Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0006045

Physical Location & Seq

Genomic Coordinates Forward (+)

42555599 .. 42564997

Gene Structure 9399 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00197890.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2451 bp

ATGGCTGACGAGCCCTCAGTGGCACCAACGCAACCGCCTTCTCCTTCATCCTCCAATTCGCCCCTCGGAAACTCTGTGATCCCCATAGTGAACAAGCTCCAGGACATCTTCGCGCAGCTCGGTTCCCAATCCACCATCGAGCTCCCTCAGGTCGCCGTCGTCGGCAGCCAGAGTAGCGGCAAGTCCAGTGTGCTCGAGGCCCTTGTAGGCCGCGACTTCTTGCCCCGTGGCTCTGAAATCTGCACGCGCCGTCCTCTCGTGCTCCAGCTCTTACAGACTAAGCGCAACGCCGATGGCACCGATGAGGAGTACGGCGAGTTCCTTCACGTGCCTGGAAAGCGCTTCTACGATTTCTCAGATATTAGAAGGGAAATTCAGGCTGAGACTGAGAGGGAAGCAGGTGGAAACAAAGGTGTCACAGACAAGCAGATTCGTTTGAAGATATTTTCTCCAAATGTTCTTGATATCACACTGGTAGATCTACCTGGAATTACAAAGGTTCCCGTTGGTGACCAGCCCTCTGATATTGAAGCACGGATTAGGACAATGATCATGTCTTACATCAAAATTCCAAGTTGTTTAATTCTAGCTGTCACAGCAGCAAATTCGGATTTAGCAAATTCAGATGCTCTTCAGATTGCAGGGAATGCTGATCCTGATGGTAAAATCTGCAACTTTATACTGTTATTCCGGGTTGAATTGGTAATTTATATTTATGTGACTCACATTAGTGAATTTCTTATGACAGGTCTCCGAACCATAGGAGTAGTCACAAAGCTGGATATTATGGATAGAGGTACTGATGCTCGTAATCTTTTGCTCGGAAAAGTTATCCCTCTACGACTAGGCTACATAGGTGTTGTGAATCGTAGTCAAGAGGTAACCCATCTTAAACTCTATAAAAGGTGCTTCTTCCTCTGCTGTATGTCTGATGATGGTGATAAACCAAGTGATTCTTTACTAGAAACAAACATGCTTTTGGATATTATATTGAACCGGAGTATCAAAGATGCACTTGTAGCTGAGGAAAAGTTTTTCCGCAGCCGCCCAGTATATAATGGTCTAGCTGATCGCTGCGGCATCCCTCAGTTGGCAAAGAAGTTGAATCAGATTCTAGTGCAACATATCAAGGCAGTACTCCCGGGGCTAAAATCACGCATAAGCTCCGCACTAGTTTCTGTTGCCAAGGAACATGCCAGTTATGGGGAAATCACAGAATCAAAGGCTGGTCAGGGAGCTCTTCTTCTTAACATTCTTTCAAAGTACTCAGAAGCATTTTCTACAATGGTCGAGGGTAAAAATGAAGAAATGTCAACAACTGAGCTGTCTGGCGGAGCACGAATTCACTATATCTTCCAGAACATCTTTGTGAAAAGTTTAGAGGAGGTGGATCCCTGTGAGGACCTAACAGATGATGACATTAGAACTGCCATTCAAAATGCGACGGGTCCTAAATCTGCATTATTTGTACCAGAGGTGCCTTTTGAAAATCTGGTCAGGAGGCAAATAGCTCGTTTGTTAGATCCCAGCCTTCAATGTGCCAGGTTCATATATGATGAGCTGATGAAGATTAGCCATCGCTGCCTTGTAAATGAATTGCAGCGATTCCCTGTTTTGAGAAAGCGGATGGATGAAGTTATAGGGAACTTTTTACGAGAAGGCCTTGAACCCTCTGAGACAATGATTGGACATATTATTGAGATGGAGATGGACTACATAAATACTTCACATGCTAATTTTATTGGTGGAAGTAAGGCCGTTGAGAGTGCATTGCAACAGGTGAAGTCCTCTCGGATTCCTGTGCCTCTTGCCAGGCAAAAGGATGGTGTAGACTCTGACAAGGCACCATCATCTGAAAGAAGTTTGAAGTCCAGAGCAATTCTTGGCAGACAAGTGAATGGAATTTTGCCCGACCAGGTATGCAAAAGTTGTCTCTTTGCAACAGCAACTCCTGCACTTCCCCCAACTGTCTTGAAGCCCACAGAAAACCACTCAGAGCAGGAGACAATTGAAATTGCGGTCACAAAACTTCTGTTAAGATCATACTATGACATTGTTAGGAAGAATATAGAGGATTCTGTACCAAAAGCGATAATGCACTTCTTGGAACCTGATGAAGTATCAATGAAGAGAAAGCGCACCCGGGAGACCCTTCGAGTTCTGCAACAGGCTTTCCGGACATTGGATGAGCTGCCTTTGGAAGCAGAAACAGTTGAGAAAGGTTTTACTATGGGTGCTGATACGACAGGGTTGCCAAAGATCCATGGATTGCATACATCGTCGATGTACTCCACAGGCAGTCCGAATGACTCTTACTCTTCACCTAAGAACCCAAAGTCCAGAAAGTCATCTCACTCTGGAGAGCTCAACTCACCGTTTTATGCAAATGCAGATTCTAATGGAAGTGGGCGCATGTACATGCCTGGTCTATATCCAACTGTTGATCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0008017 GO:0008092 GO:0015631 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0042579 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko04139 ko04214 ko04217 ko04621 ko04668 map04139 map04214 map04217 map04621 map04668

