Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007392

Physical Location & Seq

Genomic Coordinates Forward (+)

18560071 .. 18565038

Gene Structure 4968 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1112200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2004 bp

ATGTCCCTCCTCCGCAGAACCTTCCCTCTCCTCCCCCTCCGCCCCAAACACCTCCCTCTCCTATCCCACTCCTTCCTTTTCCTCAAACCATCGTTCCCTAAACCCCTTCCCAAACACACCTCCATCTTCTCTTCCTCCGCTTCCGCTTCCACTTCCACTTCCACTTCCACACCCATTCCCAATCCCACCTACAATCCCCCAATTTCCCCAACCCAACTCCAATGGGTCACCAGAACCGCCTCCTGCGGCGACCTATCCACCGCCGACGTCGGCAAAAGGGTCACTCTCTGCGGGTGGGTCGCCCTCCACCGTGTCCACGGCGGCCTCACCTTTCTCAACCTCCGTGACCACACCGGTTCCGTCCAGGTCACCACTCTCCCCGACACTTTCCCCGACGCTCATTCCGCCGTTAACGACCTGAGGCTCGAGTACGTCGTAGCCATTGAAGGACTCGTCCGGTCTAGACCGGCCGACTCCATCAACAAGAAGATGAAAACCGGGTTAATTGAGGTTGCGGCGGAGAGCGTTCAAGTGTTGAATGCCGTTAGAACAAAGCTGCCATTTTTGGTCACTACTGCTGATGATGCTAAGGATTCTGCAAAGGAGGAGATACGGTTAAGGTACCGGTGCCTTGATTTACGCAGGCAGCAAATGCATTCGAACATAATGTTGCGGCATAAAGTTGTGAAATTGATTCGAAGACATCTAGAGGATGCACATGGTTTTGTGGAGATTGAGACTCCAATACTCTCTAGGTCTACACCCGAAGGTGCTCGAGATTATTTAGTGCCCTCGAGAGTTCAGCCAGGAACATTTTATGCTTTGCCTCAAAGTCCGCAACTGTTCAAGCAAATGTTGATGGTTTCTGGTTTTGATAAGTATTATCAAATAGCAAGGTGCTTTCGAGATGAAGATCTAAGAGCTGATAGACAACCTGAGTTCACACAGCTTGATATGGAGCTTGCTTTCACTCCTTTGGAGGAGATGCTGAAGCTTAATGAAGATTTGATTAGAAAGGTTTTTCTAGAGATCAAAGGCGTAAATCTCCCAAACCCTTTTCCTAGGCTTACGTATGCTGAAGCAATGAGTCGATATGGTTCGGACAGGCCAGATATTAGATTTGAACTAGAGTTAAAAGATGTATCTGATATTTTCTCAGATTCCCCGTTCCGGGTTTTTGCTGATACTTTGAAAAGTGGGGGAGTTATCAAAGTTTTATGCATACCTTCAGGTGGTAAAAGATATTCAAACACGGCGCTAAAGAAGGGCGATGTTTACAATGAAGCAATCAAATCTGGAGCAAAGGGTTTGCCTTTCCTGAAGGTCTTGAATGATGGCGGAATGGAGGGAATTCCTGCATTGGTATCTAGTTTGGACCCAACAAAGACACAGCAGTTTCTGAGTCGTTGCTCTGCCAGACCAGGTGATCTTATCTTGTTTGCAGTGGGTCATCATACATCGGTTAATAAAACTCTAGATCGACTACGGCTCTTTATGGCACATGAACTTGGTTTGGTTGATGATTCGAGGCATTCAATTCTATGGGTGACTGATTTCCCAATGTTTGAATGGAATGATTCTGAGCAAAGGCTTGAGTCCTTGCACCACCCTTTCACTGCCCCGAATCCTGAAGACATACAAGATCTGTCTTCTGCTCGTGCTTTAGCTTATGACATGGTTTACAATGGAGTTGAGATTGGTGGGGGAAGTTTGAGAATTTATAAACGTGATGTACAGCAAAAGGTTTTGGAGATTGTTGGCATCTCCCCTGAAGAGGCTGAAACGAAGTTTGGCTATCTTCTTGAGGCATTAGACATGGGTGCCCCTCCACATGGGGGAATTGCATATGGTTTGGACAGATTGGTTATGTTGTTAGCAGGTGCTAATTCCATTAGGGACGTCATAGCTTTCCCAAAGACAACCACCGCACAGTGTGCTCTCACTCGAACACCATCTCCAGTGGATCCCCAGCAGCTAAAGGACCTATCATACCGGACTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

