MD10G1055800.v1.1
ERF Family

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin Fzo YdjA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006045
Physical Location & Seq
Forward (+)
7643761 .. 7648582
4822 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1055800.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGACTACATCAATACTTCACACACAAATTTTGTTGGTGGGAGTAAGGCTGTGGAGCTTGCATTGCAACAGGTGAAATCCTCTCGGATACCACTGCCCCTTTCAAGGCAAAAGGATGGTGTAGATTCTGATAAGGCACCAACATCTGAAAGAAGTTTGAAATCTAGAGCAATTCTTGCCAGACCAGTAAATGGAATTGTGCCTGACCAGGGTGCAAGACCTGTGGCAGACAATGAAAAAGTTGCATCTTCTGGGAATATATTAGGAGGTGCAACTGGTTCAAGTTGGGGGATTTCTTCAATTTTTGGTGGGAATGATAACAACCGCACACCTGCTAAAGAAAGCTTAATAAACAAGTCATATAATGAACCTATCCACAACATTGAACAAGCAGTCTCAATGATCCATTTAAGAGAGCCCCCAACTGTATTGAGGCCCACAGAGAGCCACTCAGAGCAGGAGACAATTGAAATTGCAGTCACAAAACTCCTATTAAGATCATACTATGACATTGTTAGGAAAAATATAGAGGATTCTGTACCAAAAGCAATAATGCACTTCCTGGTAAATCATACGAAGCGTGAGCTGCACAACGTCTTCATTAAGAAGCTTTACCGTGAGAACCTATTTGAAGAGATGCTGCAGGAGCCAGATGAAGTAGCAATGAAGAGAAAGCGCACCCGGGAAACCCTCCGAGTTCTTCAACAGGCTTTTCGGACATTGGATGAGCTGCCTTTGGAAGCCGACACAGTTGAAAAAGGTTACAGTTTGGGTGCTGATCCAACAGGGTTGCCAAAGATCCATGGATTGCCAACATCATCAATGTACAATACAAGTAGTTCACATGATTCCTACACAGCATCACCTAAGAATACGAAGTCTAGAAAGTCGTCTCACTCGGGGGAGCTTCACTCGCCATTTTATTCTAATGCGGATTCAAACGGAAGTGGACGCATGTACATGCCTGGTCTATATCCAACTGTTGATCAATAA

Protein Analysis

331

Amino Acids

36.61

Weight (kDa)

8.53

Isoelectric Point (pI)

