Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0012522

Physical Location & Seq

Genomic Coordinates Forward (+)

12498622 .. 12499812

Gene Structure 1191 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1085100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1191 bp

ATGGTCGTTGCATCCCCATCTCCTGCACCCGCCCCCACTCTTTCCACCCTCTTCATCACCACCTCACTCCATGCATCATCCCCGCCGTCCAAGTACCCCATCGACTTCTCCCCGCCCTTAATCGCCATGGTCGTGGTCGTCGCCACCGCCTTCCTTATTGTCACTTACTCCCGCCTCATCTCCCGCCACGTCATCCCGCCAATCCTCCGCCTCCAACGACGCTGGCACCACCGCCGTCGCCGCTCCTACCTCCCATCATCCATCGGTGACATCGACTCCCCGCCCTACGACTCGTCGCTCGACCCCGCCGACATCGGCTTCCACGTGTTCTCCCCCTACGGCCTCGACGAGTCGGTGATCAAAACAATCCCCCTCTCTCTATACACATCAAAACAATCCCTCCTCAGCCGTGACTGCGCCGTTTGCCTGATCGACTTCCAAAACGACGACTACGTTCGAACGCTGCCGGTCTGCTCGCACGTGTTCCACGTTGACTGCATCGACATGTGGCTGCGATCTCACGCCAACTGTCCTCTCTGCCGGGCCGGAATATTCCGTCCGGAATCTCCATTCGTTCCGTTGATGGCCGCAAGGATCAGGCCCAGCCTCGACGACACTGTTTTCCGGAGCATCGCGCTCGAGCCTCTTGCCGAAGCTACGGCGCCGTGTGATTCCGATTTTTTAGTCACCGAGATCGAGAGGTGCGCGGAGGAGCGATCGCCGAGGAGGAGCTCCAACTACTGGGAGGATTACCGGATCAACGGCGGAGATTTTGTTTTAAAGAGGTCGCACTCGTTCGGATTCGAGCGGAGCTCGGCGTCGGCGGAGAGGATGATGGTGACGGAGCCTGCGACGGCATCACCGTGGCGGTACCGGAGAGGAAGCTTCTGGAGCAAGCGGCCGTCGCCGTTCGGGTCGTTGACGAAGCCTCGAGTCTTCTCCTTCAGATCATCCTACAGAGGCGGCGTCAGCATGAAATCGCCGTTCTTCCGCCGAAAAGGCTTCTTCCCGCTGTCAGAGTCGAGCGCGAGATTCTCCGGCGCCGGAACCGGTGGATATTCGCGGCGTAGCAAGTCGATGACAAGTCCGATGTTCGGAAGATCATCGGGGTTATCGGCTTTCTCATCGAGCCGGCTAAGGTGCGGAGATCCGGAGGCGCTGTTGTCGCCGGAGAGGTTTAACAGGAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

397

Amino Acids

44.44

Weight (kDa)

9.79

Isoelectric Point (pI)