GAD tRNA-synt_2 tRNA_anti-codon

Protein Analysis

668

Amino Acids

74.52

Weight (kDa)

8.47

Isoelectric Point (pI)

47.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_anti-codon	PF01336	94 - 179	3.2e-12	OB-fold nucleic acid binding domain
tRNA-synt_2	PF00152	201 - 640	1.8e-122	tRNA synthetases class II (D, K and N)
GAD	PF02938	393 - 492	3.8e-17	GAD domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007392)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33760 AT4G33760
fragaria_vesca	FvH4_2g03040 FvH4_2g03040 FvH4_2g03040 FvH4_2g03040 FvH4_2g03040 FvH4_2g03040 FvH4_2g03040 FvH4_2g03040
malus_domestica	MD10G1112200.v1.1
prunus_persica	Prupe.8G151800_v2.0.a1
pyrus_communis	pycom10g09720
rosa_chinensis	RchiOBHm_Chr6g0247831
rosa_laevigata	RLG00000015175
rosa_multiflora	Rmu_sc0001048.1_g000005 Rmu_sc0035971.1_g000001
rosa_roxburghii	Rroxscaffold_180G00433200 Rroxscaffold_7G00213610
rosa_rugosa	Rorug05G0529400
rosa_samantha	Rh6AG044100 Rh6BG038800 Rh6CG037600 Rh6DG034600
rosa_wichuraiana	Rw6G003820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1722
AarI	CACCTGC	1 cut(s)	1868
AatII	GACGTC	2 cut(s)	270, 1902
Acc36I	ACCTGC	1 cut(s)	1868
Acc65I	GGTACC	1 cut(s)	621
AccB1I	GGYRCC	3 cut(s)	621, 627, 1820
AccI	GTMKAC	1 cut(s)	758
AclWI	GGATC	2 cut(s)	1958, 1971
AcoI	YGGCCR	1 cut(s)	468
AcsI	RAATTY	2 cut(s)	1350, 1716
AcuI	CTGAAG	6 cut(s)	1010, 1098, 1212, 1340, 1650, 1791
AcyI	GRCGYC	2 cut(s)	267, 1899
AdeI	CACNNNGTG	1 cut(s)	1934
AfaI	GTAC	3 cut(s)	431, 623, 1734
AfiI	CCNNNNNNNGG	6 cut(s)	1170, 1171, 1232, 1774, 1832, 1835
AgeI	ACCGGT	2 cut(s)	353, 624
AjnI	CCWGG	3 cut(s)	363, 805, 1420
AjuI	GAANNNNNNNTTGG	2 cut(s)	1773, 1805
AloI	GAACNNNNNNTCC	2 cut(s)	1939, 1971
AluBI	AGCT	8 cut(s)	556, 923, 949, 961, 994, 1667, 1907, 1975
AluI	AGCT	8 cut(s)	556, 923, 949, 961, 994, 1667, 1907, 1975
Alw21I	GWGCWC	2 cut(s)	775, 1939
Alw26I	GTCTC	1 cut(s)	731
AlwI	GGATC	2 cut(s)	1958, 1971
AlwNI	CAGNNNCTG	1 cut(s)	1399
Ama87I	CYCGRG	3 cut(s)	425, 774, 793
AoxI	GGCC	3 cut(s)	322, 468, 1106
ApeKI	GCWGC	3 cut(s)	556, 646, 1972
