MD10G1066000.v1.1

B-box zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015868
Physical Location & Seq
Reverse (-)
9030878 .. 9032583
1706 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1066000.v1.1.491

Sequence Viewer

Length: 627 bp
ATGAAGATCCGGTGCGACGTGTGTGACAAAGAAGAGGCAACTGTCTTCTGCTCTGCCGATGAAGCCGCTCTTTGTGACGTCTGCGATCGCCAAATTCACCACGCAAACAAGCTTGCCAGCAAACACAAGCGCTTCTCTCTTCTCCACCCAACTTTCAAGGACTCCCCTCTTTGTGATATCTGTCAGGAAAGGCGAGGATTTCTCTTTTGTCAAGAAGATAGAGCAATTCTCTGCAGGGAATGTGACCTTTCGATTCACAAATCGAACGAACACACACAGAAACACAGCAGGTTTCTGCTCCCCGGTGTTAAGCTTTCTGCAGCTTCTTCTTTGTATCCAACTTCATCAGCCTGCTCCAACTTCTCCGAAGTGATGACCGCAAAAACTAATGATGCAAGAGCCTCAAAGTCATCCACAAAGAGACAGAGAACTGTTTCGTCGAACGAAATCTTGAGTTCTTCTTCTCCTTCGTTAATGGAACAAACAATGTCATCTTCGTCATACAACAAGATAGAAGAGAATTATTGTTTCAGTGACAATGGTTCGGGTTCGACAAGCAGCATATCGAAGTACTTGATGGAGACGCTGCCCGGGTGGCATGTGGAAGACCTTCTTGATTTTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-B_box

Protein Analysis

209

Amino Acids

23.26

Weight (kDa)

6.44

Isoelectric Point (pI)