Pfam Domains

Protein Families

Dynamin_M Dynamin_N GED

Protein Analysis

816

Amino Acids

90.21

Weight (kDa)

6.24

Isoelectric Point (pI)

56.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Dynamin_N	PF00350	51 - 228	8.1e-51	Dynamin family
Dynamin_M	PF01031	328 - 588	6.9e-90	Dynamin central region
GED	PF02212	667 - 702	3.9e-08	Dynamin GTPase effector domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006045)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G14120 AT2G14120 AT2G14120 AT2G14120 AT4G33650 AT4G33650
fragaria_vesca	FvH4_2g11510 FvH4_2g11510 FvH4_2g11510 FvH4_2g11510
malus_domestica	MD05G1049000.v1.1 MD10G1055800.v1.1
prunus_persica	Prupe.8G071400_v2.0.a1 Prupe.8G071400_v2.0.a1
pyrus_communis	pycom05g04070 pycom05g04080 pycom10g04290
rosa_chinensis	RchiOBHm_Chr6g0270491
rosa_laevigata	RLG00000013786
rosa_multiflora	Rmu_co8311497.1_g000001 Rmu_sc0009291.1_g000006 Rmu_sc0021982.1_g000001
rosa_roxburghii	Rroxscaffold_7G00197890
rosa_rugosa	Rorug06G0054900
rosa_samantha	Rh6CG173300 Rh6DG165800
rosa_wichuraiana	Rw6G014820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	389
Acc36I	ACCTGC	1 cut(s)	389
AccB1I	GGYRCC	4 cut(s)	22, 296, 1477, 1843
AccI	GTMKAC	1 cut(s)	1830
AccII	CGCG	3 cut(s)	113, 213, 247
AccIII	TCCGGA	1 cut(s)	2175
AclWI	GGATC	6 cut(s)	73, 647, 1385, 1398, 1517, 2254
AcsI	RAATTY	7 cut(s)	372, 567, 604, 619, 734, 1341, 1902
AcuI	CTGAAG	1 cut(s)	617
AcvI	CACGTG	1 cut(s)	328
AfaI	GTAC	8 cut(s)	311, 799, 1137, 1265, 1470, 2082, 2288, 2417
AfeI	AGCGCT	1 cut(s)	341
AfiI	CCNNNNNNNGG	3 cut(s)	1090, 1203, 2182
AhlI	ACTAGT	1 cut(s)	1171
AjnI	CCWGG	7 cut(s)	99, 331, 484, 1541, 1811, 1914, 2422
AleI	CACNNNNGTG	1 cut(s)	729
AloI	GAACNNNNNNTCC	2 cut(s)	2321, 2353
Alw21I	GWGCWC	6 cut(s)	144, 195, 264, 1240, 1339, 2367
Alw26I	GTCTC	6 cut(s)	377, 755, 1670, 1937, 1997, 2141
AlwI	GGATC	6 cut(s)	73, 647, 1385, 1398, 1517, 2254
Ama87I	CYCGRG	3 cut(s)	194, 1141, 2142
Aor13HI	TCCGGA	1 cut(s)	2175