ApoI	RAATTY	2 cut(s)	1350, 1716
AsiGI	ACCGGT	2 cut(s)	353, 624
Asp718I	GGTACC	1 cut(s)	621
AspA2I	CCTAGG	1 cut(s)	1061
AspLEI	GCGC	1 cut(s)	1258
AspS9I	GGNCC	2 cut(s)	1375, 1981
AsuC2I	CCSGG	2 cut(s)	499, 1172
AsuHPI	GGTGA	5 cut(s)	220, 319, 361, 1436, 1558
AsuII	TTCGAA	2 cut(s)	659, 697
AvaI	CYCGRG	3 cut(s)	425, 774, 793
AvaII	GGWCC	2 cut(s)	1375, 1981
AvrII	CCTAGG	1 cut(s)	1061
AxyI	CCTNAGG	1 cut(s)	419
BaeGI	GKGCMC	2 cut(s)	792, 1825
BamHI	GGATCC	1 cut(s)	1963
BanI	GGYRCC	3 cut(s)	621, 627, 1820
BauI	CACGAG	1 cut(s)	1656
BbsI	GAAGAC	3 cut(s)	706, 1638, 1641
Bbv12I	GWGCWC	2 cut(s)	775, 1939
BbvI	GCAGC	3 cut(s)	543, 658, 1984
BccI	CCATC	6 cut(s)	97, 131, 485, 853, 1328, 1960
BceAI	ACGGC	5 cut(s)	334, 392, 527, 1269, 1502
BciT130I	CCWGG	3 cut(s)	365, 807, 1422
BcnI	CCSGG	2 cut(s)	499, 1172
BcoDI	GTCTC	1 cut(s)	731
BfaI	CTAG	8 cut(s)	462, 707, 753, 1025, 1062, 1127, 1368, 1475
BfoI	RGCGCY	1 cut(s)	1259
BfuAI	ACCTGC	1 cut(s)	1868
BglII	AGATCT	2 cut(s)	913, 1642
BisI	GCNGC	7 cut(s)	247, 322, 516, 557, 647, 674, 1973
BlnI	CCTAGG	1 cut(s)	1061
BlsI	GCNGC	7 cut(s)	248, 323, 517, 558, 648, 675, 1974
Bme1390I	CCNGG	5 cut(s)	365, 499, 807, 1172, 1422
Bme18I	GGWCC	2 cut(s)	1375, 1981
BmeT110I	CYCGRG	3 cut(s)	425, 774, 793
BmgT120I	GGNCC	2 cut(s)	1375, 1981
BmiI	GGNNCC	6 cut(s)	358, 623, 629, 1377, 1822, 1965
BmrFI	CCNGG	5 cut(s)	365, 499, 807, 1172, 1422
BmsI	GCATC	4 cut(s)	574, 703, 975, 1770
BpiI	GAAGAC	3 cut(s)	706, 1638, 1641
BpmI	CTGGAG	2 cut(s)	1317, 1941
Bpu10I	CCTNAGC	1 cut(s)	588
Bpu14I	TTCGAA	2 cut(s)	659, 697
BpuEI	CTTGAG	2 cut(s)	1613, 1823
BpuMI	CCSGG	2 cut(s)	499, 1172
BsaAI	YACGTR	1 cut(s)	1071
BsaBI	GATNNNNATC	1 cut(s)	912
BsaHI	GRCGYC	2 cut(s)	267, 1899
BsaJI	CCNNGG	2 cut(s)	316, 1061
BsaWI	WCCGGW	4 cut(s)	353, 456, 624, 1992
BsaXI	ACNNNNNCTCC	4 cut(s)	599, 629, 1939, 1969
Bsc4I	CCNNNNNNNGG	6 cut(s)	1170, 1171, 1232, 1774, 1832, 1835
Bse118I	RCCGGY	3 cut(s)	353, 466, 624
Bse1I	ACTGG	1 cut(s)	1958
Bse21I	CCTNAGG	1 cut(s)	419
Bse3DI	GCAATG	1 cut(s)	1089
Bse8I	GATNNNNATC	1 cut(s)	912
BseBI	CCWGG	3 cut(s)	365, 807, 1422