82.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	138 - 181	1.1e-13	Ring finger domain
zf-RING_11	PF17123	138 - 167	8.2e-10	RING-like zinc finger
zf-C3HC4_2	PF13923	139 - 180	5.2e-07	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	148 - 181	1.9e-09	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012522)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18930 AT3G18930 AT3G18930
fragaria_vesca	FvH4_2g07470
malus_domestica	MD10G1085100.v1.1
prunus_persica	Prupe.8G108300_v2.0.a1
pyrus_communis	pycom10g06670
rosa_laevigata	RLG00000014460
rosa_multiflora	Rmu_co8088512.1_g000001 Rmu_co8388237.1_g000001 Rmu_sc0006019.1_g000006 Rmu_sc0015236.1_g000002
rosa_roxburghii	Rroxscaffold_7G00207740
rosa_rugosa	Rorug05G0594900
rosa_samantha	Rh6AG114300 Rh6BG108500 Rh6CG105900 Rh6DG096300
rosa_wichuraiana	Rw6G009800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	870
AccB1I	GGYRCC	4 cut(s)	225, 661, 870, 1040
AccB7I	CCANNNNNTGG	1 cut(s)	741
AccBSI	CCGCTC	2 cut(s)	243, 808
AccII	CGCG	4 cut(s)	635, 707, 1028, 1063
AccIII	TCCGGA	3 cut(s)	559, 624, 1150
AclWI	GGATC	3 cut(s)	602, 764, 1142
AcoI	YGGCCR	2 cut(s)	585, 899
AcuI	CTGAAG	1 cut(s)	928
AcvI	CACGTG	2 cut(s)	325, 481
AcyI	GRCGYC	4 cut(s)	662, 818, 966, 1041
AfaI	GTAC	2 cut(s)	95, 872
AfiI	CCNNNNNNNGG	2 cut(s)	741, 1094
AflIII	ACRYGT	3 cut(s)	324, 480, 504
AgeI	ACCGGT	1 cut(s)	1049
AjiI	CACGTC	1 cut(s)	190
AluBI	AGCT	4 cut(s)	656, 732, 813, 885
AluI	AGCT	4 cut(s)	656, 732, 813, 885
Alw21I	GWGCWC	2 cut(s)	734, 815
AlwI	GGATC	3 cut(s)	602, 764, 1142
Ama87I	CYCGRG	2 cut(s)	638, 930
Aor13HI	TCCGGA	3 cut(s)	559, 624, 1150
AoxI	GGCC	5 cut(s)	340, 543, 585, 599, 899
ApeKI	GCWGC	2 cut(s)	463, 511
AsiGI	ACCGGT	1 cut(s)	1049
AsiSI	GCGATCGC	1 cut(s)	719
Asp718I	GGTACC	1 cut(s)	870
AspLEI	GCGC	7 cut(s)	419, 637, 664, 707, 1028, 1043, 1159
AspS9I	GGNCC	2 cut(s)	543, 600
AsuC2I	CCSGG	1 cut(s)	542
AsuHPI	GGTGA	6 cut(s)	49, 278, 367, 679, 850, 852
AsuII	TTCGAA	1 cut(s)	457
AvaI	CYCGRG	2 cut(s)	638, 930
BanI	GGYRCC	4 cut(s)	225, 661, 870, 1040
BanII	GRGCYC	2 cut(s)	734, 815
BbrPI	CACGTG	2 cut(s)	325, 481
BbsI	GAAGAC	1 cut(s)	928
Bbv12I	GWGCWC	2 cut(s)	734, 815
BbvCI	CCTCAGC	1 cut(s)	404
BbvI	GCAGC	2 cut(s)	450, 498
BccI	CCATC	6 cut(s)	25, 107, 262, 269, 577, 829
BcgI	CGANNNNNNTGC	4 cut(s)	619, 629, 653, 663
BclI	TGATCA	1 cut(s)	357
BcnI	CCSGG	1 cut(s)	542
BfmI	CTRYAG	1 cut(s)	955
BfoI	RGCGCY	3 cut(s)	665, 1044, 1160
BglI	GCCNNNNNGGC	1 cut(s)	999