Aor51HI	AGCGCT	1 cut(s)	341
AoxI	GGCC	4 cut(s)	198, 208, 1660, 1755
ApeKI	GCWGC	8 cut(s)	115, 165, 599, 1041, 1074, 1581, 1600, 2191
ApoI	RAATTY	7 cut(s)	372, 567, 604, 619, 734, 1341, 1902
AspLEI	GCGC	6 cut(s)	115, 249, 285, 342, 2139, 2412
AspS9I	GGNCC	3 cut(s)	199, 1402, 1448
AsuC2I	CCSGG	5 cut(s)	692, 1142, 1143, 2143, 2144
AsuHPI	GGTGA	5 cut(s)	521, 950, 1792, 2313, 2364
AvaI	CYCGRG	3 cut(s)	194, 1141, 2142
AvaII	GGWCC	2 cut(s)	1402, 1448
AxyI	CCTNAGG	1 cut(s)	147
BaeI	ACNNNNGTAYC	2 cut(s)	985, 1018
BamHI	GGATCC	1 cut(s)	1390
BanI	GGYRCC	4 cut(s)	22, 296, 1477, 1843
BanII	GRGCYC	4 cut(s)	15, 144, 1240, 2367
BauI	CACGAG	1 cut(s)	257
BbrPI	CACGTG	1 cut(s)	328
Bbv12I	GWGCWC	6 cut(s)	144, 195, 264, 1240, 1339, 2367
BbvCI	CCTCAGC	1 cut(s)	1023
BbvI	GCAGC	8 cut(s)	127, 177, 611, 1053, 1061, 1568, 1612, 2178
BceAI	ACGGC	4 cut(s)	140, 234, 328, 1742
BcgI	CGANNNNNNTGC	6 cut(s)	588, 622, 1493, 1527, 2233, 2267
BciT130I	CCWGG	7 cut(s)	101, 333, 486, 1543, 1813, 1916, 2424
BclI	TGATCA	1 cut(s)	549
BcnI	CCSGG	5 cut(s)	692, 1142, 1143, 2143, 2144
BcoDI	GTCTC	6 cut(s)	377, 755, 1670, 1937, 1997, 2141
BcuI	ACTAGT	1 cut(s)	1171
BfaI	CTAG	6 cut(s)	587, 845, 962, 1064, 1115, 1172
BfoI	RGCGCY	1 cut(s)	343
BfuAI	ACCTGC	1 cut(s)	389
BglII	AGATCT	1 cut(s)	478
BmcAI	AGTACT	2 cut(s)	1137, 1265
Bme18I	GGWCC	2 cut(s)	1402, 1448
BmeT110I	CYCGRG	3 cut(s)	194, 1141, 2142
BmgT120I	GGNCC	3 cut(s)	199, 1402, 1448
BmiI	GGNNCC	9 cut(s)	24, 124, 298, 501, 1392, 1449, 1479, 1845, 2109
BmrI	ACTGGG	1 cut(s)	1043
BmsI	GCATC	4 cut(s)	616, 793, 1000, 1089
BmuI	ACTGGG	1 cut(s)	1043
BplI	GAGNNNNNCTC	2 cut(s)	2354, 2386
BpmI	CTGGAG	3 cut(s)	83, 248, 2379
Bpu10I	CCTNAGC	1 cut(s)	1023
BpuMI	CCSGG	5 cut(s)	692, 1142, 1143, 2143, 2144
BsaAI	YACGTR	1 cut(s)	328
BsaI	GGTCTC	2 cut(s)	755, 2141
BsaJI	CCNNGG	7 cut(s)	64, 226, 1141, 1142, 1185, 2142, 2263
BsaWI	WCCGGW	2 cut(s)	996, 2175
BsaXI	ACNNNNNCTCC	2 cut(s)	1933, 1963
Bsc4I	CCNNNNNNNGG	3 cut(s)	1090, 1203, 2182