BseDI	CCNNGG	2 cut(s)	316, 1061
BseGI	GGATG	1 cut(s)	718
BseJI	GATNNNNATC	1 cut(s)	912
BseLI	CCNNNNNNNGG	6 cut(s)	1170, 1171, 1232, 1774, 1832, 1835
BseMI	GCAATG	1 cut(s)	1089
BseMII	CTCAG	5 cut(s)	410, 927, 1170, 1391, 1572
BseNI	ACTGG	1 cut(s)	1958
BseRI	GAGGAG	3 cut(s)	20, 620, 995
BseSI	GKGCMC	2 cut(s)	792, 1825
BseX3I	CGGCCG	1 cut(s)	468
BseXI	GCAGC	3 cut(s)	543, 658, 1984
BseYI	CCCAGC	1 cut(s)	1968
Bsh1285I	CGRYCG	1 cut(s)	471
BshFI	GGCC	3 cut(s)	324, 470, 1108
BshNI	GGYRCC	3 cut(s)	621, 627, 1820
BshTI	ACCGGT	2 cut(s)	353, 624
BsiEI	CGRYCG	1 cut(s)	471
BsiHKAI	GWGCWC	2 cut(s)	775, 1939
BsiHKCI	CYCGRG	3 cut(s)	425, 774, 793
BsiSI	CCGG	7 cut(s)	354, 457, 467, 498, 625, 1171, 1993
BslFI	GGGAC	1 cut(s)	1910
BslI	CCNNNNNNNGG	6 cut(s)	1170, 1171, 1232, 1774, 1832, 1835
BsmAI	GTCTC	1 cut(s)	731
BsmFI	GGGAC	1 cut(s)	1910
BsmI	GAATGC	3 cut(s)	544, 655, 1531
BsnI	GGCC	3 cut(s)	324, 470, 1108
BsoBI	CYCGRG	3 cut(s)	425, 774, 793
Bsp119I	TTCGAA	2 cut(s)	659, 697
Bsp1286I	GDGCHC	4 cut(s)	775, 792, 1825, 1939
Bsp1407I	TGTACA	1 cut(s)	1732
Bsp143I	GATC	6 cut(s)	913, 1029, 1426, 1477, 1642, 1963
BspANI	GGCC	3 cut(s)	324, 470, 1108
BspCNI	CTCAG	5 cut(s)	411, 928, 1169, 1392, 1573
BspLI	GGNNCC	6 cut(s)	358, 623, 629, 1377, 1822, 1965
BspMI	ACCTGC	1 cut(s)	1868
BspPI	GGATC	2 cut(s)	1958, 1971
BspT104I	TTCGAA	2 cut(s)	659, 697
BspT107I	GGYRCC	3 cut(s)	621, 627, 1820
BsrDI	GCAATG	1 cut(s)	1089
BsrFI	RCCGGY	3 cut(s)	353, 466, 624
BsrGI	TGTACA	1 cut(s)	1732
BsrI	ACTGG	1 cut(s)	1958
BssAI	RCCGGY	3 cut(s)	353, 466, 624
BssECI	CCNNGG	2 cut(s)	316, 1061
BssMI	GATC	6 cut(s)	913, 1029, 1426, 1477, 1642, 1963
BssNI	GRCGYC	2 cut(s)	267, 1899
BssSI	CACGAG	1 cut(s)	1656
BssT1I	CCWWGG	1 cut(s)	1061
Bst2BI	CACGAG	1 cut(s)	1656
Bst2UI	CCWGG	3 cut(s)	365, 807, 1422
Bst4CI	ACNGT	4 cut(s)	311, 615, 843, 1932
Bst6I	CTCTTC	2 cut(s)	136, 1767
BstACI	GRCGYC	2 cut(s)	267, 1899
BstAPI	GCANNNNNTGC	2 cut(s)	652, 1934
BstAUI	TGTACA	1 cut(s)	1732
BstBAI	YACGTR	1 cut(s)	1071
BstBI	TTCGAA	2 cut(s)	659, 697
BstC8I	GCNNGC	2 cut(s)	644, 963