BisI	GCNGC	7 cut(s)	241, 464, 512, 588, 899, 964, 1064
BlsI	GCNGC	7 cut(s)	242, 465, 513, 589, 900, 965, 1065
Bme1390I	CCNGG	1 cut(s)	542
BmeT110I	CYCGRG	2 cut(s)	638, 930
BmgBI	CACGTC	1 cut(s)	190
BmgT120I	GGNCC	2 cut(s)	543, 600
BmiI	GGNNCC	6 cut(s)	227, 663, 846, 872, 1042, 1048
BmrFI	CCNGG	1 cut(s)	542
BmrI	ACTGGG	1 cut(s)	751
BmsI	GCATC	5 cut(s)	20, 83, 507, 639, 866
BmuI	ACTGGG	1 cut(s)	751
BpiI	GAAGAC	1 cut(s)	928
BplI	GAGNNNNNCTC	2 cut(s)	797, 829
BpmI	CTGGAG	1 cut(s)	910
Bpu10I	CCTNAGC	2 cut(s)	404, 1136
Bpu14I	TTCGAA	1 cut(s)	457
BpuMI	CCSGG	1 cut(s)	542
BsaAI	YACGTR	2 cut(s)	325, 481
BsaHI	GRCGYC	4 cut(s)	662, 818, 966, 1041
BsaJI	CCNNGG	3 cut(s)	126, 722, 863
BsaWI	WCCGGW	6 cut(s)	559, 624, 753, 873, 1049, 1150
Bsc4I	CCNNNNNNNGG	2 cut(s)	741, 1094
Bse118I	RCCGGY	3 cut(s)	466, 1049, 1131
Bse1I	ACTGG	1 cut(s)	746
BseAI	TCCGGA	3 cut(s)	559, 624, 1150
BseDI	CCNNGG	3 cut(s)	126, 722, 863
BseGI	GGATG	6 cut(s)	11, 77, 192, 257, 837, 950
BseLI	CCNNNNNNNGG	2 cut(s)	741, 1094
BseMII	CTCAG	1 cut(s)	418
BseNI	ACTGG	1 cut(s)	746
BseRI	GAGGAG	4 cut(s)	392, 725, 739, 742
BseX3I	CGGCCG	1 cut(s)	899
BseXI	GCAGC	2 cut(s)	450, 498
BseYI	CCCAGC	1 cut(s)	602
BsgI	GTGCAG	1 cut(s)	9
Bsh1236I	CGCG	4 cut(s)	635, 707, 1028, 1063
Bsh1285I	CGRYCG	2 cut(s)	719, 902
BshFI	GGCC	5 cut(s)	342, 545, 587, 601, 901
BshNI	GGYRCC	4 cut(s)	225, 661, 870, 1040
BshTI	ACCGGT	1 cut(s)	1049
BsiEI	CGRYCG	2 cut(s)	719, 902
BsiHKAI	GWGCWC	2 cut(s)	734, 815
BsiHKCI	CYCGRG	2 cut(s)	638, 930
BslI	CCNNNNNNNGG	2 cut(s)	741, 1094
BsnI	GGCC	5 cut(s)	342, 545, 587, 601, 901
BsoBI	CYCGRG	2 cut(s)	638, 930
Bsp119I	TTCGAA	1 cut(s)	457
Bsp1286I	GDGCHC	2 cut(s)	734, 815
Bsp13I	TCCGGA	3 cut(s)	559, 624, 1150
Bsp19I	CCATGG	1 cut(s)	126
BspANI	GGCC	5 cut(s)	342, 545, 587, 601, 901
BspCNI	CTCAG	1 cut(s)	417
BspEI	TCCGGA	3 cut(s)	559, 624, 1150
BspFNI	CGCG	4 cut(s)	635, 707, 1028, 1063
BspLI	GGNNCC	6 cut(s)	227, 663, 846, 872, 1042, 1048
BspPI	GGATC	3 cut(s)	602, 764, 1142
BspT104I	TTCGAA	1 cut(s)	457
BspT107I	GGYRCC	4 cut(s)	225, 661, 870, 1040
BsrBI	CCGCTC	2 cut(s)	243, 808
BsrFI	RCCGGY	3 cut(s)	466, 1049, 1131
BsrI	ACTGG	1 cut(s)	746
BssAI	RCCGGY	3 cut(s)	466, 1049, 1131
BssECI	CCNNGG	3 cut(s)	126, 722, 863
BssNI	GRCGYC	4 cut(s)	662, 818, 966, 1041
BssT1I	CCWWGG	1 cut(s)	126
Bst4CI	ACNGT	3 cut(s)	530, 619, 864
Bst6I	CTCTTC	1 cut(s)	56
BstACI	GRCGYC	4 cut(s)	662, 818, 966, 1041
BstBAI	YACGTR	2 cut(s)	325, 481
BstBI	TTCGAA	1 cut(s)	457