Bse1I	ACTGG	4 cut(s)	186, 477, 1049, 1197
Bse21I	CCTNAGG	1 cut(s)	147
Bse3DI	GCAATG	1 cut(s)	1769
BseAI	TCCGGA	1 cut(s)	2175
BseBI	CCWGG	7 cut(s)	101, 333, 486, 1543, 1813, 1916, 2424
BseDI	CCNNGG	7 cut(s)	64, 226, 1141, 1142, 1185, 2142, 2263
BseGI	GGATG	6 cut(s)	47, 1080, 1632, 1636, 1828, 2191
BseLI	CCNNNNNNNGG	3 cut(s)	1090, 1203, 2182
BseMI	GCAATG	1 cut(s)	1769
BseNI	ACTGG	4 cut(s)	186, 477, 1049, 1197
BseRI	GAGGAG	2 cut(s)	320, 1397
BseXI	GCAGC	8 cut(s)	127, 177, 611, 1053, 1061, 1568, 1612, 2178
BseYI	CCCAGC	1 cut(s)	1526
BsgI	GTGCAG	2 cut(s)	226, 1938
Bsh1236I	CGCG	3 cut(s)	113, 213, 247
BshFI	GGCC	4 cut(s)	200, 210, 1662, 1757
BshNI	GGYRCC	4 cut(s)	22, 296, 1477, 1843
BsiHKAI	GWGCWC	6 cut(s)	144, 195, 264, 1240, 1339, 2367
BsiHKCI	CYCGRG	3 cut(s)	194, 1141, 2142
BsiSI	CCGG	5 cut(s)	691, 997, 1142, 2143, 2176
BslI	CCNNNNNNNGG	3 cut(s)	1090, 1203, 2182
BsmAI	GTCTC	6 cut(s)	377, 755, 1670, 1937, 1997, 2141
BsmI	GAATGC	1 cut(s)	652
BsnI	GGCC	4 cut(s)	200, 210, 1662, 1757
Bso31I	GGTCTC	2 cut(s)	755, 2141
BsoBI	CYCGRG	3 cut(s)	194, 1141, 2142
Bsp1286I	GDGCHC	7 cut(s)	15, 144, 195, 264, 1240, 1339, 2367
Bsp13I	TCCGGA	1 cut(s)	2175
Bsp1407I	TGTACA	1 cut(s)	2415
Bsp19I	CCATGG	1 cut(s)	2263
BspANI	GGCC	4 cut(s)	200, 210, 1662, 1757
BspEI	TCCGGA	1 cut(s)	2175
BspFNI	CGCG	3 cut(s)	113, 213, 247
BspLI	GGNNCC	9 cut(s)	24, 124, 298, 501, 1392, 1449, 1479, 1845, 2109
BspMI	ACCTGC	1 cut(s)	389
BspPI	GGATC	6 cut(s)	73, 647, 1385, 1398, 1517, 2254
BspQI	GCTCTTC	2 cut(s)	636, 1245
BspT107I	GGYRCC	4 cut(s)	22, 296, 1477, 1843
BspTNI	GGTCTC	2 cut(s)	755, 2141
BsrDI	GCAATG	1 cut(s)	1769
BsrGI	TGTACA	1 cut(s)	2415
BsrI	ACTGG	4 cut(s)	186, 477, 1049, 1197
BssECI	CCNNGG	7 cut(s)	64, 226, 1141, 1142, 1185, 2142, 2263
BssSI	CACGAG	1 cut(s)	257
BssT1I	CCWWGG	2 cut(s)	1185, 2263
Bst2BI	CACGAG	1 cut(s)	257
Bst2UI	CCWGG	7 cut(s)	101, 333, 486, 1543, 1813, 1916, 2424
Bst4CI	ACNGT	5 cut(s)	684, 1969, 2212, 2376, 2440
Bst6I	CTCTTC	4 cut(s)	636, 1245, 2123, 2323