BstDEI	CTNAG	8 cut(s)	419, 588, 917, 936, 1156, 1400, 1581, 1998
BstDSI	CCRYGG	1 cut(s)	316
BstEII	GGTNACC	2 cut(s)	226, 367
BstF5I	GGATG	1 cut(s)	718
BstH2I	RGCGCY	1 cut(s)	1259
BstHHI	GCGC	1 cut(s)	1258
BstKTI	GATC	6 cut(s)	916, 1032, 1429, 1480, 1645, 1966
BstMAI	GTCTC	1 cut(s)	731
BstMBI	GATC	6 cut(s)	913, 1029, 1426, 1477, 1642, 1963
BstMCI	CGRYCG	1 cut(s)	471
BstMWI	GCNNNNNNNGC	4 cut(s)	404, 584, 652, 1934
BstNI	CCWGG	3 cut(s)	365, 807, 1422
BstPI	GGTNACC	2 cut(s)	226, 367
BstSCI	CCNGG	5 cut(s)	363, 497, 805, 1170, 1420
BstSLI	GKGCMC	2 cut(s)	792, 1825
BstSNI	TACGTA	1 cut(s)	1071
BstV1I	GCAGC	3 cut(s)	543, 658, 1984
BstV2I	GAAGAC	3 cut(s)	706, 1638, 1641
BstX2I	RGATCY	3 cut(s)	913, 1642, 1963
BstYI	RGATCY	3 cut(s)	913, 1642, 1963
BstZI	CGGCCG	1 cut(s)	468
Bsu36I	CCTNAGG	1 cut(s)	419
BsuRI	GGCC	3 cut(s)	324, 470, 1108
BtgI	CCRYGG	1 cut(s)	316
BtgZI	GCGATG	1 cut(s)	1284
BtsCI	GGATG	1 cut(s)	718
BtsI	GCAGTG	2 cut(s)	1449, 1614
BtsIMutI	CAGTG	4 cut(s)	1449, 1614, 1937, 1965
BveI	ACCTGC	1 cut(s)	1868
Cac8I	GCNNGC	2 cut(s)	644, 963
CaiI	CAGNNNCTG	1 cut(s)	1399
CfoI	GCGC	1 cut(s)	1258
Cfr10I	RCCGGY	3 cut(s)	353, 466, 624
Cfr13I	GGNCC	2 cut(s)	1375, 1981
CseI	GACGC	1 cut(s)	404
CsiI	ACCWGGT	1 cut(s)	1420
Csp6I	GTAC	3 cut(s)	430, 622, 1733
CspAI	ACCGGT	2 cut(s)	353, 624
CviAII	CATG	5 cut(s)	719, 1502, 1675, 1816, 1832
CviQI	GTAC	3 cut(s)	430, 622, 1733
DdeI	CTNAG	8 cut(s)	419, 588, 917, 936, 1156, 1400, 1581, 1998
DpnI	GATC	6 cut(s)	915, 1031, 1428, 1479, 1644, 1965
DpnII	GATC	6 cut(s)	913, 1029, 1426, 1477, 1642, 1963
DraIII	CACNNNGTG	1 cut(s)	1934
EaeI	YGGCCR	1 cut(s)	468
EagI	CGGCCG	1 cut(s)	468
Eam1104I	CTCTTC	2 cut(s)	136, 1767
EarI	CTCTTC	2 cut(s)	136, 1767
EciI	GGCGGA	4 cut(s)	29, 394, 533, 1353
EclXI	CGGCCG	1 cut(s)	468
Eco105I	TACGTA	1 cut(s)	1071
Eco130I	CCWWGG	1 cut(s)	1061
Eco47I	GGWCC	2 cut(s)	1375, 1981
Eco52I	CGGCCG	1 cut(s)	468
Eco57I	CTGAAG	6 cut(s)	1010, 1098, 1212, 1340, 1650, 1791
Eco81I	CCTNAGG	1 cut(s)	419
Eco88I	CYCGRG	3 cut(s)	425, 774, 793
Eco91I	GGTNACC	2 cut(s)	226, 367
EcoO109I	RGGNCCY	1 cut(s)	1981