BstC8I	GCNNGC	5 cut(s)	224, 476, 849, 896, 1133
BstDEI	CTNAG	2 cut(s)	404, 1136
BstDSI	CCRYGG	2 cut(s)	126, 863
BstF5I	GGATG	6 cut(s)	11, 77, 192, 257, 837, 950
BstFNI	CGCG	4 cut(s)	635, 707, 1028, 1063
BstH2I	RGCGCY	3 cut(s)	665, 1044, 1160
BstHHI	GCGC	7 cut(s)	419, 637, 664, 707, 1028, 1043, 1159
BstMCI	CGRYCG	2 cut(s)	719, 902
BstMWI	GCNNNNNNNGC	8 cut(s)	240, 414, 423, 891, 904, 969, 999, 1165
BstNSI	RCATGY	1 cut(s)	508
BstSCI	CCNGG	1 cut(s)	540
BstSFI	CTRYAG	1 cut(s)	955
BstUI	CGCG	4 cut(s)	635, 707, 1028, 1063
BstV1I	GCAGC	2 cut(s)	450, 498
BstV2I	GAAGAC	1 cut(s)	928
BstX2I	RGATCY	1 cut(s)	1147
BstXI	CCANNNNNNTGG	1 cut(s)	133
BstYI	RGATCY	1 cut(s)	1147
BstZI	CGGCCG	1 cut(s)	899
BsuRI	GGCC	5 cut(s)	342, 545, 587, 601, 901
BtgI	CCRYGG	2 cut(s)	126, 863
BtgZI	GCGATG	1 cut(s)	616
BtrI	CACGTC	1 cut(s)	190
BtsCI	GGATG	6 cut(s)	11, 77, 192, 257, 837, 950
BtsIMutI	CAGTG	1 cut(s)	615
Cac8I	GCNNGC	5 cut(s)	224, 476, 849, 896, 1133
CfoI	GCGC	7 cut(s)	419, 637, 664, 707, 1028, 1043, 1159
Cfr10I	RCCGGY	3 cut(s)	466, 1049, 1131
Cfr13I	GGNCC	2 cut(s)	543, 600
CseI	GACGC	3 cut(s)	228, 807, 955
Csp6I	GTAC	2 cut(s)	94, 871
CspAI	ACCGGT	1 cut(s)	1049
CviAII	CATG	4 cut(s)	71, 127, 505, 973
CviQI	GTAC	2 cut(s)	94, 871
DdeI	CTNAG	2 cut(s)	404, 1136
DinI	GGCGCC	2 cut(s)	663, 1042
DraI	TTTAAA	1 cut(s)	780
EaeI	YGGCCR	2 cut(s)	585, 899
EagI	CGGCCG	1 cut(s)	899
Eam1104I	CTCTTC	1 cut(s)	56
EarI	CTCTTC	1 cut(s)	56
EciI	GGCGGA	4 cut(s)	197, 780, 839, 980
Ecl136II	GAGCTC	2 cut(s)	732, 813
EclXI	CGGCCG	1 cut(s)	899
Eco130I	CCWWGG	1 cut(s)	126
Eco24I	GRGCYC	2 cut(s)	734, 815
Eco52I	CGGCCG	1 cut(s)	899
Eco53kI	GAGCTC	2 cut(s)	732, 813
Eco57I	CTGAAG	1 cut(s)	928
Eco72I	CACGTG	2 cut(s)	325, 481
Eco88I	CYCGRG	2 cut(s)	638, 930
EcoICRI	GAGCTC	2 cut(s)	732, 813
EcoT14I	CCWWGG	1 cut(s)	126
EcoT22I	ATGCAT	1 cut(s)	76
EcoT38I	GRGCYC	2 cut(s)	734, 815
EgeI	GGCGCC	2 cut(s)	663, 1042
EheI	GGCGCC	2 cut(s)	663, 1042
ErhI	CCWWGG	1 cut(s)	126
FaeI	CATG	4 cut(s)	74, 130, 508, 976
FaiI	YATR	5 cut(s)	72, 128, 382, 506, 974
FatI	CATG	4 cut(s)	70, 126, 504, 972
FauI	CCCGC	9 cut(s)	37, 90, 120, 179, 191, 204, 288, 313, 1017
FbaI	TGATCA	1 cut(s)	357
Fnu4HI	GCNGC	7 cut(s)	241, 464, 512, 588, 899, 964, 1064
FokI	GGATG	5 cut(s)	64, 179, 244, 844, 937
FriOI	GRGCYC	2 cut(s)	734, 815
Fsp4HI	GCNGC	7 cut(s)	241, 464, 512, 588, 899, 964, 1064
GlaI	GCGC	7 cut(s)	418, 636, 663, 706, 1027, 1042, 1158
GluI	GCNGC	7 cut(s)	241, 464, 512, 588, 899, 964, 1064
GsaI	CCCAGC	1 cut(s)	606