60.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GED PF02212 153 - 243 6e-28 Dynamin GTPase effector domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 340
Acc36I ACCTGC 1 cut(s) 340
AccB1I GGYRCC 1 cut(s) 136
AccB7I CCANNNNNTGG 1 cut(s) 191
AciI CCGC 2 cut(s) 325, 932
AclWI GGATC 3 cut(s) 397, 773, 793
AcsI RAATTY 1 cut(s) 28
AfaI GTAC 3 cut(s) 540, 827, 959
AfiI CCNNNNNNNGG 3 cut(s) 105, 191, 209
AjnI CCWGG 3 cut(s) 207, 561, 964
AluBI AGCT 6 cut(s) 58, 345, 586, 610, 730, 907
AluI AGCT 6 cut(s) 58, 345, 586, 610, 730, 907
Alw26I GTCTC 3 cut(s) 400, 455, 897
AlwI GGATC 3 cut(s) 397, 773, 793
Ama87I CYCGRG 2 cut(s) 681, 898
AoxI GGCC 1 cut(s) 434
ApeKI GCWGC 3 cut(s) 586, 640, 730
ApoI RAATTY 1 cut(s) 28
AspLEI GCGC 1 cut(s) 678
AspS9I GGNCC 1 cut(s) 435
AsuC2I CCSGG 2 cut(s) 682, 683
AsuHPI GGTGA 2 cut(s) 85, 855
AvaI CYCGRG 2 cut(s) 681, 898
BanI GGYRCC 1 cut(s) 136
BanII GRGCYC 1 cut(s) 420
BbsI GAAGAC 1 cut(s) 589
BbvI GCAGC 3 cut(s) 573, 627, 717
BccI CCATC 1 cut(s) 110
BciT130I CCWGG 3 cut(s) 209, 563, 966
BciVI GTATCC 1 cut(s) 81
BclI TGATCA 1 cut(s) 985
BcnI CCSGG 2 cut(s) 682, 683
BcoDI GTCTC 3 cut(s) 400, 455, 897
BfaI CTAG 2 cut(s) 165, 882
BfmI CTRYAG 1 cut(s) 641
BfuAI ACCTGC 1 cut(s) 340
BfuI GTATCC 1 cut(s) 81
BisI GCNGC 3 cut(s) 587, 641, 731
BlsI GCNGC 3 cut(s) 588, 642, 732
Bme1390I CCNGG 5 cut(s) 209, 563, 682, 683, 966
BmeT110I CYCGRG 2 cut(s) 681, 898
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 2 cut(s) 138, 648
BmrFI CCNGG 5 cut(s) 209, 563, 682, 683, 966
BmsI GCATC 3 cut(s) 254, 627, 869
BpiI GAAGAC 1 cut(s) 589
BplI GAGNNNNNCTC 4 cut(s) 434, 466, 896, 928
BpuMI CCSGG 2 cut(s) 682, 683
BsaJI CCNNGG 3 cut(s) 208, 681, 802
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 191, 209
Bse1I ACTGG 2 cut(s) 185, 280
Bse3DI GCAATG 2 cut(s) 62, 669
BseBI CCWGG 3 cut(s) 209, 563, 966
BseDI CCNNGG 3 cut(s) 208, 681, 802
BseGI GGATG 2 cut(s) 121, 730
BseLI CCNNNNNNNGG 3 cut(s) 105, 191, 209
BseMI GCAATG 2 cut(s) 62, 669
BseMII CTCAG 1 cut(s) 465
BseNI ACTGG 2 cut(s) 185, 280
BseXI GCAGC 3 cut(s) 573, 627, 717
BsgI GTGCAG 1 cut(s) 572
BshFI GGCC 1 cut(s) 436
BshNI GGYRCC 1 cut(s) 136
BsiHKCI CYCGRG 2 cut(s) 681, 898
BsiSI CCGG 1 cut(s) 682
BslI CCNNNNNNNGG 3 cut(s) 105, 191, 209
BsmAI GTCTC 3 cut(s) 400, 455, 897
BsmBI CGTCTC 1 cut(s) 897
BsnI GGCC 1 cut(s) 436
BsoBI CYCGRG 2 cut(s) 681, 898
Bsp1286I GDGCHC 1 cut(s) 420
Bsp1407I TGTACA 2 cut(s) 825, 957
Bsp143I GATC 5 cut(s) 402, 497, 778, 798, 985
Bsp19I CCATGG 1 cut(s) 802
BspACI CCGC 2 cut(s) 325, 932
BspANI GGCC 1 cut(s) 436
BspCNI CTCAG 1 cut(s) 464
BspLI GGNNCC 2 cut(s) 138, 648
BspMAI CTGCAG 1 cut(s) 645
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 3 cut(s) 397, 773, 793
BspT107I GGYRCC 1 cut(s) 136
BsrDI GCAATG 2 cut(s) 62, 669
BsrGI TGTACA 2 cut(s) 825, 957