EcoO65I	GGTNACC	2 cut(s)	226, 367
EcoRI	GAATTC	1 cut(s)	1350
EcoRII	CCWGG	3 cut(s)	363, 805, 1420
EcoT14I	CCWWGG	1 cut(s)	1061
EcoT22I	ATGCAT	2 cut(s)	657, 1223
ErhI	CCWWGG	1 cut(s)	1061
FaeI	CATG	5 cut(s)	722, 1505, 1678, 1819, 1835
FaqI	GGGAC	1 cut(s)	1910
FatI	CATG	5 cut(s)	718, 1501, 1674, 1815, 1831
FauI	CCCGC	1 cut(s)	284
FauNDI	CATATG	1 cut(s)	1846
FblI	GTMKAC	1 cut(s)	758
Fnu4HI	GCNGC	7 cut(s)	247, 322, 516, 557, 647, 674, 1973
FokI	GGATG	1 cut(s)	725
Fsp4HI	GCNGC	7 cut(s)	247, 322, 516, 557, 647, 674, 1973
FspBI	CTAG	8 cut(s)	462, 707, 753, 1025, 1062, 1127, 1368, 1475
GlaI	GCGC	1 cut(s)	1257
GluI	GCNGC	7 cut(s)	247, 322, 516, 557, 647, 674, 1973
GsaI	CCCAGC	1 cut(s)	1972
GsuI	CTGGAG	2 cut(s)	1317, 1941
HaeII	RGCGCY	1 cut(s)	1259
HaeIII	GGCC	3 cut(s)	324, 470, 1108
HapII	CCGG	7 cut(s)	354, 457, 467, 498, 625, 1171, 1993
HgaI	GACGC	1 cut(s)	404
HhaI	GCGC	1 cut(s)	1258
Hin1I	GRCGYC	2 cut(s)	267, 1899
Hin1II	CATG	5 cut(s)	722, 1505, 1678, 1819, 1835
Hin6I	GCGC	1 cut(s)	1256
HinP1I	GCGC	1 cut(s)	1256
HincII	GTYRAC	1 cut(s)	412
HindII	GTYRAC	1 cut(s)	412
HindIII	AAGCTT	1 cut(s)	992
HpaI	GTTAAC	1 cut(s)	412
HpaII	CCGG	7 cut(s)	354, 457, 467, 498, 625, 1171, 1993
HphI	GGTGA	5 cut(s)	220, 319, 361, 1436, 1558
Hpy166II	GTNNAC	6 cut(s)	316, 412, 759, 942, 1276, 1681
Hpy188I	TCNGA	5 cut(s)	1102, 1147, 1159, 1401, 1582
Hpy8I	GTNNAC	6 cut(s)	316, 412, 759, 942, 1276, 1681
Hpy99I	CGWCG	4 cut(s)	269, 272, 398, 437
HpyAV	CCTTC	8 cut(s)	31, 82, 116, 440, 761, 1236, 1258, 1315
HpyCH4III	ACNGT	4 cut(s)	311, 615, 843, 1932
HpyCH4IV	ACGT	5 cut(s)	267, 432, 1070, 1726, 1899
HpyCH4V	TGCA	8 cut(s)	599, 655, 716, 1221, 1358, 1442, 1603, 1844
HpyF10VI	GCNNNNNNNGC	4 cut(s)	404, 584, 652, 1934
HpyF3I	CTNAG	8 cut(s)	419, 588, 917, 936, 1156, 1400, 1581, 1998
HpySE526I	ACGT	5 cut(s)	267, 432, 1070, 1726, 1899
Hsp92I	GRCGYC	2 cut(s)	267, 1899
Hsp92II	CATG	5 cut(s)	722, 1505, 1678, 1819, 1835
HspAI	GCGC	1 cut(s)	1256
KpnI	GGTACC	1 cut(s)	625
KspAI	GTTAAC	1 cut(s)	412
Kzo9I	GATC	6 cut(s)	913, 1029, 1426, 1477, 1642, 1963
LmnI	GCTCC	2 cut(s)	958, 1298