GsuI	CTGGAG	1 cut(s)	910
HaeII	RGCGCY	3 cut(s)	665, 1044, 1160
HaeIII	GGCC	5 cut(s)	342, 545, 587, 601, 901
HgaI	GACGC	3 cut(s)	228, 807, 955
HhaI	GCGC	7 cut(s)	419, 637, 664, 707, 1028, 1043, 1159
Hin1I	GRCGYC	4 cut(s)	662, 818, 966, 1041
Hin1II	CATG	4 cut(s)	74, 130, 508, 976
Hin6I	GCGC	7 cut(s)	417, 635, 662, 705, 1026, 1041, 1157
HinP1I	GCGC	7 cut(s)	417, 635, 662, 705, 1026, 1041, 1157
HincII	GTYRAC	2 cut(s)	493, 921
HindII	GTYRAC	2 cut(s)	493, 921
HindIII	AAGCTT	1 cut(s)	883
HinfI	GANTC	9 cut(s)	275, 290, 350, 563, 671, 801, 933, 1019, 1032
HphI	GGTGA	6 cut(s)	49, 278, 367, 679, 850, 852
Hpy166II	GTNNAC	2 cut(s)	493, 921
Hpy188I	TCNGA	6 cut(s)	676, 800, 947, 1018, 1089, 1097
Hpy188III	TCNNGA	5 cut(s)	560, 625, 697, 889, 1151
Hpy8I	GTNNAC	2 cut(s)	493, 921
HpyAV	CCTTC	2 cut(s)	160, 952
HpyCH4III	ACNGT	3 cut(s)	530, 619, 864
HpyCH4IV	ACGT	5 cut(s)	189, 324, 453, 480, 489
HpyCH4V	TGCA	4 cut(s)	11, 26, 74, 498
HpyF10VI	GCNNNNNNNGC	8 cut(s)	240, 414, 423, 891, 904, 969, 999, 1165
HpyF3I	CTNAG	2 cut(s)	404, 1136
HpySE526I	ACGT	5 cut(s)	189, 324, 453, 480, 489
Hsp92I	GRCGYC	4 cut(s)	662, 818, 966, 1041
Hsp92II	CATG	4 cut(s)	74, 130, 508, 976
HspAI	GCGC	7 cut(s)	417, 635, 662, 705, 1026, 1041, 1157
KasI	GGCGCC	2 cut(s)	661, 1040
Kpn2I	TCCGGA	3 cut(s)	559, 624, 1150
KpnI	GGTACC	1 cut(s)	874
KroI	GCCGGC	1 cut(s)	1131
KroNI	GCCGGC	1 cut(s)	1133
Ksp22I	TGATCA	1 cut(s)	357
LmnI	GCTCC	8 cut(s)	248, 627, 712, 729, 737, 810, 844, 891
Lsp1109I	GCAGC	2 cut(s)	450, 498
LweI	GCATC	5 cut(s)	20, 83, 507, 639, 866
MaeII	ACGT	5 cut(s)	189, 324, 453, 480, 489
MaeIII	GTNAC	5 cut(s)	160, 266, 410, 685, 838
MbiI	CCGCTC	2 cut(s)	243, 808
MboII	GAAGA	5 cut(s)	43, 928, 979, 997, 1110
MflI	RGATCY	1 cut(s)	1147
MhlI	GDGCHC	2 cut(s)	734, 815
Mly113I	GGCGCC	2 cut(s)	662, 1041
MlyI	GAGTC	5 cut(s)	269, 284, 359, 942, 1028
MmeI	TCCRAC	2 cut(s)	238, 759
Mph1103I	ATGCAT	1 cut(s)	76
MroI	TCCGGA	3 cut(s)	559, 624, 1150
MroNI	GCCGGC	1 cut(s)	1131
MseI	TTAA	3 cut(s)	119, 779, 1179
MslI	CAYNNNNRTG	3 cut(s)	131, 503, 862
MspA1I	CMGCKG	1 cut(s)	1012
MspR9I	CCNGG	1 cut(s)	542
MvnI	CGCG	4 cut(s)	635, 707, 1028, 1063
MwoI	GCNNNNNNNGC	8 cut(s)	240, 414, 423, 891, 904, 969, 999, 1165
NaeI	GCCGGC	1 cut(s)	1133
NarI	GGCGCC	2 cut(s)	662, 1041
NciI	CCSGG	1 cut(s)	542
NcoI	CCATGG	1 cut(s)	126
NgoMIV	GCCGGC	1 cut(s)	1131
NlaIII	CATG	4 cut(s)	74, 130, 508, 976
NlaIV	GGNNCC	6 cut(s)	227, 663, 846, 872, 1042, 1048
NmeAIII	GCCGAG	2 cut(s)	747, 794