BsrI ACTGG 2 cut(s) 185, 280
BssECI CCNNGG 3 cut(s) 208, 681, 802
BssMI GATC 5 cut(s) 402, 497, 778, 798, 985
BssT1I CCWWGG 1 cut(s) 802
Bst2UI CCWGG 3 cut(s) 209, 563, 966
Bst4CI ACNGT 5 cut(s) 427, 617, 751, 767, 982
Bst6I CTCTTC 2 cut(s) 627, 662
BstAPI GCANNNNNTGC 1 cut(s) 176
BstAUI TGTACA 2 cut(s) 825, 957
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 2 cut(s) 451, 867
BstDSI CCRYGG 1 cut(s) 802
BstF5I GGATG 2 cut(s) 121, 730
BstHHI GCGC 1 cut(s) 678
BstKTI GATC 5 cut(s) 405, 500, 781, 801, 988
BstMAI GTCTC 3 cut(s) 400, 455, 897
BstMBI GATC 5 cut(s) 402, 497, 778, 798, 985
BstMWI GCNNNNNNNGC 5 cut(s) 64, 176, 586, 646, 913
BstNI CCWGG 3 cut(s) 209, 563, 966
BstNSI RCATGY 2 cut(s) 958, 964
BstSCI CCNGG 5 cut(s) 207, 561, 680, 681, 964
BstSFI CTRYAG 1 cut(s) 641
BstV1I GCAGC 3 cut(s) 573, 627, 717
BstV2I GAAGAC 1 cut(s) 589
BstX2I RGATCY 1 cut(s) 798
BstYI RGATCY 1 cut(s) 798
BsuI GTATCC 1 cut(s) 81
BsuRI GGCC 1 cut(s) 436
BtgI CCRYGG 1 cut(s) 802
BtsCI GGATG 2 cut(s) 121, 730
BtsI GCAGTG 1 cut(s) 92
BtsIMutI CAGTG 1 cut(s) 92
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 1 cut(s) 60
CfoI GCGC 1 cut(s) 678
Cfr13I GGNCC 1 cut(s) 435
Cfr9I CCCGGG 1 cut(s) 681
CseI GACGC 1 cut(s) 960
Csp6I GTAC 3 cut(s) 539, 826, 958
CspCI CAANNNNNGTGG 2 cut(s) 204, 239
CviAII CATG 4 cut(s) 803, 845, 955, 961
CviQI GTAC 3 cut(s) 539, 826, 958
DdeI CTNAG 2 cut(s) 451, 867
DpnI GATC 5 cut(s) 404, 499, 780, 800, 987
DpnII GATC 5 cut(s) 402, 497, 778, 798, 985
Eam1104I CTCTTC 2 cut(s) 627, 662
EarI CTCTTC 2 cut(s) 627, 662
Eco130I CCWWGG 1 cut(s) 802
Eco24I GRGCYC 1 cut(s) 420
Eco88I CYCGRG 2 cut(s) 681, 898
EcoRII CCWGG 3 cut(s) 207, 561, 964
EcoT14I CCWWGG 1 cut(s) 802
EcoT38I GRGCYC 1 cut(s) 420
ErhI CCWWGG 1 cut(s) 802
Esp3I CGTCTC 1 cut(s) 897
FaeI CATG 4 cut(s) 806, 848, 958, 964
FatI CATG 4 cut(s) 802, 844, 954, 960
FbaI TGATCA 1 cut(s) 985
Fnu4HI GCNGC 3 cut(s) 587, 641, 731
FokI GGATG 2 cut(s) 128, 737
FriOI GRGCYC 1 cut(s) 420
Fsp4HI GCNGC 3 cut(s) 587, 641, 731
FspBI CTAG 2 cut(s) 165, 882
GlaI GCGC 1 cut(s) 677
GluI GCNGC 3 cut(s) 587, 641, 731
HaeIII GGCC 1 cut(s) 436
HapII CCGG 1 cut(s) 682
HgaI GACGC 1 cut(s) 960
HhaI GCGC 1 cut(s) 678
Hin1II CATG 4 cut(s) 806, 848, 958, 964
Hin6I GCGC 1 cut(s) 676
HinP1I GCGC 1 cut(s) 676
HindIII AAGCTT 2 cut(s) 343, 608
HinfI GANTC 4 cut(s) 125, 533, 848, 935
HpaII CCGG 1 cut(s) 682
HphI GGTGA 2 cut(s) 85, 855
Hpy166II GTNNAC 2 cut(s) 842, 950
Hpy188I TCNGA 6 cut(s) 87, 130, 148, 454, 695, 717
Hpy188III TCNNGA 2 cut(s) 165, 882
Hpy8I GTNNAC 2 cut(s) 842, 950
HpyCH4III ACNGT 5 cut(s) 427, 617, 751, 767, 982
HpyCH4IV ACGT 1 cut(s) 594
HpyCH4V TGCA 9 cut(s) 62, 67, 215, 245, 272, 476, 556, 589, 643
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 176, 586, 646, 913
HpyF3I CTNAG 2 cut(s) 451, 867