Lsp1109I	GCAGC	3 cut(s)	543, 658, 1984
LweI	GCATC	4 cut(s)	574, 703, 975, 1770
MabI	ACCWGGT	1 cut(s)	1420
MaeI	CTAG	8 cut(s)	462, 707, 753, 1025, 1062, 1127, 1368, 1475
MaeII	ACGT	5 cut(s)	267, 432, 1070, 1726, 1899
MaeIII	GTNAC	6 cut(s)	226, 280, 344, 367, 568, 1546
MalI	GATC	6 cut(s)	915, 1031, 1428, 1479, 1644, 1965
MboI	GATC	6 cut(s)	913, 1029, 1426, 1477, 1642, 1963
MflI	RGATCY	3 cut(s)	913, 1642, 1963
MhlI	GDGCHC	4 cut(s)	775, 792, 1825, 1939
MluCI	AATT	8 cut(s)	201, 504, 689, 1350, 1536, 1716, 1839, 1885
MlyI	GAGTC	7 cut(s)	444, 467, 733, 1096, 1411, 1604, 1990
Mph1103I	ATGCAT	2 cut(s)	657, 1223
MseI	TTAA	6 cut(s)	411, 503, 617, 996, 1133, 1464
MspI	CCGG	7 cut(s)	354, 457, 467, 498, 625, 1171, 1993
MspR9I	CCNGG	5 cut(s)	365, 499, 807, 1172, 1422
Mva1269I	GAATGC	3 cut(s)	544, 655, 1531
MvaI	CCWGG	3 cut(s)	365, 807, 1422
MwoI	GCNNNNNNNGC	4 cut(s)	404, 584, 652, 1934
NciI	CCSGG	2 cut(s)	499, 1172
NdeI	CATATG	1 cut(s)	1846
NdeII	GATC	6 cut(s)	913, 1029, 1426, 1477, 1642, 1963
NlaIII	CATG	5 cut(s)	722, 1505, 1678, 1819, 1835
NlaIV	GGNNCC	6 cut(s)	358, 623, 629, 1377, 1822, 1965
NmuCI	GTSAC	6 cut(s)	226, 280, 344, 367, 568, 1546
NsiI	ATGCAT	2 cut(s)	657, 1223
NspV	TTCGAA	2 cut(s)	659, 697
PaeR7I	CTCGAG	3 cut(s)	425, 774, 793
PaqCI	CACCTGC	1 cut(s)	1868
PcsI	WCGNNNNNNNCGW	1 cut(s)	432
PctI	GAATGC	3 cut(s)	544, 655, 1531
PfeI	GAWTC	6 cut(s)	593, 694, 1160, 1523, 1577, 1624
PinAI	ACCGGT	2 cut(s)	353, 624
PkrI	GCNGC	7 cut(s)	248, 323, 517, 558, 648, 675, 1974
PleI	GAGTC	7 cut(s)	444, 467, 733, 1095, 1410, 1603, 1990
PpsI	GAGTC	7 cut(s)	444, 467, 733, 1095, 1410, 1603, 1990
Ppu21I	YACGTR	1 cut(s)	1071
PpuMI	RGGWCCY	1 cut(s)	1981
PsiI	TTATAA	1 cut(s)	1722
Psp5II	RGGWCCY	1 cut(s)	1981
Psp6I	CCWGG	3 cut(s)	363, 805, 1420
PspEI	GGTNACC	2 cut(s)	226, 367
PspFI	CCCAGC	1 cut(s)	1968
PspGI	CCWGG	3 cut(s)	363, 805, 1420
PspN4I	GGNNCC	6 cut(s)	358, 623, 629, 1377, 1822, 1965
PspPI	GGNCC	2 cut(s)	1375, 1981
PspPPI	RGGWCCY	1 cut(s)	1981
PspXI	VCTCGAGB	1 cut(s)	425
PstNI	CAGNNNCTG	1 cut(s)	1399
PsuI	RGATCY	3 cut(s)	913, 1642, 1963