NmuCI	GTSAC	5 cut(s)	160, 266, 410, 685, 838
NsiI	ATGCAT	1 cut(s)	76
NspI	RCATGY	1 cut(s)	508
NspV	TTCGAA	1 cut(s)	457
PaeR7I	CTCGAG	2 cut(s)	638, 930
PciI	ACATGT	1 cut(s)	504
PcsI	WCGNNNNNNNCGW	9 cut(s)	129, 270, 306, 321, 345, 450, 486, 848, 860
PdiI	GCCGGC	1 cut(s)	1133
PfeI	GAWTC	4 cut(s)	563, 671, 801, 1032
PflMI	CCANNNNNTGG	1 cut(s)	741
PinAI	ACCGGT	1 cut(s)	1049
PkrI	GCNGC	7 cut(s)	242, 465, 513, 589, 900, 965, 1065
Ple19I	CGATCG	1 cut(s)	719
PleI	GAGTC	5 cut(s)	269, 284, 358, 941, 1027
PluTI	GGCGCC	2 cut(s)	665, 1044
PmaCI	CACGTG	2 cut(s)	325, 481
PmlI	CACGTG	2 cut(s)	325, 481
PpsI	GAGTC	5 cut(s)	269, 284, 358, 941, 1027
Ppu21I	YACGTR	2 cut(s)	325, 481
PscI	ACATGT	1 cut(s)	504
Psp124BI	GAGCTC	2 cut(s)	734, 815
PspCI	CACGTG	2 cut(s)	325, 481
PspFI	CCCAGC	1 cut(s)	602
PspN4I	GGNNCC	6 cut(s)	227, 663, 846, 872, 1042, 1048
PspPI	GGNCC	2 cut(s)	543, 600
PspXI	VCTCGAGB	2 cut(s)	638, 930
PsuI	RGATCY	1 cut(s)	1147
PvuI	CGATCG	1 cut(s)	719
RgaI	GCGATCGC	1 cut(s)	719
RsaI	GTAC	2 cut(s)	95, 872
RsaNI	GTAC	2 cut(s)	94, 871
RseI	CAYNNNNRTG	3 cut(s)	131, 503, 862
SacI	GAGCTC	2 cut(s)	734, 815
SaqAI	TTAA	3 cut(s)	119, 779, 1179
SatI	GCNGC	7 cut(s)	241, 464, 512, 588, 899, 964, 1064
Sau96I	GGNCC	2 cut(s)	543, 600
SchI	GAGTC	5 cut(s)	269, 284, 359, 942, 1028
ScrFI	CCNGG	1 cut(s)	542
SduI	GDGCHC	2 cut(s)	734, 815
SfaAI	GCGATCGC	1 cut(s)	719
SfaNI	GCATC	5 cut(s)	20, 83, 507, 639, 866
SfcI	CTRYAG	1 cut(s)	955
SfoI	GGCGCC	2 cut(s)	663, 1042
Sfr274I	CTCGAG	2 cut(s)	638, 930
SfuI	TTCGAA	1 cut(s)	457
SgfI	GCGATCGC	1 cut(s)	719
SlaI	CTCGAG	2 cut(s)	638, 930
SmiMI	CAYNNNNRTG	3 cut(s)	131, 503, 862
SmlI	CTYRAG	2 cut(s)	638, 930
SmoI	CTYRAG	2 cut(s)	638, 930
SspDI	GGCGCC	2 cut(s)	661, 1040
SspI	AATATT	1 cut(s)	552
SstI	GAGCTC	2 cut(s)	734, 815
StyD4I	CCNGG	1 cut(s)	540
StyI	CCWWGG	1 cut(s)	126
TaaI	ACNGT	3 cut(s)	530, 619, 864
TaiI	ACGT	5 cut(s)	192, 327, 456, 483, 492
TauI	GCSGC	5 cut(s)	243, 590, 901, 966, 1066
TfiI	GAWTC	4 cut(s)	563, 671, 801, 1032
Tru1I	TTAA	3 cut(s)	119, 779, 1179
Tru9I	TTAA	3 cut(s)	119, 779, 1179
TscAI	CASTG	1 cut(s)	622
TseFI	GTSAC	5 cut(s)	160, 266, 410, 685, 838
TseI	GCWGC	2 cut(s)	463, 511
Tsp45I	GTSAC	5 cut(s)	160, 266, 410, 685, 838
TspDTI	ATGAA	2 cut(s)	43, 989
TspGWI	ACGGA	3 cut(s)	545, 567, 857
TspRI	CASTG	1 cut(s)	622
Van91I	CCANNNNNTGG	1 cut(s)	741
XceI	RCATGY	1 cut(s)	508
XhoI	CTCGAG	2 cut(s)	638, 930
Zsp2I	ATGCAT	1 cut(s)	76

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.