BstAPI	GCANNNNNTGC	2 cut(s)	647, 1952
BstAUI	TGTACA	1 cut(s)	2415
BstBAI	YACGTR	1 cut(s)	328
BstC8I	GCNNGC	1 cut(s)	245
BstDSI	CCRYGG	2 cut(s)	226, 2263
BstEII	GGTNACC	2 cut(s)	509, 880
BstF5I	GGATG	6 cut(s)	47, 1080, 1632, 1636, 1828, 2191
BstFNI	CGCG	3 cut(s)	113, 213, 247
BstH2I	RGCGCY	1 cut(s)	343
BstHHI	GCGC	6 cut(s)	115, 249, 285, 342, 2139, 2412
BstMAI	GTCTC	6 cut(s)	377, 755, 1670, 1937, 1997, 2141
BstMWI	GCNNNNNNNGC	7 cut(s)	174, 337, 596, 647, 1019, 1581, 1952
BstNI	CCWGG	7 cut(s)	101, 333, 486, 1543, 1813, 1916, 2424
BstNSI	RCATGY	5 cut(s)	976, 1196, 1733, 2416, 2422
BstPI	GGTNACC	2 cut(s)	509, 880
BstUI	CGCG	3 cut(s)	113, 213, 247
BstV1I	GCAGC	8 cut(s)	127, 177, 611, 1053, 1061, 1568, 1612, 2178
BstX2I	RGATCY	4 cut(s)	478, 1390, 1522, 2259
BstYI	RGATCY	4 cut(s)	478, 1390, 1522, 2259
Bsu36I	CCTNAGG	1 cut(s)	147
BsuRI	GGCC	4 cut(s)	200, 210, 1662, 1757
BtgI	CCRYGG	2 cut(s)	226, 2263
BtgZI	GCGATG	1 cut(s)	1562
BtsCI	GGATG	6 cut(s)	47, 1080, 1632, 1636, 1828, 2191
BtsIMutI	CAGTG	3 cut(s)	24, 193, 470
BveI	ACCTGC	1 cut(s)	389
Cac8I	GCNNGC	1 cut(s)	245
CfoI	GCGC	6 cut(s)	115, 249, 285, 342, 2139, 2412
Cfr13I	GGNCC	3 cut(s)	199, 1402, 1448
Cfr9I	CCCGGG	2 cut(s)	1141, 2142
CsiI	ACCWGGT	1 cut(s)	1914
Csp6I	GTAC	8 cut(s)	310, 798, 1136, 1264, 1469, 2081, 2287, 2416
CviAII	CATG	7 cut(s)	553, 973, 1193, 1730, 2264, 2413, 2419
CviQI	GTAC	8 cut(s)	310, 798, 1136, 1264, 1469, 2081, 2287, 2416
Eam1104I	CTCTTC	4 cut(s)	636, 1245, 2123, 2323
EarI	CTCTTC	4 cut(s)	636, 1245, 2123, 2323
EciI	GGCGGA	1 cut(s)	1347
Ecl136II	GAGCTC	3 cut(s)	142, 1238, 2365
Eco130I	CCWWGG	2 cut(s)	1185, 2263
Eco147I	AGGCCT	1 cut(s)	1662
Eco24I	GRGCYC	4 cut(s)	15, 144, 1240, 2367
Eco31I	GGTCTC	2 cut(s)	755, 2141
Eco32I	GATATC	1 cut(s)	466
Eco47I	GGWCC	2 cut(s)	1402, 1448
Eco47III	AGCGCT	1 cut(s)	341
Eco53kI	GAGCTC	3 cut(s)	142, 1238, 2365
Eco57I	CTGAAG	1 cut(s)	617
Eco72I	CACGTG	1 cut(s)	328
Eco81I	CCTNAGG	1 cut(s)	147
Eco88I	CYCGRG	3 cut(s)	194, 1141, 2142