61.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 3 - 46 2.2e-07 B-box zinc finger
zf-B_box PF00643 55 - 92 7.1e-07 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015868)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39070
fragaria_vesca FvH4_2g10070
malus_domestica MD10G1066000.v1.1
prunus_persica Prupe.8G087900_v2.0.a1
pyrus_communis pycom05g04940 pycom10g05150
rosa_chinensis RchiOBHm_Chr6g0267001
rosa_laevigata RLG00000014052
rosa_multiflora Rmu_sc0017071.1_g000007
rosa_rugosa Rorug06G0028400
rosa_samantha Rh6AG149900 Rh6BG150800 Rh6CG146000 Rh6DG134900
rosa_wichuraiana Rw6G012980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 81
Acc36I ACCTGC 1 cut(s) 279
AccBSI CCGCTC 1 cut(s) 68
AciI CCGC 2 cut(s) 66, 378
AcsI RAATTY 1 cut(s) 93
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 1 cut(s) 572
AfeI AGCGCT 1 cut(s) 131
AflIII ACRYGT 1 cut(s) 18
AgsI TTSAA 1 cut(s) 157
AjiI CACGTC 1 cut(s) 19
AluBI AGCT 3 cut(s) 112, 313, 323
AluI AGCT 3 cut(s) 112, 313, 323
Alw26I GTCTC 2 cut(s) 415, 575
Ama87I CYCGRG 1 cut(s) 590
Aor51HI AGCGCT 1 cut(s) 131
ApeKI GCWGC 3 cut(s) 320, 558, 586
ApoI RAATTY 1 cut(s) 93
AsiSI GCGATCGC 1 cut(s) 88
Asp700I GAANNNNTTC 2 cut(s) 433, 609
AspLEI GCGC 1 cut(s) 132
AsuC2I CCSGG 3 cut(s) 303, 591, 592
AsuHPI GGTGA 1 cut(s) 89
AvaI CYCGRG 1 cut(s) 590
BbsI GAAGAC 2 cut(s) 37, 612
BbvI GCAGC 3 cut(s) 332, 570, 573
BccI CCATC 1 cut(s) 571
BciVI GTATCC 1 cut(s) 345
BcnI CCSGG 3 cut(s) 303, 591, 592
BcoDI GTCTC 2 cut(s) 415, 575
BfmI CTRYAG 2 cut(s) 232, 318
BfoI RGCGCY 1 cut(s) 133
BfuAI ACCTGC 1 cut(s) 279
BfuI GTATCC 1 cut(s) 345
BglI GCCNNNNNGGC 1 cut(s) 595
BisI GCNGC 4 cut(s) 66, 321, 559, 587
BlsI GCNGC 4 cut(s) 67, 322, 560, 588
BmcAI AGTACT 1 cut(s) 572
Bme1390I CCNGG 3 cut(s) 303, 591, 592
BmeT110I CYCGRG 1 cut(s) 590
BmgBI CACGTC 1 cut(s) 19
BmrFI CCNGG 3 cut(s) 303, 591, 592
BmsI GCATC 1 cut(s) 382
BpiI GAAGAC 2 cut(s) 37, 612
BplI GAGNNNNNCTC 4 cut(s) 186, 218, 213, 245
BpuEI CTTGAG 1 cut(s) 472
BpuMI CCSGG 3 cut(s) 303, 591, 592
BsaHI GRCGYC 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 301, 590
BsaWI WCCGGW 1 cut(s) 9
BseDI CCNNGG 2 cut(s) 301, 590
BseGI GGATG 1 cut(s) 410
BseXI GCAGC 3 cut(s) 332, 570, 573
Bsh1285I CGRYCG 1 cut(s) 88
BsiEI CGRYCG 1 cut(s) 88
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 3 cut(s) 10, 303, 591
BsmAI GTCTC 2 cut(s) 415, 575
BsmBI CGTCTC 1 cut(s) 575
BsoBI CYCGRG 1 cut(s) 590
Bsp143I GATC 2 cut(s) 6, 85
BspACI CCGC 2 cut(s) 66, 378
BspMAI CTGCAG 2 cut(s) 236, 322
BspMI ACCTGC 1 cut(s) 279
BsrBI CCGCTC 1 cut(s) 68
BssECI CCNNGG 2 cut(s) 301, 590
BssMI GATC 2 cut(s) 6, 85
BssNI GRCGYC 1 cut(s) 78
Bst4CI ACNGT 2 cut(s) 43, 433
Bst6I CTCTTC 3 cut(s) 27, 144, 510
BstACI GRCGYC 1 cut(s) 78
BstC8I GCNNGC 3 cut(s) 114, 118, 352
BstF5I GGATG 1 cut(s) 410
BstH2I RGCGCY 1 cut(s) 133
BstHHI GCGC 1 cut(s) 132
BstKTI GATC 2 cut(s) 9, 88
BstMAI GTCTC 2 cut(s) 415, 575
BstMBI GATC 2 cut(s) 6, 85
BstMCI CGRYCG 1 cut(s) 88
BstMWI GCNNNNNNNGC 2 cut(s) 62, 595
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 3 cut(s) 301, 589, 590
BstSFI CTRYAG 2 cut(s) 232, 318
BstV1I GCAGC 3 cut(s) 332, 570, 573
BstV2I GAAGAC 2 cut(s) 37, 612
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
BsuI GTATCC 1 cut(s) 345
BtrI CACGTC 1 cut(s) 19
BtsCI GGATG 1 cut(s) 410
BtsIMutI CAGTG 1 cut(s) 538
BveI ACCTGC 1 cut(s) 279
Cac8I GCNNGC 3 cut(s) 114, 118, 352
CfoI GCGC 1 cut(s) 132
Cfr9I CCCGGG 1 cut(s) 590
CseI GACGC 1 cut(s) 592
Csp6I GTAC 1 cut(s) 571
CviAII CATG 1 cut(s) 599
CviJI RGCY 6 cut(s) 65, 112, 313, 323, 350, 401
CviKI_1 RGCY 6 cut(s) 65, 112, 313, 323, 350, 401
CviQI GTAC 1 cut(s) 571
