 694
Bst2BI CACGAG 1 cut(s) 587
Bst2UI CCWGG 1 cut(s) 497
Bst4CI ACNGT 1 cut(s) 252
Bst6I CTCTTC 2 cut(s) 142, 420
BstACI GRCGYC 1 cut(s) 64
BstC8I GCNNGC 5 cut(s) 475, 727, 912, 923, 1271
BstDEI CTNAG 8 cut(s) 102, 189, 519, 569, 803, 808, 829, 1291
BstF5I GGATG 5 cut(s) 36, 550, 743, 1210, 1236
BstH2I RGCGCY 1 cut(s) 1112
BstHHI GCGC 1 cut(s) 1111
BstKTI GATC 7 cut(s) 12, 238, 504, 940, 1057, 1068, 1297
BstMBI GATC 7 cut(s) 9, 235, 501, 937, 1054, 1065, 1294
BstNI CCWGG 1 cut(s) 497
BstNSI RCATGY 4 cut(s) 477, 608, 925, 1273
BstPAI GACNNNNGTC 1 cut(s) 287
BstSCI CCNGG 2 cut(s) 176, 495
BstV1I GCAGC 2 cut(s) 1118, 1278
BstV2I GAAGAC 1 cut(s) 862
BstX2I RGATCY 2 cut(s) 235, 1065
BstYI RGATCY 2 cut(s) 235, 1065
BstZ17I GTATAC 1 cut(s) 694
Bsu36I CCTNAGG 1 cut(s) 519
BsuI GTATCC 1 cut(s) 897
BsuRI GGCC 4 cut(s) 56, 839, 945, 981
BtsCI GGATG 5 cut(s) 36, 550, 743, 1210, 1236
BtsIMutI CAGTG 3 cut(s) 800, 914, 1047
Cac8I GCNNGC 5 cut(s) 475, 727, 912, 923, 1271
CfoI GCGC 1 cut(s) 1111
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 3 cut(s) 444, 613, 1154
CseI GACGC 2 cut(s) 53, 277
Csp6I GTAC 5 cut(s) 87, 253, 429, 1084, 1125
CviAII CATG 7 cut(s) 474, 509, 541, 605, 922, 941, 1270
CviQI GTAC 5 cut(s) 87, 253, 429, 1084, 1125
DdeI CTNAG 8 cut(s) 102, 189, 519, 569, 803, 808, 829, 1291
DpnI GATC 7 cut(s) 11, 237, 503, 939, 1056, 1067, 1296
DpnII GATC 7 cut(s) 9, 235, 501, 937, 1054, 1065, 1294
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 2 cut(s) 142, 420
EarI CTCTTC 2 cut(s) 142, 420
Eco130I CCWWGG 2 cut(s) 124, 463
Eco32I GATATC 1 cut(s) 166
Eco47I GGWCC 3 cut(s) 444, 613, 1154
Eco47III AGCGCT 1 cut(s) 1110
Eco57I CTGAAG 2 cut(s) 715, 1241
Eco81I CCTNAGG 1 cut(s) 519
Eco88I CYCGRG 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 495
EcoRV GATATC 1 cut(s) 166
EcoT14I CCWWGG 2 cut(s) 124, 463
ErhI CCWWGG 2 cut(s) 124, 463
FaeI CATG 7 cut(s) 477, 512, 544, 608, 925, 944, 1273
FatI CATG 7 cut(s) 473, 508, 540, 604, 921, 940, 1269
FauI CCCGC 1 cut(s) 730
FauNDI CATATG 1 cut(s) 1222
FbaI TGATCA 2 cut(s) 501, 937
FblI GTMKAC 1 cut(s) 693
Fnu4HI GCNGC 2 cut(s) 1107, 1267
FokI GGATG 5 cut(s) 43, 557, 730, 1197, 1243
Fsp4HI GCNGC 2 cut(s) 1107, 1267
FspBI CTAG 3 cut(s) 95, 1163, 1250
GlaI GCGC 1 cut(s) 1110
GluI GCNGC 2 cut(s) 1107, 1267
HaeII RGCGCY 1 cut(s) 1112
HaeIII GGCC 4 cut(s) 56, 839, 945, 981
HapII CCGG 4 cut(s) 57, 177, 434, 1181
HgaI GACGC 2 cut(s) 53, 277
HhaI GCGC 1 cut(s) 1111
Hin1I GRCGYC 1 cut(s) 64
Hin1II CATG 7 cut(s) 477, 512, 544, 608, 925, 944, 1273
Hin6I GCGC 1 cut(s) 1109
HinP1I GCGC 1 cut(s) 1109
HincII GTYRAC 4 cut(s) 406, 737, 1018, 1045
HindII GTYRAC 4 cut(s) 406, 737, 1018, 1045
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 6 cut(s) 19, 129, 305, 365, 386, 1177
HpaI GTTAAC 1 cut(s) 1045
HpaII CCGG 4 cut(s) 57, 177, 434, 1181
HphI GGTGA 4 cut(s) 59, 511, 943, 1049
Hpy166II GTNNAC 6 cut(s) 406, 694, 737, 863, 1018, 1045
Hpy188I TCNGA 7 cut(s) 24, 41, 304, 570, 748, 856, 937
Hpy188III TCNNGA 6 cut(s) 292, 359, 434, 518, 1163, 1250
Hpy8I GTNNAC 6 cut(s) 406, 694, 737, 863, 1018, 1045
HpyAV CCTTC 4 cut(s) 205, 339, 851, 992
HpyCH4III ACNGT 1 cut(s) 252