Eco91I	GGTNACC	2 cut(s)	509, 880
EcoICRI	GAGCTC	3 cut(s)	142, 1238, 2365
EcoO109I	RGGNCCY	3 cut(s)	199, 1402, 1448
EcoO65I	GGTNACC	2 cut(s)	509, 880
EcoRI	GAATTC	1 cut(s)	1341
EcoRII	CCWGG	7 cut(s)	99, 331, 484, 1541, 1811, 1914, 2422
EcoRV	GATATC	1 cut(s)	466
EcoT14I	CCWWGG	2 cut(s)	1185, 2263
EcoT38I	GRGCYC	4 cut(s)	15, 144, 1240, 2367
ErhI	CCWWGG	2 cut(s)	1185, 2263
FaeI	CATG	7 cut(s)	556, 976, 1196, 1733, 2267, 2416, 2422
FalI	AAGNNNNNCTT	4 cut(s)	940, 972, 999, 1031
FatI	CATG	7 cut(s)	552, 972, 1192, 1729, 2263, 2412, 2418
FbaI	TGATCA	1 cut(s)	549
FblI	GTMKAC	1 cut(s)	1830
FokI	GGATG	6 cut(s)	34, 1067, 1639, 1643, 1835, 2198
FriOI	GRGCYC	4 cut(s)	15, 144, 1240, 2367
FspBI	CTAG	6 cut(s)	587, 845, 962, 1064, 1115, 1172
GlaI	GCGC	6 cut(s)	114, 248, 284, 341, 2138, 2411
GsaI	CCCAGC	1 cut(s)	1530
GsuI	CTGGAG	3 cut(s)	83, 248, 2379
HaeII	RGCGCY	1 cut(s)	343
HaeIII	GGCC	4 cut(s)	200, 210, 1662, 1757
HapII	CCGG	5 cut(s)	691, 997, 1142, 2143, 2176
HhaI	GCGC	6 cut(s)	115, 249, 285, 342, 2139, 2412
Hin1II	CATG	7 cut(s)	556, 976, 1196, 1733, 2267, 2416, 2422
Hin6I	GCGC	6 cut(s)	113, 247, 283, 340, 2137, 2410
HinP1I	GCGC	6 cut(s)	113, 247, 283, 340, 2137, 2410
HincII	GTYRAC	1 cut(s)	1314
HindII	GTYRAC	1 cut(s)	1314
HpaII	CCGG	5 cut(s)	691, 997, 1142, 2143, 2176
HphI	GGTGA	5 cut(s)	521, 950, 1792, 2313, 2364
Hpy166II	GTNNAC	3 cut(s)	91, 1314, 1831
Hpy8I	GTNNAC	3 cut(s)	91, 1314, 1831
Hpy99I	CGWCG	4 cut(s)	161, 164, 1447, 2284
HpyAV	CCTTC	7 cut(s)	48, 54, 332, 360, 1541, 1652, 2162
HpyCH4III	ACNGT	5 cut(s)	684, 1969, 2212, 2376, 2440
HpyCH4IV	ACGT	1 cut(s)	327
HpyF10VI	GCNNNNNNNGC	7 cut(s)	174, 337, 596, 647, 1019, 1581, 1952
HpySE526I	ACGT	1 cut(s)	327
Hsp92II	CATG	7 cut(s)	556, 976, 1196, 1733, 2267, 2416, 2422
HspAI	GCGC	6 cut(s)	113, 247, 283, 340, 2137, 2410
Kpn2I	TCCGGA	1 cut(s)	2175
Ksp22I	TGATCA	1 cut(s)	549
LguI	GCTCTTC	2 cut(s)	636, 1245
LmnI	GCTCC	6 cut(s)	102, 147, 267, 1169, 1235, 1334