DpnI GATC 2 cut(s) 8, 87
DpnII GATC 2 cut(s) 6, 85
Eam1104I CTCTTC 3 cut(s) 27, 144, 510
EarI CTCTTC 3 cut(s) 27, 144, 510
Eco32I GATATC 1 cut(s) 178
Eco47III AGCGCT 1 cut(s) 131
Eco88I CYCGRG 1 cut(s) 590
EcoRV GATATC 1 cut(s) 178
Esp3I CGTCTC 1 cut(s) 575
FaeI CATG 1 cut(s) 602
FaiI YATR 4 cut(s) 502, 563, 600, 625
FalI AAGNNNNNCTT 3 cut(s) 54, 86, 597
FatI CATG 1 cut(s) 598
Fnu4HI GCNGC 4 cut(s) 66, 321, 559, 587
FokI GGATG 1 cut(s) 397
Fsp4HI GCNGC 4 cut(s) 66, 321, 559, 587
GlaI GCGC 1 cut(s) 131
GluI GCNGC 4 cut(s) 66, 321, 559, 587
HaeII RGCGCY 1 cut(s) 133
HapII CCGG 3 cut(s) 10, 303, 591
HgaI GACGC 1 cut(s) 592
HhaI GCGC 1 cut(s) 132
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 1 cut(s) 602
Hin6I GCGC 1 cut(s) 130
HinP1I GCGC 1 cut(s) 130
HindIII AAGCTT 2 cut(s) 110, 311
HinfI GANTC 2 cut(s) 161, 253
HpaII CCGG 3 cut(s) 10, 303, 591
HphI GGTGA 1 cut(s) 89
Hpy188I TCNGA 1 cut(s) 367
Hpy188III TCNNGA 4 cut(s) 185, 212, 451, 614
Hpy99I CGWCG 2 cut(s) 20, 442
HpyAV CCTTC 2 cut(s) 477, 620
HpyCH4III ACNGT 2 cut(s) 43, 433
HpyCH4IV ACGT 2 cut(s) 18, 78
HpyCH4V TGCA 3 cut(s) 234, 320, 395
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 595
HpySE526I ACGT 2 cut(s) 18, 78
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 1 cut(s) 602
HspAI GCGC 1 cut(s) 130
Kzo9I GATC 2 cut(s) 6, 85
LmnI GCTCC 2 cut(s) 303, 359
LpnPI CCDG 8 cut(s) 23, 130, 170, 220, 274, 316, 364, 604
Lsp1109I GCAGC 3 cut(s) 332, 570, 573
LweI GCATC 1 cut(s) 382
MaeII ACGT 2 cut(s) 18, 78
MaeIII GTNAC 4 cut(s) 23, 74, 242, 533
MalI GATC 2 cut(s) 8, 87
MbiI CCGCTC 1 cut(s) 68
MboI GATC 2 cut(s) 6, 85
MflI RGATCY 1 cut(s) 6
MluCI AATT 3 cut(s) 93, 225, 520
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 2 cut(s) 362, 381
MnlI CCTC 4 cut(s) 28, 177, 188, 412
MroXI GAANNNNTTC 2 cut(s) 433, 609
MseI TTAA 2 cut(s) 309, 473
MspI CCGG 3 cut(s) 10, 303, 591
MspR9I CCNGG 3 cut(s) 303, 591, 592
MwoI GCNNNNNNNGC 2 cut(s) 62, 595
NciI CCSGG 3 cut(s) 303, 591, 592
NdeII GATC 2 cut(s) 6, 85
NlaIII CATG 1 cut(s) 602
NmuCI GTSAC 4 cut(s) 23, 74, 242, 533
NspI RCATGY 1 cut(s) 602
PdmI GAANNNNTTC 2 cut(s) 433, 609
PfeI GAWTC 1 cut(s) 253
PkrI GCNGC 4 cut(s) 67, 322, 560, 588
Ple19I CGATCG 1 cut(s) 88
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
PstI CTGCAG 2 cut(s) 236, 322
PsuI RGATCY 1 cut(s) 6
PvuI CGATCG 1 cut(s) 88
RgaI GCGATCGC 1 cut(s) 88
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
SaqAI TTAA 2 cut(s) 309, 473
SatI GCNGC 4 cut(s) 66, 321, 559, 587
Sau3AI GATC 2 cut(s) 6, 85
ScaI AGTACT 1 cut(s) 572
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 3 cut(s) 303, 591, 592
SetI ASST 8 cut(s) 21, 81, 114, 249, 293, 315, 325, 612
SfaAI GCGATCGC 1 cut(s) 88
SfaNI GCATC 1 cut(s) 382
SfcI CTRYAG 2 cut(s) 232, 318
SgfI GCGATCGC 1 cut(s) 88
SmaI CCCGGG 1 cut(s) 592
SmlI CTYRAG 1 cut(s) 451
SmoI CTYRAG 1 cut(s) 451
Sse9I AATT 3 cut(s) 93, 225, 520
SsiI CCGC 2 cut(s) 66, 378
StyD4I CCNGG 3 cut(s) 301, 589, 590
TaaI ACNGT 2 cut(s) 43, 433
TaiI ACGT 2 cut(s) 21, 81
TaqI TCGA 5 cut(s) 251, 263, 440, 551, 566
TasI AATT 3 cut(s) 93, 225, 520
TatI WGTACW 1 cut(s) 570
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 2 cut(s) 309, 473
Tru9I TTAA 2 cut(s) 309, 473
TscAI CASTG 1 cut(s) 538
TseFI GTSAC 4 cut(s) 23, 74, 242, 533
TseI GCWGC 3 cut(s) 320, 558, 586
Tsp45I GTSAC 4 cut(s) 23, 74, 242, 533
TspDTI ATGAA 4 cut(s) 17, 75, 333, 612
TspMI CCCGGG 1 cut(s) 590
TspRI CASTG 1 cut(s) 538
XapI RAATTY 1 cut(s) 93
XceI RCATGY 1 cut(s) 602
XmaI CCCGGG 1 cut(s) 590
XmnI GAANNNNTTC 2 cut(s) 433, 609
ZraI GACGTC 1 cut(s) 79
ZrmI AGTACT 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.