HpyCH4V TGCA 4 cut(s) 756, 878, 914, 991
HpyF3I CTNAG 8 cut(s) 102, 189, 519, 569, 803, 808, 829, 1291
Hsp92I GRCGYC 1 cut(s) 64
Hsp92II CATG 7 cut(s) 477, 512, 544, 608, 925, 944, 1273
HspAI GCGC 1 cut(s) 1109
Kpn2I TCCGGA 1 cut(s) 433
Ksp22I TGATCA 2 cut(s) 501, 937
KspAI GTTAAC 1 cut(s) 1045
Kzo9I GATC 7 cut(s) 9, 235, 501, 937, 1054, 1065, 1294
LmnI GCTCC 3 cut(s) 436, 523, 1089
Lsp1109I GCAGC 2 cut(s) 1118, 1278
LweI GCATC 1 cut(s) 1193
MaeI CTAG 3 cut(s) 95, 1163, 1250
MaeIII GTNAC 5 cut(s) 65, 244, 316, 772, 1174
MalI GATC 7 cut(s) 11, 237, 503, 939, 1056, 1067, 1296
MboI GATC 7 cut(s) 9, 235, 501, 937, 1054, 1065, 1294
MboII GAAGA 4 cut(s) 156, 159, 407, 862
MflI RGATCY 2 cut(s) 235, 1065
MluCI AATT 4 cut(s) 42, 221, 701, 972
MlyI GAGTC 3 cut(s) 314, 374, 1171
MmeI TCCRAC 1 cut(s) 1172
MroI TCCGGA 1 cut(s) 433
MseI TTAA 7 cut(s) 351, 554, 842, 975, 1044, 1275, 1309
MspA1I CMGCKG 1 cut(s) 725
MspI CCGG 4 cut(s) 57, 177, 434, 1181
MspR9I CCNGG 2 cut(s) 178, 497
MvaI CCWGG 1 cut(s) 497
NciI CCSGG 1 cut(s) 178
NdeI CATATG 1 cut(s) 1222
NdeII GATC 7 cut(s) 9, 235, 501, 937, 1054, 1065, 1294
NlaIII CATG 7 cut(s) 477, 512, 544, 608, 925, 944, 1273
NlaIV GGNNCC 3 cut(s) 237, 615, 631
NmuCI GTSAC 3 cut(s) 65, 316, 1174
NspI RCATGY 4 cut(s) 477, 608, 925, 1273
PaeI GCATGC 3 cut(s) 477, 925, 1273
PfeI GAWTC 3 cut(s) 19, 129, 386
PkrI GCNGC 2 cut(s) 1108, 1268
PleI GAGTC 3 cut(s) 313, 373, 1171
PpsI GAGTC 3 cut(s) 313, 373, 1171
PshAI GACNNNNGTC 1 cut(s) 287
PshBI ATTAAT 1 cut(s) 975
Psp6I CCWGG 1 cut(s) 495
PspGI CCWGG 1 cut(s) 495
PspN4I GGNNCC 3 cut(s) 237, 615, 631
PspPI GGNCC 3 cut(s) 444, 613, 1154
PsuI RGATCY 2 cut(s) 235, 1065
RsaI GTAC 5 cut(s) 88, 254, 430, 1085, 1126
RsaNI GTAC 5 cut(s) 87, 253, 429, 1084, 1125
SaqAI TTAA 7 cut(s) 351, 554, 842, 975, 1044, 1275, 1309
SatI GCNGC 2 cut(s) 1107, 1267
Sau3AI GATC 7 cut(s) 9, 235, 501, 937, 1054, 1065, 1294
Sau96I GGNCC 3 cut(s) 444, 613, 1154
ScaI AGTACT 1 cut(s) 254
SchI GAGTC 3 cut(s) 314, 374, 1171
ScrFI CCNGG 2 cut(s) 178, 497
SfaNI GCATC 1 cut(s) 1193
SinI GGWCC 3 cut(s) 444, 613, 1154
SmlI CTYRAG 3 cut(s) 357, 680, 1061
SmoI CTYRAG 3 cut(s) 357, 680, 1061
SphI GCATGC 3 cut(s) 477, 925, 1273
Sse9I AATT 4 cut(s) 42, 221, 701, 972
SsiI CCGC 4 cut(s) 272, 536, 723, 1286
SspMI CTAG 3 cut(s) 95, 1163, 1250
StyD4I CCNGG 2 cut(s) 176, 495
StyI CCWWGG 2 cut(s) 124, 463
TaaI ACNGT 1 cut(s) 252
TaqI TCGA 2 cut(s) 332, 1102
TasI AATT 4 cut(s) 42, 221, 701, 972
TatI WGTACW 2 cut(s) 252, 1083
TfiI GAWTC 3 cut(s) 19, 129, 386
Tru1I TTAA 7 cut(s) 351, 554, 842, 975, 1044, 1275, 1309
Tru9I TTAA 7 cut(s) 351, 554, 842, 975, 1044, 1275, 1309
TscAI CASTG 3 cut(s) 807, 921, 1054
TseFI GTSAC 3 cut(s) 65, 316, 1174
TseI GCWGC 2 cut(s) 1106, 1266
Tsp45I GTSAC 3 cut(s) 65, 316, 1174
TspDTI ATGAA 5 cut(s) 153, 545, 732, 862, 1247
TspGWI ACGGA 1 cut(s) 248
TspRI CASTG 3 cut(s) 807, 921, 1054
VpaK11BI GGWCC 3 cut(s) 444, 613, 1154
VspI ATTAAT 1 cut(s) 975
XapI RAATTY 1 cut(s) 701
XbaI TCTAGA 2 cut(s) 1162, 1249
XceI RCATGY 4 cut(s) 477, 608, 925, 1273
XmiI GTMKAC 1 cut(s) 693
XspI CTAG 3 cut(s) 95, 1163, 1250
ZrmI AGTACT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.