Lsp1109I	GCAGC	8 cut(s)	127, 177, 611, 1053, 1061, 1568, 1612, 2178
LweI	GCATC	4 cut(s)	616, 793, 1000, 1089
MabI	ACCWGGT	1 cut(s)	1914
MaeI	CTAG	6 cut(s)	587, 845, 962, 1064, 1115, 1172
MaeII	ACGT	1 cut(s)	327
MaeIII	GTNAC	7 cut(s)	415, 509, 592, 718, 769, 880, 2020
MfeI	CAATTG	1 cut(s)	2007
MflI	RGATCY	4 cut(s)	478, 1390, 1522, 2259
MhlI	GDGCHC	7 cut(s)	15, 144, 195, 264, 1240, 1339, 2367
MlyI	GAGTC	3 cut(s)	715, 1826, 2303
MroI	TCCGGA	1 cut(s)	2175
MseI	TTAA	4 cut(s)	581, 891, 1248, 2036
MslI	CAYNNNNRTG	3 cut(s)	729, 1367, 2417
MspI	CCGG	5 cut(s)	691, 997, 1142, 2143, 2176
MunI	CAATTG	1 cut(s)	2007
Mva1269I	GAATGC	1 cut(s)	652
MvaI	CCWGG	7 cut(s)	101, 333, 486, 1543, 1813, 1916, 2424
MvnI	CGCG	3 cut(s)	113, 213, 247
MwoI	GCNNNNNNNGC	7 cut(s)	174, 337, 596, 647, 1019, 1581, 1952
NciI	CCSGG	5 cut(s)	692, 1142, 1143, 2143, 2144
NcoI	CCATGG	1 cut(s)	2263
NlaIII	CATG	7 cut(s)	556, 976, 1196, 1733, 2267, 2416, 2422
NlaIV	GGNNCC	9 cut(s)	24, 124, 298, 501, 1392, 1449, 1479, 1845, 2109
NmuCI	GTSAC	6 cut(s)	415, 509, 592, 718, 769, 2020
NspI	RCATGY	5 cut(s)	976, 1196, 1733, 2416, 2422
OliI	CACNNNNGTG	1 cut(s)	729
PaeR7I	CTCGAG	1 cut(s)	194
PaqCI	CACCTGC	1 cut(s)	389
PceI	AGGCCT	1 cut(s)	1662
PciSI	GCTCTTC	2 cut(s)	636, 1245
PctI	GAATGC	1 cut(s)	652
PfoI	TCCNGGA	1 cut(s)	99
PleI	GAGTC	3 cut(s)	715, 1826, 2303
PmaCI	CACGTG	1 cut(s)	328
PmlI	CACGTG	1 cut(s)	328
PpsI	GAGTC	3 cut(s)	715, 1826, 2303
Ppu21I	YACGTR	1 cut(s)	328
PpuMI	RGGWCCY	2 cut(s)	1402, 1448
Psp124BI	GAGCTC	3 cut(s)	144, 1240, 2367
Psp5II	RGGWCCY	2 cut(s)	1402, 1448
Psp6I	CCWGG	7 cut(s)	99, 331, 484, 1541, 1811, 1914, 2422
PspCI	CACGTG	1 cut(s)	328
PspEI	GGTNACC	2 cut(s)	509, 880
PspFI	CCCAGC	1 cut(s)	1526
PspGI	CCWGG	7 cut(s)	99, 331, 484, 1541, 1811, 1914, 2422
PspN4I	GGNNCC	9 cut(s)	24, 124, 298, 501, 1392, 1449, 1479, 1845, 2109
PspPI	GGNCC	3 cut(s)	199, 1402, 1448
PspPPI	RGGWCCY	2 cut(s)	1402, 1448
PspXI	VCTCGAGB	1 cut(s)	194
PsrI	GAACNNNNNNTAC	2 cut(s)	302, 334