RsaI	GTAC	3 cut(s)	431, 623, 1734
RsaNI	GTAC	3 cut(s)	430, 622, 1733
SaqAI	TTAA	6 cut(s)	411, 503, 617, 996, 1133, 1464
SatI	GCNGC	7 cut(s)	247, 322, 516, 557, 647, 674, 1973
Sau3AI	GATC	6 cut(s)	913, 1029, 1426, 1477, 1642, 1963
Sau96I	GGNCC	2 cut(s)	1375, 1981
SchI	GAGTC	7 cut(s)	444, 467, 733, 1096, 1411, 1604, 1990
ScrFI	CCNGG	5 cut(s)	365, 499, 807, 1172, 1422
SduI	GDGCHC	4 cut(s)	775, 792, 1825, 1939
SexAI	ACCWGGT	1 cut(s)	1420
SfaNI	GCATC	4 cut(s)	574, 703, 975, 1770
Sfr274I	CTCGAG	3 cut(s)	425, 774, 793
SfuI	TTCGAA	2 cut(s)	659, 697
SinI	GGWCC	2 cut(s)	1375, 1981
SlaI	CTCGAG	3 cut(s)	425, 774, 793
SmlI	CTYRAG	5 cut(s)	425, 774, 793, 1592, 1802
SmoI	CTYRAG	5 cut(s)	425, 774, 793, 1592, 1802
SnaBI	TACGTA	1 cut(s)	1071
Sse9I	AATT	8 cut(s)	201, 504, 689, 1350, 1536, 1716, 1839, 1885
SspMI	CTAG	8 cut(s)	462, 707, 753, 1025, 1062, 1127, 1368, 1475
StyD4I	CCNGG	5 cut(s)	363, 497, 805, 1170, 1420
StyI	CCWWGG	1 cut(s)	1061
TaaI	ACNGT	4 cut(s)	311, 615, 843, 1932
TaiI	ACGT	5 cut(s)	270, 435, 1073, 1729, 1902
TasI	AATT	8 cut(s)	201, 504, 689, 1350, 1536, 1716, 1839, 1885
TatI	WGTACW	1 cut(s)	1732
TauI	GCSGC	4 cut(s)	249, 324, 518, 676
TfiI	GAWTC	6 cut(s)	593, 694, 1160, 1523, 1577, 1624
Tru1I	TTAA	6 cut(s)	411, 503, 617, 996, 1133, 1464
Tru9I	TTAA	6 cut(s)	411, 503, 617, 996, 1133, 1464
TscAI	CASTG	4 cut(s)	1449, 1621, 1937, 1965
TseFI	GTSAC	6 cut(s)	226, 280, 344, 367, 568, 1546
TseI	GCWGC	3 cut(s)	556, 646, 1972
Tsp45I	GTSAC	6 cut(s)	226, 280, 344, 367, 568, 1546
TspDTI	ATGAA	5 cut(s)	506, 924, 1014, 1296, 1518
TspGWI	ACGGA	2 cut(s)	332, 349
TspRI	CASTG	4 cut(s)	1449, 1621, 1937, 1965
VpaK11BI	GGWCC	2 cut(s)	1375, 1981
XapI	RAATTY	2 cut(s)	1350, 1716
XbaI	TCTAGA	4 cut(s)	461, 706, 1024, 1474
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1566
XhoI	CTCGAG	3 cut(s)	425, 774, 793
XmaJI	CCTAGG	1 cut(s)	1061
XmiI	GTMKAC	1 cut(s)	758
XspI	CTAG	8 cut(s)	462, 707, 753, 1025, 1062, 1127, 1368, 1475
ZraI	GACGTC	2 cut(s)	268, 1900
Zsp2I	ATGCAT	2 cut(s)	657, 1223

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.