HpySE526I ACGT 1 cut(s) 594
Hsp92II CATG 4 cut(s) 806, 848, 958, 964
HspAI GCGC 1 cut(s) 676
Ksp22I TGATCA 1 cut(s) 985
Kzo9I GATC 5 cut(s) 402, 497, 778, 798, 985
LmnI GCTCC 3 cut(s) 55, 646, 904
Lsp1109I GCAGC 3 cut(s) 573, 627, 717
LweI GCATC 3 cut(s) 254, 627, 869
MaeI CTAG 2 cut(s) 165, 882
MaeII ACGT 1 cut(s) 594
MaeIII GTNAC 2 cut(s) 478, 761
MalI GATC 5 cut(s) 404, 499, 780, 800, 987
MboI GATC 5 cut(s) 402, 497, 778, 798, 985
MboII GAAGA 6 cut(s) 240, 288, 589, 644, 679, 692
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 1 cut(s) 798
MhlI GDGCHC 1 cut(s) 420
MluCI AATT 6 cut(s) 28, 171, 195, 300, 465, 471
MmeI TCCRAC 1 cut(s) 806
MnlI CCTC 5 cut(s) 91, 260, 426, 523, 701
MseI TTAA 4 cut(s) 347, 410, 494, 603
MslI CAYNNNNRTG 1 cut(s) 959
MspI CCGG 1 cut(s) 682
MspR9I CCNGG 5 cut(s) 209, 563, 682, 683, 966
MunI CAATTG 1 cut(s) 465
MvaI CCWGG 3 cut(s) 209, 563, 966
MwoI GCNNNNNNNGC 5 cut(s) 64, 176, 586, 646, 913
NciI CCSGG 2 cut(s) 682, 683
NcoI CCATGG 1 cut(s) 802
NdeII GATC 5 cut(s) 402, 497, 778, 798, 985
NlaIII CATG 4 cut(s) 806, 848, 958, 964
NlaIV GGNNCC 2 cut(s) 138, 648
NmuCI GTSAC 1 cut(s) 478
NspI RCATGY 2 cut(s) 958, 964
PaqCI CACCTGC 1 cut(s) 340
PfeI GAWTC 4 cut(s) 125, 533, 848, 935
PflMI CCANNNNNTGG 1 cut(s) 191
PkrI GCNGC 3 cut(s) 588, 642, 732
Psp6I CCWGG 3 cut(s) 207, 561, 964
PspGI CCWGG 3 cut(s) 207, 561, 964
PspN4I GGNNCC 2 cut(s) 138, 648
PspPI GGNCC 1 cut(s) 435
PstI CTGCAG 1 cut(s) 645
PsuI RGATCY 1 cut(s) 798
RsaI GTAC 3 cut(s) 540, 827, 959
RsaNI GTAC 3 cut(s) 539, 826, 958
RseI CAYNNNNRTG 1 cut(s) 959
SaqAI TTAA 4 cut(s) 347, 410, 494, 603
SatI GCNGC 3 cut(s) 587, 641, 731
Sau3AI GATC 5 cut(s) 402, 497, 778, 798, 985
Sau96I GGNCC 1 cut(s) 435
ScrFI CCNGG 5 cut(s) 209, 563, 682, 683, 966
SduI GDGCHC 1 cut(s) 420
SfaNI GCATC 3 cut(s) 254, 627, 869
SfcI CTRYAG 1 cut(s) 641
SmaI CCCGGG 1 cut(s) 683
SmiMI CAYNNNNRTG 1 cut(s) 959
Sse9I AATT 6 cut(s) 28, 171, 195, 300, 465, 471
SsiI CCGC 2 cut(s) 325, 932
SspMI CTAG 2 cut(s) 165, 882
StyD4I CCNGG 5 cut(s) 207, 561, 680, 681, 964
StyI CCWWGG 1 cut(s) 802
TaaI ACNGT 5 cut(s) 427, 617, 751, 767, 982
TaiI ACGT 1 cut(s) 597
TasI AATT 6 cut(s) 28, 171, 195, 300, 465, 471
TatI WGTACW 2 cut(s) 825, 957
TfiI GAWTC 4 cut(s) 125, 533, 848, 935
Tru1I TTAA 4 cut(s) 347, 410, 494, 603
Tru9I TTAA 4 cut(s) 347, 410, 494, 603
TscAI CASTG 1 cut(s) 99
TseFI GTSAC 1 cut(s) 478
TseI GCWGC 3 cut(s) 586, 640, 730
Tsp45I GTSAC 1 cut(s) 478
TspDTI ATGAA 5 cut(s) 249, 381, 589, 669, 680
TspGWI ACGGA 1 cut(s) 957
TspMI CCCGGG 1 cut(s) 681
TspRI CASTG 1 cut(s) 99
Van91I CCANNNNNTGG 1 cut(s) 191
XapI RAATTY 1 cut(s) 28
XbaI TCTAGA 2 cut(s) 164, 881
XceI RCATGY 2 cut(s) 958, 964
XmaI CCCGGG 1 cut(s) 681
XspI CTAG 2 cut(s) 165, 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.