PsuI	RGATCY	4 cut(s)	478, 1390, 1522, 2259
RsaI	GTAC	8 cut(s)	311, 799, 1137, 1265, 1470, 2082, 2288, 2417
RsaNI	GTAC	8 cut(s)	310, 798, 1136, 1264, 1469, 2081, 2287, 2416
RseI	CAYNNNNRTG	3 cut(s)	729, 1367, 2417
SacI	GAGCTC	3 cut(s)	144, 1240, 2367
SapI	GCTCTTC	2 cut(s)	636, 1245
SaqAI	TTAA	4 cut(s)	581, 891, 1248, 2036
Sau96I	GGNCC	3 cut(s)	199, 1402, 1448
ScaI	AGTACT	2 cut(s)	1137, 1265
SchI	GAGTC	3 cut(s)	715, 1826, 2303
SduI	GDGCHC	7 cut(s)	15, 144, 195, 264, 1240, 1339, 2367
SexAI	ACCWGGT	1 cut(s)	1914
SfaNI	GCATC	4 cut(s)	616, 793, 1000, 1089
Sfr274I	CTCGAG	1 cut(s)	194
SinI	GGWCC	2 cut(s)	1402, 1448
SlaI	CTCGAG	1 cut(s)	194
SmaI	CCCGGG	2 cut(s)	1143, 2144
SmiMI	CAYNNNNRTG	3 cut(s)	729, 1367, 2417
SmlI	CTYRAG	1 cut(s)	194
SmoI	CTYRAG	1 cut(s)	194
SpeI	ACTAGT	1 cut(s)	1171
SseBI	AGGCCT	1 cut(s)	1662
SspMI	CTAG	6 cut(s)	587, 845, 962, 1064, 1115, 1172
SstI	GAGCTC	3 cut(s)	144, 1240, 2367
StuI	AGGCCT	1 cut(s)	1662
StyI	CCWWGG	2 cut(s)	1185, 2263
TaaI	ACNGT	5 cut(s)	684, 1969, 2212, 2376, 2440
TaiI	ACGT	1 cut(s)	330
TaqI	TCGA	5 cut(s)	138, 195, 1290, 2155, 2282
TatI	WGTACW	4 cut(s)	1135, 1263, 2286, 2415
TauI	GCSGC	4 cut(s)	180, 213, 1047, 1080
Tru1I	TTAA	4 cut(s)	581, 891, 1248, 2036
Tru9I	TTAA	4 cut(s)	581, 891, 1248, 2036
TscAI	CASTG	3 cut(s)	24, 193, 477
TseFI	GTSAC	6 cut(s)	415, 509, 592, 718, 769, 2020
TseI	GCWGC	8 cut(s)	115, 165, 599, 1041, 1074, 1581, 1600, 2191
Tsp45I	GTSAC	6 cut(s)	415, 509, 592, 718, 769, 2020
TspDTI	ATGAA	8 cut(s)	36, 1317, 1537, 1580, 1608, 1647, 2130, 2141
TspGWI	ACGGA	1 cut(s)	550
TspMI	CCCGGG	2 cut(s)	1141, 2142
TspRI	CASTG	3 cut(s)	24, 193, 477
VpaK11BI	GGWCC	2 cut(s)	1402, 1448
XapI	RAATTY	7 cut(s)	372, 567, 604, 619, 734, 1341, 1902
XceI	RCATGY	5 cut(s)	976, 1196, 1733, 2416, 2422
XhoI	CTCGAG	1 cut(s)	194
XmaI	CCCGGG	2 cut(s)	1141, 2142
XmiI	GTMKAC	1 cut(s)	1830
XspI	CTAG	6 cut(s)	587, 845, 962, 1064, 1115, 1172
ZrmI	AGTACT	2 cut(s)	1137, 1265

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.